KR20190094988A - Composition for prevention or treatment of oral disease comprising Sunflower oil - Google Patents

Abstract

The present invention relates to a composition for the prevention or treatment of oral diseases, including sunflower oil. More specifically, the present invention relates to a quasi-drug composition having the effect of preventing or improving dental caries, periodontal diseases, toothache, tooth sensitivity and bad breath. The composition of the present invention is excellent in antimicrobial activity against bacteria inducing periodontal diseases and dental caries and concentration-dependently inhibits PGE2 which is a pain and inflammation marker. In addition, the present invention is excellent in inhibiting gingivitis formation, suppressing tooth sensitivity symptom and removing bad breath, and thus can be effectively utilized as a quasi-drug composition for preventing or improving one or more oral diseases selected from the group consisting of dental caries, periodontal diseases, toothache, tooth sensitivity and bad breath. Also, the composition of the present invention can be used as pharmaceutical compositions and food compositions.

Description

Composition for prevention or treatment of sunflower oil comprising sunflower oil}

The present invention relates to a composition for preventing or treating oral diseases, including sunflower oil, and more particularly, to a quasi-drug composition having the effect of preventing or improving dental caries, periodontal disease, toothache, syring and bad breath. The present invention also relates to a pharmaceutical composition comprising sunflower oil, a food composition, and a composition for oral cavity comprising sunflower oil.

Healthy teeth are an important factor in determining quality of life, which is said to be one of the five folds. Among dental diseases, dental cavities and periodontal disease are high incidences worldwide, and various clinical symptoms such as pain, malignant dysfunction, destruction of periodontal tissue, bad breath and acute infections. It is known to be a major factor causing tooth loss and tooth loss, and the cause of oral disease is increasing due to changes in diet.

It is known that about 600 to 800 or more microorganisms exist in the human oral cavity, and these microorganisms are controlled by enzymes such as lysozyme secreted by saliva. But the oral environment, rich in nutrients and water, is a good condition for microorganisms to grow, and the tongue and dental plaque provide an excellent habitat for microorganisms. Some of these microorganisms are opportunistic bacteria and cause diseases such as dental caries, periodontal disease, and syphilis (dental hypersensitivity).

There is an antibacterial method of inhibiting the proliferation of oral pathogens that cause dental caries, periodontal disease, bad breath and syring while living in plaque, and various kinds of antibacterial agents including antibiotics having bactericidal and bacteriostatic effects on oral pathogens, It has been developed as an inhibitor and treatment for periodontal disease, pulp and root canal infection.

However, since antibiotics can cause the emergence of oral resistant bacteria and superinfection with systemic side effects on our bodies, long-term use is difficult and can be used only as a therapeutic agent. In addition, antibacterial agents used in mouthwashes include Sangquinarine, Listerine, Peroxide, Chlorhexidine, etc., which have unclear effects on bacteria in the oral cavity. Although the therapeutic effect on periodontal disease is less clear and the price is expensive, Listerin has a slight bacteriostatic effect with alcohol as the main ingredient, but it has a temporary effect in the oral cavity, and also has a harmful effect on tissues for long-term use. There are disadvantages that can appear. In addition, pyroxide, which has been recently added for the whitening effect, is toxic to bacteria, but at the same time toxic to human tissues, there is a problem in safety as well as sometimes resistant bacteria to pyroxide appear in bacteria. In addition, chlorhexidine is known to be the best among the formulations known to date to prevent plaque formation and to prevent and treat periodontal disease. However, chlorhexidine causes irritation to tissue, pigmentation and degeneration of tissues, and especially has a strong irritating taste and a bad smell. Not only there is a problem that can be shown, myocerosis can be caused, there is a carcinogenic disadvantage that can not be used for a long time for the purpose of treatment or prevention, such as limited use in pregnant women.

Sunflower oil is semi-dried oil obtained from the seeds of sunflower Helianthus annuus L. (Asteraceae), and fatty acids are mainly composed of saturated acids (palmitic acid, stearic acid, arachic acid) 8.7 ~ 14.2, oleic acid 14.1 ~ 43.1 , Linoleic acid 44.2 ~ 75.4, fluoride 1.5% or less. Pale yellow bitter liquid oil, suitable for edible use.

Under this background, the present inventors conducted studies to derive natural materials that are effective in preventing and improving oral diseases such as dental caries, periodontal disease, toothache, schizophrenia, and bad breath without worrying about such side effects. . As a result, as a natural-derived composition that can be used safely and safely in the body, sunflower oil exhibits antimicrobial effect against dental caries and periodontal disease-inducing bacteria, inhibitory effect of PGE2, inhibitory gingivitis formation, inhibitory effect of avirin and bad breath removal. It has been found and completed the quasi-drug composition, pharmaceutical composition, food composition and composition for oral cavity using the same.

Korean Patent Publication No. 10-2015-0050239

An object of the present invention is to provide a quasi-drug composition for the prevention or improvement of oral diseases comprising sunflower oil as an active ingredient.

It is another object of the present invention to provide a pharmaceutical composition for preventing or treating oral diseases including sunflower oil as an active ingredient.

Still another object of the present invention is to provide a food composition for preventing or improving oral diseases including sunflower oil as an active ingredient.

Still another object of the present invention is to provide a composition for oral cavity containing sunflower oil as an active ingredient.

The present invention solves the problems as described above, and provides a quasi-drug composition for preventing or improving oral diseases comprising sunflower oil as an active ingredient in order to achieve the object of the present invention.

In another aspect of the present invention, there is provided a pharmaceutical composition for preventing or treating oral diseases including sunflower oil as an active ingredient.

In still another aspect of the present invention, there is provided a food composition for preventing or improving oral diseases including sunflower oil as an active ingredient.

In still another aspect of the present invention, there is provided a composition for oral cavity containing sunflower oil as an active ingredient.

The present invention provides a quasi-drug composition comprising sunflower oil as an active ingredient.

In the present invention, “Sunflower oil” is a semi-dry oil obtained from the seeds of sunflower Helianthus annuus L. (Asteraceae), and fatty acids are saturated acids (palmite, stearic acid, arachic acid) as the main component. 8.7 to 14.2 , Oleic acid 14.1 ~ 43.1, linoleic acid 44.2 ~ 75.4, fluoride 1.5% or less.

Sunflower seeds are known to be good for cardiovascular disease, stabilize cholesterol levels and improve diabetes by controlling blood sugar.

The present invention is not particularly limited to a method of obtaining sunflower oil, and chemically synthesized by a method known in the art, or a commercially available material may be used.

Extraction method of sunflower oil is extracted using chemical solvent, generally using hexane, bonding using milking machine (except at low temperature to prevent oil from being damaged by heat) and using centrifuge In the case of large-scale production, a combination of chemical solvent, milking machine and centrifuge is used.

The present invention provides a quasi-drug composition for oral disease prevention or improvement comprising the sunflower oil as an active ingredient.

In particular, the present invention provides a quasi-drug composition for preventing or ameliorating at least one oral disease selected from the group consisting of dental caries, periodontal disease (periodonitis or gingivitis), toothache, syringe and bad breath comprising the sunflower oil as an active ingredient. Can provide.

In the present invention, "oral disease" refers to various diseases occurring in the oral cavity, and the oral cavity refers to a space in the mouth connected to the pharynx from the anterior lip to the posterior bulb. In the present invention, if the oral disease is a disease occurring in the oral cavity is a concept including all irrespective of the condition, non-limiting examples of the oral disease is dental caries, periodontal disease (periodonitis or gingivitis), toothache, ache, bad breath Etc. can be mentioned.

In the present invention, "dental cavities" is an infectious disease caused by bacteria inhabiting the tooth surface, also called tooth decay, and shows signs of deterioration due to erosion of the hard tissue of the tooth. Specifically, the milky, white, translucent and hard substance covering the surface of the head of the tooth and protecting the dentin of teeth is called tooth enamel or enamel. It is caused by acid caused by the decomposition of sugar and starch by the oral pathogens in the mouth. Tooth decay of the tooth is damaged and tooth decay occurs. The main cause of dental caries is dental plaque (plaque), which is a bacterial membrane formed on the surface of teeth. Saliva forms a thin, sticky membrane on the teeth, and streptococcus sobrinus, a type of streptococci, on it. Oral microorganisms, such as Streptococcus sobrinus and Streptococcus mutans , attach to form biofilms, creating dental plaques and thickening by Fusobacterium . Lose. The dental caries of the present invention includes all irrespective of the species causing the dental caries, specifically, Streptococcus mutans ( Streptococcus mutans ), Streptococcus sanguinis , Streptococcus sobrinus ( Streptococcus sobrinus ), Streptococcus ratti , Streptococcus criceti , Streptococcus anginosus and one or more selected from the group consisting of lactic acid bacteria, more specifically Streptococcus It can be a mutans.

Streptococcus mutans is a type of streptococcus gram-positive bacteria, also called cavities. The Streptococcus mutans proliferate only on the surface of the tooth, but the induction is not completed until about 30 months of age. Therefore, until this time, mutans bacteria are difficult to grow, as in infancy. If other oral bacteria settle in the mouth because they are not infected with mutans from the adult's mouth during infancy, it is difficult for new mutans to enter the already well-balanced oral ecosystem. Will be lowered. If you are already infected with the causative organism through the mouth of the adult, often brush your teeth and keep your mouth clean to help prevent tooth decay. However, once the mutans bacteria are established in the oral cavity, it is impossible to eradicate the mutans bacteria.

In one embodiment of the present invention, after inoculating the disc containing the sunflower oil in the medium plated Streptococcus mutans and measuring the size of the growth inhibitory ring, in the experimental group containing the sunflower oil growth growth ring diameter is 10.0 Significantly good antimicrobial activity against Streptococcus mutans above mm, confirming that compositions comprising sunflower oil can be used for the prevention or improvement of dental caries (Table 2).

In the present invention, "periodontal disease" is a disease in which the gingival, periodontal ligament and bone tissue supporting the teeth are inflamed, which is also commonly referred to as vertigo, gingivitis and periodontitis depending on the severity of the disease. Divided into It is a relatively light and fast-recovering form of periodontal disease, ie gingivitis, which is limited to gums, or soft tissues. There is a V-shaped gap between the gingiva and the teeth, where the oral pathogens attack the area below the gum line of the sulcus, releasing lipopolysaccharide (LPS), which is an irritant. Inflammation is created, such as swelling of the gums and bleeding, which damages the periodontal ligament and adjacent tissues. As periodontitis progresses, the periodontal ligament and even the alveolar bone are damaged, resulting in loss of teeth. Malnutrition of proteins and vitamins, hormonal disorders such as pregnancy or diabetes, smoking and AIDS can make the disease worse. In addition, plaque and calculus are other causes of periodontal disease. If the periodontal disease of the present invention is a causative agent causing periodontal disease, it is included all irrespective of the type, specifically Actinobacillus actinomycetemcomitans , Porphyromonas gingivalis , Tanene At least one homogeneous selected from the group consisting of Tannerella forsythia , Treponema denticola , and Fusobacterium nucleatum , and more specifically, porphyromonas sediment It may be a ballis.

The Pyromonas jinjivalis is a bacteroid (Bacteroide) flexible bacteria, gram-negative bacteria, anaerobic bacteria. The porphyromonas gingivalis is found in the oral cavity in which periodontal disease has occurred, as well as in the upper gastrointestinal tract, respiratory tract and colon. In patients with chronic periodontal disease, collagen is degraded by the bacteria's collagenase enzyme, which can invade gingival fibroblasts and survive under significant concentrations of antibiotics. In addition, the fungus can invade gingival epithelial cells to survive for a long time.

In one embodiment of the present invention, after inoculating the disk containing the sunflower oil in the medium plated porphyromonas gingivalis, and measured the size of the growth inhibitory ring, in the case of the experimental group including the sunflower oil growth growth ring diameter Exceeding the excellent antimicrobial activity against Porphyromonas gingivalis with more than 10.0 mm, it was confirmed that the composition containing sunflower oil can be used for preventing or improving periodontal disease (Table 2).

In addition, in one embodiment of the present invention, as a result of conducting a clinical experiment by selecting the test subjects with gingivitis, the experimental group using the experimental group toothpaste containing the sunflower oil is excellent in gingivitis inhibitory effect and 6 months have passed Although the bleeding effect was maintained by maintaining a normal bleeding state, it was confirmed that the composition containing sunflower oil can be used for preventing or improving periodontal disease including gingivitis (Table 4).

In the present invention, the “toothache” refers to pain in teeth when eating sweet foods or very cold or hot foods. Generally, not only the pain of the teeth itself, but also the periodontal tissue supporting the teeth in the jawbone Pain is also included. Pain usually occurs when chewing, swelling gums, and secretive smell. Toothache is a slightly different pain depending on the causative disease.In particular, the pain caused by dental caries is initially painless, but gradually progresses to deep nerve decay to the nerves in the tooth. Pain can be triggered, and if a tooth breaks or cracks, it can cause pain by stimulating the nerves as the teeth crack when touching or biting cold food. In case of pulpitis, pain is initially felt when cold food is touched, and when it is further developed, pain is felt in hot food, and when inflammation progresses and pulp tissue dies, there is no reaction to cold or hot root tooth (tip of tooth root) Pain caused by inflammation.

In one embodiment of the present invention, as a result of confirming the inhibitory effect of PGE2 known as a pain and inflammation marker, the experimental group including sunflower oil exhibits the effect of inhibiting PGE2 in a concentration-dependent manner, the composition containing sunflower oil is to prevent toothache or It was confirmed that it can be used for improvement purposes (FIG. 1).

In the present invention, "silin" refers to a hypersensitive dentine, and a symptom refers to dentin hyperesthesia. Sirin's symptoms are sensitive when the dentin of exposed teeth comes in contact with cold air or irritating foods, and is present in 60 to 98% of adults with periodontal disease. This symptom is caused by a tooth decay on the gum side due to incorrect brushing habits, excessive occlusal force, or acid dissolution, poor oral hygiene conditions, periodontal treatment, restorative treatment, and acidification of the tooth. May appear. As a fundamental cause of the symptoms, many of the tubules present in the dentin of the tooth are exposed to the outside. By exposing all the stimuli to the nerves in the pulp, they are more sensitive to the same stimulus and can cause pain. have. Syringe symptoms can range from mild to intense, painful pain, and because of the nature of the teeth do not regenerate, painkillers and anti-inflammatory drugs are not a fundamental solution to the symptoms. Shirini symptoms can be seen throughout the entire tooth and may be confined to a specific area, such as the maxilla or mandible, or right or left. The most common areas are different depending on the cause, but mainly the fangs and small molar areas, the most severe pain area is more than 90% of the cervical area, the boundary between the gum and teeth.

In one embodiment of the present invention, as a result of performing a clinical test by selecting a test subject having dentin hypersensitivity teeth, in the experimental group using the experimental group toothpaste containing the sunflower oil has a symptom-suppressing effect excellent, sunflower oil It was confirmed that the composition comprising the sirin can be used for prevention or improvement (Table 6).

In the present invention, "oral breath" is an odor derived from the oral cavity and adjacent organs, and 85 to 90% of the bad breath is derived from the oral cavity, particularly from the back of the tongue. The main components of bad breath are volatile sulfur compounds, of which 90% of the total amount of volatile sulfur compounds is hydrogen sulfide made from cysteine and methyl mercaptan and dimethyl sulfide made from methionine. These components are mainly produced by protein enzymes secreted by anaerobic bacteria, and the back of the tongue is the most important habitat. This area is not well cleaned by saliva, and there are many small depressions, which is a place where bacteria continue to live. The generation of volatile sulfur compounds by anaerobic bacteria is the most important cause of bad breath, but is also caused by oral diseases such as dental caries, periodontitis, dry mouth. Many types of anaerobic bacteria are involved in the development of bad breath, and non-limiting examples of the cause of bad breath include Porphyromonas gingivalis , which secrete many types and large amounts of enzymes.

In one embodiment of the present invention, as a result of performing a clinical experiment on the test subjects without dental caries, the experimental group using the experimental group toothpaste containing the sunflower oil is excellent in bad breath removal effect, the composition comprising sunflower oil It was confirmed that can be used for the prevention or improvement of bad breath (Table 7).

The quasi-drug composition of the present invention may include an oral quasi-drug. Ingredients included in the quasi-drug composition of the present invention may include components commonly used in the quasi-drug composition for oral cavity as an active ingredient, for example, abrasives, wetting agents, binders, foaming agents, sweeteners, preservatives, medicinal ingredients, flavors Agents, dyes, solvents, brighteners, solubilizers or pH adjusters.

Oral quasi-drug composition of the present invention can be prepared in any formulation commonly prepared in the art, for example, toothpaste, mouthwash, mouthwash, gum, candy, oral spray, oral ointment, oral varnish It may have a formulation such as mouthwash, gum massage cream, but is not limited thereto.

As an example, when the oral quasi-drug composition of the present invention is a toothpaste formulation, it may include a humectant, an abrasive, a binder, a foaming agent, a flavoring agent, a sweetening agent, a coloring agent, a preservative, an active ingredient, a solvent, a pH adjusting agent, and the like.

The wetting agent is a powder of the dentifrice component to prevent the toothpaste from being hardened in the air and glycerin, sorbet solution, propylene glycol, polyethylene glycol, etc. by mixing one or two or more of the total weight of the composition 1 to 60 weight %, Specifically 10 to 50% by weight can be used.

The foaming agent enhances the cleaning effect by dispersing the toothpaste in the oral cavity, and acts as a surfactant to clean the oral contamination. Surfactants such as sodium lauryl sulfate, sodium lauryl sodium succinate, sodium alkyl sulfovacate, and sucrose fatty acid ester are used alone. Or by mixing two or more kinds of 0.5 to 10% by weight, specifically 0.5 to 5% by weight of the total weight of the composition can be used.

The binder is to prevent separation between the powder and the liquid component in the toothpaste, cellulose derivatives such as carboxymethyl cellulose sodium, methyl cellulose, hydroxy propyl cellulose and sodium alginate, carrageenan, xanthan gum, etc. alone or in combination of two or more 0.1 to 5% by weight, specifically 0.3 to 2% by weight of the total weight of the composition can be used.

The abrasive is to remove the adhesion of the tooth surface without hurting the surface of the tooth and to polish the original tooth Calcium carbonate (CaCO ₃ ), dicalcium phosphate (CaHPO ₄ , CaHPO ₄ 2H ₂ O), silicic anhydride (SiO ₂ 2H ₂ O), aluminum hydroxide (Al (OH) ₃ ), carium pyrophosphate, magnesium carbonate, or the like, alone or in combination of two or more, 1 to 60% by weight of the total weight of the composition, specifically 10 to 50% by weight Can be used.

The flavoring agent is to enhance the feeling of use by providing a refreshing and odor to the toothpaste, peppermint oil, spearmint oil, menthol, etc. alone or in combination of two or more of 1 to 60% by weight of the total weight of the composition, specifically 0.01 ~ 5% by weight can be used.

The sweetener is to remove the unpleasant taste caused by the raw material of the dentifrice and to improve the refreshing feeling, 1 to 60% by weight of the total weight of the composition alone, or by mixing two or more types of saccharic acid, aspartame, xylitol, licoriceic acid, and the like. 0.01 to 5% by weight can be used.

The active ingredient is for prevention of dental caries, prevention of periodontal disease, prevention of toothache, prevention of syringes and elimination of bad breath, fluoride, zinc chloride, chlorhexidine, aminocapronic acid, tranexamic acid, cetylpyrididium chloride, pyridoxine, Triclosan, tocopherol acetate, sodium monofluorophosphate, etc. can be used individually or in mixture of 2 or more types. In the present invention, sunflower oil can be used as an additional active ingredient.

The quasi-drug composition of the present invention can be used alone or in duplicate, or can be used in combination with other quasi-drug compositions other than the present invention.

As another aspect of the present invention, there is provided a pharmaceutical composition comprising the sunflower oil as an active ingredient. In particular, the present invention is a pharmaceutical composition for preventing or treating at least one oral disease selected from the group consisting of dental caries, periodontal disease (periodonitis or gingivitis), toothache, syringe and bad breath comprising the sunflower oil as an active ingredient. Can be provided. Sunflower oil, oral disease, dental caries, periodontal disease, toothache, syringe and bad breath are as described above.

The pharmaceutical composition of the present invention in the form of tablets, pills, powders, granules, capsules, suspensions, solvents, emulsions, syrups, aerosols, sterile injectable solutions and the like according to conventional methods for preventing and treating oral diseases Formulation is possible.

Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and these solid preparations are prepared by mixing at least one excipient, for example, starch, calcium carbonate, sucrose, lactose, gelatin, and the like. Can be. In addition to the simple excipients, lubricants such as magnesium stearate, talc can also be used. Liquid preparations for oral administration include suspensions, solvents, emulsions, and syrups, and various excipients, for example, wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin. Can be used.

Formulations for parenteral administration may include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized formulations, suppositories, and the like. As the non-aqueous solvent and the suspension solvent, vegetable oils such as propylene glycol, polyethylene glycol, olive oil, etc., injectable esters such as ethyl oleate, and the like can be used.

In addition, the pharmaceutical compositions of the present invention may further comprise a carrier, excipient or diluent. Carriers, excipients or diluents include lactose, textose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, hydroxy Mineral oils such as propyl methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methyl hydroxybenzoate, flophyl hydroxy benzoate, propyl hydroxy benzoate, talc, magnesium stearate, silicon dioxide and the like can be used. have.

The specific dosage of the pharmaceutical composition according to the present invention depends on factors such as the formulation method, the condition and weight of the patient, the sex of the patient, the age, the extent of the disease, the form of the drug, the route and duration of the administration, the rate of excretion, the sensitivity of the reaction, and the like. It can be variously selected by those skilled in the art, and the dosage and frequency do not limit the scope of the present invention in any aspect.

The pharmaceutical composition of the present invention can be administered to mammals such as rats, mice, livestock, humans, etc. through various routes. All modes of administration can be expected and can be administered, for example, by oral, intravenous, intramuscular or subcutaneous injection.

As another aspect of the present invention, the present invention provides a food composition comprising the sunflower oil as an active ingredient. In particular, the present invention provides a food composition for preventing or ameliorating at least one oral disease selected from the group consisting of dental caries, periodontal disease (periodonitis or gingivitis), toothache, syringe and bad breath comprising the sunflower oil as an active ingredient. Can provide. Sunflower oil, oral disease, dental caries, periodontal disease, toothache, syringe and bad breath are as described above. The food composition may be used in the form of health functional food, but is not limited thereto.

The sunflower oil or a food-acceptable salt thereof contained in the food composition of the present invention may be included in the form of a plant, an extract thereof, a fraction thereof, or a processed product thereof. In addition, the composition may contain a food supplement acceptable food additives in addition to the active ingredient.

In the present invention, the "food supplement" means a component that can be added to food supplements, and can be appropriately selected and used by those skilled in the art as being added to prepare the health functional food of each formulation. Examples of food additives include flavors such as various nutrients, vitamins, minerals (electrolytes), synthetic and natural flavors, colorants and fillers, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners. , pH regulators, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like, but is not limited to the kind of food additives of the present invention by the above examples.

The food composition of the present invention may include a health functional food. In the present invention, the "health functional food" refers to a food prepared and processed in the form of tablets, capsules, powders, granules, liquids and pills using raw materials or ingredients having useful functions for the human body. Here, the term "functionality" means obtaining useful effects on health purposes such as nutrient control or physiological effects on the structure and function of the human body. The health functional food of the present invention can be prepared by a method commonly used in the art, and the preparation can be prepared by adding raw materials and ingredients commonly added in the art. In addition, the formulation of the health functional food can also be prepared without limitation as long as the formulation is recognized as a health functional food. Food composition of the present invention can be prepared in various forms of formulation, unlike the general medicine has the advantage that there is no side effect that can occur when taking a long-term use of the drug as a food raw material, excellent portability, Health functional foods can be taken as a supplement to prevent or improve oral disease.

There is no restriction on the form that the health functional food of the present invention can take, and may include all foods in a general sense, and may be mixed with terms known in the art such as functional foods. In addition, the health functional food of the present invention may be prepared by mixing known additives with other appropriate auxiliary ingredients that may be included in the food according to the choice of those skilled in the art. Examples of foods that can be added include meat, sausages, breads, chocolates, candy, snacks, confectionery, pizza, ramen, other noodles, gums, dairy products, including ice cream, various soups, beverages, teas, drinks, alcoholic beverages and Vitamin complexes, and the like, can be prepared by adding to the juice, tea, jelly and juice prepared with the sunflower oil according to the present invention as a main component. It also includes foods used as feed for animals.

The quasi-drug composition comprising sunflower oil of the present invention as an active ingredient is excellent in preventing or improving oral disease. Specifically, the composition of the present invention is excellent in antimicrobial activity against dental caries and periodontal disease-inducing bacteria, concentration-dependent inhibition of pain and inflammation markers, inhibition of gingivitis formation, symptom suppression effect and bad breath removal effect Excellent in utilization as a quasi-drug composition for the prevention or improvement of one or more oral diseases selected from the group consisting of dental caries, periodontal disease, toothache, syringe and bad breath. In addition, the composition containing the sunflower oil of the present invention can be used as a pharmaceutical composition and a food composition.

The following drawings, which are attached to this specification, illustrate preferred embodiments of the present invention, and together with the contents of the present invention serve to further understand the technical spirit of the present invention, the present invention is limited to the matters described in such drawings. It should not be construed as limited.
Figure 1 shows the results of confirming the effect of inhibiting PGE2 by treating LPS (control group), LPS and sunflower oil (experimental group) as inflammatory stimuli to macrophages, respectively.

Hereinafter, the configuration and effects of the present invention through the embodiments will be described in more detail. These examples are only for illustrating the present invention, but the scope of the present invention is not limited by these examples.

Example: sunflower oil

Sunflower oil was used by purchasing sunflower oil sold by Sigma aldrich.

Experimental Example 1: Antibacterial effect on dental caries and periodontitis causing bacteria

Antimicrobial activity experiment was conducted to confirm the preventive or therapeutic effect of the sunflower oil on dental caries and periodontitis. To determine the effect of inhibiting the growth of oral pathogens, the antimicrobial activity test was performed by using the paper disk test using Streptococcus mutans , the representative bacteria of dental caries, and Porphyromonas gingivalis , the representative bacteria of periodontal disease. Was carried out.

After increasing the activity of the oral pathogens in the optimum culture conditions shown in Table 1 below, the culture medium was incubated for 4 to 6 hours in the optimum medium of each bacterium to increase the turbidity of the culture medium. 0.5 (1.5 × 10 ⁸ ) was adjusted and 0.1 ml of each oral pathogen was evenly spread on a plate medium. Thereafter, the sterile paper disk was inoculated with the sunflower oil at a concentration of 10 mg / disc and absorbed and dried for 1 hour. Then, after culturing for 24 to 48 hours at the optimum temperature of each oral pathogen, the size (diameter mm) of growth-lowering ring was measured, and the results are shown in Table 2 below.

Strain Optimal condition Gram Dyeing
Gram staining Strain name Temperature badge characteristics Gram (+) Streptococcus mutans 37 ℃ BHI Facultative anaerobic Gram (-) Porphyromonas gingivalis 37 ℃ TSA Hemin Menadione Badge Anaerobic

Results of Determination of Antimicrobial Activity against Oral Pathogens of Sunflower Oil Growth ring ring diameter (mm) Streptococcus mutans Porphyromonas gingivalis No treatment -(Growth not blocked) -(Growth not blocked) Sunflower oil treatment 10.6 10.3

As shown in Table 2 above, compared to the untreated group not treated with sunflower oil, the group treated with sunflower oil had Streptococcus mutans having a growth-lowering ring diameter of about 10.0 mm for the two oral pathogens. And fairly good antimicrobial activity against Popiromonas gingivalis. Therefore, it was found that sunflower oil can be used for preventing or treating dental caries or periodontal disease.

Experimental Example 2 Inhibitory Effect of Gingivitis Formation

In order to confirm the gingivitis prevention or treatment effect of the sunflower oil, a clinical experiment was performed by selecting a test subject group.

First, sodium carboxymethylcellulose, sodium lauryl sulfate, glycerin, colloidal silicon dioxide, silicas, sodium cocoyl isocyanate, dodysine and sweeteners, fragrances, colorants, etc., which are generally used in toothpaste preparation A control toothpaste was prepared, and the experimental toothpaste was prepared by containing 0.01 wt% of the sunflower oil in the control toothpaste.

In order to screen the subjects, 30 oral patients were examined by age 30 years from age 30 to age 50 in gingivitis patients with even teeth and no missing teeth. After screening, 60 patients were divided into clinical trials to treat gingival inflammation.

Specifically, the test subjects were divided into a control group and a test group, and the control group was trained to use the prepared control toothpaste three times a day before going to sleep 2 hours after eating, and the experimental group used the prepared test toothpaste three times a day at the same time. To be educated. After the periodontal smearing was performed to score the initial gingivitis index, and the control group to use the prepared control toothpaste, the experimental group to use the prepared experimental toothpaste 1 week, 1 month, 3 months and 6 months after oral examination The gingivitis index was checked. The gingivitis index is measured by inserting a periodontal probe into the gingival bulb and burning the probe around each tooth without applying force and measuring the bleeding state after 30 seconds. Scores were recorded according to the results shown in Table 4.

Based on gingivitis index score Contents 0 points Bleeding 1 point Normal bleeding state 2 points Glandular bleeding 3 points Interdental triangle bleeding 4 points Total gingival bleeding

Gingivitis inhibitory effect time Control Experimental group Week 0 1.05 1.04 1 week 1.62 1.06 1 month 2.72 1.08 3 months 2.81 1.12 6 months 2.90 1.13

As shown in Table 4, when compared with the control group, the experimental group using the toothpaste containing sunflower oil maintained the normal bleeding state even after 6 months, the gingival inflammation inhibitory effect continued. Therefore, it was found that sunflower oil can be used for the purpose of preventing or treating gingivitis.

Experimental Example 3 Inhibitory Effect of PGE2, a Pain and Inflammation Marker

In order to confirm the effect of preventing or treating toothache of sunflower oil, the inhibitory effect of PGE2, known as a pain and inflammation marker, was confirmed.

First, macrophages were inoculated at a concentration of 1.5 X 10 ⁵ cells / ml in DMEM medium containing 10% FBS and incubated in a 24 well plate at 37 ° C. and 5% CO ₂ for 24 hours. Then, after incubation for 24 hours with LPS 1 ㎍ / ml and sunflower oil by the concentration of inflammation-induced stimulation factor, PGE2 ELISA assay kit (Thermo SCIENTIFIC) was purchased and supernatant was used to inhibit PGE2 inhibition according to sunflower oil concentration. Analyzes (FIG. 1).

As shown in Figure 1, compared to the group treated with LPS only, 50ppm sunflower oil up to about 25%, 100ppm sunflower oil up to about 50% was effective in inhibiting PGE2. Therefore, it was found that sunflower oil can be used for the purpose of preventing or treating toothache.

Experimental Example 4: Effect of Seroin Inhibition

In order to confirm the prevention or treatment effect of the sunflower oil, the experimental subjects were selected and clinical trials were performed. The control toothpaste and the experimental group toothpaste used in the clinical trial were prepared in the same manner as the toothpaste of Experimental Example 2, respectively.

Subjects were 40 volunteers who had dentin hypersensitivity teeth and agreed to participate in the trial, with a total of 80 subjects. In addition, 20 males and 20 females were 20 to 50 years old. The subjects were not aware of the toothpaste contents and the total duration of the experiment was 2 weeks.

The experiment was performed by measuring the response of the subject after applying a temperature stimulus. Before conducting the experiment, check the sensitive areas of the dentin hypersensitivity teeth of each subject in advance, and drop the cold water of about 5 ° C with an eyedropper on the aerobic area of the tooth with a dropper, and then scored according to the criteria shown in Table 5 below. In the control toothpaste, the experimental group was allowed to use the experimental toothpaste three times a day for two weeks, and after two weeks, drop the cold water of about 5 ° C with an eyedropper to score. Statistical treatment was tested before and after the experiment stimulation scores by paired Student-t test (paired Student-t test), the response scores after 2 weeks for temperature stimulation is shown in Table 6 below.

Score criteria for subjects after stimulation score Contents 0 points There is no discomfort. 1 point Slightly uncomfortable or cold 2 points Sour 3 points pain.

Degree of suppression time Control Experimental group Week 0 2.13 ± 0.12 2.15 ± 0.14 2 weeks 2.14 ± 0.19 1.81 ± 0.11 ^* p> 0.05 * p <0.05

※ p> 0.05: 95% confidence, no statistically significant difference. * p <0.05: 95% confidence, statistically significant difference.

As shown in Table 6, when compared with the control group, the experimental group using the toothpaste containing sunflower oil was suppressed after two weeks. Therefore, it was found that sunflower oil can be used for the purpose of preventing or treating syringes.

Experimental Example 5: Bad breath removal effect

In order to confirm the effect of preventing or treating the bad breath of sunflower oil, the test subjects were selected and clinical trials were performed. The control toothpaste and the experimental group toothpaste used in the clinical trial were prepared in the same manner as the toothpaste of Experimental Example 2, respectively.

As a test subject, 50 men and women without dental caries were selected and the cross-over test was performed on the control toothpaste and the test group toothpaste. Commercial garlic powder was dispersed in water, left to stand for 24 hours, and diluted to make a Halimeter measurement value of 700 ppb or more, and the diluent was used as a source of bad breath. The subjects were gargled with 15 ml of garlic powder for 30 seconds, and after 1 minute, the degree of bad breath was measured with a halimeter, and the control group used the control toothpaste, and the experimental group used the test group toothpaste, respectively, for 30 seconds to 1 minute. . After 1 minute, 5 minutes, and 30 minutes after brushing the teeth, the degree of bad breath was measured by measuring the severity of the bad breath.

Bad breath removal effect time Control Experimental group 1 minute 56.8% 95.2% 5 minutes 48.1% 89.2% 30 minutes 39.2% 84.1%

As shown in Table 7, when compared with the control group, the experimental group using the toothpaste containing sunflower oil was removed bad breath about 95% after brushing, even after 30 minutes after brushing the bad breath removal rate of 84% or more removed The effect was much better than the control. Therefore, it was found that sunflower oil can be used for the purpose of preventing or treating bad breath.

From the above description, those skilled in the art will appreciate that the present invention can be implemented in other specific forms without changing the technical spirit or essential features. In this regard, the embodiments described above are to be understood in all respects as illustrative and not restrictive. The scope of the present invention should be construed that all changes or modifications derived from the meaning and scope of the following claims and equivalent concepts rather than the detailed description are included in the scope of the present invention.

Claims (14)

Oral care composition for preventing or improving oral disease comprising sunflower oil as an active ingredient.
According to claim 1, wherein the oral disease is dental caries, quasi-drug composition.
The quasi-drug composition according to claim 2, wherein the dental caries is caused by Streptococcus mutans .
According to claim 1, wherein the oral disease is periodontal disease, quasi-drug composition.
The quasi-drug composition according to claim 4, wherein the periodontal disease is periodontitis or gingivitis.
The quasi-drug composition of claim 4, wherein the periodontal disease is caused by Porphyromonas gingivalis .
According to claim 1, wherein the oral disease is toothache, quasi-drug composition.
The quasi-drug composition according to claim 1, wherein the oral disease is syringy.
The quasi-drug composition according to claim 1, wherein the oral disease is bad breath.
The quasi-drug composition according to claim 1, wherein the quasi-drug is for oral use.
The method of claim 10, wherein the dosage form of the quasi-drug composition is one or more formulations selected from the group consisting of toothpaste, mouthwash, mouthwash, gum, candy, oral spray, oral ointment, oral varnish, oral toothpaste and gum massage cream. It will be quasi-drug composition.
Pharmaceutical composition for preventing or treating oral diseases comprising sunflower oil as an active ingredient.
Food composition for preventing or improving oral diseases, including sunflower oil as an active ingredient.
Oral composition comprising sunflower oil as an active ingredient.

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