CN115998781A - Application of birch juice separating liquid in treating gout - Google Patents
Application of birch juice separating liquid in treating gout Download PDFInfo
- Publication number
- CN115998781A CN115998781A CN202111230107.1A CN202111230107A CN115998781A CN 115998781 A CN115998781 A CN 115998781A CN 202111230107 A CN202111230107 A CN 202111230107A CN 115998781 A CN115998781 A CN 115998781A
- Authority
- CN
- China
- Prior art keywords
- birch juice
- birch
- nanofiltration
- separating liquid
- separation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention relates to a birch juice separating liquid and application thereof in treating gout. The birch juice separating liquid disclosed by the invention is prepared by an ultrafiltration-nanofiltration three-stage separation concentration process, the birch juice separating liquid consists of substances with relative molecular weight within the range of 500-700 dal in the birch juice, wherein the birch polysaccharide substance accounts for not less than 10%, and the prepared birch juice separating liquid has low reducing sugar, low protein and low fatty acid, is suitable for gout patients to take, can effectively reduce uric acid and creatinine levels in serum and gout joint swelling rate, lightens fibrous tissue hyperplasia, and can be used for treating gout diseases.
Description
Technical Field
The invention relates to a preparation method of a birch juice separating liquid and application thereof in treating gout, belonging to the technical field of medicines.
Background
Birch is a larch tree of the betulinaceae, and is mainly distributed in northeast, north-south and south-west areas of China. The natural birch juice is rich in monosaccharide, polysaccharide substances, amino acid, pentacyclic triterpene compounds, fatty acid saponins, proteins, etc., and has good biological effects. Birch juice is described in Jilin herbal medicine as useful: cough relieving. For cough and dyspnea due to phlegm. "in" Heilongjiang Manual of Chinese herbal medicine ", birch juice is described as having: "treat scurvy, kidney disease, gout". Has effects of clearing heat and detoxicating. Although birch juice is described in gout treatment, specific literature research and clinical use information of birch juice for gout treatment are not available at present, and pentacyclic triterpene compounds which are considered to be one of important bioactive components in the birch juice are not researched and reported to have the efficacy of treating gout, so that the research and development of effective substances and effective using means for treating gout in natural birch juice have urgent practical demands.
The natural birch juice contains a large amount of reducing saccharide substances such as monosaccharide, disaccharide and the like, the reducing saccharide enters the human body, the blood uric acid level is increased after the metabolism of glucokinase, the onset of gout diseases can be induced, and meanwhile, the components such as protein, free fatty acid and the like contained in the birch juice are not suitable for being ingested by gout patients, so that the components in the natural birch juice are removed through a separation and purification process, and the natural birch juice is more suitable for treating gout diseases and has important practical significance.
The ultrafiltration is a membrane separation technology for distinguishing substances with different molecular weights in a solution by utilizing the interception capability of a porous membrane and through a physical interception mode, and generally uses a pressure difference of 0.1-5 MPa as a driving force to promote the solution to pass through ultrafiltration membranes with different apertures, so that the purposes of purifying, concentrating and screening different components in the solution are achieved, and the method is suitable for macromolecular substances with molecular weights of 1500dal to 1000 kdal. The nanofiltration process is a pressure-driven membrane separation process between reverse osmosis and ultrafiltration, and is generally more suitable for separating substances with smaller relative molecular mass, such as small molecular inorganic substances, organic salts, monosaccharides, oligosaccharides and the like, compared with the ultrafiltration process, and can perform good separation on the small molecular substances with the relative molecular weight of 100-800 dal.
Disclosure of Invention
The invention relates to a birch juice separating liquid, which is prepared by carrying out ultrafiltration-nanofiltration three-stage separation and concentration on natural birch juice.
The birch juice separating liquid consists of substances with relative molecular weight of 500-700 dal in birch juice.
The total content of polysaccharide in the birch juice separating liquid is not less than 10%, preferably not less than 20%.
Through ultrafiltration-nanofiltration composite separation technology, components, such as macromolecular proteins, small molecular weight reducing sugar, free fatty acid and the like, which are not suitable for gout patients to ingest are separated from the birch juice, so that the birch juice separating liquid has the characteristics of high polysaccharide content, low reducing sugar, low protein and low fatty acid, and is suitable for gout patients to ingest.
The birch juice separating liquid can be used for treating gout diseases, and can be used in combination with other functional components to develop anti-gout products.
The birch juice separation concentrate referred to in the present invention is realized by the following technical means.
Under the conditions of pressure of 0.5-1.5 MPa, temperature of 25-45 ℃ and flow rate of 1-4 m/s, carrying out primary separation and purification on the birch juice stock solution by utilizing an ultrafiltration membrane component with molecular weight cut off of 1500dal, and collecting filtered liquid after the birch juice stock solution is circularly ultrafiltered and separated for 4h, thus obtaining the birch juice ultrafiltrate with content relative molecular weight lower than 1500 dal.
The birch juice ultrafiltrate is used as a raw material, the birch juice ultrafiltrate is subjected to secondary separation and purification by using a nanofiltration membrane component with 700dal interception molecular weight under the conditions of 0.5-1.5 MPa, 25-45 ℃ and flow rate of 1-4 m/s, and after 3 hours of cyclic nanofiltration separation, the filtered liquid is collected, so that the birch juice nanofiltration liquid with the content of which the relative molecular weight is lower than 700dal is obtained.
The birch juice nanofiltration solution is used as a raw material, the prepared birch juice nanofiltration solution is subjected to three-stage separation and purification by utilizing a nanofiltration membrane component with 500dal interception molecular weight under the conditions of 0.5-1.5 MPa, 25-45 ℃ and flow rate of 1-4 m/s, after 2h of cyclic nanofiltration separation, intercepting liquid is collected, and birch juice separation liquid with the content relative molecular weight ranging from 500dal to 700dal is obtained.
The polysaccharide content of the birch juice separating liquid prepared by ultrafiltration-nanofiltration three-stage separation concentration process is not less than 10%.
Detailed Description
The present invention is specifically described by the following examples, which are provided for further illustration of the present invention and are not to be construed as limiting the scope of the present invention.
EXAMPLE 1 preparation of birch juice isolate and determination of Total polysaccharide content
1. And (3) preparing a birch juice separating liquid.
Taking about 2000mL of birch juice, carrying out primary separation and purification on the birch juice by using an ultrafiltration membrane component with a molecular weight cut off of 1500dal under the conditions of the pressure of 1.5MPa, the temperature of 35 ℃ and the flow rate of 4m/s, and collecting filtrate after circulating ultrafiltration and separation for 2 hours to obtain the birch juice ultrafiltrate with the content of less than 1500 dal.
The birch juice ultrafiltrate is used as a raw material, a nanofiltration membrane component with 700dal interception molecular weight is utilized to carry out secondary separation and purification on the prepared birch juice ultrafiltrate under the conditions of the pressure of 1.0MPa, the temperature of 35 ℃ and the flow rate of 2m/s, after cyclic nanofiltration separation is carried out for 2 hours, the filtered solution is collected, and the birch juice nanofiltration solution with the content relative molecular weight lower than 700dal is obtained.
The birch juice nanofiltration solution is used as a raw material, the prepared birch juice nanofiltration solution is subjected to three-stage separation and purification by utilizing a nanofiltration membrane component with 500dal interception molecular weight under the conditions of the pressure of 0.5MPa, the temperature of 30 ℃ and the flow rate of 1.5m/s, the nanofiltration separation is carried out for 2 hours, and then the intercepting solution is collected, so that the birch juice separation solution with the content relative molecular weight ranging from 500dal to 700dal is obtained.
2. And (5) measuring the polysaccharide content in the birch juice separating liquid.
The difference between the total sugar mass and the reducing sugar mass in the birch juice is the polysaccharide mass in the birch juice.
Determination of total sugar content in birch juice isolate.
Preparation of control solution:
and (3) drying the glucose standard substance in a drying oven at 60 ℃ for 24 hours, weighing 2.5mg of the glucose standard substance after cooling, placing in a 25mL volumetric flask, adding distilled water for dissolution and dilution to a scale, and shaking uniformly to obtain a glucose control solution of 0.1 mg/mL.
And respectively sucking 0.2, 0.4, 0.6, 0.8 and 1.2mL of glucose control solution into a test tube, supplementing to 2mL with distilled water, respectively adding 1.5mL of 9% phenol solution and 2.5mL of concentrated sulfuric acid, uniformly mixing, cooling to room temperature, measuring the absorbance value at 490nm, and drawing standard curves.
Sucking 0.5mL of the prepared birch juice separating liquid, adding distilled water to make up 2mL, measuring the absorbance value of the sample liquid after the same treatment method is operated, and measuring the total sugar content by referring to a standard curve.
Determination of reducing sugar mass in birch juice isolate.
The determination of reducing sugars was performed by reference to the dinitrosalicylic acid method.
Respectively sucking 0.1mL,0.2mL,0.4mL,0.6mL,0.8mL and 1.2mL of 1mg/mL glucose solution into a test tube, supplementing 2.0mL with distilled water, adding 2.0mL of DNS solution into each tube, placing into a constant temperature water bath at 90 ℃ for 30min, taking out, cooling, diluting to 100mL with distilled water, and respectively measuring absorbance at 550 nm. And drawing a standard curve by taking the concentration of the glucose standard substance as an abscissa and the absorbance value as an ordinate. Sucking 0.5mL of the prepared birch juice separating liquid, adding distilled water to make up 2mL, processing by the same method, measuring the absorbance value of the sample liquid, and measuring the content of the reducing sugar by referring to a standard curve.
Experiments prove that the total sugar content in the birch juice separating liquid prepared per milliliter is 374.28mg, the reducing sugar content is 67.37mg, the polysaccharide content is 306.91mg, and the content of the polysaccharide in the birch juice separating liquid is 36.9%.
EXAMPLE 2 birch sap isolation solution treatment of rat gout experiment
Birch juice isolates were prepared as described in example 1.
The 48 male SD rats were randomly divided into 6 groups of 8. Respectively a model group, a blank group, a positive control allopurinol group and a birch juice separating liquid (high, medium and low dose) group. Allopurinol group was infused with 5ml/kg of allopurinol daily, and birch juice isolation liquid was infused with 18ml/kg, 9ml/kg, 3ml/kg birch juice daily, and the blank group and model group were given equal volumes of physiological saline daily 1 time, 14d continuously, and 7 days, sodium urate crystal injection was injected into knee joints of rats in model group, positive control group, birch juice group (20 g·l -1 ) 300 μl, blank group was injected with an equal volume of physiological saline.
Rat knee swelling degree measurement.
The circumferences of the same parts of the knee joints of the rats are measured respectively at 6, 12, 24 and 36 hours before and after the molding of the rats by using a rope binding method, and the swelling degree of the knee joints of the rats in each group is evaluated according to the swelling degree = the circumference of the knee joint after the molding and the circumference of the knee joint before the molding.
Detection of the content of inflammatory factors TNF-alpha, IL-1 beta and IL-18 in rat serum.
Groups of rats were anesthetized with 10% chloral hydrate on day 14. Blood was drawn from the abdominal aorta into a vacuum collection tube, 60000 r.min -1 Centrifuging for 15min, collecting upper serum, and measuring the content of TNF-alpha, IL-1 beta and IL-18 by enzyme-linked immunosorbent assay.
The experimental results showed that the knee joint of the rats in the model group was significantly swollen compared with the blank group at 6, 12, 24, 36h before and after modeling. Compared with the model group, the joint swelling degree of rats in the allopurinol group and the birch juice separating liquid group is obviously reduced, which indicates that the birch juice separating liquid can effectively reduce the knee joint swelling degree caused by gout and has dose correlation.
Compared with the blank group, the serum of the rats in the model group has obviously increased IL-1 beta, IL-18 and TNF-alpha content. Compared with the model group, the birch juice separating liquid group is obviously lower than the model group in the content of IL-18, TNF-alpha and IL-1 beta, and the result shows that the birch juice separating liquid can effectively inhibit the generation of inflammatory factors of gouty arthritis rats caused by sodium urate, and the effect of the high-dose birch juice separating liquid group is most obvious.
Table 1 extent of knee joint swelling in rats in each group
TABLE 2 Effect on inflammatory factors in rat serum
EXAMPLE 3 comparison of birch sap separation and birch sap stock for reducing blood uric acid
Birch juice isolates were prepared as described in example 1.
36 SPF-grade male rats (160 g.+ -. 10 g) were randomly divided into 4 groups of 9 animals each, and the groups were as follows: blank group; a model group; a birch juice isolate; birch juice stock solution group. Except for the blank group, 1 g/(kg.d) of potassium oxazinate was administered to the remaining 3 groups, and the continuous gastric feeding was performed for 14 days to prepare an animal model of hyperuricemia, after the model preparation was successful, an equal volume of distilled water was administered to the blank group and the model group, 9mg/kg of birch juice was administered daily to the birch juice stock solution group, 9ml/kg of birch juice was administered daily to the birch juice isolate group, and the continuous gastric feeding was performed for 28 days.
Week 3, week 4, and week 5 of the experiment, after 12h of fasting, rat tail blood was taken, serum was isolated, and rat serum UA was measured according to the instructions, after week 6 of the experiment was completed, after 12h of fasting, abdominal aortic blood was taken, centrifuged, serum was isolated, and rat serum UA was measured.
Experimental results show that compared with a blank group, the serum uric acid content of rats in the model group is obviously increased. Compared with the model group, the birch juice stock solution group and the birch juice separating solution group can obviously reduce uric acid level, and the reduction of uric acid content in serum of the birch juice separating solution group is particularly obvious, as shown in table 3.
TABLE 3 Effect of birch sap on serum UA content in hyperuricemia model rats
Claims (6)
1. A birch juice separating liquid, which is characterized in that: the birch juice separating liquid is prepared by ultrafiltration-nanofiltration three-stage separation and concentration process.
2. The birch juice isolate as claimed in claim 1, wherein: the birch juice separating liquid consists of substances with relative molecular weight of 500-700 dal in birch juice.
3. The birch juice isolate as claimed in claim 1, wherein: the total content of polysaccharide in the birch juice separating liquid is not less than 10%, preferably not less than 20%.
4. The birch juice isolate as claimed in claim 1, wherein: the birch juice separating liquid is prepared by a method comprising the following steps:
(1) Under the conditions of pressure of 0.5-1.5 MPa, temperature of 25-45 ℃ and flow speed of 1-4 m/s, carrying out primary separation and purification on the birch juice stock solution by utilizing an ultrafiltration membrane component with molecular weight cut off of 1500dal, and collecting filtered solution after the birch juice stock solution is circularly ultrafiltered and separated for 4h to obtain birch juice ultrafiltrate with content relative molecular weight lower than 1500dal
(2) Taking birch juice ultrafiltrate as raw material, performing secondary concentration and separation on the birch juice ultrafiltrate by using a nanofiltration membrane component with 700dal cut-off molecular weight under the conditions of 0.5-1.5 MPa, 25-45 ℃ and flow rate of 1-4 m/s, and collecting filtrate after cyclic nanofiltration and separation for 3 hours to obtain birch juice nanofiltration liquid with content relative molecular weight lower than 700dal
(3) The birch juice nanofiltration solution is taken as a raw material, the prepared birch juice nanofiltration solution is subjected to three-stage concentration separation by utilizing a nanofiltration membrane component with 500dal interception molecular weight under the conditions of 0.5-1.5 MPa, 25-45 ℃ and flow rate of 1-4 m/s, after 2 hours of cyclic nanofiltration separation, intercepting liquid is collected, and birch juice separation liquid with the content relative molecular weight ranging from 500dal to 700dal is obtained
(4) The polysaccharide content of the birch juice separating liquid prepared by ultrafiltration-nanofiltration three-stage separation concentration process is not less than 10%.
5. The birch juice isolate according to claim 1, which is useful in the treatment of gout disease.
6. The birch juice isolate as claimed in claim 5, which can be used for development of anti-gout food and medicine alone or in combination with other functional components.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111230107.1A CN115998781A (en) | 2021-10-22 | 2021-10-22 | Application of birch juice separating liquid in treating gout |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111230107.1A CN115998781A (en) | 2021-10-22 | 2021-10-22 | Application of birch juice separating liquid in treating gout |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115998781A true CN115998781A (en) | 2023-04-25 |
Family
ID=86027196
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111230107.1A Pending CN115998781A (en) | 2021-10-22 | 2021-10-22 | Application of birch juice separating liquid in treating gout |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115998781A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115998783A (en) * | 2021-10-23 | 2023-04-25 | 北京嘉桦生物技术有限公司 | Birch juice separating liquid for treating chemical liver injury |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107075542A (en) * | 2014-10-31 | 2017-08-18 | 东丽株式会社 | The manufacture method of liquid glucose and xylo-oligosaccharide |
| CN209020199U (en) * | 2018-09-18 | 2019-06-25 | 湖北沙市水处理设备制造厂 | A kind of reverse osmosis unit for birch juice cold press |
| CA3029633A1 (en) * | 2019-01-10 | 2020-07-10 | Jacques Marchildon | Pure water from plant sap |
| WO2021017833A1 (en) * | 2019-07-30 | 2021-02-04 | 浙江养生堂天然药物研究所有限公司 | Anti-aging cosmetic composition |
| CN112754010A (en) * | 2021-01-26 | 2021-05-07 | 呼伦贝尔市林海森林经营管理有限公司 | Preparation method of birch juice concentrated solution |
-
2021
- 2021-10-22 CN CN202111230107.1A patent/CN115998781A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107075542A (en) * | 2014-10-31 | 2017-08-18 | 东丽株式会社 | The manufacture method of liquid glucose and xylo-oligosaccharide |
| CN209020199U (en) * | 2018-09-18 | 2019-06-25 | 湖北沙市水处理设备制造厂 | A kind of reverse osmosis unit for birch juice cold press |
| CA3029633A1 (en) * | 2019-01-10 | 2020-07-10 | Jacques Marchildon | Pure water from plant sap |
| WO2021017833A1 (en) * | 2019-07-30 | 2021-02-04 | 浙江养生堂天然药物研究所有限公司 | Anti-aging cosmetic composition |
| CN112754010A (en) * | 2021-01-26 | 2021-05-07 | 呼伦贝尔市林海森林经营管理有限公司 | Preparation method of birch juice concentrated solution |
Non-Patent Citations (1)
| Title |
|---|
| 魏立群;: "白桦树与桦树皮文化", 大自然, no. 03, pages 55 - 57 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115998783A (en) * | 2021-10-23 | 2023-04-25 | 北京嘉桦生物技术有限公司 | Birch juice separating liquid for treating chemical liver injury |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104877035B (en) | A kind of preparation method of the Blackfungus polyhexose with blood sugar reducing function | |
| CN103923201A (en) | Preparation method of hippocampus active glycoprotein | |
| WO2023124706A1 (en) | Method for preparing milk oligosaccharide, oligosaccharide powder prepared using same, and food | |
| CN109527270A (en) | Leech specific nutrient induces the extracting method of liquid and natural hirudin | |
| CN115998781A (en) | Application of birch juice separating liquid in treating gout | |
| CN106749729B (en) | A kind of Smilacina japonica polysaccharide and its preparation method and application | |
| CN108640342B (en) | Method for separating whey protein from whey wastewater by foam separation method | |
| CN106075436A (en) | A kind of anti-helicobacter pylori oral formulations without antibiotic | |
| CN105177097A (en) | Preparation method and application of lactoferricin for promoting proliferative activity of bone cells | |
| CN104910291B (en) | A kind of jackfruit leaf polyose and its preparation method and application | |
| CN105796587A (en) | Application of bamboo shaving polysaccharides in immunoregulation and tumor resisting | |
| CN100362022C (en) | Cuttlebone polysaccharide CPS-1 and its preparation and use | |
| CN105079143A (en) | Medicine composition for treating nephropathy | |
| CN110404021B (en) | Rhizoma polygonati preparation and preparation method thereof | |
| CN111012863A (en) | Radix ophiopogonis degradation extract and application thereof in preparation of hypoglycemic drugs | |
| CN108771070B (en) | Compound functional beverage prepared by fermenting celery juice with eurotium cristatum, preparation method and application | |
| CN106674365B (en) | A method of small molecule oligosaccharides is isolated and purified from green alga | |
| CN111732672B (en) | Okra polygalacturonic acid with uric acid reducing effect and preparation method and application thereof | |
| CN115998783A (en) | Birch juice separating liquid for treating chemical liver injury | |
| CN113735988B (en) | Extraction method of lentinan | |
| CN113876895B (en) | Preparation method and application of composite extract with hypoglycemic activity | |
| CN112410323B (en) | Preparation method of lamb abomasum chymosin standard | |
| CN115462530B (en) | Chestnut kernel extract, extraction method thereof and application thereof in antioxidant products | |
| CN115414398B (en) | Chestnut extract, preparation method thereof and application thereof in hypoglycemic products | |
| CN114853825B (en) | Method for preparing trehalose from thorn sugar and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |