KR20240011429A - Oral care toothpaste composition containing herbal extract - Google Patents

Abstract

The present invention relates to an oral care toothpaste composition containing a herbal extract mixed with eucalyptus extract, mugwort extract, golden extract, ginseng extract, centella asiatica extract, and tea tree extract, wherein the composition has antibacterial properties against bacteria that cause cavities, periodontitis, or bad breath. It is highly effective and can be easily used as a toothpaste composition.

Description

Oral care toothpaste composition containing herbal extract {Oral care toothpaste composition containing herbal extract}

The present invention relates to an oral care toothpaste composition containing a herbal extract, and more specifically, to an oral care toothpaste composition containing a herbal extract mixed with eucalyptus extract, mugwort extract, goldenrod extract, ginseng extract, centella asiatica extract, and tea tree extract. It's about.

More than 400 types of bacteria grow in the human oral cavity, and the number of bacteria reaches 10 billion. In addition, the number of bacteria at the level of 10 ⁸ to 10 ⁹ CFU/ml has been detected in saliva. Teeth are perforated with many tiny holes, so bacteria that cause cavities enter these holes and cause disease in the teeth, which is cavities. These cavities are one of the most common diseases seen in teeth and are also called dental caries. It is an infectious bacterial disease in which food in the oral cavity is decayed by acid-resistant streptococci and lactic acid bacteria, and tooth components are destroyed by acid. In Korea, the prevalence of tooth decay in permanent teeth is approximately 80%, and it is reported that each person has an average of 2 to 3 cavities.

It has been revealed that the causes of cavities are a combination of many factors, but the most important one is plaque. Plaque contains a variety of bacteria, especially acid-resistant streptococci and lactic acid bacteria. Among them, Streptococcus mutans is known to be the main cavity-causing bacteria. As Streptococcus mutans attaches to the surface of the teeth and grows, it uses sugar in food to produce acid, which erodes the teeth and at the same time invades dental osteocytes, causing various types of alveolar bone erosion and gum disease. More specifically, the first stage in the development of cavities is the process in which plaque is formed on the tooth surface by cavity-causing bacteria, and the second stage is the process in which organic acids are produced within plaque, and the second stage is the process of organic acids and proteolytic enzymes produced within plaque. The third step is to demineralize the minerals that make up the tooth surface and further dissolve the organic substances. In addition, in the case of periodontal disease, plaque attached to the tooth surface and periodontal tissue is the main cause of the occurrence and progression of periodontal disease. Bacteria in the plaque produce harmful metabolites or bacterial enzymes, resulting in gingivitis. It is known to be progressing.

Bad breath refers to odor originating from the oral cavity and adjacent organs, and many people complain of inconvenience in their daily lives due to bad breath. 85-90% of bad breath originates from the mouth, especially the back of the tongue. The main components of bad breath are volatile sulfur compounds, and 90% of the total amount of volatile sulfur compounds are hydrogen sulfide made from cysteine, and methyl mercaptan and dimethyl sulfide made from methionine. These ingredients are mainly produced by protein enzymes secreted by anaerobic bacteria, and the back of the tongue is their most important habitat. This area is not cleaned well by saliva and has many small depressions, making it a place where bacteria continue to live. The production of volatile sulfur compounds by anaerobic bacteria is the most important cause of bad breath, but it can also be caused by other oral diseases such as cavities and periodontitis. Many types of anaerobic bacteria are involved in causing bad breath, but in particular, the representative bacteria causing bad breath are Porphyromonas gingivalis and Prevotella intermedia , which secrete many types and large amounts of enzymes. ), Fusobacterium nucleatum , Bacteoides forsythus , Treponema deticola , etc., are also known as representative causative bacteria that cause periodontitis. In order to suppress bad breath, it is most effective to suppress the growth of anaerobic bacteria that produce volatile sulfur compounds, the main component of bad breath. Conventionally, tongue scrapers are used to remove bacterial growth substrates from the tongue. To remove or inhibit the growth of anaerobic bacteria or the production of volatile sulfur compounds by anaerobic bacteria, metal salts such as zinc chloride or disinfectants such as alcohol or chlorhexidine were used. However, these metal salts and disinfectants suppress the development of not only anaerobic bacteria that cause bad breath, but also other microorganisms in the oral cavity, and since these substances are not swallowed through the esophagus, they do not reach the back of the tongue, which is an important place in the occurrence of bad breath, and are mainly stored in the front of the mouth. It is gargled and spit out of the mouth. Therefore, there is a problem that the back of the tongue, which is the main growth site of anaerobic bacteria that cause bad breath, cannot be disinfected. In addition, these substances are diluted by saliva and swallowed into the esophagus along with saliva, so the effect in the oral cavity is only about 20 minutes to 2 hours, and then microorganisms proliferate again in the oral cavity, causing bad breath. Therefore, metal salts or disinfectants used to reduce bad breath are only effective for a short period of time, and bad breath frequently recurs.

Recently, there have been attempts to suppress the growth of anaerobic bacteria using lactic acid bacteria (Korean Patent No. 10-1062779, etc.). However, when these lactic acid bacteria are administered orally, they are diluted by saliva like metal salts or disinfectants and enter the esophagus directly. It is difficult to swallow and remain in the oral cavity. In addition, because the acid component of lactic acid bacteria causes cavities, there is a problem that it is not good for oral hygiene when administered to the oral cavity for a long period of time.

Toothpaste is a quasi-drug used when brushing the surface of teeth, and is mainly used for whitening, sterilizing, and interdental cleaning effects. For this purpose, toothpaste generally contains ingredients such as abrasives, humectants, foaming agents, and sweeteners. It is done including. Recently, much research has been conducted to manufacture various functional toothpastes to improve the effects of suppressing cavities, periodontitis, and bad breath. Moreover, periodontal disease has recently been attracting attention as not only a chronic infection of gum tissue, but also as a risk factor for myocardial infarction, arteriosclerosis, diabetes, and premature birth. Therefore, it suggests that the use of toothpaste may serve as a foundation for improving various health conditions. Meanwhile, in general, 0.05 to 0.1 is added to toothpaste or mouthwash to prevent cavities or periodontitis. Fluoride at a w/v% concentration is often used. According to recent reports, the use of fluoride compounds in toothpaste or mouthwash can prevent dental caries, but long-term and repeated use of fluoride compounds promotes inflammation in the gums. It turns out that it does. Therefore, there is a need for a toothpaste composition that can suppress the phenomenon of sensitive teeth and prevent dental caries without using fluoride.

Eucalyptus is the fruit of Eleutherococcus sessiliflorus or Acanthopanax sessiliflorus , and the root bark or bark is called Eucalyptus or Eucalyptus for medicinal purposes and is known to have various physiological activities, but little research has been done on the fruit to date.

Meanwhile, while researching the various physiological activities of each ingredient that can be used as a toothpaste raw material, the present inventors confirmed that complex extracts of various herbal medicines have an inhibitory effect on various strains related to dental caries, periodontal disease, and bad breath, and thus developed the present invention. Completed.

Republic of Korea Registered Patent No. 10-1198634 (Title of invention: Natural edible antibacterial film composition for cavity prevention and manufacturing method thereof, Applicant: Korea National University of Transportation Industry-Academic Cooperation Foundation, Registration date: November 1, 2012) Republic of Korea Patent No. 10-1062779 (Title of the invention: Bifidobacterium adolesentis SPM1005 strain showing growth inhibitory activity of cavity-causing bacteria and the Bifidobacterium adolesentis SPM1005 strain or its culture as an active ingredient Food and pharmaceutical composition for preventing tooth decay, Applicant: Sahmyook University Industry-Academic Cooperation Foundation, Date of registration: August 31, 2011) Republic of Korea Patent Publication No. 10-2003-0093891 (Title of the invention: Propolis toothpaste composition and method for manufacturing the same, Applicant: Kim Hee-seong, Publication date: December 11, 2003) Republic of Korea Patent No. 10-1494436 (Title of the invention: New compound derived from quince fruit, Applicant: Republic of Korea, Registration date: February 11, 2015) Republic of Korea Patent Publication No. 10-2017-0054314 (Title of the invention: Composition for preventing and treating allergic or inflammatory diseases containing eucalyptus extract as an active ingredient, Applicant: Republic of Korea, Publication date: May 17, 2017)

The purpose of the present invention is to provide an oral care toothpaste composition containing herbal extracts.

The present invention relates to an oral care toothpaste composition containing a herbal extract, and more specifically, to an oral care toothpaste composition containing a herbal extract mixed with eucalyptus extract, mugwort extract, goldenrod extract, ginseng extract, centella asiatica extract, and tea tree extract. will be.

The herbal extract is 20-250 parts by weight of mugwort extract, 20-250 parts by weight of golden extract, 20-250 parts by weight of ginseng extract, 20-250 parts by weight of Centella asiatica extract, and 1400-2400 parts by weight of tea tree extract, based on 100 parts by weight of eucalyptus extract. It may contain additives.

The herbal extract may be contained in 0.2 to 2% by weight in the toothpaste composition.

The toothpaste composition may include silicon dioxide, allantoin chlorohydroxyaluminum, aminocaproic acid, concentrated glycerin, D-sorbitol solution, xanthan gum, lactoferrin, xylitol, aminocaproic acid, sodium cocoyl glutamate, mastic oil and purified water. .

At this time, the toothpaste composition contains 10 to 20% by weight of silicon dioxide, 0.01 to 0.1% by weight of allantoin chlorohydroxyaluminum, 0.02 to 0.2% by weight of aminocaproic acid, 1 to 5% by weight of concentrated glycerin, and 40 to 40% of D-sorbitol solution. 60% by weight, xanthan gum 0.5~1.2% by weight, lactoferrin 0.05~0.2% by weight, xylitol 0.01~0.1% by weight, aminocaproic acid 0.01~1% by weight, sodium cocoyl glutamate 0.5~3% by weight, mastic oil 0.05~ It may contain 0.5% by weight and 15-40% by weight of purified water.

Hereinafter, the present invention will be described in detail.

The present invention relates to a toothpaste composition containing a herbal extract mixed with eucalyptus extract, mugwort extract, goldenrod extract, ginseng extract, centella asiatica extract, and tea tree extract.

The toothpaste composition is characterized by an antibacterial effect against Streptococcus mutans strain, which is a cavity-causing bacteria.

The toothpaste composition contains bacteria that cause periodontitis or bad breath, such as Porphyromonas gingivalis , Prevotella intermedia , Fusobacterium nucleatum , and Bacteoides porcidus ( It is characterized by an antibacterial effect against one or more strains selected from the group consisting of Bacteoides forsythus ) and Treponema deticola .

The herbal extract is 20-250 parts by weight of mugwort extract, 20-250 parts by weight of golden extract, 20-250 parts by weight of ginseng extract, 20-250 parts by weight of Centella asiatica extract, and 1400-2400 parts by weight of tea tree extract, based on 100 parts by weight of eucalyptus extract. It may contain additional ingredients, but if the content range of each raw material exceeds this range, the ability to inhibit plaque or tartar formation, the antibacterial effect, or the bad breath inhibition effect may be reduced, which is not desirable.

0.1 to 5 parts by weight of L-lysine may be added to the toothpaste composition based on 100 parts by weight of eucalyptus extract, and the addition of L-lysine suppresses weakening of the gums.

The herbal extract may be contained in 0.2 to 2% by weight in the toothpaste composition.

Additionally, the toothpaste composition of the present invention may include silicon dioxide, allantoin chlorohydroxyaluminum, aminocaproic acid, concentrated glycerin, D-sorbitol solution, lactoferrin, xylitol, aminocaproic acid, sodium cocoyl glutamate, mastic oil, and purified water. , at this time, preferably 10 to 20% by weight of silicon dioxide, 0.01 to 0.1% by weight of allantoin chlorohydroxyaluminum, 0.02 to 0.2% by weight of aminocaproic acid, 1 to 5% by weight of concentrated glycerin, and 40 to 60% by weight of D-sorbitol solution. Weight%, lactoferrin 0.05~0.2% by weight, xylitol 0.01~0.1% by weight, aminocaproic acid 0.01~1% by weight, sodium cocoyl glutamate 0.5~3% by weight, mastic oil 0.05~0.5% by weight, and purified water 15~40% by weight. % may be included. At this time, if the content of each component is outside this range, the physical properties of the toothpaste composition may not be maintained well or the preservation effect of the toothpaste containing herbal extract may deteriorate. Additionally, the antibacterial and bad breath inhibition effects of the toothpaste composition may be reduced.

The eucalyptus extract can be extracted from eucalyptus, which is a seed of the Eucalyptus tree, using water, C1 to C4 alcohol, or a mixed solution thereof as a solvent, and the C1 to C4 alcohol is methanol, ethanol, propanol, isopropanol, butanol, and isobutanol. It can be selected from a group consisting of:

Water, C1 to C4 alcohol, or a mixed solution thereof used in the preparation of the extract can be used in an amount of 1 to 40 times the volume (1 to 40 liters per kg) based on the weight of plants or seeds used, and preferably 5 to 40 times the volume. can be used. Extraction conditions for the extract may be 1 minute to 48 hours at 20 to 100°C. The above process can be repeated 1 to 4 times. The manufacturing temperature of the extract may be 20 to 100°C, but is not limited thereto. The extraction time is not particularly limited, but is preferably extracted within 10 minutes to 2 days, and the extraction device may be a conventional extraction device, an ultrasonic grinding extractor, or a fractionator. Each extract prepared in this way can be dried with hot air, dried under reduced pressure, or freeze-dried to remove the solvent.

The solid content of the eucalyptus extract used in the present invention may be 1 to 90% by weight.

On the other hand, the eucalyptus extract that can be most preferably used in the present invention is concentrated in the dark by adding 5 to 20 times the volume (5 to 20 liters per 1 kg) of 30 to 90% (v/v) ethanol aqueous solution compared to the raw material sample. It is better to use one.

The present invention relates to a toothpaste composition containing a herbal extract mixed with tanase, goldenrod extract, ginseng extract, centella asiatica extract, and tea tree extract. The composition has excellent antibacterial effect against bacteria that cause cavities, periodontitis, or bad breath.

Hereinafter, preferred embodiments of the present invention will be described in detail. However, the present invention is not limited to the embodiments described herein and may be embodied in other forms. Rather, it is provided to ensure that the content introduced here is thorough and complete, and to sufficiently convey the spirit of the present invention to those skilled in the art.

<Example 1. Preparation of herbal extract>

Herbal extracts were prepared by mixing various extracts in the ingredient ratios shown in Table 1 below. For each extract, an extract obtained by adding 10 times the volume (10 liters per kg) of 70% (v/v) ethanol aqueous solution compared to the raw material sample was used. In Examples 1-7, L-lysine was added to prevent gum disease.

condition eucalyptus
Extract (g) Mugwort Extract (g) Gold
Extract (g) Ginseng
Extract (g) Centella asiatica
Extract (g) tea tree
Extract (g) L-Lysine (g) Example 1-1 100 100 100 100 100 2000 0 Example 1-2 100 20 180 20 180 2000 0 Example 1-3 100 180 20 180 20 2000 0 Example 1-4 100 20 180 20 180 2000 0 Examples 1-5 100 20 20 20 20 2320 0 Example 1-6 100 250 250 250 250 1400 0 Example 1-7 100 100 100 100 100 1999 One

<Example 2. Preparation of toothpaste composition>

Toothpaste was prepared by mixing the herbal extract and each component in the component ratio shown in Table 2 below.

condition Example 2-1 Example 2-2 Example 2-3 Example 2-4 Example 2-5 Herbal extract (g) 0.25
(Example 1-1) 0.25
(Example 1-3) 0.25
(Example 1-7) 0.2
(Example 1-7) 2
(Example 1-1) Silicon dioxide (g) 13.5 13.5 13.5 20 10 Allantoin Chlorohydroxyaluminum (g) 0.04 0.04 0.04 0.1 0.01 Aminocaproic acid (g) 0.05 0.05 0.05 0.02 0.2 Concentrated glycerin (g) 3 3 3 One 5 Sorbitol solution (g) 50 50 50 40 60 Xanthan gum (g) One One One 1.2 0.5 Lactoferrin (g) 0.1 0.1 0.1 0.05 0.2 Xylitol (g) 0.05 0.05 0.05 0.01 One Aminocaproic acid (g) 0.4 0.4 0.4 0.01 One Sodium Cocoyl Glutamate (g) 1.2 1.2 1.2 3 0.5 Mastic oil (g) 0.3 0.3 0.3 0.05 0.5 Purified water (g) 30.11 30.11 30.11 34.36 19.09

<Comparative Example 1. Preparation of comparative composition>

A comparative composition was prepared by mixing each extract in the ingredient ratio shown in Table 3 below.

condition eucalyptus
Extract (g) Mugwort
Extract (g) Gold
Extract (g) Ginseng
Extract (g) Centella asiatica
Extract (g) tea tree
Extract (g) Comparative Example 1-1 0 200 0 200 100 2000 Comparative Example 1-2 0 300 400 500 600 700 Comparative Example 1-3 1000 1000 0 0 500 0 Comparative Example 1-4 100 5 5 5 5 2380 Comparative Example 1-5 2500 0 0 0 0 0 Comparative Example 1-6 100 400 400 400 400 800

<Comparative Example 2. Preparation of comparative toothpaste composition>

Toothpaste was prepared by mixing the herbal extract and each component in the component ratio shown in Table 4 below.

condition Comparative Example 2-1 Comparative Example 2-2 Herbal extract (g) 0 3.75
(Example 1-1 Herbal Medicine Extract) Silicon dioxide (g) 15.71 25 Allantoin Chlorohydroxyaluminum (g) 0.1 0.04 Aminocaproic acid (g) 0.12 0.05 Concentrated glycerin (g) 5 3 Sorbitol solution (g) 62 35 Xanthan gum (g) 0.8 One Lactoferrin (g) 0.1 0.1 Xylitol (g) 0.05 0.05 Aminocaproic acid (g) 0.01 0.4 Sodium Cocoyl Glutamate (g) 1.3 1.2 Mastic oil (g) 0.3 0.3 Purified water (g) 14.51 30.11

<Experimental Example 1. Confirmation of antibacterial effect>

Streptococcus mutans, Porphyromonas gingivalis, and Prevotella intermedia were cultured on TSA (trypticase soy agar) medium supplemented with hemin menadione, and then the minimum growth inhibitory concentration (MIC) was measured. and this is shown in Table 5. At this time, the herbal extract was diluted so that the solid content was 0 to 1000 ㎍/㎖, and each strain (bacteria) was used by adjusting the concentration to OD.600 = 0.2. In a 96-well plate (Falcon, USA), 100 ml of TSB (trypticase soy broth) supplemented with hemin menadione for bacterial culture used in the present invention, 50 ml of herbal extract dilution solution, and 50 ml of strain dilution solution were placed in a 96-well plate (Falcon, USA). After sequential addition, the mixture was incubated at 37°C for 24 hours while measuring the absorbance value using a UV-spectrophotometer every 4 hours. At this time, the minimum concentration at which turbidity of the strain was not detected on the bacterial growth curve was set as the minimum growth inhibition concentration.

condition MIC for Streptococcus mutans (μg/ml) MIC for Porphyromonas gingivalis (μg/ml) MIC for Prevotella intermedia (μg/ml) Example 1-1 4.7 5.2 1.2 Example 1-2 9.2 10.2 11.3 Example 1-3 2.2 2.1 2.6 Example 1-4 12.3 6.4 11.5 Examples 1-5 8.4 5.1 10.2 Example 1-6 7.4 4.2 8.3 Example 1-7 5.4 9.6 7.3 Comparative Example 1-1 43.2 42.6 46.2 Comparative Example 1-2 40.2 47.2 55.3 Comparative Example 1-3 55.1 44.3 51.2 Comparative Example 1-4 52.0 59.2 52.5 Comparative Example 1-5 67.3 60.3 58.3 Comparative Example 1-6 87.5 89.2 74.3

Referring to the results in Table 5, it can be seen that the antibacterial effect on oral bacteria of the compositions of Examples 1-1 to 1-7 of the present invention is significantly superior to that of the compositions of Comparative Examples 1-1 to 1-6. You can. These microbial replacement results were similar in replacement experiments with Fusobacterium nucleatum, Bacteoides forcidus, and Treponema deticora.

<Experimental Example 2. Sensory evaluation according to the use of toothpaste>

The toothpaste of Example 2 and Comparative Example 2 of the present invention was distributed to 50 adult men and women, and after use for 2 months, the effect of suppressing bad breath and gum inflammation was compared with the results of using a commercially available general toothpaste, and the result was 5 points. It was expressed using the RBI method (1 point: very bad, 2 points: bad, 3 points: average, 4 points: good, 5 points: very good).

condition Bad breath suppression effect Gum inflammation suppression effect Example 2-1 4.1 4.0 Example 2-2 4.3 4.2 Example 2-3 4.6 4.7 Example 2-4 4.1 4.3 Example 2-5 4.0 4.0 Comparative Example 2-1 3.1 3.1 Comparative Example 2-2 3.4 3.5

Referring to the results in Table 6, it is confirmed that people using the toothpaste of Example 2 evaluated the effect of suppressing bad breath and gum inflammation to be better than those using the toothpaste of Comparative Example 2. In addition, it can be seen that among the toothpastes, the toothpaste of Example 2-3 has the highest effect. In the case of the toothpaste of Comparative Example 2-2, although it contained herbal extracts, the effects of suppressing bad breath and suppressing gum inflammation were judged to be reduced due to the characteristics of the formulation according to the mixing conditions with toothpaste excipients.

Claims (5)

A toothpaste composition characterized by containing a herbal extract mixed with eucalyptus extract, mugwort extract, golden extract, ginseng extract, centella asiatica extract, and tea tree extract. According to paragraph 1,
The toothpaste composition includes silicon dioxide, allantoin chlorohydroxyaluminum, aminocaproic acid, concentrated glycerin, D-sorbitol solution, xanthan gum, lactoferrin, xylitol, aminocaproic acid, sodium cocoyl glutamate, mastic oil, and purified water. Characterized toothpaste composition. According to paragraph 1,
A toothpaste composition characterized in that L-lysine is added to the herbal extract. According to paragraph 1,
The toothpaste composition is characterized in that it has an antibacterial effect against Streptococcus mutans strain, which is a cavity-causing bacteria. According to paragraph 1,
The toothpaste composition is one selected from the group consisting of Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum, Bacteoides forsidus, and Treponema deticora, which are bacteria causing periodontitis or bad breath. A toothpaste composition characterized by an antibacterial effect against more than one type of strain.