WO2021017833A1 - Anti-aging cosmetic composition - Google Patents

Abstract

A concentrated birch sap, having a concentration factor of 1.05 - 8 times. The concentrated birch sap has a significant anti-aging effect. The present invention also relates to an anti-aging cosmetic composition comprising the concentrated birch sap. The cosmetic composition exhibits a significant anti-aging effect.

Description

Anti-aging cosmetic composition Technical field

The present invention relates to a concentrated birch sap, and an anti-aging cosmetic composition containing the concentrated birch sap.

Background technique

Birch is a deciduous tree of the birch family. There are currently about 100 species in the world, mainly distributed in the northern temperate zone and the cold temperate zone. Among them, there are about 29 varieties in my country, mainly distributed in the northeast, northwest, north and southwest. Birch trees are mostly grown in remote mountainous areas with little human intervention and no industrial pollution. Birch sap (also called birch sap) is the fresh sap from the birch bark being cut or the trunk is drilled. It is colorless or light yellow, free of precipitation and impurities, and has a light birch fragrance. Birch sap contains a large amount of sugars, amino acids, vitamins, biotin, cytokinins, trace mineral elements, aromatic oils, betulin, saponin and other compounds, which have good anti-aging effects.

Skin aging means that the skin's ability to protect and adjust to the body is reduced, and it cannot adapt to changes in internal and external environments, and changes in overall appearance such as color and shape. It is often manifested as reduced or disappeared skin elasticity, loose skin, increased wrinkles, and dry skin , Thinning, easy desquamation, itching, dull complexion, dull, and accompanied by stains and warts, affecting appearance and confidence. With the increasingly serious problem of population aging and the continuous improvement of living standards, consumers' anti-wrinkle awareness continues to increase, and more and more consumers are eager to use cosmetics to maintain their youthful appearance. According to statistics, anti-aging cosmetics have become the category with the fastest growth rate and greatest potential in the Chinese cosmetics market.

Summary of the invention

In one aspect, the present invention provides a concentrated birch sap with a concentration factor of about 1.05-8 times, preferably about 1.1-4 times, and more preferably about 1.2-2 times. The concentrated birch sap has a significant anti-aging effect.

The birch sap involved in the present invention is obtained from the genus Betula, Betula alba, Betula pubescens, Betula pendula and Asian white birch (Betula platyphylla). Varieties. The birch sap is a colorless, transparent, no-sediment-free, and no-drug sap that is artificially collected by drilling holes at the base of the trunk of the birch from thawing to early spring. The birch sap is commercially available and used as it is, for example, it can be purchased from Daxinganling Chaoyue Wild Berry Development Co., Ltd.

The concentrated birch sap in the present invention is obtained by concentrating the above-mentioned commercial products. Concentration methods are known in the art, such as heating concentration, low-temperature vacuum concentration, membrane concentration and the like. In the present invention, the concentration is preferably carried out by a low-temperature freeze concentration or membrane concentration process. For example, the commercially available birch juice stock solution is input into a low-temperature drying equipment, the temperature is lowered to -40°C to -70°C, and the vacuum is applied to 0.1-30Pa. Concentrated in vacuum at low temperature to obtain concentrated birch sap with different concentration times.

Unexpectedly, the present inventors found that, compared with the unconcentrated birch tree sap, the concentrated birch sap has significantly better moisturizing and anti-aging effects, and it can promote collagen I, collagen in the extracellular matrix. The synthesis of III, IV, VII, XVII, elastin, laminin, etc., reduce the expression of metal matrix protease MMP-1, promote the production of hyaluronic acid, and strengthen the structural integrity and functionality of the epidermis, dermis, and dermal-epidermal junctions , Improve skin elasticity, reduce wrinkles, improve skin barrier, reduce transdermal water loss, anti-inflammatory and soothing effects.

Furthermore, the inventors also found that the anti-aging effect of concentrated birch sap is not a simple linear relationship with its degree of concentration, but a trend that first increases and then decreases as the concentration of birch sap increases, that is, when the concentration of birch sap increases When it reaches a certain level, the concentrated birch sap will inhibit cell proliferation and viability to a certain extent, and inhibit the expression of aging-related genes inside and outside the cell. Therefore, controlling the concentration ratio of birch sap is key. In the present invention, controlling the concentration ratio of birch sap is about 1.05-8 times, preferably about 1.1-4 times, more preferably about 1.2-2 times.

In another aspect, the present invention relates to the use of the concentrated birch sap in an anti-aging cosmetic composition.

In another aspect, the present invention also relates to an anti-aging cosmetic composition, which comprises (A) the concentrated birch sap.

In a preferred embodiment, the anti-aging cosmetic composition does not contain chelating agents such as EDTA salt, sodium polyphosphate, sodium metaphosphate, and gluconic acid.

The anti-aging cosmetic composition does not contain any added water, but does not exclude the moisture inherently contained in each component.

The content of the concentrated birch sap is about 18-98% by weight, preferably 20-95% by weight, more preferably 22-90% by weight, most preferably 30-90% by weight, based on the total weight of the anti-aging cosmetic composition .

In addition to the concentrated birch juice of the component (A), the anti-aging cosmetic composition may optionally contain (B) ingredients commonly used in skin care cosmetic compositions, including vehicles, active ingredients and auxiliary materials, etc. . Component (B) is known in the art, and those skilled in the art can select its type and amount according to needs. For example, the content of component (B) is about 2-82% by weight, based on the anti-aging cosmetic composition The total weight.

The vehicle includes, for example, a diluent, a dispersant, or a carrier, and examples thereof include, but are not limited to, ethanol, dipropylene glycol, butylene glycol, and the like. The content of the vehicle in the cosmetic composition is known in the art, for example, it usually accounts for 0.5-20% of the total weight of component (B).

The active ingredients include, for example, emollients, moisturizers, anti-aging active ingredients and the like.

Examples of the emollient include, but are not limited to, olive oil, macadamia nut oil, sweet almond oil, grape seed oil, avocado oil, corn oil, sesame oil, soybean oil, peanut oil, white flower seed oil, safflower seed oil, Dogtooth rose hip oil, argan tree kernel oil, jojoba seed oil, sunflower seed oil, palm tree fruit oil, squalane, ethylhexyl palmitate, isopropyl myristate, hydrogenated polyisobutylene, isotene Hexane, isododecane, diethylhexyl carbonate, dioctyl carbonate, isopropyl lauroyl sarcosine, isononyl isononanoate, hydrogenated polydecene, glycerol tri(ethylhexanoate) , Cetyl alcohol ethyl hexanoate, bis-diethoxy diethylene glycol cyclohexane 1,4-dicarboxylate, caprylic/capric triglyceride, oleyl erucate, octyldodecanol Myristate, Octyldodecanol, Dimethicone, Octyl Dimethicone, Cetyl Dimethicone, Cyclopenta Dimethicone, etc. One or more. Examples of solid emollients include, but are not limited to, cetyl alcohol, stearyl alcohol, cetearyl alcohol, behenyl alcohol, scylitol, lauric acid, myristic acid, palmitic acid, stearic acid, beeswax, candelilla Tree wax, carnauba wax, lanolin, ozokerite, jojoba seed wax, paraffin wax, microcrystalline wax, hydrogenated rice bran wax, hydrogenated coconut oil glycerides, glyceryl behenate/eicosanate, myristyl alcohol One or more of myristate, bis-diglyceride polyacyl adipate-2, shea butter, and muluxing palm seed butter. The content of the emollient in the cosmetic composition is known in the art, for example, it usually accounts for 1-50% of the total weight of component (B).

Examples of the moisturizer include, but are not limited to, glycerin, diglycerin, butylene glycol, propylene glycol, 1,3-propanediol, dipropylene glycol, 1,2-pentanediol, polyethylene glycol-8, polyethylene glycol Alcohol-32, methylglucitol-10, methylglucitol-20, PEG/PPG-17/6 copolymer, glycerol-7, glycerol-26, glycerol glucoside, PPG-10 methyl glucose ether, PPG-20 methyl glucose ether, PEG/PPG/polybutylene glycol-8/5/3 glycerin, sucrose, trehalose, rhamnose, mannose, raffinose, Betaine, erythritol, xylitol, urea, glyceryl polyether-5 lactate, sodium hyaluronate, hydrolyzed sodium hyaluronate, acetylated sodium hyaluronate, sodium polyglutamate, Sclerotium hydrolysate One or more of gum, short-stemming enzyme polysaccharide, tremella polysaccharide, caper seed polysaccharide, etc. The content of the moisturizer in the cosmetic composition is known in the art, for example, it usually accounts for 1-30% of the total weight of the component (B).

Examples of the anti-aging active ingredients include, but are not limited to, tocopherol (vitamin E), retinol, retinol palmitate, hydrolyzed collagen, hydrolyzed elastin, allantoin, yeast extract, oryzanol, tetrahydroturmeric Ellagic acid, ubiquinone, whey protein, acetyl hexapeptide-8, palmitoyl pentapeptide-4, salicyl sphingosine, birch sap concentrate, silymarin, silk protein, tocopherol phosphate Sodium, ribonucleic acid (RNA), dipeptide diaminobutyrylbenzylamide diacetate, palmitoyl tripeptide-5, oligopeptide-1, hexapeptide-9, palmitoyl oligopeptide, palmitoyl tetrapeptide-7 , Grape (VITIS VINIFERA) seed extract, PTEROCARPUS (MARSUPIUM) bark extract, tea (CAMELLIA SINENSIS) polyphenols, wine extract, apple seed extract, FAGUS SYLVATICA bud extract, Hydrolyzed baobab (ADANSONIA DIGITATA) extract, Artemia (ARTEMIA) extract, IRIS FLORENTINA root extract, Hesperidin, Ginsenoside, Salvia miltiorrhiza extract, Nicotinamide, Bearberry Acid, sodium hyaluronate, sodium acetylated hyaluronate, hydrolyzed sodium hyaluronate, lycopene, coffee (COFFEA ARABICA) extract, dipeptide-2, lactic acid, superoxide dismutase (SOD), moon see OENOTHERA BIENNIS oil, ceramide, dipalmitoyl hydroxyproline, hydroxystearic acid, salicylic acid, ergothioneine, lysolecithin, carnosine, decarboxylated carnosine HCL, lipoic acid, adenosine, glycogen, One or more of resveratrol, ferulic acid, lysate of fermentation product of difid yeast, lysate of fermentation of lactic acid bacteria, etc. The content of the anti-aging active ingredient in the cosmetic composition is known in the art, for example, it usually accounts for 0.01-10% of the total weight of component (B).

The auxiliary materials include, for example, emulsifiers, thickeners, preservatives, perfumes and the like.

Examples of the emulsifier include, but are not limited to, cetearyl oleate, sorbitan oleate, polysorbate-60, polysorbate-80, methylglucose sesquistearic acid Ester, PEG-20 methyl glucose sesquistearate, PEG-40 hydrogenated castor oil, PPG-26-butanol-26, PEG-4 polyglycerol-2 stearate, PEG-60 hydrogenated Castor oil, steareth-2, steareth-21, PPG-13-decyltetradeceth-24, cetearyl glucoside, PEG-100 stearate, glycerin Stearate, Glyceryl Stearate SE, Coco Glucoside, Ceteareth-25, PEG-40 Stearate, Polyglyceryl-3 Methyl Glucose Distearate, Glyceryl stearate citrate, polyglyceryl-10 stearate, polyglyceryl-10 myristate, polyglyceryl-10 dioleate, polyglyceryl-10 laurate, polyglyceryl-10 isostearate Fatty acid ester, polyglyceryl-10 oleate, polyglyceryl-10 diisostearate, polyglyceryl-6 laurate, polyglyceryl-6 myristate, sucrose stearate, sucrose polystearate One or more of acid esters. The content of the emulsifier in the cosmetic composition is known in the art, for example, it usually accounts for 0.5-10% of the total weight of component (B).

Examples of the thickener include, but are not limited to, carbomers, acrylic acid (ester) and its derivatives, xanthan gum, gum arabic, polyethylene glycol-14M, polyethylene glycol-90M, succinyl poly One or more of high molecular polymers such as sugar, hydroxyethyl cellulose, hydroxypropyl cellulose, and hydroxypropyl methyl cellulose. The content of the thickener in the cosmetic composition is known in the art, for example, it usually accounts for 0.1-10% of the total weight of the component (B).

Examples of the preservatives include, but are not limited to, methylparaben, propylparaben, phenoxyethanol, benzyl alcohol, phenethyl alcohol, bis(hydroxymethyl)imidazolidinylurea, potassium sorbate, sodium benzoate, chlorobenzene Glycerol, sodium dehydroacetate, caprylic hydroxamic acid, 1,2-hexanediol, 1,2-pentanediol, p-hydroxyacetophenone, caprylyl glycol, glyceryl caprylate, undecylenic acid One or more of glycerides, sorbitan caprylate, ethylhexylglycerol, tree peony root extract, etc. The content of the preservative in the cosmetic composition is known in the art, for example, it usually accounts for 0.01-2% of the total weight of the component (B).

The anti-aging cosmetic composition of the present invention can be prepared by any suitable method known in the art. For example, it can be prepared by dissolving tanks, emulsifying pots, dispersers, and delivery pumps commonly used in the cosmetics field. When preparing, put the water-soluble substance into the water-phase dissolving kettle, and the oil-soluble substance into the oil-phase dissolving kettle, and heat the temperature of the two kettles to about 80°C. For the raw materials that are easy to agglomerate, they can be mixed with a disperser first. Pre-dispersion. After the dissolution is completed, the oil phase and the water phase are transported to the emulsifying pot for homogenization and emulsification for about 5-15 minutes. After the emulsification is completed, the temperature of the material body is reduced to normal temperature, optional flavors, preservatives, etc. are added, and the pH of the product is adjusted as necessary. The products can be filled and shipped only after the relevant test indicators are qualified.

The above preparation methods can be deleted or adjusted according to dosage form requirements. According to needs, the anti-aging cosmetic composition can be made into various dosage forms such as creams, creams, lotions, and essences.

Example

Hereinafter, the present invention will be further described in detail with reference to the embodiments. However, it should be understood that these examples and comparative examples are only used to describe the present invention in more detail, and should not be understood as limiting the scope of the appended claims of the present invention in any form.

Example 1 : The effect of birch sap stock and concentrated birch sap on the expression of senescence-related genes

In this example, the effects of the original birch sap and the concentrated birch sap with different concentration times on the expression of senescence-related genes were tested and compared.

1. Concentration of birch sap

The fresh white birch sap stock purchased from Daxinganling Chaoyue Wild Berry Development Co., Ltd. is fed into low-temperature drying equipment, cooled to -65℃, vacuumed to 0.1Pa, and concentrated to 1.05 times, 1.1 times, 1.2 times, 1.5 times, and 2 Times, 4 times, 8 times.

2. Test

Experimental instruments: fluorescence quantitative PCR instrument (Roche), ultra-clean workbench (Su Jing), carbon dioxide incubator (Binder), microplate reader (BIO-TEK), micro oscillator.

Experimental reagents and consumables: human primary fibroblasts, 6-well plates, fibroblast culture medium, RNA extraction kit, reverse transcription kit, Trizol lysis solution, etc.

The steps of gene expression analysis based on fibroblasts are as follows:

(1) Inoculation: Inoculate cells into a 6-well plate at a seeding density of 5E5/well, and incubate overnight in a 37°C and 5% CO ₂ incubator;

(2) Administration: When the plating rate of cells in the 6-well plate reaches about 60%, add the test substance of each group, and each group has 6 multiple wells;

(3) Sample collection: After 24h in a 37°C and 5% CO ₂ incubator, discard the culture solution, add 1mL Trizol to each well, pipette to lyse the cells, and collect the sample;

(4) PCR detection: RNA is extracted, reverse transcribed to cDNA, and then subjected to fluorescence quantitative PCR detection;

(5) Analysis: The 2- ^△△CT method is used for calculation of the results, and the T-Test method is used for statistical analysis.

The test results are shown in the table below.

sample	Collagen I	Collagen III	Collagen IV	Collagen VII	Elastin	Laminin
Birch sap	3.156±0.239	1.378±0.276	1.259±0.051	1.223±0.109	4.822±0.462	1.059±0.151
1.05 times concentrated	4.453±0.565*	1.461±0.268	1.317±0.126	1.305±0.112	5.055±0.541	1.106±0.092
1.1 times concentrated	8.655±0.778**	2.791±0.231**	1.696±0.183*	2.172±0.187**	8.913±0.812**	1.685±0.147**
1.2 times concentrated	11.031±1.167**	3.913±0.344**	2.568±0.235**	1.847±0.193**	10.313±1.347**	2.013±0.171**
1.5 times concentrated	10.588±1.012**	3.855±0.362**	2.383±0.246**	2.106±0.212**	10.156±1.308**	1.986±0.163**
Concentrated 2 times	11.096±0.985**	3.028±0.353**	2.425±0.229**	1.965±0.186**	10.065±1.281**	1.891±0.194**
Concentrated 4 times	9.855±1.056**	2.829±0.301**	2.181±0.202**	1.718±0.279*	8.851±0.973**	1.665±0.159**
8 times concentrated	4.766±0.852*	1.553±0.218	1.331±0.117	1.263±0.148	5.131±0.499	1.117±0.102

Note: * indicates that it is significant compared with the original solution, and the P value is less than 0.05; ** indicates that it is extremely significant compared to the original solution, and the P value is less than 0.01.

The above results show that compared with the original birch sap, the birch sap with a concentration of 1.1-4 times significantly increases the expression of senescence-related genes in basal fibroblasts, especially when the concentration is 1.2-2 times The gene expression levels of collagen I, collagen III, collagen IV, collagen VII, elastin, and laminin are all significantly higher than that of birch sap.

Example 2 : The effect of birch sap stock and concentrated birch sap on the expression of senescence-related proteins

In this example, the effects of birch sap stock and different concentration of birch sap on the expression of senescence-related proteins were tested and compared.

1. Concentration of birch sap

The fresh white birch sap stock purchased from Daxinganling Chaoyue Wild Berry Development Co., Ltd. is fed into low-temperature drying equipment, cooled to -65℃, vacuumed to 0.1Pa, and concentrated to 1.05 times, 1.1 times, 1.2 times, 1.5 times, and 2 Times, 4 times, 8 times.

2. Test

Experimental equipment: ultra-clean workbench (Su Jing), plate washer (BIO-RAD), microplate reader (BIO-TEK), carbon dioxide incubator (Binder)

Experimental reagents and consumables: human primary fibroblasts, 12-well plates, fibroblast culture medium, ELISA test kits with different indicators, etc.

The test steps are as follows:

(1) Inoculation: Inoculate in a 12-well culture plate at an inoculation density of 2E5/well, culture in a 37°C and 5% CO2 incubator, and change the medium every two days;

(2) Dosing: When the cell fusion reaches more than 60% again, add different groups of test substances, each group has 6 multiple holes;

(3) Receiving samples: After 48 hours in a 37°C and 5% CO ₂ incubator, discard the culture solution, add 1 mL Trizol to each well, pipette to lyse the cells, and collect the samples;

(4) Detection: Perform index determination according to the determination method of ELISA kit;

(5) Analysis: T-Test method is used for statistical analysis.

The test results are shown in the table below.

sample	Collagen I (ng)	Collagen III (ng)	Collagen IV (ng)	Collagen VII (ng)	Elastin (ng)	Laminin (ng)
Birch sap	118.209±9.242	111.745±8.861	15.048±1.297	11.215±1.431	2.892±0.217	1.286±0.161
1.05 times concentrated	121.126±9.817	114.236±9.250	15.721±1.332	12.736±1.038	3.011±0.319	1.317±0.141
1.1 times concentrated	148.105±8.275*	129.434±6.117*	19.156±1.676*	18.245±1.993**	4.833±0.473**	2.518±0.222**
1.2 times concentrated	176.156±9.239**	159.023±10.781**	21.312±2.093**	18.016±2.082**	5.022±0.515**	2.566±0.248**
1.5 times concentrated	168.007±10.641**	151.279±10.391**	20.298±2.163**	16.719±1.478**	4.913±0.483**	2.609±0.216**
Concentrated 2 times	156.119±10.393**	143.209±7.191**	19.886±0.979**	17.914±1.838**	4.706±0.412**	2.892±0.237**
Concentrated 4 times	137.263±7.088*	131.783±8.231*	18.716±1.751*	16.413±1.359*	4.318±0.392**	2.288±0.216**
8 times concentrated	127.135±9.943	113.135±9.545	15.643±1.581	11.617±1.011	2.984±0.218	1.253±0.117

Note: * indicates that it is significant compared with the original solution, and the P value is less than 0.05; ** indicates that it is extremely significant compared to the original solution, and the P value is less than 0.01.

The above results show that compared with the original birch sap, birch sap with a concentration of 1.1-4 times can significantly increase the expression of basal fibroblasts and senescence-related proteins, especially when the concentration is 1.2-2 times , Collagen I, Collagen III, Collagen IV, Collagen VII, elastin, and laminin protein expression levels are all significantly higher than that of birch sap.

Example 3 : The effect of birch sap stock and concentrated birch sap on tissues (reconstituted 3D full-thickness skin model)

In this example, the effects of the original birch sap and the birch sap with different concentration times on the reconstructed 3D full-thickness skin model were tested and compared.

1. Concentration of birch sap

The fresh birch sap stock solution purchased from Daxinganling Chaoyue Wild Berry Development Co., Ltd. is fed into the reverse osmosis circulation device, the operating pressure is controlled at 0.5-5bar, and the operating temperature is controlled at 20-35℃, and circulated until the birch sap is respectively concentrated to 1.1 Times, 1.2 times, 1.5 times, 2 times, 4 times.

2. Test method

Experimental equipment: ultra-clean workbench (Su Jing), carbon dioxide incubator (Binder), UVA and UVB irradiator, microplate reader (BIO-TEK), cryostat (Leica).

Experimental reagents and consumables: 3D full-thickness skin model (made in the laboratory), ELISA test kits with different indicators, MTT (3-(4,5-dimethylthiazole-2)-2,5-diphenyltetrazolium Azole bromide) kits and the like.

The test steps are as follows:

(1) 3D full-thickness model construction: use keratinocytes and fibroblasts to construct a 3D skin model;

(2) Modeling and drug administration: When the model leaves the factory, that is, day 0, SSUV irradiation treatment (UVA: 30J/cm2; UVB: 50mJ/cm2) is carried out every day, and then a certain amount of samples are respectively coated on the corresponding model surface , The model control group is only coated with the model culture medium, each group has 6 multiple holes, and the total duration of action is 4 days;

(3) Sampling and testing: After the sample is over, collect the culture medium and use the ELISA kit to determine the MMP-1 (matrix metalloproteinase 1). In addition, take 3 models from each group and use the MTT kit to test the cell proliferation viability. MTT value, the remaining 3 were tissue fixed, and IHC staining was used to determine the content of collagen IV at the junction of true epidermis.

(4) Analysis: T-Test method is used for statistical analysis.

The test results are shown in the table below.

Note: * indicates that it is significant compared with the original solution, and the P value is less than 0.05; ** indicates that it is extremely significant compared to the original solution, and the P value is less than 0.01.

The MTT value test results show that compared with the original birch sap, the birch sap with a concentration of 1.1-4 times can significantly improve the tissue viability of the reconstructed 3D full-thickness skin model. Similarly, the relative expression of collagen IV in the true epidermal junction of the 3D skin model using birch sap with a concentration of 1.1-4 times was significantly higher than that of the birch sap stock. The MMP-1 results showed that the 1.1-4 times concentrated birch sap was significantly lower than the original birch sap. The above results also show that when the birch sap concentration is 1.1-2, it can significantly improve the tissue viability of the recombinant 3D full-thickness skin model, promote the relative expression of collagen IV at the junction of the true epidermis, and reduce the extracellular matrix. The expression of MMP-1.

Example 4 : Preparation of anti-aging cream composition

The formula of the anti-aging cream composition is shown in the following table:

The aforementioned anti-aging cream composition is prepared as follows:

1. Oil phase: add 4, 6, 7, 8, 9, 11, 12, 13, 15, 21 raw materials into the oil phase pot, and heat to 80°C, dissolve and mix well;

2. Mix No. 2, 16 and 18 raw materials evenly at room temperature;

3. Mix the raw materials such as No. 10, 14, 17, and 20 at room temperature evenly;

4. Water phase: Heat 1, 3, and 5 raw materials to 80°C, add the mixture of step 2, dissolve, and mix well;

5. Emulsification: add the water phase and the oil phase to the emulsification tank, keep it at 80°C, homogenize emulsification at 3000rpm for 5 minutes, add No. 19 raw material after emulsification is complete;

6. The mixture of step 3 is added when the temperature is lowered to 40°C by stirring, and the mixture is uniformly stirred and then discharged to obtain the anti-aging cream composition.

In this example, the effect of the above-mentioned anti-aging cream composition on the reconstructed 3D full-thickness skin model was tested.

As a result, it was found that the anti-aging cream composition can significantly improve the tissue viability of the recombinant 3D full-thickness skin model, promote the relative expression of collagen IV at the real epidermal junction, and reduce the expression of MMP-1 in the extracellular matrix. .

Example 5 : Preparation of anti-aging eye cream composition

The formula of the anti-aging eye cream composition is shown in the following table:

Serial number	raw material	weight%
1	2 times concentrated white birch (BETULA ALBA) sap	70.75
2	glycerin	6.00
3	Triglyceride (ethylhexanoate)	3.00
4	Butanediol	5.00
5	Polydimethylsiloxane	2.00
6	C12-20 Alkyl Glucoside, C14-22 Alcohol	2.00
7	Cetyl alcohol	2.00
8	Creatine	2.00
9	Phenyl trimethicone	1.00
10	Hexapeptide-9	3.00
11	Glyceryl stearate, PEG-100 stearate	0.50
12	Phenoxyethanol	0.50
13	Panthenol	0.30
14	Dipeptide diaminobutyrylbenzylamide diacetate	1.00
15	Arginine	0.15
16	Carbomer	0.25
17	Sodium acetylated hyaluronate	0.10
18	Methyl paraben	0.10
19	Allantoin	0.15
20	Sodium polyglutamate	0.20

The aforementioned anti-aging eye cream composition is prepared as follows:

1. Oil phase: Add 3, 5, 6, 7, 9, 11 raw materials into the oil phase pot, and heat to 80°C, dissolve, and mix well;

2. Mix No. 2 and No. 16 materials evenly at room temperature;

3. Mix the raw materials such as No. 10, 14, and 17 materials uniformly at room temperature;

4. Water phase: Heat 1, 4, 8, 13, 18, 19, and 20 raw materials to 80°C, add the mixture of step 2, dissolve, and mix well;

5. Emulsification: add the water phase and the oil phase to the emulsification tank, keep the temperature at 80°C, homogenize emulsification at 3000rpm for 5 minutes, add the 12 and 15 raw materials after the emulsification is completed;

6. Add the mixture of step 3 when stirring and cooling down to 40°C, stir evenly and then discharge to obtain the anti-aging eye cream composition.

Using the half-face control test method, 20 volunteers were tested as follows before using the product and 4 weeks after using the product:

1) Use primos to take pictures of the volunteers' left and right outer corners of the eyes, and use software to calculate wrinkle parameters, including wrinkle number, wrinkle area, wrinkle depth, etc.; and

2) Use Corneometer to test the skin moisture content of the left and right corners of the eyes.

The results showed that 18 of the 20 subjects had a significant increase in water content in the skin of the corners of the eyes, and 15 of them were significantly lighter and lighter, the area of wrinkles decreased, and the number of wrinkles decreased.

Example 6 : Anti-aging emulsion composition

The formula of the anti-aging emulsion composition is shown in the following table:

To	raw material	weight%
1	1.1 times concentrated white birch (BETULA ALBA) sap	67.55
2	glycerin	6.00
3	Dipropylene glycol	5.00
4	Squalane	5.00
5	Avocado (BUTYROSPERMUM PARKII) fruit butter	2.00
6	Synthetic spermaceti	1.00
7	Isododecane	2.00
8	Xanthan gum	0.10
9	Acetyl hexapeptide-8	8.50
10	Sodium acetylated hyaluronate	0.05
11	Acrylic (ester)/C10-30 alkanol acrylate cross-linked polymer	0.10
12	PEG-100 stearate, glyceryl stearate	1.00
13	C14-22 alcohol, C12-20 alkyl glucoside	1.00
14	Methyl paraben	0.20
15	Arginine	0.10
16	Phenoxyethanol	0.40

The anti-aging emulsion composition described above is prepared as follows:

1. Oil phase: add No. 4, 5, 6, 7, 12, 13 raw materials into the oil phase pot, and heat to 80°C, dissolve, and mix well;

2. Mix No. 2, 8, and 11 materials evenly at room temperature;

3. Mix the raw materials such as No. 9, 10, 16 at room temperature evenly;

4. Water phase: Heat 1, 3, and 14 raw materials to 80°C, add the mixture of step 2, dissolve, and mix well;

5. Emulsification: add the water phase and the oil phase to the emulsification tank, keep the temperature at 80°C, homogenize and emulsify for 5 minutes at 3000 rpm, and add No. 15 raw material after the emulsification is completed;

6. Add the mixture of step 3 when stirring and cooling down to 40° C., stir evenly and then discharge to obtain the anti-aging emulsion composition.

Twenty subjects used the emulsion of the above formula for 4 weeks and subjectively evaluated the use. As a result, 18 people reported a significant increase in skin moisture content, and their skin was smooth, tender and elastic. Among them, 16 people reported significant improvement in nasolabial folds and eye wrinkles.

Example 7 : Anti-aging essence composition

The formula of the anti-aging essence composition is shown in the following table:

To	raw material	weight%
1	1.5 times concentrated white birch (BETULA ALBA) sap	73.00
2	PPG-10 Methyl Glucose Ether	4.00
3	PEG/PPG-17/6 copolymer	2.00
4	Trehalose	2.00
5	glycerin	6.00
6	1,3-propanediol	5.00
7	Palmitoyl tripeptide-5	2.50
8	Dipeptide diaminobutyrylbenzylamide diacetate	1.30
9	Sodium acetylated hyaluronate	0.30
10	Acrylic (ester)/C10-30 alkanol acrylate cross-linked polymer	0.20
11	Xanthan gum	0.10
12	Panthenol	0.50
13	Methyl paraben	0.15
14	Arginine	0.35
15	Artemia (ARTEMIA) extract	2.20
16	Phenoxyethanol	0.40

The aforementioned anti-aging essence composition is prepared as follows:

1. Add raw materials No. 1, 2, 3, 4, 6, 12, and 13 to the emulsification tank and keep it at 80℃;

2. Mix No. 5, 10 and 11 raw materials evenly at room temperature;

3. Mix No. 7, 8, and 9 raw materials evenly at room temperature;

4. Add No. 14 raw material to adjust the pH value;

5. Add No. 15, No. 16 and step 3 raw materials when stirring and cooling down to 40°C, mix well and discharge, to obtain an anti-aging essence composition.

Using the half-face control test method, 20 volunteers were tested as follows before using the product and 8 weeks after using the product:

1) Use primos to take pictures of the volunteers' left and right outer corners of the eyes, and use software to calculate wrinkle parameters, including wrinkle number, wrinkle area, wrinkle depth, etc.;

2) Use Corneometer to test the skin moisture content of the left and right corners of the eyes; and

3) Test the transcutaneous water loss rate (TEWL value) of the left and right cheeks with Tewameter.

The results showed that 18 of the 20 subjects had a significant increase in water content in the skin of the corners of the eyes, and 15 of them had lighter and lighter eye corners, the area of wrinkles decreased, and the number of wrinkles decreased. Among the 20 subjects, 18 had a significant increase in skin moisture content and a significant decrease in TEWL value. Among them, 16 of the subjects became significantly lighter and lighter than nasolabial lines and eye wrinkles, wrinkle area decreased, and number of wrinkles decreased.

The technical solutions of the above-mentioned embodiments are preferred implementations of the present invention. Several improvements and changes can be made without departing from the principle of the present invention, and these improvements and changes should also be regarded as falling within the protection scope of the present invention.

Claims (6)

1. A concentrated birch sap with a concentration ratio of 1.05-8 times, preferably 1.1-4 times, more preferably 1.2-2 times.
2. The use of concentrated birch sap in an anti-aging cosmetic composition, wherein the concentration ratio of the concentrated birch sap is 1.05-8 times, preferably 1.1-4 times, more preferably 1.2-2 times.
3. The use according to claim 2, wherein the anti-aging cosmetic composition does not contain any added water.
4. An anti-aging cosmetic composition comprising concentrated birch sap, wherein the concentration multiple of the concentrated birch sap is 1.05-8 times, preferably 1.1-4 times, more preferably 1.2-2 times.
5. The anti-aging cosmetic composition of claim 4, which comprises 18-98% by weight, preferably 20-95% by weight, more preferably 22-90% by weight, most preferably 30-90% by weight of concentrated birch sap based on The total weight of the anti-aging cosmetic composition.
6. The anti-aging cosmetic composition according to claim 4 or 5, wherein the anti-aging cosmetic composition does not contain any added water.