KR102540960B1 - Antibacterial composition comprising fig enzyme - Google Patents

Abstract

The present invention relates to an antibacterial composition comprising a mushroom extract containing fig enzymes, a zinc mixture and beta-glucan. The mushroom extract is prepared from plant leaf extracts and can have skin condition improving effects such as skin moisturizing and skin irritation alleviation. In addition, fig enzymes can have excellent antibacterial and antioxidant effects by irradiating visible light, thereby being applied to functional cosmetics.

Description

Antibacterial composition comprising fig enzyme {Antibacterial composition comprising fig enzyme}

The present invention relates to an antibacterial composition comprising a mushroom extract containing fig enzyme, a zinc mixture and beta-glucan, wherein the mushroom extract is prepared from a plant leaf extract and can have skin improving effects such as moisturizing the skin and relieving skin irritation . In addition, by irradiating visible light on the fig enzyme, it can have excellent antibacterial and antioxidant effects, and can be applied to functional cosmetics.

Cosmetics are a representative consumer product, and as interest in the appearance of men as well as women increases, the demand for cosmetics is gradually expanding. Accordingly, various men's cosmetics ranging from basic cosmetics for skin care to color cosmetics are being developed, and recently, male cleansers for washing the genital area or armpits, such as female cleansers, have been actively developed.

On the other hand, in accordance with the recent pursuit of naturalism, the types of cosmetic materials are diversifying, ranging from vegetable raw materials including existing herbal medicines to organic, native plants, rare plants, marine organisms, microorganisms, and animal and plant stem cells, and materials made using biotechnology The proportion of cosmetics containing as an ingredient is steadily increasing.

Functions are also diversifying according to the raw materials of cosmetics. For example, natural substances such as iris, aloe, and seaweed help prevent wrinkles and regenerate cells by promoting skin metabolism and activating cells. , green tea extract has an antioxidant function, and moth bark extract has a whitening function.

Along with the development of functional natural materials, as the stability and efficacy of cosmetic raw materials are emphasized, material discovery technology and processing technology to increase the functionality of materials (microbial culture technology, genetic recombination technology, fermentation technology, stem cell technology, protein technology, etc.) ), verification of functionality to maximize the function of the material, and research on formulation (formulation) technology are being actively conducted.

On the other hand, figs ( Ficus carica ) have been reported in several studies that the fruit flesh, leaves, and sap extracts of figs have antibacterial and antifungal effects, Khaleel Ibrahim Rashid, 2014. Amirah Nadiah Ali, 2018., Shahzada Nadeem Abbas, 2019. According to reports by et al., it has been confirmed that fig extracts exhibit strong antibacterial and antifungal effects against various bacteria and fungi.

As disclosed in Korean Patent Registration No. 10-2450505, the present inventor developed a male cleanser based on the excellent antibacterial and antifungal effects of a mixture of fig enzyme and zinc, and minimized skin irritation based on natural extracts to prove safety, It has been demonstrated that genital hygiene can be improved by reducing odor.

Accordingly, in the present invention, the antibacterial and antifungal effect of figs was paid attention to, and the previously identified technology was applied and expanded. We aim to identify raw materials that can maximize safety, formulation stability, and functional efficacy on human skin, and develop new natural cosmetics.

Korean Patent Registration No. 10-2450505

An object of the present invention is to provide an antibacterial composition comprising a fig enzyme, a zinc mixture and a mushroom extract.

Another object of the present invention is to provide an antibacterial composition containing fig enzyme, zinc mixture and beta-glucan (β-glucan).

Another object of the present invention is to provide a cosmetic composition for moisturizing or antioxidant skin containing a fig enzyme, a zinc mixture and a mushroom extract.

One aspect of the present invention relates to an antibacterial composition comprising 0.1 to 3 parts by weight of fig enzyme extract, 0.05 to 0.5 parts by weight of zinc pyrithione, 0.5 to 5 parts by weight of sodium polynaphthalenesulfonate and 0.1 to 2 parts by weight of mushroom extract. As such, the mushroom extract relates to an antibacterial composition obtained by extracting any one or more plant leaf extracts selected from the group consisting of echinacea leaves, hydrangea leaves and green tea leaves as a solvent.

In the present invention, an antibacterial agent means a substance that has an antibacterial action against bacteria, specifically, a substance that acts as an antibacterial agent by inhibiting a system in which bacteria synthesize a cell wall or protein, or a substance prepared from such a substance.

Representative among various bacteria resistant to the antimicrobial agent are methicillin resistant Staphylococcus aureus (MRSA), vancomycin resistant enterococci (VRE), and vancomycin intermediate Staphylococcus aureus (VISA). ), and Escherichia coli ( E.coli ), which is resistant to at least six or more antimicrobial agents.

Specifically, the composition may be a composition having antibacterial efficacy against at least one selected from the group consisting of Candida albicans , Staphylococcus aureus and Escherichia coli .

In the present invention, the fig tree ( Ficus carcica L ) is a deciduous shrub belonging to the Moraceae family and is native to the Mediterranean coast of western Asia. The tree is 2-4m high, the bark is grayish white, the leaves are alternate, wide egg-shaped, thick, the surface is rough, and the back side has hairs. The latex is known to be effective for athlete's foot, swelling, hemorrhoids, the leaves for athlete's foot or neuralgia, and the fruit for lowering blood pressure and constipation.

On the other hand, it has been reported in several studies that the fruit flesh, leaf, and sap extract of the fig have antibacterial and antifungal effects. Reports by Khaleel Ibrahim Rashid, 2014., Amirah Nadiah Ali, 2018., and Shahzada Nadeem Abbas, 2019. disclose that fig extracts have strong antibacterial and antifungal effects against various bacteria and fungi. Accordingly, in the present invention, it is intended to provide an antibacterial / cosmetic composition that can be used even for sensitive skin while enhancing the antibacterial effect of the fig enzyme and having a skin improvement effect.

The fig enzyme extract of the present invention can be obtained by extracting fig fruits with hot water and an organic solvent. Specifically, after harvesting and pulverizing fig fruits, 1 to 50 times the amount of solvent may be added and extracted at room temperature for 12 hours to 3 months by immersion, compression, or pulverization. The extraction solvent may be fractionated and extracted using at least one solvent selected from the group consisting of purified water, lower alcohol having 1 to 4 carbon atoms, dichloromethane, chloroform, and ethyl acetate.

The mushroom may be any one or more selected from the group consisting of cauliflower mushroom, chaga mushroom, Ganoderma lucidum mushroom, and Sanghwang mushroom, but the type of mushroom containing beta-glucan is not limited.

In the present invention, "β-glucan" is a kind of polysaccharide, and is contained in a large amount in yeast cell walls, mushrooms, grains, etc. to help enhance immunity, and activates the immune function of human normal cells to promote cancer cell proliferation and It is known to suppress recurrence, reduce blood sugar and blood cholesterol, and improve lipid metabolism to suppress the formation and accumulation of body fat.

Among the mushrooms containing a large amount of beta-glucan, the most widely known flower mushroom ( Sparassis crispa ) is a basidiomycete that grows wild on the edges of cut stumps or dead trees of conifers from summer to autumn in Korea, Japan, Europe, North America and Australia. It is a mushroom belonging to the mushroom family, and the fruiting body is 10 to 25 cm high, fleshy, and the lower part is a common stalk with a thick stem. The layer is developed on the back side of the petals, has a good chew and smells like a matsutake mushroom, and is called a flower mushroom because it has a flower shape.

In the present invention, a mushroom extract containing beta-glucan can be obtained by mixing and extracting at least one plant leaf extract selected from the group consisting of echinacea leaves, hydrangea leaves, and green tea leaves with alcohol in a certain ratio.

The Echinacea ( Echinacea Angustifolia ) is a wild plant of the Asteraceae family that lives in the Great Plains of North America and has been widely used as a traditional plant medicine for enhancing immune function against diseases caused by colds or other viral infections for centuries. Recently, Echinacea The extract has been applied as a cosmetic raw material for skin protection. The Echinacea extract has antioxidant, immune enhancement, reactive oxygen species scavenging, and melanin inhibitory effects.

The hydrangea ( Hydrangea macrophylla ) is a tree distributed in the alpine regions of Korea, China, and Japan, and is known to have effects such as fat and cholesterol reduction and antioxidant action by containing saponin. In addition to this effect, a new functionality that can improve muscle function has been reported.

The zinc pyrithione is a substance having a chemical formula of C ₁₀ H ₁₀ N ₂ O ₂ S ₂ Zn (CAS No. 13463-41-7) and is used as a preservative because of its antibacterial and antifungal properties. ( Malassezia ) Due to its effect of inhibiting the proliferation of dandruff and hair loss treatment has been used as a component of hair wash.

The zinc pyrithione has a solubility in water as low as 15 ppm, and may show a suspension phenomenon in cosmetics or external preparations. In order to alleviate this suspension, the antimicrobial composition of the present invention may further include 0.1 to 5 parts by weight of sodium polynaphthalenesulfonate and/or cellulose gum as an emulsion stabilizer based on the total weight to form a zinc pyrithione complex. More specifically, it may include 0.05 to 0.5 parts by weight of zinc pyrithione and 0.5 to 5 parts by weight of sodium polynaphthalenesulfonate.

Another aspect of the present invention includes 0.1 to 3 parts by weight of fig enzyme extract, 0.05 to 0.5 parts by weight of zinc pyrithione, 0.5 to 5 parts by weight of sodium polynaphthalenesulfonate and 0.1 to 3 parts by weight of beta-glucan (β-glucan) It relates to an antibacterial composition, wherein the beta-glucan is derived from a mushroom extract prepared by using at least one plant leaf extract selected from the group consisting of echinacea leaves, hydrangea leaves, and green tea leaves as a solvent.

The composition may further include saw palmetto extract.

On the other hand, it has recently been known that when light is used for natural extracts, the antibacterial activity significantly increases even when a low concentration is applied (Araujo NC, Fontana CR, Bagnato VS, et al. Photodynamic effects of curcumin against cariogenic pathogens. Photomed Laser Surg 2012 ;30(7):393-399). For example, when a 405nm light source is applied to Zanthoriza extract, the minimum inhibitory concentration for S.mutansbiofilm is further lowered, and research results have been reported that a stronger antimicrobial effect is shown when mixed with curcumin (Hwang Hye-rim, et al. Against Streptococcus mutans) Photodynamic antibacterial effect of ginger and natural extracts (2019).

In the present invention, as a result of treating the light source to increase the antimicrobial activity of the fig enzyme extract and mushroom extract, it was found that there is an excellent antibacterial effect against the causative agent of bacterial infection in the human body. Visible light may be irradiated for 1 minute to 60 minutes.

The antibacterial composition of the present invention may be used for various purposes for antibacterial purposes, examples thereof include pharmaceuticals, cosmetics, and the like.

The antibacterial composition may be used as an antibacterial pharmaceutical composition.

Specifically, the antimicrobial composition included in the pharmaceutical composition is 0.0001 to 30% by weight, preferably 0.0001 to 10% by weight based on the total weight of the pharmaceutical composition.

The pharmaceutical composition may be formulated in conventional formulations in the pharmaceutical field according to the purpose of treatment, for example, tablets, capsules, powders, granules, suspensions, emulsions, syrups, emulsions, warnings, ointments, sprays , Oil agent, gel agent, spirit agent, tincture agent, bath liniment agent, lotion agent, patch agent, pad agent, cream agent, etc. can be formulated. In addition, it can be preferably used as an external skin preparation for the purpose of topical administration applied directly to the affected area. In this case, the form of an ointment, lotion, spray, gel, etc. is preferable. These external skin preparations may also be included in a support base or matrix that can be applied directly to the treatment site, and examples of the support base include gauze or a bandage. Plant extracts or fractions thereof used in the formulation may be in colloidal form or dried powder form.

When formulated as an ointment, skin surface temperature, skin pH, transdermal water loss value, total lipid level of the epidermis, etc. should be considered, and vaseline, liquid paraffin, paraffin, plastic base, silicone, lard (lard), vegetable oil, wax, purified lanolin (purified lanolin), such as oil-based substrates, water-soluble substrates, emulsion-based substrates, suspending substrates, etc. to formulate. These ointments contain antioxidants (e.g., tocopherol, BHA, BHT, NDGA, etc.), preservatives (e.g., phenolic substances, chlorobutanol, benzyl alcohol, parabens, benzoic acid, etc.), humectants (e.g., glycerin, propyleneglycone, sorbitol). ), solubilization aids (eg, ethanol, propylene glycol), softening aids (eg, liquid paraffin, glycerin, propylene glycol, surfactants, etc.) may be mixed with additives.

When formulated as a lotion, it may be prepared as a solution type, suspension type or emulsion type lotion, and in the case of a lotion applied to the skin, for example, it may be prepared to have a viscosity of 200cps to 500cps, and feels soft when applied. It is preferable to prepare by blending a wetting agent such as glycerin or propylene glycol in order to give a.

When formulated as a spray, a propellant or the like may be mixed with additives so that the water-dispersed concentrate or wet powder is dispersed.

When formulated as a patch, an absorption enhancer may be used to increase the absorption of the compound through the skin.

The pharmaceutical composition of the present invention can be administered by various administration methods such as oral, parenteral, topical, rectal, and intranasal administration. For oral administration, for example, solid oral dosage forms may contain active ingredients together with diluents (e.g., lactose, dextrose, sucrose, cellulose, corn starch, potato starch, etc.), glidants (e.g., silica, talc, stearic acid). , magnesium or calcium stearate, polyethylene glycol, etc.), binders (e.g. starch, arabic gum, gelatin methylcellulose, carboxymethylcellulose, polyvinyl pyrrolidone, etc.), disintegrants (e.g. starch, alginic acid, alginates, sodium starch glycolate, etc.), formal mixtures, dyes, sweeteners, wetting agents (eg, lecithin, polysorbate, lauryl sulfate, etc.), other pharmacologically inactive substances commonly used in pharmaceuticals, and the like. For topical administration, excipients (e.g., lactose, starch, cellulose, milk sugar, polyethylene glycol, etc.), lubricants (e.g., magnesium stearate, stearic acid, glyceryl behenate, talc, etc.), preservatives (e.g., benzylalkonium chloride) etc.) and the like.

Parenteral administration includes injections that produce a systemic effect or are administered directly to the affected area. Examples of parenteral administration include subcutaneous, intravenous, intramuscular, intradermal, intrathecal, intraocular and general infusion techniques.

Topical administration includes the treatment of easily accessible infected areas or organs by topical application, such as, for example, the ear, vagina, open and closed wounds and skin, including infections of the eye, outer ear and middle ear. Also included is transdermal delivery, which results in a systemic effect.

The pharmaceutical composition of the present invention is administered in a pharmaceutically effective amount. In the present invention, a pharmacologically effective amount means an amount sufficient to treat or prevent a disease with a reasonable benefit/risk ratio applicable to medical treatment or prevention, and the effective dose level is the type and severity of the disease, the activity of the drug, the patient Age, weight, health and sex of the patient, sensitivity to the drug of the patient, time of administration of the specific extract used, route of administration and excretion rate, duration of treatment, factors including drugs used in combination or concurrently with the specific extract used, and other medical factors. It may be determined according to factors well known in the art. Generally, an amount of 0.1 to 1000 mg/kg per day to an adult, preferably a dose of 10 to 100 mg/kg, can be administered once to several times a day.

In addition, the present invention relates to a method for treating bacillus, cocci or spiral bacillus infections, particularly methicillin-resistant Staphylococcus aureus, by administering the antibacterial pharmaceutical composition to a subject.

The treatment refers to therapeutic treatment and prophylactic or prevention measures. Therefore, those in need of treatment include those infected with methicillin-resistant Staphylococcus aureus as well as those who wish to prevent infection with methicillin-resistant Staphylococcus aureus. In addition, the subject refers to all animals infected with the fungus and requiring treatment, specifically, mammals such as humans, cows, dogs, horses, and pigs, rodents such as guinea pigs, hamsters, squirrels, rats, chickens, pheasants, sparrows, etc. Reptiles such as birds, snakes, lizards, turtles and crocodiles, amphibians such as frogs and salamanders, but are not limited thereto.

The antibacterial composition may be used as an antibacterial cosmetic composition.

Another aspect of the present invention is fig enzyme extract 0.1 to 3 parts by weight, zinc pyrithione 0.05 to 0.5 parts by weight, sodium polynaphthalenesulfonate 0.5 to 5 parts by weight, mushroom extract 0.1 to 2 parts by weight and saw palmetto extract A cosmetic composition for skin moisturizing or antioxidant comprising 1-8 parts by weight of lecithin, 1-5 parts by weight of caprylyl glycol, 1-5 parts by weight of caprylic/capric triglyceride, 3 to 10 parts by weight of glycerin are mixed and emulsified under high pressure to contain liposomes carrying active ingredients, and the mushroom extract is selected from the group consisting of echinacea leaves, hydrangea leaves, and green tea leaves. , It relates to a cosmetic composition.

Liposomes of the cosmetic composition generally surround a polar substance or polymeric substance that is difficult to penetrate the cell membrane of the skin with a lipid membrane of a component similar to a biological membrane to help permeate the cell membrane and promote absorption of active ingredients into the body.

Specifically, the cosmetic composition included in the cosmetics is 0.0001 to 20% by weight, preferably 0.0001 to 10% by weight, and most preferably 0.0001 to 3.0% by weight based on the total weight of the cosmetic.

The cosmetic composition may be a male cleanser.

The cosmetic composition may further include skin irritation and erythema relief.

Ingredients included in the cosmetic of the present invention include components commonly used in cosmetics in addition to the antimicrobial composition as an active ingredient. and conventional adjuvants and carriers such as, for example, thickeners, stabilizers, solubilizers, vitamins, pigments and flavors.

The cosmetic composition of the present invention can be prepared in any formulation conventionally prepared in the art. For example, it may be formulated as a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray, etc. It is not limited. More specifically, it may be prepared in the form of softening lotion, nourishing lotion, nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the cosmetic formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, calcium silicate as a carrier component A gate or polyamide powder may be used, and a propellant such as chlorofluorohydrocarbon, propane/butane or dimethyl ether may be additionally included, particularly in the case of a spray.

When the cosmetic formulation of the present invention is a solution or emulsion, a solvent, solubilizing agent, or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol , 1,3-butyl glycol oil, fatty acid esters of glycerol, polyethylene glycol or sorbitan.

When the cosmetic formulation of the present invention is a suspension, liquid diluents such as water, ethanol or propylene glycol, ethoxylated isostearyl alcohol, suspending agents such as polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracanth and the like may be used.

When the cosmetic formulation of the present invention is surfactant-containing cleansing, as carrier components, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid Amide ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

The present invention can provide an antibacterial composition with significantly improved antibacterial and antifungal effects by including beta-glucan or a mushroom extract containing the same, and can minimize skin irritation by utilizing natural extracts.

In addition, by irradiating the natural extract with visible light for a certain period of time, it is possible to have a skin improvement effect by increasing the solubility of natural materials as well as enhancing antibacterial and antioxidant effects.

The effects of the present invention are not limited to the above effects, and should be understood to include all effects that can be inferred from the configuration of the invention described in the detailed description or claims of the present invention.

Figure 1 shows the results of the cytotoxicity test of the antibacterial composition.
Figure 2 shows the results of confirming the antifungal effect of the antibacterial composition on Candida albicans .
Figure 3 shows the results of measuring the amount of hyaluronic acid produced by the antibacterial composition.

Hereinafter, the embodiments of the present invention will be described in detail with reference to the accompanying drawings, but it is obvious that the present invention is not limited by the following examples.

Preparation Example 1. Fig enzyme extract

1-1. Preparation of fig enzyme extract

After collecting and pulverizing fig fruits of the Bongnaesi variety cultivated in Yeongam-gun, Jeollanam-do, 10 times the weight of an ethanol solvent was added and extracted by immersion at room temperature for 24 hours, and suction filtered with a Buchner funnel. By mixing the ethanol was removed using a vacuum concentrator at 50 ℃ to obtain a fig enzyme extract. The extraction and concentration process was repeated three times.

1-2. Preparation of fig enzyme fraction

The fig enzyme extract obtained in Preparation Example 1-1 was extracted three times with a 5-fold weight n-hexane (hexane) solvent and then concentrated under reduced pressure to obtain a fig enzyme hexane fraction. Subsequently, the same extraction was performed with chloroform, ethyl acetate, and n-butanol solvents to obtain the fig enzyme chloroform fraction (Preparation Example 1-2) and the fig enzyme ethyl acetate fraction (Preparation Example 1-3) and fig enzyme butanol fraction (Preparation Example 1-4), respectively.

Preparation Example 2. Preparation of mushroom extract and beta-glucan isolated therefrom

2-1. Preparation of mushroom extract using echinacea leaf extract

The purchased Echinacea leaves were dried and pulverized to obtain dried Echinacea leaf powder. After adding 20 times the weight of water to the obtained dried Echinacea powder, it was boiled for 4 hours to perform hot water extraction, followed by filtration and lyophilization of the filtrate to obtain an Echinacea leaf hot water extract.

Then, a mushroom extract was prepared using the Echinacea leaf extract as a solvent. Specifically, 3L of a mixed solvent mixed with alcohol (fermented alcohol, Daehan Alcohol Life Co., Ltd.) and the Echinacea leaf extract at a ratio of 8: 2 was added to 500 g of dried and crushed flower mushrooms, and then immersed at room temperature. After allowing to stand for 72 hours, use a broth pack (medium, 25 cm X 35 cm, TNC Electronics Co., Ltd.) to filter particles of 100 μm or less through, and then use a vacuum concentrator to remove the solvent. A mushroom extract was prepared by removing the solvent for a period of time.

2-2. Preparation of mushroom extract using hydrangea leaf extract

The purchased hydrangea leaves were dried and pulverized to obtain dry hydrangea leaf powder. The obtained dry powder of hydrangea leaves was added with 20 times the weight of water, boiled for 4 hours, hot water extracted from hydrangea leaves, filtered, and the filtrate was freeze-dried to obtain a hot water extract.

Then, a mushroom extract was prepared using the hydrangea leaf extract as a solvent. Specifically, 3L of a mixed solvent in which alcohol (fermented alcohol, Daehan Alcohol Life Co., Ltd.) and the hydrangea leaf extract were mixed at a ratio of 8: 2 was added to 500 g of dried and crushed flower mushrooms, and then immersed at 72 g at room temperature. After letting it stand for a while, use a soup pack (medium, 25cm X 35cm, TNC Electronics Co., Ltd.) to filter particles of 100um or less, and then use a vacuum concentrator to remove the solvent for 6 hours at 50 ° C. During the removal of the solvent to prepare a mushroom extract.

2-3. Preparation of mushroom extract using green tea leaf extract

The purchased green tea leaves were dried and pulverized to obtain dried green tea leaf powder. After adding 20 times the weight of water to the obtained green tea leaf extract, it was boiled for 4 hours, extracted with hot water from green tea leaves, filtered, and the filtrate was freeze-dried to obtain a hot water extract.

Then, a mushroom extract was prepared using the green tea leaf extract as a solvent. Specifically, 3L of a mixed solvent in which alcohol (fermented alcohol, Daehan Alcohol Life Co., Ltd.) and the green tea leaf extract were mixed in a ratio of 8: 2 was added to 500 g of dried and crushed flower mushrooms, and then immersed in 72 After letting it stand for a while, use a soup pack (medium, 25cm X 35cm, TNC Electronics Co., Ltd.) to filter particles of 100um or less, and then use a vacuum concentrator to remove the solvent for 6 hours at 50 ° C. During the removal of the solvent to prepare a mushroom extract.

2-4. Preparation of beta-glucan derived from mushroom extract using echinacea leaf extract

0.6 to 0.7% (v/v) of Viscozyme was added to the mushroom extract of Preparation Example 2-1, and the product obtained by stirring at a temperature of 40 to 60 ° C. for 6 to 8 hours was pH adjusted using 0.01 N NaOH. 7, followed by hot water extraction at 80-90 ° C. for 24 hours, followed by purification by adding ethanol 2-4 times, and concentration and freeze-drying to obtain beta-glucan.

2-5. Preparation of mushroom extract-derived beta-glucan using hydrangea leaf extract

0.6 to 0.7% (v/v) of Viscozyme was added to the mushroom extract of Preparation Example 2-2, and the product obtained by stirring at a temperature of 40 to 60 ° C. for 6 to 8 hours was pH adjusted using 0.01N NaOH. 7, followed by hot water extraction at 80-90 ° C. for 24 hours, followed by purification by adding ethanol 2-4 times, and concentration and freeze-drying to obtain beta-glucan.

2-6. Preparation of mushroom extract-derived beta-glucan using green tea leaf extract

0.6 to 0.7% (v / v) of Viscozyme was added to the mushroom extract of Preparation Example 2-3, and the product obtained by stirring at a temperature of 40 to 60 ° C. for 6 to 8 hours was pH adjusted using 0.01N NaOH. 7, followed by hot water extraction at 80-90 ° C. for 24 hours, followed by purification by adding ethanol 2-4 times, and concentration and freeze-drying to obtain beta-glucan.

At this time, the yield and beta-glucan content vary according to the hot water extraction time, and as shown in Table 1 below, the yield and beta-glucan content increase rate decrease after 24 hours, so 24 hours is considered the optimal extraction time. do.

Mushroom Extract Solvent Classification extraction time transference number(%) Beta glucan content Echinacea Leaf Extract 6 4.13 ± 0.05 1.38 ± 0.02 12 4.42 ± 0.02 1.69 ± 0.04 20 4.89 ± 0.01 2.03 ± 0.02 24 5.99 ± 0.07 2.48 ± 0.07 48 6.26 ± 0.18 2.56±0.04 Hydrangea Leaf Extract 6 4.13 ± 0.02 1.35 ± 0.07 12 4.26 ± 0.02 1.64 ± 0.02 20 4.78 ± 0.16 2.00 ± 0.02 24 5.67 ± 0.03 2.39 ± 0.06 48 6.20 ± 0.02 2.41 ± 0.01 Green Tea Leaf Extract 6 3.99 ± 0.02 1.18 ± 0.01 12 4.19 ± 0.02 1.32 ± 0.02 20 4.41 ± 0.16 1.67 ± 0.02 24 4.98 ± 0.03 2.10 ± 0.08 48 5.17 ± 0.02 2.31 ± 0.06

Preparation Example 3. Preparation of Saw Palmetto

About 10 times as much purified water was added to 100 g of dried saw palmetto (purchased from Acen Korea Co., Ltd.) and reflux extraction was performed at 95-100 ° C. for 4 hours. After filtering the extract through a filter paper (Whatman, Dassel, Germany), it was concentrated under reduced pressure at 60°C using a conventional concentrator (EYELA, Japan). Then, the concentrate was freeze-dried to obtain saw palmetto extract.

Example 1.

Example 1-1. Antibacterial composition containing mushroom extract using fig enzyme, zinc mixture and echinacea leaf extract

Compared to the total weight of the total composition, 2 parts by weight of the fig enzyme extract of Preparation Example 1-1, 1.5 parts by weight of mushroom extract using the Echinacea leaf extract of Preparation Example 2-1, 0.3 parts by weight of zinc pyrithione, sodium polynaphthalenesulfonate An antimicrobial composition was prepared by mixing 3 parts by weight and the remaining parts by weight of purified water.

Example 1-2. Antibacterial composition comprising mushroom extract using fig enzyme, zinc mixture and hydrangea leaf extract

Compared to the total weight of the total composition, 2 parts by weight of the fig enzyme extract of Preparation Example 1-1, 1.5 parts by weight of mushroom extract using the hydrangea leaf extract of Preparation Example 2-2, 0.3 parts by weight of zinc pyrithione, sodium polynaphthalenesulfonate 3 An antibacterial composition was prepared by mixing parts by weight and the remaining parts by weight of purified water.

Example 1-3. Antibacterial composition containing mushroom extract using fig enzyme, zinc mixture and green tea leaf extract

Compared to the total weight of the total composition, 2 parts by weight of the fig enzyme extract of Preparation Example 1-1, 1.5 parts by weight of mushroom extract using the green tea leaf extract of Preparation Example 2-3, 0.3 parts by weight of zinc pyrithione, sodium polynaphthalenesulfonate 3 An antibacterial composition was prepared by mixing parts by weight and the remaining parts by weight of purified water.

Example 2.

Example 2-1. Antibacterial composition containing beta-glucan derived from mushroom extract using fig enzyme, zinc mixture and echinacea leaf extract

Compared to the total weight of the total composition, 2 parts by weight of the fig enzyme extract of Preparation Example 1-1, 1.5 parts by weight of beta-glucan derived from the mushroom extract using the Echinacea leaf extract of Preparation Example 2-4, 0.3 parts by weight of zinc pyrithione, sodium poly An antibacterial composition was prepared by mixing 3 parts by weight of naphthalenesulfonate and the remaining parts by weight of purified water.

Example 2-2. Antibacterial composition containing beta-glucan derived from mushroom extract using fig enzyme, zinc mixture and hydrangea leaf extract

Compared to the total weight of the total composition, 2 parts by weight of the fig enzyme extract of Preparation Example 1-1, 1.5 parts by weight of beta-glucan derived from the mushroom extract using the hydrangea leaf extract of Preparation Example 2-5, 0.3 parts by weight of zinc pyrithione, sodium polynaphthalene An antibacterial composition was prepared by mixing 3 parts by weight of sulfonate and the remaining parts by weight of purified water.

Example 2-3. Antibacterial composition containing beta-glucan derived from mushroom extract using fig enzyme, zinc mixture and leaf extract

Compared to the total weight of the total composition, 2 parts by weight of the fig enzyme extract of Preparation Example 1-1, 1.5 parts by weight of beta-glucan derived from the mushroom extract using the green tea leaf extract of Preparation Example 2-6, 0.3 parts by weight of zinc pyrithione, sodium polynaphthalene An antibacterial composition was prepared by mixing 3 parts by weight of sulfonate and the remaining parts by weight of purified water.

Example 3.

Example 3-1. Antibacterial composition containing mushroom extract using light-irradiated fig enzyme, zinc mixture and echinacea leaf extract

The fig enzyme extract of Preparation Example 1-1 was irradiated with light for 5 minutes using a Qraycam (AIOBIO, Seoul, Korea) equipped with a 405 nm wavelength LED.

2 parts by weight of the light-irradiated fig enzyme extract and 1.5 parts by weight of the mushroom extract using the echinacea leaf extract of Preparation Example 2-1, 0.3 parts by weight of zinc pyrithione, 3 parts by weight of sodium polynaphthalenesulfonate and the remaining parts by weight of purified water An antibacterial composition was prepared by mixing.

Example 3-2. Antibacterial composition comprising a mushroom extract using light-irradiated fig enzyme, zinc mixture and hydrangea leaf extract

The fig enzyme extract of Preparation Example 1-1 and the hydrangea leaf extract of Preparation Example 2-2 were irradiated with light for 5 minutes using a Qraycam (AIOBIO, Seoul, Korea) equipped with a 405 nm wavelength LED.

2 parts by weight of light-irradiated fig enzyme extract, 1.5 parts by weight of mushroom extract using light-irradiated hydrangea leaf extract, 0.3 parts by weight of zinc pyrithione, 3 parts by weight of sodium polynaphthalenesulfonate and the remaining parts by weight of purified water were mixed to prepare an antibacterial composition. manufactured.

Example 3-3. Antimicrobial Composition Containing Mushroom Extract Using Photoirradiated Fig Enzyme, Zinc Mixture and Green Tea Leaf Extract

The fig enzyme extract of Preparation Example 1-1 and the green tea leaf extract of Preparation Example 2-2 were irradiated with light for 5 minutes using a Qraycam (AIOBIO, Seoul, Korea) equipped with a 405 nm wavelength LED.

2 parts by weight of light-irradiated fig enzyme extract, 1.5 parts by weight of mushroom extract using light-irradiated green tea leaf extract, 0.3 parts by weight of zinc pyrithione, 3 parts by weight of sodium polynaphthalenesulfonate and the remaining parts by weight of purified water were mixed to prepare an antibacterial composition. manufactured.

Example 4.

Example 4-1. Antibacterial composition containing beta-glucan derived from mushroom extract using light-irradiated fig enzyme, zinc mixture and echinacea leaf extract

The fig enzyme extract of Preparation Example 1-1 was irradiated with light for 5 minutes using a Qraycam (AIOBIO, Seoul, Korea) equipped with a 405 nm wavelength LED.

2 parts by weight of the light-irradiated fig enzyme extract, 1.5 parts by weight of beta-glucan derived from the mushroom extract using the echinacea leaf extract of Preparation Example 2-4, 0.3 parts by weight of zinc pyrithione, 3 parts by weight of sodium polynaphthalenesulfonate, and the remaining parts by weight An antibacterial composition was prepared by mixing purified water.

Example 4-2. Antibacterial composition containing beta-glucan derived from mushroom extract using light-irradiated fig enzyme, zinc mixture and hydrangea leaf extract

The fig enzyme extract of Preparation Example 1-1 was irradiated with light for 5 minutes using a Qraycam (AIOBIO, Seoul, Korea) equipped with a 405 nm wavelength LED.

2 parts by weight of the light-irradiated fig enzyme extract, 1.5 parts by weight of beta-glucan derived from the mushroom extract using the hydrangea leaf extract of Preparation Example 2-5, 0.3 parts by weight of zinc pyrithione, 3 parts by weight of sodium polynaphthalenesulfonate and the remaining parts by weight of purified water By mixing to prepare an antibacterial composition.

Example 4-3. Antibacterial composition containing beta-glucan derived from mushroom extract using light-irradiated fig enzyme, zinc mixture and hydrangea leaf extract

The fig enzyme extract of Preparation Example 1-1 was irradiated with light for 5 minutes using a Qraycam (AIOBIO, Seoul, Korea) equipped with a 405 nm wavelength LED.

2 parts by weight of the light-irradiated fig enzyme extract, 1.5 parts by weight of beta-glucan derived from the mushroom extract using the green tea leaf extract of Preparation Example 2-6, 0.3 parts by weight of zinc pyrithione, 3 parts by weight of sodium polynaphthalene sulfonate and the remaining parts by weight of purified water By mixing to prepare an antibacterial composition.

Example 5.

Example 5-1. Antibacterial composition containing fig enzyme, zinc mixture, mushroom extract using echinacea leaf extract and saw palmetto extract

Compared to the total weight of the total composition, 2 parts by weight of the fig enzyme extract of Preparation Example 1-1, 1.5 parts by weight of mushroom extract using the Echinacea leaf extract of Preparation Example 2-1, 0.3 parts by weight of zinc pyrithione, sodium polynaphthalenesulfonate An antibacterial composition was prepared by mixing 3 parts by weight, 0.5 parts by weight of saw palmetto, and the remaining parts by weight of purified water.

Example 5-2. Antibacterial composition comprising fig enzyme, zinc mixture, mushroom extract and saw palmetto extract using hydrangea leaf extract

Compared to the total weight of the total composition, 2 parts by weight of the fig enzyme extract of Preparation Example 1-1, 1.5 parts by weight of mushroom extract using the hydrangea leaf extract of Preparation Example 2-2, 0.3 parts by weight of zinc pyrithione, sodium polynaphthalenesulfonate 3 An antibacterial composition was prepared by mixing parts by weight, 0.5 parts by weight of saw palmetto, and the remaining parts by weight of purified water.

Example 5-3. Antibacterial composition comprising fig enzyme, zinc mixture, mushroom extract and saw palmetto extract using green tea leaf extract

Compared to the total weight of the total composition, 2 parts by weight of the fig enzyme extract of Preparation Example 1-1, 1.5 parts by weight of mushroom extract using the green tea leaf extract of Preparation Example 2-3, 0.3 parts by weight of zinc pyrithione, sodium polynaphthalenesulfonate 3 An antibacterial composition was prepared by mixing parts by weight, 0.5 parts by weight of saw palmetto, and the remaining parts by weight of purified water.

Comparative Example 1.

The fig enzyme extract of Preparation Example 1 was extracted three times with a 5-fold weight n-hexane (hexane) solvent and then concentrated under reduced pressure to obtain a fig enzyme hexane fraction. Thereafter, the same extraction was performed with an ethyl acetate solvent to obtain a fig enzyme ethyl acetate fraction.

2 parts by weight of the fig enzyme ethyl acetate fraction of Preparation Example 1, 1.5 parts by weight of Echinacea extract of Preparation Example 2-1, 0.3 parts by weight of zinc pyrithione, 3 parts by weight of sodium polynaphthalenesulfonate and the rest, relative to the total weight of the total composition A composition was prepared by mixing parts by weight of purified water.

Comparative Example 2.

The manufacturing process is the same as that of Example 3-1, except that the fig enzyme extract was irradiated with light at a wavelength of 710 nm, which is an infrared ray rather than a wavelength of 405 nm.

Comparative Example 3.

The manufacturing process is the same as in Example 3-1, except that the fig enzyme extract was irradiated with light of a wavelength of 360 nm, which is an ultraviolet ray other than the 405 nm wavelength range.

Table 2 below shows the components of cosmetics (cleaners) prepared using Examples 1 to 5 and Comparative Examples 1 to 3. 6 parts by weight of lecithin, 3 parts by weight of caprylyl glycol, 3 parts by weight of caprylic/capric triglyceride, and 5 parts by weight of glycerin were mixed with the compositions of Examples and Comparative Examples, at a pressure of 1200 bar and a flow rate of 500 m/s. A stabilized liposome composition was prepared by passing it through a microfluidizer 5 times under the condition. Then, a detergent composition was prepared by mixing 20 parts by weight of a surfactant, 0.5 parts by weight of arginine, 0.4 parts by weight of an acidity regulator, and the remaining parts by weight of purified water with the same composition.

division Content (parts by weight) Purified water To 100 glycerin 5 lauryl betaine 4 Acrylate Copolymer 0.5 Sodium Cocoyl Glutamate 4 Decyl glucoside 4 Potassium Cocoyl Glycinate 4 sodium chloride 0.5 Spices One arginine 0.5 Dipropylene Glycol 0.05 Coconut Acid 0.2 Hydroxyacetophenone 0.5 Citric Acid 0.2 sodium isethionate 0.5 cellulose gum One sodium gluconate 0.5 caprylyl glycol 3 Caprylic/Capric Triglyceride 3 tea tree leaf oil 2 evening primrose oil 2 pine pine oil One Examples 1 to 5 or Comparative Examples 1 to 3 10

Experimental Example 1. Cytotoxicity evaluation

MTT cell viability assay (MTT Cell Viability Assay) was analyzed using the human epidermal epithelial cell line HaCaT. The cells were cultured in DMEM (Dulbecco's modified Eagle's media) medium containing 10% fetal bovine serum (Gibco) and 1% penicillin and streptomycin (Gibco) at 37° C. under 5% CO ₂ . The cells were subcultured once every 3-4 days. For sample treatment, HaCaT cells were dispensed into 24 wells at 1.0 X 10 ⁵ cells/Ml per well, cultured for 24 hours, washed twice with PBS, and 1ml of free serum medium was added to each well. After exchange, the samples were treated at a concentration of 50 to 200 ug/mL in each well. After 24 hours of incubation, MTT{3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide} reagent was treated with the culture solution at a concentration of 500 ug/mL per well, followed by reaction at 37℃ for 3 hours. The culture medium was removed. Then, formazan (purple) reduced by oxidoreductase of intracellular mitochondria was dissolved in DMSO and absorbance was measured at 570 nm to measure cell viability.

As a result, as shown in FIG. 1, cytotoxicity was not confirmed in Examples 1 to 5, and the results suggest that the samples are harmless to the human body and have excellent human safety.

Experimental Example 2. Evaluation of antibacterial efficacy

The antibacterial ability of the antimicrobial composition of the present invention was tested by disc diffusion assay. As test strains, Staphylococcus aureus , a gram-positive bacterium, and Escherichia coli , a gram-negative bacterium, were selected. Each strain was inoculated into LB liquid medium (Luria-Berani broth) and cultured at 37° C. for 24 hours. The culture medium was prepared by measuring the absorbance and diluting with 0.5 McFarland standard.

On the other hand, a solid medium was prepared by pouring Muller Hilton (MH) agar into a sterilized Petri dish to a thickness of 4 mm and cooling and solidifying. 100 ul of the aforementioned culture medium was evenly spread on MH solid medium. Thereafter, a paper disk was placed on the smeared MH medium, adsorbed with 50 ul of the fig fractions of Examples 1 to 5, and then incubated at 37 ° C for 24 hours to measure the diameter of the zone of inhibition.

division Restraint Diameter (mm) Staphylococcus aureus Escherichia coli Example 1-1 20 18 Example 1-2 21 22 Example 1-3 18 15 Example 2-1 22 21 Example 2-2 24 23 Example 2-3 20 18 Example 3-1 24 23 Example 3-2 23 23 Example 3-3 21 22 Example 4-1 27 24 Example 4-2 23 21 Example 4-3 22 25 Example 5-1 20 19 Example 5-2 21 22 Example 5-3 18 17 Comparative Example 1 17 15

As shown in Table 3, the antibacterial composition of the present invention formed an inhibition zone of up to 27 mm in Staphylococcus aureus medium and a 25 mm inhibition zone in E. coli medium, confirming its excellent antimicrobial ability.

Experimental Example 3. Evaluation of antifungal efficacy

The antibacterial ability of the antimicrobial composition of the present invention was tested by disc diffusion assay. As the test strain, ATCC 10231 Candida albicans was selected. Antifungal properties against Candida were measured by ASTM E2149 SHAKE FLASK TEST. 100 ul of the antibacterial composition of Example 2-1 was added to the culture medium in which Candida was cultured, and the reaction was carried out at 35 ° C for 1 hour.

division Initial bacterial count (CFU/mL) 1 hour later Antibacterial activity level (%) Example 2-1 21 22 99.2

As a result, as shown in Table 4, it was 2.8 x 10 ⁵ CFU/mL immediately after inoculation, but after 1 hour, a bacterial reduction rate of 99.2% was observed, and most Candida bacteria were removed, confirming excellent antibacterial activity (FIG. 2).

Experimental Example 4. Evaluation of antioxidant activity

The compositions of Examples 1 to 5 and Comparative Example were suspended in purified water to evaluate free radical scavenging activity.

The DPPH measurement method is a method of measuring absorbance at 540 nm to determine the degree of decolorization by scavenging the stable radical DPPH (2, 2-Diphenyl 1-picrylhydrazyl radical) by an inhibitor. Vitamin C was used as the control group, and a concentration (SC ₅₀ ) scavenging 50% of the radical DPPH was measured and compared with the control group.

[Equation 1]

DPPH scavenging activity (%) = {1-(Example or Comparative Example absorbance of sample treated group/absorbance of sample untreated group)} X 100

division SC ₅₀ (ug/mL) Example 1-1 55.5 Example 1-2 55.8 Examples 1-3 53.2 Example 2-1 53.1 Example 2-2 53.2 Example 2-3 49.9 Example 3-1 50.2 Example 3-2 52.2 Example 3-3 48.4 Example 4-1 48.1 Example 4-2 47.8 Example 4-3 45.5 Example 5-1 53.5 Example 5-2 53.8 Example 5-3 51.2 Comparative Example 1 69.7 Comparative Example 2 55.6 Comparative Example 3 55.4 control (vitamin C) 8.7

As a result, it can be seen that the antimicrobial composition of the present invention contains the beta-glucan component of the mushroom extract using the natural extract, and thus the antioxidant effect is remarkably improved compared to Comparative Example 1. In addition, it was confirmed that the antioxidant activity of the antimicrobial composition was significantly improved when irradiated with visible light between 400 and 700 nm, although the improvement in antioxidant effect was insignificant when irradiated with ultraviolet rays of 400 nm or less or infrared rays of 700 nm or more during light irradiation.

Experimental Example 5. Confirmation of skin moisturizing effect

For the hyaluronic acid contents assay, the human epidermal epithelial cell line HaCaT was used. The cells were cultured in DMEM medium (Dulbecco's modified Eagle's media) containing 10% fetal calf serum (Gibco) and 1% antibiotics (penicillin and streptomycin 50,000 units/mL, Gibco) at 37°C, 5% CO ₂ . . Cells were subcultured once every 3-4 days. For sample treatment, HaCaT cells were dispensed into 24 wells at 1.0 X 10 ⁵ cells/mL per well, cultured for 24 hours, washed twice with PBS, and 1 mL of free serum medium was added per well. After exchange, each sample was treated at a concentration of 100 ug/mL in each well. The cell culture medium cultured for 24 hours was collected and the hyaluronic acid concentration was measured using a hyaluronic acid ELISA kit (R&D Systems).

As a result, as shown in FIG. 3, the antibacterial compositions of Examples 1 and 5 showed an excellent increase in hyaluronic acid production compared to Comparative Example 1, which shows that the antibacterial composition of the present invention has excellent skin moisturizing effect.

Experimental Example 6. Confirmation of skin irritation

30 males aged 20 to 40 were selected, divided into 3 groups of 10 each, and used the male cleanser (Table 2) prepared using the compositions of Examples 1-1 to 5-1 for 10 days Thereafter, a survey was conducted on whether or not skin irritation occurred, and the results are shown in Table 6 below.

[Evaluation standard]

◎: no irritation at all

○: almost no irritation

△: There is slight irritation, so it is preferable not to use it on sensitive mucous membranes

X: Cannot be used due to severe stimulation

division Example 1-1 Example 2-1 Example 3-1 Example 4-1 Example 5-1 1 group ○ ○ ◎ ◎ ○ 2 groups ○ ○ ◎ ◎ ○ 3 groups ○ ○ ◎ ○ ○

According to Table 6, the male cleanser compositions of Examples 1-1 to 5-1 were generally evaluated as not having severe skin irritation, and in particular, Examples 3-1 and 4-1 showed the most skin irritation. Based on this, it can be seen that the antibacterial composition of the present invention uses ingredients harmless to the human body and is effective even for men's sensitive skin. Those skilled in the art will understand that it can be easily modified into other specific forms without changing the technical spirit or essential characteristics of the present invention. Therefore, the embodiments described above should be understood as illustrative in all respects and not limiting. For example, each component described as a single type may be implemented in a distributed manner, and similarly, components described as distributed may be implemented in a combined form.

The scope of the present invention is indicated by the following claims, and all changes or modifications derived from the meaning and scope of the claims and equivalent concepts should be interpreted as being included in the scope of the present invention.

Claims (11)

delete delete delete delete 0.1 to 3 parts by weight of fig enzyme extract irradiated with visible light between 400 and 700 nm for 1 to 60 minutes, 0.05 to 0.5 parts by weight of zinc pyrithione, 0.5 to 5 parts by weight of sodium polynaphthalenesulfonate and beta-glucan (β-glucan ) It relates to an antioxidant or antibacterial composition comprising 0.1 to 3 parts by weight,
The beta-glucan is prepared by mixing at least one plant leaf extract and alcohol selected from the group consisting of echinacea leaves, hydrangea leaves, and green tea leaves in a ratio of 1: 9 to 3: 7 and then immersing the zinnia mushroom extract is of origin,
Staphylococcus aureus ( Staphylococcus aureus ) Or Escherichia coli ( Escherichia coli ) To have an antimicrobial effect against, antioxidant or antibacterial composition. delete According to claim 5,
The composition can be used as a cosmetic, antioxidant or antibacterial composition. delete According to claim 5,
The composition can be used as a male cleanser, antioxidant or antibacterial composition. According to claim 5,
The composition further comprises skin irritation and erythema relief uses, antioxidant or antibacterial composition. a) A hot water extract of one or more plant leaves selected from the group consisting of echinacea leaves, hydrangea leaves, and green tea leaves and alcohol are mixed in a ratio of 1: 9 to 3: 7, and then the zinnia mushroom is immersed to prepare a zinnia mushroom extract step;
b) treating the zinnia mushroom extract with a degrading enzyme, followed by hot water extraction at pH 6 to 8 for 20 to 24 hours to obtain beta-glucan;
c) preparing a fig enzyme extract by pulverizing figs, preparing an extract with an ethanol solvent, and then repeating the process of removing ethanol with a vacuum concentrator 2 to 4 times:
d) irradiating the fig enzyme extract of step c) with visible light between 400 and 700 nm for 1 minute to 60 minutes; and
e) 0.1 to 3 parts by weight of the fig enzyme extract irradiated with visible light, 0.05 to 0.5 parts by weight of zinc pyrithione, 0.5 to 5 parts by weight of sodium polynaphthalenesulfonate, and beta-glucan obtained from the zinnia mushroom extract in step b). Mixing 0.1 to 2 parts by weight; antimicrobial composition manufacturing method comprising a.

Images