KR100982883B1 - Cosmetic composition comprising mixture extract and preparation thereof - Google Patents

Abstract

The present invention includes a mixed extract of Dandelion ( Taraxacum officinlale ), Perilla ocymoides , Participle (Gloiopeltis Tenax), Neilbo Nucifera Seed, Cestrum nocturnum and Eriobotrya Japonica Leaf. It provides a cosmetic composition and a method of manufacturing the same. The cosmetic composition of the present invention has an effect of antibacterial, antioxidant, whitening, moisture retention, and is useful as a cosmetic for improving atopic skin.

Dandelion, Pleurotus eryngii, Participating fern, Lotus root, Scented aroma, Loquat leaf, Antibacterial agent, Cosmetics, Atopy

Description

Cosmetic composition comprising a mixed extract and a method for preparing the same {Cosmetic composition comprising mixture extract and preparation etc}

The present invention relates to a cosmetic composition comprising a mixed extract and a preparation method thereof.

The etymology of atopy is the Greek term "I burn", which can represent the itching of an atopian at once. As the term atopy means "weird" or "inappropriate", it occurs mainly in infancy and appears in the form of fever, but its cause has not been clearly identified. This is caused mainly by the decrease in the amount of ceramides in the stratum corneum. As the moisture retention function of the stratum corneum decreases, the skin dries and the stratum corneum occurs easily. As the skin barrier function deteriorates, the stimulus-inducing substance enters the stratum corneum. Invasion causes irritation and skin allergies easily occur as antigen proteins are difficult to invade in normal skin, resulting in pruritus symptoms that are difficult to tolerate. Therefore, more than 90% of atopic patients are infected with Staphylococcus aureus, which may be caused by scratching because the patient cannot tolerate itching, but recent reports show that the toxins of this bacterium stimulate the body's immune system and cause allergies. It turns out chemicals that make atopy worse. In other words, the bacteria themselves act as allergens.

Atopic dermatitis is increasing worldwide, resulting in decreased water retention, loss of skin protection, and allergic reactions due to sensitive stimuli, which are caused by free radicals. By causing atopic skin. It can also get worse by infecting bacteria, viruses, and fungi. Therefore, in order to treat such atopy, it is essential to develop an excellent antimicrobial, moisturizing and anti-inflammatory effect, no side effects and low cost, and easy-to-use cosmetics containing the material.

Danax ( Taraxacum officinlale ) is an asteraceae plant, one of the six major herbs of Chinese medicine, and has been reported as a plant that is good for antibacterial and anti-inflammatory. According to classical literature such as Dongbobogam, it is a folk remedy that cleans and removes boils. It was also used. Korean Patent Publication No. 195-005739 describes the antibacterial atopy treatment effect of dandelion.

Perilla ocymoides is an annual herb belonging to the Lamiaceae family. It is used in the late summer by cutting out the outpost in the shade. Its taste is spicy and warm, and it acts on menopause, parenteral and stomach diameter. In addition, sweating eliminates the wind, make the stomach well, stabilize the fetus, detoxify the fish poisons, pharmacological experiments revealed weak fever, health, fungal, antiseptic, etc. In addition, self-leaving lobe is used for abundant signs, gastrointestinal bloat, fullness, vomiting and diarrhea, coughing and shortness of breath, and vomiting of pregnant women, anxiety due to gas, and fish poisoning. Korean Patent Publication No. 2002-0061963 describes the antibacterial atopy treatment effect of the lobule.

Participants (Gloiopeltis Tenax) belongs to the red algae among edible algae that are used for food. According to a study on the physiological activity effect of Participating ferns of Korean Journal of Nutrition, 39 (4), 366 ~ 371, 2006 Has been reported.

Nelumbo Nucifera Seed is the fruit of lotus, also called lotus or lotus, and is rich in protein, fat, carbohydrates, vitamins and minerals. Yeonjagi is a weak function of the diarrhea when diarrhea is a function of stopping the diarrhea by the (,) function, and the earthenware (자 子) and antler (鹿 茸) together with the god (腎) It is used for the treatment of oil wells (몽 精) and dream tablets (해서 精) caused by weak function, and is used in the treatment of the white crab with the encyclopaedia (白果) and golden cherry tree (자 子). In addition, Sanjoin (酸棗仁), Baekmun-dong (麥 門冬) along with the bleeding (陰血) is confused, the heart beats well, is used to treat the symptoms of sleep.

Cestrum nocturnum is said to have the effect of reducing heat to native plants in South America and the West Indies, so it is good for cold throats or fever.

Eriobotrya Japonica Leaf is known to contain components such as amigdaline, uroleic acid, olenolic acid, tannin, and vitamin B1. In addition, the loquat leaf is described as a prescription medicine for treating regurgitation and vacancies in "Odor odors" <the main posterior region>, and is described as a tree that improves various cancers.

Currently, to treat atopic dermatitis, moisturizers or taricides are used, or steroid preparations such as triamcinolone and fluocinolone, antibiotics such as clindamycin, dicloxacilline, antipruritic agents, potassium permanganate, cetrimide, chlorhexidine, chloroxylenol, dibromopropamidine, polynoxylin, Antibiotics such as povidone iodine and triclosan, immunomodulation, immunosuppression, immunotherapeutic, food, fragrance, and herbal medicine are used. However, tar and steroid preparations have serious side effects such as rashes, skin color changes, high blood pressure, cataracts, and UV treatments, which cause sunburn, itching, pigmentation and prolonged treatment and high risk of premature aging and cancer. Antibiotics have the disadvantage of being mainly used as oral medicines. In addition, the antimicrobial agent has a skin irritation with concentration. Immune modulators, inhibitors, etc. are most commonly used in recent years, but there are difficulties in using them such as those that should not be used on faces infected with viruses such as chickenpox and shingles. In addition, food therapy, herbal treatment, and other fragrance therapy has no side effects on use, but the effect is often insignificant, and the cosmetics for skin coating depend on imports, so the price is expensive and there is a limitation in use. Therefore, there is an urgent need for research on ingredients having excellent antibacterial and atopic skin improvement effects using domestic native plants.

It is an object of the present invention to provide a cosmetic composition comprising an extract that is excellent in anti-inflammatory, antioxidant, antibacterial, cell proliferation, skin itching, atopic skin improvement, and the like without any problems in stability and safety.

Another object of the present invention to provide a method for producing a cosmetic composition comprising an extract having such an effect.

The present invention provides a cosmetic composition comprising a mixed extract of dandelion, self-leaving, fern, lotus root, scented and loquat leaves.

In the cosmetic composition of the present invention, the mixed extract may be a mixture of dandelion extract, cotyledon extract, participating extract, lotus root extract, scent extract and loquat leaf extract in various ratios. For example, the mixed extract of the present invention is dandelion extract: cotyledon extract: fern extract: lotus root extract: scent extract: loquat leaf extract 1: 0.5 ~ 3: 0.5 ~ 5: 0.5 ~ 5: 0.2 ~ 2: It is preferable to mix in 0.5 to 5 weight ratio, and it is more preferable that it is 1: 1: 2: 2: 0.5: 2 weight ratio.

The mixed extract of the present invention may be extracted using various solvents by various extraction methods known to those skilled in the art. For example, in the present invention, the mixed extract is a mixture of dandelion, purple leaf, participating fern, lotus root, scented and loquat leaf extracted with a solvent selected from water, anhydrous alcohol having 1 to 4 carbon atoms or hydrous alcohol and ethyl acetate, respectively. It may be, or the extract extracted with the solvent after mixing in various ratios of dandelion, self-leaving, participating fern, lotus root, scented and loquat leaves in various ratios.

As a result of comparing the extracts extracted by different solvents during the extraction of the dandelion, self-leaving, participating fern, lotus root, scented and loquat leaf, there was a slight difference in the amount extracted according to different solvents, but the extraction components were the same. It was. However, in consideration of efficiency, it was more convenient to use ethanol aqueous solution as a solvent. Therefore, it is preferable to extract with 70% (v / v) ethanol. Here, 70% (v / v) hydrous ethanol means ethanol having 70% (v / v) of ethanol and 30% (v / v) of water.

The cosmetic composition of the present invention may be added to the cosmetic amount of 0.1% to 10% by weight of the dandelion, self-leaving, participating fern, lotus root, scented and loquat leaf mixed extract as a liquid weight.

The addition method is added to the cosmetic in an amount of 0.001 to 5% by weight, preferably 0.01 to 3% by weight as a dry weight thereof while recovering the solvent evaporated using a distillation apparatus equipped with a cooling condenser. If it is less than 0.001% by weight, its efficacy is insignificant, and if it is more than 5% by weight, there is no significant difference. Therefore, the cosmetic composition of the present invention preferably contains a mixed extract in an amount of 0.001 to 5% by weight based on the total dry weight of the cosmetic composition.

The formulation of the cosmetic composition of the present invention may be in various forms. For example, it may be softening cream, nutrient cream, nourishing cream, massage cream, essence or pack. That is, the present invention, in the antimicrobial and atopic skin improvement cosmetic composition of each formulation, other components in addition to the dandelion and cotyledon extract can be arbitrarily selected and blended according to the formulation or purpose of use of other cosmetics. Other ingredients used in each formulation are already available to those skilled in the art for purified water, oils, surfactants, humectants, higher alcohols, chelating agents, pigments, fatty acids, antioxidants, preservatives, waxes, pH adjusters, Spices and the like.

The cosmetic composition of the present invention is preferably for antioxidant and antibacterial or atopic skin improvement.

In addition, the present invention extracts, concentrated and mixed with dandelion, cotyledon, fern, lotus root, scented and loquat leaf with a solvent selected from the group consisting of water, anhydrous or hydrous alcohol of 1 to 4 carbon atoms, and ethyl acetate It provides a method for producing a mixed extract cosmetic composition comprising the step of preparing an extract. Preferred extraction solvents are 70% (v / v) ethanol.

In the production method of the present invention, the extraction is preferably made for 3 hours to 24 hours at 50 ℃ to 100 ℃ or 3 to 20 days at 4 ℃ to 30 ℃.

In addition, the production method of the present invention may further comprise the step of removing the diethyl ether soluble component before or after performing the extraction process.

The cosmetic composition of the present invention is excellent in cell proliferation and non-toxic effect, antioxidant effect, antibacterial effect on various bacteria and atopic skin improvement effect, it can be used as functional cosmetic composition for antioxidant, antibacterial and atopic skin improvement.

Hereinafter, preferred embodiments of the present invention will be described in order to facilitate understanding of the present invention. However, the following Examples and Experimental Examples are provided only to more easily understand the present invention, and the contents of the present invention are not limited thereto.

In addition, dandelion, self-leaving, participating fern, lotus root, scented and loquat leaf mentioned below were purchased from Gyeongdong Pharmaceutical Market in Korea, and the extraction solvent was purchased from DAICEL CHEMICAL INDUSTRIES, LTD.

Example 1 Preparation of a Dandelion, Pleurotus eryngii, Participating Ferns, Lotus Root, Night Scent, and Loquat Leaf Extract

100 g of dried dandelion, self-leaving, fern, lotus root, scented and loquat leaf, respectively, was pulverized into 10 to 200 mesh size using a herbal medicine grinder, and then ultrasonic wave was added by 500 ml of 70% (v / v) ethanol aqueous solution. After the ultrasonic wave was added to the vibration and the active ingredient including the inside of the cell membrane was extracted in ethanol, the mixture was cooled for 24 hours at room temperature, and then filtered using 0.40 μm filter paper. The filtered extracts were concentrated under reduced pressure at 45 ° C. to obtain respective concentrates.

For each of the extracts obtained above, the minimum inhibitory concentration test (Minimum inhibitory Concentration, MIC, reference: Food Microbiology Laboratory, Geocultural History, 2000) of Escherichia coli (Buyer: BRC strain No. KTCT 2571, ATCC8739) Therefore, the optimum active concentration was confirmed at the same concentration of each extract, the results are shown in Table 1.

Extract ingredients Escherichia coli 0.25% 0.5% One% 2% dandelion + + - - Lobule + + - - Exhibitor + + + - Lotus root + + + - Scent + - - - Loquat leaf + + + -

In the above table, the medium is opaque due to the absence of antimicrobial activity, and the medium is transparent due to the antibacterial activity.

Based on the results shown in Table 1, 1% by weight, 1% by weight, 2% by weight, 2% by weight, 2% by weight, 0.5% by weight and 2% by weight of Dandelion, Prunus leaf, Participatum, lotus root, Scent and Loquat leaf extract, respectively, A mixed extract containing the remaining water was prepared.

Comparative Example 1. Dandelion Extract Preparation

After crushing the dried dandelion 100g into 10 ~ 200 mesh size with a herbal mill, 500ml of 70% (v / v) ethanol aqueous solution was added to the ultrasonic vibration and ultrasonic vibration was applied to extract the active ingredient including the cell membrane in ethanol After cooling for 24 hours at room temperature, sterilization was filtered using 0.40㎛ filter paper. The filtered extract was concentrated under reduced pressure at 45 ° C. to obtain the title extract.

Comparative Example 2. Preparation of Fructus Leaf Extract

After grinding 100 g of dried leaflets to 10-200 mesh size with a herbal medicine grinder, 500 ml of 70% (v / v) ethanol aqueous solution was added to ultrasonic vibration and ultrasonic vibration was performed to extract the active ingredients including the cell membranes into ethanol. After cooling at room temperature for 24 hours, sterilization was filtered using 0.40 μm filter paper. The filtered extract was concentrated under reduced pressure at 45 ° C. to obtain the title extract.

Comparative Example 3. Participating fern extract preparation

100 g of the dried entry fern was pulverized into 10 to 200 mesh size using a herbal medicine grinder, and 500 ml of 70% (v / v) ethanol aqueous solution was added to ultrasonic vibration, and ultrasonic vibration was applied to extract the active ingredient including the cell membrane in ethanol. After cooling at room temperature for 24 hours, sterilization was filtered using 0.40 μm filter paper. The filtered extract was concentrated under reduced pressure at 45 ° C. to obtain the title extract.

Example 2. Makeup

The lotion (skin lotion) of the cosmetic composition containing the dandelion, the self-leaving, participating fern, lotus root, scented and loquat leaf extract of Example 1 was prepared in the composition of Table 2 below.

number Raw material Content (% by weight) One Dandelion, jasminoides, fern, lotus root, scented and loquat leaf extract (Example 1) 1.0 2 glycerin 3.0 3 Butylene Glycol 2.0 4 Propylene glycol 2.0 5 Polyoxyethylene Cured Castor Oil 1.0 6 ethanol 10.0 7 Triethanolamine 0.1 8 antiseptic Quantity 9 Pigment Quantity 10 Spices Quantity 11 Purified water Balance

To No. 11 shown in Table 2, No. 2, 3, 4, and 8 were sequentially added and stirred to dissolve. 5 times was melt | dissolved in this solution about 60 degreeC, and 10 times was added, and it melt | dissolved in 11 times. Finally, 1,6,7,9 was added to this solution, and the mixture was sufficiently stirred and aged at room temperature for 72 hours to prepare a lotion according to the present invention.

Example 3. Nutritional Lotion Preparation

Nutritional lotion was prepared in the composition of Example 1 containing dandelion, self-leaving, fern, lotus root, scented and loquat leaf extract of Example 1 in the composition of Table 3.

number Raw material Content (% by weight) One Dandelion, jasminoides, fern, lotus root, scented and loquat leaf extract (Example 1) 1.0 2 Beeswax 1.0 3 Polysorbate 60 1.5 4 Solbitan Sesquioleate 0.5 5 Floating paraffin 10.0 6 Sorbitan stearate 1.0 7 Lipophilic Monostearic Acid Glycerin 0.5 8 Stearic acid 1.5 9 Glyceryl Stearate / Pig-400 Stearate 1.0 10 Propylene glycol 3.0 11 Carboxy Polymer 0.1 12 Triethanolamine 0.2 13 antiseptic Quantity 14 Pigment Quantity 15 Spices Quantity 16 Purified water Balance

10, 11, 13, and 16 described in Table 3 were mixed and stirred at 80 to 85 ° C., and put into the manufacturing unit, followed by actuating an emulsifier. 2, 3, 4, 5, 6, 7, 8, 9, 12 was dissolved after heating to 80 ~ 85 ℃. After emulsification, the mixture was cooled to 50 ° C. while stirring using a stirrer, and 15 times were added thereto, followed by cooling to 45 ° C. and 14 times. Then, 1 time at 35 ℃ was cooled to 25 ℃ and aged for 72 hours to prepare a nutrition lotion according to the present invention.

Example 4 Preparation of Nutritional Cream

Nutritional cream in the cosmetic composition containing a dandelion, jasminoid leaf, fern, lotus root, scented and loquat leaf extract of Example 1 was prepared in the composition shown in Table 4.

number Raw material Content (% by weight) One Dandelion, jasminoides, fern, lotus root, scented and loquat leaf extract (Example 1) 1.0 2 Stearic acid 2.0 3 Cetyl alcohol 2.0 4 Glyceryl Monostearate 2.0 5 Polyoxyethylene sorbitan monostearate 0.5 6 Sorbitan sesquioleate 0.5 7 Glyceryl Monostearate / Glyceryl Stearate / Polyoxyethylene Stearate 1.0 8 Wax 1.0 9 Liquid paraffin 4.0 10 Squalane 4.0 11 Caprylic / capric triglyceride 4.0 12 Carboxy Vinyl Polymer 0.3 13 Butylene glycol 5.0 14 glycerin 3.0 15 Triethanolamine 0.5 16 Purified water Balance

12, 13, 14 and 16 shown in Table 4, the mixture was stirred and heated to 80 ~ 85 ℃ while stirring the emulsifier 2, 3, 4, 5, 6, 7, 8, 9, 10 and 11 are heated to 80 ~ 85 ° C to dissolve and 15 times is added and stirred to put into the manufacturing unit and emulsified. After emulsification, the mixture was cooled to 35 ° C. while stirring using a stirrer, 1 time was added, cooled to 25 ° C., and aged for 72 hours to obtain a nourishing cream.

Example 5. Essence Preparation

Essence was prepared in the composition of Table 5 containing a dandelion, jasminoid leaf, fern, lotus root, scented and loquat leaf extract of Example 1 in Table 5.

number Raw material Content (% by weight) One Dandelion, jasminoides, fern, lotus root, scented and loquat leaf extract (Example 1) 1.0 2 Cytostolol 1.7 3 Polyglyceryl 2-oleate 1.5 4 Ceramide 0.7 5 Steares-4 1.2 6 cholesterol 1.5 7 Dicetylphosphate 0.4 8 Concentrated glycerin 5.0 9 Macadamia oil 15.0 10 Carboxy Vinyl Polymer 0.2 11 Xanthan Gum 0.2 12 antiseptic Quantity 13 Spices Quantity 14 Purified water Balance

Nonionic amphiphilic lipids were prepared by homogenizing Nos. 2, 3, 4, 5 and 6 described in Table 5 at a constant temperature. The nonionic amphiphilic lipids were mixed with Nos. 1, 7, 8 and 14, homogenized at a constant temperature, passed through a microfluidizer, and then added with No. 9 at a constant temperature, homogenized, and then again added to the microfluidizer. I passed it again. Thereafter, 10, 11, 12, and 13 were added to disperse and stabilize, and aged at room temperature for 72 hours to prepare an essence according to the present invention.

Comparative Examples 4 to 6: Preparation of nutrition cream

Nutritional creams corresponding to Comparative Examples 4 to 6 were prepared in the same manner as in Example 4, except that Comparative Example 1 or 2 was added instead of the extract of Example 1 in Example 4.

number Raw material Comparative Example 4 Comparative Example 5 Comparative Example 6 2 Stearic acid 2.0 2.0 2.0 3 Cetyl alcohol 2.0 2.0 2.0 4 Glyceryl Monostearate 2.0 2.0 2.0 5 Polyoxyethylene sorbitan monostearate 0.5 0.5 0.5 6 Sorbitan sesquioleate 0.5 0.5 0.5 7 Glyceryl Monostearate / Glyceryl Stearate / Polyoxyethylene Stearate 1.0 1.0 1.0 8 Wax 1.0 1.0 1.0 9 Liquid paraffin 4.0 4.0 4.0 10 Squalane 4.0 4.0 4.0 11 Caprylic / capric triglyceride 4.0 4.0 4.0 12 Carboxy Vinyl Polymer 0.3 0.3 0.3 13 Butylene glycol 5.0 5.0 5.0 14 glycerin 3.0 3.0 3.0 15 Triethanolamine 0.5 0.5 0.5 16 Purified water Balance Balance Balance 17 water 1.0 - - One Comparative Example 1 - 1.0 - One Comparative Example 2 - - 1.0

Experimental Example 1 Confirmation of Cell Nontoxicity

For the extracts prepared in Example 1 and Comparative Examples 1 to 3 was measured the cell proliferation effect using cell culture. Cell proliferation and toxicity tests were performed according to the methods described in the following literature (Mosmann, T., Rapid Colorimetric Assay for Cellular Growth and Survival: Application to Proliferation and Cytotoxicity Assays. J. Immunol. Meth. 1983, 65, 55-63 .). Medium used for cell proliferation and toxicity test was human fibroblast cell line (ATCC, CRL-) in Dulbeco's Modified Eagle's Medium (DMEM) containing 10% fetal bovine serum (FBS). 2076), Purchasing: Bank of Korea Cell Line] was seeded on a 24 well plate at a concentration of 1 × 10 ⁵ cells / ml. After 24 hours of seeding, the medium was replaced, and the extract of Example 1 and the extracts of Comparative Examples 1 to 3 were added by concentration, and then cultured in 37 ° C. and 5% CO ₂ incubator for 3 days. After adding 0TT (Tetrazolium-based colorimetric) solution 1ml / well each, after 4 hours at 37 ℃, CO ₂ incubator, the media was removed and 200μL of DMSO (dimethylsulfoxide) was added thereto and the absorbance was measured at 550nm. The results are shown in Table 7. The control is untreated sample.

Extract concentration
(%) Cell proliferation rate (%) Comparative Example 1 Comparative Example 2 Comparative Example 3 Example 1 Control 100 100 100 100 0.01 100.21 100.18 100.93 100.93 0.05 100.59 101.21 101.76 101.76 0.1 113.21 115.70 116.12 116.12 0.3 116.62 118.43 125.56 138.56 0.5 125.32 138.32 144.62 142.62 1.0 129.43 142.95 148.53 165.53

As shown in Table 7, the mixed extract Example 1 does not exhibit toxicity at the cellular level, and thus can be applied as a safe cosmetic composition to humans, and has an effect of proliferating cells better than Comparative Examples 1 to 3, which are single extracts. It can be seen that.

Experimental Example 2 Confirmation of Antioxidant Effect

For Comparative Examples 1 to 3 and Example 1 using the DPPH (2,2-Diphenyl-1-picrylhydrazyl) by measuring the free radical scavenging rate (Free Radical) was confirmed the antioxidant effect. 0.3 ml of Comparative Examples 1-3 and Example 1 were added to 1 ml of 0.2 mM DPPH methanol solution, respectively, and the mixture was stirred well at room temperature and allowed to react for 10 minutes. The absorbance was measured at 520 nm, and the value of free radical 50% scavenging concentration (IC ₅₀ ) was calculated and the results are shown in Table 8 below.

IC ₅₀ (Sample%) Comparative Example 1 2.4 Comparative Example 2 2.2 Comparative Example 3 2.4 Example 1 1.5

As shown in Table 8, it is confirmed that the free radical 50% scavenging concentration (IC ₅₀ ) of Example 1 has a value about 2 times improved compared to Comparative Examples 1 to 3 which is a single extract. Therefore, Example 1 has superior antioxidant efficacy compared to Comparative Examples 1 to 3.

Experimental Example 3 Confirmation of Inhibitory Effect of NK-kB Activity

Inflammation of the inflammatory response in immune cells is induced by the LPS inflammation is known to increase the gene expression of IL-8 and TNF-α as the NF-kB activity known as inflammatory cytokines increases. Thus, for Example 1 and Comparative Examples 1 to 3 were measured the inhibitory effect of NF-kB activity reported to play an important role in the production of basophilic cytokines in this inflammatory response system. After dispensing 1x10 ⁶ cells into 6 wells of THP-1 cells (purchased from Korea Cell Line Bank, TIB-202), which are human monocytes, NF- was purified using a superperfect transfection reagent. kB luciferase reporter plasmid DNA (NF-kB luciferase reporter plasmid DNA, Stratagene, purchased) was transfected. After 24 hours after transfection, lipopolysaccharide (LPS, purchased from Sigma) (100 ng / ml) was treated to activate THP-1 cells and at the same time extracts of Examples 1 and 3 Was treated by concentration (1, 5, 10, 20, 30 ppm). After 24 hours, cells were collected and NF-kB activity was measured using a luminometer, and the control group was untreated. The inhibition (%) of the measured NF-kB activity inhibition effect was calculated by the following Equation 1, and the results are shown in Table 9.

[Equation 1]

inhibition (%) = [(A-B) / A] X100

(A: Control value B: Sample treatment value)

density
(ppm) Inhibition (%) Comparative Example 1 Comparative Example 2 Comparative Example 3 Example 1 One 4.05 4.01 4.41 5.01 5 15.74 15.23 15.45 18.71 10 32.41 39.24 40.21 42.45 20 50.53 76.41 77.67 89.51 30 78.54 87.45 85.42 97.54

As shown in Table 9, the composition of the present invention has a superior NF-kB activity inhibitory effect than a single extract.

Experimental Example 4 Confirmation of Antimicrobial Effects

The extract of Example 1 and Comparative Examples 1 to 3 was used as a sample of the antimicrobial activity of 0.1 wt%. Each strain was inoculated with 100 ml of liquid medium and incubated at 32 ° C for 20 hours to activate. 100 μl of the culture solution was inoculated into each medium (10 ⁷ to 10 ⁸ cfu / ml), and a plate of paper plate (6 mm in diameter) in which 20 μl of the sample was prepared and plated for each strain was placed in the center of the medium for 36 hours. After incubation, the diameter of the resulting inhibitory ring was measured. The control group was diluted solvent DW, and strains were selected from Staphylocuccus aureus and Candida albicans . The growth conditions of the bacteria were carried out as shown in Table 10, and the results of the antibacterial activity test by inhibition ring production measurement are shown in Table 11.

Strain badge Incubation time, temperature Staphylocuccus aureus (ATCC 29737) Nutrient agar 48 hours, 37 ° C Candida albicans (ATCC 10259) Nutrient agar 48 hours, 37 ° C

sample Diameter of Inhibitory Ring (mm) Staphylocuccus aureus Candida albicans Comparative Example 1 10 11 Comparative Example 2 10 10 Comparative Example 3 10 10 Example 1 12 13

As shown in Table 11, the extract of the present invention was confirmed to have excellent antibacterial activity against a variety of staphylococcus aureus, fungi compared to the extract of dandelion, cotyledon and fern alone.

Experimental Example 5 Lipoxygense Activity Inhibition Effect

Among the anti-inflammatory effects, it is an experiment to confirm the inhibitory effect of lipoxygenase activity related to the induction of inflammation. Lipoxygenase was used as a product from sigma purified from soybean and diluted to 563 unit / ml. 5 μl and 20 ul of the enzyme solution of Example 1 or Comparative Examples 1 to 2 were added to 155 ul of Tris-HCl (100 mM, pH 8.5) buffer in a UV passage 96 well plate and allowed to stand at room temperature for 5 minutes. Then, 20 μl of 33 μM linolenic acid (sigma, USA) was added and the change in absorbance was measured for 200 seconds at 234 nm. The lipoxygenase inhibitory activity of Comparative Example 1, Comparative Example 2, and Example 1 with the change in absorbance was calculated by the following Equation 2, which is shown in Table 12. The control is untreated sample.

[Equation 2]

inhibition (%) = [(A-B) / A] X100

(A: control absorbance measured value B: sample treated absorbance measured value)

Example 1 showed a better and more effective result of lipoxygenase activity inhibitory effect than the single extract Comparative Examples 1 and 2.

Experimental Example 6 Determination of Degranulation Inhibition Effect

In order to confirm the degranulation of immune cells by measuring the inhibitory effect of β-hexosaminidase secretion through cell experiments, a test was conducted to evaluate the effects of inflammation and allergic reaction. RBL-2H3 cell line was obtained from Eagle's medium (Dulbeccos' modified Eagle's medium, DMEM) containing 1% Fetal bovine serum and L-glutamine. The cells were cultured in a 37 ° C. 5% CO ₂ incubator, and the cells with adherence were suspended using trypsin-EDTA solution, and then separated and recovered. After activating the RBL-2H3 cell line with activator (Dinitrophenol-conjugated human serum albumin, (DNP-HSA) -specific IgE), the activated cells were treated with 50 ng / mL of antigen (DNP-HSA). The extracts of Example 2 and Example 1 were treated at various concentrations and incubated for 60 minutes (FIG. 1A) and 120 minutes (FIG. 1B). Thereafter, the amount of β-hexosaminidase secreted into the medium was measured using an ELISA reader, and inhibition (%) was calculated through the following Equation 3, shown in Table 13 below. The control is the sample untreated.

[Equation 3]

inhibition (%) = [(A-B) / A] X100

(A: Control value B: Sample treatment value)

In Example 1, the amount of β-hexosaminidase was decreased with increasing concentration compared to Comparative Examples 1 and 2, and the inhibitory effect was more effective in Example 1 than Comparative Examples 1 and 2.

Experimental Example 7 Inhibition of Lipid Peroxidation Effect

The inhibitory effect of Example 1, Comparative Example 1 and Comparative Example 2 on lipid peroxidation that may be the cause of atopic dermatitis was confirmed. After dissolving linolenic acid in 0.1% aqueous solution of 8% sodium lauryl sulfate to 3.9 ml, 0.1 ml of Comparative Example 1, Comparative Example 2 and Example 1 were added thereto, followed by ultraviolet irradiation for 1 hour. After taking 1 ml of this solution, 1.5 ml of 0.8% TBA aqueous solution and 1.5 ml of 20% acetic acid (pH 3.5) were added, and the mixture was cooled for 1 hour by cooling, followed by 1 ml of purified water and 5 ml of n-BuOH: Pyridine (15: 1). And shaken. After centrifugation, the n-BuOH layer was taken and absorbance was measured at 532 nm. The inhibition of lipid peroxidation [inhibition (%)] was calculated using Equation 4 below, and the results are shown in Table 14. The control is untreated sample.

[Equation 4]

inhibition (%) = [(A-B) / A] X100

(A: Control value B: Sample treatment value)

As can be seen in Table 14, it was confirmed that Example 1 has a superior lipid peroxidation inhibitory effect at each concentration compared to Comparative Examples 1 and 2.

<Experiment 8> Confirm the whitening effect

The whitening effect that can improve skin pigmentation due to atopy was measured for Examples 1, Comparative Examples 1 and 2 in the following manner.

murine melanoma (B16 F10) cells (purchased from Korea Cell Bank) were inoculated at 1 × 10 ⁵ per well in 6-well plates with DMEM medium containing 10% FBS (fetal bovine serum), followed by 5% CO ₂ and Cells were incubated at 37 ° C. until at least about 80% adhered to the bottom of the well. After incubation, the medium was removed, and Example 1, Comparative Examples 1 and 2 were replaced with a medium diluted to 0.5% by weight without cytotoxicity, and then cultured under 5% CO ₂ and 37 ° C for 3 days. The cells from which the medium was removed were washed with phosphated buffer saline (PBS) and treated with trypsin to recover the cells. The recovered cells were measured by using a hematocytometer, and then centrifuged at 5,000 to 10,000 rpm for 10 minutes, and then the supernatant was removed to obtain pellets. After drying the cell pellet at 60 ° C, 100 µl of 1M sodium hydroxide solution containing 10% DMSO was added to obtain intracellular melanin in a 60 ° C thermostat. Using this solution, absorbance at 490 nm was measured with a microplate reader, and the amount of melanin per cell was determined. The value was calculated by using Equation 5 below and shown in Table 15 below. The control is untreated sample.

[Equation 5]

inhibition (%) = [(A-B) / A] X100

(A: Control value B: Sample treatment value)

sample Melanin production inhibition rate (%) Comparative Example 1 50 Comparative Example 2 43 Example 1 68

As shown in Table 15, Example 1 according to the present invention was superior to the melanin inhibition rate compared to Comparative Examples 1 and 2.

<Experiment 9> Confirmation of stratum corneum moisture retention recovery

Skin stratum corneum moisture retention recovery experiments were performed for Example 4 and Comparative Examples 4 to 6. Twenty male and female subjects aged 20 to 40 years were treated with 5% sodium dodecyl sulfate and then rested in a thermo-hygrostat room for more than 30 minutes. In addition, after setting the compartment of 4X4 ㎠ inside the lower arm, the sample was applied 3 to several times a day with a thickness of 2 ㎍ / ㎠ and the stratum corneum moisture evaporation (TEWL) measuring device (Tewameter, Courage + Khazake electronic over time) Gmbh, Germany) was used to measure the moisture content of the stratum corneum, and the untreated group without SDS treatment was also measured in order to compare the self-healing recovery. The results were shown in Table 16.

From the results in Table 16, it can be seen that Example 4, which is a cosmetic of the present invention, exhibits an excellent recovery effect after skin barrier damage than cosmetics of a single extract.

<Experiment 10> Confirmation of atopic skin improvement effect 1

Skin improvement effect was measured in 30 adults with atopic dermatitis with respect to the cosmetics prepared in Example 4 and Comparative Example 5.

The test subjects were randomly divided into two sets, and each sample was applied to the whole body by dividing each of Example 4 and Comparative Example 5 into the right and left sides by a double-blinded test, but it could affect the effect of the test sample as much as possible. Use of other moisturizers is prohibited. The effects after 1, 2, 3 and 4 weeks after application of the test sample were evaluated by SCORAD (SCORAD: SCORing Atopic Dermatitis) measurement and the results are shown in Table 17 below. Also shown in FIG. 1 is a photograph of the clinical results of any one subject before and after the use of cosmetics containing dandelion, foliage, fern, lotus root, scented and loquat leaf extract.

1) Criteria

① Extent criteria: Area = damage area / 100

② intensity criteria

-Erythema (1/2/3),-edema (1/2/3),-exudation (1/2/3),-peeling skin (1/2/3),

-Degree of limbness (1/2/3),-degree of drying (1/2/3)

Subjective criteria

-Itching (1-10),-insomnia (1-10)

* Score calculation = (accuracy criterion / 5) + (strength criterion / 7) + subjective criterion

Referring to Table 17, it can be seen that the cosmetic of Example 4 has a very good atopic skin improvement effect compared to the cosmetic of Comparative Example 5. In particular, the results after 4 weeks of Example 4 shows an excellent improvement of about 84%.

Experimental Example 10 Confirmation of Atopic Skin Improvement Effect 2

In order to examine the atopic dermatitis alleviating effect that the caregiver feels, after applying the cosmetics of Example 4 and Comparative Example 5 for one month to 20 children with atopy, the parents of the user according to the following criteria, itching, erythema change, It was shown in Table 18 to evaluate the degree of improvement of the dermatitis and the side effects caused by the test sample, along with the satisfaction with the dry skin.

(Criteria)

One 2 3 4 5 no effect Some usually Great Very good Very dissatisfied dissatisfaction usually satisfied very good

Classification Itching of the skin Erythema change Dry skin Overall improvement Side Effect Example 4 4.21 3.82 3.92 4.52 4.09 Comparative Example 5 2.25 3.17 3.14 3.11 3.95

As shown in Table 18, the cosmetic of Example 4 according to the present invention can be confirmed that there is an improvement effect in all the evaluation items for children with atopic skin as a test subject.

1 is a clinical picture before and after the use of the cosmetic composition of the present invention according to Experimental Example 10.

Claims (11)

A cosmetic composition comprising a mixed extract of Taraxacum officinlale, Perilla ocymoides, Particle (Gloiopeltis tenax), Neilbo Nucifera Seed, Cestrum nocturnum, and Eriobotrya Japonica Leaf. The method of claim 1, wherein the extract is dandelion extract: cotyledon extract: fern extract: lotus root extract: scent extract: loquat leaf extract 1: 1: 2: 2: 0.5: 2 is a cosmetic composition is mixed in a weight ratio Composition. The cosmetic composition of claim 1, wherein the mixed extract is 70% (v / v) ethanol extracted from dandelion, cotyledon, fern, lotus root, scented fragrance, and loquat leaf. The cosmetic composition according to claim 1, wherein the mixed extract is contained in an amount of 0.001 to 5% by weight based on the total dry weight of the cosmetic composition. The cosmetic composition according to claim 1, wherein the cosmetic composition is in the form of softening cream, nutrient cream, nourishing cream, massage cream, essence or pack. According to claim 1, wherein the cosmetic composition is a cosmetic composition for antioxidant and antibacterial. According to claim 1, wherein the cosmetic composition is a cosmetic composition for improving atopic skin Taraxacum officinlale, Perilla ocymoides, Gloiopeltis tenax, Neilbo Nucifera Seed, Cestrum nocturnum, and Eriobotrya Japonica Leaf with 1 to 4 carbon atoms Extracting with a solvent selected from the group consisting of anhydrous or hydrous alcohol, and ethyl acetate, and then concentrated to produce a mixed extract. The method of claim 8, wherein the extraction is performed at 50 ° C to 100 ° C for 3 hours to 24 hours. The method of claim 8, wherein the extraction is performed for 3 to 20 days at 4 ℃ to 30 ℃. The method according to any one of claims 8 to 10, wherein the solvent is 70% (v / v) hydrous ethanol.

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