KR20110073947A - Cosmetic composition containing saw palmetto extract and production method thereof - Google Patents

Abstract

PURPOSE: A cosmetic composition containing saw palmetto extract is provided to ensure stability, anti-inflammatory effects, and moisturizing effects without side effects. CONSTITUTION: A cosmetic composition contains 0.10-10.0 weight% of saw palmetto extract. The cosmetic composition is manufactured in the form of essence, lotion, or nutrition cream. The cosmetic composition has effects of anti-inflammation, antibacterial, anti-fungi, or atopic dermatitis treatment. A method for preparing saw palmetto extract comprises: a step of washing, drying, and pulverizing saw palmetto fruits; a step of adding 100 weight parts of crushed saw palmetto fruits in a mixture solution of ethanol and distilled water and extracting at 55-65°C for 6-8 hours; and a step of adding crushed saw palmetto fruits to mixture solution of ethanol, butylene glycol, and distilled water and extracting at 55-65°C for 6-8 hours.

Description

Cosmetic composition containing saw palmetto extract, and a method for preparing the same {Cosmetic composition containing Saw Palmetto extract and Production Method Thereof}

The present invention relates to a cosmetic composition containing a saw palmetto extract. Preferably the saw palmetto extract relates to a cosmetic composition containing a saw palmetto extract, characterized in that 0.10 to 10.00% by weight based on the total weight of the cosmetic composition. In addition, the cosmetic composition relates to a cosmetic composition containing a saw palmetto extract, characterized in that for anti-inflammatory, antibacterial or anti-fungal, moisturizing effect, immune cell growth activity, erythema relaxation effect, atopic skin improvement effect.

And washing and drying the saw palmetto fruit with distilled water, and pulverizing; and extracting the pulverized product by hot water extraction or supercritical extraction, and then removing the insoluble material by filtration; and concentrating the extract under reduced pressure. It relates to a method for producing a saw palmetto extract, comprising; lyophilizing.

Allergy is derived from the Greek word allon argon, which means 'to try to react differently from normal', and in 1910, Burker is a term used by a pediatrician in a book to describe a person's ability to react changed action). In the case of allergies, even though the foreign substance is not really harmful, it causes a lot of symptoms in our body as a result of an over-immune reaction. In other words, the external intruder is harmless, but it is mistaken as an overreaction.

Allergies are basically classified as Types I to IV in the classification by Coombs and Gell. Among them, type I allergies are called "immediate type" allergies and cause atopic diseases. Type IV allergies are also called "delayed" allergies. Allergy type I is caused by an excessive immune response, and histamine-mediated reactions by IgE antibodies are typical and are divided into asthma, allergic rhinitis, atopic dermatitis, and hypersensitivity. Among the four diseases, atopic dermatitis is related to skin. .

The term atopy was first coined by Coca in 1923, and is a (no) + top (place) + y (ness), meaning 'weird' or 'inappropriate'. Refers to the nature of the tendency. Literally, it is common for a variety of causes to develop in complex pathways, and to relieve and relapse. Complex and diverse causes mean that treatment is difficult. These atopy postmarks are inherited and appear in family history. Allergic diseases caused by atopy predisposition include allergic dermatitis, allergic rhinitis, asthma, allergic conjunctivitis, and atopic dermatitis. These diseases may be present singly or simultaneously.

People with atopic inducers often have urticaria reactions in skin tests for protein substances frequently encountered in the external environment, such as house dust, mites, animal hair, food, pollen, and mold, and when eating or inhaling certain protein substances. Itching, skin attack, hives, angioedema, sneezing, runny nose, stuffy nose, conjunctival hyperemia, tears, etc.

In the mechanism of allergy to atopy, when allergens invade through the stratum corneum and reach the epidermal cells, macrophages, a type of white blood cells, are fed and broken down into pieces. If macrophages cannot be multi-processed due to the high amount of allergens invaded, information about the presence of unprocessed allergens is sent to T cells, the same type of white blood cells, and the T cells send the information back to eosinophils, a type of white blood cells. Eosinophils, which are widely distributed in the blood through white blood cells, are collected in the place where allergens invade. At this time, eosinophils accumulated in the affected area release excessive oxygen, causing inflammation, which is the cause of allergic reactions.

Symptoms of atopic dermatitis have severe itching, in the acute phase the skin is itchy, reddened, swollen or millet-like, small blisters or moist, scaby, itchy in the chronic phase, and the skin becomes hard, thick or thin. Scales, such as flakes or rice flour, appear to be sitting, blackened, or discolored white. Atopic dermatitis usually begins with infantile eczema, commonly referred to as fever.

Among the currently used therapeutic drugs, corticosteroids, which are corticosteroids, have anti-inflammatory and immunosuppressive effects and are excellent. However, long-term application of steroids can cause hair to shine on the skin, shrink the skin, reduce skin pigmentation, cause bacterial infections, acne, thin skin, and uneven skin. In severe cases, hormonal systemic symptoms may occur. In addition, stopping the drug causes the symptoms to explode again, making it impossible to stop the drug.

In addition, the skin of atopy patients is generally combined with bacterial infection as a result of long-term scratching and drying. More than 90% of atopic patients are infected with Staphylococcus aureus, which can be caused by scratching because the patient can't stand itching, but recent reports suggest that the toxins in these bacteria stimulate the body's immune system to cause allergic chemicals. It is said to make atopy worse. In other words, the bacteria themselves act as allergens. Therefore, the use of appropriate antibiotics is essential for the treatment of atopy.

Other medications include nonsteroidal ointments as moisturizers. In addition, sedatives or nerve stabilizers are used to soothe, and at night, an ointment is applied to moisturize and wrap the application area with a wrap. Therefore, the characteristics of the atopy, it can be seen that the development of a substance having an antimicrobial effect, moisturizing effect, anti-inflammatory effect as an essential item of the atopy treatment.

On the other hand, saw palmetto is a plant called saw palmetto, a kind of herb family. This fruit was used as an aphrodisiac by Indians living in the southeastern United States. They also cooked the fruit and used it as a medicine to pierce the nasal or clogged nose.

Recently, extracts from the fruit of saw palmetto have been used as medicinal and various health foods. Active research is being conducted in Europe and the United States as a natural food, and the greatest effect is anti-inflammatory action. Doctors such as US Read and Salomons We introduced the anti-inflammatory excellence of saw palmetto.

The fruit of saw palmetto contains oils containing many essential fatty acids and phytosterols. These fatty acids are Carpic, Caprylic, Caproic, Lauric, Palmitic and Oleic acids and their ethyl-esters. The main phytosterols are beta-sitosterol, Stigmasterol, Cycloartenol, Lupeol, Lupenone and 24-methyl-cyclo-artenol. The most important chemical components of saw palmetto fruit are vegetable sterols, fats, defatty acids, carotenoids, essential oils and polysaccharides. In the 1960s, phytosterols were detected by chromatography.

One study found that saw palmetto fruit contained vegetable sterols as compared to testosterone and 5-dihydro testosterone. These chemical constituents have been shown to have anti-inflammatory effects on the components of saw palmetto fruit by chemical bonding.

It is also assumed that the defatty acid and the ether ester components play a role in the inhibition of the 5-alpha-reducing agent. Pharmacological studies have shown anti-inflammatory effects on acid polysaccharides isolated from saw palmetto extract.

The prior art related to the present invention is a Korean patent registration 10-0863267 (composition for improving skin condition comprising ginseng, Astragalus, licorice, black axis, green tea, and aloe extract as an active ingredient), the herbal extract or herbal extract-containing as an active ingredient Zeolites are directed to compositions that inhibit the activity of intracellular tyrosinase and thereby effectively exert an excellent whitening effect that inhibits melanin production, wrinkle improvement efficacy, and skin damage caused by UV light.

In addition, Korean Patent Registration 10-0719761 (Atopic skin cosmetic composition containing goat's milk and sheep extract) relates to a cosmetic composition excellent in the safety on the skin and effective in the prevention or relief of atopic skin. However, these prior art is different from the present invention in the technical configuration.

Steroids are one of the side effects of the current drug used in atopic dermatitis. Long-term application may cause skin atrophy, less pigmented skin, bacterial infections, and acne. In severe cases, hormonal systemic symptoms may occur. In addition, stopping the drug causes the symptoms to explode again, making it impossible to stop the drug.

Antibiotics are also essential for the treatment of atopy. This is because the skin of atopic patients is followed by bacterial infection as a result of prolonged scraping and drying.

Therefore, in view of the characteristics of atopy as described above, it is an object of the present invention to develop a cosmetic composition having an atopic dermatitis improving effect with an antimicrobial effect, moisturizing effect, and anti-inflammatory effect as an essential item of an atopy treatment agent and a method for producing the same.

To solve the above problems, the present invention is a cosmetic composition containing a saw palmetto extract, preferably the saw palmetto extract cosmetics containing a saw palmetto extract, characterized in that 0.10 to 10.00% by weight relative to the total weight of the cosmetic composition To provide a composition.

The cosmetic composition may be a cosmetic composition containing a saw palmetto extract, characterized in that it has a formulation selected from the group consisting of essence, lotion, nourishing cosmetics, nutrition cream.

In addition, washing and drying saw palmetto with distilled water, and then pulverizing; and extracting the pulverized product by hot water extraction or supercritical extraction method, and then removing the insoluble material by filtration; and the extract was concentrated under reduced pressure, It provides a method for producing a saw palmetto extract comprising the step of lyophilization.

Through the cosmetic composition containing the present invention saw palmetto extract and its preparation method, anti-inflammatory and atopic skin improvement effect with excellent stabilization over time and less skin side effects can be expected for atopic dermatitis, which is recently increasing rapidly.

Furthermore, the present invention has an excellent effect on anti-fungal and antifungal effect, moisturizing effect, immune cell growth activity enhancing effect and erythema relaxation after primary skin stimulation.

The present invention relates to a cosmetic composition containing saw palmetto extract and a method for producing the same. More specifically, the cosmetic composition containing the saw palmetto extract, preferably, the saw palmetto extract provides a cosmetic composition containing the saw palmetto extract, characterized in that 0.10 to 10.00% by weight based on the total weight of the cosmetic composition. .

If the added amount is less than 0.1% by weight, no obvious anti-inflammatory and atopic skin improvement effect can be expected.If the content is more than 10% by weight, the anti-inflammatory and atopic skin improvement effect and product stability are not effective even though the content is increased. It is uneconomical.

The cosmetic composition may be a cosmetic composition containing a saw palmetto extract, characterized in that it has a formulation selected from the group consisting of essence, lotion, nourishing cosmetics, nutrition cream.

In addition, washing and drying the fruit of saw palmetto with distilled water, and pulverizing; and extracting the saw palmetto pulverized product, filtering to remove the insoluble material; and concentration of the extract under reduced pressure, lyophilizing It provides a method for producing a saw palmetto extract containing.

The method of extracting the saw palmetto pulverized product uses hot water extraction or supercritical extraction. The extraction solvent of the hot water extraction method is a mixture of ethanol and distilled water; Or a mixture of ethanol, butylene glycol and distilled water.

The hot water extraction method using a mixture of ethanol and distilled water is added to 1,400-1,600 parts by weight of a mixed solution of ethanol and distilled water with respect to 100 parts by weight of crushed saw palmetto fruit extract for 6-8 hours at 55 ~ 65 ℃. Preferably, the mixed solution uses ethanol and distilled water having a weight ratio of 3: 1.

The hot water extraction method using a mixture of ethanol, butylene glycol and distilled water is added to 1,400 ~ 1,600 parts by weight of a mixture of ethanol, butylene glycol and distilled water based on 100 parts by weight of crushed saw palmetto fruit 6 to 8 at 55 ~ 65 ℃ Extract for time. Preferably, the mixed solution uses ethanol, butylene glycol and distilled water is 2: 1: 1 by weight.

The hot water extraction method is a step of adding and sealing a supercritical extraction tank; and adding carbon dioxide to the extraction tank; and adding a mixed solution of 1,3-butylene glycol and 95% ethanol to the extraction tank. Step; and after increasing the temperature inside the extraction tank to 35 ~ 45 ℃, maintaining the 45 ~ 55 minutes at 290 ~ 310 atm; It provides a method for producing a saw palmetto extract, including; and after collecting the extract, dehydration, decolorization, deodorization step. Preferably, the mixed solution of 1,3-butylene glycol and 95% ethanol uses a weight ratio of 100: 5.

It demonstrates concretely based on an Example and a manufacture example below. But this

It is intended to illustrate the invention and is not intended to limit the scope of the invention thereto.

<Example 1> Preparation of extract of Saw Palmetto in the hot-water extraction method of the ethanol and distilled water as the extraction solvent

Saw palmetto berries native to the American Atlantic coast are imported, washed thoroughly with distilled water, and dried in a cool, dry place until there is no change in weight, followed by a homogenizer (AM-7, Hyronchem). Trituration with.

To 100 g of the pulverized product was added 1500 g of a mixed solution mixed with ethanol and distilled water in a weight ratio of 3: 1, and extracted at 60 ° C. for 7 hours.

The extract was first filtered through a 200 mesh filter cloth (Cheongggye Co., Ltd.), and the second filtration was filtered using Whatman No. 2 filter paper at room temperature to remove insoluble matters. Then, the resultant was concentrated under reduced pressure at 50 ° C. in a distillation apparatus equipped with a cooling capacitor and lyophilized to obtain a saw palmetto extract having a dry weight of 20.4 g.

<Example 2> Preparation of Saw Palmetto extract by hot water extraction of the ethanol, butylene glycol and distilled water as the extraction solvent

To 100 g of crushed beef palmetto prepared in Example 1, 1500 g of a mixed solution of ethanol, butylene glycol and distilled water in a weight ratio of 2: 1: 1 was added thereto, and extracted at 60 ° C. for 7 hours. Primary filtration was carried out with a 200 mesh filter cloth (Cheongggye Sangpo), and the secondary filtration was filtered with Whatman No. 2 filter paper at room temperature to remove insoluble matters. Then, the resultant was concentrated under reduced pressure at 50 ° C. in a distillation apparatus equipped with a cooling capacitor, and then lyophilized to obtain a saw palmetto extract having a dry weight of 21.2 g.

<Example 3> Preparation of a second extract, Saw Palmetto with Supercritical Extraction

600 g of the pulverized product of bopalmeto prepared as in Example 1 was introduced into a supercritical extraction tank having an internal volume of 1.2 L, sealed and introduced into a supercritical extraction tank using a high pressure gas pump, and carbon dioxide was supplied to the extraction tank to increase the pressure.

At the same time, a mixture of 1,3-butylene glycol and 95% ethanol, a water soluble cosolvent, was introduced into the extraction tank at the rate of 1.1 g / min using a high pressure liquid pump. The mixed solution was used a weight ratio of 100: 5.

When the pressure reaches 300 atm, the supply of carbon dioxide and cosolvent is stopped, and the temperature inside the extraction tank is increased to 40 ° C at a rate of 0.5 ° C / min using a heating wire surrounding the outside of the extraction tank using a PID (Proportional Integral Derivative) temperature controller. It heated up. The pressure of the extraction tank due to the elevated temperature was partially discharged by using a pressure regulator attached to the outlet of the extraction tank to adjust the pressure to 300 atm, and the temperature and pressure of the extraction tank were maintained at 40 ° C. and 300 atm for 50 minutes.

The high pressure gas pump was restarted to continuously supply carbon dioxide to the extraction tank at a flow rate of 20 L / min, and the high pressure liquid pump was restarted to supply a water-soluble cosolvent at a rate of 1.1 g / min. At the same time, the extract was discharged using a pressure regulator attached to the outlet and the temperature and pressure of the extraction tank were kept constant.

Due to the pressure and temperature drop released through the pressure regulator, carbon dioxide is transferred to the solid dry ice, which causes clogging of the tube on the final outlet side. Thus, the temperature of the pressure regulator was maintained at 70 ° C. The extracted extract was decompressed to atmospheric pressure to release carbon dioxide into the atmosphere, and the water-soluble active ingredient and cosolvent of the raw material were collected together into the collector.

After the process was carried out for 6 hours, the addition of the aqueous cosolvent was stopped and the extraction operation was terminated. After extraction, dehydration, decolorization, and deodorization were performed to obtain 142 g of the final saw palmetto extract.

Test Example 1 Anti-inflammatory Effect Measurement

In order to examine the anti-inflammatory effects on the present Saw Palmetto extract, the left ear of the mouse and the right ear of the test site were cleaned with ethanol before applying the sample. 1 hour after the last application, ethanol was applied to the left ear and arachidonic acid (2mg / ear) to the right ear. After 1 hour, the ear edema (ear e ma) was micrometered on both ears three times. Repeated measurements were taken.

The anti-inflammatory effect was determined as the degree of edema inhibition based on the arachidonic acid treatment group.

............[수학식 1]

Equation 1

 A: Average thickness of control ears (thickness of arachidonic acid-treated ears-thickness of untreated ears)

 B: Thickness of sample-coated group ears (thickness of sampled ears-thickness of untreated ears)

TABLE 1

sample density(%) menstruum Ear thickness (㎛) % Inhibition Before sample processing After sample processing Arachidonic acid 2mg / ear ethanol 296 547 - Example 1 Extract 0.5 ethanol 289 502 15.1 Example 1 Extract 1.0 ethanol 279 484 18.3 Example 1 Extract 2.0 ethanol 293 492 20.7 Example 2 Extract 0.5 ethanol 285 495 16.3 Example 2 Extract 1.0 ethanol 295 498 19.1 Example 2 Extract 2.0 ethanol 287 483 21.9 Example 3 Extract 0.5 ethanol 274 479 18.3 Example 3 Extract 1.0 ethanol 284 478 22.7 Example 3 Extract 2.0 ethanol 294 478 26.7

As can be seen from Table 1, the saw palmetto extract shows an excellent inflammation inhibition rate than the arachidonic acid used as a treatment group,

In particular, it was found that the sample extracted by the supercritical extraction method was superior to the sample extracted by the hot water extraction method.

Test Example 2 Antibacterial and Antifungal Effect Measurement

Saw palmetto extract The antimicrobial and antifungal effects were measured using the extracts of Examples 1, 2 and 3.

Antimicrobial and antifungal experiments were measured by agar diffusion using a paper disc. The strain was inoculated with 100 ml of platinum in a liquid medium and incubated for 48 hours at 37 ° C (Table 2).

100 μl of the strain culture was inoculated (107-108 cfu / ml) on top agar (0.75% agar) plate and applied onto the plate. Saw palmetto extract was injected into a sterile paper disc at 20 μl and dried, and the disc was placed on the plate surface. 36 hours of incubation and antimicrobial and antifungal activity was measured by the clear zone (clear zone) generated around the agar plate.

As strains, the following microbial test strains were used; Candida albicans (ATCC 10259) Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 29737) Bacillus subtilis (ATCC 6633), the growth medium of the strain is usually Agar (nutrient agar) or trypticase soy agar (Difco) was used and the culture conditions for each strain are shown in Table 2. The experimental results are shown in Table 3 below.

Table 2 (culture conditions for each strain)]

number Strain badge type One Candida Albican Common agar Exhalation 2 Escherichia coli Tryticase Bean Agar Exhalation 3 Staphylococcus aures Common agar Exhalation 4 Bacillus subtilis Common agar Exhalation

[Table 3]

Strain Escherichia coli Staphylococcus
Aures Bacillus
Subtilis Candida
Albican extract
Concentration (% by weight) Example 1 Example
2 Example
3 Example 1 Example
2 Example
3 Example 1 Example
2 Example
3 Example 1 Example
2 Example
3 0.5 3 4 6 3 5 7 4 4 6 3 4 5 1.0 5 6 8 6 6 9 5 6 8 5 5 8 3.0 6 7 9 7 8 10 6 7 10 7 7 9 5.0 8 9 11 8 9 12 7 8 11 8 9 12 10.0 9 9 15 10 11 14 10 10 16 9 10 14

As can be seen in Table 3, saw palmetto extract showed an excellent antibacterial activity, in particular, the extract extracted by supercritical extraction method had better antibacterial and antifungal effects than the extract extracted by hydrothermal extraction method, and had antibacterial and antifungal effects in a concentration-dependent manner. I could see that.

<Test Example 3> Moisturizing effect measurement

The moisture of the extract of Examples 1, 2, 3 was measured by the following method using a moisture meter [Corneometer CM 825, Courage + Kazaka Corporation].

Twenty healthy women in their 30s are washed several times with water in the upper arm 2 × 2 ㎠ and 8 compartments, and the water is removed without irritation. After 5 minutes, purified water, the extract of Example 1, the extract of Example 2, and the extract of Example 3 were evenly applied by dropping 10 µl of each of the two compartments. After 10 minutes, five measurements were taken per compartment using a coronometer. It was measured by the following equation six times at intervals of 5 minutes for one compartment, and the data values are shown in Table 4 as the average value for 20 people. The measurement conditions were maintained at a constant temperature and humidity of room temperature 20 ℃, relative humidity 30%.

    Moisture content (%) = Moisture meter after sample application-Moisture meter before sample application

[Table 4]

sample

Minutes Purified water Example 1 Extract Example 2 Extract Example 3 Extract 10 25 28 30 31 15 14 17 21 26 20 8 10 13 23 25 4 6 9 21 30 2 5 7 18 35 One 3 4 17 40 0 2 3 17

As can be seen in Table 4, the saw palmetto extract has excellent water retention ability over time than the control purified water, and also shows that the extract of Example 3 is more excellent in moisturizing power than the extracts of other examples 1 and 2. Could. Therefore, saw palmetto extract was able to prove that the moisturizing effect is excellent.

Test Example 4 Measurement of Growth Activity of Immune Cells

MTT assay is a method widely used for cell proliferation and toxicity by measuring the number of living cells. The living cells are water-soluble and yellow salts of MTT (3- [4,5-dimethylthiazole-2-yl]-in the mitochondria. 2,5-diphenyltetrazolium bromide) is used to reduce the water-insoluble blue formazan derivative by succinate dehydrogenase.

After the dissolving agent was added to the formazan derivative, the absorbance was measured. In more detail, Raji (Human B cell) and Jurkat (Human T cell), human immune cells, were analyzed by MTT (3- [4,5-dimethylthiazole-2-yl] -2,5-diphenyltetrazolium bromide) assay. It was carried out by.

Cell growth was incubated in 37%, 5% CO ₂ incubator using 10% FBS in RPMI1640 (GIBCO, USA) medium. In the measurement of immune cell growth activity, each sample was applied to each sample after 24 hours of incubation with a cell concentration of 4 × 10 ⁴ cells / ml in a 24 well plate.

After 48 hours of incubation, discard the medium in each well, add 1000 μl of MTT solution per well, remove MTT after 4 hours, add 1000 μl of DMSO per well, incubate at 37 ° C. overnight, and measure absorbance at 570 nm. It was. In this case, the experiment was conducted in the same manner without the addition of saw palmetto extract as a control to measure the difference in relative absorbance over a total of 8 days to observe the growth activity of the cells. The results are shown in Table 5.

TABLE 5

Example Of saw palmetto extract in the reaction solution
Final concentration (㎍ / ㎖) Growth activity (%) B cell T cell Example 1 200.0 101 102 Example 2 200.0 104 103 Example 3 200.0 112 119

As shown in Table 5, the results of measuring the growth activity of immune cells using Raji (Human B cell) and Jurkat (Human T cell), which are human immune cells, showed the best growth activity when the extract of Example 3 was applied. It was found to increase the activity.

Preparation of <Production Example 1> Essence (essence)

After dividing and measuring the water phase and the oil phase according to the composition, it was mixed and dissolved separately at 80 ± 2 ℃, the oil phase was mixed with the water phase. Subsequently, the raw material 13 which is a neutralizing agent was added and neutralized, and it cooled to 45 degreeC. After cooling, the raw material 14 was added and cooled to 35 ° C to prepare an essence.

division Raw material name weight(%) Prescription 1 Prescription 2 Prescription 3 Number


Prize One  Purified water Balance Balance Balance 2  Saw palmetto extract of Example 1 5.000 - - 3  Saw palmetto extract of Example 2 - 5.00 - 4  Saw Palmetto Extract of Example 3 - - 5.00 5  Carbomer 0.10 0.10 0.10 6  Disodium ID 0.05 0.05 0.05 7  Butylene glycol 3.50 3.50 3.50 U
Prize 8  Natural Preservative 0.01 0.01 0.01 9  Macadamia Nut Oil 4.00 4.00 4.00 U

Prize 10  Mineral oil 3.00 3.00 3.00 11  The Mechicon 1.00 1.00 1.00 12  Caprylic Capric Triglyceride 2.00 2.00 2.00 Chinese 13  Triethanolamine 0.25 0.25 0.25 incense 14  Spices 0.01 0.01 0.01

* Comparative Preparation Example 1 was prepared in the same manner as in Formulation 3 of Preparation Example 1 except that purified water was used instead of the saw palmetto extract by the supercritical extraction method of Example 3.

Test Example 5 Measurement of Erythema Efficacy Effect After Primary Skin Stimulation (Closed Closure Experiment)

Formula 1, 2, 3 and Comparative Preparation Example 1 was applied to the skin stimulant sodium lauryl sulfate (Sodium Laurly Sulfate) so that 0.5%, and to confirm the erythema relief effect was carried out the following clinical confirmation experiment. The subjects were 20 women in their 20s who were judged to have no current skin disease without atopic dermatitis and other eczema.

The erythema index (E-index) is measured before application of the sample to the inside of the forearm of both sides of the subject. The patch was attached during. The patch was removed and the erythema index was measured after the subject exposed the test site in a space without air movement and without direct sunlight, and then stabilized for 30 minutes.

After the measurement using Mexameter (Courage + Khazaka), the results are shown in Table 6. The temperature was kept constant at a room temperature of 23 ° C. and a relative humidity of 60%.

TABLE 6

sample BL DAY0 DAY1 DAY2 WEEK1 Sodium Lauryl Sulfate 0.5% 210 340 309 273 256 Prescription 1 211 315 289 250 224 Prescription 2 211 312 280 239 217 Prescription 3 210 285 254 226 212 Comparative Production Example 1 209 329 293 264 248

As a result of Table 6, it can be seen that the skin erythema-releasing effect is superior to Comparative Preparation Example 1 prepared without adding the cosmetics of Formulations 1, 2, and 3 prepared by adding saw palmetto extract.

Experimental Example 6 Measurement of Clinical Evaluation of Atopic Skin Improvement Effect

The clinical evaluation of the atopic skin improvement effect of the formulations 1 to Comparative Example 1 of Preparation Example 1 was measured. In order to evaluate the atopic skin improvement effect of Formulation 1 to Comparative Preparation Example 1 of Preparation Example 1, the following experiment was performed on 20 small children who were treated in hospitals and acupuncture hospitals with atopic skin. The test sample was applied to the whole body by dividing the right side and the left side by the double-blinded test, but the use of other moisturizers that could affect the effect of the test sample was prohibited. After 1, 2, 3, 4 weeks of application of the test sample was evaluated by SCORAD SCORAD (SCORing Atopic Dermatitis) measurement results are shown in Table 7 below.

 1) Criteria

  ① Extent criteria: Area = damage area / 100

  ② Intensity criteria

    -Erythema (1/2/3)

    -Edema (1/2/3)

    -Exudate (1/2/3)

    -Skin peeling (1/2/3)

    Taekwondo degree (1/2/3)

    -Drying degree (1/2/3)

  Subjective criteria

    -Itching (1-10)

    Insomnia (1-10)

    * Scorad calculation = (accuracy criterion / 5) + (strength criterion / 7) + subjective criterion

TABLE 7

Classification Week 0 1 week 2 weeks 3 weeks 4 Weeks Prescription 1 62.4 43.2 30.7 24.8 19.5 0% 30.8% 50.8% 60.3% 68.8% Prescription 2 65.3 43.1 31.4 25.6 18.9 0% 34.0% 51.9% 60.8% 71.1% Prescription 3 61.7 32.8 17.5 11.6 8.3 0% 46.8% 71.6% 81.2% 86.5% Comparative Production Example 1 63.2 47.9 42.7 38.1 36.5 0% 24.2% 32.4% 39.7% 42.2%

As can be seen in Table 7, the prescription example containing the saw palmetto extract prepared in Formulations 1 to 3 of Preparation Example 1 can be seen that the atopic skin improvement effect is apparent, very excellent compared to Comparative Preparation Example 1 I could confirm that.

In particular, the results after 4 weeks showed an improvement of more than 68%, especially Formula 3 containing saw palmetto extract extracted by supercritical extraction showed an excellent improvement of about 86%.

Meanwhile, in order to examine the atopic dermatitis alleviating effect felt by the caregiver, after using the cosmetic preparation of Formulation 1 to Comparative Preparation Example 1 of Preparation Example 1 for one month, the parents of the user according to the following criteria, itching, erythema change, dry skin It is shown in Table 8 to evaluate the degree of improvement of the dermatitis and the side effects caused by the test sample, along with the satisfaction with.

[Criteria]

One 2 3 4 5 no effect Some usually Great Very good Very dissatisfied dissatisfaction usually satisfied very good

[Table 8]

Classification Itching of the skin Erythema change Dry skin Overall improvement Side Effect Prescription 1 4.5 4.5 4.0 4.0 4.0 Prescription 2 4.0 4.5 4.0 4.0 4.0 Prescription 3 4.5 4.5 4.5 4.5 4.0 Comparative Preparation Example 1 2.5 2.5 3.5 3.0 3.5

As can be seen in Table 8, Formulas 1 to 3 according to the present invention was confirmed to have an improvement effect in all evaluation items for a person having atopic skin as a test subject.

Preparation Example 2 Preparation of Flexible Lotion

The aqueous phase and the ethanol phase were separated and weighed according to the following composition, and then mixed and dissolved separately, and the ethanol phase was mixed with the aqueous phase. The additives were then added and mixed. This was filtered using a membrane filter (Samdeok filtration company, paper filter) to prepare a flexible cosmetic water.

division Raw material name weight(%) Prescription 1 Prescription 2 Prescription 3 Number
Prize One  Purified water Balance Balance Balance 2  Saw palmetto extract of Example 1 5.00 - - Number


Prize 3  Saw palmetto extract of Example 2 - 5.00 - 4  Saw Palmetto Extract of Example 3 - - 5.00 5  glycerin 1.00 1.00 1.00 6  Sodium PCA Liquid 3.00 3.00 3.00 egg
nose
Prize 7  Modified alcohol 10.00 10.00 10.00 8  Natural Preservative 0.01 0.01 0.01 First
end
My 10  Spices 0.01 0.01 0.01

Production Example 3 Preparation of Nutritional Lotion

After dividing and measuring the water phase and the oil phase according to the composition, it was mixed and dissolved separately at 80 ± 2 ℃, the oil phase was mixed in the water phase. Subsequently, the raw material 14 which is a neutralizing agent was added and neutralized, and it cooled to 45 degreeC. After cooling, the raw material 15 was added and cooled to 35 ℃ to prepare a nourishing lotion.

division Raw material name weight(%) Prescription 1 Prescription 2 Prescription 3 Number


Prize One  Purified water Balance Balance Balance 2  Saw palmetto extract of Example 1 5.00 - - 3  Saw palmetto extract of Example 2 - 5.00 - 4  Saw Palmetto Extract of Example 3 - - 5.00 5  Carbomer 0.15 0.15 0.15 6  Disodium II 0.02 0.02 0.02 7  Butylene glycol 1.00 1.00 1.00 U


Prize 8  Natural Preservative 0.01 0.01 0.01 9  Macadamia Nut Oil 2.00 2.00 2.00 10  Mineral oil 6.00 6.00 6.00 11  Dimethicone 2.00 2.00 2.00 12  Sorbitan sesquioleate 0.40 0.40 0.40 13  Polysorbate 60 1.60 1.60 1.60 medium
anger 14  Triethanolamine 0.15 0.15 0.15 incense 15  Spices 0.01 0.01 0.01

Production Example 4 Preparation of Nutritious Cream

After dividing and measuring the water phase and the oil phase according to the composition, it was mixed and dissolved separately at 80 ± 2 ℃, the oil phase was mixed with the water phase. Subsequently, the raw material 12 which is a neutralizing agent was added and neutralized, and it cooled to 45 degreeC. After cooling, the raw material 13 was added and cooled to 35 ℃ to prepare a nutrient cream.

division Raw material name weight(%) Prescription 1 Prescription 2 Prescription 3 Number


Prize One  Purified water Balance Balance Balance 2  Saw palmetto extract of Example 1 5.00 - - 3  Saw palmetto extract of Example 2 - 5.00 - 4  Saw Palmetto Extract of Example 3 - - 5.00 5  Disodium ID 0.05 0.05 0.05 6  Hyaluronic Acid Extract 2.50 2.50 2.50 7  glycerin 8.00 8.00 8.00 8  Carbomer 0.20 0.20 0.20 U


Prize 9  Natural Preservative 0.01 0.01 0.01 10  lecithin 0.60 0.60 0.60 11  Macadamia Nut Oil 10.0 10.0 10.0 medium
anger 12  Triethanolamine 0.15 0.15 0.15 incense 13  Spices 0.01 0.01 0.01

In recent years, while allergic diseases are rapidly increasing, the effectiveness of steroids among the currently used therapeutic drugs, but there is a problem that the side effects are large. In addition, atopic dermatitis is commonly accompanied by bacterial infection, so antibiotics have been commonly used.

Accordingly, the present invention has an anti-inflammatory and atopic skin improvement effect by providing a cosmetic composition and a method for producing the same, which contains a saw palmetto extract of natural medicinal plant resources. Furthermore, the present invention has excellent antimicrobial effect, moisturizing effect, anti-inflammatory effect, as well as stability over time, and low side effects of the industry.

1 shows a manufacturing process of the present invention.

Claims (5)

Cosmetic composition containing saw palmetto extract (Saw Palmetto extract). The cosmetic composition according to claim 1, wherein the content of the saw palmetto extract is 0.10 to 10.00 wt% based on the total weight of the cosmetic composition. The cosmetic composition of claim 1, wherein the cosmetic composition has a formulation selected from the group consisting of essence, lotion, nourishing cream and nourishing cream. According to any one of claims 1 to 3, wherein the cosmetic composition is any one or more from the group consisting of anti-inflammatory, antibacterial or anti-fungal, moisturizing effect, immune cell growth activity, erythema relaxation effect and atopic skin improvement effect Cosmetic composition containing saw palmetto extract, characterized by the effect. Washing and drying saw palmetto fruit with distilled water, and then grinding; Method of extracting 1,400-1,600 parts by weight of a mixed solution of ethanol and distilled water in a weight ratio of 3: 1 to 100 parts by weight of crushed saw palmetto fruit extract for 6-8 hours at 55 ~ 65 ℃ ;, saw palmetto fruit 1,400-1,600 parts by weight of a mixed solution of ethanol, butylene glycol and distilled water in a weight ratio of 2: 1: 1 based on 100 parts by weight of the crushed product was added and extracted at 55-65 ° C. for 6-8 hours; And 600 parts by weight of ground crushed powder of saw palmetto fruit in a supercritical extraction tank and sealed, carbon dioxide is added to the extraction tank, a mixture of 1,3-butylene glycol and 95% ethanol 100: 5 by weight To the extraction tank, the temperature inside the extraction tank to 35 ~ 45 ℃ temperature, and maintained for 45 to 55 minutes at 290 ~ 310 atm; extraction method; extraction using any one method selected from the group consisting of step; Filtering the extract of saw palmetto fruit to remove insoluble matters; And Concentrating the filtered saw palmetto extract under reduced pressure, lyophilizing; manufacturing method of saw palmetto extract comprising a.

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