KR101978365B1 - Composition for preventing or alleviating periodontal disease comprising porphyra tenera extract - Google Patents

Abstract

The present invention relates to a composition for preventing and treating periodontal disease containing an extract of Porphyra tenera, which has an excellent anti-inflammatory effect against inflammation of human gingival fibroblast-1 (HGF-1) by containing the extract of Porphyra tenera. In addition, unlike existing antibiotics, the composition uses an extract of marine algae and the like, to be used for edible purposes, thereby having no problems of resistance and side effects and being able to be provided as a toothpaste, an oral spray, an oral cleanser, a chewing gum and the like which have excellent preference.

Description

TECHNICAL FIELD [0001] The present invention relates to a composition for preventing and treating periodontal disease,

The present invention relates to a composition for the prevention and treatment of periodontal disease including a Kim extract. More specifically, the present invention relates to a composition for preventing and treating periodontal disease, which can alleviate inflammation of gingival fibroblast (HGF-1, Human Gingival Fibroblast-1).

Periodontal tissue consists of alveolar bone, gingiva (gums) and periodontal ligament. The gingiva is a part of the tooth supporting tissue that we can see in the mouth. The disease (gingivitis) starts here. When the disease deepens and spreads deeply into the supporting tissue, the periodontal ligament attached to the bone tissue surrounding the tooth root The ligament is destroyed and the alveolar bone surrounding the tooth is destroyed, causing periodontal disease.

Periodontal disease, including gingivitis and periodontal disease, is an inflammatory state of the tooth supporting tissues caused by bacteria, which causes loss of teeth due to bleeding, formation of the periodontal pouch, and destruction of the alveolar bone. This periodontal disease progresses to bacterial colony formation, bacterial infiltration of periodontal tissue, and periodontal tissue destruction.

Specifically, when the oral bacteria are formed due to a poor oral hygiene condition, a bacterial membrane is formed. When the bacteria form, the blood gushes from the gums and the bad breath develops. In this state, the gap between the tooth and the gum becomes deeper, , Which causes bacteria that cause periodontal disease, resulting in periodontitis.

If periodontitis develops, weak stimuli such as toothbrushing may cause bleeding from the gums, swelling, and often acute inflammation leading to pain. These inflammations are caused by the action of collagen, calcium, and phosphorus (hydroxyapatite) to reduce the ability to make bone in osteoblasts that make hard bones, and to destroy or resorb bone in osteoclasts The alveolar bone is gradually lowered and destroyed, eventually resulting in tooth loss.

At this time, it is known that osteoclasts are derived from monocyte / macrophage lineage cells in the bone marrow, and these mononuclear progenitor cells circulate in the blood and progenitor cells are proliferated in the endosteal layer and fused to form polynuclear cells (Scheven, BAA et al., Nature, 321: 79-81, 1986), osteoclasts have a tartrate-resistant acid phosphatase (TRAP), an acid phosphatase characteristic of tartrate resistance, (Minkin, C., Calcif. Tissue Int., 34: 285-290, 1982). It is known to be used as a cytochemical labeling enzyme for osteoclast differentiating from tissue cells.

There are many causes of periodontal disease, but when the plaque accumulates in the periodontal pocket due to local factors, it becomes a habitat of anaerobic Gram negative bacteria present in the surrounding area and proliferates to the deep part of the periodontal pouch. At this time, the toxins and all products of the anaerobic Gram-negative bacteria that are proliferated directly destroy the tissue or stimulate the immune system and stimulate the immune system, resulting in various actions and inflammation along with periodontal tissue destruction. The function and immune response of the polymorphonuclear leukocyte function as a systemic factor before the defense period.

Treatment of periodontal disease has been based on the establishment of improved oral hygiene in patients, non-surgical or surgical calculus removal, root resorption, gingival curettage and periodontal tissue regeneration. However, these surgical treatment methods are difficult to effectively treat due to the inconvenience of going to the dentist for treatment, and they are limited to the treatment that is performed when the disease progresses to some extent rather than to the prevention of the disease. .

The use of systemic antibiotics and local controlled release agents have been used as adjunctive therapies. However, there has been reported a case in which periodontal disease bacteria, which are resistant to antibiotics recently used, There is a serious problem.

In order to overcome the limitations of such surgical treatment and to solve the problems of antibiotic use and manifest the effect of prevention and treatment of periodontal disease, it was necessary to produce a preparation having a function of recovering the damaged and lost periodontal tissue. It is also necessary to develop a therapeutic agent capable of overcoming resistance and safety problems caused by conventional therapeutic agents.

(Patent Document 1) KR 10-1592549 B1

It is an object of the present invention to provide a composition for the prevention and treatment of periodontal disease including a Kim extract.

Another object of the present invention is to provide a composition for the prevention and treatment of periodontal disease which can alleviate inflammation of gingival fibroblast (HGF-1, Human Gingival Fibroblast-1) using a Kim extract.

Another object of the present invention is to provide an oral cleaning composition, oral cleaning agent, dentifrice and food composition containing the composition for preventing and treating periodontal disease.

In order to achieve the above object, the composition for the prevention and treatment of periodontal disease according to one embodiment of the present invention comprises an extract of Kim as an active ingredient and is used as an anti-inflammatory agent for gingival fibroblast (HGF-1, Human Gingival Fibroblast-1) And the effect is excellent.

The Kim extract may be extracted with a solvent selected from the group consisting of water, alcohols having 1 to 6 carbon atoms, and mixtures thereof.

The composition for the prevention and treatment of periodontal disease may be selected from the group consisting of Undaria pinnatifida, Laminaria japonica, Monostroma nitidum Wittrock, Codium fragile (Suringar) Hariot, Codium cylindricum Holmes, ≪ RTI ID = 0.0 > a < / RTI > algae extract.

The composition for the prevention and treatment of periodontal disease may further comprise an extract of Perilla frutescens var. Acuta Kudo.

The composition for the prevention and treatment of periodontal disease may further comprise an extract of Fleas (Arenaria serpyllifolia L.).

The composition for oral care according to another embodiment of the present invention includes the composition for preventing and treating periodontal disease including the extract of Kim.

The oral cleaning agent according to another embodiment of the present invention includes the composition for preventing and treating periodontal disease including the extract of Kimchi.

The toothpaste according to another embodiment of the present invention includes the composition for preventing and treating periodontal disease including the extract of Kimchi.

The composition for food according to another embodiment of the present invention includes a composition for prevention and treatment of periodontal disease including the extract of Kim.

Hereinafter, the present invention will be described in more detail.

The composition for preventing and treating periodontal disease according to one embodiment of the present invention includes Gelidium amansii extract as an active ingredient.

The Kim extract is characterized by having excellent anti-inflammatory effect of gingival fibroblast (HGF-1, Human Gingival Fibroblast-1). Since the anti-inflammatory effect against inflammation of gingival fibroblast, Lt; / RTI >

Porphyra tenera, also known as sea grass, grows on the reef of the sea like moss. Long oval or oval shape with wrinkles on the edges. It is reddish brown and blueish green. The carbohydrate agar is the most abundant and contains hemicellulose, sorbitol and dulcitol. Almost no fat, but 30-40% protein. In particular, it contains many essential amino acids such as threonine valine, leucine, isoleucine, lysine, methionine, phenylalanine and Tryptophan . Glycine and alanine, which are especially sweet and oily amino acids, are contained in this product.

The Kim extract may be obtained by extracting and isolating from nature using extraction and isolation methods known in the art, and the 'extract' defined in the present invention is extracted from Kim using an appropriate solvent, For example, a hot water extract of Kim, a polar solvent-soluble extract or a non-polar solvent-soluble extract

More specifically, the Kim extract is extracted with a solvent selected from the group consisting of water, an alcohol having 1 to 6 carbon atoms, and a mixture thereof. Specifically, the extract may be an extract of Kim extract obtained by extracting ethanol with an extraction solvent. 75% ethanol as an extraction solvent, but it is not limited to the above example.

On the other hand, the above-mentioned Kim extract can be obtained by fermenting an extract after preparing an extract using Kim. When fermenting the above extract, the stability is excellent, the storage stability is improved, and it is advantageously used stably at a relatively high concentration.

Examples of the microorganism used for the fermentation include Lactobacillus salivarius, Lactobacillus acidophilus, Lactobacillus brevis, Lactobacillus rhamnosus, Lactobacillus plantarum Lactobacillus plantus, Lactobacillus plantarum, Lactobacillus helveticus, Lactobacillus fermentum, Lactobacillus paracasei, Lactobacillus casei, Lactobacillus delbrueckii, Such as Lactobacillus reuteri, Lactobacillus buchneri, Lactobacillus gasseri, Lactobacillus johonsonii, Lactobacillus kefir, and the like, lactic acid bacteria such as lactobacillus kefir, Lactococcus lactis, Lactococcus plantarum, Lactococcus raffinolactis, Enterococcus faecalis, Enterococcus faecium, Streptococcus thermophilus, Streptococcus spp. Bifidobacterium bifidum, Bifidobacterium bifidum, Bifidobacterium bifidum, and Bifidobacterium bifidum, such as Leuconostoc lactis, Leuconostoc mesenteroides, and the like, Bifidobacterium species such as Bifidobacterium breve, Bifidobacterium infantis, Bifidobacterium longum, Bifidobacterium pseudolongum, Bifidobacterium themophilum, Bifidobacteria such as Bifidobacterium adolescentis, and the like, Lactobacillus casei, Lactobacillus rhamnosus, Bifidobacterium bifidum bifidobacterium, Bifidobacterium breve bifidobacterium, and Lactobacillus species such as Lactobacillus casei, Lactobacillus rhamnosus, And Lactobacillus acidophilus. ≪ Desc / Clms Page number 2 >

The compositions for prevention and treatment of periodontal disease may further contain other ingredients such as undaria pinnatifida, Laminaria japonica, Monostroma nitidum Wittrock, Codium fragile (Suringar) Hariot, Codium cylindricum, And mixtures thereof. ≪ RTI ID = 0.0 > [0040] < / RTI >

Undaria pinnatifida is an algae belonging to Phaeophyceae Lamaariaceae (Alariaceae). Its base is dendritic, its stem is an eggplant, its upper part is the midrib The leaf has the right upper lobe on the chow. It is distributed in Korea and Japan. It is distributed in a wide area from Baekryeong Island on the west coast to the end of the east coast in Korea. It has been cultivated in the southern coastal areas of Busan and Wando and has been edited in Korea and Japan.

The seaweed (Laminaria japonica) is a seaweed belonging to Phaeophyta, Phaeophyceae, Laminariales, Laminariaceae, and is apparently divided into lobes, stems and roots. The lobes are long, The central part of the foliage forms a thick middle part, and the middle part has a vertical flaw. The stem is usually circumferential and upright, and the root represents the dendritic shape branched from the bottom of the stem. The kelp has been widely used for edible purposes in Japan and China, including Korea.

Monostroma nitidum Wittrock is distributed mainly in the southern coast and Jeju Island in Korea and is distributed in Ulleungdo and Pohang Youngil Bay in the East Sea coast. The body is fragile with lanceolate, irregular and wrinkled. The color of the leaves may be lustrous and lustrous. The size is about 2 to 10 ts, but sometimes there are foliage that grows more than 20 ts. The waves grow in the upper part of the quiet intertidal zone and form a community.

The abovementioned auditory (Codium fragile (Suringar) Hariot) is a species found in the lower part of Korea. The body is upright on the circumference and branch branches more or less uniformly. The young ones have hairs on the front but fall behind. It grows on the rocks of Jodai-dong, which is deep in the intertidal zone. The size is 10 to 30 cm, the thickness is about 1.5 to 3 mm, and the lower part is thicker than the upper part.

Codium cylindricum Holmes is a species found mainly in the southern coast of Korea. It grows in Dokdo, Busan, and other places on the East Sea. The body grows thick and long, and it has the feature of branching in two branches regularly. The body is entirely circumferential, but the branches become flat. Body color is yellowish green and fleshy, and when dried on the earth, the side looks green. It grows in the lower part near the low level. I prefer a quiet place where the influence of the waves is not great, and sometimes the size grows more than 1m and the thickness is about 1cm.

Preferably, it may include a Kim extract, a Chrysanthemum morifolium extract, an auditory extract, and a pillar auditory extract.

The anti-inflammatory effect on the gingival fibroblasts can be increased by the use of the components in combination with the above-mentioned seaweed extract, as compared with the case of using the above-mentioned Kim extract alone.

In order to further enhance the anti-inflammatory effect against gingival fibroblasts, the composition for preventing and treating periodontal disease of the present invention may further comprise a perilla extract (Perilla frutescens var. Acuta Kudo) and a flea venom (Arenaria serpyllifolia L.) Extracts may be further included.

The perilla leaf (Perilla frutescens var. Acuta Kudo) is a cultivated annual plant, 60 to 90 cm high, square, with straight, long hairs. Leaves are larvae, long petiole, ovate, acute ears, long hairs on veins, sawtooth on edge. Flowers are light purple, hanging on branches and main stem at 8 to 9 months. The fruit is in the calyx in the calyx.

The flea spot (Arenaria serpyllifolia L.) is a 1-2 year old weed growing in a sunny place, 10-25cm high, and the main stem divides a lot from the bottom. Leaves are larvae, petiole free, oval or wide oval, and both ends are narrow. The flower is white, and in April to May, the vase comes out from the axil of the leaf and runs on the acupuncture points. Seed is elongate and dark brown.

As a result of further intercalation of the flea weevil extract, which is effective for alleviating periodontal disease, and the lyophilized extract excellent in anti-inflammatory effect, the anti-inflammatory effect against gingival fibroblasts is increased , And as a result, it exhibits superior effects for prevention and treatment of periodontal disease.

Preferably, the composition for preventing and treating periodontal disease comprises a Kim extract, wherein 50 to 80 parts by weight of a chrysanthemum chinensis extract, 30 to 50 parts by weight of a chrysanthemum extract, 30 to 50 parts by weight of a chrysanthemum extract, 20 to 50 parts by weight of the extract, 20 to 50 parts by weight of the flea spot extract. In the above range, a synergistic effect with a critical significance is expressed by the synergistic effect due to the interaction of the respective extracts, and when the concentration is outside the above range, the synergistic effect is significantly lowered or hardly occurs.

The kind of the food of the present invention is not particularly limited. Examples of the health functional food to which the above extraction mixture can be added include meat products, sausages, breads, chocolates, candies, snacks, confectionery, pizza, ramen noodles, other noodles, gums, dairy products including ice cream, , A drink, an alcoholic beverage, and a vitamin complex, and may include foods used as food for animals, which may include all health functional foods in the conventional sense. In addition to the above, the health functional food composition of the present invention may contain various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloid thickening agents, pH adjusting agents, stabilizers, , Alcohols, carbonating agents used in carbonated drinks, and the like. It may also contain flesh for the production of natural fruit juices, fruit juice drinks and vegetable drinks.

The dentifrice composition, mouthwash (goggle) and oral spray of the present invention can be used as a medicinal active ingredient as a dentifrice preventive agent such as sodium fluoride, sodium fluorophosphate, fluorinated amine, tin fluoride or xylitol; Chlorhexidine, cetylpyridinium chloride or triclosan, which are bactericides; Bamboo salt or propolis; Inflammatory emollients such as allantoin, aminocaproic acid, tranexamic acid, tocopherol acetate or methylsulfonylmethane; And one or more components selected from the group consisting of enzymes dextranase, glucose oxidase, glucoamylase,?,? -Amylase, lactoperoxidase and lysozyme.

In addition, the dentifrice composition, oral cleaning agent and oral spray of the present invention may further comprise at least one substance selected from the group consisting of abrasives, wetting agents, binders, foaming agents, sweeteners, anionic surfactants, perfumes and pigments. The form of the composition may be in the form of paste, powder, liquid (goggle type) or powder form.

As described above, when the composition for the prevention and treatment of periodontal disease of the present invention is used as a composition for food or a composition for oral cleanser, there is a problem that the palatability of the seaweed extract may be poor due to its pungent taste and toughness. In addition, the bitter taste of the lobular extract and flea-littion extract may be problematic.

Accordingly, in order to prevent the problem of poor palatability and to further enhance the preventive and therapeutic effect of periodontal disease, the present invention may further include a small amount of Glycyrrhiza uralensis extract and Yellow melilot extract.

The licorice (Glycyrrhiza uralensis) is a root and rootstock of soybean, European licorice, Manchuria licorice, and other herbaceous plants. It is collected in spring and autumn, and the beard roots are removed and sun dried. The nature is plain and the taste is sweet. It has the property of thawing when it is used, and it can treat Changyang, Tonghodok, Throat, and food poisoning. It also has the property of harmonizing the drug to attenuate the quasi-rugged nature of the drug. Abdominal pain, diarrhea, heart disease, heart palpitations, span, and lung can be treated.

The above-mentioned Yellow melilot is also called a melon. It is the northern part of China. It grows in low-lying meadows. It is 60 to 90 cm high and branches are split and smelled when dried. There are hairs in my childhood, but they disappear later. Leaves are alternate phyllotaxis of three small leaves. Leaves are long oval or inverted, and have small sawtooth edges. The flower blooms in July or August and it is yellow, and it runs tightly with gunshot at the tip of the branch or the axilla. Calyx has hairy hairs. The fruit is egg-shaped, has no hair, and is black. The entire paste is used for heat, heat, nephritis, etc. It is called white algae (M. alba) that flowers are smaller and white. Everything that grows as a grass resource grows wild and grows.

The licorice extract and the electric sphygmomanometer extract are contained in a small amount to increase the preference of the composition for the prevention and treatment of periodontal disease and increase the anti-inflammatory effect on the gingival fibroblast by mixing with the constituents.

Preferably, the composition for preventing and treating periodontal disease according to the present invention comprises 50 to 80 parts by weight of a chrysanthemum chinensis extract, 30 to 50 parts by weight of a chrysanthemum extract, 30 to 50 parts by weight of a chrysanthemum extract, 20 to 50 parts by weight of a leaf extract, 20 to 50 parts by weight of a flea spot extract, 10 to 20 parts by weight of a licorice extract, and 1 to 5 parts by weight of an electric sirate extract. In the above range, a synergistic effect with a critical significance is expressed by the synergistic effect due to the interaction of the respective extracts, and when the concentration is outside the above range, the synergistic effect is significantly lowered or hardly occurs.

The composition for prevention and treatment of periodontal disease comprising the extract of Kimchi of the present invention is excellent in the prevention and treatment of periodontal disease which can relieve inflammation of gingival fibroblast (HGF-1, Human Gingival Fibroblast-1).

In addition, unlike existing antibiotics, it can be provided with dentifrices, oral sprays, oral cleansers, chewing gums and the like which have no problem of resistance and side effects and are excellent in preference in that they use algae extract or the like to be used for edible purposes.

BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1 is a graph showing the results of measurement of IL-6 in BGF-1 when the composition for the prevention and treatment of periodontal disease of the present invention is treated. Fig.
Fig. 2 relates to the measurement result of IL-1? In BGF-1 when the composition for the prevention and treatment of periodontal disease of the present invention is treated.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily carry out the present invention. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.

[Preparation Example 1: Preparation of composition for prevention and treatment of periodontal disease]

1. Preparation of Kim extract (PE)

The steaming was washed thoroughly, thoroughly dried, and then pulverized using a pulverizer. After the milling, 100 ml of 75% ethanol was added and extracted at room temperature for one day to prepare an extract.

The extract was filtered, and the obtained filtrate was concentrated under reduced pressure using a vacuum evaporator to prepare a Kim extract.

2. Preparation of other extracts

Seed extracts (ME), auditory extracts (SE), column hearing extracts (HE), leafy leaves extracts (FE) and flea spots extracts (AE) were prepared in the same manner as the above Kim extract.

3. Preparation of compositions for the prevention and treatment of periodontal disease

(PE), chrysanthemum extract (ME), auditory extract (SE), column hearing extract (HE), lobule extract (FE) and flea chrysanthemum extract (AE) A composition for the prevention and treatment of periodontal disease was prepared.

DX1 DX2 DX3 DX4 DX5 DX6 PE 100 100 100 100 100 100 ME 30 50 60 70 80 90 SE 20 30 40 45 50 55 HE 20 30 40 45 50 55 FE 10 20 30 40 50 60 AE 10 20 30 40 50 60

(Unit weight parts)

[Experimental Example 1: Anti-inflammatory effect on inflammatory reaction of gingival fibroblast]

Gingival fibroblasts (HGF-1) were stimulated by treatment with LPS to induce an inflammatory response. HGF-1 was cultured in DMEM medium containing 20% FBS. HGF-1 was dispensed into a 24-well plate at a density of 1.0 × 10 ⁵ per well and cultured overnight.

DX1 to DX6 samples are exchanged with DMEM medium (with 2% FBS) and incubated for 3 hours for pretreatment. LPS (1 ug / mL), cultured for 4 hours, and cytokine (IL-6, IL-1beta) was measured at -80 ° C. UN means no treatment, CON means 1 μg / mL of LPS.

The results are shown in FIGS. 1 and 2.

Referring to FIGS. 1 and 2, it is confirmed that both IL-1β and IL-6 are reduced by the composition of the present invention. Therefore, it can be confirmed that the DX1 to DX6 composition of the present invention has an excellent anti-inflammatory effect against the inflammatory reaction of gingival fibroblast.

[Preparation Example 2: Preparation of mixed composition]

1. Preparation of licorice extract (GE)

The licorice was washed, dried and then pulverized. The pulverized material was mixed with purified water and maintained at 98-100 ° C for 2 hours. After cooling, it was filtered with Watman filter paper to obtain a filtrate.

2. Preparation of Electrolytic Extract (TE)

(TE) was prepared in the same manner as the licorice extract.

3. Preparation of mixed composition

The composition for prevention and treatment of periodontal disease was prepared by mixing licorice extract (GE) and electrorheological extract (TE) with DX4 composition prepared in Preparation Example 1 in the composition shown in Table 2 below.

DX4 TX1 TX2 TX3 TX4 TX5 TX6 PE 100 100 100 100 100 100 100 ME 70 70 70 70 70 70 70 SE 45 45 45 45 45 45 45 HE 45 45 45 45 45 45 45 FE 40 40 40 40 40 40 40 AE 40 40 40 40 40 40 40 GE - 5 10 14 18 20 22 TE - 0.5 One 2 4 5 7

(Unit weight parts)

[Experimental Example 2: Anti-inflammatory effect on inflammatory reaction of gingival fibroblast]

In order to confirm the increase of the anti-inflammatory effect by the interaction of each of the extracts with the mixed composition prepared in Preparation Example 2, the anti-inflammatory effect of the gingival fibroblast was examined by using DX4 The composition and comparative evaluation were carried out.

Experiments were carried out in the same manner as in Experimental Example 1, and an exponent was used to show the results of the comparison test more clearly. That is, cytokine (IL-6, IL-1beta) measurement results of the DX4 composition were fixed at an index of 5, and the results of the use of the mixed composition were expressed as an index of 1 to 10 compared with the results. The higher the number, the better the anti-inflammatory effect of the gingival fibroblast's inflammatory response.

DX4 TX1 TX2 TX3 TX4 TX5 TX6 IL-6
production 5 4 5 8 7 5 6 IL-1?
production 5 5 6 8 6 6 6

(Unit index)

According to the above Table 3, it can be confirmed that the inflammation-reducing effect of the gingival fibroblast is partially improved when the mixed composition is used as compared with the case of using the DX4 composition.

[Experimental Example 3: Sensory Test]

The composition of DX4 prepared in Preparation Example 1 and the composition of TX1 to TX6 prepared in Preparation Example 2 were subjected to a sensory test when used as an oral cleanser.

10% by weight of each of the compositions of DX4 and TX1 to TX6 was mixed with 90% by weight of water to prepare an oral cleanser. Ten adult male and female subjects were then subjected to a sensory evaluation. The sensory evaluation was evaluated by an index of 1 to 10 according to a refreshing sensation, a residual sensation, a clear taste, and a stimulus.

DX4 TX1 TX2 TX3 TX4 TX5 TX6 Refreshing feeling 6 7 7 8 8 8 6 Residual feeling 5 6 6 7 8 5 4 A tasty taste 8 5 5 3 2 3 One Stimulation One One One One One One One

(Unit index)

According to the results shown in Table 4, DX4 may have poor taste due to the taste of mouth as an oral cleanser.

On the other hand, in the case of TX1 to TX6, the taste is remarkably improved as compared with DX4, and the preference for oral cleanser can be enhanced.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, Of the right.

Claims (9)

Kim (Porphyra Tenera) extract as an active ingredient,
Gingival fibroblasts (HGF-1, Human Gingival Fibroblast-1) have excellent anti-inflammatory effects,
Addition of algae extracts selected from the group consisting of undaria pinnatifida, Laminaria japonica, Monostroma nitidum Wittrock, Codium fragile (Suringar) Hariot, Codium cylindricum Holmes and mixtures thereof As including
A composition for the prevention and treatment of periodontal disease, comprising a Kim extract. The method according to claim 1,
The Kim extract is extracted with a solvent selected from the group consisting of water, an alcohol having 1 to 6 carbon atoms, and a mixture thereof
A composition for the prevention and treatment of periodontal disease, comprising a Kim extract. delete The method according to claim 1,
The composition for the prevention and treatment of periodontal disease may further comprise a Perilla frutescens var. Acuta Kudo extract
A composition for the prevention and treatment of periodontal disease, comprising a Kim extract. The method according to claim 1,
The composition for the prevention and treatment of periodontal disease further comprises an extract of Fleas (Arenaria serpyllifolia L.)
A composition for the prevention and treatment of periodontal disease, comprising a Kim extract. A composition for prevention and treatment of periodontal disease comprising the extract of Kimchi according to claim 1
Compositions for oral cleansing. A composition for prevention and treatment of periodontal disease comprising the extract of Kimchi according to claim 1
Oral cleanser. A composition for prevention and treatment of periodontal disease comprising the extract of Kimchi according to claim 1
toothpaste. A composition for prevention and treatment of periodontal disease comprising the extract of Kimchi according to claim 1
Food composition.

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