KR101703269B1 - Oral care composition comprising Magnolia officinalis extract and bicarbonate - Google Patents

Abstract

The present invention relates to a composition for oral care comprising a roast extract and a bicarbonate, and relates to a composition exhibiting excellent antimicrobial effect by the synergistic effect of roast extract and bicarbonate. The oral care composition of the present invention exhibits excellent effects in removing bad breath and preventing periodontal disease.

Description

[0001] The present invention relates to a composition for oral care comprising Magnolia officinalis extract and bicarbonate,

The present invention relates to a composition for oral care, and more particularly to a composition for oral care having excellent antibacterial effect, bad breath removing effect, periodontal disease prevention effect and the like.

Bad breath is caused by the decomposition of amino acids generated by microorganisms in the oral cavity by proteins, food drips, and the like, which are present in the saliva, by a demineralization enzyme or an amino enzyme to generate a malodorous substance. These volatile compounds include hydrogen sulfide, methimercaptan, dimethyl mercaptan and the like. In addition to these volatile sulfides, volatile amine compounds such as triethanolamine, aldehyde, fatty acid, and ammonia are also included.

One of the causes of bad breath is called a jig or cheek, which refers to a chunk before it is calcified to a microbial substrate such as a compact, gel and gel fixed to the tooth surface with bacteria and its by-products and food residues in the oral cavity. These plaques are known to be the most important factors in oral diseases because they cause tartar formation due to calcification and also cause inflammatory periodontal disease.

Therefore, in order to suppress the bad breath, plaque and inflammation caused by the oral cavity, it is the most important factor to prevent the bad breath caused by plaque by blocking the bad breath inducing substance by removing the microorganisms in the oral cavity.

As interest in natural extracts has increased recently, studies on various plants have been actively conducted. As the effects on oral cavity are known, many extracts have been applied to oral compositions related to halitosis suppression and prevention of gum disease.

Korean Patent Laid-Open Publication No. 2001-0036152 discloses a method for preparing a plant extract powder and an oral composition containing a plant extract powder prepared by the method, wherein a medicinal plant extract, which is widely known in the arts than artificial antibiotics and fungicides, In particular, it is disclosed that pine needle can be used to provide excellent periodontal disease and tooth decay prevention effect in the oral cavity.

Korean Patent Laid-Open Publication No. 2002-00087672 has an excellent effect for eliminating bad breath due to antibacterial activity and preventing gum disease as well as providing a refreshing sensation in mouth gagging because it contains clove extract and has an analgesic effect which is not possessed by conventional detergents The fact that it has been disclosed.

Korean Patent Laid-Open Publication No. 2003-0000275 relates to a method for producing toothpaste by green tea extract, wherein the green tea extract is excellent in antibacterial action and cavity function of the main component catechin component, and is excellent in removing the mouth odor in the mouth And thus it has been demonstrated that it is excellent as a bad breath removing agent.

As described above, many plant extracts have been used for the purpose of promoting oral hygiene, but in most cases, they have weak antimicrobial effect, so that it is difficult to use oral compositions as a preventive effect of halitosis and periodontal disease.

SUMMARY OF THE INVENTION Accordingly, the present invention has been made to solve the above-mentioned problems and to provide a composition for oral care having an excellent antibacterial effect.

Further, the present invention suppresses the growth of anaerobic bacteria causing oral diseases.

In addition, the present invention aims to provide a composition for oral care which is excellent in the effect of removing bad breath.

The oral care composition of the present invention is intended to provide oral health and periodontal disease, in particular prevention and treatment of gingivitis.

In order to solve the above-mentioned problems, the present invention provides a composition for oral care comprising a rosemary extract and a bicarbonate, preferably a composition for oral care that exhibits an excellent antimicrobial effect through synergistic effects when the rosemary extract and bicarbonate are used together .

The bicarbonate may include sodium hydrogencarbonate, potassium hydrogencarbonate, magnesium hydrogencarbonate and the like, preferably sodium hydrogencarbonate, potassium hydrogencarbonate or a mixture thereof.

In other words, the inventors of the present invention found that the antimicrobial effect is enhanced by the synergistic effect between sodium hydrogencarbonate and sucrose extract when the crude extract and sodium hydrogencarbonate, which are natural extracts having excellent antimicrobial activity with little safety or irritation, are used together Respectively. The present inventors have completed the present invention by confirming that they have an excellent action in terms of reducing bad breath and improving gingivitis through an efficacy test on a test product to which the oral composition of the present invention is applied.

As used herein, the term " antimicrobial effect "means an action of inhibiting the growth of bacteria and fungi by a drug or a physiologically active substance, and includes a broad concept including an effect of inhibiting, preventing, and killing the growth of microorganisms .

The backbone extract may be obtained by extracting various organs or parts of the backbone, that is, leaves, flowers, fruits, stems, roots, branches and bark, and preferably from the backbug.

The extract may contain all the extracts obtained by conventional extraction methods known in the art such as organic solvent extract, water extract extracted with water, supercritical extract, and the like. Preferably, the extract may be an extract obtained by extracting with water, an organic solvent, or a mixture thereof. Examples of the organic solvent include, but are not limited to, lower alcohols such as methanol, ethanol, isopropyl alcohol and butanol; Polyhydric alcohols such as glycerol, ethylene glycol, propylene glycol and 1,3-butylene glycol; And hydrocarbon solvents such as petroleum ether, methyl acetate, ethyl acetate, benzene, hexane, methylene chloride, diethyl ether, dichloromethane and chloroform, or a mixture thereof.

The nutmeg extract according to the present invention can be produced using an extraction method well known to those skilled in the art. That is, it is possible to prepare the pulverized beans by shredding and pulverizing, extracting the extracting solvent by adding 1 to 20 times the volume of the beans, and then selectively (decompressing) the concentrate, drying or refining. More specifically, an extraction solvent is added to extract an active ingredient substance as in the above-mentioned method, (a) heating at a temperature of 50 to 100 ° C for 1 to 20 hours in a state where a cooling condenser is installed to prevent evaporation of the solvent, (B) immersing the mixture at a temperature of 5 to 37 DEG C for 1 to 15 days to extract the active ingredient. That is, it can be produced by an extraction method such as stirring extraction, reflux cooling extraction, cold extraction, ultrasonic extraction or supercritical extraction.

The extract of L. thunb extract can be used as it is in a liquid state by extraction solvent or diluted in a solvent, or can be used in powder form. The pulverization may be carried out by a conventional method known in the art such as freeze-drying, spray drying, etc., and preferably a method of removing moisture by freeze-drying may be used.

The extract of the present invention includes not only the above-mentioned extraction solvent, but also an extract which has been subjected to a conventional purification process. Specifically, the extract of the present invention can be purified by an extraction solvent purification process, for example, a membrane separation using a filtration membrane having a cut-off value at a certain molecular weight, various chromatography for separation according to size, charge, hydrophobicity or affinity And a purification process such as separation by the above-mentioned method may be additionally carried out.

The composition for oral use according to the embodiments of the present invention comprises 0.01 to 1% by weight, more preferably 0.03 to 0.5% by weight, of the rosemary extract, based on the total weight of the composition.

If the content is less than 0.01% by weight, the antibacterial activity is not exhibited. If the content is more than 1% by weight, oral stimulation is not exhibited, or the stability of latex extract is poor.

According to the embodiments of the present invention, the oral composition of the present invention may contain the nutritional extract and the bicarbonate together to improve the antimicrobial activity of the nutritional extract, preferably sodium bicarbonate.

That is, the inventors of the present invention have found that when the extract and baker's extract are simultaneously used, the antimicrobial effect is significantly increased due to the synergistic effect, thereby preventing the bad breath and preventing and / or improving gingivitis.

The bicarbonate comprises 0.1 to 30% by weight, more preferably 0.5 to 20% by weight, based on the total weight of the oral composition. When the content is less than 0.5% by weight, the synergy effect of antibacterial activity is weak. When the content is more than 20% by weight, it is not preferable because the use feeling of taste and the like is poor.

The oral composition of the present invention may contain other ingredients such as a wetting agent, an abrasive, a pharmacological agent, a sweetener, a pH adjuster, a preservative, a binder, a flavoring agent, Foaming agent and the like.

The wetting agent may be used alone or in combination of a group consisting of concentrated glycerin, glycerin, sorbitol aqueous solution, amorphous sorbitol aqueous solution, polyethylene glycols and propylene glycol, and the amount thereof is 1 to 70% by weight based on the total weight of the oral care composition .

The abrasive may be one selected from the group consisting of precipitated silica, silica gel, zirconium silicate, calcium monohydrogenphosphate, anhydrous calcium hydrogenphosphate, hydrated alumina, hard calcium carbonate, heavy calcium carbonate, calcium pyrophosphate, insoluble metaphosphate and aluminum silicate Etc. may be used. The amount of the abrasive to be used is generally 1 to 60% by weight based on the total weight of the oral composition. The additives used in a small amount include additives commonly used in toothpaste such as sweeteners, pH adjusters, preservatives, colorants, and binders.

The active agent may be selected from the group consisting of sodium fluoride, sodium fluorophosphate, sodium fluoride, tin fluoride, chlorohexidine, allantoin chlorohydroxy aluminate, aminocaproic acid, zinc chloride, pyridoxine hydrochloride, tocopheryl acetate, Or more.

Examples of the binding agent include sodium carboxymethyl cellulose, carbohydrates, carrageenan, xanthan gum and alginates. Anionic and nonionic surfactants such as sodium alkylsulfate, sodium laurylsulfate, sucrose fatty acid esters and sorbitan fatty acid esters These may be used singly or in combination of two or more. The amount of such a binder to be used is generally 0.1 to 3% by weight, preferably 0.5 to 2% by weight, based on the total weight of the oral composition.

As the sweetening agent, saccharin sodium, xylitol, erythritol, aspartame and the like can be used. The amount of the sweetener to be used is generally 0.05 to 0.5% by weight based on the total weight of the oral composition.

As the pH adjusting agent, sodium phosphate, disodium phosphate, citric acid, triethanolamine and the like can be used.

As the preservative, benzoic acid, methylparaben, propylparaben, sodium benzoate and the like can be used. As the fragrance, peppermint oil, spearmint oil, menthol, or the like may be mixed and used. As other additives, enzymes such as texutaranase may be used.

Examples of such foaming agents include sodium lauryl sulfate, an anionic surfactant, a copolymer of polyoxyethylene polyoxypropylene (non-ionic surfactant), polyoxyethylene hardened castor oil, polyoxyethylene sorbitan fatty acid ester, etc. have. The amount of the foaming agent to be used is generally 0.5 to 5% by weight, preferably 0.5 to 3.5% by weight, based on the total weight of the oral composition.

Others include residual water.

Compositions for oral care may be used as formulations of toothpaste, mouthwash, mouthwash, mouthwash, viscous, chewing gum, ointment, patch or candy. It is also formulated by conventional means known to those of ordinary skill in the art to bring the active ingredient into contact with the oral cavity, such as a tooth, and to stick with any plaque or plaque that may be present.

The oral care composition may preferably comprise a composition for oral care comprising an abrasive, and most preferably a dentifrice composition.

The method for preparing the composition for oral administration of the present invention can be manufactured according to a method conventionally produced in the industry for producing dentifrices, mouthwashes and the like.

The composition for oral care containing the extract of Liliaceae and bicarbonate of the present invention can prevent the propagation of bacteria in the oral cavity.

The oral care composition of the present invention exhibits excellent efficacy for the removal and prevention of bad breath.

The present invention also provides a composition for oral care which is effective for the prevention and / or treatment of periodontal disease, particularly for preventing and / or treating gingivitis.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the embodiments according to the present invention can be modified into various other forms, and the scope of the present invention should not be construed as being limited to the above-described embodiments. The embodiments of the present invention are provided by way of example to facilitate a specific understanding of the present invention.

≪ Examples 1 to 8 and Comparative Examples 1 to 8 >

The dentifrice composition according to the present invention was prepared as shown in Table 1 below.

In order to prepare the dentifrice composition of these oral care compositions, a small amount of powdery components such as sodium carboxymethylcellulose, sodium saccharin and preservative are dispersed in an amorphous sorbitol solution which is a wetting component, diluted with purified water and then mixed in a primary mixer, And an abrasive such as needle-like silica was added and mixed in the secondary. Finally, a dentifrice composition was prepared by adding a foam stabilizer, a stabilizer, a perfume, and the like, followed by tertiary mixing.

Unit weight% Name Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 Example 8 Precipitated silica 20.0 20.0 20.0 20.0 20.0 20.0 20.0 20.0 Concentrated glycerin (98%) 30.0 30.0 30.0 30.0 30.0 30.0 30.0 30.0 Sorbitol aqueous solution (70%) 30.0 30.0 30.0 30.0 30.0 30.0 30.0 30.0 Sodium lauryl sulfate 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Carboxymethyl
Sodium cellulose 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Xanthan gum 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Sodium fluoride 0.22 0.22 0.22 0.22 0.22 0.22 0.22 0.22 Spices 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Sodium saccharin 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Citric acid, TEA Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Sodium hydrogencarbonate 10 20 10 20 Potassium hydrogencarbonate 10 20 10 20 Cetylpyridinium chloride (CPC) Acacia extract 0.03 0.03 0.5 0.5 0.03 0.03 0.5 0.5 Purified water To100 To100 To100 To100 To 100 To 100 To 100 To 100

Unit weight% Name Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 Comparative Example 7 Comparative Example 8 Precipitated silica 20.0 20.0 20.0 20.0 20.0 20.0 20.0 20.0 Concentrated glycerin (98%) 30.0 30.0 30.0 30.0 30.0 30.0 30.0 30.0 Sorbitol aqueous solution (70%) 30.0 30.0 30.0 30.0 30.0 30.0 30.0 30.0 Sodium lauryl sulfate 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Carboxymethyl
Sodium cellulose 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Xanthan gum 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Sodium fluoride 0.22 0.22 0.22 0.22 0.22 0.22 0.22 0.22 Spices 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Sodium saccharin 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Citric acid, TEA Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Sodium hydrogencarbonate 10 20 Potassium hydrogencarbonate 10 20 Cetylpyridinium chloride (CPC) 0.03 0.5 Acacia extract 0.03 0.5 Purified water To100 To100 To100 To100 To 100 To 100 To 100 To 100

Tooth compositions were prepared to perform the experimental examples according to the present invention using the ingredients according to Tables 1 and 2 above.

Test Example  1. Comparison test of antibacterial activity

2 ml of the test solution diluted from 1: 20 to 1: 5120 in Examples 1 to 8 and Comparative Examples 1 to 8 and 18 ml of blood agar medium (blood agar base + 5% final concentration blood) were placed in a 9 cm petri dish Lt; / RTI > Then, the test bacteria were inoculated and cultured in an anaerobic chamber at 37 DEG C for 96 hours. The maximum dilution ratio at which the test bacteria did not grow was measured by MIC and the results are shown in Table 3 (using Bactron I, anaerobic chamber).

The test organisms were Fusobacterium nucleatum (ATCC 25586) and Porphyromonas gingivalis (ATCC 33277) was used.

Test bacteria F. nucleatum P. gingivalis Example 1 350 540 Example 2 > 20 > 20 Example 3 120 80 Example 4 > 20 > 20 Example 5 300 250 Example 6 > 20 > 20 Example 7 100 100 Example 8 > 20 > 20 Comparative Example 1 4300 4200 Comparative Example 2 3460 3400 Comparative Example 3 4500 3900 Comparative Example 4 4000 3400 Comparative Example 5 4300 4300 Comparative Example 6 2500 2400 Comparative Example 7 2400 2100 Comparative Example 8 1900 1700

Test Example  2. Clinical trial on the inhibition of gingivitis in dentifrice composition

1) Subjects to be tested

Subjects were divided into 10 groups according to their gender, from 30 to 50 years of age. The subjects were divided into groups of 120 subjects. Clinical experiments on the therapeutic effect of gingival inflammation of toothpaste according to the present invention (prepared in Examples 1 to 8) and comparative toothpaste (prepared in Comparative Examples 1 to 8) were carried out as follows.

2) Experimental method

The subjects were divided into two groups: the experimental group and the comparative group. The subjects were instructed to use three times a day before sleeping after 2 hours after the meal, and the initial gingival index was scored by subjecting the teeth to a denture base. After 3 months and 6 months, oral examinations were performed to check gingival index. The gingival index was measured by inserting a periodontal probe into the gingival sulcus and burning around each tooth without applying any force. After 30 seconds, the gingival index was measured. And the results were obtained.

score Contents 0 point No bleeding state 1 point Normal bleeding state 2 points Hemorrhage 3 points Triangle Bleeding 4 points Gingival hemorrhage

The results of clinical tests for the treatment of gingival inflammation with the dentifrice composition and the comparative composition according to the present invention are as follows.

Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 Example 8 Early 1.02 1.03 1.09 1.02 1.03 1.06 1.05 1.05 1 week 1.17 1.15 1.15 1.09 1.08 1.09 1.10 1.09 1 month 1.22 1.21 1.20 1.20 1.12 1.20 1.18 1.20 3 months 1.45 1.39 1.51 1.44 1.33 1.42 1.39 1.36 6 months 1.42 1.40 1.34 1.22 1.41 1.48 1.45 1.43

Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 Comparative Example 7 Comparative Example 8 Early 1.03 1.01 1.07 1.05 1.07 1.11 1.06 1.03 1 week 1.24 1.29 1.34 1.32 1.22 1.23 1.27 1.22 1 month 2.11 2.00 2.14 2.12 2.34 2.21 2.00 1.99 3 months 2.69 2.66 2.76 2.67 3.21 3.18 3.10 3.09 6 months 3.10 3.00 3.03 3.01 3.34 3.21 3.25 3.19

3) Consideration

As shown in the above clinical test results, the dentifrice compositions of Comparative Examples 1 to 8 had no or minimal effect of inhibiting gingival inflammation, and the dentifrice compositions of Examples 1 to 8 had a therapeutic effect of gingival inflammation compared to the oral compositions of Comparative Examples 1 to 8 .

Test Example  3. Experiment of removing bad breath

Clinical trials were conducted on the effect of removing the bad breath using the oral compositions containing the compositions of the examples and comparative examples in Tables 1 and 2, and the results are shown in Table 7.

1) Experimental method

Fifty men and women without dental caries were selected and cross-over test was performed for the beverage. The commercially available garlic powder was dispersed in water, allowed to stand for 24 hours, and diluted so that the measured value of the halimeter was 700 ppb or more. The diluted solution was used as a source of bad breath. Gargle was sprinkled with 15 ml of garlic diluted solution for 30 seconds. After 1 minute, the degree of halitosis was measured with a halimeter, and the mixture was used for cleaning for 30 seconds to 1 minute using the compositions of Examples and Comparative Examples. After 1, 5, and 30 minutes after brushing, the degree of halitosis was measured with a halimeter, and the persistence of halitosis suppression was measured. The results are shown in Table 7 below.

2) Experimental results

As shown in the following Table 7, in the comparative example, persistence of halitosis suppression was shown for 5 minutes after brushing, but after 30 minutes, almost no halitosis was observed, indicating that the persistence of halitosis inhibition was insufficient there was. On the other hand, in the case of the example, it was confirmed that persistence of the bad breath suppressing effect continued to be high even after 30 minutes passed. As can be seen from the results of the above described bad breath removal experiments, the dentifrice compositions of Examples 1 to 8 were superior to the dentifrice compositions of Comparative Examples 1 to 8 in removing bad breath.

(unit %) Experimental group Elapsed time after brushing 1 minute 5 minutes 30 minutes Example 1 90.1% 77.3% 65.4% Example 2 93.2% 80.6% 72.9% Example 3 94.1% 81.1% 71.4% Example 4 96.3% 86.3% 82.2% Example 5 89.5% 80.3% 67.7% Example 6 92.1% 83.1% 72.5% Example 7 94. 2% 84.3% 74.8% Example 8 95. 7% 85.5% 76.2% Comparative Example 1 68.2% 41.7% 35.2% Comparative Example 2 69.9% 50.2% 35.6% Comparative Example 3 67.8% 43.1% 36.9% Comparative Example 4 65.3% 45.2% 38.1% Comparative Example 5 70.1% 43.2% 35.1% Comparative Example 6 73.8% 51.1% 38. 2% Comparative Example 7 75.4% 49.9% 41.9% Comparative Example 8 79.2% 58.8% 48.1%

According to the present invention, the oral composition containing sodium hydrogencarbonate and succinic acid extract according to the present invention has an antibacterial effect due to the synergy effect of sodium hydrogencarbonate and succulent extract, Or improving the oral health by effectively exhibiting the improvement effect.

Claims (8)

Extracts from Zucchini; And
Wherein the oral composition comprises any one selected from the group consisting of sodium hydrogencarbonate, potassium hydrogencarbonate, and mixtures thereof. delete delete [2] The oral composition for preventing gingivitis according to claim 1, wherein the extract is from 0.01 to 1% by weight based on the total weight of the composition for preventing gingivitis. The oral composition for prevention of gingivitis according to claim 1, wherein any one selected from the group consisting of sodium hydrogencarbonate, potassium hydrogencarbonate and mixtures thereof is contained in an amount of 0.1 to 30% by weight based on the total weight of the oral composition for the prevention of gingivitis . Extracts from Zucchini; And
Sodium hydrogen carbonate, potassium hydrogen carbonate, sodium bicarbonate, potassium hydrogen carbonate, and mixtures thereof. delete delete