KR102313830B1 - Composition for inhibiting halitosis containing seaweed extracts - Google Patents

Abstract

The present invention relates to a composition for alleviating bad breath comprising a seaweed extract as an active ingredient, and using the seaweed extract, it is effective to use a seaweed extract that exhibits antibacterial activity against oral microorganisms (pathogens) and a reduction in bad breath and has excellent human safety. It is possible to provide a composition for alleviating bad breath comprising as a component.

Description

Composition for alleviating bad breath comprising seaweed extract as an active ingredient {COMPOSITION FOR INHIBITING HALITOSIS CONTAINING SEAWEED EXTRACTS}

The present invention relates to a composition for alleviating bad breath comprising a seaweed extract as an active ingredient. More specifically, the present invention relates to a composition for alleviating bad breath, which uses a plurality of seaweed extracts to exhibit antibacterial activity against oral microorganisms (pathogens) and an effect of reducing bad breath, and at the same time have excellent human stability.

As the concept of dental health is changed to the concept of social life, interest in oral health is increasing, and as well as prevention and treatment of oral diseases, and rehabilitation of loss of function, there is also a study on bad breath, which is an important interpersonal disorder in social life. Interest is growing.

The causes of such bad breath are largely classified into oral causes, extraoral causes, and psychological causes. , increase in the amount and activity of oral microorganisms, tongue plaque attached to the back of the tongue, and oral diseases such as untreated dental caries and periodontal disease.

It is known that volatile sulfur compounds (VSCs), which are generated by bacterial decay by Gram-negative anaerobic bacteria, account for most of the bad smell in the oral cavity. Among them, bad breath caused by the metabolism of bacteria, which accounts for most of them, is volatile sulfur compounds such as hydrogen sulfide, methyl mercaptan, dimethyl sulfide, n-tetradecanol, phenol, diphenylamine, and pyridine are produced. Non-oral causes of halitosis may include respiratory diseases, neurological gastrointestinal diseases, various other diseases, and use of certain drugs. can occur

On the other hand, among oral diseases, dental caries (cavities) and periodontal disease cause various clinical symptoms such as pain, masticatory dysfunction, destruction of periodontal tissue, and bad breath, and are known as the main factors causing tooth loss. The causative factors of caries are increasing. According to the WHO, more than 60% of adults worldwide have two major oral diseases, dental caries and periodontal disease.

It is a method of inhibiting the growth of oral pathogens that live in the dental plaque and cause dental caries, periodontal disease and bad breath. It has been developed as an inhibitor and therapeutic agent for apical infection.

However, since antibiotics can cause the appearance of resistant bacteria in the oral cavity and mycosis mycosis along with systemic side effects on our body, long-term use is difficult, so there is a disadvantage that it can be used only as a therapeutic agent. In addition, in the case of fluoride, an antibacterial agent, the caries prevention effect is clear, but the effect on periodontal, pulp, and apical diseases has not been reported yet. In addition, antibacterial agents used in toothpaste, mouthwash, and other mouthwashes include saengguinarine, listerine, pyroxide, and chlorhexidine. The effect is not clear and the price is expensive. Listerine is alcohol as its main ingredient. In fact, it has a temporary effect or a slight bacteriostatic effect in the oral cavity. In addition, piroxide, which is recently added for whitening effect, has a toxic effect on bacteria, but at the same time, it also has a toxic effect on human tissues, causing safety problems and sometimes bacteria resistant to pyroxide. .

In addition, Chlorhexidine is known to be the best known among the agents known to date as a preventive and therapeutic agent for periodontal disease along with the inhibition of plaque formation. There are other disadvantages that cannot be used for a long period of time for the purpose of treatment or prevention, such as mycoliosis can be induced, and its use in pregnant women is limited due to its carcinogenicity (Contemporary periodontics [ed. RJ Genco et al.], p161-169, p368-369, p433, CV Mosby Company, St. Louis, 1990).

Accordingly, the present inventors conducted research on various plants in order to derive a natural material having an antibacterial effect against oral pathogens and an effective halitosis suppression effect without fear of side effects as described above.

KR 10-0637653 B1

It is an object of the present invention to provide a composition for alleviating bad breath comprising a seaweed extract as an active ingredient.

Another object of the present invention is to provide a composition for alleviating bad breath using a seaweed extract, which exhibits excellent antibacterial activity against oral microorganisms (pathogens) and an effect of reducing bad breath and at the same time has human stability.

In order to achieve the above object, the composition for alleviating bad breath according to an embodiment of the present invention includes a green laver extract and a seaweed extract.

The composition further comprises a dulce extract, an extract of porcini serrata, and an extract of stony fern.

The extract is extracted using an extraction solvent selected from the group consisting of water, C ₁ to C _{6 lower alcohols, and mixtures thereof.}

The extract is Lactobacillus casei (Lactobacillus casei), Lactobacillus rhamnosus (Lactobacillus rhamnosus), Bifidobacterium bifidium (Bifidobacterium bifidum) Bifidobacterium, Bifidobacterium breve (Bifidobacterium breve) It is fermented with one or more selected from the group consisting of Bacillus acidophilus.

A toothpaste according to another embodiment of the present invention is prepared using a composition for alleviating bad breath including the seaweed extract as an active ingredient.

Gargle liquid according to another embodiment of the present invention is prepared by using a composition for alleviating bad breath including the seaweed extract as an active ingredient.

The oral spray according to another embodiment of the present invention is prepared using a composition for alleviating bad breath including the seaweed extract as an active ingredient.

Hereinafter, the present invention will be described in more detail.

As used herein, the term 'extract' has the meaning commonly used as a crude extract in the art as described above, but in a broad sense also includes a fraction obtained by additionally fractionating the extract. That is, the plant extract includes not only those obtained using the above-described extraction solvent, but also those obtained by additionally applying a purification process thereto. For example, a fraction obtained by passing the extract through an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (prepared for separation according to size, charge, hydrophobicity or affinity), etc. Fractions obtained through various purification methods are also included in the plant extract of the present invention.

The extract used in the present invention may be prepared in a powder state by an additional process such as distillation under reduced pressure and freeze-drying or spray-drying.

In order to achieve the above object, the composition for alleviating bad breath according to an embodiment of the present invention includes a green laver extract and a seaweed extract.

Seaweed contains a large amount of protein and dietary fiber useful for health, but has a lipid content of less than 1%. It is a very good food resource. In addition, seaweed contains a large amount of chlorophyll, carotenoid and phycobilin pigments, which provide color to food, and contain natural antioxidants such as polyphenol compounds, which have been reported to exhibit radical scavenging effects and anti-aging and carcinogenic effects. .

However, as shown in most food resources, there are differences in the content of nutrients and functional ingredients as well as the physiological activity depending on the variety and type of laver. It is effective in calming down.

Pirae laver literally means laver made from green seaweed (Enteromorpha). Plants belonging to the genus Green algae are plants belonging to the green algae family Chrysanthemum, E. linza, E. intestinalis, E. compressa, E. prolifera, or E. clathrata. ), etc. may be included, and preferably green leaves. Most of the plants of the genus Parsley have a cylindrical frond branched or monotonous, and depending on the type, the upper part may be spread into a frond, and taxonomically, a hollow cylindrical type is included in this genus. They mainly grow in the upper intertidal zone of the seashore, especially where fresh water flows, and often form large colonies in quiet tidal pools. It mainly breeds from late autumn to early summer, and is used as food in Korea and Japan.

The leaf green algae (Enteromorpha linza) is a green algae belonging to the end of the green algae plant, and the frond is an elongated and flat frond, monotonous, and the lower part may have some branches. It is widely distributed all over the world, and in the case of Korea, it is distributed all over the coast, and mainly inhabits rocks in the upper intertidal zone or woodwork in bays. In addition, green green leaves are mainly used for food and are also used as kimchi or soup.

Seaweed (Undaria pinnatifida) is a brown algae of the seaweed family that lives in the sea and is similar to a plant, but belongs to a protozoan, not a plant, in classification. It is edible because it contains minerals, vitamins and fiber components, viscous polysaccharides, and iodine. It is a food mainly used in Northeast Asia such as China, Japan, and Korea. Korean records show that it was eaten even in the 12th century, the Goryeo Dynasty, and Chinese records show that Koreans were already eating it in the 8th century. was edible. Dried seaweed contains 12.7% protein, 52% carbohydrate, fat, 1.1%, Ca 1,300mg%, vitamin A 470IU, B1 0.11mg%, B2 0.14mg%, C15mg%, nicotinic acid 10mg%. Silver alginic acid is secreted from the mucous glands and is contained more in leaves and stems than in roots. Polysaccharides in seaweed are not digested or absorbed by the human body and are excreted as they are, so they are not used as a calorie source. On the other hand, algins have strong metal absorption and are effective in removing harmful metals. It is a good source of nutrients as it contains iron, zinc, iodine, potassium and calcium as trace minerals. Therefore, seaweed is a food material that nutritionally shows vitamin and mineral source, intestinal function, alkaline food, removal of harmful metals, anti-cholesterol effect, and the like.

When the green seaweed extract and seaweed extract are used as a composition for alleviating bad breath, the active ingredients contained in the green seaweed extract and seaweed extract exhibit antibacterial activity against oral microorganisms (pathogens) and reduce bad breath and at the same time ensure human safety this is excellent

The composition further comprises xylitol, peppermint essential oil, vegetable glycerin, an abrasive and a thickener.

The xylitol is a sugar alcohol-based natural sweetener used in chewing gum, confectionery, medicine, oral hygiene, and the like. Xylitol is effective in preventing tooth decay because it has a pentose structure. Dental caries is a phenomenon in which the lactic acid produced by the bacteria mutans or sorbinus from eating glucose or fructose in food corrodes the surface of the teeth. can not do it. As a result, acid, the cause of tooth decay, is not generated, and the bacteria that do not receive nutrients are eventually removed from the tooth surface. In addition, xylitol has a refreshing effect that cools the mouth, so it is suitable for preventing tooth decay, such as promoting saliva secretion, so it is used as a caries prevention additive.

The vegetable glycerin is also called glycerol and has a sweet taste in the form of a transparent syrup. Glycerin prevents moisture evaporation and helps supply moisture. In addition, glycerin is harmless to the human body, has excellent moisturizing effect, and has strong moisture absorption. Vegetable glycerin is a natural product made from palm oil and palm oil. As a component that is easily soluble in water, it has an effect of suppressing dermatitis and, in particular, can prevent dry mouth when used in the oral cavity.

In the peppermint essential oil, peppermint (Mentha piperita) has menthol and menthone as components, and is effective in treating itching or inflammation, and is known to be effective in antiseptic, anticonvulsant and expectorant. .

In particular, the anticonvulsant action of peppermint is based on the action on the nervous system, and the fresh and spicy menthol flavor awakens the nerves and brain and improves concentration. It has a local anesthetic effect on muscle pain, back pain, bruises, joint pain, and toothache. In addition, it is one of the most effective oils for the digestive system and is effective for indigestion, motion sickness, stomach pain, diarrhea and flatulence. It constricts blood vessels to speed up the flow of blood vessels to the spleen and promotes blood cell regeneration activity. In addition, by contracting and relaxing the lymph and fascia, it enhances the smooth toxin discharge function and helps relieve headaches and migraines.

The thickener is for maintaining a constant viscosity, and may include a thickener of a natural component. The thickener is selected from the group consisting of xanthan gum, natural cellulose, high cell, methylcellulose, carbomer, carbopol pregel and gelatin, and more specifically, xanthan gum, but is not limited to the above example.

The abrasive may be selected from the group consisting of baking soda, calcium carbonate, and mixtures thereof, and precipitated calcium carbonate may be used as an embodiment of the calcium carbonate. such as baking soda; calcium carbonate; Alternatively, precipitated calcium carbonate can remove plaque adhering to the tooth surface.

Preferably, the composition for alleviating bad breath of the present invention further comprises a dulce extract, an oleifera extract and a stone garfish extract in addition to the green laver extract and seaweed extract.

Polysaccharides contained in seaweed are attracting attention as various physiologically active substances due to their molecular structural characteristics as well as various physiological functions.

The dulce (Palmaria palmata) is a red algae of the order Diptera and grows on the northern coasts of the Atlantic and Pacific Oceans. Dulce is rich in protein, calcium, magnesium and iron, and has long been eaten by people on the coast of Europe. In particular, Dulce has a soft texture and strong flavor, so it is mainly used in soups and salads.

Gracilaria verrucosa (Hudson) Paperfuss is a seaweed in the red algae family, and it is called Koshirak in Jangheung, Wando, Haenam, and Jindo. Stems with a diameter of 1-3 mm usually grow about 20 cm and grow to a maximum of 2-3 m. The body is gathered from small tray-shaped roots and is cylindrical in shape. It is closely divided into pinnates, and the branches are sometimes deviated to one side. Branches extending from the body are wire-shaped with a diameter of 1~2mm and are blackish purple or dark brown. It lives attached to stones and shells in the intertidal zone. It especially breeds on pebbles or stakes in shallow beaches where rivers flow into the sea, and also grows on reefs in the open sea. Especially in the inner bay, it forms a large colony, and there are sometimes very large individuals where fresh water flows. The trunk and branches away from their habitat can wander anywhere in the sea, and the rapid growth between them makes them larger than normal adjoining plants. It is edible or mixed with agar agar to make agar. Collected items are dried and stored. When blanched in hot water, the red pigment, phycopyrin, is destroyed, and only the green pigment, chlorophyll, remains and turns green. The season is from early spring to late autumn. It has low fat and carbohydrate content, contains a large amount of calcium and dietary fiber, and is said to have the effect of excreting heavy metals from the body.

Stonefish (Chondracanthustenellus), also called a lagoon, is mainly distributed in the western part of the North Pacific Ocean and is a seaweed in the family Stonefish family. It lives on rocks in the lower part of the intertidal zone, is 5-12 cm high, and has cylindrical branches that alternate or face each other several times. The branches are pointed. Branches are 1-2 mm wide at the center. The color is purple or reddish-purple, and it fluoresces in water and is a little hard cartilage. It is often used as a mixed raw material in the manufacture of agar. Widely distributed throughout the coasts of Korea and Japan.

A composition for alleviating bad breath is provided by mixing the green laver extract and the seaweed extract with the dulce extract, the seaweed extract and the stone garfish extract, and compared to the case of using only the green seaweed extract and the seaweed extract according to the synergistic effect of the mixed use, oral microorganisms It is possible to equal or increase the excellent antibacterial activity and the effect of reducing bad breath against (pathogens), and further provide a composition with high palatability.

The extract is extracted using an extraction solvent selected from the group consisting of water, C ₁ to C _{6 lower alcohols, and mixtures thereof.}

Specifically, pulverizing seaweed to prepare the seaweed extract; leaching the pulverized product using an organic solvent; drying the sample after leaching; re-leaching the dried sample using an organic solvent; drying the sample after leaching; leaching with water; and leaching to obtain a seaweed extract.

The method may further include fractionating the seaweed extract extracted using the organic solvent using an organic solvent.

The method for preparing the extract may be a conventional extraction method in the art, such as an ultrasonic extraction method, a leaching method, and a reflux extraction method. Specifically, it may be an extract obtained by extracting a natural product from which foreign substances have been removed by washing and drying with water, an alcohol having 1 to 6 carbon atoms, or a mixed solvent thereof, and may be an extract extracted by sequentially applying the solvents to the sample.

The reflux extraction method is water, based on 100 mL of an alcohol having 1 to 6 carbon atoms, 10 to 30 g of a pulverized product of a natural product, a reflux time of 1 to 3 hours, and 50 to 100% alcohol or water having 1 to 6 carbon atoms. More specifically, based on 100 mL of an alcohol having 1 to 6 carbon atoms or 100 mL of water, 10 to 20 g of a pulverized product of a natural product, a reflux time of 1 to 2 hours, and 70 to 90% of an alcohol or water having 1 to 4 carbon atoms. .

The leaching method is performed at 15 to 30° C. for 24 to 72 hours, and water or 50 to 100% alcohol having 1 to 6 carbon atoms is used as an extraction solvent. More specifically, the process is carried out at 20 to 25° C. for 30 to 54 hours, and the extraction solvent is water or 70 to 80% alcohol having 1 to 6 carbon atoms.

In the ultrasonic extraction method, the reaction is carried out at 30 to 50° C. for 0.5 to 2.5 hours, and the extraction solvent is water or 50 to 100% alcohol having 1 to 6 carbon atoms. Specifically, extraction is performed at 40 to 50° C. for 1 to 2.5 hours, and the extraction solvent is water or 70 to 80% alcohol having 1 to 6 carbon atoms.

The extraction solvent may be used in an amount of 2 to 50 times, more specifically, 2 to 20 times, based on the weight of the sample. For extraction, the sample may be left for 1 to 72 hours for leaching in the extraction solvent, and more specifically, for 24 to 48 hours.

After extraction, the extract may be fractionated by sequentially applying a fresh fractionation solvent. The fractionation solvent used for fractionation is at least one selected from the group consisting of water, hexane, butanol, ethyl acetic acid, ethyl acetate, methylene chloride, and mixtures thereof, preferably ethyl acetate or methylene chloride.

After obtaining the extract or fraction, a method such as concentration or freeze-drying may be additionally used.

The extract is Lactobacillus casei (Lactobacillus casei), Lactobacillus rhamnosus (Lactobacillus rhamnosus), Bifidobacterium bifidium (Bifidobacterium bifidum) Bifidobacterium, Bifidobacterium breve (Bifidobacterium breve) It is fermented with one or more selected from the group consisting of Bacillus acidophilus.

More specifically, the composition for alleviating bad breath includes a fermented green seaweed extract, a fermented seaweed extract, a fermented Dulce extract, a fermented fermented serrata extract, and a fermented stone kale extract.

In the case of using the fermented green laver extract, fermented seaweed extract, fermented dulce extract, fermented serrata extract, and fermented stone garfish extract, there is an advantage that it can be used stably at a relatively high concentration due to low cytotoxicity, You can increase your preference.

In addition, in the case of fermentation, it may be advantageous to obtain an active ingredient that has an effect of reducing and alleviating bad breath.

The microorganisms used for the fermentation include Lactobacillus salivarius, Lactobacillus acidophilus, Lactobacillus brevis, Lactobacillus rhamnosus, Lactobacillus rhamnosus, Lactobacillus Lactobacillus plantarum), Lactobacillus helveticus, Lactobacillus fermentum, Lactobacillus paracasei, Lactobacillus casei, Lactobacillus casei, Lactobacillus casei, Lactobacillus Lactobacillus reuteri, Lactobacillus buchneri, Lactobacillus gasseri, Lactobacillus johonsonii, Lactobacillus kefir, such as Lactobacillus johonsonii, Lactobacillus kefir, etc. Lactococcus lactis, Lactococcus plantarum, Lactococcus raffinolactis, Enterococcus faecalis, Enterococcus faecium, Enterococcus faecinium Lactic acid coxae and Bifidobacterium animals such as Streptococcus thermophilus, Leuconostoc lactis, Leuconostoc mesenteroides, etc., Bifidobacterium bifidium (Bifidobacterium bifidum), Bifidobacterium brev (Bifidobact) erium breve), Bifidobacterium infantis, Bifidobacterium longum, Bifidobacterium pseudolongum, Bifidobacterium themophilum, Bifidobacterium Bifidobacterium such as Bifidobacterium adolescentis and the like may be included, and more preferably Lactobacillus casei, Lactobacillus rhamnosus, Bifidobacterium bifidum) may be at least one selected from the group consisting of Bifidobacterium, Bifidobacterium breve, Bifidobacterium, and Lactobacillus acidophilus.

Preferably, the composition is based on 100 parts by weight of the fermented green seaweed extract and 100 parts by weight of the seaweed extract, 30 to 40 parts by weight of the fermented dulce extract, 30 to 40 parts by weight of the fermented serrata extract, and 30 to 40 parts by weight of the fermented rockfish extract. can In the case of the above range, a synergistic effect of critical significance is expressed as a synergistic effect due to the interaction of each extract, and when out of the above range, the synergistic effect is rapidly reduced or almost absent.

In addition, there is no problem of side effects even if the dosage and duration of administration are increased with a food composition of a naturally derived material.

More specifically, the composition is based on 100 parts by weight of the fermented green seaweed extract and 100 parts by weight of the fermented seaweed extract, 30 to 40 parts by weight of the fermented Dulce extract, 30 to 40 parts by weight of the fermented fermented seaweed extract, and 30 to 40 parts by weight of the fermented stone gaseous extract. , 20 parts by weight of xylitol, 10 parts by weight of glycerin, 0.1 parts by weight of peppermint essential oil, 10 parts by weight of glycerin, 10 parts by weight of calcium carbonate, and 7 parts by weight of xanthan gum.

A toothpaste according to another embodiment of the present invention is prepared using a composition for alleviating bad breath including the seaweed extract as an active ingredient.

When the composition for alleviating bad breath is used as a toothpaste, the composition may be added to toothpaste as it is, or mixed or added with a functional food or functional ingredient, and may be appropriately used according to a conventional method. The mixing amount of the active ingredient may be suitably determined according to the purpose of use.

Gargle liquid according to another embodiment of the present invention is prepared by using a composition for alleviating bad breath including the seaweed extract as an active ingredient.

The gargling liquid is defined as including all of the composition capable of gargling the oral cavity and other agents for exhibiting cleaning and antibacterial activity in the oral cavity. On the other hand, the gargle solution for achieving the object of the present invention should be an oil-based composition, and it is preferable not to mix a surfactant or an alcohol. The specific formulation is defined as including both the composition and mixing range that can be selected and mixed by a person skilled in the art.

The oral spray according to another embodiment of the present invention is prepared using a composition for alleviating bad breath including the seaweed extract as an active ingredient.

According to the composition for alleviating bad breath of the present invention, a seaweed extract having excellent human safety while exhibiting antibacterial activity against oral microorganisms (pathogens) and a reduction in bad breath using a seaweed extract containing a green laver extract and a seaweed extract is used as an active ingredient It is possible to provide a composition for alleviating bad breath comprising a.

1 is a graph showing the antibacterial activity against bad breath causative bacteria according to a composition for alleviating bad breath comprising a seaweed extract according to an embodiment of the present invention.

Hereinafter, embodiments of the present invention will be described in detail so that those of ordinary skill in the art can easily carry out the present invention. However, the present invention may be embodied in many different forms and is not limited to the embodiments described herein.

[Preparation Example 1: Preparation of seaweed extract]

1. Preparation of green laver extract

Green seaweed was pulverized with a mixer and then made into powder. 50% ethanol as an extraction solvent was added to the powder sample at a ratio of 1;10 (w; v), and then completely immersed, and extracted three times for 3 hours while refluxing at 80°C. The extract is Whatman No. 2 filtered with filter paper. The filtrate was concentrated under reduced pressure at 60° C. to prepare green laver extract (PE).

2. Preparation of other seaweed extracts

Seaweed extract (UE), dulce extract (DE), seaweed extract (SE), and stonefish extract (CE) were prepared by using the same method as the preparation method of the green laver extract (PE).

3. Preparation of fermented green laver extract

A fermented green laver extract was prepared to enhance palatability with excellent flavor and taste while exhibiting antibacterial activity against the bacteria causing bad breath and reducing bad breath. DW 75% (w/w) of 'Lactobacillus casei', a microorganism used for fermentation, 15% (w/w) Ginseng powdered milk, and 10% (w/w) green seaweed extract (PE) are mixed After inoculation in the sterilized medium and cultured (25 ℃, 24 hours) to prepare a fermented green laver extract (PLc).

4. Preparation of other fermented seaweed extracts

Fermented seaweed extract (ULc), fermented dulce extract (DLc), fermented seaweed extract (SLc), and fermented stone garfish extract (CLc) were prepared using the same method as the preparation method of the fermented green laver extract (PLc).

[Preparation Example 2: Preparation of a composition for alleviating bad breath]

The green seaweed extract, seaweed extract, dulce extract, mussel extract and stone garfish extract prepared in Preparation Example 1 were prepared as a composition for alleviating bad breath with the composition shown in Table 1 below.

HC1 HC2 HC3 HC4 HC5 HC6 HC7 HC8 HC9 HC10 UE 100 100 100 100 100 - - - - - PE 100 100 100 100 100 - - - - - DE - 20 30 40 50 - - - - - SE - 20 30 40 50 - - - - - CE - 20 30 40 50 - - - - - ULc - - - - - 100 100 100 100 100 PLc - - - - - 100 100 100 100 100 DLc - - - - - - 20 30 40 50 SLc - - - - - - 20 30 40 50 CLc - - - - - - 20 30 40 50

(Including unit weight parts, other components include xylitol 20 parts by weight, glycerin 10 parts by weight, peppermint essential oil 0.1 parts by weight, glycerin 10 parts by weight, calcium carbonate 10 parts by weight, and xanthan gum 7 parts by weight)

[Experimental Example 1: Antibacterial effect]

2 g of the prepared compositions for alleviating bad breath HC1 to HC10 were diluted and suspended in 9 g of each sterilized phosphate buffer solution. The diluted suspension was serially diluted two-fold with BHI (Brain heart infusion) medium, and 1 mL each was injected into a 48-well plate.

And in an agar medium, each of Fusobacterium nucleatum ATCC 25586 and Porphyromonas gingivalis ATCC 33277, which are bacteria causing bad breath, was suspended in BHI medium at 20 ml each, and then the diluted 48-well plate. was additionally injected and incubated at 37°C for 24 hours.

Thereafter, the antibacterial activity was measured as a minimal inhibition concentration (MIC), and as a comparative example, the composition was the same as the composition for alleviating bad breath except that the seaweed extract was not included.

The results are shown in FIG. 1 below.

As shown in FIG. 1 , it was confirmed that the strains of the compositions HC1 to HC10 for alleviating bad breath were significantly reduced compared to the comparative example. It can be seen that it exhibits antibacterial activity against the bad breath causative bacteria, Fusobacterium nucleatum ATCC 25586 and Porphyromonas gingivalis ATCC 33277.

[Experimental Example 2: Effect of reducing bad breath]

1. Bad breath test (BB Checker)

Halitosis was measured using BB Checker (mBA-21, Plustech, Korea), which is a device that measures various bad breath gases in the oral cavity including volatile sulfur compounds (VSC). The BB Checker measurement method is as follows.

① Press the OG (Oral Gas) button, and a 180-second pre-preparation countdown starts on the screen. ② When the countdown has about 30 seconds left, take out the probe and insert it into the Mouth piece. ③ Pre-preparation When the countdown starts, hold the probe covered with a paper tube in your mouth. ④ Explain to breathe through the nose for 15 seconds and stop mouth breathing. ⑤ When the measurement is finished, read and record the measurement value output by automatic printing.

Using BB Checker, each of the compositions HC1 to HC10 for alleviating bad breath used in the experiment and before brushing teeth were measured 1 hour after use. Reading criteria were interpreted as normal (0-50 BBV), slightly halitosis (50-70 BBV), moderately severe symptom management (70-90 BBV), and severe bad breath (90-100 BBV).

The experiment was conducted on 20 people, and measurements were carried out before and 1 hour after using the composition for alleviating bad breath. In order to increase the accuracy of the measurement, the same meal is provided to the same person after 6 hours, and the results measured before and 1 hour after brushing using the compositions HC1 to HC10 for alleviating bad breath of the present invention are shown below. Table 2 shows.

before brushing 1 hour later HC 1 #One 44.70±13.87 47.50±21.86 #2 33.60±9.66 30.20±8.75 HC 2 #One 35.17±14.51 35.50±8.15 #2 29.00±8.95 28.33±9.44 HC 3 #One 33.40±6.17 32.20±8.38 #2 29.70±7.53 27.40±10.21 HC 4 #One 34.00±5.87 33.20±8.38 #2 31.70±7.63 29.50±11.21 HC 5 #One 28.20±6.87 28.20±6.38 #2 31.70±7.63 29.25±11.21 HC 6 #One 38.00±5.87 39.20±1.38 #2 36.70±5.63 33.50±9.21 HC 7 #One 33.21±5.87 30.20±4.38 #2 32.70±5.63 28.50±3.21 HC 8 #One 40.20±5.87 34.20±3.38 #2 33.70±5.63 28.50±4.21 HC 9 #One 38.00±4.87 30.21±3.38 #2 32.70±4.63 27.50±3.21 HC 10 #One 33.21±5.87 31.20±5.38 #2 32.62±4.63 29.50±4.21

According to Table 2, it was confirmed that HC2 to HC5 had an excellent effect of reducing bad breath compared to HC1 containing only the green laver extract and seaweed extract. In particular, in the case of mixing in the range of HC8 to HC9 containing the fermented seaweed extract, the effect of reducing bad breath was further increased.

Therefore, in the case of the composition for alleviating bad breath in the range of HC8 and HC9 containing the fermented seaweed extract, only the fermented green laver extract and the fermented seaweed extract (HC6) are used as a composition for alleviating bad breath due to the synergistic effect of mixing each extract. It can be seen that there is a synergistic effect that is more than a simple combination.

[Experimental Example 3: Sensory evaluation]

HC3, HC4, HC8, and HC9 compositions for alleviating bad breath were randomly assigned to 30 people and divided into 15 groups, the first group was given a composition for alleviating bad breath of HC3 and HC4, and the second group was HC8 and HC9. After the composition for alleviating bad breath of

The results are shown in Table 4.

Survey content 1. Less dry mouth 2. Feel refreshed 3. The mouth is not sticky. 4. It feels like the color of teeth has become brighter. 5. Gum inflammation seems to have improved. 6. It seems that the surface of the teeth has become smooth. 7. It seems to be effective for teeth whitening.

Survey 1 Survey 2 Survey 3 Survey 4 Survey 5 Survey 6 Survey 7 1st Army HC3 7 7 8 8 8 5 8 HC4 8 7 8 9 10 4 7 2nd Army HC8 9 10 7 8 10 7 8 HC9 10 8 8 9 10 6 9

(Unit: Index)

Table 4 shows the evaluation scores performed for the questionnaire contents as in Table 3, and the higher the score, the better the evaluation.

The first group and the second group were distinguished according to whether the ferment extract was used in the composition for alleviating bad breath, and the sensory evaluation result was performed. A better evaluation was confirmed.

Although the preferred embodiment of the present invention has been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements by those skilled in the art using the basic concept of the present invention as defined in the following claims are also provided. is within the scope of the

Claims (7)

It contains fermented green laver extract and fermented seaweed extract as active ingredients,
To further include fermented dulce extract, fermented fermented serrata extract, fermented rockfish extract, xylitol, glycerin, peppermint essential oil, calcium carbonate and xanthan gum,
With respect to 100 parts by weight of the fermented green seaweed extract and 100 parts by weight of the fermented seaweed extract, 30 to 40 parts by weight of a fermented dulce extract, 30 to 40 parts by weight of a fermented serrata extract, 30 to 40 parts by weight of a fermented stone gas extract, and 20 parts by weight of xylitol , 10 parts by weight of glycerin, 0.1 parts by weight of peppermint essential oil, 10 parts by weight of calcium carbonate, and 7 parts by weight of xanthan gum
A composition for alleviating bad breath comprising a seaweed extract as an active ingredient. delete The method of claim 1,
The extract is extracted using an extraction solvent selected from the group consisting of water, C ₁ to C _{6 lower alcohols and mixtures thereof.}
A composition for alleviating bad breath comprising a seaweed extract as an active ingredient. The method of claim 1,
The extract is Lactobacillus casei (Lactobacillus casei), Lactobacillus rhamnosus (Lactobacillus rhamnosus), Bifidobacterium bifidium (Bifidobacterium bifidum) Bifidobacterium, Bifidobacterium breve (Bifidobacterium breve) Bifidobacterium, and Lactobacillus Bacillus acidophilus (Lactobacillus acidophilus) that is fermented with one or more selected from the group consisting of
A composition for alleviating bad breath comprising a seaweed extract as an active ingredient. A composition comprising the composition for alleviating bad breath according to claim 1
toothpaste. A composition comprising the composition for alleviating bad breath according to claim 1
gargle liquid. A composition comprising the composition for alleviating bad breath according to claim 1
oral spray.

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