KR102540377B1 - Manufacturing method of antimicrobial composition containing natural plant extract and antimicrobial composition thereof - Google Patents

Abstract

The present invention relates to a method for preparing an antibacterial composition containing a plant extract as an active ingredient and to a plant extract antibacterial composition prepared thereby, comprising a plant extract containing a cypress tree extract, a moss extract, and a Pogongyeongyeong extract as an active ingredient, and an antibacterial composition By manufacturing phytoncide, it has excellent antibacterial and deodorizing properties, which are unique to phytoncide, and helps to improve health with antioxidant, antibacterial, anti-inflammatory, anti-stress, and various physiological activities. To provide a method for preparing an antibacterial composition containing as an active ingredient a plant extract capable of removing insects and enhancing a sense of comfort and soothing due to phytoncide, and a plant-extracted antibacterial composition prepared thereby, Obtaining a cypress extract, a moss extract, and a Pogongyoung extract from moss plants and Pogongyoung; Vacuum low-temperature extraction of the obtained cypress extract, moss extract, and Pogongyoung extract, respectively; Cooling the vacuum low-temperature extracted cypress extract, moss extract, and Porconia extract; Distilling the cooled cypress extract, moss extract, and Porconia extract; and adding water or ethanol to the mixing tank and then adding and mixing the distilled cypress extract, moss extract, and Pogongyoung extract; It is characterized in that it includes.

Description

Manufacturing method of antimicrobial composition containing plant extract as an active ingredient and plant extract antimicrobial composition prepared thereby {Manufacturing method of antimicrobial composition containing natural plant extract and antimicrobial composition thereof}

The present invention relates to a method for preparing an antibacterial composition containing a plant extract as an active ingredient and to a plant-derived antibacterial composition prepared thereby, and more particularly, to a plant extract including a cypress tree extract, a moss extract, and a Pogongyeongyeong extract as an active ingredient. It increases the body's immune function, helps improve health with antioxidant, anti-inflammatory, anti-cancer, anti-stress and various physiological activities, provides deodorization, deodorization and sterilization effects with antibacterial action, and provides a pleasant feeling and soothing sensation due to phytoncide. It relates to a method for preparing an antibacterial composition comprising a plant extract capable of enhancing the sense of smell as an active ingredient, and to a plant-extracted antibacterial composition prepared thereby.

In recent years, fine dust and the like have emerged as a social problem in an environment in which air pollution is getting worse and yellow dust is frequently generated.

Fine dust is divided into fine dust with a particle size of less than 10 μm and ultra-fine dust with a particle size of less than 25 μm.

Since these fine dust particles are very small in size, they can stay in the air and penetrate the lungs through the human respiratory tract or move into the body along the blood vessels, thereby adversely affecting health.

On the other hand, when microorganisms or bacteria ingest and decompose contaminants such as sweat or sebum secreted from the body, inflammation is induced by strong skin irritation, and bacteria such as Staphylococcus and Escherichia coli may cause a strong odor.

In particular, because certain parts of the body have favorable humidity and temperature conditions for bacteria to propagate, various microorganisms such as bacteria, fungi, pathogenic bacteria, athlete's foot bacteria, and fungi can proliferate, resulting in skin irritation, itching, Inflammation, blisters, rashes, and malodor may be produced.

In addition, when various microorganisms proliferate vigorously on used clothing, bedding, and shoes, severe odors may occur, and environments in which a large number of people live together in groups, such as military bases, dormitories, and training centers. In the environment, the frequency of exposure to various microorganisms harmful to the body increases, causing infection and odor.

Various antibacterial agents have been developed to purify fine dust as described above and inhibit the propagation and proliferation of various microorganisms. BACKGROUND ART Chemical detergents for skin diseases, chemicals for skin diseases to eradicate bacteria that live in the human body and provide the cause of inflammation have been developed and used.

However, since antibacterial agents for different purposes are required depending on the type of microorganisms, antibacterial agents are required according to the place or object to be removed, and antibacterial agents harmless to the human body must be provided, it is not easy to distinguish each antibacterial agent. However, there is a problem that the user's cost burden is increased.

In order to solve the above-mentioned problems, a technology for preparing cypress tree as a liquid composition and obtaining antibacterial, insect repellent, aromatic, and deodorizing effects through active ingredient phytoncide is disclosed in Korean Patent Publication No. 10-2012-0025740, A technology applied as a deodorant, fragrance, etc. in the form of a composition mixed with other natural oil components is disclosed in Korean Patent Registration No. 10-1439385 and Korean Patent Registration No. 10-1161415.

Phytoncide, an active ingredient of cypress trees, is a substance with antibacterial, insect repellent, aromatic, and deodorizing effects.

Here, phytoncide is a compound word of phyton, which means plant, and cide, which means bactericidal power. and has an excellent deodorizing effect, so phytoncide made from cypress trees as a liquid composition is sprayed and used.

Although these known technologies contain the active ingredients of cypress trees, there is no disclosure of a technology for enhancing the effect of adding a moss extract and a Pogongyoung extract to a phytoncide solution.

Accordingly, there is a demand for development of a general-purpose antibacterial agent that can be widely used for various purposes and can be used more effectively.

Republic of Korea Patent No. 10-1711050 Republic of Korea Patent No. 10-1698740

The present invention is intended to solve the above problems, and by preparing an antibacterial composition using plant extracts including cypress tree extract, moss extract and Pogongyeongyeong extract as active ingredients, excellent antibacterial and deodorizing properties, which are unique characteristics of phytoncide Antioxidant, antibacterial, anti-inflammatory, anti-stress and various physiological activities help to improve health, antibacterial action deodorizes, deodorizes, sterilizes and removes insects such as mites, and phytoncide provides a sense of comfort and soothing It is an object of the present invention to provide a method for preparing an antibacterial composition comprising a plant extract as an active ingredient and a plant extract antibacterial composition prepared thereby.

In addition, the present invention is harmless to humans and animals and can be widely used for treating various skin diseases and atopy that can be caused by various germs, bacteria and harmful pathogens, and can be used to combat pests such as ticks or mosquitoes, , Can inhibit the proliferation of various bacteria and harmful pathogens, and public places such as schools, government offices, churches, rest areas, waiting rooms, senior citizens' homes, hospitals, offices, restaurants, buses, trains, karaoke rooms, homes or toilets where many people gather It is an object of the present invention to provide a method for preparing an antibacterial composition comprising, as an active ingredient, a plant extract capable of preventing the propagation and transmission of various bacteria and harmful pathogens, and a plant extract antibacterial composition prepared thereby.

In addition, the technical problems to be achieved by the present invention are not limited to the above-mentioned technical problems, and other technical problems not mentioned are clearly understood by those skilled in the art from the description below. It could be.

In order to achieve the above object, the present invention provides a step of obtaining a cypress tree extract, a moss extract, and a Pogongyoung extract from a cypress tree, a moss plant, and Pogongyoung; Vacuum low-temperature extraction of the obtained cypress extract, moss extract, and Pogongyoung extract, respectively; Cooling the vacuum low-temperature extracted cypress extract, moss extract, and Porconia extract; Distilling the cooled cypress extract, moss extract, and Porconia extract; and adding water or ethanol to the mixing tank and then adding and mixing the distilled cypress extract, moss extract, and Pogongyoung extract; It is characterized in that it includes.

Here, extracting the obtained cypress tree extract, moss extract, and pogongyeongyeong extract with water or alcohol, respectively, at a weight ratio of 1: 1 to 20 in a vacuum at low temperature; more includes

Preferably, 5% to 30% by weight of the cypress extract obtained in 50% to 85% by weight of water or ethanol, and 3% to 10% by weight of the obtained moss extract, relative to the total 100% by weight in the mixing tank , and 1% by weight to 10% by weight of the obtained Pogongyoung extract were added and mixed.

Here, when adding water or ethanol, cypress tree extract, moss extract, and Pogongyoung extract to the mixing tank, additional materials are added, but the supplementary material is water-soluble silicate.

In addition, when adding water or ethanol, cypress extract, moss extract, Pogongyeong extract, and auxiliary materials to the mixing tank, additives are further added, and at least one of jasmine powder, peppermint powder, or pine needle powder is further added.

As described above, the present invention having the above configuration, by preparing an antibacterial composition using a plant extract including a cypress tree extract, a moss extract, and a Pogongyeong extract as an active ingredient, anti-oxidation, anti-inflammatory, anti-cancer, anti-inflammatory, anti-stress and It helps improve health with various physiologically active functions, increases immunity by being absorbed into the human body, promotes blood circulation, psychological stability, sterilization and physiological activity, and improves comfort and soothing sensation due to phytoncide, and at the same time improves the five senses. It has the effect of enhancing emotional stability by providing satisfaction.

In addition, the present invention, made of plant extracts, is harmless to humans and animals, and has excellent antibacterial and deodorizing properties inherent in phytoncide, so that it can sterilize and remove pests such as mites and mosquitoes, remove odors and odors, sterilize, It has excellent effects and efficacy on insecticide and antibacterial, can neutralize formaldehyde, has antibacterial effect not only on general bacteria, but also has excellent antibacterial effect on antibiotic-resistant bacteria (super bacteria), and not only decomposes odors and odors. In addition, it removes the cause of odor and decomposes the cause of odor at the same time to fundamentally remove and deodorize food odor, toilet odor, air conditioner odor, and cigarette odor, thereby obtaining an air purification effect and proliferating various germs, harmful pathogens and bacteria. It can be widely used for various skin diseases including atopy by suppressing, can sterilize E. coli, Staphylococcus aureus, Pseudomonas aeruginosa and Salmonella harmful to the human body, and is effective for sick house syndrome and sick car syndrome.

In addition, the present invention can inhibit the growth of various germs, harmful pathogens and bacteria, so schools, government offices, churches, rest areas, waiting rooms, senior citizens' homes, hospitals, offices, restaurants, buses, trains, karaoke rooms, homes where many people gather It is used in public places such as bathrooms and has the effect of preventing the propagation and transmission of various germs and harmful pathogens.

1 is a flow chart schematically showing a method for preparing an antibacterial composition comprising a plant extract as an active ingredient according to the present invention.
Figure 2 is a test report evaluated by the FITI Test Research Institute for the bactericidal power of the antibacterial composition comprising a plant extract according to the present invention as an active ingredient.
3 to 8 show the results of a sterilization test of an antimicrobial composition comprising a plant extract according to the present invention as an active ingredient, and FIG. 3 is a photograph showing the results of a sterilization test against Staphylococcus aureus ATCC 6538, FIG. 4 is a photograph showing the results of a sterilization test for Escherichia coli ATCC 8739, Figure 5 is a photograph showing the results of a sterilization test for Klebsiella pneumoniae ATCC 4352, Figure 6 is a photograph showing the results of a sterilization test for Klebsiella pneumoniae ATCC 4352, Figure 6 is a picture showing Salmonella typhimurium KCTC 1925 A photograph showing the sterilization test results for, Figure 7 is a photograph showing the sterilization test results for Pseudomonas aeruginosa ATCC 27853, Figure 8 is a photograph showing the sterilization test results for Staphylococcus aureus ATCC 33591.
Figure 9 is a test report evaluated by the FITI Test Research Institute for the deodorizing power of the antibacterial composition comprising the plant extract according to the present invention as an active ingredient.

Hereinafter, preferred embodiments according to the present invention will be described in detail with reference to the accompanying drawings. In addition, in this embodiment, the scope of the present invention is not limited, it is presented only as an example, and various changes are possible within a range that does not deviate from the technical gist.

Since the present invention can make various changes and have various embodiments, specific embodiments are illustrated in the drawings and described in the detailed description.

However, this is not intended to limit the present invention to specific embodiments, and should be understood to include all modifications, equivalents, and substitutes included in the spirit and scope of the present invention. Like reference numerals have been used for like elements throughout the description of each figure.

Terms used herein are used only for the purpose of distinguishing one component from another. For example, a second element may be termed a first element, and similarly, a first element may be termed a second element, without departing from the scope of the present invention. The terms and/or include any combination of a plurality of related recited items or any of a plurality of related recited items.

Terms used in this application are only used to describe specific embodiments, and are not intended to limit the present invention. Singular expressions include plural expressions unless the context clearly dictates otherwise. In this application, the terms "include" or "have" are intended to designate that there is a feature, number, step, operation, component, part, or combination thereof described in the specification, but one or more other features It should be understood that the presence or addition of numbers, steps, operations, components, parts, or combinations thereof is not precluded.

Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which the present invention belongs. Terms such as those defined in commonly used dictionaries should be interpreted as having a meaning consistent with the meaning in the context of the related art, and unless explicitly defined in this application, it should not be interpreted in an ideal or excessively formal meaning. don't

Hereinafter, preferred embodiments of the present invention will be described with reference to the accompanying drawings. Figure 1 is a flow chart schematically showing a method for producing an antibacterial composition comprising a plant extract according to the present invention as an active ingredient, Figure 2 is a FITI Test Research Institute showing the bactericidal power of the antibacterial composition comprising a plant extract according to the present invention as an active ingredient 3 to 8 show the results of a sterilization test of an antibacterial composition comprising a plant extract according to the present invention as an active ingredient, and FIG. 3 is a sterilization against Staphylococcus aureus ATCC 6538. A photograph showing the test results, Figure 4 is a photograph showing the sterilization test results for Escherichia coli ATCC 8739, Figure 5 is a photograph showing the sterilization test results for Klebsiella pneumoniae ATCC 4352, Figure 6 is Salmonella (Salmonella typhimurium KCTC 1925), Figure 7 is a picture showing the sterilization test results for Pseudomonas aeruginosa ATCC 27853, Figure 8 is a sterilization test for Staphylococcus aureus ATCC 33591 A photograph showing the result, and Figure 9 is a test report evaluated by the FITI Test Research Institute for the deodorizing power of the antibacterial composition containing the plant extract according to the present invention as an active ingredient.

As shown in the drawing, the method for preparing an antibacterial composition containing a plant extract as an active ingredient according to the present invention is extracted from a cypress tree, but contains a phytoncide-containing cypress tree extract, a moss extract extracted from moss, and a pogongyoung extract from Pogongyoung. It was prepared by mixing the extracts.

To this end, the present invention provides a method for preparing an antibacterial composition comprising a plant extract as an active ingredient comprising the following steps.

First, among the leaves, stems and roots of the cypress tree, clean ones that are not eaten by insects are selected, and the leaves, stems and roots of the selected cypress tree are pulverized with a grinder, but pulverized to a size of 0.25 cm to 1.5 cm.

Preferably, the leaves, stems and roots of the cypress tree are ground to a size of 0.5 cm to 1 cm.

As described above, the raw material of the crushed cypress tree was washed and rinsed with running water three or more times to cleanly remove foreign substances such as soil from the leaves, stems and roots of the cypress tree, and then the raw material of the cypress tree from which foreign substances were removed. In order to remove the moisture remaining in the cypress tree, the crushed raw material of the cypress tree is put into a dryer, and then dried by blowing at a temperature of 15 ° C to 45 ° C for 3 to 12 hours.

Preferably, the cypress wood pulverized raw material is put into a dryer and dried by blowing at a temperature of 25 ° C to 30 ° C for 8 hours.

In this way, after removing the moisture remaining in the raw cypress tree raw material, the water-removed crushed raw material of the cypress tree is dried in a natural state for 5 to 10 days at room temperature.

Preferably, the pulverized raw material of cypress tree from which moisture has been removed is dried in a natural state for 7 to 8 days.

In addition, the dried cypress wood pulverized raw material is again pulverized through a grinder to have a particle size of 30 mesh to 500 mesh.

Preferably, the raw material of cypress tree pulverization dried in a natural state is pulverized again to have a particle size of 50 mesh to 300 mesh through a pulverizer.

The pulverized cypress tree raw material powder is added to a stirrer in a weight ratio of 1: 100 with ethanol and stirred, but stirred at a temperature of 20 ° C to 50 ° C at a low speed of 50 rpm to 400 rpm for 20 to 30 hours.

Preferably, the pulverized cypress tree raw material powder is added with ethanol in a stirrer at a weight ratio of 1: 100 and stirred, and mixed by stirring at a temperature of 35 ° C to 40 ° C at a low speed of 100 rpm to 300 rpm for 24 hours.

In one embodiment of the present invention, after crushing the leaves, stems and roots of the cypress tree to a certain size, the crushed leaves, stems and roots are washed, dried, and then pulverized into powder through a grinder, and then powdered raw material of the cypress tree The powder is stirred and mixed with ethanol, but the fruit of the cypress tree is also pulverized together with the leaves, stems and roots to a certain size, and then the pulverized fruit, leaves, stems and roots are washed and dried, and then pulverized into powder through a grinder. Then, it is also possible to mix the powdered cypress raw material powder with ethanol by stirring.

Here, since the phytoncide content of the cypress tree is in the order of fruit, root, leaf, and stem, in order to adjust the phytoncide content according to the place of use and environment, only the fruit of the cypress tree, only the leaves, or only the stem are selected. , It is also possible to select only the root and then pulverize it to prepare raw powder of cypress tree, and then mix it with ethanol by stirring, but it is not limited thereto.

In this way, the mixed solution of the cypress tree raw material powder and ethanol is primarily filtered through a filter to separate only the liquid components except for the solid components.

Then, the mixture of liquid components from which the solid content has been removed is filtered again through a second filter to remove residual debris included in the mixture of liquid components.

Here, it is preferable to filter the mixed solution through the installed filter paper after installing the filter paper in the funnel when filtering the mixed solution of the mixture of the cypress tree raw material powder and ethanol, but is not limited thereto.

In this way, the cypress filtrate filtered through the first and second filtration processes is precipitated for 18 to 30 hours, filtered under reduced pressure, and the cypress filtrate filtered under reduced pressure is put into a rotary evaporator and then added to the cypress filtrate. The contained ethanol and purified water are evaporated to remove impurities.

Then, solvents such as ethanol and purified water are evaporated through the rotary evaporator to remove impurities, and then the concentrated cypress tree concentrate is placed in an oven at 30° C. to 120° C. for 18 to 30 hours at 1 to 3 atm. It is dried to obtain a cypress tree extract, that is, phytoncide oil.

Preferably, solvents such as ethanol and purified water are evaporated through the rotary evaporator to remove impurities, and then the concentrated cypress tree concentrate is placed in an oven at 50 ° C to 100 ° C and dried at 1 atm for 24 hours to obtain a cypress tree extract. to obtain

On the other hand, since the leaves and stems of the moss plants are not clearly distinguished, after screening clean moss, the selected moss is pulverized with a grinder and pulverized to a size of 0.25 cm to 1.5 cm.

Preferably, the moss is also ground to a size of 0.5 cm to 1 cm in the same way as the leaves, stems and roots of the cypress tree.

As described above, the pulverized moss pulverized raw material is washed and rinsed with running water 2 to 3 times to remove foreign substances cleanly, and then the moss pulverized raw material is dried in a dryer to remove the moisture remaining in the moss pulverized raw material from which foreign substances are removed. After adding, it is dried by blowing at a temperature of 10 ° C to 30 ° C for 1 hour to 5 hours.

Preferably, after putting the moss pulverized raw material into a dryer, it is dried by blowing for 3 hours at a temperature of 15 ° C to 25 ° C.

In this way, after removing the moisture remaining in the pulverized moss raw material, the pulverized moss raw material from which the moisture has been removed is dried in a natural state for 1 to 2 days at room temperature.

Preferably, the moss pulverized raw material from which moisture has been removed is dried in a natural state for about 1 day at room temperature.

In addition, the moss ground material dried in a natural state is again ground through a grinder to have a particle size of 30 mesh to 500 mesh, preferably, the ground moss ground material is ground through a grinder to have a particle size of 50 mesh to 300 mesh. do.

The pulverized moss raw material powder is added to a stirrer with ethanol at a weight ratio of 1:100 and stirred, and stirred at a temperature of 10 ° C to 30 ° C at a low speed of 50 rpm to 400 rpm for 10 to 18 hours.

Preferably, the pulverized moss raw material powder is added with ethanol in a stirrer at a weight ratio of 1: 100 and stirred, and mixed by stirring at a temperature of 15 ° C to 25 ° C at a low speed of 100 rpm to 300 rpm for 14 hours.

In this way, the mixture of the moss raw material powder and ethanol is firstly filtered through a filter to separate only the liquid components except for the solid content, and then the mixed solution of the liquid components from which the solid content is removed is filtered through the filter for the second time to separate the liquid component mixture. Remove residual debris contained in

In addition, the moss filtrate filtered through the first and second filtration processes is precipitated for 8 to 20 hours, filtered under reduced pressure, and the moss filtrate filtered under reduced pressure is put into a rotary evaporator, followed by ethanol contained in the moss filtrate. and evaporating purified water to remove impurities.

Then, solvents such as ethanol and purified water are evaporated through the rotary evaporator to remove impurities, and then the concentrated moss concentrate is placed in an oven at 15° C. to 100° C. for 12 to 24 hours at 1 to 3 atm. Dry to obtain a moss extract.

Preferably, solvents such as ethanol and purified water are evaporated through the rotary evaporator to remove impurities, and then the concentrated moss concentrate is placed in an oven at 30 ° C to 80 ° C and dried at 1 atm for 18 hours to obtain a moss extract. do.

Here, moss plants collectively refer to silver plants belonging to selective plants, and grow on rocks and wetlands as lower plants without clear distinction between leaves and stems and without vascular bundles.

These moss plants are natural air purifiers with air purifying abilities, and are known to have 1,000 times higher air purifying abilities than general air purifying plants, and generate a greater amount of phytoncide than phytoncide generated from cypress trees. It releases oxygen and negative ions.

In addition, moss plants not only act as natural air purifiers that suck in fine dust, but also relieve stress, extend sleep time by sedating, and regulate blood pressure.

In addition, moss plants have antibacterial and deodorizing effects, improve skin diseases such as rhinitis, allergy and atopy prevention, and purify by absorbing and removing toxic pollutants including arsenic and lead.

Here, the moss plant is preferably composed of one or more of pine moss, umbrella moss, and horn moss, but the moss plant inhibits factors that cause itching and pain and is more effective in anti-inflammatory effects. desirable.

On the other hand, after selecting the clean Pogong Young, the selected Pogong Young is pulverized with a grinder and pulverized to a size of 0.25 cm to 1.5 cm.

Preferably, the pogongyoung is also pulverized to a size of 0.5 cm to 1 cm in the same way as the cypress tree and moss.

Here, Pogongyeong (蒲公英) is a dried outpost of dandelion in the Asteraceae family, which acts as a natural antibiotic to suppress bacteria, strengthens the immune function, and acts to clear the blood and detoxify.

These, Pogongyeong lower fever poison, eliminate boils, and are used for the treatment of swelling, mastitis, sore throat and carbuncle, etc., useful for symptoms such as eye congestion, acute hepatitis, jaundice, etc., and for liver function protection and diuretic action It is useful, has anti-inflammatory action, gastric action, choleretic action, diuretic action, and immunostimulating action, and is used for tonsillitis, hepatitis, cholecystitis, gastritis, acute bronchitis, indigestion, and is used as a stomachic and antipyretic agent.

In addition, it is effective for pneumonia, bronchitis, hepatitis, pancreatitis, tonsillitis, tuberculosis, cold, otitis media, renal failure, gastric ulcer, and breast cancer. It strengthens the stomach and is used for indigestion, anorexia, vomiting, diarrhea, and constipation. It is effective in preventing and treating various liver diseases, including fatty liver and liver cirrhosis, by promoting bile secretion.

In addition, Pogongyeong promotes the proliferation of epithelial cells and is effective in wound healing of the skin.

As described above, after washing and rinsing the pulverized raw material of Pogongyeong 2 to 3 times with water to cleanly remove foreign substances, the raw material of Pogongyeong pulverized to remove the remaining moisture in the removed foreign substances is put into a dryer. After that, it is dried by blowing at a temperature of 10 ° C to 30 ° C for 1 hour to 3 hours.

Preferably, the pulverized raw material of Pogongyeongyeong is put into a dryer and then dried by blowing air at a temperature of 15°C for 3 hours.

In this way, after removing the moisture remaining in the raw material of Pogongyeong, the raw material from which moisture has been removed is dried in a natural state for 1 to 2 days at room temperature.

Preferably, the pulverized raw material of Porconias from which moisture has been removed is naturally dried at room temperature for about 1 day.

Then, the raw material of Pogongyeong dried in a natural state is again pulverized through a grinder to have a particle size of 30 mesh to 500 mesh, preferably, the raw material of Pogongyeong is pulverized through a grinder to have a particle size of 50 mesh to 300 mesh. .

The pulverized Porconia root powder is added to a stirrer with ethanol at a weight ratio of 1:100 and stirred, stirring at a temperature of 10 ° C. to 30 ° C. at a low speed of 50 rpm to 400 rpm for 8 to 12 hours.

Preferably, the pulverized raw material powder of Porconia persimmon is added to a stirrer with ethanol at a weight ratio of 1:100 and stirred, and stirred at a temperature of 15°C to 25°C at a low speed of 100 rpm to 300 rpm for 10 hours.

In this way, the mixed solution of the mixture of the raw material powder and ethanol of Porconia chinensis is firstly filtered through a filter to separate only the liquid components except for the solid content, and then the mixed solution of the liquid components from which the solid content is removed is filtered through the filter again for the second time to separate the liquid component mixture. Remove residual debris contained in

In addition, the filtrate of Porconias filtered through the first and second filtration processes is precipitated for 8 to 20 hours, filtered under reduced pressure, and the filtrate of Porconias filtered under reduced pressure is put into a rotary evaporator, followed by the ethanol contained in the filtrate of Porconias. and evaporating purified water to remove impurities.

Then, solvents such as ethanol and purified water are evaporated through the rotary evaporator to remove impurities, and then the concentrated Pogongyeong concentrate is placed in an oven at 15° C. to 100° C. for 8 to 18 hours at 1 to 3 atm. Drying to obtain Porconia extract.

Preferably, solvents such as ethanol and purified water are evaporated through the rotary evaporator to remove impurities, and then the concentrated Pogongyoung concentrate is placed in an oven at 30°C to 80°C and dried at 1 atm for 12 hours to obtain an extract of Pogongyoung. do.

As described above, after obtaining the cypress extract, moss extract, and Pogongyoung extract from the cypress tree, moss plant, and Pogongyoung (S10), each of the obtained cypress extract, moss extract, and Pogongyoung extract was extracted at low temperature in vacuum with water or alcohol. However, each extract is extracted with water or alcohol at a low temperature in a vacuum at a weight ratio of 1: 1 to 20 (S30).

At this time, the cypress tree extract, moss extract, and pogongyoung extract are mixed with water or alcohol at a weight ratio of 1: 1 to 20, and then extracted under vacuum at a temperature range of 40 ° C to 90 ° C for 30 minutes to 1 hour.

Here, when obtaining the cypress extract, moss extract, and pogongyoung extract from the cypress tree, moss, and pogongyoung, the cypress extract can also be extracted by steam extraction.

That is, when extracting the cypress tree extract from the raw material of the cypress tree, the steam extraction method is used to extract the cypress tree extract at a low temperature. After applying steam for about 6 to 10 hours under a temperature condition of 50 ° C to 70 ° C, it is condensed with cooling water through a steam tower, and it is also possible to receive the condensate and use it as a cypress tree extract. At this time, it is preferable to apply steam for at least 6 hours or more, and deep sea water may be used as a solvent.

In addition, it is also possible to extract the moss extract and Pogongyeong yeong extract by a hot water extraction method.

In other words, when extracting moss extract or Pogongyeong Young extract, moss extract or Pogongyeong pulverized raw material is added to purified water or 50% to 70% fermented alcohol, and then extracted without pressure for 6 hours or more under a temperature condition of 70 ° C to 80 ° C. And it is also possible to obtain a pogongyoung extract.

In addition, 5 to 10 times (v / w) of 50% to 70% fermented alcohol was added to each of the pulverized moss or pulverized Pogongyoung raw materials, followed by repeated extraction twice for 6 hours, followed by suction filtration, and the filtrate at 300 rpm. It is also possible to obtain a moss extract and a Pogongyoung extract by centrifugation at low speed and concentration under reduced pressure, but it is not limited thereto.

Here, in the case of fermenting alcohol using ethanol for the moss or pogongyeong, alcohol can be extracted by adding one or more solvents selected from C1 to C4 lower alcohols or a mixed solvent thereof.

As described above, the extracted cypress extract, moss extract, and pogongyoung extract can be sterilized through an ultraviolet sterilizer or a far-infrared ray sterilizer.

In this way, the cypress concentrate, moss concentrate, and pogongyeong concentrate obtained by vacuum low-temperature extraction are cooled for 2 to 4 hours at 1 ° C to 10 ° C through a low-temperature cooler (S50), and each cooled concentrate is distilled (S70). extracts, moss extracts and pogongyeongyeong extracts are prepared.

The cypress extract, moss extract, and Pogongyoung extract obtained as described above are put into a mixing tank together with water or ethanol and added to the mixing tank (S90).

Here, based on 100% by weight of the total, 50% to 85% by weight of water or ethanol, 5% to 30% by weight of the cypress extract obtained, 3% to 10% by weight of the obtained moss extract, and the obtained Pogongyoung 1% to 10% by weight of the extract is added and mixed.

Preferably, 10% by weight of the cypress extract obtained, 5% by weight of the obtained moss extract, 2.5% by weight of the obtained Pogongyoung extract, and 82.5% by weight of water or ethanol are added and mixed, but it is not limited thereto, and may be modified. .

On the other hand, additional sub-materials may be added when mixing by adding the cypress extract, moss extract, and Pogongyoung extract to water or ethanol.

Here, water-soluble silicate is further added as an auxiliary material, and 0.1% to 1% by weight of the water-soluble silicate is added.

At this time, the water-soluble silicate is composed of pulverized silicate powder after calcining silica at 1500 to 1750 ° C., dissolving silicate in water to make a silicate solution, distilling it under reduced pressure to prepare a porous silicate body.

On the other hand, water-soluble silicate is non-toxic, adsorbs, decomposes, and neutralizes harmful components, and contains essential trace elements to activate beneficial microorganisms.

In one embodiment of the present invention, when the cypress extract, moss extract, and Pogongyoung extract are added to water or ethanol and mixed, water-soluble silicate powder is added as a sub-material, but as a sub-material, additives in addition to the silicate powder can be further added and mixed. .

Here, as an additive, at least one of jasmine powder, peppermint powder, and pine needle powder is further added.

Jasmine (jasmine) is an evergreen shrub, has anticancer and antibacterial effects, stabilizes nerves, relieves stress, relieves anxiety symptoms such as depression because of its good fragrance, and is used as a spice because of its strong aroma.

Peppermint has anti-inflammatory action against bacteria, removes itching and inflammation, relieves anxiety or depression caused by stress, stabilizes the mind and body, helps relieve stress, improves insomnia, and adds a sense of refreshment.

Pine needles are leaves of pine trees, and contain acetylcholine, a brain neurotransmitter that activates the brain, relieving brain fatigue, activating blood circulation, supplying nutrients to brain cells, and enhancing metabolic function, making brain healthy. help in

In addition, it removes active oxygen, acts as an antioxidant, and absorbs heavy metals.

An additive consisting of any one or more of jasmine powder, peppermint powder or pine needle powder having the above effect is added in an amount of 0.5% to 1% by weight.

On the other hand, in one embodiment of the present invention, an additive consisting of any one or more of jasmine powder, peppermint powder, or pine needle powder is added, but astragalus powder or quince powder may be further added.

Astragalus enhances immunity, such as improving resistance to external viruses through antioxidant and anti-inflammatory effects, and helps to improve health by lowering blood pressure, diuretic, tonic, and blood circulation.

Quince helps with bronchial diseases and asthma, helps recover from fatigue and boosts immunity.

Here, 0.01% to 0.05% by weight of astragalus powder and 0.01% to 0.03% by weight of quince powder are added based on 100% by weight of the total.

In addition, it is possible that eucalyptus powder, radish root extract, olive extract, sandalwood extract, and agarwood powder are further added.

Eucalyptus is an evergreen tree of the dicotyledonous myrtle myrtle family. It has anti-rhinitis, anti-inflammatory action that helps bronchial tubes, deodorizing function, stress reduction, improving immunity, and helping the respiratory system.

In addition, it has a strong bactericidal action and is also used as a bathing agent, and has an effect of killing pests such as fleas and lice.

Daecheong is a biennial plant, as an antibacterial, anti-inflammatory and antiviral plant, effective against infections such as colds, pneumonia, and viral hepatitis, and helps with inflammatory skin diseases.

Olives are rich in antioxidants, help create cholesterol, keep blood vessels clean, and prevent cardiovascular diseases such as high blood pressure and arteriosclerosis.

Here, the olive extract is preferably one extracted from olive fruit, but is not limited thereto.

Sandalwood is the heartwood of danhyang and tree trunks, has a clear scent, and has a sedative effect to relieve tension and anxiety.

In addition, sandalwood has sedative and anti-inflammatory properties, so it increases immunity against cough and sore throat, prevents infection, relieves symptoms, and promotes sleep.

Agarwood is an evergreen tree of the Aloe vera family, which helps with blood circulation and helps brain health by activating brain neurotransmitters as it contains delta guayene.

Here, 0.1% to 0.3% by weight of eucalyptus powder, 0.001% to 0.005% by weight of Daecheong root extract, 0.005% to 0.01% by weight of olive extract, 0.0005% to 0.001% by weight of sandalwood extract, based on 100% by weight of the total 0.001% to 0.003% by weight of aloes powder is added.

In addition, it is also possible that lavender powder and lemon powder are further added.

Lavender harmonizes the nervous system, regulates stress, promotes vigor, strengthens nerves, and calms down.

In addition, lavender has a great effect on insomnia, especially insomnia caused by mental stress and anxiety, prevents infection, has antiseptic, analgesic, and cell regeneration promoting properties that help prevent infection and help quick healing, and is effective against colds, influenza, bronchitis, sore throat, And it can be applied to the treatment of catarrhal symptoms.

Lemon is rich in liminoids, which are antioxidants, so it helps to suppress active oxygen and aging, and it helps immunity, so it is often applied to detox products.

Here, 0.015% to 0.08% by weight of lavender powder and 0.001% to 0.01% by weight of lemon powder are added based on 100% by weight of the total.

As described above, the plant-extracted antibacterial composition can be prepared by adding the cypress extract, moss extract, and Pogongyeongyeong extract to ethanol in a mixing tank, and then adding sub-materials and / or additives and mixing.

Here, when mixing by stirring the mixture added and added into the mixing tank, it may be mixed for 1 hour to 3 hours at a temperature range of 60 ° C to 90 ° C.

Then, the mixed liquid is sterilized by heating at a temperature of 95° C. or higher for 15 to 30 minutes, and cooled at room temperature.

After storing the prepared plant-extracted antibacterial composition in a storage container of an aerosol-type spraying device, it is sprayed to the outside through a spraying nozzle provided in the spraying device, and various bacteria, harmful pathogens and bacteria are sprayed on the target or target place for use. It can prevent the reproduction and transmission of children.

In addition, a large amount of phytoncide is generated, negative ions are discharged to purify the air, and since ingredients extracted from natural plants are used as the main raw material, it is harmless to the human body and can be used for companion animals.

Experimental Example 1: Sterilization power test of antibacterial composition

The bactericidal power of the plant-extracted antibacterial composition according to this embodiment was tested. At this time, as the strains to be tested, Staphylococcus aureus ATCC 6538, Staphylococcus aureus ATCC 33591), Escherichia coli (Escherichia coli ATCC 8739), Klebsiella pneumoniae ATCC 4352, Salmonella (Salmonella typhimurium KCTC 1925) and Pseudomonas aeruginosa ( pseudomonas aeruginosa ATCC 27853) was used.

After culturing each strain to be tested in a liquid medium (TRYPTIC SOY AGAR), the cultured bacteria were diluted to adjust the initial inoculation strain to 1 × 10 ⁵ CFU / mL to 9 × 10 ⁵ CFU / mL.

20 mL of the plant-extracted antibacterial composition according to this embodiment was put in a sterilized cap tube, each cultured bacteria was diluted to adjust the initial inoculation strain, and the number of bacteria after 5 minutes of inoculation at room temperature was measured to reduce the initial number of bacteria. was measured.

However, in the initial dilution step, the experiment was performed through a neutralization process using the D/E NEUTRALIZING BROTH (DIFCO) neutralization method.

When the bacteria grew in the medium, the value was calculated by multiplying the strain on the medium by the dilution factor, and when the bacteria did not grow in the medium (if the bacteria showed 99.9% or higher bactericidal power), the value was multiplied by the dilution factor made in the neutralization step to “less than 10”. ” is indicated.

At this time, the reduction rate (99.9% or more) of the strain measured after 5 minutes was calculated from the relational expression of {(A-B)/A} x 100. A is the initial inoculated strain, and B is the strain measured after 5 minutes.

As a result of the experiment, as shown in FIG. 2, when the plant-extracted antibacterial composition according to this example was exposed for 5 minutes, all of the strains to be tested did not proliferate, and accordingly, as shown in FIGS. 3 to 8, this embodiment It can be seen that the plant-extracted antibacterial composition according to the example is inhibited by 99.9% or more against bacteria and pathogenic harmful bacteria, and thus it can be confirmed that it has excellent bactericidal power.

Experimental Example 2: Deodorization test of antibacterial composition

The plant-extracted antibacterial composition according to the present embodiment is effective against ammonia, acetic acid, methylmethane, trimethylamine, formaldehyde, acetaldehyde, benzene and toluene, which are gases with strong odor components such as toilet odor, sweat odor, food odor, etc. The deodorizing ability was tested.

As a result of the experiment, as shown in FIG. 9, in the case of the plant-extracted antibacterial composition according to this embodiment, after 30 minutes of mixing with ammonia and acetic acid, 99.6% and 99.7% of inhibitory ability against ammonia and acetic acid after 30 minutes was confirmed. could

Examples and Comparative Examples

Hereinafter, the present invention will be described in detail with examples and comparative examples. An average person skilled in the art to which the present invention belongs may change the present invention in various other forms other than the compositions described in the examples below, and the examples below only illustrate the present invention, but the scope of the technical idea of the present invention is limited to the following examples. It should not be construed as intended to limit the scope.

Example 1

In the mixing tank, 825 ml of ethanol, 100 ml of cypress tree extract, 50 ml of moss extract, and 25 ml of Pogongyoung extract were added and mixed. After mixing at a temperature of 75 ° C for 3 hours, the mixed solution was sterilized by heating at a temperature of 97 ° C or higher for 15 minutes. Treatment, and cooling treatment at room temperature to prepare a plant-extracted antibacterial composition.

The prepared plant-extracted antibacterial composition was used by storing it in a storage container of an injector.

Example 2

A plant-extracted antibacterial composition was prepared in the same process as in Example 1, except that 500ml of ethanol, 300ml of cypress extract, 100ml of moss extract, and 100ml of Pogongyeongyeong extract were added to the mixing tank.

Example 3

A plant-extracted antibacterial composition was prepared in the same process as in Example 1, except for adding 910ml of ethanol, 50ml of cypress extract, 30ml of moss extract, and 10ml of Pogongyeongyeong extract in the mixing tank.

Example 4

A plant-extracted antibacterial composition was prepared in the same process as in Example 1, except for adding 300ml of ethanol, 350ml of cypress extract, 200ml of moss extract, and 150ml of Pogongyeongyeong extract in the mixing tank.

Example 5

A plant-extracted antibacterial composition was prepared in the same process as in Example 1, except for adding 950ml of ethanol, 30ml of cypress extract, 15ml of moss extract, and 5ml of Pogongyeongyeong extract in the mixing tank.

Comparative Example 1

Commercially available "cypress water phytoncide undiluted solution" was put into a spray atomizing device and used.

Comparative Example 2

Commercially available "cypress tree spray leaf sap phytoncide" was used by putting it in a spray atomizing device.

division scent preference stimulation freshness vitality overall sign Example 1 4.7 4.5 4.8 4.7 4.7 Example 2 4.5 4.7 4.6 4.6 4.5 Example 3 4.5 4.3 4.4 4.4 4.4 Example 4 4.4 4.8 4.5 4.5 4.4 Example 5 4.3 4.2 4.3 4.3 4.3 Comparative Example 1 4.3 4.4 4.2 4 4.1 Comparative Example 2 4.3 4.3 4.1 3.9 4.1

As shown in [Table 1], after 30 minutes after spraying the plant-extracted antibacterial composition prepared in Examples 1 to 5 and the phytoncide antibacterial product according to Comparative Examples 1 and 2 to bedding three times every 10 minutes, The test was conducted, and it was conducted on 30 adults in their 20s to 60s, and the score and evaluation criteria were conducted on a 5-point scale.

As a result of the experiment, it was confirmed that the plant-extracted antibacterial composition prepared in Examples 1 to 3 was superior to the plant-extracted antibacterial composition prepared in Examples 4 and 5 in fragrance preference, stimulation, freshness, durability, and overall preference. , In the case of Examples 2 and 4, it can be seen that the cypress extract, moss extract, and Pogongyoung extract are contained in a large amount, and the fragrance stimulation is relatively high. In the case of Examples 3 and 5, the cypress extract, moss extract, and Pogongyoung extract are It can be seen that it is contained in a small amount, so the fragrance is relatively light, and the freshness and durability are reduced.

In addition, it was confirmed that compared to Examples 1 to 5, compared to Comparative Examples 1 and 2, fragrance preference, stimulation, freshness, durability, and overall preference were excellent. It is judged that this contains moss extract and pogongyoung extract in addition to the cypress tree extract to satisfy consumers' preferences.

Therefore, it was confirmed that the plant-extracted antibacterial composition according to the present invention contains moss extract and pogongyeong extract in addition to the cypress tree extract, and thus has a superior phytoncide content compared to existing products, and thus has higher marketability than existing products.

In the above, the present invention has been shown and described in relation to specific embodiments, but it is common knowledge in the art that various modifications and changes are possible within the limits that do not depart from the spirit and scope of the invention shown in the appended claims. Anyone who has it will be able to easily understand.

Claims (6)

Obtaining a cypress tree extract, a moss extract, and a Pogongyoung extract from a cypress tree, a moss plant, and a Pogongyoung plant (S10);
Vacuum low-temperature extraction of each of the obtained cypress extract, moss extract, and Porconia extract (S30);
Cooling the extract of cypress cypress, moss extract, and Porconia chinensis extract extracted at low temperature under vacuum (S50);
Distilling the cooled cypress extract, moss extract, and Pogongyoung extract (S70); and
After adding water or ethanol into the mixing tank, adding and mixing the distilled cypress extract, moss extract, and Pogongyoung extract (S90); including,
The vacuum low-temperature extraction step (S30) extracts the cypress tree extract by using a steam extraction method at low temperature when extracting the cypress tree extract from the raw material of the cypress tree. After vacuuming to 0.08 MPa, applying steam for 6 to 10 hours under a temperature condition of 50 ° C to 70 ° C, condensing with cooling water through a steam tower, receiving the condensate and using it as a cypress tree extract,
In the case of extraction of moss extract and Pogongyoung extract, hot water extraction is used for extraction, but moss pulverized raw material is added to purified water or 50% to 70% fermented alcohol, and then extracted without pressure for more than 6 hours under a temperature condition of 70℃ to 80℃. In the case of extracting the extract of Pogongyeong, the pulverized raw material of Pogongyeong is added to purified water or 50% to 70% fermented alcohol, and then extracted without pressure for more than 6 hours under a temperature condition of 70℃ to 80℃.
In the mixing step (S90), 10% by weight of the obtained cypress extract, 5% by weight of the obtained moss extract, 2.5% by weight of the obtained Porconia extract, and 82.5% by weight of ethanol were added to the total 100% by weight in the mixing tank. and mixing, but after mixing at a temperature of 75 ° C. for 3 hours, the mixed solution is sterilized by heating at a temperature of 97 ° C. or higher for 15 minutes, and cooled at room temperature. A method for preparing an antibacterial composition.
delete delete The method of claim 1,
When water or ethanol, cypress extract, moss extract, and Pogongyeongyeong extract are added to the mixing tank, additional materials are added, wherein the auxiliary material is a water-soluble silicate.
The method of claim 4,
Additives are further added when water or ethanol, cypress extract, moss extract, Pogongyoung extract, and auxiliary materials are added in the mixing tank, and at least one of jasmine powder, peppermint powder, or pine needle powder is further added as the additive. Method for producing an antibacterial composition comprising a plant extract as an active ingredient.
Claims 1, claim 4 and claim 5 of any one of the plant extract prepared by the method as an active ingredient characterized in that the extract plant antibacterial composition.

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