KR101468819B1 - Antibacterial compositions containing Ficus carica L extracts or fractions thereof under light intensity - Google Patents

Abstract

The present invention relates to a method for the production of one or more selected from the group consisting of rice terra cotta bed, paulownia tree, witch hazel, fig tree, chestnut, Indian potato, Which comprises irradiating a plant extract or a fraction thereof with light as an effective ingredient. The antimicrobial composition prepared by using the plant of the present invention has an antimicrobial activity, particularly, the antimicrobial activity against methicillin-resistant Staphylococcus aureus, which is the main infectious disease in a hospital. In addition, since the antimicrobial activity is enhanced when the plant extract or its fractions are irradiated with light, when controlling the intensity and irradiation time of the irradiated light, an antimicrobial agent exhibiting an antimicrobial activity equal to or more than that of existing antimicrobial agents It is safe and economical to manufacture. In addition, since plants that can be easily found in Korea are used, they can be recycled and high value-added products can be sought.

Description

FIELD OF THE INVENTION [0001] The present invention relates to an antimicrobial composition containing Ficus carica L extracts or fractions thereof,

The present invention relates to a method for the production of one or more selected from the group consisting of rice terra cotta bed, paulownia tree, witch hazel, fig tree, chestnut, Indian potato, Which comprises irradiating a plant extract or a fraction thereof with light.

The term "antimicrobial agent" refers to a substance that has an antibacterial action against bacteria, specifically, a substance that has antimicrobial activity by inhibiting a system in which a bacterium synthesizes cell walls or proteins or the like. Antimicrobial agents are widely used for the treatment of diseases caused by bacterial infections and the like, and they have been produced by separation from natural materials including fungi or by chemical synthesis.

The most common antimicrobial agent is penicillin discovered by Allen Sander Fleming in 1928. Penicillin is the first antimicrobial agent developed by mankind. Part of penicillin beta-lactam binds to transpeptidase, which connects peptidoglycan molecules, which are bacterial cell membranes, to prevent bacterial cell wall growth. It does not withstand the osmotic pressure, it causes the bacteria to lyse, inhibiting the growth of the bacteria and giving the antibacterial effect. Since the development of penicillin, excellent antibacterial agents have been developed on the basis of this, and a representative example is methicillin which is made by partially changing the chemical structure of penicillin.

Antimicrobial agents played many roles in preventing or treating bacterial infections, but they were resistant to conventional antimicrobial agents due to indiscriminate use of antimicrobial agents, resulting in the emergence of antimicrobial resistant bacteria that are not effective. Antimicrobial-resistant bacteria refers to bacteria that do not respond to specific antimicrobial agents because they are tolerant to certain antimicrobial agents. Resistant bacteria transfer resistance to other bacteria, which causes the resistant bacteria to continue to grow. Therefore, if resistance occurs, use antimicrobial agents with stronger antimicrobial activity or other antimicrobial agents .

Among the various bacteria resistant to antimicrobial agents, methicillin resistant Staphylococcus aureus (MRSA), vancomycin resistant enterococci (VRE), vancomycin intermediate Staphylococcus aureus (VISA) ), And E. coli P157: H7 resistant to at least six antimicrobial agents.

Methicillin-resistant Staphylococcus aureus was first reported in the UK in 1961 and has been reported in various parts of the world. Methicillin-resistant Staphylococcus aureus is resistant to most antimicrobial agents as well as methicillin, There is a problem. In addition, methicillin-resistant Staphylococcus aureus is the most frequent causative agent of pathogens in hospitals, and it is serious in that infected persons or elderly people may be fatal to death.

In 2006, for example, more than 94,000 people in the US were infected with methicillin-resistant Staphylococcus aureus, of which more than 19,000 were reported to have died. In Korea, methicillin-resistant Staphylococcus aureus is an infection pathogen of the hospital, and it is reported that it is over 80% in large general hospitals.

Increased tolerance to antimicrobial agents increases economic and social problems, such as increased use of new high-priced antimicrobial agents, increased duration of treatment, increased mortality, and causing medical disputes. Therefore, development of new antimicrobial agents is required to solve these problems. However, development of a new antimicrobial agent is time-consuming and tolerant. When natural products are used, safe drugs with less side effects can be developed. This is because natural products contain new components that are not known to date, and because they are a mixture of various components, the side effects of tolerance and the like can be reduced due to the pharmacological action between the complexes.

On the other hand, the Euphorbia supina is an annual herbaceous plant belonging to the main pole and originating in North America. It grows in fields and fields, and the stem divides branches at the bottom, spreading along the ground, and 10 to 25 cm in length. Leaves are opposite, long elliptical, with reddish brown spots in the center, white laxate, and are known to have anti-cancer, detoxification, sedation, hemostasis, and diuretic effects.

Regarding pharmaceutical or cosmetic compositions using terbinafine, the Journal of the Pharmaceutical Sciences 39 (3); 260-253 (2008) discloses compounds exhibiting antioxidative and antioxidant activities of the methanol extract of the genus Porphyra and the ethyl acetate fraction thereof.

Paulownia coreana Is a deciduous broad - leaved tree belonging to the genus. The height of the paulownia tree is about 15m, and the bark of the tree is pale brown, and the line of dark brown is vertical. It is known that the papaya peel is effective for bronchopneumonia, bacterial diarrhea, acute enteritis, acute conjunctivitis, etc. The papilled leaves are called the lobes, which are known to be effective for facial flushing and pustule.

With respect to pharmaceutical or cosmetic compositions using paulownia, Korean Patent Laid-Open Publication No. 10-2009-0021021 discloses a novel compound having antioxidative activity isolated from pung wood water resistance and disclosed in Korean Patent Laid-Open No. 10-2008-0028029 Discloses a cosmetic composition using antioxidative and anti-inflammatory properties of a paulownia leaf extract. Korean Patent Registration No. 10-0502566 discloses an antimicrobial natural dye including a water extract of paulownia tree bark.

Ranunculus sceleratus is a perennial herb that grows on the water, and is sometimes called kyungryocho, swampbear, frog, bronze duck, or thrush. Arthritis, guanwasa, swelling, ulcers, and urticaria are known to be effective.

Regarding pharmaceutical or cosmetic composition using forage and forage, Korean Patent Registration No. 10-0550773 discloses a composition comprising at least one kind of active ingredient in a group consisting of Seedlings and Seeds, Seedlings, Sea-white, And a cosmetic composition for skin whitening.

The fig tree (Ficus carica L ) is a deciduous shrub belonging to the genus Phalaenopsis, originating in the western part of Asia. It is 2 ~ 4m in height. The bark is grayish white, the leaves are alternate, broad ovate, thick, surface rough, and hairs on the back. Latex is athlete's foot, boils, hemorrhoids, leaves athlete's foot or neuralgia, fruit is known to be effective in blood pressure lowering, constipation.

Regarding pharmaceutical or cosmetic composition using a fig tree, Korean Patent Registration No. 10-0999306 discloses an agent for the development of an old chicken rehabilitation using a fig lotion. Korean Patent Registration No. 10-0512285 discloses a medicinal soap composition having an antimicrobial effect and a fungus containing fig fruit or leaf extract.

칡 ( Pueraria thunbergiana ) is a vine plant belonging to the soybean family and grows in low altitude mountains throughout Korea. The stem stretches long while the other objects are rolled up, the leaves are slanted, and the edges are flat. It is said that it is effective to stop diarrhea and stop the thirst, and juice is effective for headache, thirst and insomnia.

In connection with the pharmaceutical or cosmetic composition using ointment, Korean Patent Laid-Open Publication No. 1994-0025579 discloses a composition for preventing hyperlipidemia, inhibiting cholesterol and enhancing liver function comprising catechins as active ingredients among polyphenols . Korean Patent Laid-Open No. 10-2011-0088814 discloses a composition for improving postmenopausal women's health, which has an effect of increasing estrogen concentration, reducing blood triglyceride level, and inhibiting bone mineral density loss, which contain extract as an active ingredient. Also, Korean Patent Registration No. 10-0953800 discloses a composition for preventing white mold and treating vitiligo containing Puerariae Radix as an active ingredient.

Indian potatoes ( Apios americana Medikus is a soybean plant native to North America. It is known as Apios and American AIDS. It is known to be effective for diabetes, obesity, hypertension, atopy, HIV proliferation inhibition, osteoporosis, anemia and nourishment.

Japanese Patent Laid-Open No. 2007-332092 discloses a cosmetic composition for skin whitening and antioxidation containing Indian potato extract as an active ingredient in relation to a pharmaceutical or cosmetic composition using Indian potato.

Catalpa ovata is a deciduous broad-leaved arboreous tree belonging to the jasper family. It is also known as Gao-Dong tree, and it is distributed in Korea, China and Japan. Fruit is known as a suicide, which is known to be effective for nephritis, edema, proteinuria as a diuretic, and the inner part of the tree is called a confetti, which is known to be effective for neuralgia, hepatitis, cholecystitis, jaundice and nephritis.

Regarding pharmacological or cosmetic composition using a Japanese quince tree, Korean Patent Registration No. 10-0985719 discloses a cosmetic composition for prevention and treatment of atopic dermatitis, which has an antioxidative effect including a Japanese quince extract and an asparagus extract. Korean Patent Registration No. 10-0533768 discloses an antioxidant substance isolated from open firewood. Korean Patent Registration No. 10-0421625 discloses an anti-inflammatory agent composition containing catalodia, which is isolated from open-faced wood skin, as an active ingredient. . Korean Patent Laid-Open No. 10-2010-0001484 discloses a pharmaceutical composition for treating or preventing neurodegenerative diseases caused by dopamine deficiency comprising 4,9-dihydroxy-? -Lactone isolated from Japanese wild squirrel .

Giant ( Angelica tenuissima ) is a perennial herb that grows at the foot of a deep mountain. It is said that dry roots are dried in autumn, and it is said to be effective in headache, joint pain, toothache, abdominal pain, diarrhea and eczema.

Regarding pharmaceutical preparations or cosmetic compositions using the patches, Korean Patent Laid-Open Publication No. 10-2005-0072303 discloses a hair growth inhibitory composition comprising an extract of Ganoderma lucidum and / or Omiza extract as an active ingredient, 2005-0008324 describes a pharmaceutical composition for prevention and treatment of neurological diseases such as neuronal cell protection and ischemic stroke, which contains a ganoderma extract. Korean Patent Laid-Open Publication No. 1999-0053083 discloses a cosmetic composition comprising at least one compound selected from the group consisting of ganoderma, rhubarb, bingo and sculptor, which inhibits 5α-reductase activity which can be used for acne, hair loss and seborrheic dermatitis, Discloses an antimicrobial composition for ness.

Illicium religiosum refers to the drying of the octagonal fruit, which is an evergreen plant belonging to the pseudopodophyta. It is known to be effective in diuretic action and appetite promotion, abdominal bloating and nausea relief, back pain, constipation, cystitis, acute rheumatism and the like, which is mainly used in China.

Regarding pharmaceutical or cosmetic composition using octagonal fennel, Korean Patent Laid-Open Publication No. 10-2011-0035502 discloses a hand cleansing composition having an antibacterial and germicidal effect using an extract of clove or octagonal fennel, -2011-0035501 discloses a fiber deodorant composition having an antibacterial and bactericidal effect using an extract of cloves or octagonal fennel. Korean Patent No. 10-1041444 discloses an antibacterial soap composition using an octagonal fennel. Korean Patent Laid-Open No. 10-2011-0025715 discloses an antibacterial and antiviral detergent composition to which an octagonal fennel distillation concentrate is added. . Korean Patent Laid-Open Publication No. 2003-0079492 discloses a pharmaceutical composition for preventing and treating an inflammation-related disease containing an octopus fennel extract as an active ingredient, and Korean Patent Publication No. 2003-0019049 discloses a composition containing an octopus fennel extract And a composition for preventing and treating diabetes.

Phytolacca esculenta ) is a perennial plant belonging to the Department of Origin and is the origin of China. It grows about 1m in length, leaves are slanted, both ends are narrow, and the edges are flat. Roots are known to be effective in the treatment of nephritis and diuretics.

Japanese Patent Laid-Open Publication No. 1-153612 discloses a hair promoting composition comprising a periosteal root.

Pteridium aquilinum there is . latiusculum ) is a perennial plant belonging to the fern family. Its rootstock is more than 1m and grows in the ground. The leaf has a large triangular shape. Dried root canals are effective against parasites and are also known to be effective against bronchitis.

Korean Patent Registration No. 10-0483338 discloses a composition containing an extract of Leptodactylthus sinensis having an antimicrobial activity and an antioxidative effect in relation to a pharmaceutical or cosmetic composition using a bracken, As an active ingredient, a composition for external application for skin which can be used for anti-aging, whitening, and improvement of atopic skin. Japanese Unexamined Patent Application Publication No. 2007-297381 discloses a composition having an arteriosclerosis-inhibiting effect containing a bracken extract.

Lythrum anceps ) is a perennial herb that is called perennial herb. It grows up to a height of about 1m in wetlands such as streams and grasses, and the flowers bloom in May-August. Diuretic, branch, and cystitis are known to be effective.

Korean Patent No. 10-0852737 discloses a pharmaceutical composition for prevention or treatment of liver cirrhosis, which comprises an extract of Brassica juncea root having antioxidant and liver protecting effect, in relation to a pharmaceutical or cosmetic composition using Bacillus subtilis. Korean Patent Registration No. 0163119 discloses an oral composition having an effect of preventing and symptomatic relief of tooth decay and periodontal disease, which comprises baconthus or brucia extract. Also, Chinese Patent Publication No. 101637483 discloses a pharmaceutical composition containing flavonoids extracted from Bacillus subtilis, which can be used for anticancer adjuvant therapy.

Walnut trees ( Juglans sinensis ) is a deciduous arboreous tree with a height of about 20m. It grows thick and spreads in all directions. Walnut eggs are known to be effective in eliminating tonic, gangjeong and constipation, and walnut oil is also used to treat skin diseases.

Korean Patent Laid-Open No. 10-2004-0051784 discloses an antioxidant cosmetic composition containing Ghazam, Walnut or Chocophyll extract, and Korean Patent Laid-Open No. 10-2005-0097578 Discloses a cosmetic composition for skin moisturizing comprising a walnut extract. Korean Patent Laid-Open Publication No. 10-2011-0034150 discloses a cosmetic composition for anti-inflammation, irritation and immunity enhancement of skin comprising an extract of a mixture of peach roots, plum roots, plum roots and walnut shells as an active ingredient, Korean Patent Laid-Open No. 10-2009-0018200 discloses an antimicrobial, antifungal and antiviral composition containing a walnut extract.

Painting trees ( Sophora japonica L. ) is a deciduous arboreous tree with flower buds, called fruit. Buds are known to be effective against arteriosclerosis and hypertension, and fruits, branches and bark are known to be effective on hemorrhoids.

Korean Patent Registration No. 10-0312735 discloses an anti-inflammatory analgesic composition that can be used for arthritis, edema, pain and the like containing an extract of Lycopersiconum L. as an active ingredient. -0380865 discloses a pharmaceutical composition for the prevention and treatment of an allergic disease containing an extract of Lycopersicon esculenta as an active ingredient. Korean Patent Registration No. 10-0380865 discloses a pharmaceutical composition for preventing or treating osteoporosis containing an extract of Lactobacillus japonica, and WO2004 / 002435 discloses a composition for slimming containing an extract of Lactobacillus japonica have.

Pine ( Kalopanax pictus ) is a deciduous tree of the Araliaceae and is also called a hornbeam. It is distributed in Korea, Japan, Manchuria, China and so on. The bark is called Haedongpyeong neuralgia, back pain, arthritis, tonsils, fever, kidney disease, diabetes, is known to be effective in restoring fatigue.

Korean Patent Publication No. 2002-0071228 discloses the use of the extract of the mulberry extract which can be used for anticancer, antimicrobial, antioxidant, liver function improvement and hangover resolution in connection with the pharmaceutical or cosmetic composition using the wood, 10-2006-0096130 discloses an antiinflammatory composition containing a sea tangle extract and a ginger extract, and Korean Patent Registration No. 10-0588448 discloses a functional food for improving symptoms of degenerative central nervous system Lt; / RTI > Korean Patent No. 10-0567456 discloses a therapeutic agent for AIDS containing an extract of Panax ginseng as an active ingredient. Korean Patent No. 10-0855314 discloses a pharmaceutical composition for antimicrobial use including a extract of Panax americana and a natural extract, a cosmetic composition And Korean Patent Laid-Open Publication No. 10-1997-0007644 discloses a composition for protecting liver function containing syringin separated from sea tangle, which is the bark of bark. Korean Patent Laid-Open No. 10-2008-0101344 discloses an anti-inflammatory analgesic composition comprising an extract of sea tangle containing tannin as an active ingredient, Korean Patent Laid-Open No. 2003-0033281 discloses a composition comprising Kalopanax saponin A Korean Patent No. 10-0555660 discloses a pharmaceutical composition for treating arthritis comprising an extract of sea tangle as an active ingredient.

The worm ( Orostachys japonica A. Berger ) is a perennial plant belonging to the family Staphylococcus aureus . The leaves are densely run and have no petiole. The flower is white and blooms in September. It is said to be a roofing material that grows well on tile roofs such as old temples and has a liver function recovery, anticancer, and antibacterial effect.

Korean Patent Laid-Open No. 10-2008-0035047 discloses a composition for preventing or treating gastritis and peptic ulcer including watson extract, and Korean Patent Laid-Open No. 10-2010-0115567 Korean Patent No. 10-0966719 discloses a composition for prevention or treatment of obesity and hyperlipidemia comprising an extract of Waksong. Korean Patent Laid-Open No. 10-2008-0020030 discloses a cosmetic composition for preventing skin aging which contains an extract of Wansong, Korean Patent Publication No. 10-2011-0019262 discloses an anti-thrombogenic composition containing an extract of Wansong Korean Patent Registration No. 10-0346583 discloses a cosmetic composition for moisturizing skin containing an extract of Wassong as an active ingredient. Korean Patent Laid-Open Publication No. 10-1011-0042851 discloses a cosmetic composition which is effective for skin moisturizing, soothing and atopy improvement using wax, and Korean Patent Registration No. 10-0614222 discloses a cosmetic composition comprising a warship extract as an active ingredient Korean Patent No. 10-0525815 discloses an antimicrobial composition containing an ethanol extract of watsong, Korean Patent No. 10-0525816 discloses a composition for treating colorectal cancer, which comprises an anti-diabetic ≪ / RTI >

However, there has been no disclosure about the antimicrobial composition in which the extract of the plant or the fraction thereof is irradiated with light.

Accordingly, the present inventors have attempted to develop antibacterial agents, especially antibiotics against methicillin-resistant bacteria, using various plant extracts. As a result, The ethanol extracts or fractions thereof of the wood, goblet, octopus, fennel, fern, bouquet, bracken, fern, walnut, coniferous tree, and apricot have effectively killed the staphylococcus aureus, especially methicillin-resistant Staphylococcus aureus In particular, when the plant extract or fractions thereof are irradiated with light, it has been confirmed that the antimicrobial effect is enhanced, the side effect is small and safe, and the present invention is completed.

It is an object of the present invention to provide a method for producing a plant of the present invention which is composed of a bird bed bark, a paulownia tree, a wormwood, a fig tree, a bamboo, an Indian potato, As an active ingredient, a light irradiation product obtained by irradiating light onto any one or more plant extracts or fractions thereof.

Another object of the present invention is to provide an antimicrobial composition in which the light irradiation product exhibits an antimicrobial effect against bacillus, staphylococcus or staphylococcus.

It is still another object of the present invention to provide the antimicrobial composition for medicines, cleaning agents, cosmetics and the like.

In one embodiment, the present invention relates to a method of making a plant of the present invention, which is made up of a bird bed bark, a paulownia tree, a wormwood, a fig tree, a bamboo, an Indian potato, a woodblock, a goblet, an octagonal fennel, The present invention provides an antimicrobial composition comprising, as an active ingredient, a light irradiation product obtained by irradiating light to any one or more plant extracts or fractions thereof.

In the present invention, the contents of the above-mentioned baby bedbug, paulownia tree, witch hazel, fig tree, chestnut, Indian potato, foxtail, goblet, octopus, foxtail, fern, See above.

Such plants include leaves, flowers, fruits, seeds, stems, roots, husks and outposts of plants, and these plants may be used in combination with one or more of these plants.

In the present invention, the plant extract or the fraction thereof includes not only an extract or a fraction obtained by a solvent of a plant, but also enriched or dried forms of the purified water, the extract, the fraction or the purified water thereof.

The solvent for the plant extract is selected from the group consisting of methanol, ethanol, propanol, isopropanol, butanol, pentanol, hexanol, ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, dichloromethane, N, DMF), dimethylsulfoxide (DMSO), 1,3-butylene glycol, propylene glycol, and water may be used alone or as a mixture of two or more thereof, but are not limited thereto. Preferred are methanol, ethanol, propanol, isopropanol, butanol, pentanol and hexanol.

The solvent for the plant fractions may be selected from the group consisting of methanol, ethanol, propanol, isopropanol, n-butanol, isobutanol, pentanol, hexanol, ethylene, acetone, normal hexane, isohexane, cyclohexane, ether, chloroform, ethyl acetate, But are not limited to, dichloromethane, N, N-dimethylformamide (DMF), dimethylsulfoxide (DMSO), 1,3-butylene glycol, propylene glycol and water alone or as a mixture of two or more thereof . Preferably normal hexanol, ethyl acetate, normal butanol and water.

In one specific implementation, the plant extracts were prepared using ethanol to assess the antimicrobial activity of the plants, and then sequentially fractionated using normal hexane, ethyl acetate, normal butanol and water.

In the present invention, the method for producing the plant extract or its fractions is not particularly limited, and for example, cold extraction, ultrasonic extraction, reflux cooling extraction and the like can be used.

The purified water is a process for removing suspended solid particles from an extract or a fraction. The purified water may be filtered using a cotton, nylon, or the like, or an ultrafiltration method, a freezing filtration method, a centrifugation method, or the like. In addition, it may further comprise a separation process by various chromatographies (size, charge, hydrophobicity or affinity chromatographic).

The extract, fraction or purified product may be used as it is in liquid form or may be used by concentrating and / or drying it. The method of concentration and / or drying includes, but is not limited to, freeze drying, vacuum drying, hot air drying, spray drying, vacuum drying, foam drying, high frequency drying, infrared drying and the like.

In the present invention, the light means light emitted from the sun, a fluorescent lamp, an incandescent lamp, and the like, but is not limited thereto. In addition, the light preferably includes visible light, ultraviolet light, and infrared light.

The light intensity of the light is 2,000 to 18,000 lux, preferably 5,000 to 18,000 lux, more preferably 10,000 to 18,000 lux. Even when the illuminance of the light is less than 2,000 lux, the light irradiated to the plant extract or fraction of the present invention has an antibacterial effect. However, since the irradiation time is prolonged, the effect on energy used is insufficient, In the case of exceeding the lux, it is uneconomical compared to the energy used as compared with that of the illuminance.

On the other hand, the irradiation time of the light is 1 hour or more, preferably 1 to 24 hours, and more preferably 2 to 20 hours. When the irradiation time of the light is less than 1 hour, the antimicrobial effect of the irradiated material on the plant extract or the fraction is not enhanced compared to the case of not irradiated with light, and when the irradiation time exceeds 24 hours, It is insignificant and not economical. However, the irradiation time of the light can be controlled according to the irradiation amount of the light.

In one specific embodiment, the ethanol extracts or fractions thereof were irradiated with 0 to 18,000 lux light to observe the antimicrobial activity against methicillin-susceptible Staphylococcus aureus and methicillin-resistant Staphylococcus aureus, In the case of the ethanol extract of the groundwashing zone and its ethyl acetate fraction, the majority of methicillin-susceptible Staphylococcus aureus and methicillin-resistant Staphylococcus aureus showed a slight antibacterial activity in the case of not irradiating light (0 lux) The antibacterial activity was enhanced. On the other hand, the normal hexane fraction of the ethanol extract of the genus Bombyx mori was superior to the methicillin-susceptible Staphylococcus aureus and methicillin-resistant Staphylococcus aureus in the absence of light (0 lux) The antimicrobial activity was further increased. In addition, the antimicrobial activity was increased as the irradiance of irradiated ethanol extract and fraction thereof were increased, but the antimicrobial activity tended to be decreased at a certain level of illumination. The antimicrobial activity was dependent on the concentration of the fractions.

In another specific embodiment, the ethanol extract of the pine needle leaf or its normal hexane fraction, ethyl acetate fraction, normal butanol fraction and water fraction are irradiated with light of 0 to 18,000 lux to detect methicillin-susceptible Staphylococcus aureus and methicillin resistance As a result of observing the antimicrobial activity against Staphylococcus aureus, the antimicrobial activity of methicillin-susceptible Staphylococcus aureus and methicillin-resistant Staphylococcus aureus did not show or showed slight antimicrobial activity when the light was not irradiated, The antimicrobial activity was enhanced. The antimicrobial activity of the extracts increased with increasing irradiance. However, the antimicrobial activity of the extracts or fractions was dependent on the concentration of the extracts or fractions of P. thunbergii.

In another specific embodiment, the plant is selected from the group consisting of a worm stem, a fig leaf, a lobster, an Indian potato root, a Japanese quince root, a Japanese root, an octagonal fescue, a perianth leaf, a bracken stem, a bracken bloom, , The extracts of the flowers of the tree, the leaves of mandarin, and the green tea were irradiated with light of 4,000 to 18,000 lux to examine the antimicrobial activity against methicillin-susceptible Staphylococcus aureus and methicillin-resistant Staphylococcus aureus. As a result, , The antimicrobial activity was enhanced. The higher the illuminance of the irradiated light, the more the antimicrobial activity was increased. However, the antimicrobial activity tended to be decreased at a certain level of illumination.

Thus, by controlling the concentration of the plant extract or fraction, the intensity of the light irradiated to the extract or fraction, and the irradiation time, it is possible to achieve an equivalent or higher antimicrobial activity while using a light irradiation for a smaller amount of plant extract or fraction Therefore, a safe antimicrobial composition can be used because it is economical and minimizes side effects.

In the present invention, the bacterium having antimicrobial activity against the plant extract or the fractions thereof is not limited to bacterium, staphylococci, and staphylococci, to be. More preferably, the staphylococci include Staphylococcus aureus, Staphylococcus epidermidis, Vancomycin-mediated Staphylococcus aureus and the like, and more preferably, Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pneumoniae, Is a Staphylococcus aureus, and most preferably a methicillin resistant Staphylococcus aureus.

In addition, the composition of the present invention may further include a conventional antimicrobial agent, and the antimicrobial agent of the present invention may include, but not limited to, a penicillin antimicrobial agent, a quinolone antimicrobial agent, a tetracycline antimicrobial agent, a sulfonamide antimicrobial agent , Macrolide series antibacterial agents, and the like, preferably penicillin series antibacterial agents.

The penicillin-based antimicrobial agent has an antibacterial effect by inhibiting bacterial growth by affecting cell wall synthesis of bacteria. Examples thereof include penicillin G, penicillin V, methicillin, oxacillin, cloxacillin, but are not limited to, dicloxacillin, nafcillin, ampicillin, amoxicillin, carbenicillin, ticarcillin, mezlocillin, piperacillin, But is not necessarily limited thereto.

By further including an antimicrobial agent in the antimicrobial composition containing the light extract for the plant extract or the fraction thereof, the antimicrobial activity can be further enhanced due to the antimicrobial activity having different mechanisms, and the antimicrobial activity against the various kinds of bacteria can be further enhanced Lt; / RTI >

The content of the added antimicrobial agent is preferably 0.001 to 30% by weight based on the total composition, but is not limited thereto. If the content of the antibacterial agent is less than 0.001% by weight, the antibacterial effect by the antibacterial agent may be insignificant. If the content is more than 30% by weight, the antibacterial agent may be precipitated or may be uneconomical to the effect.

The antimicrobial composition of the present invention can be used for various purposes for antibacterial purposes, examples of which include medicines, detergents, and cosmetics.

In one specific embodiment, the antimicrobial composition is used as an antimicrobial pharmaceutical composition.

The antimicrobial composition contained in the pharmaceutical composition of the present invention is 0.0001 to 30% by weight, preferably 0.0001 to 10% by weight based on the total weight of the pharmaceutical composition.

The pharmaceutical composition may be formulated into tablets, capsules, powders, granules, suspensions, emulsions, syrups, emulsions, warning agents, ointments, spraying agents , Oil, gel, main tablet, tincture, bath, liniments, lotions, patches, pads, creams and the like. In addition, it can be preferably used as an external preparation for skin intended for topical administration for application directly to the affected area. In this case, a form such as an ointment, a lotion, a spray or a gel is preferable. These external preparations for skin may also be included in a support base or matrix or the like, which can be applied directly to the treatment site, and examples of the support substrate include gauze or bandages. The plant extracts or fractions thereof used in the formulation may be in the form of a colloid or a dried powder.

When formulating with ointment, consideration should be given to skin surface temperature, skin pH, transdermal water loss value, total lipid value of epidermis, etc., and vaseline, liquid paraffin, paraffin, plastic base, silicone, a water-soluble base, an emulsifiable base, a suspension base, and the like, such as vegetable oil, lard, wax, and purified lanolin. Such ointments may be used in combination with antioxidants such as tocopherol, BHA, BHT, NDGA, etc., preservatives such as phenolic substances, chlorobutanol, benzyl alcohol, parabens and benzoic acid, humectants such as glycerin, propylene glycol, sorbitol ), A dissolution aid (e.g., ethanol, propylene glycol), a softening aid (e.g., liquid paraffin, glycerin, propylene glycol, an interfacial surface agent, etc.).

When the composition is formulated into a lotion, it may be prepared as a lotion such as a solution, a suspension or an emulsion. In the case of a lotion applied to the skin, it may be manufactured to have a viscosity of, for example, 200 cps to 500 cps. A wetting agent such as glycerin or propylene glycol is preferably blended.

When formulated as a sprayer, a propellant or the like may be formulated with the additive such that the water-dispersed concentrate or wet powder is dispersed.

When formulated as a patch, an absorption enhancer can be used to increase absorption of the compound through the skin.

The pharmaceutical composition of the present invention may be administered by various administration methods such as oral, parenteral, topical, rectal, intranasal, and the like. In the case of oral administration, for example, oral solid preparations may be formulated with a diluent (e.g., lactose, dextrose, sucrose, cellulose, cornstarch, potato starch, etc.), a lubricant (e.g., silica, talc, , Starch, arabic gum, gelatin methyl cellulose, carboxymethyl cellulose, polyvinyl pyrrolidone, etc.), disintegrating agents (e.g., starch, alginic acid, alginate, Sodium starch glycolate, and the like), formaldehyde, dyes, sweeteners, wetting agents (e.g., lecithin, polysorbate, lauryl sulfate, etc.), and other pharmacologically inert materials commonly used in pharmaceuticals and the like. For topical administration, excipients such as excipients such as lactose, starch, cellulose, lactose, polyethylene glycol, lubricants such as magnesium stearate, stearic acid, glyceryl behenate, talc, Aluminium chloride, etc.) and the like.

Parenteral administration includes injections that result in systemic effects or injections that are administered directly to the affected area. Examples of parenteral administration include subcutaneous, intravenous, intramuscular, intradermal, intrathecal, guiding and general injection techniques.

Topical administration includes the treatment of an affected area or organ that is readily accessible by topical application, such as ear, vaginal, open and sealed wound or closed wound and skin, including eye, ear and middle ear infections. It also includes transdermal delivery, which leads to systemic effects.

Rectal administration includes the form of suppositories.

Intranasal administration includes co aerosol or inhalation application.

The pharmaceutical composition of the present invention is administered in a pharmaceutically effective amount. In the present invention, a pharmaceutically effective amount means an amount sufficient to treat or prevent a disease at a reasonable benefit / risk ratio applicable to medical treatment or prevention. The effective dose level will depend on the type of disease and its severity, Including the age, weight, health and sex of the patient, sensitivity of the patient to the drug, time of administration of the particular extract used, route of administration and rate of release, duration of treatment, Can be determined according to factors well known in the art. Generally, an adult dose of 0.1 to 1000 mg / kg per day, preferably 10 to 100 mg / kg per day, may be administered once to several times per day.

The present invention also relates to a method for treating a bacterium, a bacterium or a spiral bacterium infection, particularly methicillin-resistant Staphylococcus aureus, by administering the above antimicrobial pharmaceutical composition to a subject.

Such treatment refers to therapeutic treatment and prevention or prevention measures. Therefore, those in need of treatment include those who are infected with methicillin-resistant Staphylococcus aureus, as well as those who want to prevent infection with methicillin-resistant Staphylococcus aureus. The term " animal " refers to all animals that are infected with the fungus and require treatment, and specifically include mammals such as humans, cows, dogs, horses and pigs, rodents such as guinea pigs, hamsters, squirrels, and rats, But are not limited to, reptiles such as birds, snakes, lizards, turtles and crocodiles, amphibians such as frogs and salamanders.

In another specific embodiment, the antimicrobial composition is used as an antimicrobial detergent composition.

The detergent refers to materials and products that can build up, remove, or disperse solids and liquid dirt from the surface being cleaned, and the cleaning refers to the removal of unwanted materials or microorganisms from the surface.

The antimicrobial composition contained in the cleaning agent of the present invention is 0.0001 to 30% by weight, preferably 0.0001 to 20% by weight, and most preferably 0.0001 to 10% by weight based on the total weight of the cleaning agent.

The components contained in the cleaning agent of the present invention include, as an active ingredient, components commonly used in detergents in addition to the antimicrobial composition described above. For example, conventional adjuvants such as perfumes, dyes, solvents, opacifying agents, copolymerization system dispersions, thickening agents, cellulose derivatives, krypton gum, albumin derivatives, fats, oils, keratolytic agents, herbicides, thickeners, stabilizers, Carriers and the like.

The detergent of the present invention can be used as a detergent for cleaning the living environment such as a detergent for washing human body such as shampoo, rinse, soap, hand wash, foam cleansing, a dishware, a bathroom, a sink, But is not limited thereto.

The detergent of the present invention can be prepared into any formulation conventionally produced in the art. For example, liquids, solids, creams, and spray agents.

In another specific embodiment, the antimicrobial composition is used as an antimicrobial cosmetic composition.

The antimicrobial composition contained in the cosmetic of the present invention is 0.0001 to 20% by weight, preferably 0.0001 to 10% by weight, and most preferably 0.0001 to 3.0% by weight based on the total weight of the cosmetic.

The ingredients contained in the cosmetic of the present invention include, as an active ingredient, the ingredients commonly used in cosmetics in addition to the antimicrobial composition described above. Such as thickeners, stabilizers, solubilizers, vitamins, customary adjuvants such as pigments and perfumes, and carriers and the like.

The cosmetic composition of the present invention can be prepared into any formulation conventionally produced in the art. For example, they may be formulated as solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations and sprays, But is not limited thereto. More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the cosmetic preparation of the present invention is a paste, a cream or a gel, the carrier component may be an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracer, a cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, calcium silicate Or polyamide powder may be used, and in the case of spray, additionally, propellants such as chlorofluorohydrocarbons, propane / butane or dimethyl ether may be used.

When the cosmetic formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid esters of sorbitan.

When the cosmetic formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the cosmetic preparation of the present invention is a cleansing agent containing an interfacial active agent, it is preferable to use, as the carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, Amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester.

In another aspect, the present invention provides a method for producing an antimicrobial composition comprising the step of producing at least one of a plant extract or fractions thereof, and a step of irradiating light to at least one of the extract or the fraction do.

In the method for producing the antimicrobial composition of the present invention, the first step is a step of producing a plant extract or a fraction thereof. For the contents of the above plant extract or fraction, refer to the above description.

In one specific embodiment, plants are prepared using ethanol and then sequentially fractionated using normal hexane, ethyl acetate, normal butanol and water.

The next step is to irradiate the plant extract or fractions thereof with light. When the plant extract or fractions thereof are irradiated with light, the antimicrobial activity of the plant extract or fractions thereof is enhanced, so that a high antimicrobial activity can be obtained even by using a small amount, which is economical and safe. Refer to the above for the light, the illuminance of the light, and the irradiation time of the light.

The method may further comprise mixing the existing antimicrobial agent in the next step. For the contents of the antimicrobial agent and its contents, refer to the above description.

By further including an antimicrobial agent in the antimicrobial composition containing the plant extract or the fractions thereof, the antimicrobial activity can be further enhanced due to the antimicrobial activity having different mechanisms, and the antimicrobial activity can be exhibited in more various kinds of bacteria.

The above-mentioned plants are natural products which have been used for a long time by folk remedies and have little toxicity and side effects of their own, so they can be used in medicines, detergents and cosmetics without worrying about toxicity and side effects.

Since the antimicrobial composition of the present invention uses various plants which are natural products, there is little concern about side effects, is safe, and has excellent antimicrobial activity, so that it can be usefully used in medicines, cleaning agents or cosmetics. In particular, it has excellent antimicrobial activity against Staphylococcus aureus having resistance to methicillin, so its utility value is very high. Further, since the antimicrobial activity can be enhanced by controlling the intensity of the light irradiated to the antimicrobial composition and the irradiation time, the antimicrobial activity of the antimicrobial composition can be equal to or higher than that of the existing antimicrobial agent using a small amount of the antimicrobial composition, It is low risk, economical and efficient. In addition, since plants that can be easily found in Korea are used, they can be recycled and high value-added products can be sought.

Hereinafter, the present invention will be described in more detail with reference to examples. It is to be understood by those skilled in the art that these embodiments are only for describing the present invention specifically and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention.

Example  1: plant extract and Fraction  Produce

1-1. Collection of plants

It is a kind of plant which has a large number of leaves and leaves. It has a large number of leaves, leaves, leaves, Painted tree branches, mandarin leaves and hanbong flowers were collected from Suncheon in June, 2010. Samples were confirmed by Professor Shin Dong - Won of the Department of Oriental Medicine and Medicine, Sunchon National University.

1-2. Plant Extract and Fraction Production

The plants collected in Example 1-1 were dried and boiled for 3 hours in 1 L of ethanol. Next, the ethanol extract was dissolved in distilled water at a concentration of 10%, and then fractionated in the order of n-hexane, ethyl acetate (EtOAc), normal butanol (n-BuOH) 4 < / RTI >

Example  2: Collection and culture method of strain

2-1. Collection of strains

Clinical isolates (DPS 1-5) for methicillin resistant Staphylococcus aureus (MRSA) were isolated from five patients at Wonkwang University Hospital. The methicillin-resistant strains (methicillin-resistant strain) of S. aureus ATCC 33591 and methicillin-susceptible strains (methicillin-susceptible strain) S. aureus ATCC 25923 was purchased from American Type Culture Collection (ATCC), USA.

2-2. Storage and culture method of strain

The fungi of Example 2-1 were stored in 30% glycerol and then stored frozen at -70 ° C. The fungi were cultured using Mueller-Hinton broth (MHB) and Mueller-Hinton agar (MHA). When cultured in Mueller-Hinton medium, Were suspended in a Mueller-Hinton medium and cultured at 37 DEG C for 24 hours.

Example 3: Arabidopsis land bedbugs Measurement of Antimicrobial Activity of Light Irradiated with Total Ethanol Extract

3-1. Determination of minimum inhibitory concentration

(1) Preparation of sample

Samples were prepared so that the initial ethanol concentration of 2000 mu g / ml of the ethanol extract of the terrestrial broad-leaved panthera.

(2) Determination of minimal inhibitory concentration (MIC)

The minimum inhibitory concentration was determined by the CLSI microdilution broth method. Methicillin-susceptible strains S. aureus ATCC 25923, methicillin resistant strain S. aureus ATCC 33591, and clinical isolates DPS1 to DPS5 for methicillin-resistant Staphylococcus aureus at a concentration of 1.5 x 10 ⁸ CFU / ml. The sample (1) of Experimental Example 3-1 prepared at a concentration of 2000 占 퐂 / ml was diluted twice in 50% DMSO sequentially so that the concentration of the sample was from 2000 占 퐂 / ml to 0.975 占 퐂 / ml . Thereafter, 100 μl of the liquid medium, 10 μl of the bacterial solution and 10 μl of the sample of each concentration were treated in each well of the 96-well plate, and the plate was irradiated under the conditions of not irradiating the light and 2,000, 4,000, 6,000, 8,000, 10,000, 14,000, 16,000 and 18,000 lux of light, and cultured at 37 DEG C for 18 hours. The condition for irradiating light was performed by placing the plate in a plant culture chamber (Vision, Korea) equipped with a fluorescent lamp (Shinkwang Electric L-40D compact fluorescent lamp). The minimum inhibitory concentration was determined by the methicillin susceptibility strain S. aureus ATCC 25923, methicillin-resistant S. aureus ATCC 33591 and methicillin-resistant Staphylococcus aureus strains DPS1 to DPS5 were completely inhibited. The results obtained from the experiments are shown in Table 1 below Respectively.

As a result of the experiment, the ethanol extracts of the outbreaks of the caterpillars showed no antimicrobial activity under the condition of no light irradiation. However, under light irradiation conditions, the antimicrobial activity was observed in both methicillin-sensitive Staphylococcus aureus and methicillin-resistant Staphylococcus aureus, and the antimicrobial activity was increased as the intensity of light increased. However, when the light intensity was above a certain level, the antibacterial activity was decreased. The antimicrobial activity of the irradiated water on the ethanol extracts of the outbreaks of the grasshoppers was found to be maximal when irradiated with light of 10,000 to 16,000 lux depending on the samples.

3-2. Determination of growth inhibition degree

(1) Preparation of strain suspension

In order to measure the antimicrobial activity of the irradiated material on the ethanol extract of the rice seedlings out of the disc diffusion method, S. aureus ATCC 25923, methicillin-resistant strain S. aureus ATCC 33591, and methicillin Clinically isolates DPS1 to DPS5 for resistant Staphylococcus aureus were cultured in Mueller-Hinton agar medium at 37 占 폚 for 24 hours. Thereafter, the strains were inoculated on a Mueller-Hinton medium to prepare a suspension having a turbidity of about 1.5 × 10 ⁸ CFU / ml.

(2) Preparation of sample

Samples were prepared so that the initial ethanol concentration of 2000 mu g / ml of the ethanol extract of the terrestrial broad-leaved panthera.

(3) Inoculation and culture of samples

After the Mueller-Hinton agar broth was poured into a Petri dish, 100 μl of the suspension prepared in (1) of Experimental Example 3-2 was added to each Petri dish, and the suspension was applied. Thereafter, a sterilized paper disk having a diameter of 6 mm was placed in the center of the petri dish. Next, each of the ethanol extracts prepared in (2) of Experimental Example 3-2 was sequentially diluted 2 times with each of the Petri dishes in a Petri dish at a rate of 250 및 and 500 ≪ / RTI > Thereafter, the petri dishes were cultured in a plant growth chamber at 37 ° C for 18 hours under the conditions of not irradiating light and irradiating light at 2,000, 4,000, 6,000, 8,000, 10,000, 12,000, 14,000, 16,000 and 18,000 lux . The light irradiation condition was performed by placing the petri dish in a plant culture chamber (Vision, Korea) equipped with a fluorescent lamp (Shinkwang Electric L-40D compact fluorescent lamp).

(4) Measurement of antimicrobial activity

The antibacterial activity was measured by measuring the diameter at which the growth of the strain was inhibited around each disk after the incubation time of (3) of Example 3-2. The results obtained through the experiment are shown in Table 2 below.

As a result of the experiment, except for the DPS2 group, the ethanol extracts of the rice terraces showed no antimicrobial activity when irradiated with light, but the antimicrobial activity was observed when irradiated with light. And, as the intensity of light increased, the antimicrobial activity was increased. However, when the intensity of light was more than a certain level, the antimicrobial activity was decreased. The antimicrobial activity of the irradiated water on the ethanol extracts of the outbreaks of the grasshoppers was found to be maximal when irradiated with light of 10,000 to 16,000 lux depending on the samples.

In addition, in all the experimental groups, when the strain was loaded at a loading of 500 ug compared to 250 ug of the ethanol extract of the grasshoppers, the growth inhibition diameter of the strain was widely observed and the concentration dependent antimicrobial activity was exhibited.

Example  4: Light on the paulownia leaf ethanol extract Surveyed  Antimicrobial activity measurement

4-1. Determination of minimum inhibitory concentration

(1) Preparation of sample

The ethanol extract of the paulowniae leaves was prepared so that the initial concentration was 2000 μg / ml.

(2) Determination of minimal inhibitory concentration (MIC)

Table 3 shows the results obtained by carrying out the experiment by the method of Example 3-1 to obtain the minimum inhibitory concentration of the irradiated product of the ethanol extract of the paulacialis leaf.

As a result of the experiment, the ethanol extract of Tungus leaves showed no antimicrobial activity except for DPS3, a clinical isolate for methicillin - resistant Staphylococcus aureus, and the antimicrobial activity of DPS3 was very weak. However, under light irradiation conditions, the antimicrobial activity was observed in both methicillin-sensitive Staphylococcus aureus and methicillin-resistant Staphylococcus aureus, and the antimicrobial activity was increased as the intensity of light increased. However, when the intensity of light is above a certain level, the antimicrobial activity is rather reduced. The antimicrobial activity of the irradiated water on the ethanol extract of the paulownia leaves was found to be maximized when light of 12,000 to 18,000 lux was irradiated though it varied depending on the sample.

4-2. Determination of growth inhibition degree

(1) Preparation of strain suspension

In order to measure the antimicrobial activity of the irradiated material on the ethanol extract of paulacialis leaf by the disc diffusion method, the methicillin-sensitive strain S. aureus ATCC 25923, methicillin resistant strain S. aureus ATCC 33591, and clinical isolates DPS1 to DPS5 for methicillin-resistant Staphylococcus aureus were cultured in a Mueller-Hinton agar medium for 18 hours at 37 ° C. Thereafter, the strains were inoculated on a Mueller-Hinton medium to prepare a suspension having a turbidity of about 1.5 × 10 ⁸ CFU / ml.

(2) Preparation of sample

The ethanol extract of the paulowniae leaves was prepared so that the initial concentration was 2000 μg / ml.

(3) Measurement of sample inoculation, culture and antibacterial activity

The results obtained by performing the experiment by the method of Example 3-2 in order to measure the antimicrobial activity of the irradiated water on the ethanol extract of the paulownia tree are shown in Table 4 below.

As a result of the experiment, it was confirmed that the ethanol extract of the paulberry leaves showed no antimicrobial activity in all the groups when the light was not irradiated, but the antimicrobial activity appeared when the light was irradiated. And, as the intensity of light increased, the antimicrobial activity was increased. However, when the intensity of light was more than a certain level, the antimicrobial activity was decreased. The antimicrobial activity of the irradiated water on the ethanol extract of the paulownia leaf was found to be maximal when irradiated with light of 12,000 to 18,000 lux depending on the sample.

Compared with 250 ㎍ of ethanol extract of P. thunbergii in all experimental groups, when the strain was loaded at 500 ㎍, the inhibited diameter of the strain was observed to be broad and the concentration dependent antimicrobial activity was shown.

Example  5: Baby bedbug outpost In the fraction  Light for Korea Surveyed  Antimicrobial activity measurement

5-1. Determination of minimum inhibitory concentration

(1) Preparation of sample

The n-hexane fraction and the ethyl acetate (EtOAc) fraction of the rice bran outpost ethanol extracts were prepared so that the initial concentration was 2000 μg / ml.

(2) Determination of minimal inhibitory concentration (MIC)

In order to determine the minimum inhibitory concentration of the light irradiated to the n-hexane fraction and the ethyl acetate (EtOAc) fraction of the rice terrestrial broadleaf topsoil ethanol extract, the results obtained by the experiment of Example 3-1 were as follows: Table 5 shows the results.

As a result, in the case of the ethyl acetate (EtOAc) fractions of the outbreaks of the rice terraces, the methicillin-susceptible Staphylococcus aureus and the methicillin-resistant Staphylococcus aureus differ in their antibacterial activity Respectively. However, the antimicrobial activity of methicillin - susceptible Staphylococcus aureus and methicillin - resistant Staphylococcus aureus increased under light irradiation conditions, and the antimicrobial activity was increased as the intensity of light increased. However, when the intensity of light is above a certain level, the antimicrobial activity is rather reduced.

However, the fraction of n-hexane in the outbreaks of A. nigella showed excellent antimicrobial activity against methicillin-susceptible Staphylococcus aureus and methicillin-resistant Staphylococcus aureus in the absence of light, The antimicrobial activity was further increased. In addition, the antimicrobial activity was increased as the light intensity increased, but the antimicrobial activity was decreased when the light intensity was above a certain level.

The antimicrobial activity of n-hexane fraction and ethylacetate (EtOAc) fractions of the rice terrestrial larvae outpost was found to be maximized when irradiated with 10,000 to 18,000 lux light depending on the samples.

5-2. Determination of growth inhibition degree

(1) Preparation of strain suspension

In order to measure the antimicrobial activity of light irradiated on the supernatant fraction of rice caterpillars by disc diffusion method, methicillin-susceptible strain S. aureus ATCC 25923, methicillin resistant strain S. aureus ATCC 33591, and clinical isolates DPS1 to DPS5 for methicillin-resistant Staphylococcus aureus were cultured in Mueller-Hinton agar medium for 24 hours at 37 ° C. Thereafter, the strains were inoculated on a Mueller-Hinton medium to prepare a suspension having a turbidity of about 1.5 × 10 ⁸ CFU / ml.

(2) Preparation of sample

The n-hexane fraction and the ethyl acetate (EtOAc) fraction of the rice terrestrial broadleaf seedling ethanol extracts were prepared so as to have an initial concentration of 2000 μg / ml.

(3) Measurement of sample inoculation, culture and antibacterial activity

The results obtained by performing the experiment using the method of Example 3-2 to measure the antimicrobial activity of the light irradiated on the n-hexane fraction and ethyl acetate (EtOAc) Table 6 shows the results.

As a result, the fraction of n-hexane in the outbreaks of A. nigella showed antimicrobial activity against methicillin-susceptible Staphylococcus aureus and methicillin-resistant Staphylococcus aureus in the absence of light, and ethyl acetate (EtOAc) The fractions showed antimicrobial activity against methicillin - resistant Staphylococcus aureus in the absence of light irradiation, though they vary from group to group. In addition, the antimicrobial activity was increased as the intensity of light increased, but the antimicrobial activity was decreased when the intensity of light was more than a certain level.

The antimicrobial activity of n-hexane fraction and ethylacetate (EtOAc) fractions of the rice terrestrial larvae outpost was found to be maximized when irradiated with 10,000 to 18,000 lux light depending on the samples.

In addition, in all the experimental groups, when the strain was loaded at a load of 500 μg compared to 250 μg of the fraction of the outbreaks, the inhibition of the growth of the strain was observed to have a concentration-dependent antimicrobial activity.

Example  6: Paulownia leaves In the fraction  Light for Korea Surveyed  Antimicrobial activity measurement

6-1. Determination of minimum inhibitory concentration

(1) Preparation of sample

The n-hexane fraction, ethyl acetate (EtOAc) fraction, n-BuOH fraction, water fraction, ampicillin and oxacillin of the paulowniae leaf ethanol extract were dissolved in 2000 Mu] g / ml. Ampicillin and oxacillin were used as positive control.

(2) Determination of minimal inhibitory concentration (MIC)

The minimum amount of light irradiated on the n-hexane fraction, ethyl acetate (EtOAc) fraction, n-BuOH fraction, water fraction, ampicillin and oxacillin of the paulownia leaf ethanol extract The results obtained by performing the experiment by the method of Example 3-1 in order to obtain the inhibition concentration are shown in Table 7 below.

As a result, in the case of the normal hexane fraction, ethyl acetate (EtOAc) fraction, normal butanol (n-BuOH) fraction and oxacillin of the paulownia leaves, methicillin-susceptible Staphylococcus aureus Methicillin-resistant Staphylococcus aureus showed a slight antimicrobial activity although there was a difference depending on the samples. In the case of ampicillin, the methicillin-resistant Staphylococcus aureus showed a weak antibacterial activity under the condition not irradiated with light, but the antimicrobial activity was excellent in methicillin-susceptible Staphylococcus aureus. However, the antimicrobial activity of methicillin - susceptible Staphylococcus aureus and methicillin - resistant Staphylococcus aureus increased under light irradiation conditions, and the antimicrobial activity was increased as the intensity of light increased. However, when the intensity of light is above a certain level, the antimicrobial activity is rather reduced.

The antimicrobial activity of the light irradiated on the normal hexane fraction, ethyl acetate (EtOAc) fraction, normal butanol (n-BuOH) fraction and water fraction of the paulownia leaf varies depending on the sample, but light of 12,000 to 18,000 lux When it is surveyed, it becomes maximum.

6-2. Determination of growth inhibition degree

(1) Preparation of strain suspension

In order to measure the antimicrobial activity of light irradiated to leaf fractions of paulowniae by disc diffusion method, methicillin-susceptible S. aureus ATCC 25923, methicillin resistant strain S. aureus ATCC 33591, and clinical isolates DPS1 and DPS2 for methicillin resistant Staphylococcus aureus were cultured in a Mueller-Hinton agar medium for 24 hours at 37 ° C. Thereafter, the strains were inoculated on a Mueller-Hinton medium to prepare a suspension having a turbidity of about 1.5 × 10 ⁸ CFU / ml.

(2) Preparation of sample

The n-hexane fraction, the ethyl acetate (EtOAc) fraction, the n-BuOH fraction, the water fraction, the ampicillin and the oxacillin of the ethanol extract of Paulownia leaf / Ml, and ampicillin and oxacillin were used as positive control.

(3) Measurement of sample inoculation, culture and antibacterial activity

Antimicrobial activity of light irradiated on normal hexane fraction, ethyl acetate (EtOAc) fraction, n-BuOH fraction, water fraction, ampicillin and oxacillin of ethanol extract of paulberry leaf The results obtained by performing the experiment using the method of Example 3-2 to measure the activity are shown in Table 8 below.

As a result of the experiment, in the case of n-hexane fraction, ethyl acetate (EtOAc) fraction, normal butanol (n-BuOH) fraction and water fraction of paulownia leaf, methicillin-susceptible Staphylococcus aureus But did not show antimicrobial activity in all of the resistant S. aureus strains. However, when the light was irradiated, the antimicrobial activity was exhibited and the antimicrobial activity was increased as the intensity of light was increased. However, when the intensity of light is above a certain level, the antimicrobial activity is rather reduced.

The antimicrobial activity of the light irradiated on the normal hexane fraction, ethyl acetate (EtOAc) fraction, normal butanol fraction (n-BuOH) and water fraction of the paulownia leaves varies depending on the sample, but light of 10,000 to 16,000 lux When it is surveyed, it becomes maximum.

In addition, in all the experimental groups, the inhibition of strain growth was observed to be larger than that of 250 μg of the fraction of the tungus leaf, and the concentration dependent antimicrobial activity was observed.

Ampicillin, a positive control, showed antimicrobial activity in all tested groups regardless of whether light was irradiated or not. The antimicrobial activity tended to increase with increasing light intensity, but the difference was not significant. Light intensity The antimicrobial activity was decreased. However, oxacillin showed no antimicrobial activity except for the DPS2 group.

Example  7: Light for other plant extracts Surveyed  Antimicrobial activity measurement

7-1. Preparation of samples

And the leaves of the leaves and the leaves of the leaves of the walnut, the leaves of the leaves of the fig tree, the leaves of the Indian potatoes, the leaves of the Japanese oak, the leaves of the Japanese oak, the leaves of the Japanese apricot, the leaves of the octopus, The ethanol extracts of each of the wansong flowers were prepared to have an initial concentration of 2000 μg / ml.

7-2. Determination of minimum inhibitory concentration

And the leaves of the leaves and the leaves of the leaves of the walnut, the leaves of the leaves of the fig tree, the leaves of the Indian potatoes, the leaves of the Japanese oak, the leaves of the Japanese oak, the leaves of the Japanese apricot, the leaves of the octopus, The results obtained by carrying out the experiment by the method of Example 3-1 in order to obtain the minimum inhibitory concentration of the irradiated product of the ethanol extract of each of the flowers of Watermelon are shown in Table 9 below.

As a result of the experiment, it was found that the seeds of the leaves and leaves of the leaves, the leaves of the fig trees, the leaves of the Indian potatoes, the leaves of the Japanese potatoes, the leaves of the Japanese apricot, The antimicrobial activity of methicillin-susceptible Staphylococcus aureus and methicillin-resistant Staphylococcus aureus was not observed in all of the ethanol extracts of fruit husks, tree branches, mandarin leaves and salted flowers. However, under light irradiation conditions, the antimicrobial activity was observed in both methicillin-sensitive Staphylococcus aureus and methicillin-resistant Staphylococcus aureus, and the antimicrobial activity was increased as the intensity of light increased. However, when the intensity of light is above a certain level, the antimicrobial activity is rather reduced. The antimicrobial activity of the irradiated material on ethanol extracts of the above plants was found to be maximized when light of 12,000 to 18,000 lux was irradiated though it depends on kinds of plants and kinds of bacteria.

Example  8: Acute Oral Toxicity Test

In order to confirm that the plant extract of the present invention is not toxic, an acute oral toxicity test was conducted. Twenty SPF SD rats aged 5 to 6 weeks as an experimental animal were used for an acute toxicity test under the following conditions.

Temperature and Humidity Conditions: Temperature 22 degrees +/- 2 degrees, relative humidity 50 +/- 10%

Contrast Cycle: Fluorescent lighting (Turns off at 8 o'clock then turns off at 20 o'clock)

Illumination: 200 to 300 lux

UV-treated drinking water is free.

The test substance was diluted in sterilized distilled water to prepare a test sample. The same amount of sterilized distilled water was used as a control.

On the day of administration, the general condition was observed every hour up to 4 hours, and one day from the next day until the 14th day, careful observation of changes in general condition, poisoning symptoms, motility, appearance, autonomic nerves and dead animals, The pathological anatomical examination was performed to determine the presence or absence of toxicity. As a result, the LD ₅₀ was 3000 mg / kg BW and no toxicity was observed.

Claims (5)

A visible light of 2,000 lux to 18,000 lux is applied to the leaf extract of fig tree for 1 to 24 hours or a fraction of leaf extract of Fig tree is irradiated with visible light of 2,000 lux to 18,000 lux for 1 to 24 hours to be effective ≪ / RTI > as an active ingredient.
A visible light of 2,000 lux to 18,000 lux is applied to the leaf extract of fig tree for 1 to 24 hours or a fraction of leaf extract of Fig tree is irradiated with visible light of 2,000 lux to 18,000 lux for 1 to 24 hours to be effective Wherein the cleaning agent composition is an antistaphylococcal composition.
The leaf extract of fig tree is irradiated with a visible light of 2,000 lux to 18,000 lux for 1 to 24 hours or a fraction of leaf extract of fig tree is irradiated with visible light of 2,000 lux to 18,000 lux for 1 to 24 hours, A cosmetic composition comprising a light irradiation product having an antibacterial effect against a bacterium as an active ingredient. delete delete