KR102575768B1 - Natural Edible Film Compositions for Preventing or Improving Oral Diseases and Uses Thereof - Google Patents

Abstract

The present invention relates to a natural edible antibacterial film composition for preventing or improving halitosis, tooth decay or periodontal disease and its use, comprising a mixture of bee venom and honey as active ingredients; And it relates to a natural edible antibacterial film composition for preventing or improving halitosis, tooth decay or periodontal disease, including a film forming agent.
According to the edible antibacterial film composition made of natural antibacterial substances according to the present invention, there is no side effect on the human body, and the purified bee venom and honey contained in the film are effective in removing odor-causing substances and have antibacterial action against oral disease-causing bacteria. As a result, it can be used as a new concept of oral hygiene maintenance and tooth decay prevention product that can be easily carried and used by consumers by suppressing oral disease and removing bad breath for a long time, and can be used as an antibacterial film for food packaging. .

Description

Natural Edible Antibacterial Film Compositions for Preventing or Improving Oral Diseases and Uses Thereof

The present invention relates to a natural edible antibacterial film composition for preventing or improving oral diseases, preferably halitosis, tooth decay or periodontal disease, and its use.

As modern society becomes industrialized and westernized, the causes and symptoms of oral-related diseases are diversified due to changes in lifestyle and dietary habits, and are reported as serious social problems. Oral diseases such as dental caries (cavities), periodontal disease, halitosis and oral pain are recognized by WHO as one of the major health problems to be addressed in the future. According to the WHO report on dental disease, 60-90% of adolescents and 60% of adults worldwide suffer from dental caries and periodontal disease, and 15-20% of middle-aged adults have severe periodontal disease. These oral diseases are reported to be not only a major cause of tooth loss, but also a potential risk factor for diseases such as cardiovascular diseases, bacterial pneumonia, low birth weight infants, complications of diabetes, and osteoporosis.

In particular, the issue of bad breath, which is an important interpersonal obstacle in social life, has recently emerged, and accordingly, interest in oral health is increasing.

Bad breath is sometimes caused by acquired diseases, but in general, proteins and food waste in saliva are decomposed by microorganisms in the oral cavity and amino acids are decomposed by demineralization enzymes or amino enzymes to produce substances that cause odor. occurs by creating In addition, when garlic or red pepper is ingested, bad breath also occurs due to sulfides contained in these substances.

Representative components that cause such halitosis include volatile sulfide compounds (VSC). These volatile sulfides include hydrogen sulfide (H ₂ S), methyl mercaptan (CH ₃ SH), dimethyl There are mercaptans (Dimethyl mercaptan) and the like, and methyl mercaptan in particular is known as the main component of the disgusting smell among bad breath. In addition, volatile amine compounds, such as trimethyl amine or δ-aminovaleric acid, are also known as major components of odor components. components such as aldehydes, fatty acids, ammonia or pyridine.

Causes of bad breath can be largely classified into oral causes, extraoral causes, and psychogenic causes. , increase in salivary consistency, decrease in hydrogen ion concentration buffering capacity, increase in the amount and activity of oral microorganisms, and oral diseases such as tongue plaque attached to the back of the tongue and untreated dental caries and periodontal disease.

On the other hand, as described above, as a method of inhibiting the growth of oral pathogens that cause dental caries, periodontal disease, and bad breath, which are one of the main causes of bad breath, various types of antibacterial agents including antibiotics having bactericidal and bacteriostatic action against these bacteria was developed as an inhibitor and treatment for tooth decay, periodontal disease, pulp and apical infection.

However, oral disease treatment such as antibiotics as described above has a problem that can be used only as a treatment because long-term use is difficult because it can cause the appearance of resistant bacteria in the oral cavity and mycosis, along with side effects on the human body as a whole.

On the other hand, for the purpose of removing bad breath caused by the growth of bacteria in the mouth or spoilage of food and maintaining oral cleanliness, a liquid-type mouthwash has been developed that simply rinses and cleans the mouth. The liquid-type mouthwash has a weight of its own. It is large and bulky, and in most cases, a container such as an additional cup is required, so it is inconvenient to carry, and bad breath can be removed by rinsing the mouth. There was such an inconvenience as having to spit it out.

Therefore, it is convenient to use, can be used anytime, anywhere, effectively removes the smell in the mouth that occurs after eating or on an empty stomach, and is safe for the human body without worrying about side effects. am.

Recently, studies using edible films based on biopolymers such as proteins, carbohydrates, and fats have been actively conducted. This film is easily degraded compared to non-edible, non-degradable plastic polymer film, and ingredients such as flavors, colors, and sweeteners can be added to the edible film to give organoleptic characteristics to the contents, and to be edible including deficient nutrients. It can be used as a film, can be used as a food packaging material, such as being used as a material transfer barrier.

Under these circumstances, the inventors of the present invention, by ingesting and using the antibacterial and anti-caries edible film when necessary, the edible film is dissolved and the medicinal substance is released for a long time, so that it can be effectively used for removing bad breath without side effects, and furthermore, oral Research efforts were made to develop a bad breath remover that could improve the disease. As a result, the inventors of the present invention prepared a halitosis remover in the form of a film that can be easily dissolved in the mouth and can stay for a long time using purified bee venom and honey, which are natural substances harmless to the human body. Not only can it be consumed in a reasonable way, it can effectively remove bad breath to maintain oral cleanliness, and it can also prevent tooth decay caused by bacteria that cause tooth decay in the oral cavity. The present invention was completed by clarifying that it can be suppressed.

Accordingly, one object of the present invention is to provide a natural edible antibacterial film composition for preventing or improving halitosis, tooth decay or periodontal disease.

Another object of the present invention is to provide a food composition for preventing or improving bad breath, tooth decay or periodontal disease.

Another object of the present invention is to provide a quasi-drug composition for preventing or improving halitosis, tooth decay or periodontal disease.

Another object of the present invention is to provide a method for preparing a natural edible antibacterial film composition for preventing or improving halitosis, tooth decay or periodontal disease.

Other objects and advantages of the present invention will become more apparent from the following detailed description of the invention, claims and drawings.

The terms used herein are used for descriptive purposes only and should not be construed as limiting. Singular expressions include plural expressions unless the context clearly dictates otherwise. In this specification, terms such as "include" or "have" are intended to designate that there is a feature, number, step, operation, component, part, or combination thereof described in the specification, but one or more other features It should be understood that the presence or addition of numbers, steps, operations, components, parts, or combinations thereof is not precluded.

Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by a person of ordinary skill in the art to which the embodiment belongs. Terms such as those defined in commonly used dictionaries should be interpreted as having a meaning consistent with the meaning in the context of the related art, and unless explicitly defined in the present application, they should not be interpreted in an ideal or excessively formal meaning. don't

Hereinafter, the present invention will be described in more detail.

According to one aspect of the present invention, the present invention provides a mixture of bee venom and honey as an active ingredient; And it provides a natural edible antibacterial film composition for preventing or improving tooth decay, halitosis or periodontal disease, including a film forming agent.

In the present invention, "oral disease" refers to various diseases occurring in the oral region, and the oral region refers to the space in the mouth connected to the pharynx from the front lip to the back oral cavity. In the present invention, the oral disease is a concept that includes all diseases that occur in the oral cavity regardless of the disease, and non-limiting examples of the oral disease include dental caries (cavities), periodontal disease (periodontitis or gingivitis), bad breath, toothache , Shirin, etc.

The term of the present invention, "cavity" means worm-eaten teeth, and is a condition in which the hard tissues of the teeth are eroded and lost, and is also called caries. Tooth decay is an infectious bacterial disease in which a part of the hard calcified tissue of the tooth is dissolved and destroyed, causing pain and eventually causing tooth loss, and is the most common periodontal disease.

The term of the present invention, "periodontal disease" is often referred to as periodontal disease, and is divided into gingivitis and periodontitis according to the severity of the disease. Gingivitis is a relatively mild and reversible oral disease limited to the gums, that is, soft tissues, and periodontitis is when this inflammation progresses to the gingiva and around the alveolar bone.

There is a V-shaped gap between the gingiva (gum) and the tooth, and periodontal disease is when bacteria attack the lower gingival portion of this gingival sulcus, damaging the periodontal ligament and adjacent tissues. As inflammation progresses and more tissues are damaged, the groove develops into a periodontal pocket, and as periodontitis is severe, the depth of the periodontal pocket deepens. As the periodontal pocket deepens, the periodontal ligament becomes inflamed, and osteoclast differentiation is promoted by lipopolysaccharide (LPS) secreted by Porphyromonas gingivalis, resulting in alveolar bone loss.

In the present invention, the term "halitosis" is a smell derived from the oral cavity and adjacent organs, and 85 to 90% of bad breath originates from the oral cavity, particularly from the back of the tongue. The main component of bad breath is volatile sulfur compounds, 90% of which are hydrogen sulfide made from cysteine and methyl mercaptan and dimethyl sulfide made from methionine. These components are mainly produced by protein enzymes secreted by anaerobic bacteria, and the back of the tongue becomes the most important habitat. This area is not well cleaned by saliva and has many small depressions, making it a place where bacteria live continuously. The production of volatile sulfur compounds by anaerobic bacteria is the most important cause of bad breath, but other oral diseases such as dental caries, periodontitis, and dry mouth are also caused. Many types of anaerobic bacteria are involved in generating bad breath, and a non-limiting example of bad breath causing bacteria is Porphyromonas gingivalis , which secretes many types and large amounts of enzymes.

In order to achieve the above object, an edible film composition comprising a film forming agent, a solvent, a vitamin, a thickener, a wetting agent and an emulsifier in addition to a mixture of purified bee venom and honey, which are the active ingredients, is preferably composed of the bee venom and honey. The mixture may contain 1 to 10 μg of bee venom per 10 mg of the honey; The mixture of the purified bee venom and honey is mixed at 0.001 to 50% by weight of the total film composition to provide a natural edible antibacterial film composition for preventing or improving halitosis, tooth decay or periodontal disease, which disintegrates in the oral cavity.

That is, in the present specification, "natural edible antibacterial film" means "oral disintegrating film", and follows the meaning as known in the art, specifically dissolving, dispersing, etc. in the oral cavity. It refers to a film dosage form that can be disintegrated and taken. Such a film formulation is placed on the tongue and dissolved, but can be ingested by attaching to the roof of the mouth, sublingually, or the inside of the mouth (buccal).

In the present specification, "natural edible antibacterial film composition" means a mixture form containing components for preparing an oral disintegrating film, and includes without limitation a mixture form before a drying process for preparing an oral disintegrating film. .

The purified bee venom and honey of the present invention, as active ingredients of the present composition, can remove methyl mercaptan, the main component of bad breath, and have excellent antibacterial activity against oral disease-causing pathogens existing in the oral cavity, thereby eliminating bad breath as well as cavities and periodontal There is an effect of preventing or treating oral diseases such as diseases.

In one embodiment of the present invention, purified bee venom is obtained by obtaining bee venom from worker bees of Western species ( Apis mellifera ) and then purified by a simple purification method to use a melittin content of 63 ± 3%. As long as it can achieve, it is not limited thereto.

In one embodiment of the present invention, honey was used as acacia honey, but it is not limited thereto as long as it can achieve the object of the present invention.

The film-forming agent is a polymer capable of forming an orally disintegrating film, and any film-forming agent may be used as long as it can achieve the object of the present invention. In the present invention, the film forming agent includes starch, water-soluble starch, modified starch, polyvinylpyrrolidone, polyvinyl alcohol, pullulan, gelatin, guar gum, xanthan gum, polyethylene glycol, gum acacia, gum arabic, polyacrylic acid, amyl 0.5 to 35% by weight, preferably 5 to 25% by weight of one selected from the group consisting of rose, pectin, dextrin, chitin, geratin, gluten, casein and carrageenan alone or in combination of two or more. It may be used by adding, but is not limited thereto, and in one embodiment of the present invention, 1 to 20% by weight is added.

The solvent is used to dissolve or disperse the compositions forming the oral disintegrating film, and any solvent harmless to the human body may be used as long as the object of the present invention can be achieved. In the present invention, water may be used as the solvent, and purified water or distilled water may be used as the water, but is not limited thereto, but in one embodiment of the present invention, purified water is added.

Any humectant can be used as long as the object of the present invention can be achieved. As the wetting agent, any one or more materials selected from the group consisting of amorphous sorbitol, polyethylene glycols, propylene glycol, glycerin and concentrated glycerin may be used, but is not limited thereto, and in one embodiment of the present invention, 0.001 to 0.02 weight % is added.

Any emulsifier can be used as long as the object of the present invention can be achieved. As the emulsifier, emulsifiers commonly used in food may be used. Hydroxypropyl methyl Cellulose, Lecithin, Sodium Caseinate, Casein, Ammonium Alginate, Tama At least one material selected from the group consisting of Lind gum, polysorbate 80, sucrose fatty acid esters and glycerin fatty acid esters may be used, but is not limited thereto, and in one embodiment of the present invention, 0.001 to 0.02% by weight is added. .

In addition, the natural edible antibacterial film composition of the present invention, within the limit that does not deviate from the object of the present invention, from the group consisting of pH adjusting agents, nutrients, lubricants, flavoring agents, suspending agents, preservatives and surfactants known in the art One or more selected types may be further added.

In one embodiment, the present invention provides an emulsion slurry containing a mixture of an emulsifier, purified bee venom, and honey to an edible film composition solution prepared by mixing a film forming agent, a vitamin, a thickener, a wetting agent, and the remaining amount of a solvent to form a 60 The natural edible antibacterial film composition of the present invention is prepared by mixing in a water bath at ° C. The film-forming composition is prepared into a film by spreading and pouring the prepared solution on a tray and then drying it with hot air.

The composition for preventing or improving halitosis, tooth decay or periodontal disease of the present invention has excellent methyl mercaptan removal ability as a result of an experiment on removing methyl mercaptan, the main component of bad breath, to determine the effect of removing bad breath using purified bee venom and honey as useful ingredients. proved.

In addition, Streptococcus mutans remaining in the oral cavity ( Streptococcus mutans ), Streptococcus sanguis ( Streptococcus sanguis ), Porphyromonas gingivalis ( Porphyromonas Gingivalis ) and Fusobacterium nucleatum ( Fusobacterium nucleatum ) The removal ability test As a result, it was confirmed that the antibacterial effect was excellent.

At this time, if the content of the mixture of purified bee venom and honey, which are active ingredients, is more than 50% by weight based on the composition, if it is included excessively, the sensory function may decrease and cytotoxicity may occur, and if it is included too little, less than 0.001% by weight, the antibacterial activity is low and the efficacy is low. Since this may be halved, it is preferable to add about 20% of the active ingredient by weight.

The composition of the present invention can be prepared as a normal food, considering that the mixture of bee venom and honey, which are active ingredients, is a food material.

In the food composition of the oral disintegrating film dosage form of the present invention, the mixture of bee venom and honey is used in any amount as long as it sufficiently exhibits its intended functionality as a food and does not affect the suitability as a film dosage form in terms of physical properties, disintegration properties, etc. can be included as

The composition of the present invention may be a quasi-drug composition for preventing, improving or removing halitosis, tooth decay or periodontal disease.

When the mixture of bee venom and honey according to the present invention is used as a quasi-drug additive, the active ingredient of the mixture having a bad breath removal activity can be added as it is or used together with other quasi-drugs or quasi-drug ingredients, and can be appropriately used according to a conventional method. . The mixing amount of the active ingredient can be suitably determined depending on the purpose of use.

The quasi-drug composition for removing bad breath of the present invention is not particularly limited in its dosage form, and may be formulated in various forms in the form of quasi-drugs known in the art for removing bad breath.

In addition, in each formulation, the quasi-drug composition for preventing, improving or removing bad breath may be combined with other ingredients selected arbitrarily according to the formulation or purpose of use of other quasi-drugs. For example, conventional adjuvants such as thickeners, stabilizers, solubilizers, vitamins, pigments and flavors, and carriers may be included. The quasi-drug composition containing the mixture of bee venom and honey as an active ingredient of the present invention is derived from nature itself and has little toxicity and side effects, so it can be safely used as a quasi-drug material.

The quasi-drug composition for oral use of the present invention can be prepared in any formulation commonly prepared in the art, for example, toothpaste, mouthwash, mouthwash, gum, candy, oral spray, oral ointment, and oral varnish. , oral rinse and gum massage cream, but may have formulations such as, but are not limited thereto.

According to another aspect of the present invention, the present invention provides a method for preparing a natural edible antibacterial film composition for preventing or improving halitosis, tooth decay or periodontal disease, comprising the following steps:

(a) preparing an edible film composition solution by dissolving a film forming agent in a solvent, adding vitamins, a thickener and a wetting agent, and stirring the mixture;

(b) preparing an emulsion slurry by mixing an emulsifier with a mixture of bee venom and honey; and

(c) mixing the edible film composition solution of step (a) and the emulsion slurry of step (b) at 60°C; and

(d) casting the mixture of (c), molding it into a film form, and drying it with hot air.

Oral disintegrating film for preventing or improving halitosis, tooth decay or periodontal disease by applying the mixture in step (d) on a substrate and then drying it to the extent that the desired effect can be achieved using a drying means It can be prepared, at this time, the oral disintegrating film may be formed to a thickness of 1 to 200㎛.

Since the method of the present invention uses a composition, redundant details are omitted to avoid excessive complexity of the present specification.

According to the edible antibacterial film composition made of natural antibacterial substances according to the present invention, there is no side effect on the human body, and the purified bee venom and honey contained in the film are effective in removing odor-causing substances and have antibacterial action against oral disease-causing bacteria. It can be used as a new concept of oral hygiene maintenance and tooth decay prevention product that can be easily carried and used by consumers by suppressing the occurrence of oral diseases and removing bad breath for a long time, and can be used as an antibacterial film for food packaging. .

Figure 1 shows the evaluation results of cell viability for purified bee venom and honey in human lower gingiva epithelial cell.

Hereinafter, the present invention will be described in more detail through examples. These examples are intended to explain the present invention in more detail, and the scope of the present invention is not limited to these examples.

Example

Example 1. Evaluation of antibacterial activity of purified bee venom and honey against oral disease-causing bacteria

The present inventors measured the antibacterial activity of purified bee venom and honey against oral disease-causing bacteria as follows.

Purified bee venom was obtained from the venom sac of worker bees of Western species ( Apis mellifera L.), and then purified by a simple purification method, and the melittin content was 63 ± 3%, and was used in this example.

Honey can be honey of various wheat sources, but acacia honey was used in this example.

Target strains include Streptococcus mutans (ATCC 27175), a bacterium that causes tooth decay, Streptococcus sanguis (ATCC 10556), a bacterium that causes gum inflammation, and Porphyromonas gingivalis , a bacterium that causes periodontal disease. ) and Fusobacterium nucleatum, an anaerobic oral bacterium causing halitosis, were purchased from the Korea Microorganism Conservation Center (Daejeon, Korea).

S. mutans strain was cultured in aerobic conditions at 37° C. using BHI (Brain heart infusion broth, BD, France) medium; The S. sanguis strain was cultured under aerobic conditions at 37° C. using TSB (Trypticase soy broth, BD, France) medium supplemented with 5% sheep blood (MB cell, USA); P. gingivalis and F. nucleatum strains were cultured at 37°C in a TSA medium CO ₂ incubator supplemented with 10% sheep blood and 0.1% hemin (Sigma, USA).

In order to confirm the antibacterial activity effect on the oral disease causative bacteria, a plate medium diffusion method was used.

Briefly, S. mutans was smeared on a plate medium, and a paper disk (Advantec, Japan) with a diameter of 8 mm in which purified bee venom and honey, combined in various composition ratios, was accumulated was placed on the plate medium, and cultured for 48 hours. The size of the resulting transparent hypoallergenic ring was measured.

As a result, as shown in Table 1 below, the higher the purified bee venom content, the higher the antibacterial efficacy, but the sensory evaluation was disadvantageous in the group exceeding 10 μg, that is, in the group treated with 100 μg, because the bitter taste became stronger.

sample number Purified bee venom (μg) honey (mg) inhibition zone (mm) One 0.1 10 12.7 2 One 10 18.9 3 10 10 > 20 4 100 10 > 30

In addition, in order to confirm the cytotoxicity according to the composition ratio of purified bee venom and honey, the inventors tested the purified bee venom and honey of YD-38 (human lower gingiva epithelial cell, KCLB 60508, Korea Cell Line Bank, Seoul), a lower gum epithelial cell line. Cell viability was evaluated.

YD-38 cells were grown in RPMI 1640 (Gibco BRL, USA) supplemented with 10% fetal bovine serum (Gibco BRL, USA) and 1% penicillin/streptomycin (Gibco BRL, USA) at 37°C in a 5% CO ₂ incubator. cultured.

YD-38 cells were dispensed in a 96-well plate at a concentration of 5 x 10 ³ cells/mL, and after incubation for 24 hours, samples diluted at various concentrations were treated.

After culturing for 24 hours, the culture medium was removed, 50 μl of MTT solution was added, followed by incubation for 3 hours, treatment with 200 μl of DMSO, and absorbance at 570 nm was measured.

As a result, as shown in FIG. 1, since higher bee venom content causes cytotoxicity and is disadvantageous in sensory evaluation, the optimal concentration having antibacterial efficacy and good sensory evaluation is 1 to 10 μg of purified bee venom per 10 mg of honey, most preferably. Preferably, it was suitable to contain 1 μg.

In addition, in order to confirm the antibacterial effect of purified bee venom and honey on S. mutans, S. sanguis, P. gingivalis and F. nucleatum , the present inventors used a liquid medium dilution method to obtain a minimum inhibitory concentration (MIC) was measured.

The purified bee venom was diluted with sterile distilled water, filtered aseptically, and gradually diluted according to the liquid medium dilution method.

Each test strain was pre-cultured in a liquid medium, adjusted to 2 x 10 ⁶ CFU/well of the inoculum, and incubated with the sample for 18 hours, and then the growth of the strain was observed with the naked eye and under a microscope. At this time, the absorbance (Molecular device spectramax M2e, CA) was measured at an absorption wavelength of 540 nm, and the same result as the absorbance value of the pure culture medium was determined as the minimum inhibitory concentration.

As a result, as shown in Table 2 below, it was confirmed that the purified bee venom and honey exhibited excellent antibacterial activity against various oral disease-causing strains.

oral disease causing bacteria Purified bee venom (μg/ml) Honey (μg/ml) Minimum growth inhibitory concentration (μg/ml) Streptococcus mutans 1.34 ± 0.3 125 3.57 Streptococcus sanguis 1.67 ± 0.3 255 4.47 Porphyromonas gingivalis 8.5 ± 0.9 > 500 10.9 Fusobacterium nucleatum 6.8 ± 0.6 > 500 8.7

Example 2. Evaluation of the inhibitory effect on bad breath of purified bee venom and honey

The present inventors measured the inhibitory effect on methylmercaptan, the main component of bad breath, using a halimeter to confirm the effect of suppressing bad breath according to the ratio of purified bee venom and honey.

After diluting the methyl mercaptan standard solution in 0.2 M potassium phosphate buffer solution to obtain a halitometer measurement value of 500-600 ppb, the diluted solution was used as a halitosis inducing solution. The purified bee venom and honey samples were dissolved in 0.2M potassium phosphate buffer solution, respectively, and used as a 1% solution. As a control, 500 μl of the buffer solution and 500 μl of the halitosis inducer solution were added to the test tube. The test tube was incubated in a 36.5 ° C water bath for 5 minutes and then measured with a halometer. The degree of inhibition of halitosis components for each 1% solution of each test group was determined.

As a result, as shown in Table 3 below, the halitosis inhibitory effect was attributed to honey. However, if the honey content is high, dilution with bee venom becomes difficult and the sweetness is too strong, so it was appropriate not to exceed 100 mg.

manufacturing example Purified bee venom (μg) honey (mg) Bad breath suppression degree (%) One 0 10 70 2 10 0 15 3 0.1 10 71 4 One 10 75 5 10 10 76 6 One 100 75

Example 3. Preparation of natural edible antibacterial film containing purified bee venom and honey

Based on the results described in Examples 1 and 2, the present inventors prepared a natural edible antibacterial film effective in removing tooth decay and bad breath using a mixture of purified bee venom and honey (containing 1 μg of purified bee venom per 10 mg of honey).

Briefly, with the composition and content of additives as shown in Table 4 below, the film-forming agent was dissolved in purified water, and then vitamins, a thickener and a wetting agent were added and stirred. A slurry was prepared by adding an emulsifier to a mixture of purified bee venom and honey, and then diluted with the prepared aqueous solution and mixed in a water bath at 60°C.

The film-forming composition was prepared into a film by spreading and pouring the prepared solution on a tray and then drying it with hot air.

As a result, Preparation Examples 9 to 12 were well formed in the form of films.

Content (mg) Preparation Example 7 Preparation Example 8 Preparation Example 9 Production Example 10 Preparation Example 11 Production Example 12 content % content % content % content % content % content % Bee Venom and Honey Mixture 10 2 10 2 0.1 0.02 One 0.2 10 2 100 20 Hydroxypropylmethylcellulose 20 4 20 4 20 4 20 4 20 4 20 4 pectin 10 2 10 2 10 2 10 2 10 2 10 2 pullulan 10 2 0 0 5 One 5 One 5 One 5 One carrageenan 0 0 10 2 5 One 5 One 5 One 5 One modified starch 10 2 10 2 10 2 10 2 10 2 10 2 glycerin 10 2 10 2 10 2 10 2 10 2 10 2 vitamin C 5 One 5 One 5 One 5 One 5 One 5 One CMC-Ca 4 0.8 4 0.8 4 0.8 4 0.8 4 0.8 4 0.8 propylene glycol 4 0.8 4 0.8 4 0.8 4 0.8 4 0.8 4 0.8 tamarind gum 3 0.6 3 0.6 3 0.6 3 0.6 3 0.6 3 0.6 Vitamin E 50% mixed formulation 2 0.4 2 0.4 2 0.4 2 0.4 2 0.4 2 0.4 polysorbate 80 4 0.8 4 0.8 4 0.8 4 0.8 4 0.8 4 0.8 Purified water 408 81.6 408 81.6 317.9 63.58 417 83.4 408 81.6 318 63.6 total 500 100 500 100 400 80 500 100 500 100 500 100

Example 4. Sensory evaluation of natural edible antibacterial film containing purified bee venom and honey

The present inventors performed sensory evaluation on texture, taste and aroma, convenience and preference of the orally disintegrating natural edible antibacterial film prepared in Example 3, and the results are shown in Table 6 below.

The sensory test was conducted on 30 general consumers, and the score and evaluation criteria used a 9-point scoring method, and are shown in Table 5 below.

score Evaluation standard 9 very good 7 good 5 commonly 3 bad One very bad

division texture taste or smell convenience Overall Preference Preparation Example 7 5 9 5 6.3 Preparation Example 8 5 9 7 7 Preparation Example 9 9 7 9 8.3 Preparation Example 10 9 9 9 9 Preparation Example 11 9 9 9 9 Preparation Example 12 5 9 5 6.3

As shown in Table 6, as a result of comparing the texture, taste and aroma, convenience and preference of the oral disintegrating films for removing bad breath prepared in Preparation Examples 7 to 12, the oral disintegrating film for removing bad breath of the present invention It was confirmed that the texture, taste and aroma, convenience and preference of the were excellent.

This is because the oral disintegrating film for removing bad breath manufactured as in the example can be used by dissolving after being attached to the oral cavity, so that the liquid mouthwash rinsed after use is inconvenient to spit out the mouth. Conventional liquid mouthwash It is judged that the texture and convenience are significantly increased to satisfy the preference of consumers.

Example 5. Evaluation of anti-halitosis inhibition of natural edible antibacterial film containing purified bee venom and honey

The present inventors evaluated the effect of suppressing bad breath of the orally disintegrating natural edible antibacterial film prepared in Example 3.

In order to conduct an experiment on the effect of suppressing bad breath of oral disintegrating film for removing bad breath, 20 men and women who did not have dental caries were selected as test subjects. The halitosis inhibition of the test subjects was measured using a halometer device.

In the halitosis suppression effect experiment, the degree of bad breath was determined by measuring the bad breath with a halometer after eating the same meal. Thereafter, the oral disintegrating film for removing bad breath prepared according to the preparation example was attached to the oral cavity to be dissolved, and then the bad breath was measured again with a halometer.

After applying the oral disintegrating film for removing bad breath prepared according to Preparation Example, the results on the effect of suppressing bad breath are shown in Table 7 below.

division Average halitosis suppression rate (%) Preparation Example 7 93 Preparation Example 8 92 Preparation Example 9 65 Preparation Example 10 75 Preparation Example 11 93 Preparation Example 12 95

Referring to Table 7, the oral disintegrating film for removing bad breath prepared according to Preparation Example 7 and Preparation Example 12 showed a high rate of suppression of bad breath in general, and among them, in particular, oral shelf for removing bad breath prepared according to Preparation Example 12. Haefilm showed a bad breath suppression rate of 95%, and it was determined that the bad breath suppression effect was enhanced by the composition ratio.

Although the preferred embodiment of the present invention has been described above, those skilled in the art will understand that the present invention can be implemented in other specific forms without changing the technical spirit or essential features. You will be able to. Therefore, one embodiment described above should be understood as illustrative in all respects and not limiting.

Claims (11)

A mixture of bee venom and honey as active ingredients; And a natural edible antibacterial film composition for preventing or improving bad breath, tooth decay or periodontal disease, comprising pullulan and carrageenan as film forming agents,
The mixture of bee venom and honey contains 1 μg of purified bee venom per 10 mg of honey;
The mixture of bee venom and honey is contained in an amount of 2% by weight based on the composition;
The pullulan is contained in 1% by weight based on the composition;
The carrageenan is a natural edible antibacterial film composition for preventing or improving halitosis, tooth decay or periodontal disease, characterized in that it is contained in an amount of 1% by weight based on the composition.
delete According to claim 1,
Characterized in that the bee venom is a purified bee venom isolated from the bee venom of a western bee ( Apis mellifera L.) worker bee,
A natural edible antibacterial film composition for preventing or improving halitosis, tooth decay or periodontal disease.
According to claim 1,
The composition is one selected from the group consisting of Streptococcus mutans , Streptococcus sanguis , Porphyromonas gingivalis , and Fusobacterium nucleatum Characterized in that it exhibits antibacterial activity against the above microorganisms,
A natural edible antibacterial film composition for preventing or improving halitosis, tooth decay or periodontal disease.
According to claim 1,
The natural edible antibacterial film composition further comprises at least one selected from the group consisting of solvents, vitamins, thickeners, wetting agents, emulsifiers, pH adjusting agents, nutrients, lubricants, flavoring agents, suspending agents, preservatives, and surfactants. characterized by,
A natural edible antibacterial film composition for preventing or improving halitosis, tooth decay or periodontal disease.
delete delete delete delete A method for producing a natural edible antibacterial film for preventing or improving halitosis, tooth decay or periodontal disease, comprising the following steps:
(a) preparing an edible film composition solution by dissolving pullulan and carrageenan as a film forming agent in a solvent, adding vitamins, a thickener and a wetting agent and stirring;
(b) preparing an emulsion slurry by mixing an emulsifier with a mixture of bee venom and honey,
The mixture of bee venom and honey contains 1 μg of purified bee venom per 10 mg of honey,
(c) preparing a natural edible antibacterial film composition by mixing the edible film composition solution of step (a) and the emulsion slurry containing the mixture of bee venom and honey of step (b) in a water bath at 60 ° C,
The mixture of bee venom and honey is contained in an amount of 2% by weight based on the natural edible antibacterial film composition;
The pullulan is contained in an amount of 1% by weight based on the natural edible antibacterial film composition;
The carrageenan is contained in an amount of 1% by weight based on the natural edible antibacterial film composition; and
(d) casting the natural edible antibacterial film composition of (c), molding it into a film form, and drying it with hot air. delete