CN113332187B

CN113332187B - Permeation-promoting composition containing thermal spring water and application thereof

Info

Publication number: CN113332187B
Application number: CN202110634289.2A
Authority: CN
Inventors: 谢茹玉; 袁裕泉; 张娇; 韩志东
Original assignee: Quanhou Guangzhou Research Institute Of Biotechnology Co ltd
Current assignee: Quanhou Guangzhou Research Institute Of Biotechnology Co ltd
Priority date: 2021-06-07
Filing date: 2021-06-07
Publication date: 2022-04-08
Anticipated expiration: 2041-06-07
Also published as: CN113332187A

Abstract

The invention provides a permeation-promoting composition containing hot spring water, which comprises the following components: hot spring water, propylene glycol, PEG/PPG/polytetramethylene glycol-8/5/3 glycerol, schizosaccharomyces cerevisiae fermentation product lysate, piperine. The invention provides a permeation-promoting composition with a plurality of permeation-promoting components by adopting natural active substances and combining water-soluble and oil-soluble permeation-promoting raw materials according to a specific proportion. The piperine, the hot spring water and the lysate of the yeast fermentation product in the composition can improve the diffusion speed of the functional components in the skin; the PEG/PPG/polytetramethylene glycol-8/5/3 glycerin and the polyhydric alcohol in the composition can increase the distribution of the functional components to the skin, and the compounding of the components can make the components exert a synergistic effect, thereby avoiding the problems that the permeation promoting effect cannot be balanced and the irritation is easily caused by the use amount of a single permeation promoting path.

Description

Permeation-promoting composition containing thermal spring water and application thereof

Technical Field

The invention relates to the technical field of cosmetics, in particular to a permeation-promoting composition containing hot spring water and application thereof.

Background

Transdermal absorption of cosmetics refers to a process in which functional ingredients in cosmetics act on the skin surface or enter different skin layers such as epidermis or dermis depending on the effectiveness of the product, and accumulate and act at the site.

The transdermal absorption of cosmetics is very important and is a key point affecting whether cosmetics are effective or not. The more effective components absorbed by the skin, the more remarkable the effect.

It is generally believed that the primary barrier to transdermal penetration is from the stratum corneum. The diffusion pathways in the stratum corneum are both intercellular diffusion and cell membrane diffusion. It is generally accepted that lipid-soluble, non-polar materials diffuse readily through the intercellular lipid bilayer, while water-soluble and polar materials diffuse readily through keratinocytes.

There are various ways of influencing the transdermal absorption of the active ingredients on the skin, mainly including the following:

1. the skin structure is changed, and the diffusivity of the effective components in the skin is improved. The theory suggests that after the penetration enhancer enters the skin, the lipid-like structure in the stratum corneum is destroyed, the ordered stack structure of the flat keratinocytes is changed, the order of lipid arrangement is reduced, and the lipid is completely fluidized, so that the functional components are promoted to pass through smoothly.

2. Hydration technology, when the skin absorbs a large amount of water and is fully hydrated, the stratum corneum can expand into a porous state, the barrier function is reduced, and thus the penetration of substances is facilitated; skin hydration generally facilitates transdermal absorption. Since the cells themselves swell when the hydration force of nitrogen-containing substances in the keratin of the cells of the stratum corneum is increased, the degree of compaction of the structure is reduced and the permeability of the substance is increased. In this case, water-soluble and polar substances are more easily permeated from the stratum corneum cells. Therefore, moisturizers are often added to cosmetics to improve the absorption of the active ingredients.

3. The penetration enhancer is the most common penetration enhancing method applied so far, and the penetration enhancer for percutaneous absorption helps functional components to overcome the barrier of the skin stratum corneum and reversibly change the barrier function of the skin stratum corneum without damaging any active cells, so that the aim of increasing the solubility of the drug in the skin is fulfilled, and the penetration rate of the drug is increased.

Patent CN109172430A discloses a permeation-promoting composition containing small molecular group water, which comprises 0.1-3 parts of bis-diethoxydiethylene glycol cyclohexane 1, 4-dicarboxylate, 3-21 parts of seawater, 0.3-2 parts of sodium bis (lauramide glutamine) lysine and 0.1-1 part of hydrogenated lecithin. Wherein, the bis-diethoxydiol cyclohexane 1, 4-dicarboxylate interacts with small molecular group water to improve the permeability of skin by changing the arrangement of skin stratum corneum cells.

Patent CN102579276B discloses a penetration-promoting composition for cosmetics, which comprises amino acid humectant, sodium hyaluronate, nicotinamide, allantoin and deionized water as main ingredients. The composition of the invention allows the stratum corneum to expand into a porous state by hydration, thereby facilitating penetration of the substance.

At present, in the field of cosmetics, transdermal penetration enhancers mainly comprise polyhydric alcohols, lipids, moisturizers and transdermal enhancement peptides, but most of the attention of the existing publications is concerned about promoting the absorption of water-soluble active substances, and the existing penetration enhancing modes are single, so that the penetration enhancing effect of the product cannot be increased through multiple ways.

Disclosure of Invention

The invention aims to overcome the defects in the prior art and provides a permeation-promoting composition containing hot spring water and application thereof.

In order to achieve the purpose, the invention adopts the technical scheme that:

the invention provides a permeation-promoting composition containing hot spring water, which comprises the following components: hot spring water, propylene glycol, PEG/PPG/polytetramethylene glycol-8/5/3 glycerol, a yeast fermentation lysate of the binary fission and piperine.

The composition of the invention comprises a water-soluble penetration-promoting component and an oil-soluble penetration-promoting component, and changes the penetration mode of the surface layer of the skin through a dual penetration-promoting mode. Wherein the hot spring water is originated from a unique hot spring region group in the world, such as Italy Abanortiel Mei hot spring center, France Allaval hot spring, Japan North sea Dailao hot spring, France Bellinus mountain hot spring basin, France Buller hot spring town, and the like, generally, the water gushes out of the ground from the ground bottom with the depth of hundreds to thousands of meters through the filtration of a plurality of layers of natural mineral rock layers, the gushing temperature ranges from more than twenty degrees to eighty-nine degrees, and the water has rich mineral substances or unique mucilage thereof. The lysate of the yeast fermentation product of the bifidus is a metabolite, a cytoplasmic fragment, a cell wall component and a polysaccharide complex obtained by culturing, inactivating and decomposing bifidobacterium. The lysate of the yeast fermentation product contains active ingredients such as various amino acids, lipids, polysaccharides, adenosine, vitamins, trace minerals, vitamin B group and the like. The active substance obtained by biological technology is easy to be absorbed and utilized by skin, and has effects of softening cutin and promoting absorption of active substance. Piperine is derived from fructus Piperis, is lipophilic substance, and is tetrahydropiperine synthesized from piperine, which is natural alkaloid extracted from Piperaceae plants. It acts on intercellular lipid pathway through epidermal penetration, and can make active ingredient enter into skin by increasing lipid bilayer fluidity and opening intercellular tight junction protein, thereby improving the penetration effect of active ingredient on stratum corneum.

The piperine, the hot spring water and the lysate of the yeast fermentation product in the composition can improve the diffusion speed of the functional components in the skin; the PEG/PPG/polytetramethylene glycol-8/5/3 glycerin and the polyhydric alcohol in the composition can increase the distribution of the functional components to the skin, and the compounding of the components can make the components exert a synergistic effect, thereby avoiding the problems that the permeation promoting effect cannot be balanced and the irritation is easily caused by the use amount of a single permeation promoting path.

Preferably, the permeation-promoting composition containing hot spring water comprises the following components in parts by mass:

the inventor finds that the penetration-promoting composition combined according to the specific proportion not only has good penetration-promoting effect, but also is mild and non-irritant to the skin, and can enhance the skin elasticity.

Preferably, the hot spring water contains the following ingredients in the following concentrations: 0.1-10 mg/L of calcium ions, 0.2-1.0 mg/L of magnesium ions, 0.1-0.5 mg/L of iron ions, 0.01-0.5 mg/L of sodium ions, 0.01-1.0 mg/L of strontium ions, 0.01-0.5 mg/L of fluorine ions, 0.1-10 mg/L of chloride ions and 0.02-1.0 mg/L of lithium ions.

Preferably, the thermal spring water further comprises a vegetal organic matter.

The inventor finds that the hot spring water not only contains various mineral ions to improve the skin quality, but also is rich in plant organic matters, particularly humus from peat or lignite. The humus can change the ion osmotic pressure balance inside and outside the membrane, thereby playing a role in promoting the absorption of active substances.

The invention also provides a preparation method of the permeation-promoting composition containing the thermal spring water, which comprises the following steps: heating piperine, PEG/PPG/polytetramethylene glycol-8/5/3 glycerol and propylene glycol to 35-40 ℃, adding hot spring water and the lysate of the fermentation product of the yeast schizolysis product after the piperine is dissolved, and uniformly stirring to obtain the permeation-promoting composition containing the hot spring water.

The invention also provides the application of the penetration-promoting composition containing the hot spring water in cosmetics.

The invention also provides a cosmetic comprising the following components: the permeation-promoting composition containing hot spring water, tween, squalane, Glycyrrhiza glabra extract and coenzyme Q10.

The invention also provides a preparation method of the cosmetic, which comprises the following steps: premixing tween and squalane, adding into water at the temperature of 80-85 ℃, uniformly stirring, and cooling to the temperature of less than 50 ℃; adding the thermal spring water permeation-promoting composition, and stirring uniformly; adding Glycyrrhrizae radix extract and coenzyme Q10, and stirring.

Compared with the prior art, the invention has the beneficial effects that: the invention provides a permeation-promoting composition with a plurality of permeation-promoting components by adopting natural active substances and combining water-soluble and oil-soluble permeation-promoting raw materials according to a specific proportion. The composition has good permeation promoting effect, good mildness and skin elasticity enhancing effect. When the skin-care product is applied to cosmetics, the percutaneous permeation rate of functional components can be obviously accelerated, no adverse reaction is caused to the skin, and the safety is good.

Detailed Description

To better illustrate the objects, aspects and advantages of the present invention, the present invention will be further described with reference to specific examples.

The various starting materials used in the following examples and comparative examples were selected from conventional commercial products. The hot spring water may be selected from hot spring series products sold by ODYCEA, hot spring water sold by Laboratoire SONIAM, and the like.

Examples 1 to 3

The specific formula of each example of the cosmetic containing the permeation-promoting composition containing the thermal spring water is shown in the table 1, the preparation method of the cosmetic in each example is the same, and the preparation method comprises the following steps:

(1) accurately weighing the components of the raw materials according to the proportion;

(2) heating the phase A to 80-85 ℃.

(3) Premixing the raw materials of the phase B, adding the premixed raw materials into the phase A, homogenizing and stirring the premixed raw materials uniformly, and cooling the mixture to 50 ℃.

(4) Adding the phase C raw materials, and stirring to dissolve uniformly.

(5) Adding the phase D raw materials, and uniformly stirring.

Comparative examples 1 to 6 differ from examples 1 to 3 only in the formulation of the respective products, specifically in the absence of a component of the penetration enhancing composition (phase C), and comparative example 6 in the absence of the penetration enhancing composition (phase C), the specific formulation being shown in table 1.

TABLE 1 formulation tables (parts by weight) of examples 1 to 3 and comparative examples 1 to 6

Test example 1 transdermal permeation Rate

The test method comprises the following steps: fresh pig dorsal skin was debrided of hair and subcutaneous tissue and cut to size for use. Franz horizontal diffusion cell (effective diffusion area 1.5 cm) is adopted²) A skin permeability study was performed. The back skin of the pig was fixed between two diffusion cells, the receiving solution was isotonic PBS (pH 7.0-7.4), the test solutions were the solutions of examples 1-5 and comparative examples 1-5, respectively, and the transdermal test was performed in triplicate in a circulating water bath at 37 ℃. 0.5mL of each sample was taken at 4h, 8h, and 24h after the start of the experiment, and was supplemented with 0.5mL of PBS after sampling. The sample is filtered by a filter membrane of 0.22 mu m to be used as a detection sample, and the cumulative transmittance of the glabridin and the coenzyme Q10 in the sample is respectively detected and calculated.

The detection method comprises the following steps: detecting the content of each active substance by liquid chromatography.

Calculation formula of cumulative transmittance or retention (%) of active material:

the cumulative transmittance (%) — total permeation amount in the receiving solution/total amount in the test solution diffusion cell 100%.

TABLE 2 transdermal permeation Rate results

The results in Table 2 show that the compositions of examples 1 to 3 accelerate the transdermal permeation rate, and the transdermal permeation rate decreases in the absence of a component of the composition in comparative examples 1 to 5, and the transdermal permeation rate is the lowest in comparative example 6.

Test example 2 mildness

The test basis is as follows: according to the cosmetic safety technical specification 2015 edition, a human skin patch test.

The test method comprises the following steps: a 24h closed patch test was used.

30 healthy volunteers were invited, age 20-59 years, without history of allergy.

The selection area is not more than 50mm²And 0.020-0.025 mL of the products of examples 1-3 and comparative examples 1-5 are respectively weighed into a spot tester with a proper spot tester depth of about 1 mm. After cleaning the back of the subject, a patch tester containing the above product was applied to the curved side of the forearm of the subject for 24 hours. The tested person keeps the spot-sticking part dry within 24h, and avoids violent exercise, scratching spot-testing part, long-time sunlight irradiation and the like. And after 24h, removing the tester and marking, and after 30min, judging under sufficient light after the pressure marks disappear. The classification standard of the adverse skin reaction is shown in the following table, and the adverse skin reaction result of each product is shown in the following table 3.

TABLE 3 grading Standard of adverse skin reactions

TABLE 4 sample Patch test results

As can be seen from the results in Table 4, the permeation-promoting composition of the invention has no serious adverse reaction on skin in examples 1-3 and comparative examples 1-6, has no positive reaction in 32 subjects, and has good safety.

Test example 3 Effect on skin elasticity enhancement

The test method comprises the following steps: the skin elasticity before and after the use of the sample was measured by the skin elasticity tester MPA580, and the degree of change in skin elasticity was evaluated from the difference between the values before and after the skin elasticity value R2, where Δ R2 ═ R2 (after use) -R2 (before use), and the greater the Δ R2 value, the more the elasticity was enhanced.

Healthy volunteers were invited to 80 persons, one group of 10 persons each, for a test period of 15 days and 30 days, once a day, in the morning and evening, using the products of examples 1 to 3 and comparative examples 1 to 6, respectively, on the inside of the crank arm, and testing the R2 values before use, 15 days and 30 days after use, respectively. The calculated Δ R2 values are shown in table 5.

TABLE 5 penetration enhancing effect of penetration enhancing compositions on skin elasticity

As can be seen from Table 5, examples 1-3 of the present invention significantly enhanced skin elasticity, while comparative examples 1-5 were much less effective than the examples due to the absence of certain ingredients in the penetration enhancing composition. While comparative example 6 was the least effective.

Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting the protection scope of the present invention, and although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.

Claims

1. A cosmetic comprises a penetration-promoting composition containing hot spring water, Tween, squalane, Glycyrrhiza glabra extract and coenzyme Q10; the permeation-promoting composition containing the thermal spring water comprises the following components in parts by mass:

the hot spring water comprises the following components in concentration: 0.1-10 mg/L of calcium ions, 0.2-1.0 mg/L of magnesium ions, 0.1-0.5 mg/L of iron ions, 0.01-0.5 mg/L of sodium ions, 0.01-1.0 mg/L of strontium ions, 0.01-0.5 mg/L of fluorine ions, 0.1-10 mg/L of chloride ions and 0.02-1.0 mg/L of lithium ions; wherein the hot spring water also comprises plant organic matters, and the plant organic matters are humus; the hot spring water is at least one of ODYCEA hot spring series products and laboratory Soniae Soniam hot spring water.

2. The cosmetic of claim 1, wherein said penetration enhancing composition comprising thermal spring water is prepared by a process comprising the steps of: heating piperine, PEG/PPG/polytetramethylene glycol-8/5/3 glycerol and propylene glycol to 35-40 ℃, adding hot spring water and the lysate of the fermentation product of the yeast schizolysis product after the piperine is dissolved, and uniformly stirring to obtain the permeation-promoting composition containing the hot spring water.

3. The method for preparing the cosmetic according to claim 1, wherein tween and squalane are premixed and added to water of 80-85 ℃, and the mixture is stirred uniformly and cooled to a temperature of less than 50 ℃; adding the thermal spring water permeation-promoting composition, and stirring uniformly; adding Glycyrrhrizae radix extract and coenzyme Q10, and stirring.