KR102283663B1 - Antimicrobial composition comprising extracts of herb - Google Patents

Abstract

The present invention relates to an antibacterial composition comprising an herbal extract as an active ingredient, which effectively removes periodontal disease-causing bacteria that cause bad breath and periodontal disease, thereby having excellent preventive effect and bad breath removal effect of periodontal disease and high antibacterial activity. Eggplant can provide an antibacterial composition.

Description

Antibacterial composition comprising herbal extract as an active ingredient {ANTIMICROBIAL COMPOSITION COMPRISING EXTRACTS OF HERB}

The present invention relates to an antibacterial composition comprising an herbal extract as an active ingredient. In more detail, an herbal extract selected from the group consisting of rosemary, lavender, sage, basil, mint, chamomile, oregano, lemon balm, carob, and mixtures thereof as an active ingredient has excellent effects of removing bad breath and preventing periodontal disease. It relates to an antibacterial composition having antibacterial activity.

Common diseases occurring in the oral cavity include tooth decay and bad breath from periodontal disease. Tooth decay is a disease in which bacteria and other microorganisms living in the mouth damage the enamel of the tooth surface by acid generated while decomposing sugar. In addition, periodontal disease is inflammation of the gums and periodontium due to plaque formed by bacteria that adhere to sugars in food and firmly attached to teeth like moss, and toxic substances that are metabolites of those bacteria, and calculus, which is a calcified material of the bacterial film on the tooth surface. This disease is divided into gingivitis, which is an inflammation of the gums, and periodontitis, which is an inflammation of the gums. The tooth decay and periodontal disease proceed without any symptoms, and they do not cause toothache, except for occasional bleeding or bad breath, so it is common to not know that periodontitis and tooth decay are progressing. Periodontal tissue and teeth are damaged to the extent that it is difficult to do so, and natural restoration is not possible. In addition, bad breath is the smell of exhaled breath coming out of the mouth, and generally refers to a smell that is unpleasant to the people around it. There are various factors for such bad breath, but in general, proteins, food waste, etc. in saliva are decomposed by bacteria and other microorganisms in the oral cavity, and the amino acids produced at this time are decomposed by amino enzymes, etc. to produce substances that cause bad odor.

Several species are known as pathogenic microorganisms that cause tooth decay, periodontal disease, and bad breath, but among them, Streptococcus

Mutans (Streptococcus mutans) and Porphyromonas gingivalis are known as representatives. The microorganisms secrete an enzyme called glucosyltransferase (GTase) from the outside or the surface of the cells, thereby using sucrose in food as a substrate to produce glucose and fructose, and at the same time glucose Insoluble glucan, a polymer of As other microorganisms adhere to the formed glucan on the tooth surface, a dental plaque is formed. In this plaque, lactic acid bacteria proliferate and produce lactic acid using fructose as a carbon source, and the lactic acid is collected and concentrated in the adherent glucan. It causes tooth decay by dissolving phosphorus enamel. In addition, pathogenic microorganisms in the oral cavity form plaque, which is a bacterial film, on the tooth surface, and the calcium ions (Ca2+) of the teeth are lost by accumulating high concentration of lactic acid generated from lactic acid bacteria proliferating in the plaque. As a result, tooth decay is induced along with the dissolution of the outer membrane enamel, and toxic substances induced from plaque seep into the crevice between the teeth and gums to cause an intracellular inflammatory reaction, leading to periodontal diseases such as gingivitis and periodontitis.

Currently, as a method of suppressing tooth decay, periodontal disease and bad breath, antibiotics such as spiramycin, vancomycin, and chlorhexidine are used as well as toothpaste and mouthwash containing organic or inorganic fluorine. There is a method to suppress pathogenic bacteria in the oral cavity. However, the method using antibiotics has a disadvantage in that microorganisms become resistant or can cause side effects such as diarrhea and vomiting, as well as inhibiting the growth of flora in the oral cavity due to the abuse of these antibiotics.

On the other hand, toothpaste is a quasi-drug used for brushing the surface of teeth, and it is the most common oral cleaning product in life for the purpose of sterilization, interdental cleaning and whitening. However, in a toothpaste composition generally used, 0.05 to 0.1 wt% of fluorine is used as an active ingredient and as an abrasive, a wetting agent, a foaming agent, and a sweetener. Rather, it has been found that there is a side effect that promotes inflammation of the gums.

Therefore, there is a need for a functional toothpaste composition for preventing and improving tooth decay, periodontal disease, and bad breath using a natural substance known to have fewer side effects than the compound fluorine as an active ingredient.

KR 10-2009-0130892 A

An object of the present invention is to provide an antibacterial composition comprising a herbal extract as an active ingredient.

Another object of the present invention is to use an herbal extract selected from the group consisting of rosemary, lavender, sage, basil, mint, chamomile, oregano, lemon balm, carob and mixtures thereof as an active ingredient, which is excellent in removing bad breath and preventing periodontal disease. At the same time, it is to provide an antibacterial composition having high antibacterial activity.

In order to achieve the above object, the antibacterial composition according to an embodiment of the present invention is an effective herbal extract selected from the group consisting of rosemary, lavender, sage, basil, mint, chamomile, oregano, lemon balm, carob and mixtures thereof. It is included as an ingredient, and it is excellent in the effect of removing bad breath and preventing periodontal disease.

The composition further comprises a natural oil, wherein the natural oil is selected from the group consisting of spike lavender oil (Lavandula latifolia oil), black pepper oil (Piper nigrum), Wheat Germ Oil, and mixtures thereof. include as

The composition is Streptococcus mutans (Streptococcus mutans), Actinobacillus actinomycetemcomitans (Actinobacillus actinomycetemcomitans), Porphyromonas gingivalis (Porphyromonas gingivalis), Tannerella forsythia (Tannerella forsythia) It has an antibacterial effect by effectively removing one or more bacteria selected from the group consisting of denticola (Treponema denticola) and Fusobacterium nucleatum.

The extract is extracted using an extraction solvent selected from the group consisting of water, C ₁ to C _{6 lower alcohols, and mixtures thereof.}

A toothpaste composition according to another embodiment of the present invention is prepared by using the antibacterial composition.

Oral spray according to another embodiment of the present invention is prepared using the antibacterial composition.

A gargle solution according to another embodiment of the present invention is prepared using the antibacterial composition.

Hereinafter, the present invention will be described in more detail.

As used herein, the term 'extract' has the meaning commonly used as a crude extract in the art as described above, but in a broad sense also includes a fraction obtained by additionally fractionating the extract. That is, the extract includes not only those obtained by using the above-described extraction solvent, but also those obtained by additionally applying a purification process thereto. For example, a fraction obtained by passing the extract through an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (prepared for separation according to size, charge, hydrophobicity or affinity), etc. The fraction obtained through the purification method is also included in the extract of the present invention.

The antibacterial composition according to an embodiment of the present invention contains an herbal extract selected from the group consisting of rosemary, lavender, sage, basil, mint, chamomile, oregano, lemon balm, carob and mixtures thereof as an active ingredient, and removes bad breath And it is excellent in the prevention effect of periodontal disease.

Herbal plants are herbaceous plants whose flowers, seeds, stems, leaves, and roots are useful for humans. Since ancient times, they have played an important role as medicinal herbs for pain relief and sedation, as well as for antiseptic and insecticidal properties. It is a natural material that is attracting more and more attention due to the toxicity of the material, concerns about environmental pollution, and its excellent efficacy.

Rosemary is a perennial evergreen deciduous tree in the family Lamiaceae that usually grows over 1 meter. The narrow, thin needle-shaped leaves have a leather-like quality, are shiny, have a characteristic strong aroma, and are dark green. It has a sterilizing and disinfecting effect, so it was used to preserve meat, and it was often used in hospitals or when infectious diseases were prevalent. Rosemary helps to improve memory, revitalizes the symptoms of lethargy, and relieves headaches and migraines. In addition, it has an anti-obesity effect, regenerates the skin, revitalizes the sagging tissue, suppresses the occurrence of edema and dandruff, and promotes hair growth. Rosemary is used for cooking, facial cleansing, and to increase blood pressure when you have low blood pressure.

Lavender is the most well-known variety of herbs. It is popular as an ornamental herb because the aromatic oil component makes the leaves and flower surfaces appear shiny, and their splendor shines even more during the flowering period. High-quality lavender is used for many purposes, and in particular, it is suitable for ornamental purposes as well as potpourri and various cosmetic products because it has a fragrance throughout the plant, such as flowers, leaves, and stems. Dried flowers are dried in the morning by cutting the purple buds before flowering and hanging them in the shade with good ventilation. For ornamental use, cut off after the flowers have faded, leaving about 2 stalks. The lotion made with the essential oil of lavender is effective for rough skin because it has a cleansing effect that relaxes and relieves the skin and regenerates it clean and moist. Also, the scent of lavender has a calming effect, so it was put in a pillow and used for sleep. When you drink lavender tea, it has a calming effect, relieves pain and headaches, and helps you sleep by changing your mood.

Sage has been used as a panacea since the Greek and Roman times. It is used in a variety of ways, such as ornamental, spice collection, medicine, cooking, dyeing, etc., and has tonic, soothing, digestive, and sterilizing effects. Sage has many uses, but should not be used in large quantities at once. Sage tea clears the mood, calms excitement, and is effective in preventing stomatitis, bleeding gums, and bad breath, but avoid drinking continuously because the effect is strong. In addition, sage promotes digestion, has a detoxifying effect, a placebo, and a bathing agent is used as a fatigue recovery effect, expectorant, gout, diuretic, anthelmintic, and antiseptic. It is very effective for menopausal disorders.

Basil is an annual plant that exudes a strong fragrance from the whole plant. The stem is dull square and resembles a perilla stem, and the lower stem is woody and green or purple. The basil is an herbal plant with a sweet and strong fragrance, and is widely used as a spice and tea, and has a great effect on migraine headache, antibacterial action, bad breath and promoting digestive function.

Mint is a native of Europe, and along with lemon balm, it can be said to be a representative herb of wheat source plants. It is a perennial plant of the Lamiaceae family and one of the strongest herbs to grow. It has a strong peppermint scent that gives a refreshing and refreshing feeling just by rubbing the leaves. For storage, cut off the stems of the stems in June and July, when the scent is strongest, and dry them in the shade. Mint herbs, characterized by a refreshing feeling, are especially used for making sauces for meat dishes, and with their refreshing taste and scent, they are used to flavor beanies, carbonated water drinks, cocktails, etc. Although there are some differences depending on the type of mint, it is used as a raw material for sterilization, digestion promotion, gastric action, oral deodorant, toothpaste, and placebo. It has medicinal effects on dermatitis and itching, and if left in the bathroom, the mint scent lasts for a long time instead of the bad smell, and it even has a strong sterilization effect. Green mint, also called spearmint, is often used in cooking along with peppermint. It has a sweeter flavor than peppermint and is very good for the skin.

Chamomile is one of the most well-known herbs as a medicinal herb among many herbs. It grows widely in Europe, India and North Africa and belongs to the Asteraceae family. It smells like sweet and sour apples, but in spring, tiny flowers bloom all at once, spreading a sweet fragrance. Dried flowers have excellent cosmetic effects and are used as a bathing agent. If you add a small amount to massage oil, it is good for recovery from fatigue after exercise. In Europe, when it comes to decoction, it reminds us of chamomile, but the genus name "Matricaria" is also effective in the uterus. Low inflammation, antiseptic, anthelmintic, good for relieving cramps. In addition, it has the effect of warming the body for those who have a cold. On the 2nd or 3rd day after flowering, the flowers have a good fragrance and taste. Incense relieves nervousness, anger, or severe mental tension. If you put 5 or 6 small white flowers in hot water, you can enjoy fresh tea with a sweet apple flavor. It is good to drink every day when you have stomach pain.

Oregano (Oregano) is a perennial plant of the leaf family, and its origin is Europe. It is used well in Italian and Mexican cuisine, and has a wild scent as it is nicknamed "wild" marjoram. It goes well with Latin dishes and tomatoes. It promotes digestion and has a tonic effect, and is used as a tea, bath, and dried flowers.

Lemonbalm is a cold-resistant perennial plant with side ailments and hairs, and a strong lemon scent. Drinking the lemon balm as a tea helps digestion and the extract of lemon balm has a sweating effect and is known to be good for colds.

Carob {Ceratonia siliqua (L.) Taub.} is an evergreen broad-leaved shrub up to 10 m tall and drought tolerant. The leaves contain resin. Small, dull greenish-brown flowers bloom in autumn, with long green pods running. These pods turn chocolate when ripe and contain small, shiny hard beans. Carob gum, which softens the skin, is popular as a skin pack and is also used to treat diarrhea.

In general, when a natural plant known for its detergency or antibacterial power is used as a composition for mouthwash as it is, it is common that the expected cleansing or antibacterial effect does not occur. In general, even in the case of extracts from natural products with known antibacterial or cleaning effects, most of them do not effectively remove plaque formed on teeth, and mutans (Streptococcus mutans), Gingivalis (Gingivalis), which cause periodontal disease in the oral cavity, etc. ) because it cannot effectively kill harmful bacteria such as In particular, the oral cavity is an environment in which food, beverages, saliva, etc. continuously move to a humid environment, so that contamination occurs continuously, and the cleaning agent is continuously washed and removed. In particular, this is because extracts of general natural products cannot remove harmful bacteria due to the environment where plaque cannot be removed.

On the other hand, alcohol or natural extracts with excellent cleaning effect can kill harmful bacteria in the oral cavity, but there is a problem in that it also kills beneficial bacteria in the oral cavity, so there is a problem in accelerating periodontal disease.

The composition further comprises a natural oil, wherein the natural oil is selected from the group consisting of spike lavender oil (Lavandula latifolia oil), black pepper oil (Piper nigrum), Wheat Germ Oil, and mixtures thereof. include as

Spike lavender oil (Lavandula latifolia oil) is an essential oil extracted from lavender spike flowers that refreshes the mind and is effective in relieving muscle pain and rheumatic pain. The Mediterranean, such as France and Spain, is the main production area. It is an essential oil extracted by steam distillation (a method of extracting oil from a plant with steam and cooling it with cooling water to condense it) from the flowers of Lavender Spike, a plant of the Lamiaceae family. A colorless to pale yellow transparent liquid, Lavender Spikes is a mixture of fresh floral and grassy notes of slightly sharp camphor, similar to a blend of lavender and rosemary. The main chemical composition consists of 1,8-cineole oxide, linalool, and Borneol alcohol, and camphor ketone. It helps to clear and refresh the mind, relieve stress and relieve headaches. It is effective in relieving cold symptoms such as cough and phlegm by its expectorant action, and in relieving pain such as sprains, muscle pain, arthritis, and rheumatism by its analgesic action. It is useful for wound healing and insect repellent.

Black pepper oil (Piper nigrum) is an essential oil extracted from the dried fruits of black pepper. It is an essential oil extracted by steam distillation by drying the unripe fruits of black pepper grown in South India and Indonesia. A colorless or light green liquid with woody and pungent aromas, it belongs to the base notes (long lasting fragrances). The main chemical components are monoterpenes such as alpha-pinene and β-pinene, and sesquiterpenes such as β-caryophyllene and α-humulene. It is effective in relieving digestive system disorders such as indigestion, gas filling, and constipation by warming the body. It is effective in relieving rheumatism, arthritis, muscle pain, bruises, and is also used on bruised areas.

Wheat Germ Oil is a vegetable oil extracted from wheat germ and is used as a carrier oil in aromatherapy. Rich in essential fatty acids and vitamin E, it has moisturizing and antioxidant effects on the skin. It is extracted from the germ of wheat, a plant growing worldwide, and it is difficult to extract by pure cold-pressing method. It is a vegetable oil extracted. It is viscous, has a characteristic strong odor, and is yellow to brown in color. Its main ingredients include linoleic acid and oleic acid, which are unsaturated fatty acids, and palmitic acid and stearic acid, which are saturated fatty acids. Rich in vitamin E, it acts as a natural antioxidant, has a moisturizing effect on the skin, is useful for cracked and dry skin, and has a cell regeneration effect, so it is effective in relieving scars, burns, and wrinkles. It lowers cholesterol and promotes blood circulation to help heart health. It is also sold as a health functional food because it is rich in nutrients.

When the natural oil is used, it is possible to effectively remove harmful bacteria such as Streptococcus mutans and Gingivalis, which cause periodontal disease in the oral cavity, as well as being effective in removing plaque, In the washing step, the plaque is removed together, so that even harmful bacteria remaining in the plaque can be more effectively removed.

In addition, as the spike lavender oil, black pepper oil, and ointment oil are additionally used in combination, the effect of inhibiting harmful bacteria causing periodontal disease is increased compared to the case of using only the herbal extract, removing bad breath, periodontal disease It is possible to provide a composition excellent in the prevention and improvement effect of

Preferably, the composition of the present invention may contain 20 to 40 parts by weight of spike lavender oil, 20 to 40 parts by weight of black pepper oil, and 20 to 40 parts by weight of the above-mentioned oil based on 100 parts by weight of the herbal extract. When used as a complex extract within the above range, while exhibiting the effect of removing bad breath and preventing periodontal disease, it enables the provision of an antibacterial composition having an excellent antibacterial effect.

The composition is Streptococcus mutans (Streptococcus mutans), Actinobacillus actinomycetemcomitans (Actinobacillus actinomycetemcomitans), Porphyromonas gingivalis (Porphyromonas gingivalis), Tannerella forsythia (Tannerella forsythia) It has an antibacterial effect by effectively removing one or more bacteria selected from the group consisting of denticola (Treponema denticola) and Fusobacterium nucleatum.

Periodontal disease is a disease in which the gums, periodontal ligaments, and bone tissue supporting the teeth become inflamed, and is often referred to as gingivitis. It is a relatively light and quick form of periodontal disease, and the form limited only to the gums, that is, the soft tissue is called gingivitis, and when the inflammation has progressed to the gums and around the gums, it is called periodontitis. There is a V-shaped gap between the gingiva (gum) and teeth. Oral pathogens attack the lower part of the gum line of the sulcus and release lipopolysaccharide (LPS), which is an inflammatory stimulant. Inflammation occurs, such as swelling and bleeding of the gums, which damages the periodontal ligaments and adjacent tissues. As periodontitis progresses, periodontal ligaments and even alveolar bone are damaged and eventually teeth are lost. Nutritional deficiencies such as protein and vitamins, hormonal disorders such as pregnancy or diabetes, smoking, and Acquired Immune Deficiency Syndrome (AIDS) can aggravate the disease. In addition, other causes of periodontal disease include plaque and tartar. Periodontal disease of the present invention is included regardless of the type of bacteria causing periodontal disease, specifically, Streptococcus mutans, Actinobacillus actinomycetemcomitans (Actinobacillus actinomycetemcomitans), Porphyromonas At least one uniform selected from the group consisting of Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola and Fusobacterium nucleatum, and , more specifically P. gingivalis.

Halitosis is an odor originating from the oral cavity and adjacent organs. The main component of bad breath is volatile sulfur compounds, and 90% of the total amount of volatile sulfur compounds is hydrogen sulfide made from cysteine and methyl mercaptan and dimethyl sulfide made from methionine. These components are mainly produced by protein enzymes secreted by anaerobic bacteria, and the back of the tongue is the most important habitat. This area is not well cleaned by saliva, and there are many small depressions, making it a place for bacteria to live continuously. Although the production of volatile sulfur compounds by anaerobic bacteria is the most important cause of bad breath, it is also caused by oral diseases such as dental caries, periodontitis, and dry mouth. Many types of anaerobic bacteria are involved in the occurrence of bad breath, and a non-limiting example of the bacteria causing bad breath may include Porphyromonas gingivalis, which secretes many types and large amounts of enzymes.

Therefore, the antibacterial composition of the present invention can exhibit the effect of reducing bad breath and preventing periodontal disease by effectively removing Porphyromonas gingivalis, which is a harmful bacterium that causes bad breath and periodontal disease.

The extract is extracted using an extraction solvent selected from the group consisting of water, C ₁ to C _{6 lower alcohols, and mixtures thereof.}

Specifically, pulverizing a natural product to prepare an herbal extract; leaching the pulverized product using an organic solvent; drying the sample after leaching; re-leaching the dried sample using an organic solvent; drying the sample after leaching; leaching with water; And including the step of leaching, it is possible to obtain an herbal extract.

The natural extract extracted using the organic solvent may further include the step of performing fractionation using an organic solvent.

The extraction solvent may be used in an amount of 2 to 50 times, more specifically, 2 to 20 times, based on the weight of the sample. For extraction, the sample may be left for 1 to 72 hours for leaching in the extraction solvent, and more specifically, for 24 to 48 hours.

The extract may be prepared in a powder state by additional processes such as vacuum distillation and freeze drying or spray drying, and is obtained by an extraction method selected from the group consisting of solvent extraction method, ultrasonic extraction method, reflux extraction method, leaching method, fermentation method and foaming method. include that

In ultrasonic extraction, the reaction is carried out at 30 to 50° C. for 0.5 to 2.5 hours, and the extraction solvent is water or 50 to 100% alcohol having 1 to 6 carbon atoms. Specifically, extraction is performed at 40 to 50° C. for 1 to 2.5 hours, and the extraction solvent is water or 70 to 80% alcohol having 1 to 6 carbon atoms.

The reflux extraction method is based on 100 mL of water, an alcohol having 1 to 6 carbon atoms, 10 to 30 g of a pulverized product of a natural product, a reflux time of 1 to 3 hours, and 50 to 100% alcohol or water having 1 to 6 carbon atoms. More specifically, based on 100 ml of an alcohol having 1 to 6 carbon atoms or 100 ml of water, 10 to 20 g of a pulverized product of a natural product, a reflux time of 1 to 2 hours, and 70 to 90% of an alcohol or water having 1 to 4 carbon atoms.

The leaching method is carried out at 15 to 30° C. for 24 to 72 hours, and water or 50 to 100% alcohol having 1 to 6 carbon atoms is used as an extraction solvent. More specifically, the process is carried out at 20 to 25° C. for 30 to 54 hours, and the extraction solvent is water or 70 to 80% alcohol having 1 to 6 carbon atoms.

After extraction, the extract may be fractionated by sequentially applying a fresh fractionation solvent. The fractionation solvent used for fractionation is any one or more selected from the group consisting of water, hexane, butanol, ethyl acetic acid, ethyl acetate, methylene chloride and mixtures thereof, preferably ethyl acetate or methylene chloride.

A toothpaste composition according to another embodiment of the present invention is prepared by using the antibacterial composition.

The toothpaste composition includes surfactants, abrasives, stabilizers, binders, foaming agents, fragrances, pigments, antibacterial agents, antioxidants, preservatives, wetting agents, and thickeners commonly used in the art in addition to the herbal extract and natural oil of the present invention. , inorganic salts, whitening agents, emulsifiers, buffers, sweeteners, solubilizers, tissue strengthening agents, and may further include additives such as mouthwashes. The component is sufficient as long as it is a component commonly used in the art, and the amount of the component can be adjusted within a range that does not impair the desired effect of the present invention by selection of those skilled in the art. In more detail in the additive, abrasives such as dibasic calcium phosphate, calcium carbonate, calcium pyrophosphate, calcium sulfate, sodium metaphosphate, silica, aluminum silicate, aluminum hydroxide (usually 20 to 60% used), carboxymethyl cellulose, Thickeners such as hydroxyethyl cellulose, alginate, carrageenan, gum arabic, polyvinyl alcohol, etc. (1.5-5%), polyethylene glycols, sorbitol, glycerin, propylene glycol, etc., sodium lauryl sulfate, sodium lauryl sarco Surfactants such as cinate, fragrances including menthol and mint, sweeteners such as saccharin and stevioside, and preservatives, sodium fluoride, sodium monofluorophosphate, tin fluoride, salt, allantoin, vitamins, quaternary ammonium compounds Various active ingredients commonly used in toothpaste, etc. may be mixed with water and used as a toothpaste composition.

Oral spray according to another embodiment of the present invention is prepared using the antibacterial composition.

A gargle solution according to another embodiment of the present invention is prepared using the antibacterial composition.

The gargling liquid is defined as including all of the composition capable of gargling the oral cavity and other preparations for cleaning and antibacterial activity in the oral cavity. On the other hand, the gargle liquid to achieve the object of the present invention should be a composition based on herbal extracts and oils, and it is preferable not to mix a surfactant or alcohol. The specific formulation is defined as including both the composition and mixing range that can be selected and mixed by a person skilled in the art.

According to the antibacterial composition comprising the herbal extract of the present invention as an active ingredient, it effectively removes periodontal disease-causing bacteria that cause molar teeth and periodontal disease, and has excellent preventive effect and bad breath removal effect of periodontal disease and high antibacterial activity. An antibacterial composition may be provided.

Hereinafter, embodiments of the present invention will be described in detail so that those of ordinary skill in the art can easily carry out the present invention. However, the present invention may be embodied in many different forms and is not limited to the embodiments described herein.

[Preparation Example 1: Preparation of herbal extract]

1. Preparation of Rosemary Extract

The rosemary was thoroughly washed under running water and then completely air-dried. The dried rosemary was pulverized with a blender and then prepared into powder. 50% ethanol as an extraction solvent was added to the powder sample at a ratio of 1;10 (w; v), and then completely immersed, and extracted three times for 3 hours while refluxing at 80°C. The extract is Whatman No. 2 filtered with filter paper. The filtrate was concentrated under reduced pressure at 60° C. to prepare a rosemary extract (RH).

2. Preparation of natural extracts

Using the same method as the production method of the rosemary extract (RH), lavender extract (LE), sage extract (SE), basil extract (BH), mint extract (ME), chamomile extract (CH), oregano extract (OH) ), lemon balm extract (IH) and carob extract (TH) were prepared.

3. Preparation of Complex Herbal Extracts

The rosemary extract (RH), lavender extract (LE), sage extract (SE), basil extract (BH), mint extract (ME), chamomile extract (CH), oregano extract (OH), lemon balm extract (IH) and 900 g of herbal extract (MH) was prepared by mixing 100 g each of carob extract (TH).

[Preparation Example 2: Preparation of antibacterial composition]

An antibacterial composition was prepared by mixing the herbal extract (MH), spiked vender oil (LO), black pepper oil (BO), and top point oil (WO) prepared in Preparation Example 1 as shown in Table 1 below.

A1 A2 A3 A4 A5 A6 MH 100 100 100 100 100 100 LO - 10 20 30 40 50 BO - 10 20 30 40 50 WO - 10 20 30 40 50

(Unit: parts by weight)

[Experimental Example 1: Antibacterial effect]

1. Antibacterial effect on bacteria causing bad breath and periodontal disease

(1) mutans (Streptococcus mutans) antibacterial activity against bacteria

Streptococcus mutans, known as a representative harmful bacteria of periodontal disease, was inoculated into a solid medium and cultured for 3 to 4 days. In the control group, only purified water was treated, and the A1 (ME) to A6 preparations were treated and then cultured 24 hours later.

Growth inhibitory ability (%) was investigated by measuring the absorbance at 650 nm before and after the treatment of the purified water and Preparation Example.

For objective comparison, the result (control) using purified water was set to 100, and the value of each Example was divided by the value of the growth inhibitory ability of the control to allow comparative evaluation. The results (antibacterial activity against mutans bacteria: Mu) are shown in Table 2 below. In the values shown in Table 2, the lower the number, the better the antibacterial power against the bacteria Streptococcus mutans.

(2) Antibacterial activity against Gingivalis bacteria

In order to evaluate the antibacterial ability against Gingivalis, a well-known causative agent of periodontal disease and bad breath, gingivalis was inoculated into a solid medium and cultured for 3 to 4 days. In the control group, only purified water was treated, and the A1 (ME) to A6 preparations were treated and then cultured 24 hours later.

Growth inhibitory ability was investigated by measuring the absorbance at 650 nm before and after the treatment of the purified water and Preparation Example.

For objective comparison, the result (control) using purified water was set to 100, and the value of each Example was divided by the value of the growth inhibitory ability of the control to allow comparative evaluation. The results (antibacterial activity against Gingivalis bacteria: Gv) are shown in Table 2 below. In the values shown in Table 2, the lower the number, the better the antibacterial power against Gingivalis bacteria.

(3) Lactobacillus reuteri (Lactobacillus reuteri) bacteria preservation test

Lactobacillus reuteri, known as one of the representative beneficial bacteria that prevent tooth decay by inhibiting mutans and other periodontal disease prevention, was cultured in a medium, inoculated in a solid medium, and cultured for 3 to 4 days. . In the control group, only purified water was treated, and after treatment with Preparation Examples A1 (ME) to A6, culture was performed after 24 hours.

Growth inhibitory ability was investigated by measuring the absorbance at 650 nm before and after the treatment of the purified water and Preparation Example.

For objective comparison, the result (control) using purified water was set to 100, and the value of each Example was divided by the value of the growth inhibitory ability of the control to allow comparative evaluation. The results (preservation test for Lactobacillus reuteri: Rt) are shown in Table 2 below. The values shown in Table 2 indicate that the higher the number, the better the preservation ability for Lactobacillus reuteri bacteria.

Mu Gv Rt Control 100 100 100 A1 9.79 7.41 24.71 A2 4.89 5.11 24.41 A3 3.96 4.04 44.17 A4 4.13 3.94 43.35 A5 4.97 2.14 41.25 A6 7.81 6.14 24.46

(Unit: Index)

As shown in Table 2, in the case of the antibacterial compositions (A2 to A6) containing the herbal extracts (A1, MH) and natural oils, the cleaning effect of rapidly reducing the number of bacteria was confirmed.

In particular, in the case of the antimicrobial composition mixed in the range of A3 to A5 containing natural oil, it was confirmed that the antibacterial activity of harmful bacteria is doubled and increased due to the synergistic effect by the interaction. In addition, it can be seen that the effect of preventing the beneficial bacteria from disappearing is the highest.

Therefore, when according to the above range, it is possible to provide an antibacterial composition capable of effectively removing harmful bacteria with a smaller content and preventing beneficial bacteria from being killed in the oral cavity, and removing bad breath by effectively removing harmful bacteria that cause periodontal disease , it can exhibit the effect of preventing and improving periodontal disease.

[Experimental Example 2: Plaque Removal Effect]

Purified water (Control) and Preparation Examples A1 (ME) to A6 were provided to 20 testers as gargles, and the plaque removal effect before and after gargling was tested.

The degree of plaque removal was evaluated and the results are shown in Table 3 below. The degree of plaque removal was evaluated as related through plaque staining, and the result was evaluated as an index of 1 to 10 based on 5 purified water. For each index, the higher the number, the better.

Control A1 A2 A3 A4 A5 A6 plaque 5 6 7 8 9 8 6

(Unit: Index)

As shown in Table 3, in the case of herbal extracts (A1, MH), it was found that the plaque removal effect was higher than that of purified water, and in the case of the antibacterial composition (A2 to A6) containing natural oil, the plaque removal effect was It was found that there was a noticeable increase.

[Experimental Example 3: Taste Test]

In the plaque removal experiment, the test subjects were asked to describe the taste by evaluating the refreshing feeling and flavor for each preparation example of A1 to A6. The evaluation was performed on a scale of 1 to 10 according to the 10-point method. In the index, the higher the number, the better the refreshing feeling and flavor.

The results are shown in Table 4 below.

A1 A2 A3 A4 A5 A6 refreshment 5 6 7 8 7 6 incense 3 4 6 8 7 5

(Unit: Index)

As shown in Table 4, the herbal extracts (A1, MH) had excellent taste for refreshing feeling, but had a relatively strong fragrance due to the use of the complex herbal extract, so it was confirmed that the preference for fragrance was low.

However, in the case of the antibacterial composition (A2 to A6) containing natural oil, the strong scent of the complex herbal extract was neutralized, and the preference for the scent increased, and in particular, according to A3 to A5, the preference for the refreshing feeling also increased. As a result, it was evaluated that the overall acceptance rate was definitely increased.

Although the preferred embodiment of the present invention has been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements by those skilled in the art using the basic concept of the present invention as defined in the following claims are also provided. is within the scope of the

Claims (7)

Contains herbal extracts including rosemary, lavender, sage, basil, mint, chamomile, oregano, lemon balm and carob as active ingredients,
Contains more natural oils,
The natural oil is Spike Bender oil (Lavandula latifolia oil), black pepper oil (Piper nigrum) and Wheat Germ Oil,
With respect to 100 parts by weight of the herb extract, 20 to 40 parts by weight of spiker bender oil, 20 to 40 parts by weight of black pepper oil, and 20 to 40 parts by weight of the above oil
Removal of bad breath and prevention of periodontal disease
antibacterial composition. delete The method of claim 1,
The composition has an antibacterial effect by removing Streptococcus mutans and Porphyromonas gingivalis bacteria.
antibacterial composition. The method of claim 1,
The extract is extracted using an extraction solvent selected from the group consisting of water, C ₁ to C _{6 lower alcohols and mixtures thereof.}
antibacterial composition. A toothpaste composition comprising the antimicrobial composition according to claim 1 . An oral spray comprising the antimicrobial composition of claim 1 . A gargle containing the antibacterial composition according to claim 1 .