KR20220052702A - Manufacturing method of antimicrobial composition containing natural plant extract and antimicrobial composition thereof - Google Patents

Abstract

The present invention relates to a method for preparing an antibacterial composition containing a plant extract as an active ingredient and a plant-extracted antibacterial composition prepared thereby. An objective of the present invention is to provide a method for preparing an antibacterial composition containing a plant extract as an active ingredient and a plant-extracted antibacterial composition prepared thereby, which has excellent antibacterial and deodorizing properties, which are unique characteristics of phytoncide, helps to improve health with antioxidant, antibacterial, anti-inflammatory, anti-stress, and various physiological activities, can deodorize, sterilize, and remove insects such as mites, etc. with its antibacterial action, and can enhance a sense of comfort and soothing due to phytoncide. The method comprises: a step of obtaining a cypress tree extract, a moss extract, and a dandelion extract from cypress trees, moss plants, and dandelions; a step of vacuum low-temperature extraction of the obtained cypress extract, moss extract, and dandelion extract, respectively; a step of cooling the vacuum low-temperature extracted cypress extract, moss extract, and dandelion extract; a step of distilling the cooled cypress extract, moss extract, and dandelion extract; and a step of adding water or ethanol to a mixing tank and then adding and mixing the distilled cypress extract, moss extract, and dandelion extract.

Description

A method for producing an antimicrobial composition comprising a plant extract as an active ingredient, and a plant extract antimicrobial composition prepared accordingly

The present invention relates to a method for preparing an antibacterial composition comprising a plant extract as an active ingredient, and to a plant extract antibacterial composition prepared thereby, and more particularly, to a plant extract comprising a cypress extract, a moss extract and a pogongyeong extract as an active ingredient. It enhances the immune system of the human body, helps to promote health with antioxidant, anti-inflammatory, anti-cancer, anti-stress and various physiologically active functions, and provides deodorization, deodorization and sterilization effects with antibacterial action, and provides comfort and calming effect due to phytoncide It relates to a method for preparing an antibacterial composition comprising a plant extract capable of enhancing the senses as an active ingredient, and to a plant extract antibacterial composition prepared thereby.

In recent years, fine dust has emerged as a social problem in an environment where air pollution has become severe and yellow dust is frequently generated.

Fine dust is divided into fine dust with a particle size smaller than 10㎛ and ultrafine dust smaller than 25㎛, and its composition may vary depending on the region of occurrence, season, and weather conditions.

Since the particle size of such fine dust is very small, it can stay in the atmosphere and penetrate the lungs through the human respiratory system, or move into the body through blood vessels, thereby adversely affecting health.

On the other hand, when microorganisms or bacteria ingest and decompose contaminants such as sweat or sebum secreted by the body, inflammation is induced by strong skin irritation, and bacteria such as Staphylococcus and Escherichia coli can cause a strong odor.

In particular, because certain parts of the body have favorable humidity and temperature conditions for bacteria to thrive, various microorganisms such as bacteria, fungi, pathogenic bacteria, athlete's foot, and mold can breed, which causes skin irritation, itchiness, Inflammation, blisters, rashes, and odors may be produced.

In addition, when various microorganisms actively reproduce in the clothes, bedding, and shoes used, severe odors may be generated, and the environment of group living such as military bases, dormitories, and training centers where a large number of people live together as a group The frequency of exposure to various microorganisms harmful to the body increases, causing infections and bad odors.

Various antibacterial agents have been developed to purify fine dust as described above and inhibit the propagation and proliferation of various microorganisms. Chemical cleaners for this purpose, and drugs for skin diseases to eradicate bacteria that inhabit the human body and provide the cause of inflammation have been developed and used.

However, since antibacterial agents for different uses are required depending on the types of microorganisms, antibacterial agents are needed according to places or objects where bacteria are to be removed, and antibacterial agents harmless to the human body must be provided, so it is not easy to distinguish each antibacterial agent. However, there is a problem that the user's cost burden is increased.

In order to solve the above-mentioned problems, a technology for obtaining antibacterial, insect repellent, aroma, and deodorizing effects through phytoncide, an active ingredient, by preparing a liquid composition of cypress wood is disclosed in Korean Patent Application Laid-Open No. 10-2012-0025740, Technology applied as a deodorant, a fragrance, etc. in the form of a composition blended with other natural oil components is disclosed in Korean Patent No. 10-1439385 and Korean Patent No. 10-1161415.

Phytoncide, an active ingredient of cypress, is a substance with antibacterial, insect repellent, aroma, and deodorizing effects.

Here, phytoncide is a compound word of phyton, meaning plants, and cide, meaning sterilizing power, relieves stress, prevents atopy and allergies, sterilizes, improves immune function, and concentrates Phytoncide prepared from cypress in a liquid composition is sprayed and used because of its excellent deodorizing effect.

In this known technology, although the active ingredient of cypress is included, there is no disclosure on a technique for enhancing the effect by adding a moss extract and a pogongyeong extract to a phytoncide solution.

Accordingly, there is a need for development of a general-purpose antibacterial agent that can be widely used for various purposes and can be used more effectively.

Republic of Korea Patent Registration No. 10-1711050 Republic of Korea Patent Registration No. 10-1698740

The present invention is to solve the above problems, and by preparing an antibacterial composition using a plant extract including a cypress extract, a moss extract and a pogongyeong extract as an active ingredient, the antibacterial and deodorizing properties unique to phytoncide are excellent. It helps to promote health with antioxidant, antibacterial, anti-inflammatory, anti-stress and various physiologically active functions. An object of the present invention is to provide a method for preparing an antibacterial composition comprising a plant extract that can be promoted as an active ingredient and a plant extract antibacterial composition prepared accordingly.

In addition, the present invention is harmless to the human body and animals and can be widely used in the treatment of various skin diseases and atopy that can be caused by various bacteria, bacteria and harmful pathogens, and can be used for exterminating pests such as mites or mosquitoes. , can inhibit the growth of various bacteria and harmful pathogens, and public places such as schools, government offices, churches, rest areas, waiting rooms, elderly homes, hospitals, offices, restaurants, buses, trains, karaoke rooms, homes or toilets where a large number of people gather An object of the present invention is to provide a method for producing an antibacterial composition comprising a plant extract capable of preventing the propagation and transmission of various bacteria and harmful pathogens as an active ingredient, and a plant extract antibacterial composition prepared accordingly.

In addition, the technical problems to be achieved by the present invention are not limited to the technical problems mentioned above, and other technical problems not mentioned are clearly understood by those of ordinary skill in the art to which the present invention belongs from the description below. it could be

In order to achieve the above object, the present invention comprises the steps of: obtaining a cypress extract, a moss extract and a pogongyeong extract from cypress trees and moss plants and pogongyeong; vacuum low temperature extraction of each of the obtained cypress extract, moss extract, and pogongyeong extract; Cooling the vacuum low temperature extracted cypress extract, moss extract and pogongyeong extract; distilling the cooled cypress extract, moss extract, and pogongyeong extract; and adding water or ethanol into the mixing tank and then mixing the distilled cypress extract, moss extract and pogongyeong extract; It is characterized in that it includes.

Here, vacuum low temperature extraction of the obtained cypress extract, moss extract, and pogongyeong extract with water or alcohol at a weight ratio of 1:1 to 20, respectively; further includes

Preferably, based on 100% by weight of the total in the mixing tank, 5% to 30% by weight of the cypress extract obtained in 50% by weight to 85% by weight of water or ethanol, 3% by weight to 10% by weight of the moss extract obtained , and 1 wt% to 10 wt% of the obtained pogongyeong extract are added and mixed.

Here, when water or ethanol, cypress extract, moss extract, and pogongyeong extract are added in the mixing tank, additional materials are added, but the auxiliary material is water-soluble silicate.

In addition, when water or ethanol, cypress extract, moss extract, pogongyeong extract, and auxiliary materials are added in the mixing tank, additives are further added, and as an additive, any one or more of jasmine powder, mint powder, or pine needle powder is further added.

As described above, the present invention having the above configuration is an anti-oxidative, anti-inflammatory, anti-cancer, anti-inflammatory, anti-stress and It helps to promote health with various physiologically active functions, is absorbed into the human body, increases immune function, promotes blood circulation, psychological stability, sterilization and physiological activity, and improves comfort and calmness due to phytoncide, and at the same time enhances the senses of the five senses. It has the effect of satisfying and improving emotional stability.

In addition, the present invention is made of plant extracts and is harmless to the human body and animals, and has excellent antibacterial and deodorizing properties, which are inherent characteristics of phytoncide, so that it is possible to sterilize and remove pests such as mites and mosquitoes. It has an excellent effect and efficacy against insecticide and antibacterial, can neutralize formaldehyde, has an antibacterial effect not only on common bacteria, but also has an excellent antibacterial effect on antibiotic-resistant bacteria (super bacteria), and decomposes odors and odors Rather, it removes the causative bacteria of the odor and decomposes the cause of the odor to fundamentally remove and deodorize the smell of food, toilet, air conditioner, and tobacco, thereby obtaining an air purification effect, and the proliferation of various bacteria, harmful pathogens and bacteria It can be used extensively for various skin diseases including atopy by inhibiting E. coli, Staphylococcus aureus, Pseudomonas aeruginosa and Salmonella, which are harmful to the human body, and is effective in sick house syndrome and new car syndrome.

In addition, the present invention can inhibit the growth of various bacteria, harmful pathogens and bacteria, so schools, government offices, churches, rest areas, waiting rooms, senior citizens' homes, hospitals, offices, restaurants, buses, trains, karaoke, homes or It is used in public places such as toilets and has the effect of preventing the propagation and transmission of various bacteria and harmful pathogens.

1 is a flowchart schematically showing a method for preparing an antibacterial composition comprising a plant extract according to the present invention as an active ingredient.
2 is a test report evaluated by the FITI test researcher on the sterilization power of the antibacterial composition comprising a plant extract according to the present invention as an active ingredient.
3 to 8 show the results of a sterilization test of an antibacterial composition comprising a plant extract according to the present invention as an active ingredient, and FIG. 3 is a photograph showing the results of a sterilization test for Staphylococcus aureus ATCC 6538. 4 is a photograph showing the sterilization test result for Escherichia coli ATCC 8739, FIG. 5 is a photograph showing the sterilization test result for Klebsiella pneumoniae ATCC 4352, FIG. A photograph showing the sterilization test results for, FIG. 7 is a photograph showing the sterilization test results for Pseudomonas aeruginosa ATCC 27853, and FIG. 8 is a photograph showing the sterilization test results for Staphylococcus aureus ATCC 33591.
9 is a test report evaluated by the FITI test researcher on the deodorizing power of the antibacterial composition comprising a plant extract according to the present invention as an active ingredient.

Hereinafter, preferred embodiments according to the present invention will be described in detail with reference to the accompanying drawings. In addition, the present embodiment does not limit the scope of the present invention, but is presented only as an example, and various changes are possible within the scope without departing from the technical gist of the present invention.

Since the present invention can have various changes and can have various embodiments, specific embodiments are illustrated in the drawings and will be described in the detailed description.

However, this is not intended to limit the present invention to specific embodiments, and it should be understood to include all modifications, equivalents and substitutes included in the spirit and scope of the present invention. In describing each figure, like reference numerals have been used for like elements.

The terms used herein are used only for the purpose of distinguishing one component from another. For example, without departing from the scope of the present invention, the second component may be referred to as the first component, and similarly, the first component may also be referred to as the second component. and/or includes a combination of a plurality of related listed items or any of a plurality of related listed items.

The terms used in the present application are only used to describe specific embodiments, and are not intended to limit the present invention. The singular expression includes the plural expression unless the context clearly dictates otherwise. In the present application, terms such as “comprise” or “have” are intended to designate that a feature, number, step, operation, component, part, or combination thereof described in the specification exists, but one or more other features It should be understood that this does not preclude the existence or addition of numbers, steps, operations, components, parts, or combinations thereof.

Unless defined otherwise, all terms used herein, including technical and scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Terms such as those defined in a commonly used dictionary should be interpreted as having a meaning consistent with the meaning in the context of the related art, and should not be interpreted in an ideal or excessively formal meaning unless explicitly defined in the present application. does not

Hereinafter, preferred embodiments of the present invention will be described with reference to the accompanying drawings. 1 is a flowchart schematically showing a method for preparing an antibacterial composition comprising a plant extract according to the present invention as an active ingredient, and FIG. 3 to 8 show the results of a sterilization test of an antibacterial composition comprising a plant extract according to the present invention as an active ingredient, and FIG. 3 is a sterilization for Staphylococcus aureus ATCC 6538. A photograph showing the test result, FIG. 4 is a photograph showing the sterilization test result for Escherichia coli ATCC 8739, FIG. 5 is a photograph showing the sterilization test result for Klebsiella pneumoniae ATCC 4352, FIG. 6 is salmonella (Salmonella typhimurium KCTC 1925) A photograph showing the sterilization test result, FIG. 7 is a photograph showing the sterilization test result for Pseudomonas aeruginosa ATCC 27853, FIG. 8 is a sterilization test for Staphylococcus aureus ATCC 33591) It is a photograph showing the result, and FIG. 9 is a test report evaluated by the FITI test researcher on the deodorizing power of the antibacterial composition comprising a plant extract according to the present invention as an active ingredient.

As shown in the figure, the method for preparing an antibacterial composition comprising a plant extract as an active ingredient according to the present invention is extracted from cypress, but a cypress extract containing phytoncide, a moss extract extracted from moss, and pogongyeong extracted from cypress. It was prepared by mixing the extracts.

To this end, the present invention provides a method for preparing an antibacterial composition comprising a plant extract as an active ingredient, comprising the following steps.

First, clean leaves, stems, and roots of the cypress tree are selected, and the selected leaves, stems and roots of the cypress tree are pulverized with a grinder, and pulverized to a size of 0.25 cm to 1.5 cm.

Preferably, the leaves, stems and roots of the cypress are pulverized to a size of 0.5 cm to 1 cm.

As described above, the pulverized raw material of the cypress tree is washed and washed with running water three times or more to cleanly remove foreign substances such as soil from the leaves, stems and roots of the cypress tree, and then the pulverized raw material of the cypress tree from which the foreign substances are removed In order to remove the remaining moisture in the cypress, the pulverized raw material of cypress is put into a dryer, and then dried by blowing at a temperature of 15° C. to 45° C. for 3 hours to 12 hours.

Preferably, the pulverized cypress raw material is put into a dryer and dried by blowing at a temperature of 25° C. to 30° C. for 8 hours.

In this way, after removing the moisture remaining in the pulverized cypress raw material, the dehydrated pulverized cypress raw material is naturally dried at room temperature for 5 to 10 days.

Preferably, the pulverized cypress raw material from which moisture has been removed is dried in a natural state for 7 to 8 days.

Then, the raw cypress pulverized raw material dried in a natural state is pulverized to have a particle size of 30 mesh to 500 mesh through a pulverizer again.

Preferably, the raw cypress pulverized raw material dried in a natural state is pulverized to have a particle size of 50 mesh to 300 mesh through a pulverizer again.

The pulverized cypress raw material powder is added to a stirrer in a 1:100 weight ratio with ethanol, and stirred, but stirred at a temperature of 20° C. to 50° C. at a low speed of 50 rpm to 400 rpm for 20 to 30 hours.

Preferably, the pulverized cypress raw material powder is added with ethanol in a 1:100 weight ratio in a stirrer and stirred, but stirred at a temperature of 35°C to 40°C at a low speed of 100rpm to 300rpm for 24 hours to mix.

In one embodiment of the present invention, the leaves, stems and roots of cypress are pulverized to a predetermined size, then the pulverized leaves, stems and roots are washed, dried and then pulverized into a powder through a pulverizer, and then powdered raw cypress The powder is mixed with ethanol by stirring, but the fruit of the cypress tree is also crushed to a certain size together with the leaves, stems and roots, and then the crushed fruit, leaves, stems and roots are washed and dried and then pulverized into powder through a grinder Then, it is possible to mix the powdered cypress raw material powder with ethanol by stirring.

Here, since the content of phytoncide of cypress is in the order of fruit, root, leaf and stem, in order to control the content of phytoncide according to the place of use and environment, only the fruit of the cypress is selected, only the leaves are selected, or only the stem is selected, , it is also possible to prepare a raw cypress powder by selecting only the roots and then pulverizing them, and then mixing them with ethanol by stirring, but is not limited thereto.

In this way, the mixed solution in which the raw cypress powder and ethanol are mixed is first filtered through a filter to separate only the liquid components except for the solid content.

Then, the liquid component mixture from which the solids have been removed is secondarily filtered through a filter to remove residual residues contained in the liquid component mixture solution.

Here, when filtering the mixed solution in which the raw cypress powder and ethanol are mixed, a filter paper is installed in the funnel and then the mixed solution is filtered through the installed filter paper, but is not limited thereto.

In this way, the cypress filtrate filtered through the primary and secondary filtration processes is precipitated for 18 to 30 hours, filtered under reduced pressure, and the vacuum-filtered cypress filtrate is put into a rotary evaporator and added to the cypress filtrate. The ethanol and purified water contained therein are evaporated to remove impurities.

Then, the solvent such as ethanol and purified water is evaporated through the rotary evaporator to remove impurities, and then the concentrated cypress concentrate is placed in an oven at 30° C. to 120° C., and at 1 atm to 3 atm for 18 hours to 30 hours. It is dried to obtain a cypress extract, that is, phytoncide oil.

Preferably, the solvent such as ethanol and purified water is evaporated through the rotary evaporator to remove impurities, and then the concentrated cypress concentrate is placed in an oven at 50° C. to 100° C., and dried at 1 atm for 24 hours to extract the cypress tree. to obtain

On the other hand, in the moss plant, since the distinction between leaves and stems is not clear, clean moss is selected and then the selected moss is pulverized with a grinder, and pulverized to a size of 0.25 cm to 1.5 cm.

Preferably, the moss is also pulverized so as to have a size of 0.5 cm to 1 cm in the same way as the leaves, stems and roots of the cypress tree.

As described above, the pulverized moss pulverized raw material is washed with running water 2 to 3 times and washed with water to cleanly remove foreign substances. After input, it is dried by blowing at a temperature of 10° C. to 30° C. for 1 hour to 5 hours.

Preferably, after putting the moss pulverized raw material in a dryer, it is dried by blowing at a temperature of 15° C. to 25° C. for 3 hours.

In this way, after removing the moisture remaining in the moss pulverized raw material, the moss pulverized raw material from which the moisture has been removed is naturally dried at room temperature for 1 to 2 days.

Preferably, the moss pulverized raw material from which moisture has been removed is naturally dried at room temperature for about 1 day.

Then, the raw moss pulverized material dried in a natural state is pulverized to have a particle size of 30 mesh to 500 mesh through a grinder, preferably, the moss pulverized raw material is pulverized to have a particle size of 50 mesh to 300 mesh through a pulverizer do.

The pulverized moss raw material powder is added to a stirrer in a 1:100 weight ratio with ethanol and stirred, but stirred at a temperature of 10° C. to 30° C. at a low speed of 50 rpm to 400 rpm for 10 to 18 hours to mix.

Preferably, the pulverized moss raw material powder is added with ethanol in a 1:100 weight ratio in a stirrer and stirred, but stirred at a temperature of 15° C. to 25° C. at a low speed of 100 rpm to 300 rpm for 14 hours to mix.

In this way, the mixed solution of the moss raw material powder and ethanol is first filtered through a filter to separate only the liquid components except for the solids, and then the mixed solution of the liquid components from which the solids are removed is filtered again through a filter, and the mixture of the liquid components Remove the residual residue contained in the

Then, the moss filtrate filtered through the primary and secondary filtration processes is precipitated for 8 to 20 hours, filtered under reduced pressure, and the moss filtrate filtered under reduced pressure is put into a rotary evaporator and then ethanol contained in the moss filtrate and evaporating purified water to remove impurities.

Then, the solvent such as ethanol and purified water is evaporated through the rotary evaporator to remove impurities, and then the concentrated moss concentrate is placed in an oven at 15° C. to 100° C., and at 1 atm to 3 atm for 12 hours to 24 hours. Drying to obtain a moss extract.

Preferably, the solvent such as ethanol and purified water is evaporated through the rotary evaporator to remove impurities, and then the concentrated moss concentrate is placed in an oven at 30° C. to 80° C., and dried at 1 atm for 18 hours to obtain a moss extract. do.

Here, the moss plant is a common name for silver plants belonging to selective plants, and the distinction between leaves and stems is not clear, and is a lower plant without vascular bundles and grows on rocks and wetlands.

These moss plants are natural air purifiers with air purifying ability, have an air purifying ability about 1,000 times that of general air purifying plants, and are known to generate a greater amount of phytoncides than phytoncides generated from cypress trees. It releases oxygen and negative ions.

In addition, moss plants not only perform the role of a natural air purifier that sucks in fine dust, but also relieve stress, have a calming effect, prolong sleep time, and regulate blood pressure.

In addition, moss plants have antibacterial and deodorizing effects, improve skin diseases such as rhinitis, allergy and atopy prevention, and purify by absorbing and removing toxic pollutants including arsenic and lead.

Here, the moss plant is preferably made of any one or more moss of brush moss, umbrella moss, or horn moss, but the moss plant inhibits factors that cause itching and pain, and is more effective in anti-inflammatory than brush moss. desirable.

On the other hand, after screening the clean pogongyeong, the selected pogongyoung is pulverized with a grinder, and then pulverized to a size of 0.25cm to 1.5cm.

Preferably, the pogongyeong is also pulverized to have a size of 0.5 cm to 1 cm in the same manner as the cypress and moss.

Here, pogongyeong (蒲公英) is a dried outplant of dandelion in the Asteraceae family. It acts as a natural antibiotic to suppress bacteria and strengthens the immune function, purifies the blood and detoxifies.

This, pogongyeong relieves fever and eliminates boils, which is why it is used for the treatment of swelling, mastitis, sore throat, and swelling. It is useful and has anti-inflammatory action, gastric action, biliary action, diuretic action, and immune-stimulating action.

Also, it is effective for pneumonia, bronchitis, hepatitis, pancreatitis, tonsillitis, tuberculosis, cold, otitis media, renal failure, gastric ulcer and breast cancer, etc. It is effective in preventing and treating various liver diseases, including fatty liver and cirrhosis, by promoting bile secretion.

In addition, pogongyeong promotes the proliferation of epithelial cells and is effective in skin wound healing.

As described above, the pulverized raw Pogongyoung pulverized pulverized pulverized pugongyeong pulverized pulverized pulverized pugongyeong pulverized pulverized pulverized raw Pogongyoung pulverized pulverized pulverized raw powgongyeong pulverized raw material is washed 2 to 3 times in water to cleanly remove foreign substances, and then the pulverized Pogongyeong pulverized raw material is put into a dryer to remove moisture remaining in the pulverized Pogongyeong pulverized raw material. After drying, by blowing at a temperature of 10°C to 30°C for 1 hour to 3 hours.

Preferably, after putting the raw material of Pogongyeong pulverized into a dryer, it is dried by blowing at a temperature of 15° C. for 3 hours.

After removing the moisture remaining in the raw pogongyeong pulverized water in this way, the pogongyoung pulverized raw material from which the moisture has been removed is naturally dried at room temperature for 1 to 2 days.

Preferably, the pogongyeong pulverized raw material from which moisture has been removed is naturally dried at room temperature for about 1 day.

Then, the raw Pogongyeong pulverized material dried in a natural state is pulverized to have a particle size of 30 mesh to 500 mesh through a grinder, preferably, the Pogongyeong pulverized raw material is pulverized to have a particle size of 50 mesh to 300 mesh through a pulverizer. .

The pulverized raw material powder of Pogongyeong is added to a stirrer in a 1:100 weight ratio with ethanol and stirred, but stirred at a temperature of 10°C to 30°C at a low speed of 50rpm to 400rpm for 8 hours to 12 hours to mix.

Preferably, the pulverized raw material powder of Pogongyeong is added to ethanol in a weight ratio of 1:100 in a stirrer and stirred, but stirred at a temperature of 15°C to 25°C at a low speed of 100rpm to 300rpm for 10 hours to mix.

In this way, the mixture solution in which the raw powder of Pogongyeong is mixed with ethanol is first filtered through a filter to separate only the liquid components except for the solids, and then the mixed solution of the liquid components from which the solids have been removed is filtered again through a filter, and the mixture of the liquid components Remove the residual residue contained in the

Then, after precipitating the Pogongyoung filtrate filtered through the primary and secondary filtration processes for 8 to 20 hours, filtration under reduced pressure, and putting the reduced pressure filtered Pogongyeong filtrate into a rotary evaporator, and ethanol contained in the Pogongyeong filtrate and evaporating purified water to remove impurities.

Then, the solvent such as ethanol and purified water is evaporated through the rotary evaporator to remove impurities, and then the concentrated Pogongyeong concentrate is placed in an oven at 15° C. to 100° C., and at 1 to 3 atm for 8 hours to 18 hours. Drying to obtain an extract of pogongyeong.

Preferably, the solvent such as ethanol and purified water is evaporated through the rotary evaporator to remove impurities, and then the concentrated Pogongyoung concentrate is placed in an oven at 30°C to 80°C, and dried at 1 atm for 12 hours to obtain a Pogongyeong extract. do.

As described above, after obtaining the cypress extract, the moss extract and the pogongyeong extract from the cypress tree, the moss plant, and the pogongyeong extract (S10), the obtained cypress tree extract, the moss extract and the pogongyeong extract are each extracted at a low temperature in vacuum with water or alcohol. However, each extract is extracted with water or alcohol at a vacuum low temperature in a weight ratio of 1:1 to 20 (S30).

At this time, each of the cypress extract, moss extract, and pogongyeong extract is mixed with water or alcohol in a 1: 1 to 20 weight ratio, and then extracted for 30 minutes to 1 hour at a temperature of 40 ° C. to 90 ° C. under vacuum.

Here, when obtaining the cypress extract, moss extract, and pogongyeong extract from the cypress tree, moss, and pogongyeong, the cypress extract may be extracted by steam extraction.

That is, when extracting the cypress extract from the pulverized cypress raw material, the cypress extract is extracted using the steam extraction method at low temperature, but when the cypress extract is obtained, the washed cypress is vacuumed to a state of 0.06 MPa to 0.08 MPa, After adding steam for about 6 to 10 hours under a temperature condition of 50° C. to 70° C., it is condensed with cooling water through a steam tower, and it is also possible to receive the condensate and use it as a cypress extract. In this case, it is preferable to apply steam for at least 6 hours, and deep sea water may be used as a solvent.

In addition, it is also possible to extract the moss extract and the pogongyeong extract by hot water extraction.

That is, when extracting the moss extract or pogongyeong extract, the moss pulverized raw material or phogongyeong pulverized raw material is added to purified water or 50% to 70% fermented alcohol, and then extracted under pressure at 70°C to 80°C for 6 hours or more. And it is also possible to obtain an extract of pogongyeong.

In addition, 50% to 70% of fermented alcohol in an amount of 5 to 10 times (v/w) is added to each of the moss pulverized raw material or the Pogongyeong pulverized raw material, extracted twice every 6 hours, suction filtration, and the filtrate at 300 rpm It is possible to obtain a moss extract and a pogongyeong extract by centrifuging at a low speed and rotating under reduced pressure, but is not limited thereto.

Here, when fermenting alcohol using ethanol in the moss or pogongyeong, alcohol can be extracted by adding one or more solvents selected from C1 to C4 lower alcohols or a mixed solvent thereof.

As described above, the extracted cypress extract, moss extract, and pogongyeong extract may be sterilized through an ultraviolet sterilizer or a far-infrared sterilizer.

In this way, the vacuum low-temperature extracted cypress concentrate, moss concentrate, and pogongyeong concentrate are cooled at 1°C to 10°C for 2 to 4 hours through a low-temperature cooler (S50), and after distilling each cooled concentrate (S70) cypress Extracts and moss extracts and pogongyeong extracts are prepared.

The cypress extract, moss extract, and pogongyeong extract obtained as described above are added and mixed in a mixing tank together with water or ethanol (S90).

Here, based on 100% by weight of the total, 5% to 30% by weight of the cypress extract obtained in 50% by weight to 85% by weight of water or ethanol, 3% by weight to 10% by weight of the obtained moss extract, and the obtained pogongyeong 1% to 10% by weight of the extract is added and mixed.

Preferably, 10% by weight of the obtained cypress extract, 5% by weight of the obtained moss extract, 2.5% by weight of the obtained pogongyeong extract, and 82.5% by weight of water or ethanol are added and mixed, but not limited thereto, and changes are possible. .

On the other hand, when the cypress extract, the moss extract and the pogongyeong extract are added to water or ethanol and mixed, additional materials may be further added.

Here, as an auxiliary material, water-soluble silicate is further added, and 0.1 wt% to 1 wt% of the water-soluble silicate is added.

At this time, the water-soluble silicate is made of silicate powder by dissolving silicate prepared by calcining silicate at 1500 to 1750° C. in water, distilling it under reduced pressure to prepare a silicate porous body, and then pulverizing silicate powder.

On the other hand, water-soluble silicate is non-toxic, adsorbs, decomposes and neutralizes harmful components, and contains essential trace elements to activate beneficial microorganisms.

In one embodiment of the present invention, when the cypress extract, moss extract, and pogongyeong extract are added to water or ethanol and mixed, a water-soluble silicate powder is added as an auxiliary material. .

Here, as an additive, any one or more of jasmine powder, mint powder, or pine needle powder is further added.

As an evergreen shrub, jasmine has anticancer and antibacterial effects, has a good fragrance, stabilizes nerves, relieves stress, relieves anxiety symptoms such as depression, and is used as a fragrance because of its strong aroma.

Mint has anti-inflammatory action against bacteria, removes itchiness and inflammation, and relieves anxiety or depression caused by stress to stabilize the mind and body, helps relieve stress, improves insomnia, and adds a refreshing feeling.

Pine needles are pine leaves and contain acetylcholine, a brain neurotransmitter that activates the brain to relieve brain fatigue, activate blood circulation to supply nutrients to brain cells, and improve metabolic function for brain health. to help

And, by removing active oxygen, it acts as an antioxidant and absorbs heavy metals.

0.5 wt% to 1 wt% of the additive made of any one or more of jasmine powder, mint powder, or pine needle powder having the above-described efficacy is added.

On the other hand, in an embodiment of the present invention, an additive made of at least one of jasmine powder, mint powder, or pine needle powder is added, but it is also possible that astragalus powder or quince powder is further added.

Astragalus has antioxidant and anti-inflammatory effects, so it increases immunity, such as improving resistance to external viruses, and helps to lower blood pressure, diuresis, tonicity, and blood circulation to help promote health.

Chinese quince helps with bronchial diseases and asthma, and helps to recover from fatigue and boost immunity.

Here, 0.01 wt% to 0.05 wt% of astragalus powder, 0.01 wt% to 0.03 wt% of quince powder are added based on 100 wt% of the total.

In addition, eucalyptus powder, daecheong root extract, olive extract, sandalwood extract, it is also possible to further add aloesthetic powder.

Eucalyptus is a dicotyledonous plant, an evergreen tree of the gilded sheep family. It has anti-rhinitis and anti-inflammatory action to help the bronchial tubes, and has a deodorizing function, reducing stress, improving immunity, and helping the respiratory system.

In addition, it has a strong sterilizing effect and is used as a bathing agent, and has an effect of insecticidal pests such as fleas and lice.

Daechung is a biennial herb, as an antibacterial, anti-inflammatory and antiviral plant, and is effective against infections such as colds, pneumonia, and viral hepatitis, and helps inflammatory skin diseases.

Olives are rich in antioxidants, help to produce cholesterol, keep blood vessels clean, and prevent cardiovascular diseases such as high blood pressure and arteriosclerosis.

Here, the olive extract is preferably extracted from an olive fruit, but is not limited thereto.

Sandalwood is the heartwood of dandelion and tree trunks. It has a clear scent and has a calming effect to relieve tension and anxiety.

And, sandalwood has sedative and anti-inflammatory properties, so it increases immunity against coughs and sore throats, prevents infection, relieves symptoms, and promotes sleep.

Aloesaceae is an evergreen tree in the Azalea family, and it helps blood circulation, and contains delta guaien, which activates brain neurotransmitters and helps brain health.

Here, based on 100% by weight of the total eucalyptus powder 0.1% to 0.3% by weight, Daecheong root extract 0.001% to 0.005% by weight, olive extract 0.005% to 0.01% by weight, sandalwood extract 0.0005% by weight to 0.001% by weight, 0.001% to 0.003% by weight of aloes powder is added.

In addition, lavender powder and lemon powder may be further added.

Lavender regulates stress by harmonizing the nervous system, promotes vitality, strengthens nerves, and has a calming effect.

In addition, lavender has a great effect on insomnia, especially insomnia caused by mental stress and anxiety, prevents infection, has antiseptic, analgesic, and cell regeneration promoting properties that help rapid healing, colds and influenza, bronchitis, sore throat, It can also be applied to the treatment of catarrhal symptoms.

Lemon is rich in liminoid, an antioxidant component, which helps to suppress free radicals and aging, and helps immunity, so it is often applied to detoxification products.

Here, 0.015 wt% to 0.08 wt% of lavender powder and 0.001 wt% to 0.01 wt% of lemon powder are added based on 100 wt% of the total.

As described above, a plant-extracted antibacterial composition can be prepared by adding a cypress extract, a moss extract, and a pogongyeong extract to ethanol in a mixing tank, and then adding and mixing auxiliary materials and/or additives.

Here, when the mixture to be added and added into the mixing tank is stirred and mixed, it may be mixed at a temperature range of 60°C to 90°C for 1 hour to 3 hours.

Then, the mixed solution is sterilized by heating to a temperature of 95° C. or higher for 15 to 30 minutes, and cooled at room temperature.

After storing the plant-extracted antibacterial composition prepared in this way in the storage container of the aerosol-type spraying device, it is sprayed to the outside through the spray nozzle provided in the spraying device, and various bacteria, harmful pathogens and bacteria It can prevent the reproduction and transmission of children.

In addition, a large amount of phytoncide is generated, and negative ions are discharged to purify the air, and since ingredients extracted from natural plants are used as the main raw material, it is not only harmless to the human body, but also can be used for companion animals.

Experimental Example 1: Sterilization power test of antibacterial composition

The sterilization power of the plant-extracted antibacterial composition according to this example was tested. At this time, as the test strains, Staphylococcus aureus ATCC 6538, Staphylococcus aureus ATCC 33591), Escherichia coli ATCC 8739, Klebsiella pneumoniae ATCC 4352, Salmonella typhimurium KCTC 1925 pseudomonas aeruginosa ATCC 27853) was used.

After culturing each test strain in a liquid medium (TRYPTIC SOY AGAR), the cultured bacteria were diluted and the initial inoculation strain was adjusted to be 1 × 10 ⁵ CFU/mL to 9 × 10 ⁵ CFU/mL and used.

Put 20 mL of the plant-extracted antibacterial composition according to this example in a sterilized cap tube, adjust the initial inoculation strain by diluting each cultured bacteria, and measure the number of bacteria 5 minutes after inoculation at room temperature to reduce the initial number of bacteria was measured.

However, in the first dilution step, the experiment was conducted through the neutralization process using the D/E NEUTRALIZING BROTH (DIFCO) neutralization method.

When bacteria proliferated in the medium, the value was calculated by multiplying the dilution factor by the strain on the medium. ” is indicated.

At this time, the reduction rate (99.9% or more) of the strain measured after 5 minutes was calculated from the relational expression of {(A-B)/A} x 100. A is the initial inoculation strain, and B is the strain measured after 5 minutes.

As a result of the experiment, as shown in FIG. 2, when the plant extract antibacterial composition according to this example was exposed for 5 minutes, all of the test strains did not proliferate, and accordingly, as shown in FIGS. 3 to 8, this practice It can be seen that the plant-extracted antibacterial composition according to the example is inhibited by more than 99.9% against bacteria and pathogenic harmful bacteria, thereby confirming that it has excellent sterilization power.

Experimental Example 2: Deodorization test of antibacterial composition

A plant-extracted antibacterial composition according to this embodiment, which is a gas with strong odor components such as toilet smell, sweat smell, food smell, etc. Deodorization was tested.

As a result of the experiment, as shown in FIG. 9 , in the case of the plant-extracted antibacterial composition according to this example, it was confirmed that the inhibitory ability of 99.6% and 99.7% with respect to ammonia and acetic acid 30 minutes after mixing with ammonia and acetic acid after 30 minutes had elapsed. could

Examples and Comparative Examples

Hereinafter, the present invention will be specifically described with reference to Examples and Comparative Examples. An average person skilled in the art to which the present invention pertains can change the present invention in various other forms other than the composition described in the examples below, and the following examples only illustrate the present invention, and the scope of the technical spirit of the present invention is limited to those of the following examples. It should not be construed as intended to limit the scope.

Example 1

In the mixing tank, 825ml of ethanol, 100ml of cypress extract, 50ml of moss extract and 25ml of pogongyeong extract are added and mixed, and after mixing at 75℃ for 3 hours, the mixed solution is sterilized by heating at a temperature of 97℃ or higher for 15 minutes. treatment and cooling at room temperature to prepare a plant extract antibacterial composition.

The prepared plant extract antibacterial composition was used by storing it in the storage container of the sprayer.

Example 2

A plant-extracted antibacterial composition was prepared in the same manner as in Example 1, except that 500 ml of ethanol, 300 ml of cypress extract, 100 ml of moss extract, and 100 ml of pogongyeong extract were added in the mixing tank.

Example 3

A plant-extracted antibacterial composition was prepared in the same manner as in Example 1, except that 910 ml of ethanol, 50 ml of cypress extract, 30 ml of moss extract, and 10 ml of pogongyeong extract were added in the mixing tank.

Example 4

A plant-extracted antibacterial composition was prepared in the same manner as in Example 1, except that 300 ml of ethanol, 350 ml of cypress extract, 200 ml of moss extract, and 150 ml of pogongyeong extract were added in the mixing tank.

Example 5

A plant-extracted antibacterial composition was prepared in the same manner as in Example 1, except that 950 ml of ethanol, 30 ml of cypress extract, 15 ml of moss extract, and 5 ml of pogongyeong extract were added in the mixing tank.

Comparative Example 1

Commercially available "cypress water phytoncide undiluted solution" was put into a spray spray device and used.

Comparative Example 2

Commercially available "cypress spray leaf sap phytoncide" was put into a spray spray device and used.

division scent preference stimulus degree refreshing vitality overall sign Example 1 4.7 4.5 4.8 4.7 4.7 Example 2 4.5 4.7 4.6 4.6 4.5 Example 3 4.5 4.3 4.4 4.4 4.4 Example 4 4.4 4.8 4.5 4.5 4.4 Example 5 4.3 4.2 4.3 4.3 4.3 Comparative Example 1 4.3 4.4 4.2 4 4.1 Comparative Example 2 4.3 4.3 4.1 3.9 4.1

As shown in [Table 1], after 30 minutes after spraying the plant-extracted antibacterial composition prepared in Examples 1 to 5 and the phytoncide antibacterial product according to Comparative Examples 1 and 2 to bedding 3 times in 10-minute increments The test was conducted for 30 adults in their 20s to 60s, and the score and evaluation criteria were performed on a 5-point scale.

As a result of the experiment, it was confirmed that the plant-extracted antibacterial composition prepared in Examples 1 to 3 was superior to the plant-extracted antibacterial composition prepared in Examples 4 and 5 in flavor preference, irritation, freshness, durability, and overall preference. , In the case of Examples 2 and 4, it can be seen that the cypress extract, the moss extract and the pogongyeong extract are contained in a relatively high degree of fragrance, and in Examples 3 and 5, the cypress extract, the moss extract and the pogongyeong extract It can be seen that because it contains a small amount, the fragrance is relatively mild, and the freshness and durability are reduced.

In addition, it was confirmed that the fragrance preference, stimulation degree, freshness, lasting power, and overall preference were superior to those of Comparative Examples 1 and 2 compared to Examples 1 to 5. It is judged that, in addition to the cypress extract, the moss extract and the pogongyeong extract are included to satisfy the consumer's preference.

Therefore, it was confirmed that the plant-extracted antibacterial composition according to the present invention contains a moss extract and a pogongyeong extract in addition to the cypress extract, so that the phytoncide content is superior to that of the existing product, and thus it has a high marketability compared to the existing product.

As mentioned above, although the present invention has been shown and described in relation to specific embodiments, it is common knowledge in the art that various modifications and changes are possible without departing from the spirit and scope of the invention as set forth in the appended claims. Anyone who has it will know it easily.

Claims (6)

obtaining a cypress extract, a moss extract, and a pogongyeong extract from cypress trees and moss plants and pogongyeong (S10);
A step of vacuum low-temperature extraction of each of the obtained cypress extract, moss extract, and pogongyeong extract (S30);
Cooling the vacuum low temperature extracted cypress extract, moss extract and pogongyeong extract (S50);
distilling the cooled cypress extract, moss extract, and pogongyeong extract (S70); and
After adding water or ethanol into the mixing tank, the distilled cypress extract, moss extract, and pogongyeong extract are added and mixed (S90);
A method for producing an antibacterial composition comprising a plant extract as an active ingredient, comprising:
The method according to claim 1,
vacuum low-temperature extraction of the obtained cypress extract, moss extract, and pogongyeong extract with water or alcohol at a weight ratio of 1:1 to 20, respectively;
Method for producing an antibacterial composition comprising a plant extract as an active ingredient, characterized in that it further comprises.
The method according to claim 1,
Based on 100% by weight of the total in the mixing tank, 5% to 30% by weight of the cypress extract obtained in 50% to 85% by weight of water or ethanol, 3% to 10% by weight of the moss extract obtained, and obtained A method for producing an antibacterial composition comprising a plant extract as an active ingredient, characterized in that 1% to 10% by weight of the pogongyeong extract is added and mixed.
The method according to claim 1,
When adding water or ethanol, cypress extract, moss extract, and pogongyeong extract to the mixing tank, additional materials are further added, wherein the auxiliary material is a water-soluble silicate. A method for producing an antibacterial composition comprising a plant extract as an active ingredient.
5. The method of claim 4,
When water or ethanol, cypress extract, moss extract, pogongyeong extract, and auxiliary materials are added in the mixing tank, additives are further added, and as the additive, any one or more of jasmine powder, peppermint powder, or pine needle powder is further added. A method for producing an antibacterial composition comprising a plant extract as an active ingredient.
A plant extract antibacterial composition comprising the plant extract prepared by the method of any one of claims 1 to 5 as an active ingredient.

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