KR100285781B1 - Essential oil composition with antibacterial and antifungal action - Google Patents

Abstract

The present invention relates to a composition having an antibacterial and antifungal action composed of various essential oils, and more particularly, oregano, manuka, dill, lavender, lemon balm. (Lemon balm), Rosemary, Mint, Savory, Sage, Scentedgeranium, Self hill, Sweet Basil, Chervil One or more of essential oils consisting of Thyme, Tansy, Cinnamon, Clove, Eucalyptus, Lemon, Peppermint and Tea tree The composition of the present invention made of a combination of the components, environmentally friendly antimicrobial that has a broader and better antibacterial, antifungal and bactericidal action than essential oils alone, and is composed of natural ingredients that are harmless to the human body, leaving no residual toxicity and It can be usefully used as an antifungal agent. In addition, the composition of the present invention can be used as a fragrance as well as aromatherapy due to the unique flavor and pharmacological action of each component.

Description

Essential oil composition with antibacterial and antifungal action

The present invention relates to a composition having an antibacterial and antifungal action composed of various essential oils, and more specifically, contains oregano and / or Manuka, which are particularly excellent in antibacterial and antifungal action. In addition to these ingredients, Dill, Lavender, Lemon Balm, Rosemary, Mint, Savory, Sage, Scented-geranium, Self hill, Sweet Basil, Chervil, Thyme, Tansy, Cinnamon, Clove, Eucalyptus, Lemon, The present invention relates to a composition formed by selecting and combining one or more components from essential oils composed of peppermint and tea tree. The composition of the present invention can be used not only as a fragrance but also applied to fragrance therapy.

The antimicrobial agent prevents the production of microorganisms or suppresses the reproduction of industrial antimicrobial agents currently developed about 130 species, in consideration of human safety aspects, it is preferable to use a natural antimicrobial agent extracted from natural organisms.

Natural antimicrobial is a substance that suppresses microbial contamination and growth, and is widely used as food additives, pharmaceuticals, and industrial substances. -100 and the like have been developed, but the scale is only 17% of the synthetic preservatives.

Herbs are edible, medicinal, and fragrances. These are the generic term for fragrant plants that grow on the Mediterranean coast and Southwest Asia, and recently include the leaves, flowers, and fruits of trees. Essential oil extracted from such herbs is a volatile oil having a unique aroma, also called aroma oil (芳香 油). In particular, there are more than 500 varieties of herb extracts, anise oil, citronella oil, terepin oil, peppermint oil, rosemary oil, etc., and about 100 of them are currently used as raw materials for edible, medicinal and natural flavors.

Essential oils each have a unique scent and have strong antiseptic and antiseptic properties. Essential oils are chemically divided into oxygen-containing compounds such as alcohol aldehydes, ketones, organic acids, esters, and mono and sesquiterpene hydrocarbons. The former is involved in the aroma and taste of essential oils. It is difficult to dissolve in water and is rather an obstacle to aroma or taste.

Essential oils, herbal extracts, have the unique properties and ingredients of natural products, and have been used in various ways until now. In other words, it is used as a therapeutic tool, a spice, a digestive agent, a neuro stabilizer, and an odor inhibitor, as well as a mental healing tool to relax skin and to relax the mind and body, and to clear the head.

The present inventors found that herbal extracts strongly inhibit the growth of various bacteria, fungi and yeasts, and searched for more specific antimicrobial and antifungal activity, as a composition with other herbal extracts than when using herbal extracts alone. The present invention has been completed by finding out that it can be usefully used in fragrances and aromatherapy as well as exhibiting a wider and surprising antimicrobial and antifungal effect.

An object of the present invention is to provide a composition having excellent antimicrobial and antifungal effects comprising a combination of various essential oils, including oregano or manuka.

The present invention also provides a use of the composition as a perfume.

The present invention also provides a use of the composition in aromatherapy.

1 is oregano (Oregano) and will showing a result of searching the antimicrobial activity compared to the bacteria Bacillus cereus (Bacillus cereus) 1 according to the dilution ratio of the tea extract of Manuka (Manuka),

(M: Manuka, O: Oregano)

(O, M: Stock: 10 ⁰ , 1: 10 ^-1 , 2: 10 ^-2 , 3: 10 ^-3 , 4: 10 ^-4 , 5: 10 ^-5 , 6: 10 ^-6 )

(50P: 50 μl drop of sample)

Figure 2 shows the results of comparing the antimicrobial activity against the fungus Aspergillus niger according to the dilution ratio between the oregano and Manuka primary extract.

(M: Manuka, O: Oregano)

(O, M: Stock: 10 ⁰ , 1: 10 ^-1 , 2: 10 ^-2 , 3: 10 ^-3 , 4: 10 ^-4 , 5: 10 ^-5 , 6: 10 ^-6 )

(50P: 50 μl drop of sample)

In order to achieve the above object, the present invention contains oregano and / or manuka, which is particularly excellent in antibacterial and antifungal action, and in addition to these ingredients, dill, lavender, lemon balm, rosemary, mint, sage, sage, cented geranium, self heel It provides a composition consisting of selecting and combining one or more ingredients from essential oils consisting of sweet basil, chervil, thyme, tanji, cinnamon, clove, eucalyptus, lemon, peppermint and tea tree.

Hereinafter, the present invention will be described in detail.

First, herbal extracts, ie essential oils, which may be components of the composition of the present invention are as follows;

Dill; Anethum graveolens , Lavender; Lavandula spica , Lemon balm; Melissa officinalis , Rosemary; Rosmarinus officinalis , Mint; Mentha spp. , Savory; Satureia hortensis , Sage; Salvia officinalis , Scentedgeranium; Pelargonium spp. , Self hill; Prunella vulgaris , Sweet Basil; Ocimum basilicum , Oregano; Origanum vulgare Chervil; Antbriscus cerefolium ), Thyme; Thymus vulgaris , Tanji (Tansy; Tanacetum vulgare ), Cinnamon; Cinnamonum ceylanicum , Colov (Clove; Eugenia caryophyllata ), Eucalyptus (Eucalyptus; Eucalytus spp ) Lemon; Citrus limonum ), Manuka ( Leptospermium scoparium ), Peppermint ( Mentha piperita ) and Tea tree ( Melaleuca alternifolia ).

Dill grows more than one meter in the early years of the Araaceae family, native to the Mediterranean coast, West Asia, India, and Iran. Dill has aromas throughout the plant, making it possible to use flowers, leaves, roots and seeds. It is suitable for cooking of any ingredients, especially since leaf essential oils remove fishy smells.

Lavender is the best-known variety of herbs and is a perennial plant of evergreen grassland with more than 28 varieties. The Mediterranean coast is native and grows up to 0.15 to 1 meters. In particular, the entire plant, such as flowers, leaves, stems, fragrance, as well as ornamental, potpourri and various beauty materials, lavender essential oil is also used as a sedative in aromatherapy. In addition, there is a sterilization and disinfection effect can be applied to light wounds and insect bites to treat trauma.

Lemon balm, also known as Melissa, is native to the Mediterranean coast and is native to eastern Mediterranean, West Asia, the Black Sea and central Europe. It is a perennial plant of Lamiaceae. White and small flowers grow on petioles in early summer, and shiny green stems grow up to 1 meter long and can smell strong lemon. When bitten by scorpions or poisonous spiders, they have detoxification effects and are effective in relieving diarrhea and preventing viruses, and are effective in smallpox.

Rosemary is a perennial evergreen plant from Lamiaceae, widely distributed along the Mediterranean coast, growing up to 2 meters long and used as a herb from the first century BC. Rosemary has a unique strong aroma to use all stems, leaves, and flowers, and is used in carnivorous dishes because the fragrance is preserved even when heated, and the components emitted from flowers or leaves have antibacterial and disinfectant effects.

The ivory belongs to the Lamiaceae family and includes perennial winter ivory and annual summer ivory. The odor of winter savory is stronger and is called peppermint. Winter saber and summer saber both promote digestion and disinfection, and are effective at blowing into bees. Tea or leach solution made of ivory is also effective for killing phlegm, stroke, and diuresis, as well as insect repellent and antiseptic effect.

Sage is a variety of varieties and can be used anywhere, is known as an all-round herb, especially used in ornamentals, flavoring, harvesting, medicine, cooking, dyeing. It has tonic, soothing, digestion and sterilization effects. It is very important for cooking because it neutralizes meat and fish fat to remove odor.

Sented geranium is a perennial herb with red pepper sprouts and is native to South Africa and is widely distributed in the West and Asia. It is mainly grown in South France and Africa as a raw material for perfumes, and is a component of men's perfumes, and is popular in Europe for ornamental potted plants.

Self Hill is an evergreen perennial plant with nectar originating from Europe to Asia. Since ancient times, self-heels have been used for folk medicine, and buds or stems of flowers that start to bloom in early summer were used as a lip repellent medicine, and diuretics were made from fully dried buds, and the wounds were treated with leaves. Leachate is effective for stomatitis and tonsillitis and is used as a diuretic for nephritis and cystitis.

Sweet basil is native to India and has many varieties with Lamiaceae. It is widely used in cooking as it is called a kitchen herb. The appetizing scent is a lot of tea to drink, especially for those with a weak stomach because it has an appetite boosting and digestion promoting effect.

It is native to the Middle East of Europe and is an evergreen of 30-60 cm long, belonging to the Buttercup family. Cavill juice has pain relief and anti-inflammatory properties, which can be used as a bath or poultice to help heal wounds and inflammation. As it is applied to insect bites and cleans the skin, it is effective for cleaning rough skin, preventing hair loss, and preventing wrinkles.

Thyme is an evergreen small tree with Lamiaceae, native to the Mediterranean, whose stems grow to 15 to 30 centimeters. Thymol, the main ingredient, has a strong bactericidal power. It is used for rehabilitation of the mouth and skin, and it is used as a medicine for skin diseases. It is effective for preventing hair loss and mental tonic.

Tangji is an evergreen perennial herb of the Asteraceae family, which is native to England and Northern Europe. It is also famous as a dyed herb, and its dry leaves have insecticidal and bactericidal effects.

Oil extracted from the leaves of eucalyptus trees is a powerful natural preservative and can be used to treat a wide range of bacteria and viruses. It also helps to relieve dyspnea caused by asthma, and it is effective in treating bronchitis by removing sputum, and it can be used as a home fungicide.

Peppermint has excellent bactericidal and antiparasitic effects among mints. The refreshing scent is used for toothpaste because it prevents bad breath. Grind the leaves and use it as a poultice to treat the inflammation or bruise of the skin, and it is also effective for dermatitis and itching.

Tea tree has strong antiseptic and antibacterial properties, so it is effective for various skin diseases such as acne. It is not toxic and has no side effects such as allergies, and is useful for various respiratory diseases such as eucalyptus.

Oregano is a perennial herb that is native to Europe and Southwest Asia, and is a viable species of marjoram. It is the same genus as sweet marjoram, but has a better aroma and taste. The stem is 30 to 90 centimeters long and the roots spread horizontally, so the stem grows like the ground. It also has antiseptic and detoxification effects, so it's known as an antidote when bitten by snakes or scorpions.

Manuka is a natural oil native to New Zealand. It is soft and versatile, like lavender, and preservative like tea tree oil. It is good for healing skin such as eczema wound and has strong antibacterial effect, which shows good healing effect for thrush and vaginitis.

I) 0.1 to 99.9 weight percent of oregano; And ii) dill, lavender, lemon balm, rosemary, mint, savory, sage, cented geranium, self heel, sweet basil, chervil, thyme, tanji, cinnamon, clove, eucalyptus, lemon, peppermint and tea tree Provided are antimicrobial and antifungal compositions comprising from 0 to 99.9% by weight of one or more essential oils.

Of these compositions, in particular, a composition consisting of 0 to 80% by weight of one or more essential oils selected from i) 0 to 80% by weight of oregano, ii) lavender, lemon balm, thyme, eucalyptus, lemon and tea tree is preferred.

In the present invention, i) 0.1 to 99.9% by weight of Manuka; And ii) dill, lavender, lemon balm, rosemary, mint, savory, sage, cented geranium, self heel, sweet basil, chervil, thyme, tanji, cinnamon, clove, eucalyptus, lemon, peppermint and tea tree Provided are antimicrobial and antifungal compositions comprising from 0 to 99.9% by weight of one or more essential oils.

Of these compositions, in particular, a composition consisting of 0 to 80% by weight of one or more essential oils selected from i) 0 to 80% by weight of manuka, ii) lavender, lemon balm, thyme, eucalyptus, lemon and tea tree is preferred.

In the present invention, i) 0.1 to 99.9% by weight of oregano, 0.1 to 99.9% by weight of Manuka; And ii) dill, lavender, lemon balm, rosemary, mint, savory, sage, cented geranium, self heel, sweet basil, chervil, thyme, tanji, cinnamon, clove, eucalyptus, lemon, peppermint and tea tree Provided are antimicrobial and antifungal compositions comprising from 0 to 99.9% by weight of one or more essential oils.

Among these compositions, i) 0-80% by weight of oregano, 0-80% by weight of Manuka, ii) 0-70% by weight of one or more essential oils selected from lavender, lemon balm, thyme, eucalyptus, lemon and tea tree This is preferred.

The composition of the present invention contains oregano and / or manuka, which are particularly excellent in antibacterial and antifungal activity, as a main component, and is a composition composed of various other essential oils, and exhibits stronger antibacterial and antifungal effects than when each essential oil is used alone. (See experimental example below).

The composition of the present invention, as each component has a unique natural fragrance, can be used as a fragrance as well as aroma therapy using the pharmacological action of each component.

On the other hand, the composition of the present invention can be used by dissolving in a solvent, sol ethanol, isoparaffin or a mixed solution thereof may be used as a solvent that can be used at this time.

Hereinafter, the present invention will be described in more detail with reference to Examples and Experimental Examples. However, the following Examples and Experimental Examples are intended to illustrate the present invention, but the present invention is not limited thereto.

The composition of the present invention was prepared in the same composition as in the following examples.

<Example 1>

Oregano ------------------ 69.4 wt%

Lemon ---------------------- 5.3 wt%

Eucalyptus ---------------- 9.8 wt%

Lavender -------------------- 15.5 wt%

<Example 2>

Oregano ------------------ 70.2 wt%

Lemon ---------------------- 4.8 wt%

Tea Tree ------------------ 10.8 wt%

Peppermint ------------------ 14.2 wt%

<Example 3>

Oregano ------------------ 75.7 wt%

Lemon Balm ------------------- 9.5 wt%

Time --------------------- 14.8. weight%

<Example 4>

Manuka -------------------- 69.4% by weight

Lemon ---------------------- 5.3 wt%

Eucalyptus ---------------- 9.8 wt%

Lavender -------------------- 15.5 wt%

<Example 5>

Manuka ------------------------ 50.1 wt%

Rosemary ---------------------- 8.7 wt%

Eucalyptus -------------------- 20.3 wt%

Mint -------------------------- 4.7 wt%

Tangier -------------------------- 16.2 wt%

<Example 6>

Manuka ---------------------- 90.1 wt%

Cinnamon ---------------------- 2.5% by weight

Peppermint -------------------- 3.8 wt%

Tea Tree --------------------- 3.6% by weight

<Example 7>

Oregano ------------------ 60.4 wt%

Manuka -------------------- 20.1 wt%

Lemon ---------------------- 2.2 wt%

Lavender -------------------- 17.3 wt%

<Example 8>

Oregano ------------------ 40.1 wt%

Manuka -------------------- 40.1 wt%

Eucalyptus ---------------- 9.8 wt%

Ivory ------------------- 0.3 wt%

Self Heel ------------------- 2.3 wt%

Cavill ---------------------- 7.4 wt%

<Example 9>

Oregano ------------------ 20.5 wt%

Manuka -------------------- 40.6 wt%

Sweet basil --------------- 11.2 wt%

Lavender -------------------- 9.7 wt%

Cinnamon -------------------- 18.0 wt%

<Example 10>

Oregano ------------------ 33.2 wt%

Manuka -------------------- 37.9 wt%

Seibori ------------------- 12.1 wt%

Sage -------------------- 3.1 wt%

Lavender -------------------- 13.7 wt%

Experimental Example 1 Determination of Antimicrobial Activity of Herbal Extract

Experimental Sampleas Herbs native to New Zealand, Mexico, Australia, Europe, and Korea and known to have antimicrobial activity were used to detect the antimicrobial activity of each of the extracts.

Test strainAs Actinobacillus urea, a dominant species bacteria related to corruptionActinobacillus ureae), Bacillus cereus (Bacillus cereus), Bacillus succulence (Bacillus circulans), Bacillus Megaterium (Bacillus megaterium), Bacillus subtilis (Bacillus subtilis), Bacillus Surgeoniens (Bacillus thuringiensis), Candida Albicans (Candida albicans), Enterobacter Aerogenes (Enterobacter aerogenes), Escherichia coli (Escherichia coli), Listeria monobitogen (Listeria monovytogenes), Salmonella Enteritidis (Salmonella enteritidis), Salmonella typhimurium (Salmonella typhimurium), Pseudomonas eruzinosa (Pseudomonas aeruginosa), Pseudomonas aeruginosa-1 (Pseudomonas aeruginosa-1), Staphylococcus auricularis (Staphylococcus auricularis), Staphylococcus aureus MRSA (Staphylococcus aureus MRSA), Staphylococcus aureus MSSA (Staphylococcus aureus MSSA) And Streptococcus mutans (Streptococcus mutans18 kinds) were used.

Used badgeIs LB agar was used to measure the antimicrobial activity against bacteria, and the antimicrobial activity against the fungus was 1.9 ml /% of 10% tartaric acid in PDA medium (from 200 g of potato, 20 g of dextrose, 15 g of agar). The mixture was used at a rate of 100 ml.

In order to measure the antimicrobial activity, the antimicrobial activity assay method for bacteria was prepared by sterilizing LB liquid nutrient medium, taking each strain 1 platinum, and incubating in a shaking incubator at 37 ° C. for 48 hours.

1.5% of agar was added to the LB liquid nutrient medium as described above, prepared by pouring into a petri dish to prepare a basal medium, and cultured when the medium containing 0.75% of agar in the LB liquid nutrient medium became 45 to 50 ° C. Mix the strain well and pour over the substrate medium to make a medium medium.

Samples were placed on a paper disc in a fixed amount and placed on a medium medium and incubated for 48 hours in a 37 ℃ incubator. Table 1 shows the results of measuring the diameter of the clear zone (clear zone) appeared after the strain was grown.

Antimicrobial Activity of Herb Extracts against Various Bacteria Isolated from Nature number Sample name Bacillus surging gensis Bacillus cereus Bacillus Megaterium Staphylococcus aulicularis One Dill 39 33 25 17 2 lavender 40 37 25 20 3 Lemon balm 45 40 30 28 4 Rosemary 28 13 15 10 5 Mint 40 37 25 20 6 Seyborg 25 30 29 15 7 sage 37 25 20 15 8 Scented Geranium 40 37 25 20 9 Self heel 15 14 15 13 10 Sweet basil 39 33 25 17 11 oregano 54 48 36 40 12 chervil 39 33 25 17 13 time 25 20 15 15 14 Tanji 20 15 15 14 15 Cinnamon 39 33 25 17 16 Clove 40 37 25 20 17 Eucalyptus 39 33 25 17 18 lemon 40 37 25 20 19 Manuka 45 40 30 28 20 Peppermint 15 13 10 10 21 Tea tree 12 15 13 10 Unit: mm (Clean Area Diameter)

number Sample name Actinobacillus Urea Bacillus succulence Pseudomonas Eruzinosa Pseudomonas aeruginosa-1 Staphylococcus aureus MRSA One Dill 11 19 11 12 14 2 lavender 15 15 14 15 13 3 Lemon balm 13 15 12 17 16 4 Rosemary 10 11 11 12 19 5 Mint 15 25 30 15 13 6 Seyborg 13 15 12 15 14 7 sage 11 10 10 11 10 8 Scented Geranium 15 15 14 12 13 9 Self heel 13 15 12 10 18 10 Sweet basil 11 11 12 15 13 11 oregano 25 30 29 31 29 12 chervil 20 11 11 12 11 13 time 14 15 14 15 13 14 Tanji 15 15 12 15 14 15 Cinnamon 10 13 12 12 15 16 Clove 15 15 14 15 13 17 Eucalyptus 11 11 21 12 11 18 lemon 15 15 14 15 13 19 Manuka 13 15 12 15 12 20 Peppermint 10 10 11 11 12 21 Tea tree 11 11 12 15 13 Unit: mm (Clean Area Diameter)

number Sample name Staphylococcus aureus MSSA Bacillus subtilis Enterobacter Aerogenes Escherichia coli Streptococcus mutans One Dill 11 25 12 15 19 2 lavender 13 20 20 13 11 3 Lemon balm 14 15 14 13 15 4 Rosemary 11 15 10 10 11 5 Mint 13 25 20 15 25 6 Seyborg 15 25 15 13 15 7 sage 12 28 15 11 10 8 Scented Geranium 13 40 20 15 15 9 Self heel 14 15 13 13 15 10 Sweet basil 10 25 11 11 11 11 oregano 29 30 20 28 18 12 chervil 11 29 17 20 11 13 time 13 25 15 14 15 14 Tanji 14 20 14 15 15 15 Cinnamon 11 19 11 10 13 16 Clove 13 15 15 15 15 17 Eucalyptus 10 25 11 11 11 18 lemon 13 20 12 15 15 19 Manuka 14 24 20 13 15 20 Peppermint 10 15 10 10 10 21 Tea tree 10 12 10 11 11 Unit: mm (Clean Area Diameter)

number Sample name Candida Albicans Listeria monovitogen Salmonella typhimurium Salmonella Enteritidis One Dill 15 12 14 13 2 lavender 15 13 11 11 3 Lemon balm 12 17 16 14 4 Rosemary 11 12 11 11 5 Mint 23 15 13 13 6 Seyborg 12 15 14 15 7 sage 10 11 10 12 8 Scented Geranium 14 12 13 13 9 Self heel 12 10 18 14 10 Sweet basil 12 15 13 10 11 oregano 18 20 17 15 12 chervil 11 12 11 11 13 time 14 15 13 13 14 Tanji 12 15 14 14 15 Cinnamon 12 12 15 11 16 Clove 14 15 13 13 17 Eucalyptus 21 12 11 10 18 lemon 14 15 13 13 19 Manuka 12 15 12 14 20 Peppermint 11 11 12 10 21 Tea tree 12 15 13 10 Unit: mm (Clean Area Diameter)

Experimental Example 2 Antifungal Activity of Herbal Extracts

Experimental Sampleas Herbs native to New Zealand, Mexico, Australia, Europe and Korea and known to have antifungal activity were used to search for antifungal activity against fungi and yeast in each of the extracts.

Blood MoldAs for Altenaria Altenata (Alternaria alternata), Aspergillus Niger (Aspergillus niger), Aspergillus Oraijae (Aspergillus oryzae), Muco Musedo (Mucor mucedo), Muco Roissy (Mucor rouxii), Neurospora cytophiles (Neurospora sitophila), Penicillium Notatum (Penicillium notatum), Pichia Membra Napaciens (Pichia membranaefaciens), Lycopus Delimer (Rhizopus delimer), Tanidium Elegance (Thamnidium elegans), TricothermaTricoderma viridae), Zaigo Saccharomyces Salus (Zygosaccharomyces salus) And Zaigo Saccharomyces japonicus (Zygosaccharomyces japonicus)of 13 types were used.

In order to measure the antifungal activity, the antifungal force measurement method for the fungus was prepared by disinfecting the PDA medium into a Petri dish, incubating the base medium, inoculating each mold on the base medium, and incubating for 72 hours in a 30 ° C. incubator.

Sterilize 0.01% Tween 80 and place in an amount in a Falcon tube and prepare by suspending each mold. 1.5% agar was added to the PDA medium and sterilized. When the medium became 45 ° C, 1.9 ml / 100 ml of 10% tartaric acid was added, and the prepared mold suspension was added at a rate of 1 ml / 15 ml. After dropping a predetermined amount on a medium and incubated at 30 ℃ for 72 hours to measure the diameter of the clean zone appeared after the strain was grown, the results are shown in Table 2.

Antifungal Activity of Herbs against Various Fungi Isolated from Nature number Sample name Altenaria Altenata Aspergillus Niger Aspergillus Oraijae Zaigo Saccharomyces Salus Zaigo Saccharomyces japonicus One Dill 10 12 10 11 20 2 lavender 15 13 15 14 14 3 Lemon balm 15 14 15 14 13 4 Rosemary 11 19 10 11 11 5 Mint 25 13 15 13 9 6 Seyborg 15 14 13 15 10 7 sage 10 10 11 20 20 8 Scented Geranium 15 13 15 18 15 9 Self heel 15 18 13 11 12 10 Sweet basil 11 13 11 10 13 11 oregano 54 20 35 15 8 12 chervil 15 16 13 14 11 13 time 15 13 14 11 10 14 Tanji 15 14 15 10 20 15 Cinnamon 13 15 10 9 13 16 Clove 15 13 15 8 17 17 Eucalyptus 17 18 18 15 14 18 lemon 15 20 15 17 11 19 Manuka 29 12 20 13 13 20 Peppermint 19 14 11 25 17 21 Tea tree 11 13 11 21 12 Unit: mm (Clean Zone Diameter)

number Sample name Muco Musedo Muco Roissy Neurospora Cytopilia Pichia Membra Napaenance One Dill 10 11 15 10 2 lavender 13 14 40 20 3 Lemon balm 14 12 20 14 4 Rosemary 11 11 28 15 5 Mint 13 30 40 15 6 Seyborg 15 12 25 20 7 sage 12 10 37 13 8 Scented Geranium 13 14 40 17 9 Self heel 14 12 15 20 10 Sweet basil 10 12 39 11 11 oregano 18 29 50 15 12 chervil 14 12 45 10 13 time 13 14 25 12 14 Tanji 14 12 20 17 15 Cinnamon 11 12 39 15 16 Clove 13 14 40 16 17 Eucalyptus 25 25 43 13 18 lemon 29 30 40 26 19 Manuka 12 15 40 13 20 Peppermint 11 11 39 15 21 Tea tree 10 12 12 21 Unit: mm (Clean Zone Diameter)

number Sample name Penicillium Notatum Raizofus Delimer Tanidium Elegance Tricorderma Squadron One Dill 10 10 13 11 2 lavender 20 25 37 15 3 Lemon balm 14 15 19 15 4 Rosemary 10 15 13 12 5 Mint 20 25 37 15 6 Seyborg 15 29 30 15 7 sage 15 20 25 11 8 Scented Geranium 20 25 37 12 9 Self heel 13 15 14 10 10 Sweet basil 17 25 33 15 11 oregano 20 60 55 40 12 chervil 28 30 40 17 13 time 15 15 20 15 14 Tanji 14 15 15 15 15 Cinnamon 17 25 33 12 16 Clove 20 25 37 15 17 Eucalyptus 20 30 40 16 18 lemon 20 25 37 13 19 Manuka 13 30 15 20 20 Peppermint 17 25 33 12 21 Tea tree 10 13 15 15 Unit: mm (Clean Zone Diameter)

Experimental Example 3 Antibacterial Activity of Diluent

Minimal Inhibitory Concentration screening was performed by comparing the antimicrobial activity of each sample diluted from 10 ⁰ (stock solution) to 10 ^-6 .

As a result of the test, the primary extract of Manuka showed antimicrobial activity up to 1000-fold diluted samples, whereas cypress oil and the like showed antimicrobial activity only in the stock solution. In addition, the first extract of Manuka had antimicrobial activity up to 1000 times diluted to bacteria as in the previous experiments, oregano samples showed antimicrobial activity in samples diluted to 10 ^-6 to confirm the excellent antimicrobial activity of oregano ( See Table 3 and FIG. 1 ).

Experimental Example 4 Antifungal Activity of Diluent

The minimum inhibitory concentration search for the fungus was carried out by diluting from 10 ⁰ (stock solution) to 10 ^-6 with four types of antimicrobial activity in Test Example 3, and comparing the antifungal powers to the three types of fungi.

As a result of the test, the primary extract of Manuka had antifungal activity up to 1000 times diluted in fungus as in bacteria, and oregano also showed strong antifungal power in samples diluted to ^10-6 (see Table 4 and FIG. 2 ).

Experimental Example 5 Antimicrobial and Antifungal Activity of the Composition

First composition; 60% oregano and 40% lemon

Second composition; Oregano 60% and Eucalyptus 40%

Third composition; 60% oregano and 40% lavender

Fourth composition; Oregano 60% and Tea Tree 40%

Tests were carried out in the same manner as in Experimental Example 1 and Experimental Example 2 using the composition to obtain the results of Tables 5 and 6.

Antibacterial test First composition Second composition Third composition Fourth composition Bacillus surging gensis 58 57 55 55 Bacillus cereus 52 50 49 48 Bacillus Megaterium 39 38 37 36 Staphylococcus auricularis 43 42 41 40 Actinobacillus Urea 27 26 26 25 Bacillus succulence 32 32 31 30 Pseudomonas Eruzinosa 31 30 30 29 Pseudomonas aeruginosa-1 33 33 32 31 Staphylococcus aureus MRSA 31 30 30 29 Staphylococcus aureus MSSA 31 30 30 29 Unit mm (Clean Area Diameter)

Antifungal test First composition Second composition Third composition Fourth composition Neurospora Cytopilia 54 53 51 51 Tanidium Elegance 59 58 56 56 Raizofus Delimer 65 63 62 61 Penicillium Notatum 22 21 21 20 Aspergillus Oraijae 38 37 36 35 Altenaria Altenata 58 57 55 55 Muco Roissy 31 30 30 29 Tricorderma Squadron 43 42 41 40 Aspergillus Niger 22 21 21 20 Muco Musedo 19 19 18 18 Pichia Membra Napaenance 31 29 24 40 Zaigo Saccharomyces japonicus 27 26 25 32 Unit mm (Clean Area Diameter)

As can be seen from the results, it can be seen that the composition is much superior to the antimicrobial and antifungal power than when using the herbal extract alone (Experimental Example 1 to Experimental Example 2).

As described above, the composition of the present invention exhibits a broader and better antimicrobial, antifungal and bactericidal effect against various bacteria, fungi and yeast than when each essential oil is used alone, and 100% using essential oils harmless to the human body. Since it is composed of natural ingredients and does not have residual toxicity, it can be used as an environmentally friendly antibacterial and antifungal agent. In addition, the composition of the present invention can be used as an antibacterial and antifungal agent for both fragrance due to the unique natural flavor of each essential oil, it can also be applied to aromatherapy using the pharmacological action of each essential oil.

Claims (8)

i) 0.1 to 99.9 weight percent of oregano; And ii) Dill, Lavender, Lemon Balm, Rosemary, Mint, Savory, Sage, Sage, Scentedgeranium, Self Heal ( Self hill, Sweet Basil, Chervil, Thyme, Tansy, Cinnamon, Clove, Eucalyptus, Lemon, Peppermint ) And 0 to 99.9 wt% of one or more essential oils selected from Tea tree. The antimicrobial and antifungal composition according to claim 1, which comprises i) 0 to 80% by weight of oregano, ii) at least 0 to 70% by weight of at least one essential oil selected from lavender, lemon balm, thyme, eucalyptus, lemon and tea tree. Active composition i) 0.1 to 99.9 weight percent of Manuka; And ii) dill, lavender, lemon balm, rosemary, mint, savory, sage, cented geranium, self heel, sweet basil, chervil, thyme, tanji, cinnamon, clove, eucalyptus, lemon, peppermint and tea tree A composition having antimicrobial and antifungal action, characterized in that it comprises 0 to 99.9% by weight of one or more essential oils. The antimicrobial and antifungal composition according to claim 1, comprising 0 to 80% by weight of i) Manuka, and ii) 0 to 70% by weight of one or more essential oils selected from lavender, lemon balm, thyme, eucalyptus, lemon and tea tree. Active composition i) 0.1 to 99.9 weight percent of oregano, 0.1 to 99.9 weight percent of Manuka; And ii) dill, lavender, lemon balm, rosemary, mint, savory, sage, cented geranium, self heel, sweet basil, chervil, thyme, tanji, cinnamon, clove, eucalyptus, lemon, peppermint and tea tree A composition having antimicrobial and antifungal action, characterized in that it comprises 0 to 99.9% by weight of one or more essential oils. 6. The composition of claim 5, comprising i) 0-80% by weight of oregano, 0-80% by weight of Manuka, ii) 0-70% by weight of one or more essential oils selected from lavender, lemon balm, thyme, eucalyptus, lemon and tea tree. Composition with antibacterial and antifungal action, characterized in that The composition of any one of claims 1 to 6 used as a fragrance. The composition of any one of claims 1 to 6 used for aromatherapy