KR101480690B1 - Oral composition containing natural agent - Google Patents

Abstract

The present invention relates to an oral composition containing pine needle extract and myrrh extract. More particularly, the present invention relates to a composition for oral cavity containing a pine needle extract and a myrrh extract excellent in antibacterial activity so as to effectively control germs in the mouth, ≪ / RTI > The present invention also relates to an oral composition further comprising a salt in said composition. The composition for oral use according to the present invention is effective for preventing gum disease by suppressing bacteria in the oral cavity by the synergy effect of pine needle leaf extract and myrrh extract and the convergence effect of salt on the gum, The freshness and freshness can be maintained for a long time.

Pine needle extract, myrrh extract, salt, bad breath removal, prevention of gum disease, oral composition

Description

[0001] The present invention relates to an oral composition containing natural agent,

The present invention relates to an oral composition containing pine needle extract and myrrh extract, and a composition for oral use which further contains salt in the composition. More particularly, the present invention relates to an oral composition comprising pine needle extract Myrrh extract, and salt which is effective for gum disease.

Generally, halitosis in the oral cavity is usually produced by hydrolysis of proteins and peptides containing sulfur components by Gram-negative bacteria in a basic environment. The sulfur compounds generated through this process are hydrogen sulfide, methyl mercaptan, dimethyl sulfide, and the like. It is related to the occurrence of smell that the environment in which Gram positive bacteria predominate is converted into an environment in which Gram negative and anaerobic bacteria predominate. Positional factors such as decreased saliva secretion or increased oral pH may affect this conversion process. If the bacteria membrane present in the interdental space can not be removed effectively and continuously, the gram-negative bacteria remaining therein may degrade the amino acid including sulfur, resulting in smell. Periodontitis also contributes to the development of halitosis, since the substrate produced during the inflammatory process promotes bacterial growth. In addition, the process of corruption and the accompanying odor are accelerated when the bacteria accumulate in the tongue. Progression of periodontal disease and deepening of the periodontal pouch contribute to the development of bad breath. Therefore, the management of periodontitis is an important part of managing posterior teeth.

Therefore, in order to maintain the bad breath removal effect for a long period of time, it is necessary to sterilize the bacteria in the mouth, which causes bad breath, to fundamentally prevent bad breath.

It is known that tooth decay and periodontal disease are caused by plaque that is formed by the formation of a thin film that sticks to the tooth surface of saliva and attaches oral microorganisms to the community to form a community. That is, the tooth decay is caused by the corrosion of the enamel which is generated by dissolving the hydroxyapatite, which is the main component of the teeth, by the organic acid produced by the anaerobic bacteria in the prage which metabolizes the saccharide, and the periodontal disease is caused by the Accumulation, resulting in increased bacterial activity and weakening of host defense capacity. Therefore, in order to alleviate periodontal disease, the activity of periodontal bacteria should be inhibited to relieve inflammation of the gums.

Oral products containing various bactericides such as cetylpyridinium chloride and chlorohexidine hydrochloride are known to sterilize such oral bacteria, but chlorhexidine hydrochloride has a disadvantage of causing tooth discoloration, and cetylpyridinium is a chloride Is a cationic compound and its effect on the oral environment is reduced by half due to its strong reactivity with anionic substances.

The effect of salt on the oral cavity is generally known to be effective in preventing or treating gum diseases such as dental diseases, especially dental caries, and dental caries, due to the convergence action on the gums when the salt is used in the oral cavity. A number of oral compositions containing salt for the purpose of prevention and treatment of such gum disease have been proposed.

Accordingly, it is an object of the present invention to provide an oral composition which effectively sterilizes germs in the mouth to exhibit excellent bad breath removing effect and is effective for gum disease.

In order to attain the above object, the present invention provides an oral composition containing pine needle extract and myrrh extract.

The present invention also provides an oral composition characterized by further comprising a salt in the composition.

The composition for oral use according to the present invention can provide excellent halitosis removal effect over a long term and can provide excellent gum disease prevention effect and tooth decay prevention effect in the oral cavity.

The oral composition of the present invention contains a pine needle extract and a myrrh extract as bactericidal and antibacterial components.

In addition, the composition for oral use of the present invention further contains salt as a component for preventing gum disease.

Hereinafter, the preparation of the oral composition of the present invention will be described.

The pine needle extract used in the present invention is as follows.

Pine trees are about 80-90 species in Pinus, and their terpenes are mainly used in industry. The major components of pine needles are terpenetine oil, Cineole, Salinigrin, Coniferin, P-Cymen, Densipimaric acid, Retene contains chlorophyll, protein, crude fat, phosphorus, iron, enzyme, minerals, fat soluble vitamin A and vitamin C. The major components of pine needles are different depending on the species and season . Turpentine oil is a volatile fragrance material, a group of unsaturated hydrocarbons having the chemical formula of C ₁₀ H ₁₆ , and the sterilization and antibacterial action against microorganisms is known.

Monoterpenes and Sesquiterpenes are the main components of pine needles extracted from steamed distillation from leaves and branches of pine trees. Among monoterpenes, α-Pinene and β-Pinene ), Carene, β-Myrcene, Limonene, and others.

In addition, the pine has been closely related to the real life of our nation. Pine needles are most widely used because they are easier to take and easier to prepare than other parts. In fact, pine leaves contain common nutrients, amino acids, fatty acids, vitamins, and minerals. Currently pine leaves are widely used as green juice, alcohol, sweet potato, moon, tea, confectionary and health drink.

These pine leaves have been used as folk remedies because they have been known to exert various medicinal effects in empirical medicine. In relation to oral diseases, it was said that the bad poultry was washed in water and chewed a few. In the stomatitis, it was said that the pine bark liquid was put on the skin and put on the affected part. In addition, toothpastes brush the pine needles with boiled water, and when the gums are poured, they put salt into the pine needle for a long time, and in the thrush, the pine needles are dipped in a handful of fingers to bite their juices in their mouths. It was said that brushing with water was effective.

The pine leaf extract used in the present invention may be those prepared by conventional methods known in the art. Preferably, 50% of the pine leaves obtained by cutting the collected pine leaves to a size of 1.0 to 2.0 cm is dissolved in 70% ethanol solution Water and ethanol) is added in a ratio of 500 ml, and the mixture is dipped in water at room temperature for 7 days to extract and filter. The filtrate is concentrated under reduced pressure to obtain a concentrate.

The myrrh extract used in the present invention is as follows.

Myrrh is also known as myrrha, also known as 药, to distinguish it from the flower manganese used in dyes. It is a pale yellow or dark brown lump material which is dissolved in alcohol and used as a myrrh tincture in the fragrance of mouth. Contains essential oils, resins, and gums. It has long been used as a preservative, and it is a widely known fact that it has been used extensively in making mummies. In the New Testament, the Gospel of Matthew (2:11), it is well known that the Magi was given to the baby Jesus with frankincense and gold.

These myrrhs are commercially available from Commphora molmol Engl. And rubbery resin from the scars of the bark area of the plant (Olive and Burseraceae) have been used for thrush, gingivitis, and mouthwashes due to side effects such as pain and nausea.

The myrrh extract used in the present invention may be one prepared by a conventional method known in the art, preferably 1 kg of Commele species of Oleo-gum-resin The water vapor was flowed at a rate of 0.5 L / min under a pressure of 1.5 kg / cm ² for 2 hours, and then the water vapor, which flowed out, was cooled by passing through a cooler. Then, the collected extract was separated from the water layer, . The edible ethane is obtained by mixing with 50% extract concentration.

The type of salt used in the present invention is not limited, but preferably used are sun salt, refined salt, baked salt and the like.

The oral composition of the present invention contains 0.001 to 2.0% by weight of the pine needle extract obtained by the above-mentioned method in the total weight of the composition and the myrrh extract in an amount of 0.001 to 2.0% by weight based on the total weight of the composition. Based on the total weight of the composition, in an amount of 0.001 to 10.0% by weight.

The oral composition of the present invention is excellent in antimicrobial activity and halitosis reduction effect for inhibiting bacteria in oral cavity by the synergy effect of pine leaf extract and myrrh extract, and provides a preventive effect of periodontal disease by convergence effect of salt on gums.

The composition for oral use of the present invention is not particularly limited in its formulation, and may specifically include a formulation such as a toothpaste, mouthwash or mouthwash.

The compounding ingredients of the oral composition for use in the present invention may be varied depending on the use and the kind of the composition, in addition to the above-described pine extract mall, myrrh extract and salt.

The composition for oral use according to the present invention may be appropriately selected depending on the type of toothpaste. For example, when the formulation is toothpaste, common dentifrice ingredients include abrasives, wetting agents, foaming agents, binders, sweeteners, pH adjusting agents, preservatives, A perfume, a brightener, a pigment, a solvent and the like.

Examples of the abrasive include calcium carbonate, precipitated silica, aluminum hydroxide, calcium monohydrogenphosphate and insoluble sodium metaphosphate. These abrasives may be used alone or in admixture of two or more in an amount of 1 to 60% by weight, preferably 10 To 50% by weight.

As the wetting agent, glycerin, sorbitol solution, polyethylene glycol, propylene glycol, etc. are used singly or in admixture of two or more, and 10 to 60% by weight, preferably 20 to 50% by weight, based on the total weight of the composition. Examples of the foaming agent include anionic and nonionic surfactants such as sodium lauryl sulfate, sodium lauryl sarcosinate, sucrose fatty acid ester, polyoxyethylene hydrogenated castor oil, and polyoxyethylene polyoxypropylene copolymer. 0.5 to 5% by weight, preferably 0.5 to 3% by weight, based on the total weight is used.

As the binder, 0.1 to 5% by weight, preferably 0.1 to 2% by weight, based on the total weight of the composition, of carboxymethylcellulose sodium, hydroxymethylcellulose, carrageenan, xanthan gum and sodium alginate are used alone or in combination of two or more thereof . As the sweetening agent, it is preferable to use 0.05 to 5% by weight based on the total weight of the composition of sodium saccharin, aspartame, stevioside, xylitol, licorice acid, etc., alone or in admixture of two or more.

Examples of the pH adjusting agent include sodium phosphate, disodium phosphate, trisodium phosphate, sodium pyrophosphate, citric acid, sodium citrate and tartaric acid. Examples of the preservative include methylparabenoxybenzoate, propylparabenoxybenzoate, sodium benzoate, Mix and use.

Examples of the active ingredient include sodium fluoride, sodium fluorophosphate, tin fluoride, chlorohexidine, allantoin chlorohydroxy aluminate, aminocaproic acid, tranexamic acid, triclosan, cetylpyridinium chloride, zinc chloride, pyridoxine hydrochloride, Tocopherol and the like may be used alone or in admixture of two or more. Examples of the perfume include a mixture of peppermint oil, spearmint oil, menthol, anethole, etc. in an appropriate amount, titanium oxide as a brightener, It is a solvent for ethanol and is prepared according to the usual toothpaste manufacturing method.

Hereinafter, the composition and action and effect of the oral composition according to the present invention will be described in more detail with reference to Examples and Comparative Examples. However, the present invention is not limited to these examples.

[Examples and Comparative Examples]

Toothpastes of Examples 1 to 2 and Comparative Examples 1 to 3 were prepared according to a conventional production method with the composition shown in Table 1 below.

ingredient Example 1 Example 2 Comparative Example 1 Comparative Example 2 Comparative Example 3 Sodium fluoride 0.30 0.30 0.30 0.30 0.30 Precipitated silica 17.00 17.00 17.00 17.00 17.00 Pine leaf extract 0.10 0.10 0.10 - Myrrh extract 0.10 0.10 - - 0.10 Purified salt 1.00 Concentrated glycerin 3.00 3.00 3.00 3.00 3.00 Sorbitol solution 10.00 10.00 10.00 10.00 10.00 Sodium carboxymethylcellulose 1.00 1.00 1.00 1.00 1.00 Sodium saccharin 0.10 0.10 0.10 0.10 0.10 Paraoxybenzoic acid methyl 0.20 0.20 0.20 0.20 0.20 Titanium oxide 0.10 0.10 0.10 0.10 0.10 Spices 1.00 1.00 1.00 1.00 1.00 Sodium lauryl sulfate 2.00 2.00 2.00 2.00 2.00 Purified water To 100 To 100 To 100 To 100 To 100

[Test Example 1] Comparison test of antibacterial activity

2 ml of the test solution diluted from 1: 20 to 1: 5120 in Example 1 and Comparative Examples 1 to 3 and 18 ml of the blood agar medium (blood agar base + 5% final concentration blood) were placed in a 9 cm petri dish Poured. After that, the test bacteria were inoculated and cultured in an anaerobic chamber at 37 ° C for 96 hours. The maximum dilution ratio (MID) for which the test bacteria did not grow was designated as MID (using Bactron I. anaerobic chamber), and the maximum dilution ratio (MID) for inhibiting bacterial growth is shown in Table 2 below.

<Test bacteria>

① Bad breath causative bacteria: Fusobacterium nucleatum ATCC 25586, Porphyromonas gingivalis ATCC 33277

② Cariogenic bacteria: Streptococcus mutans NCTC 10449, Actinomyces viscosus ATCC 15987

③ Main disease causing bacteria: Actionbacillus actinomycetemcomitans ATCC 29522

Bacterial growth inhibition Maximum dilution rate (MID) Test bacteria Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Fusobacterium nucleatum 2560 > 20 160 320 Porphyromonas gingivalis 2560 > 20 320 160 Streptococcus mutans 1280 > 20 320 640 Actinomyces viscosus 5120 20 640 1280 Actionbacillus
Actinomycetemcomitans 1280 > 20 160 160

As can be seen from the results of Table 2, the composition of Comparative Example 1, which contained no active ingredient, exhibited almost no antimicrobial effect because the inhibitory effect on oral pest was not observed. In Comparative Example 1 containing pine needle extract 2 and mulberry extract in Comparative Example 3 showed more effective antibacterial effect than Comparative Example 1. However, it can be seen that Example 1, in which pine needle extract and myrrh extract are used in combination, shows an excellent antibacterial effect against various oral pest bacteria as compared with Comparative Examples 2 and 3. It was confirmed that the combined use of pine needle extract and myrrh extract showed synergistic effect in increasing the antibacterial activity.

[Test Example 2] Measurement of the number of bacteria in saliva

40 ml of a 0.1% peptone solution (1000 ml of 0.9% NaCl and 1 g of peptone) was dispensed into each 15 ml Falcon tube in 5 ml aliquots, and then 40 peptone samples were subjected to a peptone solution After allowing to stand for 15 seconds, the peptone solution was diluted 10-fold with sterilized 0.067 M phosphate buffer solution (pH 7.2). 100 μl of the diluted solution was dispensed into Mitis Salivarius medium, plated on a flat plate with sterilized bending glass, and incubated in a 37 ° C incubator for 12 hours. Then, an anaerobic chamber At 37 &lt; [deg.] &Gt; C for 48 hours, and then cultured in each medium using a Colony counter Streptococcus The colony of mutans was measured and recorded. Streptococcus The colony of mutans was confirmed by microscopic examination and the strain Streptococcus mutans NTCC 10449 to check the form of the cultured colony in the time and under the same conditions as in the MSA, it was measured by selecting only the colonies of Streptococcus mutans in the test subjects. For each subject, diluted peptone juice was dispensed into two plates of Mitis Salivarius medium, and colonies were measured on the first and second occasions. The average value of the two measurements was taken as the number of Streptococcus mutans in the subjects. The measurement results are shown in Table 3 below.

Measurement of bacterial count in saliva Elapsed time Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Immediately after the experiment 109 108 110 112 after 2 weeks 21 98 65 54

As can be seen from the results in Table 3, the composition of Comparative Example 1, which contained no pine needle extract and myrrh extract, was Streptococcus the number of mutans was not substantially reduced, and in Comparative Example 2 containing pine needle extract or Comparative Example 3 containing myrrh extract, the values were lower than those of Comparative Example 1. However, it can be seen that Example 1, in which pine needle extract and myrrh extract are used together, shows excellent results as compared with Comparative Examples 2 and 3. From these results, it was confirmed that the combined use of pine needle extract and myrrh extract was effective in reducing the number of bacteria significantly due to the synergistic effect.

[Test Example 3] Measuring effect of removing bad breath

Eighty subjects were stopped for 48 hours after halting the oral environment management and then halitosis was measured. For each group, Examples 1 to 2 and Comparative Examples 1 to 3 were soaked with a test rinse. The degree of bad breath was measured again 30 minutes after brushing. Then, each test tooth solution was used twice a day for 28 days. 7 days, 14 days, 21 days and 28 days after the brushing, and the degree of bad breath was measured again at the time of 30 minutes after brushing, and the reduction effect of the bad breath according to the use period of the toothpaste composition is shown in Table 4 below.

Saliva score Elapsed time Example 1 Example 2 Comparative Example 1 Comparative Example 2 Comparative Example 3 Immediately after the experiment 220 220 219 218 221 7 days 170 150 222 205 208 14 days 145 130 217 196 195 21st 115 103 219 185 187 28th 102 96 217 174 176

The results of Table 4 show that the oral composition of Comparative Example 1, in which no active ingredient was used at all, showed almost no change in the odor score. On the other hand, the composition of Example 1 in which the pine needle extract and the myrrh extract were used in combination showed a significant decrease in the score of halitosis. In Comparative Example 2 and Comparative Example 3 in which the pine needle extract and myrrh extract were respectively used, a reduction effect was also exhibited, but the effect was not so large when compared with Example 1 used in combination. Furthermore, Example 2 using pine needle extract and myrrh extract together with salt could confirm that the effect was remarkably different.

[Test Example 4] Measurement of gum disease prevention effect

의 치은염 평점 기준에 따라 측정하였다.The gingival index was measured to confirm the gum disease prevention effect, and the method was as follows. First, after screening 60 subjects, they were not allowed to take oral medications that could affect the antibiotic dosing and experimentation during the experimental period. Twenty subjects were each divided into Comparative Example 1 and Examples 1 and 2, and the gingival index was changed by Talbott, Mandel and Chilton

Of gingivitis.

의 치은염 지수><Modified by Talbott, Mandel and Chilton

Gingivitis Index>

의 치은염평점기준

Gingivitis Rating Criteria

0: No inflammation

1: mild gingivitis (weak change of color tone and weak edema) - No bleeding in periodontal pouch

2: Middle gingivitis (redness, edema) - Hemorrhage in measuring periodontal pouch

3: High grade gingivitis (ulcer) - tendency to bleed spontaneously

Gingival index Comparative Example 1 Example 1 Example 2 Initial value 1.60 0.21 1.58 0.21 1.61 0.21 2 weeks 1.62 + - 0.25 1.32 ± 0.22 1.04 + - 0.14 4 weeks 1.59 ± 0.22 1.13 ± 0.18 0.72 ± 0.16 Improvement index 0.01 ± 0.23 0.45 ± 0.20 0.89 ± 0.18

The results of Table 5 show that Comparative Example 1, in which the pine needle extract, the myrrh extract and the salt were not blended, showed almost no gingival relaxation effect. In Example 1, in which the pine needle extract and the myrrh extract were used in combination, the gingival improvement effect was shown to some extent, but in the case of Example 2 in which the salt was added to the toothpaste, the gingival improvement effect was much better. This is because the concentration of salt on the gums causes the gum disease prevention effect to be further increased.

Claims (7)

Pine needle extract and myrrh extract. The oral composition of claim 1, wherein the composition further comprises salt. The oral composition according to claim 1, wherein the pine needle extract is contained in an amount of 0.001 to 2.0 wt% and the myrrh extract is contained in an amount of 0.001 to 2.0 wt% The oral composition according to claim 2, wherein the salt is contained in an amount of 0.001 to 10.0% by weight based on the total weight of the composition. The oral composition according to claim 1, wherein the composition has antibacterial activity. 3. The oral composition according to claim 1 or 2, wherein the composition is characterized by the removal of bad breath. The oral composition according to claim 1 or 2, wherein the composition alleviates gingivitis.