KR101392810B1 - Antibacterial compositions containing plant extracts or fractions - Google Patents

Abstract

The present invention relates to an antimicrobial composition comprising, as an active ingredient, at least one plant extract or a fraction thereof selected from the group consisting of mulberry, yin, mulberry, persimmon, persimmon, miso paste and branch. The composition for antimicrobial use produced by using the plant of the present invention exhibits an antimicrobial activity against methicillin-resistant Staphylococcus aureus as well as methicillin-susceptible Staphylococcus aureus. Especially, when a conventional antimicrobial agent is further contained, the composition exhibits a synergistic effect, The value is very large. That is, even if a small amount of the antimicrobial agent is used, the antimicrobial activity of the antimicrobial agent can be equal to or higher than that of the conventional antimicrobial agent, so that the risk of side effects caused by the abuse of the antimicrobial agent is low, economical and efficient. In addition, since plants that are easily found in Korea are used, they can be recycled and high value-added products can be sought.

Description

[0001] The present invention relates to an antimicrobial composition containing a soybean paste extract or fractions thereof,

The present invention relates to an antimicrobial composition comprising, as an active ingredient, at least one plant extract or a fraction thereof selected from the group consisting of mulberry, yin, mulberry, persimmon, persimmon, miso paste and branch.

The term "antimicrobial agent" refers to a substance that has an antibacterial action against bacteria, specifically, a substance that has antimicrobial activity by inhibiting a system in which a bacterium synthesizes cell walls or proteins or the like. Antimicrobial agents are widely used for the treatment of diseases caused by bacterial infections and the like, and they have been produced by separation from natural materials including fungi or by chemical synthesis.

The most common antimicrobial agent is penicillin discovered by Allen Sander Fleming in 1928. Penicillin is the first antimicrobial agent developed by mankind. Part of penicillin beta-lactam binds to transpeptidase, which connects peptidoglycan molecules, which are bacterial cell membranes, to prevent bacterial cell walls from growing. It does not withstand the osmotic pressure, it causes the bacteria to lyse, inhibiting the growth of the bacteria and giving the antibacterial effect. Since the development of penicillin, excellent antibacterial agents have been developed on the basis of this, and a representative example is methicillin which is made by partially changing the chemical structure of penicillin.

Antimicrobial agents played many roles in preventing or treating bacterial infections, but they were resistant to conventional antimicrobial agents due to indiscriminate use of antimicrobial agents, resulting in the emergence of antimicrobial resistant bacteria that are not effective. Antimicrobial-resistant bacteria refers to bacteria that do not respond to specific antimicrobial agents because they are tolerant to certain antimicrobial agents. Resistant bacteria transfer resistance to other bacteria, which causes the resistant bacteria to continue to grow. Therefore, if resistance occurs, use antimicrobial agents with stronger antimicrobial activity or other antimicrobial agents .

Among the various bacteria resistant to antimicrobial agents, methicillin resistant Staphylococcus aureus (MRSA), vancomycin resistant enterococci (VRE), vancomycin intermediate Staphylococcus aureus (VISA) ), And E. coli P157: H7 resistant to at least six antimicrobial agents.

Methicillin-resistant Staphylococcus aureus was first reported in the UK in 1961 and has been reported in various parts of the world. Methicillin-resistant Staphylococcus aureus is resistant to most antimicrobial agents as well as methicillin, There is a problem. In addition, methicillin-resistant Staphylococcus aureus is the most frequent causative agent of pathogens in hospitals, and it is serious in that infected persons or elderly people may be fatal to death.

In 2006, for example, more than 94,000 people in the US were infected with methicillin-resistant Staphylococcus aureus, of which more than 19,000 were reported to have died. In Korea, methicillin-resistant Staphylococcus aureus is an infection pathogen of the hospital, and it is reported that it is over 80% in large general hospitals.

Increased tolerance to antimicrobial agents increases economic and social problems, such as increased use of new high-priced antimicrobial agents, increased duration of treatment, increased mortality, and causing medical disputes. Therefore, development of new antimicrobial agents is required to solve these problems. However, development of a new antimicrobial agent is time-consuming and tolerant. When natural products are used, safe drugs with less side effects can be developed. This is because natural products contain new components that are not known to date, and because they are a mixture of various components, the side effects of tolerance and the like can be reduced due to the pharmacological action between the complexes.

On the other hand, Kalimeris yomena Kitam ) is a perennial plant belonging to the family Asteraceae. It is distributed in Korea, Japan, and China. It is about 30 ~ 100cm in height. Leaves are alternate phyllotaxis with thick sawtooth on the edge. Flowers bloom from July to October. It is known to be effective for tonsillitis, bronchitis, shinhae, geomorphic, cough, asthma, insect bites and so on.

The Korean Society of Food Science and Nutrition, 34 (1), 8-12 (2005), discloses the anticancer effect of fractions on the methanol extract of Mugwort mussel in relation to pharmaceutical or cosmetic composition using mugwort.

Agrimonia pilosa L. is a perennial herb that belongs to the Rosaceae family. Hemostats, diarrhea, enteritis, kidney stones, cholelithiasis and is known to be effective.

Korean Patent Registration No. 10-0327762 discloses a strawberry herb extract having the ability to inhibit hepatitis B virus surface antigen production and Korean Patent Laid-Open No. 10-2007-0089434 discloses Korean Patent No. 10-0809797 discloses a composition for controlling anthrax of a plant using a root extract of strawberry roots, and a composition for preventing or treating anthrax, . Korean Patent Laid-Open Publication No. 10-2010-0120766 discloses a cosmetic composition for improving skin wrinkles containing at least one of yulgok, blood-drawing, and horseradish extract, Korean Patent Publication No. 10-2010-0123456 discloses a cosmetic composition for improving skin wrinkles, Which comprises at least one extract selected from the group consisting of a plant extract, a tree extract, a spine herb extract, and a strawberry herb extract, for preventing and treating acne and improving seborrheic dermatitis. Korean Patent Laid-open Publication No. 10-2011-0075336 discloses an analgesic composition comprising a strawberry herb extract, and Korean Patent No. 10-0763035 discloses a cosmetic composition for acne improvement containing an extract of Seonghak extract. Journal of the Korean Society of Food Science and Nutrition 34 (2), 244 ~ 248 (2004), discloses the antibacterial effect of the extract of yuliaeta on foodborne pathogens.

Rhein is a plant extract having the chemical formula of C ₁₅ H ₈ O ₆ (4,5-Dihydroxyanthraquinone-2-carboxylic acid). It can be extracted from rhubarb, It is known to have efficacy.

Regarding pharmaceutical or cosmetic compositions using lanes, Korean Patent Laid-Open No. 10-2005-0092820 discloses a composition for controlling plant pathogens, which comprises as an active ingredient at least one compound selected from the group consisting of emodine, lane and derivatives thereof .

Gasshorn ( Nardostachys chinensis Batalin ) is a perennial herb that belongs to the matariaceae and grows in the alpine pastures and meadows. It is known to have efficacy in dandruff, vomiting, anorexia, toothache, tooth decay, hemorrhoids, spots and so on.

 Regarding pharmaceutical or cosmetic composition using ginseng, Korean Patent Publication No. 2000-0020728 discloses a skin lightening composition containing as an active ingredient an extract of at least one selected from the group consisting of ginseng extract, hull extract and amber extract Korean Pat. No. 10-1023780 discloses a pharmaceutical composition for preventing and treating acute pancreatitis comprising an extract of Gamma ray extract as an active ingredient, Korean Patent Publication No. 10-2011-0098500 discloses a pharmaceutical composition for preventing and treating acute pancreatitis, As an active ingredient, a composition for the prevention and treatment of type 1 diabetes.

Hydnocarpi semen) are known to be effective standing daepungja seed of a tree or plant dongsok relatives leprosy, itching and so on.

Korean Patent No. 10-0975426 discloses a pharmaceutical composition for the prevention and treatment of allergic dermatitis or atopic dermatitis containing the extract of Ganoderma lucidum extract, Discloses a composition for prevention and treatment of diabetic ulcer comprising extract of Artemisia sp. And Korean Patent No. 10-0966155 discloses a composition for prevention and treatment of ischemic diseases containing Artemisia sp. Extract.

Desmodium caudatum is a deciduous broad-leaved stem of soybean with a height of about 1.5m. It is also called as a hairpin and a hairpin grass. Leaves are known to be effective for gastroenteritis, dysentery, and joint pain.

Regarding the pharmaceutical or cosmetic composition using the soybean paste, Korean Patent Registration No. 10-0543744 discloses a fermented liquid for controlling and treating pine tree disease including oak, fruit, oak, and miso grass.

The branch was named Kochia , an annual herb, scoparia L. ), which is also known as asthma, rhabdomyolysis, chest pain, pain, diuretic, cystitis, constipation and various inflammation.

Regarding pharmaceutical or cosmetic composition using a pesticide, Korean Patent Laid-Open Publication No. 2002-0004192 discloses a composition for treating and preventing obesity containing dietary fiber, chitosan, garnichia cambogia extract and herbal extracts in a leaf extract or an elderberry extract Korean Patent Laid-Open Publication No. 2002-0004193 discloses a composition for removing hangover and protecting soy sauce, which comprises extracts of Chrysanthemum japonica extract or Araliacea japonica extract with milk seed extract, and Korean Patent No. 10-0867509 Discloses a mask pack for preventing and alleviating skin diseases containing a leaf extract. Korean Patent Registration No. 10-0981407 discloses a cosmetic composition containing a natural antimicrobial complex composed of a lotus leaf, a leaflet, and a leaf extract, and Korean Patent No. 10-1039532 discloses a cosmetic composition comprising a lotus leaf, Discloses a cosmetic composition for improving acne containing a natural antimicrobial complex.

However, there has been no disclosure about a composition for antimicrobial use containing an extract of the plant or a fraction thereof as an active ingredient, particularly, an antimicrobial composition exhibiting a synergistic effect when a conventional antimicrobial agent is further contained in the composition.

Accordingly, the present inventors have attempted to develop an antimicrobial composition exhibiting synergistic effect when a composition for antimicrobial use, especially a conventional antimicrobial agent, is further contained using various plant extracts. As a result, it has been found that an antimicrobial composition containing, as an active ingredient, any one or more ethanol extracts or fractions thereof selected from the group consisting of mulberry leaves, yuliai, lane, ginseng root, In particular, the present invention has been accomplished by confirming that methicillin-resistant Staphylococcus aureus is effectively killed as well as methicillin-susceptible Staphylococcus aureus, which is a major staphylococcus in a hospital, and that side effects are small and safe.

It is an object of the present invention to provide an antimicrobial composition containing as an active ingredient at least one plant extract or a fraction thereof selected from the group consisting of mulberry leafhopper, yuliaeta, lane, ginseng root, .

Another object of the present invention is to provide an antimicrobial composition exhibiting a synergistic effect when the composition further comprises a conventional antimicrobial agent such as a penicillin series, a quinolone series, a tetracycline series, a cephalosporin series or a macrolide series .

It is another object of the present invention to provide an antimicrobial composition in which the composition exhibits an antimicrobial effect against bacillus, staphylococci or staphylococci.

It is still another object of the present invention to provide the composition as a medicine, a detergent, a cosmetic or the like.

In one aspect, the present invention provides an antimicrobial composition comprising, as an active ingredient, at least one plant extract or a fraction thereof selected from the group consisting of a mugwort, a lynx, a lane, a ginseng root, .

In the present invention, the above-mentioned contents of the above-mentioned mites, yongjiao, lane, ginseng, daejangja, miso paste and branch are referred to above.

Such plants include leaves, flowers, fruits, seeds, stems, roots, husks and outposts of plants, and these plants may be used in combination with one or more of these plants.

In the present invention, the plant extract or the fraction thereof includes not only an extract or a fraction obtained by a solvent of a plant, but also enriched or dried forms of the purified water, the extract, the fraction or the purified water thereof.

The solvent for the plant extract is selected from the group consisting of methanol, ethanol, propanol, isopropanol, butanol, pentanol, hexanol, ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, dichloromethane, N, DMF), dimethylsulfoxide (DMSO), 1,3-butylene glycol, propylene glycol, and water may be used alone or as a mixture of two or more thereof, but are not limited thereto. Preferred are methanol, ethanol, propanol, isopropanol, butanol, pentanol and hexanol.

The solvent for the plant fractions may be selected from the group consisting of methanol, ethanol, propanol, isopropanol, n-butanol, isobutanol, pentanol, hexanol, ethylene, acetone, normal hexane, isohexane, cyclohexane, ether, chloroform, ethyl acetate, But are not limited to, dichloromethane, N, N-dimethylformamide (DMF), dimethylsulfoxide (DMSO), 1,3-butylene glycol, propylene glycol and water alone or as a mixture of two or more thereof . Preferably normal hexanol, ethyl acetate, normal butanol and water.

In one specific implementation, the plant extracts were prepared using ethanol to assess the antimicrobial activity of the plants, and then sequentially fractionated using normal hexane, ethyl acetate, normal butanol and water.

In the present invention, the method for producing the plant extract or its fractions is not particularly limited, and for example, cold extraction, ultrasonic extraction, reflux cooling extraction and the like can be used.

The purified water is a process for removing suspended solid particles from an extract or a fraction. The purified water may be filtered using a cotton, nylon, or the like, or an ultrafiltration method, a freezing filtration method, a centrifugation method, or the like. In addition, it may further comprise a separation process by various chromatographies (size, charge, hydrophobicity or affinity chromatographic).

The extract, fraction or purified product may be used as it is in liquid form or may be used by concentrating and / or drying it. The method of concentration and / or drying includes, but is not limited to, freeze drying, vacuum drying, hot air drying, spray drying, vacuum drying, foam drying, high frequency drying, infrared drying and the like.

In the present invention, the bacterium includes both Gram-positive bacteria and Gram-negative bacteria, and is not limited to rod-shaped bacterium, spherical bacteria, spiral-like serpentine, and the like. The staphylococci include staphylococci paired with staphylococci, staphylococci streptococci clusters like staphylococcus, strep streptococci in which staphylococci are arranged in rows or chains, and more preferably staphylococci among the staphylococci. Examples of the staphylococci include Staphylococcus aureus, Staphylococcus epidermidis, Vancomycin-mediated Staphylococcus aureus, and preferably Staphylococcus aureus. The Staphylococcus aureus includes methicillin-sensitive Staphylococcus aureus and methicillin-resistant Staphylococcus aureus. More preferably methicillin-resistant Staphylococcus aureus.

In another aspect, the present invention provides an antimicrobial composition characterized by further comprising a conventional antimicrobial agent in the composition.

The antimicrobial agent refers to a conventional antimicrobial agent that is commercially available and is not limited thereto. Examples of the antimicrobial agent include penicillin series antibiotics, quinolone series antibacterial agents, tetracycline antibacterial agents, cephalosporin antibacterial agents, , Macrolide series antibacterial agents and the like, preferably penicillin series antibacterial agents, more preferably ampicillin or oxacillin.

The penicillin-based antimicrobial agent chemically has a beta-lactam ring and inhibits bacterial growth by affecting bacterial cell wall synthesis. Examples of the antimicrobial agent include penicillin G, penicillin V, But are not limited to, methicillin, oxacillin, cloxacillin, dicloxacillin, nafcillin, ampicillin, amoxicillin, carbenicillin, But are not necessarily limited to, ticarcillin, mezlocillin, piperacillin, and the like.

The quinolone-based antimicrobial agent chemically has a quinolone carboxylic acid and inhibits a DNA swirling enzyme that compresses DNA into a supercoil state, thereby inhibiting DNA synthesis and exhibiting an antibacterial effect. Examples thereof include ciprofloxacin, levofloxacin levofloxacin, moxifloxacin, and the like, but are not necessarily limited thereto.

The tetracycline-based antimicrobial agent chemically has a tetracycline nucleus. It interferes with the transcription of t-RNA in the ribosome of the microorganism and affects the protein synthesis and exhibits an antibacterial effect. Examples thereof include chlortetracycline, oxytetracycline, Oxytetracycline, doxycyclin, and the like, but are not necessarily limited thereto.

The cephalosporin-based antimicrobial agent chemically has a beta-lactam ring and inhibits bacterial cell wall formation and exhibits an antibacterial effect. Examples thereof include cefuroxime, cefaclor, ceftriaxone, (cefotaxim), but are not necessarily limited thereto.

The above-mentioned macrolide-based antibacterial agent chemically has a form in which a sugar is attached to a lactone ring and binds to a ribosome to inhibit protein synthesis, thereby exhibiting an antibacterial effect. Examples thereof include erythromycin, spiramycin, tylosin ), But are not necessarily limited thereto.

When the conventional antimicrobial agent is further included in the composition, the antimicrobial activity with different mechanisms may be further enhanced, and the antimicrobial activity may be further enhanced against various kinds of bacteria.

The content of the conventional antimicrobial agent is in the range of 0.2 to 1.2, preferably 0.3 to 1.0, based on the weight of the plant extract or the fraction thereof. If the content of the antimicrobial agent is less than 0.2, the synergy or the addition effect may be insignificant. If the content of the antimicrobial agent is more than 1.2, the antimicrobial agent may precipitate or be uneconomical to the effect.

In one specific embodiment, the antimicrobial activity against the methicillin-resistant Staphylococcus aureus and the methicillin-sensitive Staphylococcus aureus of the ethanol extract or fraction thereof in the above-ground part of the mugwort bug was observed using each extract or fraction alone Showed a slight antimicrobial activity in the majority of methicillin-resistant Staphylococcus aureus and methicillin-susceptible Staphylococcus aureus. However, synergistic effect was observed when used in combination with ampicillin. In the case of using it in combination with oxacillin, methicillin- For the streptococci, an additive effect, and for methicillin-susceptible Staphylococcus aureus, a synergistic effect.

In another specific embodiment, the antimicrobial activity against the methicillin-resistant Staphylococcus aureus and the methicillin-sensitive Staphylococcus aureus of each of the ethanol extracts or fractions thereof was examined. As a result, it was found that the antioxidative activity of the ethanol extract of the yezo shoots and the normal hexane fraction And when used in combination with ampicillin or oxacillin, had an additional effect on methicillin-resistant Staphylococcus aureus.

In another specific embodiment, the antimicrobial activity of lanes against methicillin-resistant Staphylococcus aureus and methicillin-sensitive Staphylococcus aureus was observed. As a result, antimicrobial activity was observed even when used alone, but it was used in combination with ampicillin or oxacillin In the case of methicillin-susceptible Staphylococcus aureus, the synergistic effect was shown, whereas for methicillin-resistant Staphylococcus aureus, synergy was generally observed.

In another specific embodiment, the antimicrobial activity against methicillin-resistant Staphylococcus aureus and methicillin-sensitive Staphylococcus aureus was examined in combination with ampicillin or oxacillin, respectively. Synergistic effects were seen in both methicillin-resistant Staphylococcus aureus and methicillin-susceptible Staphylococcus aureus.

In another specific embodiment, the antimicrobial activity against the methicillin-resistant Staphylococcus aureus and the methicillin-sensitive Staphylococcus aureus of each of the ethanol extracts of the miso paste or its fractions was observed. As a result, the addition of methicillin-resistant Staphylococcus aureus However, synergistic effects were observed for methicillin-susceptible Staphylococcus aureus.

In another specific embodiment, the antimicrobial activity of each of the extracts of ethanol or its fractions against methicillin-resistant Staphylococcus aureus and methicillin-sensitive Staphylococcus aureus was examined. As a result, when each extract or fraction was used alone Although methicillin-resistant Staphylococcus aureus showed a slight antimicrobial activity, there was additional effect when used in combination with ampicillin. When used in combination with oxacillin, both methicillin-resistant Staphylococcus aureus and methicillin-susceptible Staphylococcus aureus The synergy effect was shown.

Therefore, by using a conventional antimicrobial agent in the plant extract or fraction, an antibacterial activity equal to or higher than that of conventional antimicrobial agents can be obtained while using a smaller amount of conventional antimicrobial agents. Therefore, it is economical and minimizes adverse effects, .

The antimicrobial composition of the present invention can be used for various purposes for antibacterial purposes, examples of which include medicines, detergents, and cosmetics.

In one specific embodiment, the antimicrobial composition is used as an antimicrobial pharmaceutical composition.

The antimicrobial composition of the present invention contained in the pharmaceutical composition is 0.0001 to 30% by weight, preferably 0.0001 to 10% by weight based on the total weight of the pharmaceutical composition.

The pharmaceutical composition may be formulated into tablets, capsules, powders, granules, suspensions, emulsions, syrups, emulsions, warning agents, ointments, spraying agents , Oil, gel, main tablet, tincture, bath, liniments, lotions, patches, pads, creams and the like. In addition, it can be preferably used as an external preparation for skin intended for topical administration for application directly to the affected area. In this case, a form such as an ointment, a lotion, a spray or a gel is preferable. These external preparations for skin may also be included in a support base or matrix or the like, which can be applied directly to the treatment site, and examples of the support substrate include gauze or bandages. The plant extracts or fractions thereof used in the formulation may be in the form of a colloid or a dried powder.

When formulating with ointment, consideration should be given to skin surface temperature, skin pH, transdermal water loss value, total lipid value of epidermis, etc., and vaseline, liquid paraffin, paraffin, plastic base, silicone, a water-soluble base, an emulsifiable base, a suspension base, and the like, such as vegetable oil, lard, wax, and purified lanolin. Such ointments may be used in combination with antioxidants such as tocopherol, BHA, BHT, NDGA, etc., preservatives such as phenolic substances, chlorobutanol, benzyl alcohol, parabens and benzoic acid, humectants such as glycerin, propylene glycol, sorbitol ), A dissolution aid (e.g., ethanol, propylene glycol), a softening aid (e.g., liquid paraffin, glycerin, propylene glycol, an interfacial surface agent, etc.).

When the composition is formulated into a lotion, it may be prepared as a lotion such as a solution, a suspension or an emulsion. In the case of a lotion applied to the skin, it may be manufactured to have a viscosity of, for example, 200 cps to 500 cps. A wetting agent such as glycerin or propylene glycol is preferably blended.

When formulated as a sprayer, a propellant or the like may be formulated with the additive such that the water-dispersed concentrate or wet powder is dispersed.

When formulated as a patch, an absorption enhancer can be used to increase absorption of the compound through the skin.

The pharmaceutical composition of the present invention may be administered by various administration methods such as oral, parenteral, topical, rectal, intranasal, and the like. In the case of oral administration, for example, oral solid preparations may be formulated with a diluent (e.g., lactose, dextrose, sucrose, cellulose, cornstarch, potato starch, etc.), a lubricant (e.g., silica, talc, , Starch, arabic gum, gelatin methyl cellulose, carboxymethyl cellulose, polyvinyl pyrrolidone, etc.), disintegrating agents (e.g., starch, alginic acid, alginate, Sodium starch glycolate, and the like), formaldehyde, dyes, sweeteners, wetting agents (e.g., lecithin, polysorbate, lauryl sulfate, etc.), and other pharmacologically inert materials commonly used in pharmaceuticals and the like. For topical administration, excipients such as excipients such as lactose, starch, cellulose, lactose, polyethylene glycol, lubricants such as magnesium stearate, stearic acid, glyceryl behenate, talc, Aluminium chloride, etc.) and the like.

Parenteral administration includes injections that result in systemic effects or injections that are administered directly to the affected area. Examples of parenteral administration include subcutaneous, intravenous, intramuscular, intradermal, intrathecal, guiding and general injection techniques.

Topical administration includes the treatment of an affected area or organ that is readily accessible by topical application, such as ear, vaginal, open and sealed wound or closed wound and skin, including eye, ear and middle ear infections. It also includes transdermal delivery, which leads to systemic effects.

Rectal administration includes the form of suppositories.

Intranasal administration includes co aerosol or inhalation application.

The pharmaceutical composition of the present invention is administered in a pharmaceutically effective amount. In the present invention, a pharmaceutically effective amount means an amount sufficient to treat or prevent a disease at a reasonable benefit / risk ratio applicable to medical treatment or prevention. The effective dose level will depend on the type of disease and its severity, Including the age, weight, health and sex of the patient, sensitivity of the patient to the drug, time of administration of the particular extract used, route of administration and rate of release, duration of treatment, Can be determined according to factors well known in the art. Generally, an adult dose of 0.1 to 1000 mg / kg per day, preferably 10 to 100 mg / kg per day, may be administered once to several times per day.

The present invention also relates to a pharmaceutical composition comprising the antibacterial pharmaceutical composition alone or a composition containing the antibacterial agent in addition to the antibacterial composition to provide a bacterium, a bacterium or a spiral bacterium, particularly methicillin-susceptible Staphylococcus aureus, The present invention relates to a method for treating Staphylococcus aureus.

Such treatment refers to therapeutic treatment and prevention or prevention measures. Therefore, those who need treatment include not only those infected with the above-mentioned bacteria, but also those who want to prevent infection with the above-mentioned bacteria. The term " animal " refers to all animals that are infected with the fungus and require treatment, and specifically include mammals such as humans, cows, dogs, horses and pigs, rodents such as guinea pigs, hamsters, squirrels, and rats, But are not limited to, reptiles such as birds, snakes, lizards, turtles and crocodiles, amphibians such as frogs and salamanders.

In another specific embodiment, the antimicrobial composition is used as an antimicrobial detergent composition.

The detergent refers to materials and products that can build up, remove, or disperse solids and liquid dirt from the surface being cleaned, and the cleaning refers to the removal of unwanted materials or microorganisms from the surface.

The antimicrobial composition contained in the cleaning agent of the present invention is 0.0001 to 30% by weight, preferably 0.0001 to 20% by weight, and most preferably 0.0001 to 10% by weight based on the total weight of the cleaning agent.

The components contained in the cleaning agent of the present invention include, as an active ingredient, components commonly used in detergents in addition to the antimicrobial composition described above. For example, conventional adjuvants such as perfumes, dyes, solvents, opacifying agents, copolymerization system dispersions, thickening agents, cellulose derivatives, krypton gum, albumin derivatives, fats, oils, keratolytic agents, herbicides, thickeners, stabilizers, Carriers and the like.

The detergent of the present invention can be used as a detergent for cleaning the living environment such as a detergent for washing human body such as shampoo, rinse, soap, hand wash, foam cleansing, a dishware, a bathroom, a sink, But is not limited thereto.

The detergent of the present invention can be prepared into any formulation conventionally produced in the art. For example, liquids, solids, creams, and spray agents.

In another specific embodiment, the antimicrobial composition is used as an antimicrobial cosmetic composition.

The antimicrobial composition contained in the cosmetic of the present invention is 0.0001 to 20% by weight, preferably 0.0001 to 10% by weight, and most preferably 0.0001 to 3.0% by weight based on the total weight of the cosmetic.

The ingredients contained in the cosmetic of the present invention include, as an active ingredient, the ingredients commonly used in cosmetics in addition to the antimicrobial composition described above. Such as thickeners, stabilizers, solubilizers, vitamins, customary adjuvants such as pigments and perfumes, and carriers and the like.

The cosmetic composition of the present invention can be prepared into any formulation conventionally produced in the art. For example, they may be formulated as solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations and sprays, But is not limited thereto. More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the cosmetic formulation of the present invention is a paste, a cream or a gel, the carrier component may be an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracer, a cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, calcium silicate Or polyamide powder may be used, and in the case of spray, additionally, propellants such as chlorofluorohydrocarbons, propane / butane or dimethyl ether may be used.

When the cosmetic formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid esters of sorbitan.

When the cosmetic formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the cosmetic preparation of the present invention is a cleansing agent containing an interfacial active agent, it is preferable to use, as the carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, Amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester.

In another embodiment, the present invention relates to a method for producing an antimicrobial composition, which comprises the step of producing any one or more of the above-mentioned plant extracts or fractions thereof, or a step of further containing a conventional antimicrobial agent in the above extract or fraction ≪ / RTI >

In the method for producing the antimicrobial composition of the present invention, the first step is a step of producing a plant extract or a fraction thereof. The plant may be any one or more selected from the group consisting of mulberry leaves, yuliai, lane, ginseng root, daphnia magna, miso paste, and branches, and the contents of the extracts or fractions thereof are described above.

In one specific embodiment, plants are prepared using ethanol and then sequentially fractionated using normal hexane, ethyl acetate, normal butanol and water.

The next step is a step further comprising a conventional antimicrobial agent in the plant extract or fraction thereof. When the above-mentioned plant extract or fraction thereof is further used with a conventional antimicrobial agent, the plant extract or fractions thereof having a different mechanism exhibit synergistic or additive effect with the antimicrobial agent, so that even when a small amount of antimicrobial agent is used, It is economical, safe and can exhibit antibacterial activity in a wide variety of bacteria. For the contents and content of the antimicrobial agent, refer to the above description.

The above-mentioned plants are natural products which have been used for a long time by folk remedies and have little toxicity and side effects of their own, so they can be used in medicines, detergents and cosmetics without worrying about toxicity and side effects.

Since the antimicrobial composition of the present invention uses various plants which are natural products, there is little concern about side effects, is safe, and has excellent antimicrobial activity, so that it can be usefully used in medicines, cleaning agents or cosmetics. In particular, it exhibits an excellent antimicrobial activity against methicillin-resistant Staphylococcus aureus, as well as methicillin-resistant Staphylococcus aureus. Especially, when a conventional antimicrobial agent is further contained, synergistic effect is exhibited. That is, even if a small amount of the antimicrobial agent is used, the antimicrobial activity of the antimicrobial agent can be equal to or higher than that of the conventional antimicrobial agent, so that the risk of side effects caused by the abuse of the antimicrobial agent is low, economical and efficient. In addition, since plants that can be easily found in Korea are used, they can be recycled and high value-added products can be sought.

Hereinafter, the present invention will be described in more detail with reference to examples. It is to be understood by those skilled in the art that these embodiments are only for describing the present invention specifically and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention.

Example  1: plant extract and Fraction  Produce

1-1. Collection of plants

Mulberry juice, Yonga juice, ginseng cheonghak, gyeongjae, miso paste and branch were collected from Sunchon in June, 2010. Lane was purchased from Rhubarb. Samples were confirmed by Professor Shin Dong - Won of the Department of Oriental Medicine and Medicine, Sunchon National University.

1-2. Plant Extract and Fraction Production

The plants collected in Example 1-1 were dried and boiled for 3 hours in 1 L of ethanol. Next, the ethanol extract was dissolved in distilled water at a concentration of 10%, and then fractionated in the order of n-hexane, ethyl acetate (EtOAc), normal butanol (n-BuOH) 4 < / RTI >

Example  2: Collection and culture method of strain

2-1. Collection of strains

Clinical isolates (DPS1-14) for methicillin resistant Staphylococcus aureus (MRSA) were isolated from 14 patients at Wonkwang University Hospital. The methicillin-resistant strains (methicillin-resistant strain) of S. aureus ATCC 33591 and methicillin-susceptible strains (methicillin-susceptible strain) S. aureus ATCC 25923 was purchased from American Type Culture Collection (ATCC), USA.

2-2. Storage and culture method of strain

The fungi of Example 2-1 were stored in 30% glycerol and then stored frozen at -70 ° C. The fungi were cultured using Mueller-Hinton broth (MHB) and Mueller-Hinton agar (MHA). When cultured in Mueller-Hinton medium, Were suspended in a Mueller-Hinton medium and cultured at 37 DEG C for 24 hours.

Example  3: mugwort extract or Fraction  Antimicrobial activity measurement

3-1. Determination of minimum inhibitory concentration

(1) Preparation of sample

Samples were prepared so that the initial concentration of the ethanol extract, the normal hexane fraction, the ethyl acetate fraction, the normal butanol fraction, the water fraction, the ampicillin and the oxacillin of the ethanol extract,

(2) Determination of minimal inhibitory concentration (MIC)

The minimum inhibitory concentration was determined by the CLSI microdilution broth method. Methicillin-susceptible strains S. aureus ATCC 25923, methicillin resistant strain S. aureus ATCC 33591, and clinical isolates DPS1 to DPS14 for methicillin-resistant Staphylococcus aureus at a concentration of 1.5 x 10 ⁸ CFU / ml. The sample (1) of Example 3-1 prepared at a concentration of 2000 / / ml was sequentially diluted twice in 50% DMSO to give a concentration of the sample ranging from 2000 / / ㎖ to 0.975 / / ㎖ . Then, 100 μl of the liquid medium, 10 μl of the bacterial solution and 10 μl of the sample of each concentration were treated in each well of the 96-well plate and cultured at 37 ° C for 18 hours. The minimum inhibitory concentration was determined by the methicillin susceptibility strain S. aureus ATCC 25923, methicillin-resistant S. aureus ATCC 33591 and methicillin-resistant Staphylococcus aureus strains DPS1 to DPS14 were completely inhibited, and the results obtained from the experiments are shown in Tables 1 and 2 below. Table 2 shows the results.

As a result, as shown in Table 1, the ethanol extract and the normal hexane fraction of the ethanol extract on the surface of the mugwort pupa showed a weak antibacterial activity against methicillin-resistant Staphylococcus aureus ATCC 33591, but compared to ampicillin and oxacillin The antimicrobial effect did not appear to be significant.

In addition, as shown in Table 2, when the test strain of the methicillin-resistant Staphylococcus aureus of the normal hexane fraction against the ethanol extract of the above-ground ethanol extract was increased, the methicillin-resistant yellow staphylococcus aureus of the normal hexane fraction in the above- The antimicrobial activity against the streptococci was lower in the experimental group than in the ampicillin. In addition, methicillin-susceptible Staphylococcus aureus had a weaker antibacterial activity than ampicillin or oxacillin.

3-2. Determination of growth inhibition degree

(1) Preparation of strain suspension

In order to measure the antimicrobial activity against the normal hexane fraction of the mugwort ethanol extract by the disc diffusion method, S. aureus ATCC 25923, methicillin-resistant strain S. aureus ATCC 33591, and methicillin-resistant yellow Clinical isolates DPS1 to DPS14 for Staphylococci were cultured in Mueller-Hinton agar medium for 24 hours at 37 占 폚. Thereafter, the strains were inoculated on a Mueller-Hinton medium to prepare a suspension having a turbidity of about 1.5 × 10 ⁸ CFU / ml.

(2) Preparation of sample

The normal hexane fractions, ampicillin and oxacillin of the ethanol extracts from the above-ground parts of the mugwort were prepared so that the initial concentration was 2000 ug / ml.

(3) Inoculation and culture of samples

* The Mueller-Hinton agar broth was poured into a Petri dish, and 100 μl of the suspension prepared in (1) of Example 3-2 was added to each Petri dish and plated. Thereafter, a sterilized paper disk having a diameter of 6 mm was placed in the center of the petri dish. Next, the normal hexane fraction of the ethanol extract of the above-mentioned ground warts prepared in Example 3-2 (2) was sequentially diluted 2 times in each of the Petri dishes with a paper disk and 10 μl of each sample was loaded, ≪ / RTI > The Petri dishes were then incubated in a plant growth chamber at 37 DEG C for 18 hours.

(4) Measurement of antimicrobial activity

The antibacterial activity was measured by measuring the diameter at which the growth of the strain was inhibited around each disk after the incubation time of (3) of Example 3-2. The results obtained from the experiment are shown in Table 3 below.

As a result, the fraction of normal hexane in the upper part of the mugwort was not antimicrobially effective compared to ampicillin, although both methicillin-resistant Staphylococcus aureus and methicillin-sensitive Staphylococcus aureus showed antimicrobial activity.

In addition, the inhibition of strain growth was observed in 500ug of the normal hexane fraction, which was dependent on the concentration of the normal hexane fraction.

3-3. Determination of split inhibition concentration index

(1) Preparation of sample

The normal hexane fractions, ampicillin and oxacillin of the ethanol extracts from the above-ground parts of the mugwort were prepared so that the initial concentration was 2000 ug / ml.

(2) Determination of partition inhibition concentration index

In order to examine the effect of the combination of the normal hexane fraction in the upper part of the mugwort, and the effect of the combination of the ampicillin and the oxacillin, the fraction of normal hexane, ampicillin or oxacillin in the upper part of the mugwort, The minimum inhibitory concentration in the case of further treatment with ampicillin or oxacillin in the presence of the normal hexane fraction in the upper part of the mugwort, and in the case of further treatment with the normal hexane fraction in the upper part of the mugwort, in the presence of the ampicillin or oxacillin, was determined .

As shown in the following empirical formula, fractional inhibitory concentration inhibition (FICI) was calculated by dividing the minimum inhibitory concentration of each sample by the minimum inhibitory concentration when treated alone (FICI MIC (A), where MIC (AB) is the minimum inhibitory concentration for further treatment of substance A with B substance, MIC (A) The minimum inhibitory concentration of substance A, MIC (BA), is the minimum inhibitory concentration when B substance is further treated when A substance is present, MICA (B) means the minimum inhibitory concentration of substance B, and the FICI value is 0.5 or less If synergistic effect is less than 0.5 and less than 1.0, it means that there is an additional effect.) The results are shown in Tables 4 and 5 below.

The results showed that the inhibitory concentration index of normal hexane fractions and amphycillin in the upper part of the mugwort was lower than 0.5 in both methicillin - resistant Staphylococcus aureus and methicillin - susceptible Staphylococcus aureus.

However, the inhibitory concentration index of the fraction of normal hexane and oxacillin in the upper part of the mugwort was lower than that of methicillin-susceptible Staphylococcus aureus (0.06), whereas in methicillin-resistant Staphylococcus aureus was 0.5 and 1.0 ) Effect was observed.

Example  4 : Yonga  Extract or Fraction  Antimicrobial activity measurement

4-1. Determination of minimum inhibitory concentration

In order to determine the minimum inhibitory concentrations of the ethanol extracts of the yellowness supernatant and the minimum inhibitory concentrations of the yellowness supernatant extracts on the normal hexane fraction, the ethyl acetate fraction, the normal butanol fraction, the water fraction, the ampicillin and the oxacillin, 1, the results are shown in Tables 6 to 8. < tb >< TABLE >

As a result of the experiment, it was observed that the ethanol extract of yezoensis root had the best antimicrobial activity against methicillin-resistant Staphylococcus aureus, as shown in Table 6 above.

As can be seen in Table 7, the fraction of normal hexane among the fractions of the ethanol extract of yezoensis root showed excellent antimicrobial activity in both methicillin-resistant Staphylococcus aureus and methicillin-sensitive Staphylococcus aureus.

In addition, as shown in Table 8 above, even when the experimental group of the antimicrobial effect of the fraction of xanthohumbo n-hexane was experimentally increased, the fraction of normal hexane in the root of xanthan gum was superior to those of methicillin-resistant Staphylococcus aureus and methicillin-susceptible Staphylococcus aureus And showed excellent antimicrobial activity against methicillin-resistant Staphylococcus aureus compared with the control ampicillin or oxacillin.

4-2. Determination of growth inhibition degree

The results obtained by performing the experiment by the method of Example 3-2 in order to determine the degree of inhibition of the normal hexane fraction, the ampicillin and the oxacillin against the ethanol extract of the root of yezoacorrhoeae are shown in Table 9 below.

As a result, the fraction of normal hexane in the root of Yong-saek showed antimicrobial activity against methicillin-resistant Staphylococcus aureus and methicillin-susceptible Staphylococcus aureus. Especially, methicillin-resistant Staphylococcus aureus had antibacterial effect against ampicillin or oxacillin .

In addition, when the strain was loaded at a loading of 500 ug compared to the case of loading 250 ug of the normal hexane fraction of the elongated root, the inhibition diameter of the strain was observed to be broad and the concentration dependent antimicrobial activity was shown in all the tested groups.

4-3. Determination of split inhibition concentration index

The results obtained by carrying out the experiment by the method of Example 3-3 above are shown in Tables 10 and 11 to see the effect of the combination of the normal hexane fraction of yezo super roots and the ampicillin or oxacillin.

As a result of the experiment, the fractional inhibitory concentration index of the normal hexane fraction of the root of yezoensis and the concentration of ampicillin or oxacillin was 0.5 to 1.0, showing an additive effect.

Example  5: Lane ( Rhein ) Of antimicrobial activity

5-1. Determination of minimum inhibitory concentration

Lane, ampicillin, and oxacillin. The results are shown in Table 12 below. ≪ tb >< TABLE >

As a result, the lanes were found to have antimicrobial activity against both methicillin-resistant Staphylococcus aureus and methicillin-sensitive Staphylococcus aureus. Especially, methicillin-resistant Staphylococcus aureus had excellent antimicrobial activity against ampicillin or oxacillin .

5-2. Determination of growth inhibition degree

Lane, ampicillin, and oxacillin. The results are shown in Table 13 below. ≪ tb >< TABLE >

Experimental results showed that lanes showed antimicrobial activity against both methicillin-resistant Staphylococcus aureus and methicillin-susceptible Staphylococcus aureus, and methicillin-resistant Staphylococcus aureus was superior to ampicillin and oxacillin.

In addition, in all the experimental groups, when the strain was loaded at a loading of 500 ug compared to the case of loading 250 ug of the lane, the inhibited diameter of the strain was observed to be broad and the concentration dependent antimicrobial activity was exhibited.

5-3. Determination of split inhibition concentration index

The results obtained by carrying out the experiment by the method of Example 3-3 are shown in Tables 14 and 15 below in order to see the combined effect of lane and ampicillin or oxacillin.

As a result, the inhibitory concentration index of lanes and ampicillin or oxacillin was 0.5 or less in methicillin-sensitive Staphylococcus aureus, and it was observed that methicillin-resistant Staphylococcus aureus had a synergistic effect, The effect was observed.

Example  6: Gamshin  Antimicrobial activity measurement

6-1. Determination of minimum inhibitory concentration

In order to determine the minimum inhibitory concentrations of the ethanol extract of ginseng, the ethanol extract, the normal hexane fraction, the ethyl acetate fraction, the normal butanol fraction, the water fraction, the ampicillin and the oxacillin, The results are shown in Tables 16 and 17 below.

As shown in Table 16, the antimicrobial activity against the methicillin-resistant Staphylococcus aureus and the methicillin-susceptible Staphylococcus aureus of the ethanol extract of Ganoderma lucidum was observed to be weak.

As can be seen in Table 17, the fractions of normal hexane among the various fractions of ganoderma lucidum showed the highest antimicrobial activity against methicillin-resistant Staphylococcus aureus and methicillin-sensitive Staphylococcus aureus, Ampicillin. ≪ / RTI >

6-2. Determination of growth inhibition degree

The results obtained by performing the experiment by the method of Example 3-2 in order to determine the degree of inhibition of the ethanol extract of ginseng, ethanol fraction, normal hexane fraction, ampicillin and oxacillin are shown in Table 18 below.

As a result of the experiment, the ethanol extract of Gamsohyang and the fraction of normal hexane showed antimicrobial activity in both methicillin resistant Staphylococcus aureus and methicillin susceptible Staphylococcus aureus. In the case of methicillin - susceptible Staphylococcus aureus, both the ethanol extract of ginseng and the normal hexane fraction showed superior antimicrobial activity over that of ampicillin. However, the antimicrobial activity of methicillin - resistant Staphylococcus aureus was weaker than that of ampicillin.

In addition, when the strain was loaded at a loading of 500 ug compared to the case of loading 250 ug of the ethanol extract and the normal hexane fraction of ginseng, the inhibition of the growth of the strain was observed widely in all the experimental groups, showing a concentration dependent antimicrobial activity.

6-3. Determination of split inhibition concentration index

The results obtained by performing the experiment using the method of Example 3-3 to examine the combined effect of the normal hexane fraction of ginseng and amphycillin or oxacillin are shown in Tables 19 and 20 below.

The results showed that the inhibitory concentration indices of the fraction of normal hexane and the concentration of ampicillin or oxacillin in the ginseng root were 0.5 or less in both methicillin - resistant Staphylococcus aureus and methicillin - susceptible Staphylococcus aureus.

Example  7: Antimicrobial activity measurement

7-1. Determination of minimum inhibitory concentration

In order to determine the minimum inhibitory concentrations of the ethanol extracts of the anthelmintics, the ethanol extracts, the normal hexane fractions, the ethyl acetate fractions, the normal butanol fractions, the water fractions, the ampicillins and the oxacillin, Are shown in Tables 21 and 22 below.

As a result, both the ethanol extracts of N. meningitidis and the normal hexane fraction showed antimicrobial activity against methicillin-resistant Staphylococcus aureus and methicillin-sensitive Staphylococcus aureus, and the degree of antimicrobial activity was superior to that of the control strains of ampicillin and oxacillin.

7-2. Determination of growth inhibition degree

The results obtained by performing the experiment by the method of Example 3-2 in order to determine the degree of inhibition of the normal hexane fraction, the ampicillin and the oxacillin against the ethanol extract of the larvae are shown in Table 23 below.

As a result, the fraction of normal hexane in the genus Staphylococcus showed excellent antimicrobial activity against methicillin-susceptible Staphylococcus aureus compared with ampicillin and oxacillin, but the antimicrobial activity of methicillin-resistant Staphylococcus aureus was equal to or slightly weaker than that of ampicillin Respectively.

In addition, when the strain was loaded at a loading of 500 ug as compared with the case of loading 250 ug of the normal hexane fraction in the experimental groups, the diameter of the inhibition of the growth of the strain was widely observed, showing a concentration-dependent antimicrobial activity.

7-3. Determination of split inhibition concentration index

The results obtained by performing the experiment using the method of Example 3-3 to see the effect of the combination of the normal hexane fraction and the ampicillin or the oxacillin of the larvae are shown in Tables 24 and 25 below.

The results showed that the inhibitory concentration index of the fraction of normal hexane and the concentration of ampicillin or oxacillin in both the methicillin - resistant Staphylococcus aureus and the methicillin - sensitive Staphylococcus aureus was 0.5 or less.

Example  8 : Miso paste  Antimicrobial activity measurement

8-1. Determination of minimum inhibitory concentration

In order to determine the minimum inhibitory concentration of the ethanol extract of the soybean paste patch and the ethanol extract of the soybean paste leaf leaf extract against the normal hexane fraction, the ethyl acetate fraction, the normal butanol fraction, the water fraction, the ampicillin and the oxacillin, The results are shown in Tables 26 and 27 below.

As shown in Table 26, the antimicrobial activity of the ethanol extract on the leaves of the soybean paste was highest in both methicillin-resistant Staphylococcus aureus and methicillin-susceptible Staphylococcus aureus.

In addition, the fraction of normal hexane fractions of the fraction of ethanol extract of soybean paste leaf showed the best antimicrobial activity in both methicillin resistant Staphylococcus aureus and methicillin susceptible Staphylococcus aureus, but the degree was not so good.

8-2. Determination of growth inhibition degree

The results obtained by performing the experiment using the method of Example 3-2 to determine the degree of inhibition of growth of the normal hexane fraction, the ampicillin and the oxacillin against the ethanol extract and the ethanol extract of the soybean paste leaf are shown in the following Table 28.

As a result, the fraction of normal hexane in soybean paste leaf showed antimicrobial activity against methicillin - resistant Staphylococcus aureus and methicillin - susceptible Staphylococcus aureus, but the antimicrobial effect was generally weaker than that of the control ampicillin.

In addition, in all the experimental groups, when the strain was loaded at a loading of 500 ug compared to the case of loading 250 ug of the normal hexane fraction of the soybean paste leaf, the inhibited diameter of the strain was observed to be broad and the concentration dependent antimicrobial activity was exhibited.

8-3. Determination of split inhibition concentration index

The results obtained by performing the experiment using the method of Example 3-3 to see the effect of the combination of the normal hexane fraction and the ampicillin or oxacillin in the soybean paste leaves are shown in Tables 29 and 30 below.

As a result of the experiment, it was observed that the fractional inhibitory concentration index of the normal hexane fraction and the concentration of ampicillin or oxacillin in the miso leaves of methicillin resistant Staphylococcus aureus was 1.0, which is an additive effect. However, the fractional inhibitory concentration index of the normal hexane fraction and the concentration of ampicillin or oxacillin in the miso leaves of methicillin - susceptible Staphylococcus aureus was observed to be synergistic below 0.5.

Example  9: Antimicrobial activity measurement

9-1. Determination of minimum inhibitory concentration

The results obtained by carrying out the experiment using the method of Example 3-1 to obtain the minimum inhibitory concentration of the ethanol extract of the leaflet, the normal hexane fraction, the ethyl acetate fraction, the normal butanol fraction, the water fraction, the ampicillin and the oxacillin of the ethanol extract Are shown in Table 31 below.

The antimicrobial activity of methicillin-sensitive Staphylococcus aureus against the ethanol extract of the seedlings was weaker than that of ampicillin or oxacillin. However, the antimicrobial activity against methicillin-resistant Staphylococcus aureus was lower than that of ampicillin or oxacillin Was superior to silane.

9-2. Determination of growth inhibition degree

The results obtained by performing the experiment by the method of Example 3-2 in order to determine the growth inhibition degree of the normal hexane fraction, the ampicillin and the oxacillin against the ethanol extract of the seedlings are shown in Table 32 below.

As a result, the fraction of normal hexane in the fungus showed antimicrobial activity against both methicillin-resistant Staphylococcus aureus and methicillin-susceptible Staphylococcus aureus. In particular, the antimicrobial activity against methicillin-resistant Staphylococcus aureus was superior to that of ampicillin or oxacillin Respectively.

In addition, in all the experimental groups, when the strain was loaded at a loading of 500 ug compared to 250 ug of the normal hexane fraction, the growth inhibition diameter of the strain was widely observed, indicating a concentration-dependent antimicrobial activity.

9-3. Determination of split inhibition concentration index

The results obtained by carrying out the experiment by the method of Example 3-3 to see the effect of the combination of the normal hexane fraction with the ampicillin or the oxacillin of the seedlings are shown in the following Tables 33 and 34.

As a result, the inhibitory concentration index of amylase or oxycillin was lower than 0.5 in methicillin - susceptible Staphylococcus aureus. In addition, the fractional inhibitory concentration index of the normal hexane fraction and the ampicillin for the methicillin resistant Staphylococcus aureus strain was different depending on the group, but it was observed to have an additive effect in general. The fraction of normal hexane and the partition inhibition concentration of oxacillin Was 0.5 or less, and it was observed that there was a synergy effect.

Example  10: Acute Oral Toxicity Test

In order to confirm that the plant extract of the present invention is not toxic, an acute oral toxicity test was conducted. Twenty SPF SD rats aged 5 to 6 weeks as an experimental animal were used for an acute toxicity test under the following conditions.

Temperature and Humidity Conditions: Temperature 22 degrees +/- 2 degrees, relative humidity 50 +/- 10%

Contrast Cycle: Fluorescent lighting (Turns off at 8 o'clock then turns off at 20 o'clock)

Illumination: 200 to 300 lux

UV-treated drinking water is free.

The test substance was diluted in sterilized distilled water to prepare a test sample. The same amount of sterilized distilled water was used as a control.

On the day of administration, the general condition was observed every hour up to 4 hours, and one day from the next day until the 14th day, careful observation of changes in general condition, poisoning symptoms, motility, appearance, autonomic nerves and dead animals, The pathological anatomical examination was performed to determine the presence or absence of toxicity. As a result, the LD ₅₀ was 3000 mg / kg BW and no toxicity was observed.

Claims (10)

Which comprises as an active ingredient a soybean paste extract or a fraction thereof.
2. The antimicrobial pharmaceutical composition according to claim 1, wherein the composition further comprises ampicillin or oxacillin.
delete The antimicrobial pharmaceutical composition according to claim 1, wherein the bacterium is at least one selected from the group consisting of bacillus, staphylococci, and staphylococci.
5. The antimicrobial pharmaceutical composition according to claim 4, wherein the bacterium is at least one selected from the group consisting of Staphylococcus aureus, Staphylococcus epidermidis and vancomycin-mediated Staphylococcus aureus.
The pharmaceutical composition for antimicrobial use according to claim 1, wherein the extract is extracted with at least one solvent selected from the group consisting of methanol, ethanol, propanol, isopropanol, butanol, pentanol and hexanol.
2. The antimicrobial pharmaceutical composition according to claim 1, wherein the fraction is fractionated into at least one selected solvent selected from the group consisting of n-hexane, ethyl acetate, n-butanol and water.
An antimicrobial detergent containing as an active ingredient a soybean paste extract or a fraction thereof.
9. The antimicrobial detergent according to claim 8, wherein the detergent further comprises ampicillin or oxacillin.
The antimicrobial detergent according to claim 8, wherein the bacterium is at least one selected from the group consisting of bacillus, staphylococci, and staphylococci.