KR20030009578A - Oral composition for inhibiting the halitosis - Google Patents

Abstract

PURPOSE: An oral composition for inhibiting halitosis including a specified amount of a green tea extract, cetylpyridium chloride and a pine needle extract is provided which has an excellent halitosis removing effect over a long term period. CONSTITUTION: The oral hygiene composition for inhibiting halitosis contains 0.005 to 2.0% by weight of a green tea extract, 0.01 to 5.0% by weight of cetylpyridium chloride and 0.005 to 2.0% by weight of a pine needle extract. For an example, 1kg green tea leave is soaked in 10kg ethanol for 7 days while agitating occasionally, filtered and concentrated under reduced pressure to give 400g concentrate. Also 50g pine needles with a size of 1.0 to 2.0cm are soaked in 500ml mixture of water and ethanol of 1:1 and extracted for 7 days to give a 50mg concentrate. Thereafter, these two extracts are mixed with cetylpyridium chloride and other additives.

Description

Oral composition for inhibiting bad breath {Oral composition for inhibiting the halitosis}

The present invention relates to an oral composition having an excellent bad breath inhibitory effect, and more particularly, to a composition for oral cavity having an excellent bad breath inhibitory effect by containing green tea extract, cetylpyridinium chloride and pine needle extract in a specific content.

Bad breath occurs when proteins, food residues, etc., present in saliva are decomposed by microorganisms in the oral cavity, and the amino acids produced by decalcification enzymes or amino enzymes are used to produce substances causing odor. The main components that cause bad breath are volatile sulfides, and these volatile compounds include hydrogen sulfide, methyl mercaptan, dimethyl mercaptan, and the like. In addition to these volatile sulfides, volatile amine compounds such as triethylamine, aldehydes, fatty acids, ammonia and the like.

Therefore, in order to suppress bad breath occurring in the oral cavity, it is necessary to remove microorganisms in the oral cavity, which is the primary cause of bad breath, or to remove a substance causing bad breath. In the related art, oral compositions for inhibiting bad breath containing flavonol components of green tea As can be seen, the bad breath has been mainly suppressed by removing the substances causing the bad breath.

However, the method of removing the substance causing bad breath can remove the bad breath in the short term, but there is a disadvantage that the long-term effect does not last.

Accordingly, the present inventors have studied to develop a composition for oral cavity that can not only suppress bad breath in the short term but also maintain the bad breath suppression effect for a long time, and can remove the substances causing bad breath and the causative agents of bad breath in the oral composition. It has been found that this object can be achieved by simultaneously containing the substance present.

On the other hand, conventionally used to sterilize such harmful bacteria in the oral cavity include cetylpyridinium chloride, chlorohexidine hydrochloride, etc. Chlorohexidine hydrochloride has the disadvantage of causing tooth discoloration, cetylpyridinium chloride is a cation As a compound having a strong reactivity with an anionic substance, there is a problem that the effect is sometimes halved depending on the oral environment in actual use.

Therefore, the present inventors study on a method for solving the problems of the above-described antimicrobial substances, while containing all the substances that can remove bad breath-causing substances and bad breath-causing bacteria in order to provide a composition for oral cavity excellent in inhibiting bad breath. As a result, green tea extract with excellent deodorant effect and cetylpyridinium chloride with excellent antibacterial effect are mixed in a specific content, and to supplement the effect of cetylpyridinium chloride, it is safe for human body and has good compatibility with other ingredients. It was found that the above object can be achieved by further mixing pine needle extract having excellent antibacterial effect in a specific content and completed the present invention.

In order to achieve the above object, the composition for oral cavity of the present invention is characterized in that it contains 0.005 ~ 2.0% by weight green tea extract, 0.01 ~ 5.0% by weight cetylpyridinium chloride and 0.005 ~ 2.0% by weight pine needle extract.

Hereinafter, the present invention will be described in more detail.

The composition for oral cavity of the present invention is a deodorant effect by the green tea extract, with ceftylpyridinium chloride as an antimicrobial agent with the effect of eliminating bad breath immediately after use, while removing the causative agents causing bad breath, while at the same time containing cetylpyridinium chloride oral environment This solves the problem that the effect is lowered.

First, looking at each of the components contained in the composition for oral cavity of the present invention are as follows.

(1) green tea extract

Green tea extract is a separation and purification of the active ingredient in tea leaves and is used in a variety of daily necessities such as toothpaste, deodorant, deodorant, cosmetics. In particular, it has been demonstrated through various experiments that the flavonol component of green tea exhibits a strong deodorant effect on oral odor components.

The present invention also utilizes this deodorant effect of green tea extract.

The method for preparing green tea extract may be prepared according to a conventional method of extracting an active ingredient from a plant. For example, a finely cut tea leaf is immersed in water or a polar organic solvent alone or a mixture thereof to obtain an extract. It can manufacture by the method of filtering and concentrating a filtrate under reduced pressure.

The composition for oral cavity of this invention contains this green tea extract in the quantity of 0.005 to 2.0 weight% with respect to the composition total weight. Generally, increasing the content of green tea extract increases the bad breath removal effect of the composition, but the stability of the composition decreases with time, and the value of the product decreases, such as browning phenomenon when stored at room temperature at a concentration exceeding 2.0% by weight. This is because there is a problem, and if less than 0.005% by weight, bad breath removal effect cannot be obtained.

(2) cetylpyridinium chloride

Cetylpyridinium Chloride (C ₂₁ H ₃₈ ClN · H ₂ O: 358.01) is a cationic ammonium salt antibacterial agent that sterilizes harmful bacteria in the oral cavity. It has excellent effect on gum disease and is widely used in oral products. In addition, it is said to be safe when used at a suitable low concentration, and is mainly used safely in oral hygiene products such as mouse wash.

Cetylpyridinium chloride exhibits bactericidal and growth inhibitory activity against anaerobic and aerobic bacteria causing various diseases in the oral cavity, and is known to be active against fungi including Candida albicans . The mechanisms identified are adsorption to cell walls, disrupting the metabolism of bacteria, inhibiting their growth and eventually destroying bacteria. In vivo experiments have revealed the effects on S. mutans and S. sanguis, and oral products containing cetylpyridinium chloride can reduce the accumulation of plaque-associated plaques and have effects on bacteria associated with periodontal disease. It has the effect of alleviating the degree of (Oral Hygiene Products and Practice, p333-338).

The composition of the present invention contains cetylpyridinium chloride having such an effect in an amount of 0.01 to 5.0% by weight based on the total weight of the composition. This is because cetylpyridinium chloride has a bitter taste peculiar to the raw material itself, so if the content exceeds 5.0% by weight, the bitter taste of the composition is too strong to be difficult to use, and less than 0.01% by weight of the antibacterial effect.

(3) pine needle extract

Pine is a genus of about 80-90 species in the world, and the terpenes collected from them are mainly used in industry. The main components of pine needles are Terpennetine oil, Cineole, Salinigrin, Coniferin, P-Cymen, Densipimaric acid, Retene, chlorophyll, protein, crude fat, phosphorus, iron, enzymes, minerals, fat-soluble vitamin A and vitamin C, etc., and these components vary slightly depending on the species of pine and the season harvested.

Conventional pine needles have been used for folk remedies known empirically and various effects. Among those related to oral disease, bad breath was used to chew a few pine needles in water, and for stomatitis, pineal fluid was applied to cotton wool and applied to affected areas. In addition, the toothache is brushed with boiled water, the gums are swollen, and the salt is added to the boiled pine needles. Salt is put in the mouth for a while, and the thrush is given a handful of pine needles and the juice is bitten in the mouth. Brushing teeth with water is effective (pine needle health method, pp. 56-147).

Since the composition of the present invention contains a pine needle extract that has been used in the conventional treatment of oral diseases, cetylpyridinium chloride can effectively inhibit bad breath by preventing the antibacterial effect of bad breath-causing bacteria caused by the oral environment. .

The preparation method of pine needle extract can be prepared according to a conventional method of extracting the active ingredient from the plant, for example, finely cut pine needles are immersed in water or a polar organic solvent alone or a mixture thereof to obtain the extract and then filtered. And the filtrate is concentrated under reduced pressure.

The composition of the present invention contains the pine needle extract in an amount of 0.005 to 2.0% by weight based on the total weight of the composition with respect to the total weight of the composition. This is because when it is formulated at less than 0.005% by weight, there is no antimicrobial effect against bad breath-causing bacteria, and when it exceeds 2.0% by weight, it is difficult to use because of its strong taste and peculiar smell.

The formulation of the composition for oral cavity of the present invention is not particularly limited, but may be prepared in various formulations of oral hygiene enhancers such as toothpaste and mouthwashes, and in the present invention, experiments with mouthwashes.

In addition to the above components, the mouthwash composition of the present invention may further contain a humectant, a sweetener, a pH adjuster, a preservative, a colorant, an astringent, a caries preventive agent and a medicinal ingredient, a foaming agent and a solubilizer, a perfume, a solvent, and the like.

Wetting agents include glycerin, sorbitol solution, polyethylene glycol, and propylene glycol.Sweeping agents include saccharin sodium, aspartame, stevioside, and licoriceic acid. Sodium phosphate, citric acid, sodium citrate, tartaric acid and the like, and preservatives include methyl paraoxybenzoate, propyl paraoxybenzoate, sodium benzoate.

Zinc chloride is used as astringent, and caries preventive agents include sodium fluoride, sodium monofluorophosphate, tin fluoride, and organic fluorine compounds. Etc. can be used individually or in mixture of 2 or more types.

As the foaming agent or solubilizer, nonionic surfactants such as sucrose fatty acid ester, polyoxyethylene hardened castor oil, sorbitan fatty acid ester, and polyoxyethylene polyoxypropylene copolymer are used alone or in combination of two or more thereof.

As the fragrance, peppermint oil, spearmint oil, menthol, carbon, and anetol may be used alone or in a mixture. As a solvent, ethanol, purified water may be used, and other food coloring may be used as a colorant.

Hereinafter, although an Example and a test example are given and this invention is demonstrated in detail, this invention is not limited only to these examples.

Reference Example 1 Preparation of Green Tea Extract

10 kg of 70% ethanol was added to 1 kg of green tea leaves, and the mixture was immersed at room temperature for 7 days with occasional stirring. The resulting filtrate was concentrated under reduced pressure to obtain 400 g of concentrate.

Reference Example 2 Preparation of Pine Needle Extract

50 g of collected pine needles were cut into 1.0-2.0 cm in size, and a 1: 1 mixed solution of water and ethanol was added at a rate of 500 ml. The mixture was immersed and extracted for 7 days at room temperature, and the filtrate was concentrated under reduced pressure. 50 mg of concentrate was obtained.

<Example 1 and Comparative Examples 1-4>

ingredient Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Example 1 weight% Sodium fluoride 0.30 0.30 0.30 0.30 0.30 Cetylpyridinium chloride - 0.05 0.05 - 0.05 Pine needle extract (Reference Example 2) 0.10 - 0.10 - 0.10 Green Tea Extract (Reference Example 1) - - - - 0.10 Concentrated glycerin 3.00 3.00 3.00 3.00 3.00 Sorbitol solution 10.00 10.00 10.00 10.00 10.00 Polyoxyethylene Cured Castor Oil 1.00 1.00 1.00 1.00 1.00 Zaccarin Sodium 0.10 0.10 0.10 0.10 0.10 Sodium benzoate 0.10 0.10 0.10 0.10 0.10 Citric acid 0.03 0.03 0.03 0.03 0.03 Spices 0.20 0.20 0.20 0.20 0.20 Elmenthol 0.20 0.20 0.20 0.20 0.20 Purified water to 100 to 100 to 100 to 100 to 100

Test Example 1 Comparative Test

2 ml of test solution diluted from Example 1 and Comparative Examples 1-4 from 1:20 to 1: 5120 and 18 ml of blood agar medium (blood agar base + final blood 5% sheep) were 9 cm petri dishes. Poured into. The test bacteria were then inoculated and incubated at 37 ° C. for 96 hours using an anaerobic chamber. The maximum dilution rate at which the test bacteria did not grow was measured by MID, and the results are shown in Table 1 (using the Bactron I. anaerobic chamber).

The test bacteria were Fusocacterium nucleatum ATCC 25586 and Porphyromonas gingivalis ATCC 33277.

Test bacteria Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Example 1 Husobacterium Nucleatum (Fusobacterium nucleatum) 320 640 2560 > 20 2560 Polfimonas Gingalis (Porphyromonas gingivalis) 160 320 1280 > 20 2560

Test Example 2 Measurement of the Number of Bacteria in Saliva

After dispensing 5 ml of sterilized 0.1% peptone solution (add 1 g of peptone to 1000 ml of 0.9% NaCl) in a 15 ml Falcon tube, 40 were prepared, and 40 subjects received water for 15 seconds. The peptone solution was diluted 10-fold with sterile 0.067 M phosphate buffer (pH 7.2). Take 100 μl of this diluted solution and dispense to Mitis Salivarius Agar, spread it on a plate with sterile bending glass, and then incubate for 12 hours in a 37 ° C incubator and incubate for 48 hours at 37 ° C in an anaerobic chamber. The colony of Streptococcus mutans in each medium was measured and recorded using a colony counter. The colonies of Streptococcus mutans were identified by microscopic examination, and the colonies of Streptococcus mutans were selected from the subjects by incubating the standard strain Streptococcus mutans NTCC 10449 in MSA at the same time and conditions. It was measured by. Diluted peptone water poloxial solution was dispensed to two plates of Mitis Salivarius Agar per test subject, and colony measurements were performed twice and two times, and the average value of the two measurements was determined by streptococcus mutans. ) Number .

Elapsed time Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Example 1 Right after the experiment 104 103 102 105 104 after 2 weeks 66 45 24 95 22

As can be seen from the results of Table 2, the composition of Comparative Example 4 containing no pine needle extract and cetylpyridinium chloride did not reduce the number of Streptococcus mutans almost. However, in the case of Comparative Example 2 containing cetylpyridinium chloride, unlike in Test Example 1, it showed a higher reception compared to Comparative Example 1 containing a pine needle extract. This suggests that cetylpyridinium chloride is affected by the oral environment in practical use. On the other hand, in the case of Comparative Example 3 in which cetylpyridinium chloride and pine needle extract is used together, excellent results were obtained. From this, it can be seen that pine needle extract supplements the antibacterial effect of cetylpyridinium chloride according to the oral environment.

Test Example 3 Deodorization Effect Measurement

Thirty subjects were allowed to chew 0.5 mg of garlic to cause bad breath in the oral cavity of the subjects. The rate of bad breath before and after was calculated.

The garlic chewing time was 2 minutes, and the amounts of methylmercaptan and allylmercaptan were measured and compared at 5, 10, 15, and 30 minutes after brushing, respectively.

The exhalation from the oral cavity was inserted into the oral cavity with the tip of the gas suction injection machine, and 10 ml of exhalation was collected. In order to analyze only methylmercaptan and allylmercaptan in expiratory air, a 2.0 ml sampling loop was attached to gas chromatography (HP5890). The detector was a FPD (Flame Photometric Detetector), and the colum was a Teflon tube filled with Chromosil 3-00 (Supelco) 6 ft long and 1/8 inch outside diameter. The column temperature was 70 ° C. and the carrier gas was helium at 20 mL / min.

Methylmercaptan amount reduction rate (%) Elapsed time after garlic Comparative Example 3 Example 1 5 minutes 58.4 85.4 10 minutes 65.2 88.0 15 minutes 77.5 91.5 30 minutes 80.1 95.6

Allyl mercaptan amount reduction rate (%) Elapsed time after garlic Comparative Example 3 Example 1 5 minutes 53.9 86.5 10 minutes 70.0 89.9 15 minutes 73.9 93.5 30 minutes 76.8 96.3

As can be seen from Table 3 and Table 4, the reduction rate of the amount of methyl mercaptan and allyl mercaptan after garlic operation compared to Comparative Example 3 in which Example 1 containing green tea extract in addition to pine needle extract and cetylpyridinium chloride did not contain green tea extract This was higher. Therefore, it was confirmed that the deodorizing effect is increased by the excellent deodorizing effect of green tea extract.

Test Example 4 Measurement of Bad Breath Removal Effect

After 80 hours of treatment, all oral environment management was stopped for 48 hours. After measuring bad breath with a harimeta, the test was divided into 8 groups so that the average value was similar. Each group was allowed to brush with Test Example 1 and Comparative Example 1-4. At 30 minutes after brushing, the degree of bad breath was measured again. Each test fern was then used twice a day for 28 days. Summoned at 7 days, 14 days, 21 days and 28 days, the degree of bad breath was measured again 30 minutes after brushing the teeth, and the results are shown in Table 5.

Elapsed time Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Example 1 Right after the experiment 220 225 218 221 170 7 days 204 210 171 224 112 14 days 195 197 142 220 83 21st 183 188 112 218 71 28 days 172 176 105 219 52

Table 5 shows the bad breath reduction effect according to the use period of the mouthwash composition, the bad breath score of the composition of Example 1 was mixed with green tea extract, cetylpyridinium chloride and pine needle extract was significantly reduced.

As described above, the composition for oral cavity according to the present invention can provide an excellent bad breath removing effect over a long period of time.

Claims (1)

Oral composition for inhibiting bad breath, characterized in that it contains green tea extract 0.005 ~ 2.0% by weight, cetylpyridinium chloride 0.01 ~ 5.0% by weight and pine needle extract 0.005 ~ 2.0% by weight.