KR20130078052A - Antibacterial compositions containing plant extracts or fractions thereof under light intensity - Google Patents

Abstract

PURPOSE: An antibacterial composition which is prepared using plants is provided to ensure excellent antibacterial activity with safety and fewer side effects and to be used in pharmaceutical products, cleaning agents, or cosmetic materials. CONSTITUTION: An antibacterial pharmaceutical composition contains a light-irradiated material of a plant extract or a fraction of the extract, which is prepared from a plant selected from the group consisting of Euphorbia supina, Paulownia coreana Uyeki, Ranunculus sceleratus, Ficus carica L., Pueraria thunbergiana, Apios americana Medikus, Catalpa ovata, Angelica tenuissima, Illicium religiosum, Phytolacca esculenta, Pteridium aquilinum var. latiusculum, Lythrum anceps, Juglans sinensis, Sophora japonica L., Kalopanax pictus, and Orostachys japonica (Maxim.) A. Berger. An antibacterial cleaning agent contains the light-irradiated material as an active ingredient. The light is irradiated at 2,000-18,000 lux for 1-24 hours.

Description

Antimicrobial compositions containing light extracts for plant extracts or fractions thereof

The present invention is selected from the group consisting of celandine beetle, paulownia, wasabi, fig tree, bamboo, Indian potato, old tree, old book, octagonal fennel, periwinkle, fern, buddha flower, fern, walnut tree, painting tree, oak tree and hawthorn It relates to an antimicrobial composition comprising a light irradiation on any one or more plant extracts or fractions thereof.

The antimicrobial agent means a substance which has an antimicrobial action against bacteria, in particular, a substance which has an antimicrobial action by inhibiting a system for synthesizing a cell wall or a protein, or the like, or prepared from such a substance. Antimicrobial agents are widely used to treat diseases caused by bacterial infections, and have been mainly produced by separating or chemically synthesizing them from natural products including mold.

The most representative of the antimicrobials is penicillin, discovered by Alexander Fleming in 1928. Penicillin is the first antimicrobial agent developed by humans. A part of the penicillin beta-lactam is randomly combined with a transpeptidase linking a peptidoglycan molecule, which is a bacterial cell membrane, thereby weakening the cell wall. It does not endure osmotic pressure and bacteria are lysed, which inhibits the growth of bacteria and produces an antibacterial effect. Since the development of penicillin, excellent antimicrobial agents have been developed based on this, and the representative one is methicillin (methicillin) prepared by partially changing the chemical structure of penicillin.

Antimicrobial agents played a role in preventing or treating bacterial infections, but due to the indiscriminate use of antimicrobial agents, there was a problem in which antimicrobial resistant bacteria appeared to be resistant to the existing antimicrobial agents. Antimicrobial-resistant bacteria are bacteria that show resistance to certain antimicrobial agents and do not work. Resistant bacteria transfer resistance to other bacteria so that resistant bacteria continue to increase. Therefore, if resistance occurs, use an antimicrobial with a stronger antimicrobial activity or change to another class of antimicrobial agents. There is a problem.

Among the bacteria that are resistant to antimicrobials, representatives include methicillin resistant Staphylococcus aureus (MRSA), vancomycin resistant enterococci (VRE), vancomycin intermediate Staphylococcus aureus; VISA ) And E. coli P157: H7, which is resistant to at least six antimicrobial agents.

Methicillin-resistant Staphylococcus aureus was first reported in the United Kingdom in 1961 and reported around the world. Methicillin-resistant Staphylococcus aureus is resistant to most antimicrobials as well as methicillin and can be treated with extremely limited antimicrobial agents. There is a problem. In addition, methicillin-resistant Staphylococcus aureus is the most frequent pathogen causing the infection in the hospital, and is serious in that it can be fatal to death when infected with a weak immune system or the elderly.

For example, in 2006, more than 94,000 people were infected with methicillin-resistant Staphylococcus aureus, with more than 19,000 reported dead. In Korea, methicillin-resistant Staphylococcus aureus is an infectious agent of hospitals, and it is reported that it is located almost 80% in large general hospitals.

Increasing resistance to antimicrobials has led to economic and social problems, including increased use of new high-cost antimicrobials, increased treatment duration, and mortality, leading to medical disputes. Therefore, development of new antimicrobial agents is required to solve these problems. However, even if a new antimicrobial agent is developed over time, resistance develops. If a natural product is used, a safe drug with less side effects can be developed while solving this problem. Because natural products contain new ingredients that are not known until now, and because they are a mixture of various components can reduce side effects such as resistance due to the pharmacological action between the complexes.

Euphorbia supina , on the other hand, is a herbaceous herb that belongs to the Great Family , and is native to North America. It grows in a field or field, and its stem splits along the ground with its branches split from the bottom, and its length is 10-25 cm. The leaves are long, elliptical in shape, with reddish brown spots in the center, white milky fluid, and are known to have anti-cancer, detoxification, soothing, hemostatic, and diuretic effects.

In the context of pharmaceutical or cosmetic compositions using cephalobacterial bedbugs, Journal of Pharmacognosy 39 (3); 260-253 (2008) disclose compounds which exhibit antioxidant and antioxidant activity of the Arabidopsis methanol extract and its ethyl acetate fraction.

Paulownia coreana Uyeki is a deciduous tree belonging to the genus Paulownia, a native species of Korea. The paulownia tree is about 15m high, and the bark is light brown with dark brown lines. The bark of paulownia is known as copper skin, and it is known to be effective in bronchial pneumonia, bacterial diarrhea, acute enteritis, acute conjunctivitis, and paulownia leaf is said to be copper leaf and is known to be effective in hot flushes and pustules.

Regarding pharmaceutical or cosmetic compositions using paulownia, Korean Patent Laid-Open Publication No. 10-2009-0021021 discloses a novel compound having antioxidant activity isolated from paulownia inner bark, and Korean Patent Publication No. 10-2008-0028029 No. discloses a cosmetic composition utilizing the antioxidant and anti-inflammatory effects of paulownia leaf extract. In addition, Korean Patent No. 10-0502566 discloses an antimicrobial natural dye including a water extract of paulownia bark.

Wasaccho ( Ranunculus sceleratus ) is a perennial herb that grows on the shores of the Araaceae family, and is also called Kowloon-cho, Swamp Weeper, Frog, Noddongwoo, and Sukryongye. It is known to be effective for arthritis, old ophthalmology, swelling, ulcers and hives.

Regarding pharmaceutical or cosmetic compositions using wasabi, Korean Patent No. 10-0550773 improves blemishes, freckles containing at least one active ingredient in the group consisting of Myocho, Sukryongye, Habaek, reading and Wonhwa extracts. And a cosmetic composition for skin whitening.

Fig tree ( Ficus carica L ) is a deciduous shrub belonging to the family Mulberry family and is native to the western Mediterranean coast of Asia. The bark is 2 ~ 4m high, the bark is gray white, the leaves are alternately thick, broad egg-shaped, the surface is rough and the hair is on the back. Latex is known to be effective for athlete's foot, boil, hemorrhoids, leaves for athlete's foot and neuralgia, fruit for drop in blood pressure, constipation, etc.

Regarding the pharmaceutical or cosmetic composition using the fig tree, Korean Patent No. 10-0999306 discloses an old chicken rehabilitation agent using the fig milk. And the Republic of Korea Patent No. 10-0512285 discloses a medicinal soap composition having a fungal and antibacterial effect containing a fig fruit or leaf extract.

Pueraria thunbergiana is a vine plant belonging to the legume and grows in low mountains and fields all over Korea. The stem extends long and winds up another object, the leaves are shifted, and the edge is flat. 분 squeeze juice, squeeze out, and then squeeze several times with water and dried, called brown powder. It has been known to have diarrhea and thirst quencher, and the juice is known to be effective for headache, thirst and insomnia.

Regarding a pharmaceutical or cosmetic composition using ,, Korean Patent Laid-Open Publication No. 1994-0025579 discloses a composition for preventing hyperlipidemia, suppressing cholesterol, and enhancing liver function, comprising catechin as an active ingredient among polyphenols among 칡 components. . Korean Patent Laid-Open No. 10-2011-0088814 discloses a composition for improving postmenopausal women's health, which has an effect of increasing estrogen concentration, reducing blood triglyceride level, and inhibiting bone mineral density loss, which contain extract as an active ingredient. In addition, the Republic of Korea Patent No. 10-0953800 discloses a composition for preventing white hair and vitiligo containing the roots as an active ingredient.

Indian potato ( Apios americana Medikus ) is a legume native to North America called Apios and American emo, and it is effective in preventing diabetes, obesity, hypertension, atopy, HIV proliferation, osteoporosis, anemia, and nourishment tonic. Known.

Regarding pharmaceutical or cosmetic compositions using Indian potatoes, Japanese Patent Laid-Open No. 2007-332092 discloses a skin whitening and anti-aging cosmetic composition containing an Indian potato extract as an active ingredient.

Catalpa ovata is a deciduous broad-leaved arboreous tree belonging to Jacaranda family, also known as the paulownia, and is distributed in Korea, China, and Japan. Fruits are called fruiting and are known to be effective as a diuretic for nephritis, edema, proteinuria, etc. The inner skin of the tree is called confessional blood and is known to be effective for neuralgia, hepatitis, cholecystitis, jaundice and nephritis.

Regarding the pharmaceutical or cosmetic composition using the roe, Korean Patent No. 10-0985719 discloses a cosmetic composition for preventing and treating atopic dermatitis having an antioxidant effect, including a roe extract and a rose of sharon. And Republic of Korea Patent No. 10-0533768 discloses an antioxidant material isolated from the paulownia fruit, Republic of Korea Patent No. 10-0421625 discloses an anti-inflammatory composition containing the catalposide separated from the cuticle of the paulownia tree as an active ingredient Doing. In addition, Korean Patent Laid-Open Publication No. 10-2010-0001484 discloses a pharmaceutical composition for treating or preventing neurodegenerative diseases caused by dopamine deficiency comprising 4,9-dihydroxy-α-rapacone isolated from an alga as an active ingredient. It is disclosed.

Angelica tenuissima is a perennial herbaceous perennial plant that grows in deep mountain foothills. In oriental medicine, the roots of dried roots in autumn are known to be effective for headache, joint pain, toothache, abdominal pain, diarrhea and eczema.

Regarding the pharmaceutical or cosmetic composition using the original bone, Korean Patent Laid-Open Publication No. 10-2005-0072303 discloses a hair growth inhibitory composition comprising a high bone extract and / or Schizandra chinensis extract as an active ingredient. 2005-0008324 describes a pharmaceutical composition for the prevention and treatment of neurological diseases such as neuronal cell protection and ischemic stroke, which contains Gobon extract. In addition, Korean Patent Laid-Open No. 1999-0053083 discloses 5α-reductase activity inhibition and propionibacterium arc, which can be used for acne, hair loss, seborrheic dermatitis, and the like containing one or more of the group consisting of gobon, rhubarb, betel and flakes. Disclosed is an antimicrobial composition for the essence.

Octalium religiosum refers to the dried fruit of the octagon, an evergreen plant belonging to the family of the eduncles. It is known to be effective in diuretic action and appetite promotion, abdominal bloating and nausea relief, back pain, constipation, cystitis, acute rheumatism and the like, which is mainly used in China.

Regarding the pharmaceutical or cosmetic composition using octagonal fennel, Korean Patent Laid-Open Publication No. 10-2011-0035502 discloses a hand cleaning composition having an antibacterial and bactericidal effect using an extract of cloves or octagonal fennel. -2011-0035501 discloses a fiber deodorant composition having an antibacterial and bactericidal effect using an extract of cloves or octagonal fennel. In addition, Korean Patent No. 10-1041444 discloses an antibacterial soap composition using octagonal fennel, and Korean Patent Publication No. 10-2011-0025715 discloses an antibacterial and antiviral detergent composition to which octagonal distillate tablet concentrate is added. Doing. And the Republic of Korea Patent Publication No. 2003-0079492 discloses a pharmaceutical composition for the prevention and treatment of inflammation-related diseases containing the octagonal fennel extract as an active ingredient, the Republic of Korea Patent Publication No. 2003-0019049 includes the octagonal fennel extract It discloses a composition for preventing and treating diabetes.

Phytolacca esculenta is a perennial herb belonging to the family Liliaceae, native to China. It grows around 1m and leaves are alternate, the ends are narrow and the edges are flat. Roots are known to have the effect of treating nephritis and diuretics.

In connection with a pharmaceutical or cosmetic composition using the pore hole, Japanese Patent Laid-Open No. Hei 1-53612 discloses a hair promoting composition comprising the pore root.

Fern ( Pteridium aquilinum var.latiusculum ) is a perennial plant belonging to the fern, with roots growing more than 1m in the ground, and leaves are shaped like a large triangle. Dried root stems made of powder is effective for parasites and bronchitis is known to be effective.

Regarding pharmaceutical or cosmetic composition using fern, Korean Patent No. 10-0483338 discloses a composition containing a dog fern extract having antibacterial activity and antioxidant effect, and Korean Patent No. 10-0986129 discloses sun rock fern extract It discloses a skin external preparation composition which can be used for the use of anti-aging, whitening, atopic skin improvement, etc. containing the as an active ingredient. Japanese Laid-Open Patent Publication No. 2007-297381 discloses a composition having an inhibitory effect on atherosclerosis containing fern extract.

Lythrum anceps is a perennial herb that is also called perennial herb. It grows up to 1m high in wetlands such as streams and meadows, and blooms in purple in May-August. It is known to be effective in diuresis, branches, cystitis and the like.

Regarding the pharmaceutical or cosmetic composition using the bud flower, Korean Patent No. 10-0852737 discloses a pharmaceutical composition for preventing or treating liver cirrhosis, which contains hairy bud root extract having antioxidant and hepatoprotective effects. Korean Patent Registration No. 0163119 discloses an oral composition having an effect of preventing and symptomatic relief of tooth decay and periodontal disease, which comprises baconthus or brucia extract. In addition, Chinese Patent Publication No. 101637483 discloses a pharmaceutical composition containing flavonoids extracted from Buddha flowers that can be used for chemotherapy.

The walnut tree ( Juglans sinensis ) is a deciduous arboreous tree of the phlegmaceae, about 20m high, and its branches are thick and spread out in all directions. Walnut eggs are known to be effective in eliminating tonic, gangjeong and constipation, and walnut oil is also used to cure skin diseases.

In relation to a pharmaceutical or cosmetic composition using a walnut tree, Korean Patent Laid-Open Publication No. 10-2004-0051784 discloses an antioxidant cosmetic composition containing Gaza, walnut or crude extract, and Korean Patent Publication No. 10-2005-0097578 Discloses a skin moisturizing cosmetic composition comprising a walnut extract. In addition, Korean Patent Laid-Open Publication No. 10-2011-0034150 discloses a cosmetic composition for anti-inflammatory, irritation-releasing and immuno-enhancing skin containing a mixed extract of peach root, plum root, plum root and walnut shell as an active ingredient, Korean Patent Laid-Open Publication No. 10-2009-0018200 discloses a composition for antibacterial, antifungal and antiviral containing walnut extract.

Sophora japonica L. is a deciduous tree of the legume, and the flower buds are called lumps and the fruit is called lumps. Buds are known to be effective for atherosclerosis and hypertension, and fruits, branches, and bark are known to be effective for hemorrhoids.

In relation to a pharmaceutical or cosmetic composition using a painting tree, Korean Patent No. 10-0312735 discloses an anti-inflammatory analgesic composition that can be used for arthritis, edema, pain, etc. containing a lump extract as an active ingredient. -0380865 discloses a pharmaceutical composition for the prevention and treatment of allergic diseases containing lump extract as an active ingredient. In addition, Korean Patent No. 10-0380865 discloses a pharmaceutical composition for preventing or treating osteoporosis containing lump extract, and WO2004 / 002435 discloses a slimming composition containing lump extract. have.

Kalopanax pictus is a deciduous arborescent of the arboraceae, also called Yin -tree, and is distributed in Korea, Japan, Manchuria, and China. Bark is called thawing blood and is known to be effective for neuralgia, back pain, arthritis, tonic, fever, kidney disease, diabetes, and fatigue.

In relation to a pharmaceutical or cosmetic composition using a moth tree, Korean Unexamined Patent Publication No. 2002-0071228 discloses a use of a moth extract which can be used for anti-cancer, anti-microbial, antioxidant, liver function improvement, hangover relief, and the like. 10-2006-0096130 discloses an anti-inflammatory composition containing Haedongpi extract and ginger extract, Republic of Korea Patent No. 10-0588448 discloses a functional food for improving the symptoms of degenerative central nervous system disease containing the extract It is starting. In addition, Korean Patent No. 10-0567456 discloses an AIDS therapeutic agent containing the extract of the oak as an active ingredient, and Korean Patent No. 10-0855314 discloses an antimicrobial pharmaceutical composition and a cosmetic composition comprising the extract of the oak tree and Jindukchal. And it is disclosed with respect to the food composition, Republic of Korea Patent Publication No. 10-1997-0007644 discloses a composition for the protection of liver function containing a syringe isolated from thawed bark of bark of the oak. In addition, Korean Patent Laid-Open Publication No. 10-2008-0101344 discloses an anti-inflammatory analgesic composition comprising tannin-containing Haedongpi extract as an active ingredient, and Korean Patent Laid-Open Publication No. 2003-0033281 discloses carlopanax saponin A isolated from thawed skin. It discloses the use of an anti-cancer agent containing as an active ingredient, Republic of Korea Patent No. 10-0555660 discloses a pharmaceutical composition for treating arthritis as an active ingredient Haedongpi extract.

Orostachys japonica A. Berger is a perennial plant belonging to the family Sedumaceae, with dense leaves, no petioles, and white flowers in September. It grows well on tiled roofs of old temples and is also known as roof keeper. It is known to restore liver function, anticancer and antibacterial effect.

Regarding the pharmaceutical or cosmetic composition using vortex, Korean Patent Laid-Open Publication No. 10-2008-0035047 discloses a composition for preventing or treating gastritis and peptic ulcer including vortic extract, and Korean Patent Laid-Open Publication No. 10-2010-0115567 No. discloses an anti-diabetic complex composition comprising a seungsong extract, Korean Patent No. 10-0966719 discloses a composition for inhibiting obesity and preventing or treating hyperlipidemia comprising a seungsong extract. In addition, the Republic of Korea Patent Publication No. 10-2008-0020030 discloses a cosmetic composition for preventing skin aging containing the vortex extract, the Republic of Korea Patent Publication No. 10-2011-0019262 discloses an antithrombotic composition comprising a vortex extract In addition, Republic of Korea Patent No. 10-0346583 discloses a cosmetic composition for moisturizing the skin containing the Wahsong extract as an active ingredient. In addition, Korean Patent Laid-Open Publication No. 10-1011-0042851 discloses a cosmetic composition that is effective in moisturizing and soothing skin and improving atopic dermatitis using vortex, and Korean Patent No. 10-0614222 includes vortex extract as an active ingredient. Disclosed is a composition for treating colorectal cancer, and Korean Patent No. 10-0525815 discloses an antimicrobial composition comprising an ethanol extract of Wasong, and Korean Patent No. 10-0525816 discloses an antidiabetic agent comprising a polysaccharide extract of Wasong The composition is disclosed.

However, there is no disclosure about the antimicrobial composition irradiated with light to the extract of the plant or a fraction thereof until now.

Accordingly, the present inventors have attempted to develop antimicrobial agents, particularly antimicrobial agents resistant to methicillin, using various plant extracts, and as a result, Arabidopsis, paulownia, wasabi, fig tree, 칡, Indian potato, The ethanol extracts of Roe, Root, Octagonal Fennel, Periwinkle, Fern, Buddha, Fern, Walnut, Painted Tree, Umbrella and Wason effectively kill Staphylococcus aureus, especially methicillin-resistant Staphylococcus aureus, which are the main infectious agents in the hospital. In particular, when the plant extract or its fractions are irradiated with light, the antibacterial effect is enhanced, and the side effects are confirmed to be safe and complete the present invention.

One object of the present invention is a baby bedbug, paulownia, wasabi, fig tree, 칡, Indian potato, Japanese oak, ancient book, octagonal fennel, periwinkle, fern, buddha, fern, walnut tree, painting tree, oak tree and hawthorn It is to provide an antimicrobial composition containing any one or more plant extracts selected from the group consisting of or light fractions thereof irradiated with light as an active ingredient.

Another object of the present invention is to provide an antimicrobial composition wherein the light irradiation exhibits an antimicrobial effect against bacilli, cocci or helix.

Another object of the present invention is to provide the antimicrobial composition for the use of medicines, detergents, cosmetics and the like.

In one embodiment, the present invention is a baby bedbug, paulownia, wasabi, fig tree, 칡, Indian potato, Japanese oak, Gobon, octagonal fennel, perforated, fern, buddha, fern, walnut tree, painting tree, oak tree and wason It provides an antimicrobial composition containing any one or more plant extracts selected from the group consisting of or light fractions irradiated with light as an active ingredient.

In the present invention, the cedars, paulownia, wasabi, fig tree, 칡, Indian potato, old tree, old book, octagonal fennel, periwinkle, fern, buddha flower, fern, walnut tree, painting tree, oak tree and hawthorn See the above for details.

The plant includes leaves, flowers, fruits, seeds, stems, roots, shells and outposts of the plant, and may be used including one or more of these sites.

In the present invention, the plant extract or fractions thereof include not only extracts or fractions by solvents of plants, but also their purified matters, their extracts, fractions or concentrated forms of dried products, and the like.

The solvent for the plant extract is methanol, ethanol, propanol, isopropanol, butanol, pentanol, hexanol, ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, dichloromethane, N, N-dimethylformamide ( DMF), dimethylsulfoxide (DMSO), 1,3-butylene glycol, propylene glycol and water can be used alone or as a mixture of two or more, but are not limited thereto. Preferred are methanol, ethanol, propanol, isopropanol, butanol, pentanol and hexanol.

The solvent for the plant fractions is methanol, ethanol, propanol, isopropanol, normal butanol, isobutanol, pentanol, hexanol, ethylene, acetone, normal hexane, isohexane, cyclohexane, ether, chloroform, ethyl acetate, butyl acetate, Dichloromethane, N, N-dimethylformamide (DMF), dimethylsulfoxide (DMSO), 1,3-butylene glycol, propylene glycol and water may be used alone or as a mixture of two or more, but are not limited thereto. . Preferred are normal hexanol, ethyl acetate, normal butanol and water.

In one specific implementation, the extract of the plant was prepared using ethanol to evaluate the antimicrobial activity of the plant, and then fractionated sequentially using normal hexane, ethyl acetate, normal butanol and water.

In the present invention, the method of preparing the plant extract or fractions thereof is not particularly limited, and for example, cold needle extraction, ultrasonic extraction, reflux extraction and the like may be used.

The purified water is a process for removing suspended solid particles from an extract or a fraction. The purified water may be filtered using a cotton, nylon, or the like, or an ultrafiltration method, a freezing filtration method, a centrifugation method, or the like. In addition, it may further comprise a separation process by various chromatographies (size, charge, hydrophobicity or affinity chromatographic).

The extracts, fractions or purified products can be used as such in liquid form or concentrated and / or dried. The method of concentration and / or drying includes, but is not limited to, freeze drying, vacuum drying, hot air drying, spray drying, vacuum drying, foam drying, high frequency drying, infrared drying and the like.

In the present invention, the light means light emitted from the sun, fluorescent lamps, incandescent lamps, etc., but is not limited thereto. In addition, the light preferably includes both visible light, ultraviolet light and infrared light.

The intensity of illuminance of the light is 2,000 to 18,000 lux, preferably 5,000 to 18,000 lux, and more preferably 10,000 to 18,000 lux. Even when the illuminance of the light is less than 2,000 lux, the light irradiated to the plant extract or fraction of the present invention has an antimicrobial effect, but because the irradiation time is long, it is uneconomical because it is inefficient compared to the energy used, and the amount of light is 18,000 If the lux is exceeded, it is uneconomical compared to the energy used compared to the lower than the illuminance.

On the other hand, the light irradiation time is 1 hour or more, preferably 1 to 24 hours, more preferably 2 to 20 hours. When the irradiation time of the light is less than 1 hour, the antimicrobial effect of the light irradiation on the plant extracts or fractions is not effective compared to the non-irradiation, and when exceeding 24 hours, the antimicrobial effect is increased compared to the irradiation time. It is insignificant and uneconomical for the effect. However, the irradiation time of the light can be adjusted according to the irradiation amount of the light.

In one specific embodiment, the ethanol extract of Arabidopsis vulgaris outpost or each of its fractions was irradiated with light of 0 to 18,000 lux to observe the antimicrobial activity against methicillin-sensitive Staphylococcus aureus and methicillin-resistant Staphylococcus aureus. In the case of non-irradiated light (0 lux), the ethanol extracts and the ethyl acetate fractions of terrestrial bed bugs showed insignificant antimicrobial activity in most of methicillin-sensitive Staphylococcus aureus and methicillin-resistant Staphylococcus aureus. Antimicrobial activity was enhanced. On the other hand, the normal hexane fraction of Arabidopsis ethanol extract showed excellent antimicrobial activity in both methicillin-sensitive Staphylococcus aureus and methicillin-resistant Staphylococcus aureus even when light was not irradiated (0 lux). In the case, the antibacterial activity was further increased. In addition, antimicrobial activity increased as the light intensity of both Arabidopsis ethanol extract and its fractions increased, but the antimicrobial activity tended to decrease at a certain level of illumination. It showed antibacterial activity dependent on the concentration of the fraction.

In another specific embodiment, the ethanol extract of Paulownia leaf or its normal hexane fraction, its ethyl acetate fraction, its normal butanol fraction and its water fraction are irradiated with light of 0 to 18,000 lux, thereby methicillin-sensitive Staphylococcus aureus and methicillin resistance. As a result of observing the antimicrobial activity against Staphylococcus aureus, when the light was not irradiated, methicillin-sensitive Staphylococcus aureus and methicillin-resistant Staphylococcus aureus showed no or insignificant antibacterial activity. The antimicrobial activity was enhanced. In addition, the higher the irradiance of light, the higher the antimicrobial activity, but at a certain level of illuminance, the antimicrobial activity also tended to decrease, and showed the antibacterial activity dependent on the concentration of paulownia leaf extract or fraction.

In another specific embodiment, wasabi stems, fig tree leaves, beech leaves, Indian potato roots, elder branches, botanical roots, octagonal, pericula leaves, fern stems, fern roots, buddha flowers, buddha stems, walnut bark The antimicrobial activity of methicillin-sensitive Staphylococcus aureus and methicillin-resistant Staphylococcus aureus was investigated by irradiating light of 4,000 to 18,000 lux to the ethanol extracts on each of the locust tree branches, the oak leaves, and the hawthorn flowers. The antimicrobial activity was enhanced. In addition, antimicrobial activity increased as the illuminance of the irradiated light increased, but the antimicrobial activity tended to decrease rather than a certain level of illuminance.

Therefore, by adjusting the concentration of the plant extract or fraction, the intensity of light and the irradiation time irradiated to the extract or fraction, it is possible to use the light irradiation for a lesser amount of the plant extract or fraction than the conventional one, but can exhibit the same or more antibacterial activity Because of the economical and minimized side effects can use a safe antimicrobial composition.

In the present invention, the bacterium exhibiting antimicrobial activity to the light irradiated to the plant extract or fraction includes both Gram-positive bacteria and Gram-negative bacteria, but the types of bacillus, cocci, helix, etc. are not limited thereto. to be. The aureus may include diarrhea, staphylococci, streptococci, and the like, more preferably staphylococcus, and staphylococci include Staphylococcus aureus, epidermal staphylococcus, vancomycin-mediated Staphylococcus aureus, and more preferably. Is Staphylococcus aureus, most preferably methicillin resistant Staphylococcus aureus.

In addition, the composition of the present invention may further include an existing antimicrobial agent, the kind is not limited thereto, but penicillin-based antibacterial agent, quinolone (quinolone) -based antimicrobial agent, tetracycline (tetracycline) antibacterial agent, sulfa (sulfonamide) antibacterial agent , Macrolide-based antimicrobial agents, and the like, and preferably penicillin-based antimicrobial agents.

The penicillin-based antimicrobial agent has an antimicrobial effect by affecting bacterial cell wall synthesis and inhibiting bacterial growth. Examples of the penicillin-based antimicrobial agent include penicillin G, penicillin V, methicillin, oxacillin, cloxacillin, and dicloxacillin. (dicloxacillin), nafcillin (nafcillin), ampicillin (ampicillin), amoxicillin, carbenicillin, carbenicillin, tickarcillin, mezlocillin, piperacillin, and piperacillin. It is not necessarily limited to this.

By further including an antimicrobial agent in the antimicrobial composition containing the light irradiation for the plant extract or fractions thereof, the antimicrobial activity can be further enhanced due to the antimicrobial action having different mechanisms, and antimicrobial activity against various kinds of bacteria. Can be represented.

In addition, the content of the existing antimicrobial agent added is preferably 0.001 to 30% by weight based on the total composition, but is not limited thereto. When the content of the antimicrobial agent is less than 0.001% by weight, the antimicrobial effect by the antimicrobial agent may be insignificant, and when the antimicrobial agent exceeds 30% by weight, the antimicrobial agent may be precipitated or may be uneconomical compared to the effect.

The antimicrobial composition of the present invention can be used for various purposes for the purpose of antibacterial, examples thereof include pharmaceuticals, detergents, cosmetics and the like.

In one specific embodiment, the antimicrobial composition is used as an antimicrobial pharmaceutical composition.

The antimicrobial composition included in the pharmaceutical composition of the present invention is 0.0001 to 30% by weight, preferably 0.0001 to 10% by weight based on the total weight of the pharmaceutical composition.

The pharmaceutical composition may be formulated as a conventional agent in the pharmaceutical field according to the therapeutic purpose, for example, tablets, capsules, powders, granules, suspensions, emulsions, syrups, emulsions, warnings, ointments, sprays , Oils, gels, spirits, tinctures, baths, linings, lotions, patches, pads, creams and the like. In addition, it can be preferably used as a topical skin for the purpose of topical application directly applied to the affected area, in this case, the form of an ointment, lotion, spray, gel or the like is preferable. These dermal external preparations may also be included in support bases or matrices that may be applied directly to the treatment site, and examples of the support bases include gauze or bandages. The plant extract or fractions thereof used in the formulation may be in colloidal form or dried powder form or the like.

When formulated as an ointment, skin surface temperature, skin pH, transdermal water loss value, epidermal total lipid value, etc. should be taken into account. Vaseline, liquid paraffin, paraffin, plastibase, silicone, pork (lard), vegetable oils, waxes, formulated in combination with oil-soluble substrates, such as water-soluble substrates, emulsion bases, suspension bases, such as purified lanolin. These ointments include antioxidants (e.g. tocopherol, BHA, BHT, NDGA, etc.), preservatives (e.g., phenolic substances, chlorobutanol, benzyl alcohol, parabens, benzoic acid, etc.), moisturizers (e.g. glycerin, propylene glycol, sorbitol). ), Dissolution aids (e.g., ethanol, propylene glycol), softening aids (e.g., liquid paraffin, glycerin, propylene glycol, surfactants, etc.) and the like.

When formulated into a lotion, it may be prepared in a lotion such as a solution, suspension or emulsion type, and in the case of a lotion applied to the skin, for example, it may be prepared to have a viscosity of 200 cps to 500 cps. It is preferable to prepare a compound by combining a humectant such as glycerin and propylene glycol.

When formulated with a spray, a propellant or the like may be combined with the additives to disperse the dispersed dispersion or wet powder.

When formulated as a patch, absorption accelerators can be used to increase the absorption of the compound through the skin.

The pharmaceutical composition of the present invention can be administered in various dosage forms such as oral, parenteral, topical administration, rectal administration, intranasal administration. For oral administration, for example, oral solids, together with the actives, can be diluted (e.g., lactose, dextrose, sucrose, cellulose, corn starch, potato starch, etc.), lubricants (e.g. silica, talc, stearic acid). , Magnesium or calcium stearate, polyethylene glycol, etc.), binders (e.g. starch, arabic gum, gelatin methylcellulose, carboxymethylcellulose, polyvinyl pyrrolidone, etc.), disintegrants (e.g. starch, alginic acid, alginate, Sodium starch glycolate, etc.), formal mixtures, dyes, sweeteners, wetting agents (eg, lecithin, polysorbates, laurylsulfate, etc.), and other generally pharmacologically inert substances used in pharmaceuticals. When administered topically, excipients (e.g. lactose, starch, cellulose, lactose, polyethylene glycol, etc.), lubricants (e.g. magnesium stearate, stearic acid, glyceryl behenate, talc, etc.), preservatives (e.g. benzyl) Alconium chloride, etc.), and the like.

Parenteral administration includes injections that produce a systemic effect or injections that are administered directly to the suffering area. Examples of parenteral administration include subcutaneous, intravenous, intramuscular, intradermal, intradural, intraocular and general infusion techniques.

Topical administration includes the treatment of areas or organs of infection that are easily accessible by topical application such as, for example, ear, vagina, open and closed wounds or closed wounds and skin, including eye, outer and middle ear infections. It also includes transdermal delivery resulting in systemic effects.

Rectal administration includes the form of suppositories.

Intranasal administration includes nasal aerosol or inhalation applications.

The pharmaceutical composition of the present invention is administered in a pharmaceutically effective amount. In the present invention, a pharmaceutically effective amount means an amount sufficient to treat or prevent a disease at a reasonable benefit / risk ratio applicable to medical treatment or prevention. The effective dose level will depend on the type of disease and its severity, Including the age, weight, health and sex of the patient, sensitivity of the patient to the drug, time of administration of the particular extract used, route of administration and rate of release, duration of treatment, Can be determined according to factors well known in the art. In general, an adult may be administered an amount of 0.1 to 1000 mg / kg per day, preferably a dose of 10 to 100 mg / kg, once to several times daily.

In addition, the present invention relates to a method for treating bacterium, aureus or spiral infection, particularly methicillin resistant Staphylococcus aureus by administering the antimicrobial pharmaceutical composition to a subject.

Such treatment refers to therapeutic treatment and preventive or preventive means. Therefore, those in need of treatment include those infected with methicillin resistant Staphylococcus aureus, as well as those who wish to prevent infection with methicillin resistant Staphylococcus aureus. In addition, the subject refers to all animals that are infected with the fungus and need treatment, specifically, mammals such as humans, cattle, dogs, horses, pigs, guinea pigs, hamsters, squirrels, rodents such as rats, chickens, pheasants, and sparrows. Birds, such as snakes, lizards, turtles, reptiles such as crocodiles, amphibians such as frogs, salamanders, etc., but is not limited thereto.

In another specific embodiment, the antimicrobial composition is used as an antimicrobial detergent composition.

The cleaners refer to materials and products that can build up, remove or disperse solid and liquid dirt from the surface being cleaned, and the cleaning refers to the removal of unwanted materials or microorganisms from the surface.

The antimicrobial composition included in the cleaning agent of the present invention is 0.0001 to 30% by weight, preferably 0.0001 to 20% by weight, most preferably 0.0001 to 10% by weight based on the total weight of the cleaning agent.

Components included in the cleaning agent of the present invention as an active ingredient includes components commonly used in the cleaning agent in addition to the composition for antibacterial. Conventional auxiliaries such as, for example, perfumes, dyes, solvents, opacifiers, copolymer dispersions, thickening agents, cellulose derivatives, pointed gums, albumin derivatives, fats, oils, keratinizers, keratinizers, thickeners, stabilizers, and Carriers and the like.

The detergent of the present invention can be used as a detergent for washing the living environment, such as a detergent for washing the human body such as shampoo, rinse, soap, hand wash, foam cleansing, dishware, bathroom, sink, wallpaper, etc. However, it is not limited thereto.

The detergents of the present invention may be prepared in any formulations conventionally made in the art. Examples are liquids, solids, creams and sprays.

As another specific embodiment, the antimicrobial composition is used as an antimicrobial cosmetic composition.

The antimicrobial composition included in the cosmetic of the present invention is 0.0001 to 20% by weight, preferably 0.0001 to 10% by weight, most preferably 0.0001 to 3.0% by weight based on the total weight of the cosmetic.

Ingredients included in the cosmetic of the present invention as an active ingredient, in addition to the above-described antimicrobial composition includes the components commonly used in cosmetics. Conventional adjuvants such as, for example, thickeners, stabilizers, solubilizers, vitamins, pigments and flavors, and carriers and the like.

Cosmetics of the present invention may be prepared in any formulation conventionally prepared in the art. For example, they may be formulated as solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations and sprays, But is not limited thereto. More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the cosmetic formulation of the present invention is a paste, cream or gel, the carrier component is animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, calcium silicate. Or polyamide powder or the like can be used, and especially in the case of a spray, it can additionally comprise propellants such as chlorofluorohydrocarbons, propane / butane or dimethyl ether.

When the cosmetic formulation of the present invention is a solution or emulsion, a solvent, solubilizing agent or emulsifying agent is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, Fatty acid esters of 1,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the cosmetic formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, micro Crystalline cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the cosmetic formulation of the present invention is a surfactant-containing cleansing agent, the carrier component is aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid. Amide ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

As another aspect, the present invention provides a method for producing an antimicrobial composition comprising the step of preparing any one or more of the plant extracts or fractions thereof and irradiating light to any one or more of the extracts or fractions. do.

In the method for producing the antimicrobial composition of the present invention, the first step is a step of producing a plant extract or a fraction thereof. For the contents of the plant extract or fractions, refer to the above description.

In one specific implementation, plants are prepared using ethanol to extract and then fractionated sequentially using normal hexane, ethyl acetate, normal butanol and water.

The next step is to irradiate the plant extract or fractions thereof with light. When the plant extract or their fractions are irradiated with light, the antimicrobial activity of the plant extracts or their fractions is enhanced, so that high antimicrobial activity can be obtained even using a small amount, which is economical and safe. For the light, the illuminance of the light and the irradiation time of the light, refer to the above description.

In addition, the method may include the step of further mixing the existing antimicrobial agent to the next step. For the antimicrobial agent and its content, refer to the above description.

By further including an antimicrobial agent in the antimicrobial composition containing the plant extract or a fraction thereof, the antimicrobial activity may be further enhanced due to the antimicrobial action having different mechanisms, and may exhibit antimicrobial activity against various types of bacteria.

In addition, the plants are a natural product that has been used for a long time as a folk remedy, so there is almost no toxicity and side effects thereof, and thus can be used without concern for toxicity and side effects in medicines, detergents and cosmetics.

Since the antimicrobial composition of the present invention uses various plants that are natural products, there is little concern of side effects and is safe. The antimicrobial composition is excellent in antimicrobial activity and can be usefully used in medicines, detergents or cosmetics. In particular, since it has excellent antimicrobial activity against Staphylococcus aureus resistant to methicillin, its utility value is very large. In addition, the antimicrobial activity can be enhanced by adjusting the light intensity and irradiation time irradiated to the antimicrobial composition, and the use of a small amount of the antimicrobial composition can exhibit an antimicrobial activity equivalent to or higher than that of a conventional antimicrobial agent. Low risk, economical and efficient In addition, because the plants are easily found in Korea, they can seek their resources and high value-added products.

Hereinafter, the present invention will be described in more detail with reference to examples. These examples are only for illustrating the present invention specifically, it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention.

Example 1: Preparation of Plant Extracts and Fractions

1-1. Collection of plants

Baby Bedbug Outpost, Paulownia Leaf, Wasabi Stem, Fig Tree Leaf, Sesame Leaf, Indian Potato Root, Fir Tree Branch, Root Root, Octagonal Fennel, Lilium Leaf, Fern Stem and Root, Buddha Blossom Stem and Flower, Walnut Fruit Peel, Painting tree branches, oak leaves, and hawthorn flowers were collected in June 2010 in Suncheon. The samples were confirmed by Professor Shin Dong-won of the Department of Herbal Resources, Sunchon National University.

1-2. Plant Extracts and Fractions

The plants collected in Example 1-1 were dried and then boiled in 1 L of ethanol for 3 hours and extracted. Next, the ethanol extract is dissolved in distilled water at a concentration of 10%, and then fractionated in the order of normal hexane (n-hexane), ethyl acetate (EtOAc), normal butanol (n-BuOH) and water having different polarities. Store at 4 ° C.

Example 2 Collection and Culture of Strains

2-1. Collection of strains

Clinical isolates (DPS1-5) for methicillin resistant Staphylococcus aureus (MRSA) were isolated from five patients at Wonkwang University Hospital. The methicillin-resistant strains (methicillin-resistant strain) of S. aureus ATCC 33591 and methicillin-susceptible strains (methicillin-susceptible strain) S. aureus ATCC 25923 was purchased from American Type Culture Collection (ATCC).

2-2. Strain storage and culture method

The bacteria of Example 2-1 were stored in 30% glycerol and then frozen at -70 ° C. The bacteria were cultured using Mueller-Hinton broth (MHB) and Mueller-Hinton agar (MHA), and in Example 2-1 when incubated in Muller-Hinton agar (MHA) The microorganisms were suspended in Muller-Hinton's medium and incubated at 37 ° C. for 24 hours before use.

Example  3: Baby bedbug outpost  Light for Ethanol Extract Investigation  Antimicrobial activity measurement

3-1. Determination of Minimum Inhibitory Concentration

(1) Preparation of Sample

The ethanol extract of Arabidopsis vulgaris outpost was prepared so that the initial concentration was 2000 µg / ml.

(2) determination of minimum inhibitory concentration (MIC);

Minimum inhibitory concentrations were determined by CLSI microdilution broth method. S.aureus ATCC 25923, methicillin susceptible strains of syringe was produced clinically isolated strain of DPS1 to DPS5 for the cylinder of methicillin-resistant strain ATCC 33591 and S.aureus methicillin resistant Staphylococcus aureus at a concentration of 1.5 × 10 ⁸ CFU / ㎖ . Sample (1) of Experimental Example 3-1, prepared at a concentration of 2000 µg / ml, was diluted twice in 50% DMSO sequentially so that the concentration of the sample was from 2000 µg / ml to 0.975 µg / ml. . Then, each well of a 96-well plate was treated with 100 μl of liquid medium, 10 μl of bacterial solution and 10 μl of sample for each concentration, and the plate was not irradiated with light and 2,000, 4,000, 6,000, 8,000, 10,000, 12,000, Incubated for 18 hours at 37 ℃ divided by the conditions of irradiation of light of 14,000, 16,000 and 18,000 lux. Light irradiation conditions were performed by placing the plate in a plant culture chamber (Vision, Korea) equipped with a Shinkwang Electric L-40D compact fluorescent lamp. Minimum inhibitory concentration is determined by the clinical isolated strain to the DPS1 DPS5 growth on cylindrical methicillin-susceptible strains of S.aureus ATCC 25923, a methicillin-resistant strain ATCC 33591 and S.aureus methicillin resistant Staphylococcus aureus completely inhibitory concentration The results obtained through the experiments are shown in Table 1 below.

As a result, the ethanol extract of Arabidopsis vulgaris outpost did not show antimicrobial activity under the light irradiated condition. However, under light irradiation conditions, both methicillin-sensitive Staphylococcus aureus and methicillin-resistant Staphylococcus aureus showed antimicrobial activity, and the antimicrobial activity increased with increasing light intensity. However, when the light intensity is more than a certain degree, the antimicrobial activity was found to decrease. The antimicrobial activity of the light irradiation on the ethanol extracts of A. edodes outpost varies depending on the sample, but was shown to be maximum when irradiated with light of 10,000 to 16,000 lux.

3-2. Determination of Growth Inhibition

(1) Preparation of Strain Suspension

In order to determine the antimicrobial activity of light irradiation against ethanol extracts of Arabidopsis vulgaris outpost by disc diffusion method, methicillin-sensitive strains S.aureus ATCC 25923, methicillin-resistant strains S.aureus ATCC 33591 and methicillin Clinical isolates DPS1 to DPS5 against resistant Staphylococcus aureus were incubated at 37 ° C. for 24 hours in Muller-Hinton agar medium. The strains were then inoculated in Muller-Hinton medium to prepare a suspension with a turbidity of about 1.5 × 10 ⁸ CFU / mL.

(2) preparation of samples

The ethanol extract of Arabidopsis vulgaris outpost was prepared so that the initial concentration was 2000 µg / ml.

(3) inoculation and incubation of samples

After pouring Muller-Hinton agar medium into Petri dishes, the suspension prepared in (1) of Experimental Example 3-2 was added to each Petri dish and plated. A 6 mm diameter sterile paper disk was then placed in the center of the petri dish. Next, 10 μl each of the samples obtained by diluting the ethanol extract of the Arabidopsis vulgaris outpost prepared in (2) of Experimental Example 3-2 on the paper disk of Petri dish sequentially was loaded by 10 μl, respectively, to about 250 μg and 500 Μg. Petri dishes were then incubated in a plant growth chamber at 37 ° C. for 18 hours under conditions of no light and 2,000, 4,000, 6,000, 8,000, 10,000, 12,000, 14,000, 16,000 and 18,000 lux. . Light irradiation conditions were performed by placing the Petri dishes in a plant culture chamber (Vision, Korea) equipped with a Shinkwang Electric L-40D compact fluorescent lamp.

(4) measurement of antimicrobial activity

Antimicrobial activity was shown by measuring the diameter of the strain inhibited growth around each disk after the incubation time of (3) of Example 3-2. The results obtained through the experiment are shown in Table 2 below.

As a result, the ethanol extract of Arabidopsis vulgaris outpost did not show antimicrobial activity when the light was not irradiated except for the DPS2 group, but showed antimicrobial activity when irradiated with light. The antimicrobial activity increased as the light intensity increased, but when the light intensity reached a certain level, the antimicrobial activity decreased. The antimicrobial activity of the light irradiation on the ethanol extracts of Arabidopsis vulgaris outpost varies depending on the sample, but was shown to be maximum when irradiated with light of 10,000 to 16,000 lux.

In addition, all groups tested showed a concentration-dependent antimicrobial activity that was observed when the diameter of the strain inhibited growth was wide when 500ug loading compared to the 250ug loading of the ethanol extract of Arabidopsis vulgaris outpost.

Example 4: Determination of the antimicrobial activity of light irradiated on paulownia leaf ethanol extract

4-1. Determination of Minimum Inhibitory Concentration

(1) Preparation of Sample

The paulownia leaf ethanol extract was prepared so that the initial concentration was 2000 μg / ml.

(2) determination of minimum inhibitory concentration (MIC);

To obtain the minimum inhibitory concentration of the light irradiated to the paulownia ethanol extract, the results obtained by performing the experiment in the method of Example 3-1 are shown in Table 3 below.

The experimental results showed that the ethanol extract of paulownia leaves showed no antimicrobial activity except DPS3, a clinical isolate against methicillin-resistant Staphylococcus aureus, and the antimicrobial activity of DPS3 was very weak. However, under light irradiation conditions, both methicillin-sensitive Staphylococcus aureus and methicillin-resistant Staphylococcus aureus showed antimicrobial activity, and the antimicrobial activity increased with increasing light intensity. However, when the light intensity is above a certain level, the antimicrobial activity was found to decrease. The antimicrobial activity of the light irradiated on the paulownia ethanol extract was different depending on the sample, but it was shown to be maximum when 12,000 to 18,000 lux of light was irradiated.

4-2. Determination of Growth Inhibition

(1) Preparation of Strain Suspension

Methicillin-sensitive strains, S.aureus ATCC 25923, Methicillin-resistant strains, S.aureus ATCC 33591 and Methicillin-resistant yellow, to measure the antimicrobial activity of light irradiated on paulownia ethanol extracts by the disc diffusion method. Clinical isolates DPS1 to DPS5 against Staphylococcus were cultured in Muller-Hinton agar medium for 18 hours at 37 ° C. The strains were then inoculated in Muller-Hinton medium to prepare a suspension with a turbidity of about 1.5 × 10 ⁸ CFU / mL.

(2) preparation of samples

The paulownia leaf ethanol extract was prepared so that the initial concentration was 2000 μg / ml.

(3) inoculation, incubation of samples and determination of antimicrobial activity

In order to measure the antimicrobial activity of the light irradiated against the paulownia ethanol extract, the results obtained by performing the experiment by the method of Example 3-2 are shown in Table 4 below.

As a result of the experiment, paulownia leaf ethanol extract did not show antimicrobial activity in all groups when it was not irradiated with light, but it was confirmed that antimicrobial activity appeared when irradiated with light. The antimicrobial activity increased as the light intensity increased, but when the light intensity reached a certain level, the antimicrobial activity decreased. The antimicrobial activity of the light irradiation on the ethanol extract of paulownia leaves was shown to be maximum when irradiated with light of 12,000 to 18,000 lux, depending on the sample.

In addition, all groups tested showed a concentration-dependent antimicrobial activity that was broadly observed in which the growth of the strain was inhibited in the case of 500 ㎍ loading of ethanol extract of paulownia leaves.

Example 5 Determination of the Antimicrobial Activity of Light Irradiation on Aloe Versus Outpost Fractions

5-1. Determination of Minimum Inhibitory Concentration

(1) Preparation of Sample

Samples were prepared with normal hexane (n-hexane) fraction and ethyl acetate (EtOAc) fraction of Arabidopsis ethanol extract ethanol extract to an initial concentration of 2000 μg / ml.

(2) determination of minimum inhibitory concentration (MIC);

In order to obtain the minimum inhibitory concentration of the light irradiated to the normal hexane (n-hexane) fraction and ethyl acetate (EtOAc) fraction of Arabidopsis ethanol extract ethanol extract, the results obtained by performing the experiment by the method of Example 3-1 Table 5 shows.

Experimental results showed that the ethyl acetate (EtOAc) fraction of Arabidopsis vulgaris outpost was methicillin-sensitive Staphylococcus aureus and methicillin-resistant Staphylococcus aureus under light-free conditions. Appeared. However, under light irradiation conditions, antimicrobial activity was enhanced in both methicillin-sensitive Staphylococcus aureus and methicillin-resistant Staphylococcus aureus, and the antimicrobial activity increased with increasing light intensity. However, when the light intensity is above a certain level, the antimicrobial activity was found to decrease.

However, the normal n-hexane fraction of A. elata was excellent in both methicillin-sensitive Staphylococcus aureus and methicillin-resistant Staphylococcus aureus under light-irradiated conditions. The antimicrobial activity increased further. In addition, the antimicrobial activity increased as the light intensity increased, but when the light intensity was above a certain level, the antimicrobial activity was found to decrease.

The antimicrobial activity of the light irradiation on the normal hexane (n-hexane) fraction and ethyl acetate (EtOAc) fraction of the Arabidopsis vulgaris outpost varied depending on the sample, but was shown to be maximum when irradiated with light of 10,000 to 18,000 lux.

5-2. Determination of Growth Inhibition

(1) Preparation of Strain Suspension

S. aureus , a methicillin susceptible strain, was used to determine the antimicrobial activity of light irradiated on A. elata fraction by the disc diffusion method. ATCC 25923, the methicillin resistant strain S. aureus ATCC 33591 and the clinical isolates DPS1 to DPS5 against methicillin resistant Staphylococcus aureus were cultured in Muller-Hinton agar medium for 24 hours at 37 ℃. The strains were then inoculated in Muller-Hinton medium to prepare a suspension with a turbidity of about 1.5 × 10 ⁸ CFU / mL.

(2) preparation of samples

Samples were prepared to test normal hexane (n-hexane) fraction and ethyl acetate (EtOAc) fraction of Arabidopsis ethanol ethanol extract to an initial concentration of 2000 μg / ml.

(3) inoculation, incubation of samples and determination of antimicrobial activity

In order to measure the antimicrobial activity of the light irradiation to the normal hexane (n-hexane) fraction and ethyl acetate (EtOAc) fraction of Arabidopsis ethanol extract ethanol extract, the results obtained by performing the experiment by the method of Example 3-2 are as follows. Table 6 shows.

As a result, the normal hexane (n-hexane) fraction of Arabidopsis vulgaris outpost showed antimicrobial activity in both methicillin-sensitive Staphylococcus aureus and methicillin-resistant Staphylococcus aureus under the light irradiation, and ethyl acetate (EtOAc). The fractions differed between groups but showed antimicrobial activity against methicillin-resistant Staphylococcus aureus under conditions that were not irradiated with light. In addition, the antimicrobial activity was increased as the light intensity increased, but when the light intensity became more than a certain degree, the antimicrobial activity was found to decrease.

The antimicrobial activity of the light irradiation on the normal hexane (n-hexane) fraction and ethyl acetate (EtOAc) fraction of the Arabidopsis vulgaris outpost varied depending on the sample, but was shown to be maximum when irradiated with light of 10,000 to 18,000 lux.

In addition, all groups tested showed a concentration-dependent antimicrobial activity that was observed when the diameter of the strain inhibited the growth of 500 μg when compared to the 250 μg loading of the Arabidopsis vulgaris outpost.

Example 6: Determination of the antimicrobial activity of light irradiated on paulownia leaf fraction

6-1. Determination of Minimum Inhibitory Concentration

(1) Preparation of Sample

Normal concentrations of n-hexane fraction, ethyl acetate (EtOAc) fraction, normal butanol (n-BuOH) fraction, water fraction, ampicillin, and oxacillin of paulownia ethanol extracts at the initial concentration of 2000 Samples were prepared to be μg / ml. Ampicillin and oxacillin were used as positive controls.

(2) determination of minimum inhibitory concentration (MIC);

Minimum hexane (n-hexane) fraction, ethyl acetate (EtOAc) fraction, normal butanol (n-BuOH) fraction, water fraction, paulownia leaf ethanol extract, light irradiation for ampicillin and oxacillin The results obtained by performing the experiment by the method of Example 3-1 to obtain the inhibitory concentration are shown in Table 7 below.

As a result, the normal hexane (n-hexane) fraction, ethyl acetate (EtOAc) fraction, normal butanol (n-BuOH) fraction and oxacillin of paulownia leaves were methicillin susceptible Staphylococcus aureus and All of the methicillin-resistant Staphylococcus aureus samples differed from sample to sample, but showed generally poor antimicrobial activity. Ampicillin showed mild antimicrobial activity in methicillin-resistant Staphylococcus aureus under conditions that were not irradiated with light, but showed excellent antimicrobial activity in Methicillin-sensitive Staphylococcus aureus. However, under light irradiation conditions, antimicrobial activity was enhanced in both methicillin-sensitive Staphylococcus aureus and methicillin-resistant Staphylococcus aureus, and the antimicrobial activity increased with increasing light intensity. However, when the light intensity is above a certain level, the antimicrobial activity was found to decrease.

The antimicrobial activity of light irradiated on normal hexane (n-hexane) fraction, ethyl acetate (EtOAc) fraction, normal butanol (n-BuOH) fraction, and water fraction of paulownia leaves differs from sample to sample, but light of 12,000 to 18,000 lux Investigations have been shown to be maximum.

6-2. Determination of Growth Inhibition

(1) Preparation of Strain Suspension

S. aureus , a methicillin-sensitive strain, was used to determine the antimicrobial activity of light irradiation on paulownia leaf fractions by the disc diffusion method. ATCC 25923, S.aureus ATCC 33591, a methicillin resistant strain, and DPS1 and DPS2, clinical isolates against methicillin resistant Staphylococcus aureus, were incubated in Muller-Hinton agar for 24 hours at 37 ° C. The strains were then inoculated in Muller-Hinton medium to prepare a suspension with a turbidity of about 1.5 × 10 ⁸ CFU / mL.

(2) preparation of samples

Normal concentrations of n-hexane fraction, ethyl acetate (EtOAc) fraction, normal butanol (n-BuOH) fraction, water fraction, ampicillin and oxacillin of paulownia ethanol extracts were 2000 µg. Samples to be tested were prepared to be / ml, and ampicillin and oxacillin were used as positive controls.

(3) inoculation, incubation of samples and determination of antimicrobial activity

Antimicrobial Activity of Light Irradiation on Normal Hexane (N-hexane) Fraction, EthOAc Fraction, Normal Butanol (n-BuOH) Fraction, Water Fraction, Ampicillin and Oxacillin from Paulownia ethanol Extract The results obtained by performing the experiment by the method of Example 3-2 to measure the activity are shown in Table 8 below.

Experimental results show that the normal hexane (n-hexane) fraction, ethyl acetate (EtOAc) fraction, normal butanol (n-BuOH) fraction and water fraction of paulownia leaves are methicillin susceptible Staphylococcus aureus and methi without light. All of the strains resistant to Staphylococcus aureus showed no antimicrobial activity. However, when irradiated with light, antimicrobial activity was observed, and as light intensity increased, antimicrobial activity also increased. However, when the light intensity is above a certain level, the antimicrobial activity was found to decrease.

The antimicrobial activity of the light irradiated on the normal hexane fraction, ethyl acetate (EtOAc) fraction, normal butanol fraction (n-BuOH) and water fraction of the paulownia leaves varies depending on the sample, but light of 10,000 to 16,000 lux When it is surveyed, it becomes maximum.

In addition, all groups tested had a concentration-dependent antimicrobial activity that was observed to be wider when the growth of the strain was inhibited when 500 μg was loaded compared to 250 μg loading of the paulownia leaf.

Ampicillin, a positive control group, showed antimicrobial activity in all groups, irrespective of whether or not it was irradiated with light.Increasing light intensity showed an increase in antimicrobial activity, but the difference was not large. When the more than a certain degree, the antimicrobial activity was found to decrease. However, oxacillin did not show antimicrobial activity except for the DPS2 group.

Example 7: Determination of the antimicrobial activity of light irradiation on other plant extracts

7-1. Preparation of samples

Wasabi stem, Fig leaf, Beet leaf, Indian Potato root, Alder branch, Root root, Octagonal Fennel, Periwinkle leaf, Fern stem, Fern root, Buddha flower, Buddha stem, Walnut fruit bark, Painting tree branch, Oak leaf and The ethanol extract of each of the pine flowers was prepared so that the initial concentration was 2000 µg / ml.

7-2. Determination of Minimum Inhibitory Concentration

Wasabi stem, Fig leaf, Beet leaf, Indian Potato root, Alder branch, Root root, Octagonal Fennel, Periwinkle leaf, Fern stem, Fern root, Buddha flower, Buddha stem, Walnut fruit bark, Painting tree branch, Oak leaf and In order to obtain the minimum inhibitory concentration of the light irradiation for each ethanol extract of the pine flowers, the results obtained by performing the experiment by the method of Example 3-1 are shown in Table 9 below.

Experimental results show that wasabi stems, ficus leaves, beech leaves, Indian potato roots, elder branches, botanical roots, octagonal fennel, pericardial leaves, fern stems, fern roots, buddha flowers, buddha stems, and walnuts under unlighted conditions No antimicrobial activity of methicillin-sensitive Staphylococcus aureus and methicillin-resistant Staphylococcus aureus was observed in the ethanol extracts of the fruit bark, the Prunus branches, the oak leaves, and the hawthorn flowers. However, under light irradiation conditions, both methicillin-sensitive Staphylococcus aureus and methicillin-resistant Staphylococcus aureus showed antimicrobial activity, and the antimicrobial activity increased with increasing light intensity. However, when the light intensity is above a certain level, the antimicrobial activity was found to decrease. The antibacterial activity of the light irradiated with respect to the ethanol extract of the plants was shown to be the maximum when the light of 12,000 to 18,000 lux, depending on the type of plant and the type of bacteria.

Example 8: Acute Oral Toxicity Test

In order to confirm that the plant extract of the present invention is non-toxic, an acute oral toxicity test was conducted. Twenty five to six week old SPF SD rats were used as experimental animals to perform acute toxicity testing under the following conditions.

Temperature, humidity conditions: temperature 22 degrees +/- 2 degrees, relative humidity 50 +/- 10%

Contrast cycle: Fluorescent light illumination (lights off at 8:00 after lighting at 8:00)

Roughness: 200 to 300 lux

Free treatment of UV-treated drinking water

A test sample was prepared by diluting the test substance in sterile distilled water and adding the same amount using sterile distilled water as a control.

On the day of administration, the general condition is observed every hour up to 4 hours, and once a day from the day after the administration, the general condition changes, poisoning symptoms, mobility, appearance, autonomic nerves, and the presence of dead animals are carefully observed. Pathological anatomical verification was performed to determine the toxicity. As a result, LD ₅₀ was 3000 mg / kg BW and no toxicity was observed.

Claims (11)

At least one selected from the group consisting of celandine beetle, paulownia, wasabi, fig tree, 칡, Indian potato, oak, ancient book, octagonal fennel, periwinkle, fern, buddha flower, fern, walnut tree, painting tree, oak tree, and hawthorn An antimicrobial pharmaceutical composition containing a light irradiated with plant extracts or fractions thereof as an active ingredient.
At least one selected from the group consisting of celandine beetle, paulownia, wasabi, fig tree, 칡, Indian potato, oak, ancient book, octagonal fennel, periwinkle, fern, buddha flower, fern, walnut tree, painting tree, oak tree, and hawthorn Antimicrobial detergent composition containing a light irradiated with light to the plant extract or a fraction thereof as an active ingredient.
At least one selected from the group consisting of celandine beetle, paulownia, wasabi, fig tree, 칡, Indian potato, oak, ancient book, octagonal fennel, periwinkle, fern, buddha flower, fern, walnut tree, painting tree, oak tree, and hawthorn Antimicrobial cosmetic composition containing a light irradiated with light to plant extracts or fractions thereof as an active ingredient.
The composition as claimed in claim 1, wherein the light has an illuminance of 2000 lux to 18,000 lux.
The composition according to claim 1, wherein the irradiation time of light is 1 to 24 hours.
According to claim 1, wherein the bacterium is a composition characterized in that at least one bacteria selected from the group consisting of bacilli, cocci and helix.
The composition according to claim 6, wherein the cocci are at least one cocci selected from the group consisting of diplococci, staphylococci and streptococci.
8. The composition according to claim 7, wherein the staphylococcus is at least one staphylococcus selected from the group consisting of Staphylococcus aureus, Epidermal Staphylococcus and Vancomycin-mediated Staphylococcus aureus.
The composition of claim 8, wherein the Staphylococcus aureus is methicillin resistant Staphylococcus aureus.
The composition of claim 1, wherein the extract is extracted with one or more solvents selected from the group consisting of methanol, ethanol, propanol, isopropanol, butanol, pentanol and hexanol.
The composition of claim 1, wherein the fraction is fractionated with at least one solvent selected from the group consisting of normal hexane, ethyl acetate, normal butanol and water.