KR102575768B1 - Natural Edible Film Compositions for Preventing or Improving Oral Diseases and Uses Thereof - Google Patents
Natural Edible Film Compositions for Preventing or Improving Oral Diseases and Uses Thereof Download PDFInfo
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- KR102575768B1 KR102575768B1 KR1020200164293A KR20200164293A KR102575768B1 KR 102575768 B1 KR102575768 B1 KR 102575768B1 KR 1020200164293 A KR1020200164293 A KR 1020200164293A KR 20200164293 A KR20200164293 A KR 20200164293A KR 102575768 B1 KR102575768 B1 KR 102575768B1
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- South Korea
- Prior art keywords
- bee venom
- honey
- film composition
- natural edible
- antibacterial film
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- 210000000056 organ Anatomy 0.000 description 1
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- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/0204—Specific forms not provided for by any of groups A61K8/0208 - A61K8/14
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/987—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of species other than mammals or birds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/987—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of species other than mammals or birds
- A61K8/988—Honey; Royal jelly, Propolis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D65/00—Wrappers or flexible covers; Packaging materials of special type or form
- B65D65/38—Packaging materials of special type or form
- B65D65/46—Applications of disintegrable, dissolvable or edible materials
- B65D65/463—Edible packaging materials
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2250/00—Food ingredients
- A23V2250/20—Natural extracts
- A23V2250/204—Animal extracts
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2250/00—Food ingredients
- A23V2250/60—Sugars, e.g. mono-, di-, tri-, tetra-saccharides
Abstract
본 발명은 구취, 충치 또는 치주질환의 예방 또는 개선용 천연 가식성 항균 필름 조성물 및 이의 용도에 관한 것으로, 유효성분으로 봉독 및 벌꿀의 혼합물; 및 필름형성제를 포함하는, 구취, 충치 또는 치주질환의 예방 또는 개선용 천연 가식성 항균 필름 조성물에 관한 것이다.
본 발명에 의한 천연 항균 물질로 만들어진 가식성 항균필름 조성물에 의하면, 인체에 부작용이 전혀 없을 뿐만 아니라, 필름에 포함된 정제봉독 및 벌꿀의 구취 유발 물질 제거 효능 및 구강 질환 유발균에 대한 항균 작용에 의해서, 구강 질환 유발을 억제하며, 장시간 동안 구취제거가 가능하여 소비자가 간편하게 휴대, 사용할 수 있는 새로운 개념의 구강 청결유지 및 충치생성방지 제품으로 사용될 수 있으며, 식품 포장용 항균필름 등으로 이용될 수 있다.The present invention relates to a natural edible antibacterial film composition for preventing or improving halitosis, tooth decay or periodontal disease and its use, comprising a mixture of bee venom and honey as active ingredients; And it relates to a natural edible antibacterial film composition for preventing or improving halitosis, tooth decay or periodontal disease, including a film forming agent.
According to the edible antibacterial film composition made of natural antibacterial substances according to the present invention, there is no side effect on the human body, and the purified bee venom and honey contained in the film are effective in removing odor-causing substances and have antibacterial action against oral disease-causing bacteria. As a result, it can be used as a new concept of oral hygiene maintenance and tooth decay prevention product that can be easily carried and used by consumers by suppressing oral disease and removing bad breath for a long time, and can be used as an antibacterial film for food packaging. .
Description
본 발명은 구강 질환, 바람직하게는 구취, 충치 또는 치주질환 예방 또는 개선용 천연 가식성 항균 필름 조성물 및 이의 용도에 관한 것이다.The present invention relates to a natural edible antibacterial film composition for preventing or improving oral diseases, preferably halitosis, tooth decay or periodontal disease, and its use.
현대 사회는 산업화 및 서구화가 됨에 따라 생활습관과 식생활의 변화로 구강관련 질환의 원인과 증상이 다양해지고, 심각한 사회문제로 보고되고 있다. WHO에서는 치아우식(충치), 치주질환, 구취 및 구강 통증과 같은 구강 질환을 미래에 해결해야 할 주요 보건문제 중 하나로 인식하고 있다. WHO의 치과질환에 관한 보고에 따르면, 전 세계적 청소년 60~90%와 성인 60%가 치아우식과 치주질환을 겪고 있으며, 중년성인의 15 ~ 20%가 심한 치주 질환을 가지고 있다. 이러한 구강관련 질환은 치아 손실의 주요 원인이 될 뿐만 아니라, 심혈관계 질환, 세균성 폐렴, 저체중 출생아, 당뇨합병증과 골다공증 같은 질병의 잠재적인 위험요소가 되는 것으로 보고되고 있다.As modern society becomes industrialized and westernized, the causes and symptoms of oral-related diseases are diversified due to changes in lifestyle and dietary habits, and are reported as serious social problems. Oral diseases such as dental caries (cavities), periodontal disease, halitosis and oral pain are recognized by WHO as one of the major health problems to be addressed in the future. According to the WHO report on dental disease, 60-90% of adolescents and 60% of adults worldwide suffer from dental caries and periodontal disease, and 15-20% of middle-aged adults have severe periodontal disease. These oral diseases are reported to be not only a major cause of tooth loss, but also a potential risk factor for diseases such as cardiovascular diseases, bacterial pneumonia, low birth weight infants, complications of diabetes, and osteoporosis.
특히, 최근 사회생활의 중요한 대인관계의 장애가 되는 구취에 관한 이슈가 대두하고 있으며, 그에 따라 구강건강에 관한 관심이 증가하고 있다.In particular, the issue of bad breath, which is an important interpersonal obstacle in social life, has recently emerged, and accordingly, interest in oral health is increasing.
구취는 후천적인 질병에 의해 발생하기도 하나, 일반적으로는 타액 중의 단백질, 음식물 찌꺼기 등이 구강 내에서 미생물에 의해 분해되어 생성된 아미노산이 탈회산 효소나 아미노 효소 등에 의해 분해되어 악취를 유발하는 물질을 생성함으로써 발생한다. 또한, 마늘이나 고추 등을 섭취하는 경우 이들 물질에 함유되어 있는 황화물에 의해서도 구취가 발생하게 된다.Bad breath is sometimes caused by acquired diseases, but in general, proteins and food waste in saliva are decomposed by microorganisms in the oral cavity and amino acids are decomposed by demineralization enzymes or amino enzymes to produce substances that cause odor. occurs by creating In addition, when garlic or red pepper is ingested, bad breath also occurs due to sulfides contained in these substances.
이러한 구취를 유발하는 대표적인 성분으로는 휘발성 황화물(Volatile Sulfide Compounds; VSC)을 들 수 있는데, 이들 휘발성 황화물에는 황화수소(Hydrogen sulfide, H2S), 메틸머캅탄(Methyl mercaptan, CH3SH), 디메틸머캅탄(Dimethyl mercaptan) 등이 있으며, 특히 메틸머캅탄은 구취 중 역겨운 냄새의 주성분으로 알려지고 있다. 또한, 휘발성 아민화합물, 예를 들어, 트리메틸아민(Trimethyl amine) 또는 δ-아미노발레린산(δ-Aminovaleric acid) 등과 같은 휘발성 아민화합물도 악취성분의 주요한 성분으로 알려지고 있으며, 이외에도 구취 유발 물질로는 알데히드, 지방산, 암모니아 또는 피리딘 등과 같은 성분들을 들 수 있다.Representative components that cause such halitosis include volatile sulfide compounds (VSC). These volatile sulfides include hydrogen sulfide (H 2 S), methyl mercaptan (CH 3 SH), dimethyl There are mercaptans (Dimethyl mercaptan) and the like, and methyl mercaptan in particular is known as the main component of the disgusting smell among bad breath. In addition, volatile amine compounds, such as trimethyl amine or δ-aminovaleric acid, are also known as major components of odor components. components such as aldehydes, fatty acids, ammonia or pyridine.
이러한 구취의 원인으로는 구강내 원인, 구강외 원인 및 심인적 원인으로 크게 분류할 수 있는데, 그 중 구강내 원인이 구취원인의 80~90% 정도를 차지하고, 상기 구강내 원인으로는 타액량 감소, 타액점조도 증가, 수소이온 농도 완충능력 감소, 구강 미생물의 양과 활동성 증가 및 혀의 배면에 부착된 설태와 치료되지 않은 치아우식증과 치주질환과 같은 구강 질환 등을 들 수 있다.Causes of bad breath can be largely classified into oral causes, extraoral causes, and psychogenic causes. , increase in salivary consistency, decrease in hydrogen ion concentration buffering capacity, increase in the amount and activity of oral microorganisms, and oral diseases such as tongue plaque attached to the back of the tongue and untreated dental caries and periodontal disease.
한편, 상기와 같이 구취의 주요원인 중 하나인 치아우식, 치주질환 및 구취를 유발하는 구강병원균 증식을 억제하는 방법으로, 이들 세균들에 대한 살균 및 정균작용을 갖는 항생제를 포함한 다양한 종류의 항균 제제가 충치, 치주질환, 치수 및 치근단 감염의 억제 및 치료제로 개발되었다.On the other hand, as described above, as a method of inhibiting the growth of oral pathogens that cause dental caries, periodontal disease, and bad breath, which are one of the main causes of bad breath, various types of antibacterial agents including antibiotics having bactericidal and bacteriostatic action against these bacteria was developed as an inhibitor and treatment for tooth decay, periodontal disease, pulp and apical infection.
그러나 상기와 같이 항생제 등과 같은 구강질환 치료제는 인체 전반에 대한 부작용과 함께 구강 내 내성균의 출현 및 균교대증을 유발할 수 있기 때문에 장기적인 사용이 곤란하여 단지 치료제만으로 이용될 수 있는 문제가 있었다.However, oral disease treatment such as antibiotics as described above has a problem that can be used only as a treatment because long-term use is difficult because it can cause the appearance of resistant bacteria in the oral cavity and mycosis, along with side effects on the human body as a whole.
한편, 구강내 세균 증식 또는 음식물의 부패에 의해 발생하는 구취를 제거하고 구강의 청결을 유지하기 위한 목적으로 단순히 구강을 헹구어 세척하는 액상형 구강청정제가 개발되었는데, 상기 액상형 구강청정제는 그 자체의 중량이 크고 부피가 크며 게다가 부수적으로 따르는 컵과 같은 용기도 필요한 경우가 대부분이어서 휴대하기가 불편한 단점이 있으며, 입안을 헹구어 내는 방법으로 입안의 구취를 제거할 수 있으나, 사용 후 입안을 헹군 액상형 구강청정제를 외부로 뱉어내야 하는 등의 불편함이 있었다.On the other hand, for the purpose of removing bad breath caused by the growth of bacteria in the mouth or spoilage of food and maintaining oral cleanliness, a liquid-type mouthwash has been developed that simply rinses and cleans the mouth. The liquid-type mouthwash has a weight of its own. It is large and bulky, and in most cases, a container such as an additional cup is required, so it is inconvenient to carry, and bad breath can be removed by rinsing the mouth. There was such an inconvenience as having to spit it out.
따라서, 사용이 편리하여 언제 어디서든 간편하게 사용 가능하고 식사 후 또는 공복시 발생하는 입안의 냄새를 효과적으로 제거하며 부작용에 대한 우려 없이 인체에도 안전하여 안심하고 사용할 수 있는 구취제거제에 대한 개발이 요구되고 있는 실정이다.Therefore, it is convenient to use, can be used anytime, anywhere, effectively removes the smell in the mouth that occurs after eating or on an empty stomach, and is safe for the human body without worrying about side effects. am.
최근 들어 단백질, 탄수화물 및 지방 등의 생고분자를 기초로 하는 가식성 필름을 이용하는 연구가 활발하게 이루어지고 있다. 이 필름은 비식용, 비분해성 플라스틱 고분자 필름에 비해 쉽게 분해되며, 향미, 색소, 감미료 등의 성분을 가식성 필름에 첨가하여 내용물에 관능적(organoleptic) 특성을 부여할 수 있으며, 부족 영양소를 포함한 식용 필름으로 사용할 수 있고, 물질 이동 차단재로 이용될 수 있는 등 식품포장재로 사용될 수 있다.Recently, studies using edible films based on biopolymers such as proteins, carbohydrates, and fats have been actively conducted. This film is easily degraded compared to non-edible, non-degradable plastic polymer film, and ingredients such as flavors, colors, and sweeteners can be added to the edible film to give organoleptic characteristics to the contents, and to be edible including deficient nutrients. It can be used as a film, can be used as a food packaging material, such as being used as a material transfer barrier.
이러한 상황 하에서, 본 발명자들은, 필요시 항균 및 항충치 가식성 필름을 섭취하여 사용함으로써, 가식성 필름이 용해되어 장시간 동안 약효물질을 방출시켜, 구취제거에 부작용 없이 효과적으로 사용할 수 있고, 더 나아가 구강 질환을 개선할 수 있는 구취제거제를 개발하고자 예의 연구노력하였다. 그 결과, 본 발명자들은 인체에 무해한 천연물질로서 정제 봉독 및 벌꿀을 이용하여 입안에서 쉽게 용해되며 오래 머무를 수 있는 필름 형태의 구취제거제를 제조하였고, 이를 이용하는 경우, 정제 봉독 및 벌꿀의 유효 성분을 용이하게 섭취할 수 있고, 구취를 효과적으로 제거하여 구강의 청결을 유지할 수 있을 뿐만 아니라, 구강내 충치 유발균에 의한 충치를 억제할 수 있음을 규명함으로써, 본 발명을 완성하였다.Under these circumstances, the inventors of the present invention, by ingesting and using the antibacterial and anti-caries edible film when necessary, the edible film is dissolved and the medicinal substance is released for a long time, so that it can be effectively used for removing bad breath without side effects, and furthermore, oral Research efforts were made to develop a bad breath remover that could improve the disease. As a result, the inventors of the present invention prepared a halitosis remover in the form of a film that can be easily dissolved in the mouth and can stay for a long time using purified bee venom and honey, which are natural substances harmless to the human body. Not only can it be consumed in a reasonable way, it can effectively remove bad breath to maintain oral cleanliness, and it can also prevent tooth decay caused by bacteria that cause tooth decay in the oral cavity. The present invention was completed by clarifying that it can be suppressed.
따라서, 본 발명의 일 목적은, 구취, 충치 또는 치주질환의 예방 또는 개선용 천연 가식성 항균 필름 조성물을 제공하는 데 있다.Accordingly, one object of the present invention is to provide a natural edible antibacterial film composition for preventing or improving halitosis, tooth decay or periodontal disease.
또한, 본 발명의 다른 목적은, 구취, 충치 또는 치주질환의 예방 또는 개선용 식품 조성물을 제공하는 데 있다.Another object of the present invention is to provide a food composition for preventing or improving bad breath, tooth decay or periodontal disease.
또한, 본 발명의 또 다른 목적은, 구취, 충치 또는 치주질환의 예방 또는 개선용 의약외품 조성물을 제공하는 데 있다.Another object of the present invention is to provide a quasi-drug composition for preventing or improving halitosis, tooth decay or periodontal disease.
또한, 본 발명의 또 다른 목적은 구취, 충치 또는 치주질환의 예방 또는 개선용 천연 가식성 항균 필름 조성물을 제조하는 방법을 제공하는 데 있다.Another object of the present invention is to provide a method for preparing a natural edible antibacterial film composition for preventing or improving halitosis, tooth decay or periodontal disease.
본 발명의 다른 목적 및 이점은 하기의 발명의 상세한 설명, 청구범위 및 도면에 의해 보다 명확하게 된다.Other objects and advantages of the present invention will become more apparent from the following detailed description of the invention, claims and drawings.
본 명세서에서 사용한 용어는 단지 설명을 목적으로 사용된 것으로, 한정하려는 의도로 해석되어서는 안된다. 단수의 표현은 문맥상 명백하게 다르게 뜻하지 않는 한, 복수의 표현을 포함한다. 본 명세서에서, "포함하다" 또는 "가지다" 등의 용어는 명세서 상에 기재된 특징, 숫자, 단계, 동작, 구성요소, 부품 또는 이들을 조합한 것이 존재함을 지정하려는 것이지, 하나 또는 그 이상의 다른 특징들이나 숫자, 단계, 동작, 구성요소, 부품 또는 이들을 조합한 것들의 존재 또는 부가 가능성을 미리 배제하지 않는 것으로 이해되어야 한다.The terms used herein are used for descriptive purposes only and should not be construed as limiting. Singular expressions include plural expressions unless the context clearly dictates otherwise. In this specification, terms such as "include" or "have" are intended to designate that there is a feature, number, step, operation, component, part, or combination thereof described in the specification, but one or more other features It should be understood that the presence or addition of numbers, steps, operations, components, parts, or combinations thereof is not precluded.
다르게 정의되지 않는 한, 기술적이거나 과학적인 용어를 포함해서 여기서 사용되는 모든 용어들은 실시예가 속하는 기술 분야에서 통상의 지식을 가진 자에 의해 일반적으로 이해되는 것과 동일한 의미를 가지고 있다. 일반적으로 사용되는 사전에 정의되어 있는 것과 같은 용어들은 관련 기술의 문맥 상 가지는 의미와 일치하는 의미를 가지는 것으로 해석되어야 하며, 본 출원에서 명백하게 정의하지 않는 한, 이상적이거나 과도하게 형식적인 의미로 해석되지 않는다.Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by a person of ordinary skill in the art to which the embodiment belongs. Terms such as those defined in commonly used dictionaries should be interpreted as having a meaning consistent with the meaning in the context of the related art, and unless explicitly defined in the present application, they should not be interpreted in an ideal or excessively formal meaning. don't
이하, 본 발명에 대하여 보다 상세히 설명한다.Hereinafter, the present invention will be described in more detail.
본 발명의 일 양태에 따르면, 본 발명은 유효성분으로 봉독 및 벌꿀의 혼합물; 및 필름형성제를 포함하는, 충치, 구취 또는 치주질환의 예방 또는 개선용 천연 가식성 항균 필름 조성물을 제공한다.According to one aspect of the present invention, the present invention provides a mixture of bee venom and honey as an active ingredient; And it provides a natural edible antibacterial film composition for preventing or improving tooth decay, halitosis or periodontal disease, including a film forming agent.
본 발명에 있어서, "구강 질환"이란 구강 영역에서 발생하는 여러 가지 질환을 말하며, 상기 구강영역은 앞쪽 입술부터 뒤쪽 구협에서 인두와 연결되는 입 안의 공간을 의미한다. 본 발명에서 상기 구강 질환은 구강에 발생하는 질환이라면 그 병증에 관계없이 모두 포함하는 개념이며, 상기 구강질환의 비제한적인 예로는 치아 우식증(충치), 치주질환(치주염 또는 치은염), 구취, 치통, 시린이 등을 들 수 있다.In the present invention, "oral disease" refers to various diseases occurring in the oral region, and the oral region refers to the space in the mouth connected to the pharynx from the front lip to the back oral cavity. In the present invention, the oral disease is a concept that includes all diseases that occur in the oral cavity regardless of the disease, and non-limiting examples of the oral disease include dental caries (cavities), periodontal disease (periodontitis or gingivitis), bad breath, toothache , Shirin, etc.
본 발명의 용어, "충치"는 벌레 먹은 치아라는 뜻으로 치아의 경조직이 침식되어 결손하는 증세이며 우식증(陶蝕症)이라고도 한다. 충치는 치아의 딱딱한 석회화 조직의 일부가 용해되고 파괴되는 통증을 유발하고 결국에는 치아를 잃게 되는 감염성 세균 질환으로, 치주질환 가운데 가장 많다.The term of the present invention, "cavity" means worm-eaten teeth, and is a condition in which the hard tissues of the teeth are eroded and lost, and is also called caries. Tooth decay is an infectious bacterial disease in which a part of the hard calcified tissue of the tooth is dissolved and destroyed, causing pain and eventually causing tooth loss, and is the most common periodontal disease.
본 발명의 용어, "치주질환"은 흔히 풍치라고도 하는데, 병의 정도에 따라 치은염(gingivitis)과 치주염(periodontitis)으로 나뉜다. 비교적 가볍고 회복이 빠른 형태의 구강질환으로 잇몸 즉, 연조직에만 국한된 형태를 치은염이라고 하고, 이러한 염증이 치은(gingiva)과 치조골(alveolar bone) 주변까지 진행된 경우를 치주염이라고 한다.The term of the present invention, "periodontal disease" is often referred to as periodontal disease, and is divided into gingivitis and periodontitis according to the severity of the disease. Gingivitis is a relatively mild and reversible oral disease limited to the gums, that is, soft tissues, and periodontitis is when this inflammation progresses to the gingiva and around the alveolar bone.
치은(잇몸)과 치아 사이에는 V자 모양의 틈이 있는데, 이 치은 열구(gingival sulcus)의 치은 아래 부분을 박테리아가 공격하여 치주인대와 인접조직을 손상시키는 것이 치주질환이다. 염증이 진행되어 더 많은 조직이 손상되면서 홈이 치주낭(periodontal pocket)으로 발전하게 되며, 치주염이 심할수록 치주낭의 깊이가 깊어지게 된다. 치주낭이 깊어지면서 치주인대에 염증이 생기게 되고, 포르피로모나스 진지발리스에 의해 분비되는 리포다당류(lipopolysaccharide; LPS)에 의해 파골세포 분화가 촉진되어 치조골 소실이 일어나게 되는 것이 치주질환이다.There is a V-shaped gap between the gingiva (gum) and the tooth, and periodontal disease is when bacteria attack the lower gingival portion of this gingival sulcus, damaging the periodontal ligament and adjacent tissues. As inflammation progresses and more tissues are damaged, the groove develops into a periodontal pocket, and as periodontitis is severe, the depth of the periodontal pocket deepens. As the periodontal pocket deepens, the periodontal ligament becomes inflamed, and osteoclast differentiation is promoted by lipopolysaccharide (LPS) secreted by Porphyromonas gingivalis, resulting in alveolar bone loss.
본 발명에서 용어, "구취"는 구강 및 인접기관으로부터 유래되는 냄새로 구취의 85 - 90%가 구강에서 유래하며, 특히, 혀의 뒷쪽에서 유래하고 있다. 구취의 주요 성분은 휘발성 황화합물인데, 휘발성 황화합물의 전체량 중 90%가 시스테인으로부터 만들어지는 황화수소(hydrogen sulfide)와 메티오닌으로부터 만들어지는 메틸머캡탄(methyl mercaptan)및 디메틸설파이드(dimethyl sulfide)이다. 이러한 성분들은 주로 혐기성 세균이 분비하는 단백질 효소에 의해서 생성되며, 혀의 뒷쪽이 가장 중요한 서식지가 된다. 이 부위는 타액에 의해 세정작용이 잘 되지 않고, 많은 작은 함몰이 있어 세균이 지속적으로 살아가는 장소가 된다. 혐기성 세균에 의한 휘발성 황화합물 생성이 구취의 원인으로 가장 중요하지만, 그 외 치아 우식증, 치주염, 구강 건조증 등과 같은 구강질환에 의해서도 발생한다. 구취를 발생시키는데 많은 종류의 혐기성 세균이 관여하며, 구취 발생 원인균의 비제한적인 예로 많은 종류와 많은 양의 효소를 분비하는 포르피로모나스 진지발리스(Porphyromonas gingivalis)를 들 수 있다.In the present invention, the term "halitosis" is a smell derived from the oral cavity and adjacent organs, and 85 to 90% of bad breath originates from the oral cavity, particularly from the back of the tongue. The main component of bad breath is volatile sulfur compounds, 90% of which are hydrogen sulfide made from cysteine and methyl mercaptan and dimethyl sulfide made from methionine. These components are mainly produced by protein enzymes secreted by anaerobic bacteria, and the back of the tongue becomes the most important habitat. This area is not well cleaned by saliva and has many small depressions, making it a place where bacteria live continuously. The production of volatile sulfur compounds by anaerobic bacteria is the most important cause of bad breath, but other oral diseases such as dental caries, periodontitis, and dry mouth are also caused. Many types of anaerobic bacteria are involved in generating bad breath, and a non-limiting example of bad breath causing bacteria is Porphyromonas gingivalis , which secretes many types and large amounts of enzymes.
상기의 목적을 달성하기 위한 것으로, 상기 유효성분인 정제봉독 및 벌꿀의 혼합물 외에 필름형성제, 용매, 비타민, 증점제, 습윤제 및 유화제를 포함하는 가식성 필름 조성물로서, 바람직하게는 상기 봉독 및 벌꿀의 혼합물은 상기 벌꿀 10mg 당 봉독 1 내지 10㎍이 함유될 수 있고; 상기 정제봉독 및 벌꿀의 혼합물은 전체 필름 조성물 중 0.001 내지 50 중량%되도록 혼합되어 구강 내에서 붕해되는, 구취, 충치 또는 치주질환의 예방 또는 개선용 천연 가식성 항균필름 조성물을 제공한다.In order to achieve the above object, an edible film composition comprising a film forming agent, a solvent, a vitamin, a thickener, a wetting agent and an emulsifier in addition to a mixture of purified bee venom and honey, which are the active ingredients, is preferably composed of the bee venom and honey. The mixture may contain 1 to 10 μg of bee venom per 10 mg of the honey; The mixture of the purified bee venom and honey is mixed at 0.001 to 50% by weight of the total film composition to provide a natural edible antibacterial film composition for preventing or improving halitosis, tooth decay or periodontal disease, which disintegrates in the oral cavity.
즉, 본 명세서에서, "천연 가식성 항균필름"은 "구강붕해필름"을 의미하며, 당업계에 공지된 바의 의미를 그대로 따르는데, 구체적으로 구강(oral cavity) 내에서 용해, 분산 등 붕해시켜 복용할 수 있는 필름 제형을 말한다. 이러한 필름 제형은 혀 위에 놓아 녹이게 되나, 입천장, 설하, 입안 안뜰(buccal) 등에 부착하여 섭취할 수 있다.That is, in the present specification, "natural edible antibacterial film" means "oral disintegrating film", and follows the meaning as known in the art, specifically dissolving, dispersing, etc. in the oral cavity. It refers to a film dosage form that can be disintegrated and taken. Such a film formulation is placed on the tongue and dissolved, but can be ingested by attaching to the roof of the mouth, sublingually, or the inside of the mouth (buccal).
본 명세서에서, "천연 가식성 항균필름 조성물"은 구강붕해필름을 제조하기 위한 구성성분들을 포함하는 혼합물 형태를 의미하며, 구강붕해필름을 제조하기 위한 건조 공정 전의 혼합물 형태를 제한없이 포함한다.In the present specification, "natural edible antibacterial film composition" means a mixture form containing components for preparing an oral disintegrating film, and includes without limitation a mixture form before a drying process for preparing an oral disintegrating film. .
본 발명의 정제봉독 및 벌꿀은 본 조성물의 유효성분으로서, 구취의 주성분인 메틸머캅탄을 제거할 수 있으며, 구강 내 존재하는 구강 질환 유발 병원균에 대한 항균력이 우수하여, 구취 제거는 물론 충치나 치주질환과 같은 구강 질환을 예방 또는 치료하는 효과가 있다.The purified bee venom and honey of the present invention, as active ingredients of the present composition, can remove methyl mercaptan, the main component of bad breath, and have excellent antibacterial activity against oral disease-causing pathogens existing in the oral cavity, thereby eliminating bad breath as well as cavities and periodontal There is an effect of preventing or treating oral diseases such as diseases.
본 발명의 일 실시예에서, 정제봉독은 서양종꿀벌(Apis mellifera)의 일벌로부터 봉독을 획득한 후, 간이 정제 방법으로 정제하여 멜리틴 함량이 63 ± 3%인 것을 사용하으나, 본 발명의 목적을 달성할 수 있는 한, 이에 제한되는 것은 아니다.In one embodiment of the present invention, purified bee venom is obtained by obtaining bee venom from worker bees of Western species ( Apis mellifera ) and then purified by a simple purification method to use a melittin content of 63 ± 3%. As long as it can achieve, it is not limited thereto.
본 발명의 일 실시예에서, 벌꿀은 아카시아꿀을 사용하였으나, 본 발명의 목적을 달성할 수 있는 한, 이에 제한되는 것은 아니다.In one embodiment of the present invention, honey was used as acacia honey, but it is not limited thereto as long as it can achieve the object of the present invention.
상기 필름형성제는, 구강 붕해 필름을 형성하게 하는 고분자로, 본 발명의 목적을 달성할 수 있는 한, 임의의 필름형성제를 이용할 수 있다. 본 발명에서 상기 필름형성제로는 전분, 수용성 전분, 변성전분, 폴리비닐피롤리돈, 폴리비닐알코올, 플루란, 젤라틴, 구아검, 잔탄검, 폴리에틸렌글리콜, 아카시아 검, 아라비아 검, 폴리아크릴산, 아밀로오즈, 펙틴, 덱스트린, 키틴, 제라틴, 글리텐, 카제인 및 카라기난으로 이루어진 군에서 선택된 1 종을 단독으로 또는 2종 이상을 혼용하여 0.5 내지 35중량%, 바람직하게는 5 내지 25중량%를 첨가하여 사용될 수 있는데, 이에 한정되는 것은 아니며, 본 발명의 일 실시예에서는 1 내지 20중량%를 첨가한다. The film-forming agent is a polymer capable of forming an orally disintegrating film, and any film-forming agent may be used as long as it can achieve the object of the present invention. In the present invention, the film forming agent includes starch, water-soluble starch, modified starch, polyvinylpyrrolidone, polyvinyl alcohol, pullulan, gelatin, guar gum, xanthan gum, polyethylene glycol, gum acacia, gum arabic, polyacrylic acid, amyl 0.5 to 35% by weight, preferably 5 to 25% by weight of one selected from the group consisting of rose, pectin, dextrin, chitin, geratin, gluten, casein and carrageenan alone or in combination of two or more. It may be used by adding, but is not limited thereto, and in one embodiment of the present invention, 1 to 20% by weight is added.
상기 용매는 구강 붕해 필름을 형성하는 조성물들을 용해 또는 분산시키기 위하여 사용되는 것으로, 본 발명의 목적을 달성할 수 있는 한, 인체에 무해한 용매를 임의의 이용할 수 있다. 본 발명에서 상기 용매로는 물이 사용될 수 있고, 상기 물로는 정제수 또는 증류수가 사용될 수 있으며, 이에 한정되는 것은 아니나, 본 발명의 일 실시예에서는 정제수를 첨가한다.The solvent is used to dissolve or disperse the compositions forming the oral disintegrating film, and any solvent harmless to the human body may be used as long as the object of the present invention can be achieved. In the present invention, water may be used as the solvent, and purified water or distilled water may be used as the water, but is not limited thereto, but in one embodiment of the present invention, purified water is added.
본 발명의 목적을 달성할 수 있는 한, 임의의 습윤제를 이용할 수 있다. 상기 습윤제로서는 비결정성 소르비톨액, 폴리에틸렌글리콜류, 프로필렌글리콜, 글리세린 및 농글리세린으로 이루어진 군에서 선택된 어느 하나 이상의 물질이 사용될 수 있으나, 이에 한정되는 것은 아니며, 본 발명의 일 실시예에서는 0.001 내지 0.02중량%를 첨가한다.Any humectant can be used as long as the object of the present invention can be achieved. As the wetting agent, any one or more materials selected from the group consisting of amorphous sorbitol, polyethylene glycols, propylene glycol, glycerin and concentrated glycerin may be used, but is not limited thereto, and in one embodiment of the present invention, 0.001 to 0.02 weight % is added.
본 발명의 목적을 달성할 수 있는 한, 임의의 유화제를 이용할 수 있다. 상기 유화제로서는 통상 식품에 사용되는 유화제를 사용할 수 있는데, 하이드록시프로필 메틸 셀룰로스(Hydroxypropyl methyl Cellulose), 레시틴(Lecithin), 카제인 나트륨(Sodium Caseinate), 카제인(Casein), 알긴산암모늄(Ammonium Alginate), 타마린드검, 폴리소르베이트80, 자당지방산에스테르 및 글리세린지방산에스테르로 이루어진 군에서 선택된 어느 하나 이상의 물질이 사용될 수 있으며, 이에 한정되는 것은 아니나, 본 발명의 일 실시예에서는 0.001 내지 0.02중량%를 첨가한다.Any emulsifier can be used as long as the object of the present invention can be achieved. As the emulsifier, emulsifiers commonly used in food may be used. Hydroxypropyl methyl Cellulose, Lecithin, Sodium Caseinate, Casein, Ammonium Alginate, Tama At least one material selected from the group consisting of Lind gum, polysorbate 80, sucrose fatty acid esters and glycerin fatty acid esters may be used, but is not limited thereto, and in one embodiment of the present invention, 0.001 to 0.02% by weight is added. .
이 외에, 본 발명의 천연 가식성 항균 필름 조성물에는 본 발명의 목적을 벗어나지 않는 한도 내에서, 당업계에 공지된 pH 조절제, 영양제, 윤활제, 향미제, 현탁제, 보존제 및 계면활성제로 이루어진 군으로부터 선택되는 1 종 이상을 추가로 첨가할 수 있다.In addition, the natural edible antibacterial film composition of the present invention, within the limit that does not deviate from the object of the present invention, from the group consisting of pH adjusting agents, nutrients, lubricants, flavoring agents, suspending agents, preservatives and surfactants known in the art One or more selected types may be further added.
일 실시예에서, 본 발명은 필름형성제, 비타민, 증점제, 습윤제 및 잔량의 용매를 혼합하여 제조한 가식성 필름 조성물 용액에, 유화제, 정제봉독 및 벌꿀의 혼합물을 포함하는 유화액 슬러리를 첨가하여 60℃ 수조에서 혼합시켜 본 발명의 천연 가식성 항균 필름 조성물을 제조한다. 상기 필름형성 조성물은 트레이에 제조한 용액을 얇게 펴서 부은 후 열풍건조시켜 필름으로 제조한다.In one embodiment, the present invention provides an emulsion slurry containing a mixture of an emulsifier, purified bee venom, and honey to an edible film composition solution prepared by mixing a film forming agent, a vitamin, a thickener, a wetting agent, and the remaining amount of a solvent to form a 60 The natural edible antibacterial film composition of the present invention is prepared by mixing in a water bath at ° C. The film-forming composition is prepared into a film by spreading and pouring the prepared solution on a tray and then drying it with hot air.
본 발명의 구취, 충치 또는 치주질환의 예방 또는 개선을 위한 조성물은 유용성분으로서 정제 봉독 및 벌꿀을 이용하여 구취제거 효과를 알아보기 위하여 구취의 주성분인 메틸머캅탄 제거 실험 결과 탁월한 메틸머캅탄 제거 능력을 입증하였다. The composition for preventing or improving halitosis, tooth decay or periodontal disease of the present invention has excellent methyl mercaptan removal ability as a result of an experiment on removing methyl mercaptan, the main component of bad breath, to determine the effect of removing bad breath using purified bee venom and honey as useful ingredients. proved.
또한, 구강내 잔류하는 스트렙토코커스 뮤탄스(Streptococcus mutans), 스트렙토코커스 상귀스(Streptococcus sanguis), 포르피로모나스 진지발리스(Porphyromonas Gingivalis) 및 푸소박테륨 누클레아툼(Fusobacterium nucleatum)의 제거 능력을 실험한 결과 탁월하게 항균 효과를 발휘함을 확인할 수 있었다.In addition, Streptococcus mutans remaining in the oral cavity ( Streptococcus mutans ), Streptococcus sanguis ( Streptococcus sanguis ), Porphyromonas gingivalis ( Porphyromonas Gingivalis ) and Fusobacterium nucleatum ( Fusobacterium nucleatum ) The removal ability test As a result, it was confirmed that the antibacterial effect was excellent.
이때 유효성분인 정제봉독 및 벌꿀의 혼합물의 함량은 조성물 기준으로 50%중량 초과하여 과다하게 포함되면 관능성이 저하되고 세포독성을 초래할 수 있으며, 0.001%중량 미만으로 너무 적게 포함되면 항균성이 낮아 효능이 반감될 수 있으므로 유효성분은 20%중량 정도 첨가하는 것이 바람직하다.At this time, if the content of the mixture of purified bee venom and honey, which are active ingredients, is more than 50% by weight based on the composition, if it is included excessively, the sensory function may decrease and cytotoxicity may occur, and if it is included too little, less than 0.001% by weight, the antibacterial activity is low and the efficacy is low. Since this may be halved, it is preferable to add about 20% of the active ingredient by weight.
본 발명의 조성물은 유효성분인 봉독 및 벌꿀의 혼합물이 식품 소재인 점 등을 고려할 때 통상 식품으로 제조될 수 있다.The composition of the present invention can be prepared as a normal food, considering that the mixture of bee venom and honey, which are active ingredients, is a food material.
본 발명의 구강붕해필름 제형의 식품 조성물에 있어서, 봉독 및 벌꿀의 혼합물은 그것의 의도한 식품으로서의 기능성을 충분히 나타내고, 물성, 붕해성 등에 있어 필름 제형으로서의 적정성에 영향을 미치지 않는 한 임의의 양으로 포함될 수 있다.In the food composition of the oral disintegrating film dosage form of the present invention, the mixture of bee venom and honey is used in any amount as long as it sufficiently exhibits its intended functionality as a food and does not affect the suitability as a film dosage form in terms of physical properties, disintegration properties, etc. can be included as
본 발명의 조성물은 구취, 충치 또는 치주질환의 예방, 개선 또는 제거를 위한 의약외품용 조성물일 수 있다.The composition of the present invention may be a quasi-drug composition for preventing, improving or removing halitosis, tooth decay or periodontal disease.
본 발명의 봉독 및 벌꿀의 혼합물을 의약외품 첨가물로 사용할 경우, 구취제거 활성이 있는 상기 혼합물의 유효성분을 그대로 첨가하거나 다른 의약외품 또는 의약외품 성분과 함께 사용될 수 있고, 통상적인 방법에 따라 적절하게 사용될 수 있다. 유효 성분의 혼합양은 사용 목적에 따라 적합하게 결정될 수 있다.When the mixture of bee venom and honey according to the present invention is used as a quasi-drug additive, the active ingredient of the mixture having a bad breath removal activity can be added as it is or used together with other quasi-drugs or quasi-drug ingredients, and can be appropriately used according to a conventional method. . The mixing amount of the active ingredient can be suitably determined depending on the purpose of use.
본 발명의 구취제거용 의약외품 조성물은 그 제형에 있어서 특별히 한정되는 바가 없으며, 구취제거 효과가 있는 당업계에 공지된 의약외품의 형태로 다양하게 제형화될 수 있다.The quasi-drug composition for removing bad breath of the present invention is not particularly limited in its dosage form, and may be formulated in various forms in the form of quasi-drugs known in the art for removing bad breath.
또한, 각 제형에 있어서 구취 예방, 개선 또는 제거용 의약외품 조성물은 다른 성분들을 기타 의약외품의 제형 또는 사용목적 등에 따라 임의로 선정하여 배합할 수 있다. 예를 들어, 점증제, 안정화제, 용해화제, 비타민, 안료 및 향료와 같은 통상적인 보조제, 및 담체 등을 포함할 수 있다. 본 발명의 봉독 및 벌꿀의 혼합물을 유효성분으로 포함하는 의약외품용 조성물은 그 자체가 천연에서 유래한 것으로 독성 및 부작용이 거의 없어 의약외품 재료로서 안전하게 사용될 수 있다.In addition, in each formulation, the quasi-drug composition for preventing, improving or removing bad breath may be combined with other ingredients selected arbitrarily according to the formulation or purpose of use of other quasi-drugs. For example, conventional adjuvants such as thickeners, stabilizers, solubilizers, vitamins, pigments and flavors, and carriers may be included. The quasi-drug composition containing the mixture of bee venom and honey as an active ingredient of the present invention is derived from nature itself and has little toxicity and side effects, so it can be safely used as a quasi-drug material.
본 발명의 구강용 의약외품 조성물은 당업계에서 통상적으로 제조되는 어떠한 제형으로도 제조될 수 있으며, 예를 들어, 치약, 구강세정제, 구강청정제, 껌, 캔디류, 구강스프레이, 구강용 연고제, 구강용 바니쉬, 구강양치액 및 잇몸 마사지 크림 등의 제형을 가질 수 있으나 이에 제한되는 것은 아니다.The quasi-drug composition for oral use of the present invention can be prepared in any formulation commonly prepared in the art, for example, toothpaste, mouthwash, mouthwash, gum, candy, oral spray, oral ointment, and oral varnish. , oral rinse and gum massage cream, but may have formulations such as, but are not limited thereto.
본 발명의 다른 양태에 따르면, 본 발명은 다음 단계를 포함하는, 구취, 충치 또는 치주질환의 예방 또는 개선용 천연 가식성 항균 필름 조성물을 제조하는 방법을 제공한다:According to another aspect of the present invention, the present invention provides a method for preparing a natural edible antibacterial film composition for preventing or improving halitosis, tooth decay or periodontal disease, comprising the following steps:
(a) 필름형성제를 용매에 용해시킨 후 비타민, 증점제 및 습윤제를 첨가하고 교반하여 가식성 필름 조성물 용액을 제조하는 단계; (a) preparing an edible film composition solution by dissolving a film forming agent in a solvent, adding vitamins, a thickener and a wetting agent, and stirring the mixture;
(b) 봉독 및 벌꿀의 혼합물에 유화제를 혼합하여 유화액 슬러리를 제조하는 단계; 및(b) preparing an emulsion slurry by mixing an emulsifier with a mixture of bee venom and honey; and
(c) 상기 (a) 단계의 가식성 필름 조성물 용액과 상기 (b) 단계의 유화액 슬러리를 60℃에서 혼합하는 단계; 및 (c) mixing the edible film composition solution of step (a) and the emulsion slurry of step (b) at 60°C; and
(d) 상기 (c) 혼합물을 캐스팅하여 필름 형태로 성형하여 열풍 건조시키는 단계.(d) casting the mixture of (c), molding it into a film form, and drying it with hot air.
상기 (d) 단계에서 (c) 혼합물을 기재(substrate) 상에 도포한 후, 건조 수단을 이용하여 목적효과를 달성할 수 있을 정도로 건조함으로써 구취, 충치 또는 치주질환의 예방 또는 개선용 구강 붕해 필름을 제조할 수 있는데, 이때, 상기 구강붕해필름은 1 내지 200㎛의 두께로 형성될 수 있다.Oral disintegrating film for preventing or improving halitosis, tooth decay or periodontal disease by applying the mixture in step (d) on a substrate and then drying it to the extent that the desired effect can be achieved using a drying means It can be prepared, at this time, the oral disintegrating film may be formed to a thickness of 1 to 200㎛.
본 발명의 방법은 조성물을 이용하므로, 중복된 내용은 본 명세서의 과도한 복잡성을 피하기 위하여 그 기재를 생략한다.Since the method of the present invention uses a composition, redundant details are omitted to avoid excessive complexity of the present specification.
본 발명에 의한 천연 항균 물질로 만들어진 가식성 항균필름 조성물에 의하면, 인체에 부작용이 전혀 없을 뿐만 아니라, 필름에 포함된 정제봉독 및 벌꿀의 구취 유발 물질 제거 효능 및 구강 질환 유발균에 대한 항균 작용에 의해서, 구강 질환 유발을 억제하며, 장시간 동안 구취제거가 가능하여 소비자가 간편하게 휴대, 사용할 수 있는 새로운 개념의 구강 청결유지 및 충치생성방지 제품으로 사용될 수 있으며, 식품포장용 항균필름 등으로 이용될 수 있다.According to the edible antibacterial film composition made of natural antibacterial substances according to the present invention, there is no side effect on the human body, and the purified bee venom and honey contained in the film are effective in removing odor-causing substances and have antibacterial action against oral disease-causing bacteria. It can be used as a new concept of oral hygiene maintenance and tooth decay prevention product that can be easily carried and used by consumers by suppressing the occurrence of oral diseases and removing bad breath for a long time, and can be used as an antibacterial film for food packaging. .
도 1은 아랫잇못상피세포주(human lower gingiva epithelial cell)에서의 정제봉독 및 벌꿀에 대한 세포생존율 평가 결과를 보여준다.Figure 1 shows the evaluation results of cell viability for purified bee venom and honey in human lower gingiva epithelial cell.
이하, 본 발명을 실시예를 통하여 더욱 상세히 설명하기로 한다. 이들 실시예는 본 발명을 보다 구체적으로 설명하기 위한 것으로서, 본 발명의 범위가 이들 실시예에 한정되는 것은 아니다.Hereinafter, the present invention will be described in more detail through examples. These examples are intended to explain the present invention in more detail, and the scope of the present invention is not limited to these examples.
실시예Example
실시예 1. 정제봉독 및 벌꿀의 구강 질환 유발균에 대한 항균 활성 평가Example 1. Evaluation of antibacterial activity of purified bee venom and honey against oral disease-causing bacteria
본 발명자들은, 정제봉독 및 벌꿀의 구강 질환 유발균에 대한 항균활성을 하기와 같이 측정하였다.The present inventors measured the antibacterial activity of purified bee venom and honey against oral disease-causing bacteria as follows.
정제봉독은 서양종꿀벌(Apis mellifera L.)의 일벌의 독낭으로부터 봉독을 획득한 후, 간이 정제 방법으로 정제하여 멜리틴 함량이 63 ± 3%인 것을 본 실시예에 사용하였다.Purified bee venom was obtained from the venom sac of worker bees of Western species ( Apis mellifera L.), and then purified by a simple purification method, and the melittin content was 63 ± 3%, and was used in this example.
벌꿀은 다양한 밀원의 벌꿀이 가능하나, 아카시아꿀을 본 실시예에 사용하였다.Honey can be honey of various wheat sources, but acacia honey was used in this example.
대상 균주로서 충치 유발균인 스트렙토코커스 뮤탄스(Streptococcus mutans, ATCC 27175), 잇몸 염증 유발균인 스트렙토코커스 상귀스(Streptococcus sanguis, ATCC 10556), 치주질환 유발균인 포르피로모나스 진지발리스(Porphyromonas Gingivalis) 및 구취를 일으키는 구강내 혐기성 세균인 푸소박테륨 누클레아툼(Fusobacterium nucleatum)를 한국 미생물 보존 센터(대전, 한국)로부터 구입하였다. Target strains include Streptococcus mutans (ATCC 27175), a bacterium that causes tooth decay, Streptococcus sanguis (ATCC 10556), a bacterium that causes gum inflammation, and Porphyromonas gingivalis , a bacterium that causes periodontal disease. ) and Fusobacterium nucleatum, an anaerobic oral bacterium causing halitosis, were purchased from the Korea Microorganism Conservation Center (Daejeon, Korea).
S. mutans 균주는 BHI (Brain heart infusion broth, BD, France) 배지를 사용하여 37℃ 호기조건으로 배양하였고; S. sanguis 균주는 5% sheep blood (MB cell, USA)가 첨가된 TSB (Trypticase soy broth, BD, France) 배지를 사용하여 37℃ 호기조건으로 배양하였으며; P. gingivalis 균주 및 F. nucleatum 균주는 10% sheep blood와 0.1% hemin (Sigma, USA)를 첨가한 TSA 배지 CO2 배양기에서 37℃로 배양하였다. S. mutans strain was cultured in aerobic conditions at 37° C. using BHI (Brain heart infusion broth, BD, France) medium; The S. sanguis strain was cultured under aerobic conditions at 37° C. using TSB (Trypticase soy broth, BD, France) medium supplemented with 5% sheep blood (MB cell, USA); P. gingivalis and F. nucleatum strains were cultured at 37°C in a TSA medium CO 2 incubator supplemented with 10% sheep blood and 0.1% hemin (Sigma, USA).
상기 구강 질환 원인균에 대한 항균 활성 효과를 확인하기 위하여, 평판배지확산법을 이용하였다. In order to confirm the antibacterial activity effect on the oral disease causative bacteria, a plate medium diffusion method was used.
간략하게는, S. mutans를 평판배지에 도말 접종하고, 상기 평판배지에 다양한 조성 비율로 조합한 정제봉독과 벌꿀이 집적된 직경 8 mm의 paper disk (Advantec, Japan)를 올린 후 48시간 배양하면서 생성된 투명한 저지환 크기를 측정하였다.Briefly, S. mutans was smeared on a plate medium, and a paper disk (Advantec, Japan) with a diameter of 8 mm in which purified bee venom and honey, combined in various composition ratios, was accumulated was placed on the plate medium, and cultured for 48 hours. The size of the resulting transparent hypoallergenic ring was measured.
그 결과, 하기 표 1에 나타낸 바와 같이, 정제봉독 함량이 높아질수록 항균 효능은 높아지나, 10 ㎍을 초과하는 군, 즉 100 ㎍ 처리 군에서는 쓴맛이 강해지므로 관능평가에서 불리하였다.As a result, as shown in Table 1 below, the higher the purified bee venom content, the higher the antibacterial efficacy, but the sensory evaluation was disadvantageous in the group exceeding 10 μg, that is, in the group treated with 100 μg, because the bitter taste became stronger.
또한, 본 발명자들은 정제봉독과 벌꿀의 조성 비율에 따른 세포독성을 확인하고자, 아랫잇못상피세포주인 YD-38(human lower gingiva epithelial cell, KCLB 60508, 한국세포주은행, 서울)의 정제봉독 및 벌꿀에 대한 세포생존율을 평가하였다. In addition, in order to confirm the cytotoxicity according to the composition ratio of purified bee venom and honey, the inventors tested the purified bee venom and honey of YD-38 (human lower gingiva epithelial cell, KCLB 60508, Korea Cell Line Bank, Seoul), a lower gum epithelial cell line. Cell viability was evaluated.
YD-38세포는 10% fetal bovine serum (Gibco BRL, USA), 1% penicillin/streptomycin (Gibco BRL, USA)이 첨가된 RPMI 1640 (Gibco BRL, USA) 배지에 5% CO2배양기에서 37℃로 배양하였다. YD-38 cells were grown in RPMI 1640 (Gibco BRL, USA) supplemented with 10% fetal bovine serum (Gibco BRL, USA) and 1% penicillin/streptomycin (Gibco BRL, USA) at 37°C in a 5% CO 2 incubator. cultured.
YD-38세포를 96-웰 플레이트에 5 x 103 세포/mL 농도로 분주하여 24시간 배양 후 각각 다양한 농도로 희석한 시료를 처리하였다.YD-38 cells were dispensed in a 96-well plate at a concentration of 5 x 10 3 cells/mL, and after incubation for 24 hours, samples diluted at various concentrations were treated.
24시간 동안 배양 후 배지를 제거한 다음 50㎕ MTT 용액을 첨가하여 3시간 배양하고 DMSO 200 ㎕를 처리한 다음 570 nm의 흡광도 측정하였다. After culturing for 24 hours, the culture medium was removed, 50 μl of MTT solution was added, followed by incubation for 3 hours, treatment with 200 μl of DMSO, and absorbance at 570 nm was measured.
그 결과, 도 1에 나타낸 바와 같이, 봉독 함량이 높아질수록 세포독성을 유발하고 관능 평가에 불리하므로, 항균 효능을 가지면서 관능평가도 좋은 최적 농도는 벌꿀 10mg 당 정제봉독 1 내지 10㎍, 가장 바람직하게는 1㎍으로 함유되는 것이 적합하였다. As a result, as shown in FIG. 1, since higher bee venom content causes cytotoxicity and is disadvantageous in sensory evaluation, the optimal concentration having antibacterial efficacy and good sensory evaluation is 1 to 10 μg of purified bee venom per 10 mg of honey, most preferably. Preferably, it was suitable to contain 1 μg.
또한, 본 발명자들은 정제봉독 및 벌꿀의 S. mutans, S. sanguis, P. gingivalis 및 F. nucleatum에 대한 항균 효과를 확인하고자, 액체배지 희석법을 사용하여 최소성장억제농도 (Minimun Inhibitory Concentration, MIC)를 측정하였다.In addition, in order to confirm the antibacterial effect of purified bee venom and honey on S. mutans, S. sanguis, P. gingivalis and F. nucleatum , the present inventors used a liquid medium dilution method to obtain a minimum inhibitory concentration (MIC) was measured.
정제봉독은 멸균 증류수로 희석한 후 무균 여과하여 액체배지희석법에 준하여 단계적으로 희석하였다. The purified bee venom was diluted with sterile distilled water, filtered aseptically, and gradually diluted according to the liquid medium dilution method.
각각의 시험균주를 액체배지에서 전-배양한 후 접종균의 2 x 106 CFU/well이 되도록 조절하여 상기 시료와 함께 18시간 동안 배양한 후, 육안 및 현미경으로 균의 성장을 관찰하였다. 이때, 흡수파장 540nm에서 흡광도 (Molecular device spectramax M2e, CA)를 측정하여 순수배양액의 흡광도 값과 같은 결과를 얻은 것을 최소억제농도로 결정하였다.Each test strain was pre-cultured in a liquid medium, adjusted to 2 x 10 6 CFU/well of the inoculum, and incubated with the sample for 18 hours, and then the growth of the strain was observed with the naked eye and under a microscope. At this time, the absorbance (Molecular device spectramax M2e, CA) was measured at an absorption wavelength of 540 nm, and the same result as the absorbance value of the pure culture medium was determined as the minimum inhibitory concentration.
그 결과, 하기 표 2에 나타낸 바와 같이, 정제봉독 및 벌꿀은 다양한 구강 질환 유발 균주에 대하여 우수한 항균 활성을 나타내는 것을 확인할 수 있었다.As a result, as shown in Table 2 below, it was confirmed that the purified bee venom and honey exhibited excellent antibacterial activity against various oral disease-causing strains.
실시예 2. 정제봉독 및 벌꿀의 구취 억제 효과 평가Example 2. Evaluation of the inhibitory effect on bad breath of purified bee venom and honey
본 발명자들은, 정제봉독 및 벌꿀의 비율에 따른 구취 억제 효과를 확인하기 위하여, 할로미터(halimeter)를 이용하여 구취 주성분인 메틸머캅탄(Methylmercaptan)에 대한 억제효과를 측정하였다. The present inventors measured the inhibitory effect on methylmercaptan, the main component of bad breath, using a halimeter to confirm the effect of suppressing bad breath according to the ratio of purified bee venom and honey.
메틸머캅탄 표준용액을 0.2 M 인산칼륨 완충용액에 희석 후 할로미터 측정값이 500-600 ppb가 되도록 한 후 그 희석액을 구취 유발원 용액으로 사용하였다. 정제봉독과 벌꿀 시료를 각각 0.2M 인산칼륨완충용액에 녹여 1% 용액으로 사용하였다. 대조군으로는 시험관에 완충용액 500 ㎕와 구취 유발원 용액 500 ㎕를 첨가하였다. 시험관은 36.5℃ 수조에 5분간 배양 후 할로미터로 측정하였다. 각 시험군의 1% 용액에 대한 구취 성분 억제정도로 하였다.After diluting the methyl mercaptan standard solution in 0.2 M potassium phosphate buffer solution to obtain a halitometer measurement value of 500-600 ppb, the diluted solution was used as a halitosis inducing solution. The purified bee venom and honey samples were dissolved in 0.2M potassium phosphate buffer solution, respectively, and used as a 1% solution. As a control, 500 μl of the buffer solution and 500 μl of the halitosis inducer solution were added to the test tube. The test tube was incubated in a 36.5 ° C water bath for 5 minutes and then measured with a halometer. The degree of inhibition of halitosis components for each 1% solution of each test group was determined.
그 결과, 하기 표 3에 나타낸 바와 같이, 구취 억제효능은 벌꿀에서 기인하였다. 그러나 벌꿀 함량이 높아질 경우 봉독과의 희석이 어려워지며 단맛이 너무 강하다는 단점이 있어 100㎎을 넘지 않는 것이 적정하였다. As a result, as shown in Table 3 below, the halitosis inhibitory effect was attributed to honey. However, if the honey content is high, dilution with bee venom becomes difficult and the sweetness is too strong, so it was appropriate not to exceed 100 mg.
실시예 3.Example 3. 정제 봉독 및 벌꿀을 포함하는 천연 가식성 항균 필름의 제조 Preparation of natural edible antibacterial film containing purified bee venom and honey
본 발명자들은 상기 실시예 1 및 2에 기재된 결과들을 기반으로, 정제 봉독 및 벌꿀의 혼합물(벌꿀 10mg 당 정제봉독 1㎍ 함유)을 이용하여 충치 및 구취 제거에 효과적인 천연 가식성 항균 필름을 제조하였다. Based on the results described in Examples 1 and 2, the present inventors prepared a natural edible antibacterial film effective in removing tooth decay and bad breath using a mixture of purified bee venom and honey (containing 1 μg of purified bee venom per 10 mg of honey).
간략하게, 하기 표 4에 나타낸 바와 같은 첨가제 조성 및 함량으로, 필름형성제를 정제수에 녹인 다음 비타민, 증점제 및 습윤제를 넣고 교반하였다. 유화제를 정제봉독 및 벌꿀의 혼합물에 혼합한 조성물을 첨가하여 슬러리를 만든 후 상기 제조한 수용액과 희석하여 60℃ 수조에서 이들을 혼합시켰다.Briefly, with the composition and content of additives as shown in Table 4 below, the film-forming agent was dissolved in purified water, and then vitamins, a thickener and a wetting agent were added and stirred. A slurry was prepared by adding an emulsifier to a mixture of purified bee venom and honey, and then diluted with the prepared aqueous solution and mixed in a water bath at 60°C.
필름형성 조성물은 트레이에 제조한 용액을 얇게 펴서 부은 후 열풍건조시켜 필름으로 제조하였다.The film-forming composition was prepared into a film by spreading and pouring the prepared solution on a tray and then drying it with hot air.
그 결과, 제조예 9 내지 12는 필름 형태로 잘 형성되었다.As a result, Preparation Examples 9 to 12 were well formed in the form of films.
실시예 4.Example 4. 정제 봉독 및 벌꿀을 포함하는 천연 가식성 항균 필름의 관능 평가 Sensory evaluation of natural edible antibacterial film containing purified bee venom and honey
본 발명자들은 상기 실시예 3에서 제조된 구강 붕해 천연 가식성 항균 필름의 식감, 맛과 향, 편리성 및 선호도 등에 대한 관능평가를 실시하였으며, 그 결과를 하기 표 6에 나타내었다.The present inventors performed sensory evaluation on texture, taste and aroma, convenience and preference of the orally disintegrating natural edible antibacterial film prepared in Example 3, and the results are shown in Table 6 below.
관능시험은 일반 소비자 30명을 대상으로 실시하였고, 점수 및 평가기준은 9점 채점법을 이용하였으며, 하기 표 5에 나타내었다.The sensory test was conducted on 30 general consumers, and the score and evaluation criteria used a 9-point scoring method, and are shown in Table 5 below.
상기 표 6에 나타난 바와 같이, 제조예 7 내지 12를 통해 제조된 구취제거용 구강붕해필름의 식감, 맛과 향, 편리성 및 선호도를 비교한 결과, 본 발명의 구취제거용 구강붕해필름의 식감, 맛과 향, 편리성 및 선호도가 우수한 것을 확인할 수 있었다.As shown in Table 6, as a result of comparing the texture, taste and aroma, convenience and preference of the oral disintegrating films for removing bad breath prepared in Preparation Examples 7 to 12, the oral disintegrating film for removing bad breath of the present invention It was confirmed that the texture, taste and aroma, convenience and preference of the were excellent.
이는, 실시예와 같이 제조된 구취제거용 구강붕해필름은 구강내에 부착한 후 용해하여 사용할 수 있으므로, 사용 후 입안을 헹군 액상형 구강청정제를 외부로 뱉어내야 하는 불편함이 있는 종래의 액상형 구강청정제보다 식감이나 편리성이 현저히 증대되어 소비자의 기호도를 충족시킨 것으로 판단된다.This is because the oral disintegrating film for removing bad breath manufactured as in the example can be used by dissolving after being attached to the oral cavity, so that the liquid mouthwash rinsed after use is inconvenient to spit out the mouth. Conventional liquid mouthwash It is judged that the texture and convenience are significantly increased to satisfy the preference of consumers.
실시예 5.Example 5. 정제 봉독 및 벌꿀을 포함하는 천연 가식성 항균 필름의 구취 억제 평가 Evaluation of anti-halitosis inhibition of natural edible antibacterial film containing purified bee venom and honey
본 발명자들은 상기 실시예 3에서 제조된 구강 붕해 천연 가식성 항균 필름의 구취 억제 효과를 평가하였다. The present inventors evaluated the effect of suppressing bad breath of the orally disintegrating natural edible antibacterial film prepared in Example 3.
구취제거용 구강붕해필름의 구취 억제 효과에 대한 실험을 수행하기 위하여, 치아 우식을 가지고 있지 않은 남녀 20명을 선정하여 실험대상으로 하였다. 실험 대상자의 구취 억제 측정은 할로미터 기기를 이용하여 측정하였다.In order to conduct an experiment on the effect of suppressing bad breath of oral disintegrating film for removing bad breath, 20 men and women who did not have dental caries were selected as test subjects. The halitosis inhibition of the test subjects was measured using a halometer device.
상기 구취 억제 효과 실험은 동일한 식사를 한 후 할로미터로 구취를 측정하여 초기의 구취 정도를 결정하였다. 이후, 제조예에 따라 제조된 구취제거용 구강붕해필름을 구강 내에 부착하여 용해되도록 한 후에 다시 할로미터로 구취를 측정하였다.In the halitosis suppression effect experiment, the degree of bad breath was determined by measuring the bad breath with a halometer after eating the same meal. Thereafter, the oral disintegrating film for removing bad breath prepared according to the preparation example was attached to the oral cavity to be dissolved, and then the bad breath was measured again with a halometer.
제조예에 따라 제조된 구취제거용 구강붕해필름을 적용한 후 구취 억제 효과에 대한 결과는 하기의 표 7에 나타낸 바와 같다.After applying the oral disintegrating film for removing bad breath prepared according to Preparation Example, the results on the effect of suppressing bad breath are shown in Table 7 below.
상기 표 7을 참조하면, 제조예 7 및 제조예 12에 따라 제조된 구취제거용 구강붕해필름은 전반적으로 높은 구취억제율을 보였고, 이중, 특히, 제조예 12에 따라 제조된 구취제거용 구강붕해필름은 95%의 구취 억제율을 보였는데, 상기 조성 비율에 의해 구취 억제 효과가 증진된 것으로 판단된다.Referring to Table 7, the oral disintegrating film for removing bad breath prepared according to Preparation Example 7 and Preparation Example 12 showed a high rate of suppression of bad breath in general, and among them, in particular, oral shelf for removing bad breath prepared according to Preparation Example 12. Haefilm showed a bad breath suppression rate of 95%, and it was determined that the bad breath suppression effect was enhanced by the composition ratio.
이상, 본 발명의 바람직한 일 실시예를 설명하였지만, 본 발명이 속하는 기술분야에서 통상의 지식을 가진 자라면 본 발명이 그 기술적 사상이나 필수적인 특징을 변경하지 않고서 다른 구체적인 형태로 실시될 수 있다는 것을 이해할 수 있을 것이다. 그러므로 이상에서 기술한 일 실시예는 모든 면에서 예시적인 것이며 한정적이 아닌 것으로 이해해야만 한다.Although the preferred embodiment of the present invention has been described above, those skilled in the art will understand that the present invention can be implemented in other specific forms without changing the technical spirit or essential features. You will be able to. Therefore, one embodiment described above should be understood as illustrative in all respects and not limiting.
Claims (11)
상기 봉독 및 벌꿀의 혼합물은 벌꿀 10mg 당 정제봉독 1㎍이 함유되고;
상기 봉독 및 벌꿀의 혼합물은 조성물 기준으로 2%중량으로 함유되며;
상기 풀루란은 조성물 기준으로 1%중량으로 함유되며;
상기 카라기난은 조성물 기준으로 1%중량으로 함유되는 것을 특징으로 하는, 구취, 충치 또는 치주질환의 예방 또는 개선용 천연 가식성 항균 필름 조성물.
A mixture of bee venom and honey as active ingredients; And a natural edible antibacterial film composition for preventing or improving bad breath, tooth decay or periodontal disease, comprising pullulan and carrageenan as film forming agents,
The mixture of bee venom and honey contains 1 μg of purified bee venom per 10 mg of honey;
The mixture of bee venom and honey is contained in an amount of 2% by weight based on the composition;
The pullulan is contained in 1% by weight based on the composition;
The carrageenan is a natural edible antibacterial film composition for preventing or improving halitosis, tooth decay or periodontal disease, characterized in that it is contained in an amount of 1% by weight based on the composition.
상기 봉독은 서양종 꿀벌(Apis mellifera L.) 일벌의 독낭으로부터 분리한 정제봉독인 것을 특징으로 하는,
구취, 충치 또는 치주질환의 예방 또는 개선용 천연 가식성 항균 필름 조성물.
According to claim 1,
Characterized in that the bee venom is a purified bee venom isolated from the bee venom of a western bee ( Apis mellifera L.) worker bee,
A natural edible antibacterial film composition for preventing or improving halitosis, tooth decay or periodontal disease.
상기 조성물은 스트렙토코커스 뮤탄스(Streptococcus mutans), 스트렙토코커스 상귀스(Streptococcus sanguis), 포르피로모나스 진지발리스(Porphyromonas Gingivalis) 및 푸소박테륨 누클레아툼(Fusobacterium nucleatum)로 이루어진 군으로부터 선택되는 1 종 이상의 미생물에 대해 항균 활성을 나타내는 것을 특징으로 하는,
구취, 충치 또는 치주질환의 예방 또는 개선용 천연 가식성 항균 필름 조성물.
According to claim 1,
The composition is one selected from the group consisting of Streptococcus mutans , Streptococcus sanguis , Porphyromonas gingivalis , and Fusobacterium nucleatum Characterized in that it exhibits antibacterial activity against the above microorganisms,
A natural edible antibacterial film composition for preventing or improving halitosis, tooth decay or periodontal disease.
상기 천연 가식성 항균 필름 조성물은 용매, 비타민, 증점제, 습윤제, 유화제, pH 조절제, 영양제, 윤활제, 향미제, 현탁제, 보존제 및 계면활성제로 이루어진 군으로부터 선택되는 1 종 이상을 추가로 포함하는 것을 특징으로 하는,
구취, 충치 또는 치주질환의 예방 또는 개선용 천연 가식성 항균 필름 조성물.
According to claim 1,
The natural edible antibacterial film composition further comprises at least one selected from the group consisting of solvents, vitamins, thickeners, wetting agents, emulsifiers, pH adjusting agents, nutrients, lubricants, flavoring agents, suspending agents, preservatives, and surfactants. characterized by,
A natural edible antibacterial film composition for preventing or improving halitosis, tooth decay or periodontal disease.
(a) 필름형성제로서 풀루란 및 카라기난을 용매에 용해시킨 후 비타민, 증점제 및 습윤제를 첨가하고 교반하여 가식성 필름 조성물 용액을 제조하는 단계;
(b) 봉독 및 벌꿀의 혼합물에 유화제를 혼합하여 유화액 슬러리를 제조하는 단계로서,
상기 봉독 및 벌꿀의 혼합물은 벌꿀 10mg 당 정제봉독 1㎍이 함유되고,
(c) 상기 (a) 단계의 가식성 필름 조성물 용액과 상기 (b) 단계의 봉독 및 벌꿀의 혼합물을 포함하는 유화액 슬러리를 60℃ 수조에서 혼합하여 천연 가식성 항균 필름 조성물을 제조하는 단계로서,
상기 봉독 및 벌꿀의 혼합물은 천연 가식성 항균 필름 조성물 기준으로 2%중량으로 함유되고;
상기 풀루란은 천연 가식성 항균 필름 조성물 기준으로 1%중량으로 함유되며;
상기 카라기난은 천연 가식성 항균 필름 조성물 기준으로 1%중량으로 함유되고; 및
(d) 상기 (c) 천연 가식성 항균 필름 조성물을 캐스팅하여 필름 형태로 성형하여 열풍 건조시키는 단계.A method for producing a natural edible antibacterial film for preventing or improving halitosis, tooth decay or periodontal disease, comprising the following steps:
(a) preparing an edible film composition solution by dissolving pullulan and carrageenan as a film forming agent in a solvent, adding vitamins, a thickener and a wetting agent and stirring;
(b) preparing an emulsion slurry by mixing an emulsifier with a mixture of bee venom and honey,
The mixture of bee venom and honey contains 1 μg of purified bee venom per 10 mg of honey,
(c) preparing a natural edible antibacterial film composition by mixing the edible film composition solution of step (a) and the emulsion slurry containing the mixture of bee venom and honey of step (b) in a water bath at 60 ° C,
The mixture of bee venom and honey is contained in an amount of 2% by weight based on the natural edible antibacterial film composition;
The pullulan is contained in an amount of 1% by weight based on the natural edible antibacterial film composition;
The carrageenan is contained in an amount of 1% by weight based on the natural edible antibacterial film composition; and
(d) casting the natural edible antibacterial film composition of (c), molding it into a film form, and drying it with hot air.
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