KR20190094987A - Composition for prevention or treatment of oral disease comprising Ginkgo biloba extract - Google Patents

Abstract

The present invention relates to a composition for the prevention or treatment of oral diseases, containing a Ginkgo biloba extract. More specifically, the present invention relates to a quasi-drug composition having the effect of preventing or alleviating dental caries, periodontal diseases, toothache, tooth sensitivity and bad breath. The composition of the present invention is excellent in antimicrobial activities against bacteria inducing periodontal diseases and dental caries and concentration-dependently inhibits PGE2 which is a pain and inflammation marker. In addition, the present invention is excellent in inhibiting gingivitis formation, suppressing tooth sensitivity symptom and removing bad breath, and thus can be effectively utilized as a quasi-drug composition for preventing or alleviating one or more oral diseases selected from the group consisting of dental caries, periodontal diseases, toothache, tooth sensitivity and bad breath. Also, the composition of the present invention can be used as pharmaceutical compositions and food compositions.

Description

Composition for prevention or treatment of oral disease comprising Ginkgo biloba extract}

The present invention relates to a composition for preventing or treating oral disease, including ginkgo biloba extract, and more particularly, to a quasi-drug composition having the effect of preventing or improving dental caries, periodontal disease, toothache, syring and bad breath. The present invention also relates to a pharmaceutical composition comprising a ginkgo extract, a food composition and a composition for oral cavity comprising a ginkgo extract.

Healthy teeth are an important factor in determining quality of life, which is said to be one of the five folds. Among dental diseases, dental cavities and periodontal disease are high incidences worldwide, and various clinical symptoms such as pain, malignant dysfunction, destruction of periodontal tissue, bad breath and acute infections. It is known to be a major factor causing tooth loss and tooth loss, and the cause of oral disease is increasing due to changes in diet.

It is known that about 600 to 800 or more microorganisms exist in the human oral cavity, and these microorganisms are controlled by enzymes such as lysozyme secreted by saliva. But the oral environment, rich in nutrients and water, is a good condition for microorganisms to grow, and the tongue and dental plaque provide an excellent habitat for microorganisms. Some of these microorganisms are opportunistic bacteria and cause diseases such as dental caries, periodontal disease, and syphilis (dental hypersensitivity).

There is an antibacterial method of inhibiting the proliferation of oral pathogens that cause dental caries, periodontal disease, bad breath and syring while living in plaque, and various kinds of antibacterial agents including antibiotics having bactericidal and bacteriostatic effects on oral pathogens, It has been developed as an inhibitor and treatment for periodontal disease, pulp and root canal infection.

However, since antibiotics can cause the emergence of oral resistant bacteria and superinfection with systemic side effects on our bodies, long-term use is difficult and can be used only as a therapeutic agent. In addition, antibacterial agents used in mouthwashes include Sangquinarine, Listerine, Peroxide, Chlorhexidine, etc., which have unclear effects on bacteria in the oral cavity. Although the therapeutic effect on periodontal disease is less clear and the price is expensive, Listerin has a slight bacteriostatic effect with alcohol as the main ingredient, but it has a temporary effect in the oral cavity, and also has a harmful effect on tissues for long-term use. There are disadvantages that can appear. In addition, pyroxide, which has been recently added for the whitening effect, is toxic to bacteria, but at the same time toxic to human tissues, there is a problem in safety as well as sometimes resistant bacteria to pyroxide appear in bacteria. In addition, chlorhexidine is known to be the best among the formulations known to date to prevent plaque formation and to prevent and treat periodontal disease. However, chlorhexidine causes irritation to tissue, pigmentation and degeneration of tissues, and especially has a strong irritating taste and a bad smell. Not only there is a problem that can be shown, myocerosis can be caused, there is a carcinogenic disadvantage that can not be used for a long time for the purpose of treatment or prevention, such as limited use in pregnant women.

On the other hand, Ginkgo biloba extract is an ingredient extracted from the fruit of Ginkgo biloba, which belongs to the deciduous tree and is distributed all over the country except high mountains, highlands and low temperatures in the north. Banks contain nutrients such as carotene and vitamin C.

Under this background, the present inventors conducted studies to derive natural materials that are effective in preventing and improving oral diseases such as dental caries, periodontal disease, toothache, schizophrenia, and bad breath without worrying about such side effects. . As a result, ginkgo biloba extract is a natural-derived composition that is safe for use in the body and can be used with confidence, showing antibacterial effect against dental caries and periodontal disease-inducing bacteria, inhibitory effect of PGE2, inhibitory gingivitis formation, inhibitory effect of virin and bad breath removal. It was found to complete the quasi-drug composition, pharmaceutical composition, food composition and composition for oral cavity using the same.

Korean Patent Publication No. 10-2015-0050239

An object of the present invention is to provide a quasi-drug composition for the prevention or improvement of oral diseases comprising a ginkgo extract as an active ingredient.

It is another object of the present invention to provide a pharmaceutical composition for preventing or treating oral diseases including a ginkgo extract as an active ingredient.

Still another object of the present invention is to provide a food composition for preventing or improving oral disease, comprising a ginkgo extract as an active ingredient.

Still another object of the present invention is to provide a composition for oral cavity containing ginkgo extract as an active ingredient.

The present invention solves the above problems, and provides a quasi-drug composition for oral disease prevention or improvement comprising ginkgo extract as an active ingredient in order to achieve the object of the present invention.

In another aspect of the present invention, there is provided a pharmaceutical composition for preventing or treating oral disease, comprising a ginkgo extract as an active ingredient.

In still another aspect of the present invention, there is provided a food composition for preventing or improving oral disease, comprising a ginkgo extract as an active ingredient.

As another aspect of the present invention, there is provided a composition for oral cavity containing the ginkgo extract as an active ingredient.

The present invention provides a quasi-drug composition comprising a ginkgo extract as an active ingredient.

In the present invention, “Ginkgo biloba extract” is an ingredient obtained from the fruit of the ginkgo biloba, and the ginkgo belongs to the deciduous tree, except for high mountains, highlands, and low temperatures in the north. It is distributed throughout the country, and banks contain nutritional ingredients such as carotene and vitamin C.

The present invention is not particularly limited to the method of obtaining ginkgo extract, and chemically synthesized by a method known in the art, or a commercially available material may be used.

As used herein, the term "extract" refers to a liquid component obtained by immersing a desired substance in various solvents and then extracting the mixture for a predetermined time at room temperature, low temperature, or warm state, and removing the solvent from the liquid component. The resulting solid content is meant. In addition, in addition to the result, it can be comprehensively interpreted to include all of the dilution of the resultant, their concentrates, their modifiers, purified products and the like.

In the present invention, the extract means a ginkgo extract. The ginkgo extract can be obtained by extraction with water or various organic solvents. At this time, the organic solvent to be used is not particularly limited as long as it can obtain an extract having an oral disease prevention or treatment effect, specifically, may be water, a polar solvent or a non-polar solvent, more specifically water, Lower alcohols having 1 to 6 carbon atoms (methanol, ethanol, propanol or butanol, etc.), mixed solvents thereof, and most specifically ethanol or mixed solvents thereof.

In addition, the method for obtaining the extract is not particularly limited as long as it can obtain an extract having an oral disease prevention or treatment effect, specifically, the fruit of the ginkgo, leaves, dried products, processed products, etc. Cold extraction method, which is immersed in a solvent and extracted at room temperature, heating extraction method that is extracted by heating to 40 to 100, ultrasonic extraction method by applying ultrasonic waves, reflux extraction method using a reflux condenser, and the like can be used.

The present invention provides a quasi-drug composition for oral disease prevention or improvement comprising the ginkgo extract as an active ingredient.

In addition, the present invention specifically comprises one or more quasi-drug composition for preventing or improving oral disease selected from the group consisting of dental caries, periodontal disease (periodonitis or gingivitis), toothache, syringe and bad breath comprising the ginkgo extract as an active ingredient. Can provide.

In the present invention, "oral disease" refers to various diseases occurring in the oral cavity, and the oral cavity refers to a space in the mouth connected to the pharynx from the anterior lip to the posterior bulb. In the present invention, if the oral disease is a disease occurring in the oral cavity is a concept including all irrespective of the condition, non-limiting examples of the oral disease is dental caries, periodontal disease (periodonitis or gingivitis), toothache, ache, bad breath Etc. can be mentioned.

In the present invention, "dental cavities" is an infectious disease caused by bacteria inhabiting the tooth surface, also called tooth decay, and shows signs of deterioration due to erosion of the hard tissue of the tooth. Specifically, the milky, white, translucent and hard substance covering the surface of the head of the tooth and protecting the dentin of teeth is called tooth enamel or enamel. It is caused by acid caused by the decomposition of sugar and starch by the oral pathogens in the mouth. Tooth decay of the tooth is damaged and tooth decay occurs. The main cause of dental caries is dental plaque (plaque), which is a bacterial membrane formed on the surface of teeth. Saliva forms a thin, sticky membrane on the teeth, and streptococcus sobrinus, a type of streptococci, on it. Oral microorganisms, such as Streptococcus sobrinus and Streptococcus mutans , attach to form biofilms, creating dental plaques and thickening by Fusobacterium . Lose. The dental caries of the present invention include all irrespective of the species causing the dental caries, specifically, Streptococcus mutans ( Streptococcus mutans ), Streptococcus sanguinis , Streptococcus sobrinus ( Streptococcus sobrinus ), Streptococcus ratti , Streptococcus criceti , Streptococcus anginosus and one or more selected from the group consisting of lactic acid bacteria, more specifically Streptococcus It can be a mutans.

Streptococcus mutans is a type of streptococcus gram-positive bacteria, also called cavities. The Streptococcus mutans proliferate only on the surface of the tooth, but the induction is not completed until about 30 months of age. Therefore, until this time, mutans bacteria are difficult to grow, as in infancy. If other oral bacteria settle in the mouth because they are not infected with mutans from the adult's mouth during infancy, it is difficult for new mutans to enter the already well-balanced oral ecosystem. Will be lowered. If you are already infected with the causative organism through the mouth of the adult, often brush your teeth and keep your mouth clean to help prevent tooth decay. However, once the mutans bacteria are established in the oral cavity, it is impossible to eradicate the mutans bacteria.

In one embodiment of the present invention, after inoculating the disk containing the ginkgo extract in the medium plated Streptococcus mutans and measuring the size of the growth inhibitory ring, in the case of the experimental group including the ginkgo extract, Significantly good antibacterial activity against Streptococcus mutans above 11.0 mm, confirming that compositions comprising ginkgo extract can be used for preventing or improving dental caries (Table 2).

In the present invention, "periodontal disease" is a disease in which the gingival, periodontal ligament and bone tissue supporting the teeth are inflamed, which is also commonly referred to as vertigo, gingivitis and periodontitis depending on the severity of the disease. Divided into It is a relatively light and fast-recovering form of periodontal disease that is limited to the gums, ie soft tissues, is called gingivitis, and when this inflammation progresses to the gums and around the gum bone, it is called periodontitis. There is a V-shaped gap between the gingiva and the teeth, where the oral pathogens attack the area below the gum line of the sulcus, releasing lipopolysaccharide (LPS), which is an irritant. Inflammation is created, such as swelling of the gums and bleeding, which damages the periodontal ligament and adjacent tissues. As periodontitis progresses, the periodontal ligament and even the alveolar bone are damaged, resulting in tooth loss. Malnutrition of proteins and vitamins, hormonal disorders such as pregnancy or diabetes, smoking and AIDS can make the disease worse. In addition, plaque and calculus are other causes of periodontal disease. If the periodontal disease of the present invention is a causative agent causing periodontal disease, it is included all irrespective of the type, specifically Actinobacillus actinomycetemcomitans , Porphyromonas gingivalis , Tanene At least one homogeneous selected from the group consisting of Tannerella forsythia , Treponema denticola , and Fusobacterium nucleatum , and more specifically, porphyromonas sediment It may be a ballis.

The Pyromonas jinjivalis is a bacteroid (Bacteroide) flexible bacteria, gram-negative bacteria, anaerobic bacteria. The porphyromonas gingivalis is found in the oral cavity in which periodontal disease has occurred, as well as in the upper gastrointestinal tract, respiratory tract and colon. In patients with chronic periodontal disease, collagen is degraded by the bacteria's collagenase enzyme, which can invade gingival fibroblasts and survive under significant concentrations of antibiotics. In addition, the fungus can invade gingival epithelial cells to survive for a long time.

In one embodiment of the present invention, after inoculating the disk containing the ginkgo biloba extract in the medium plated porphyromonas gingivalis, and measured the size of the growth inhibitory ring, in the case of the experimental group containing the ginkgo extract growth growth ring diameter This 11.0 mm or more exhibits a very good antimicrobial activity against Porphyromonas gingivalis, confirming that the composition comprising the ginkgo extract can be used for the prevention or improvement of periodontal disease (Table 2).

In addition, in one embodiment of the present invention, as a result of performing a clinical experiment by selecting the test subjects with gingivitis, the experimental group using the experimental group toothpaste containing the ginkgo extract is excellent in gingivitis inhibitory effect and 6 months have elapsed Although it was maintained a normal bleeding state to maintain the gingival inflammation inhibitory effect, it was confirmed that the composition containing the ginkgo extract can be used for preventing or improving periodontal disease, including gingivitis (Table 4).

In the present invention, the “toothache” refers to pain in teeth when eating sweet foods or very cold or hot foods. Generally, not only the pain of the teeth itself, but also the periodontal tissue supporting the teeth in the jawbone Pain is also included. Pain usually occurs when chewing, swelling gums, and secretive smell. Toothache is a slightly different pain depending on the causative disease.In particular, the pain caused by dental caries is initially painless, but gradually progresses to deep nerve decay to the nerves in the tooth. Pain can be triggered, and if a tooth breaks or cracks, it can cause pain by stimulating the nerves as the teeth crack when touching or biting cold food. In case of pulpitis, pain is initially felt when cold food is touched, and when it is further developed, pain is felt in hot food, and when inflammation progresses and pulp tissue dies, there is no reaction to cold or hot root tooth (tip of tooth root) Pain caused by inflammation.

In one embodiment of the present invention, as a result of confirming the inhibitory effect of PGE2 known as a pain and inflammation marker, the experimental group including the ginkgo extract exhibits the effect of inhibiting PGE2 in a concentration-dependent manner, the composition comprising the ginkgo extract prevents toothache or It was confirmed that it can be used for improvement purposes (FIG. 1).

In the present invention, "silin" refers to a hypersensitive dentine, and a symptom refers to dentin hyperesthesia. Sirin's symptoms are sensitive when the dentin of exposed teeth comes in contact with cold air or irritating foods, and is present in 60 to 98% of adults with periodontal disease. This symptom is caused by a tooth decay on the gum side due to incorrect brushing habits, excessive occlusal force, or acid dissolution, poor oral hygiene conditions, periodontal treatment, restorative treatment, and acidification of the tooth. May appear. As a fundamental cause of the symptoms, many of the tubules present in the dentin of the tooth are exposed to the outside. By exposing all the stimuli to the nerves in the pulp, they are more sensitive to the same stimulus and can cause pain. have. Syringe symptoms can range from mild to intense, painful pain, and because of the nature of the teeth do not regenerate, painkillers and anti-inflammatory drugs are not a fundamental solution to the symptoms. Shirini symptoms can be seen throughout the entire tooth and may be confined to a specific area, such as the maxilla or mandible, or right or left. The most common areas are different depending on the cause, but mainly the fangs and small molar areas, the most severe pain area is more than 90% of the cervical area, the boundary between the gum and teeth.

In one embodiment of the present invention, as a result of performing a clinical trial by selecting a test subject having dentin hypersensitivity teeth, in the experimental group using the experimental group toothpaste containing the ginkgo extract, the syrup is excellent in symptomatic effect, ginkgo extract It was confirmed that the composition comprising the sirin can be used for prevention or improvement (Table 6).

In the present invention, "oral breath" is an odor derived from the oral cavity and adjacent organs, and 85 to 90% of the bad breath is derived from the oral cavity, particularly from the back of the tongue. The main components of bad breath are volatile sulfur compounds, of which 90% of the total amount of volatile sulfur compounds is hydrogen sulfide made from cysteine and methyl mercaptan and dimethyl sulfide made from methionine. These components are mainly produced by protein enzymes secreted by anaerobic bacteria, and the back of the tongue is the most important habitat. This area is not well cleaned by saliva, and there are many small depressions, which is a place where bacteria continue to live. The generation of volatile sulfur compounds by anaerobic bacteria is the most important cause of bad breath, but is also caused by oral diseases such as dental caries, periodontitis, dry mouth. Many types of anaerobic bacteria are involved in the development of bad breath, and non-limiting examples of the cause of bad breath include Porphyromonas gingivalis , which secrete many types and large amounts of enzymes.

In one embodiment of the present invention, as a result of performing a clinical experiment on the test subjects without dental caries, the experimental group using the experimental group toothpaste containing the ginkgo extract, the effect of removing bad breath, the composition comprising the ginkgo extract It was confirmed that can be used for the prevention or improvement of bad breath (Table 7).

The quasi-drug composition of the present invention may include an oral quasi-drug. Ingredients included in the quasi-drug composition of the present invention may include components commonly used in the quasi-drug composition for oral cavity as an active ingredient, for example, abrasives, wetting agents, binders, foaming agents, sweeteners, preservatives, medicinal ingredients, flavors Agents, dyes, solvents, brighteners, solubilizers or pH adjusters.

Oral quasi-drug composition of the present invention can be prepared in any formulation commonly prepared in the art, for example, toothpaste, mouthwash, mouthwash, gum, candy, oral spray, oral ointment, oral varnish It may have a formulation such as mouthwash, gum massage cream, but is not limited thereto.

As an example, when the oral quasi-drug composition of the present invention is a toothpaste formulation, it may include a humectant, an abrasive, a binder, a foaming agent, a flavoring agent, a sweetening agent, a coloring agent, a preservative, an active ingredient, a solvent, a pH adjusting agent, and the like.

The wetting agent is a powder of the dentifrice component to prevent the toothpaste from being hardened in the air and glycerin, sorbet solution, propylene glycol, polyethylene glycol, etc. by mixing one or two or more of the total weight of the composition 1 to 60 weight %, Specifically 10 to 50% by weight can be used.

The foaming agent enhances the cleaning effect by dispersing the toothpaste in the oral cavity, and acts as a surfactant to clean the oral contamination. Surfactants such as sodium lauryl sulfate, sodium lauryl sodium succinate, sodium alkyl sulfovacate, and sucrose fatty acid ester are used alone. Or by mixing two or more kinds of 0.5 to 10% by weight, specifically 0.5 to 5% by weight of the total weight of the composition can be used.

The binder is to prevent separation between the powder and the liquid component in the toothpaste, cellulose derivatives such as carboxymethyl cellulose sodium, methyl cellulose, hydroxy propyl cellulose and sodium alginate, carrageenan, xanthan gum, etc. alone or in combination of two or more 0.1 to 5% by weight, specifically 0.3 to 2% by weight of the total weight of the composition can be used.

The abrasive is to remove the adhesion of the tooth surface without hurting the surface of the tooth and to polish the original tooth Calcium carbonate (CaCO ₃ ), dicalcium phosphate (CaHPO ₄ , CaHPO ₄ 2H ₂ O), silicic anhydride (SiO ₂ 2H ₂ O), aluminum hydroxide (Al (OH) ₃ ), carium pyrophosphate, magnesium carbonate, or the like, alone or in combination of two or more, 1 to 60% by weight of the total weight of the composition, specifically 10 to 50% by weight Can be used.

The flavoring agent is to enhance the feeling of use by providing a refreshing and odor to the toothpaste, peppermint oil, spearmint oil, menthol, etc. alone or in combination of two or more of 1 to 60% by weight of the total weight of the composition, specifically 0.01 ~ 5% by weight can be used.

The sweetener is to remove the unpleasant taste caused by the raw material of the dentifrice and to improve the refreshing feeling, 1 to 60% by weight of the total weight of the composition alone, or by mixing two or more types of saccharic acid, aspartame, xylitol, licoriceic acid, and the like. 0.01 to 5% by weight can be used.

The active ingredient is for prevention of dental caries, prevention of periodontal disease, prevention of toothache, prevention of syringes and elimination of bad breath, fluoride, zinc chloride, chlorhexidine, aminocapronic acid, tranexamic acid, cetylpyrididium chloride, pyridoxine, Triclosan, tocopherol acetate, sodium monofluorophosphate, etc. can be used individually or in mixture of 2 or more types. In the present invention, ginkgo extract can be used as an additional active ingredient.

The quasi-drug composition of the present invention can be used alone or in duplicate, or can be used in combination with other quasi-drug compositions other than the present invention.

As another aspect of the invention, there is provided a pharmaceutical composition comprising the ginkgo extract as an active ingredient. In particular, the present invention is a pharmaceutical composition for preventing or treating at least one oral disease selected from the group consisting of dental caries, periodontal disease (periodonitis or gingivitis), toothache, syringe and bad breath comprising the ginkgo extract as an active ingredient. Can be provided. Ginkgo biloba extract, oral disease, dental caries, periodontal disease, toothache, syringe and bad breath are as described above.

The pharmaceutical composition of the present invention in the form of tablets, pills, powders, granules, capsules, suspensions, solvents, emulsions, syrups, aerosols, sterile injectable solutions and the like according to conventional methods for preventing and treating oral diseases Formulation is possible.

Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and these solid preparations are prepared by mixing at least one excipient, for example, starch, calcium carbonate, sucrose, lactose, gelatin, and the like. Can be. In addition to the simple excipients, lubricants such as magnesium stearate, talc can also be used. Liquid preparations for oral administration include suspensions, solvents, emulsions, and syrups, and various excipients, for example, wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin. Can be used.

Formulations for parenteral administration may include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized formulations, suppositories, and the like. As the non-aqueous solvent and the suspension solvent, vegetable oils such as propylene glycol, polyethylene glycol, olive oil, etc., injectable esters such as ethyl oleate, and the like can be used.

In addition, the pharmaceutical compositions of the present invention may further comprise a carrier, excipient or diluent. Carriers, excipients or diluents include lactose, textose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, hydroxy Mineral oils such as propyl methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methyl hydroxybenzoate, flophyl hydroxy benzoate, propyl hydroxy benzoate, talc, magnesium stearate, silicon dioxide and the like can be used. have.

The specific dosage of the pharmaceutical composition according to the present invention depends on factors such as the formulation method, the condition and weight of the patient, the sex of the patient, the age, the extent of the disease, the form of the drug, the route and duration of the administration, the rate of excretion, the sensitivity of the reaction, and the like. It can be variously selected by those skilled in the art, and the dosage and frequency do not limit the scope of the present invention in any aspect.

The pharmaceutical composition of the present invention can be administered to mammals such as rats, mice, livestock, humans, etc. through various routes. All modes of administration can be expected and can be administered, for example, by oral, intravenous, intramuscular or subcutaneous injection.

As another aspect of the invention, the present invention provides a food composition comprising the ginkgo extract as an active ingredient. In addition, the present invention specifically comprises at least one oral disease prevention or improvement of food composition selected from the group consisting of dental caries, periodontal disease (periodonitis or gingivitis), toothache, syringe and bad breath comprising the ginkgo extract as an active ingredient Can provide. Ginkgo biloba extract, oral disease, dental caries, periodontal disease, toothache, syringe and bad breath are as described above. The food composition may be used in the form of health functional food, but is not limited thereto.

The ginkgo extract contained in the food composition of the present invention may be included in the form of a plant or animal, extracts thereof, fractions thereof or processed products including the ginkgo extract. In addition, the composition may contain a food supplement acceptable food additives in addition to the active ingredient.

In the present invention, the "food supplement" means a component that can be added to food supplements, and can be appropriately selected and used by those skilled in the art as being added to prepare the health functional food of each formulation. Examples of food additives include flavors such as various nutrients, vitamins, minerals (electrolytes), synthetic and natural flavors, colorants and fillers, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners. , pH regulators, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like, but is not limited to the kind of food additives of the present invention by the above examples.

The food composition of the present invention may include a health functional food. In the present invention, the "health functional food" refers to a food prepared and processed in the form of tablets, capsules, powders, granules, liquids and pills using raw materials or ingredients having useful functions for the human body. Here, the term "functionality" means obtaining useful effects on health purposes such as nutrient control or physiological effects on the structure and function of the human body. The health functional food of the present invention can be prepared by a method commonly used in the art, and the preparation can be prepared by adding raw materials and ingredients commonly added in the art. In addition, the formulation of the health functional food can also be prepared without limitation as long as the formulation is recognized as a health functional food. Food composition of the present invention can be prepared in various forms of formulation, unlike the general medicine has the advantage that there is no side effect that can occur when taking a long-term use of the drug as a food raw material, excellent portability, Health functional foods can be taken as a supplement to prevent or improve oral disease.

There is no restriction on the form that the health functional food of the present invention can take, and may include all foods in a general sense, and may be mixed with terms known in the art such as functional foods. In addition, the health functional food of the present invention may be prepared by mixing known additives with other appropriate auxiliary ingredients that may be included in the food according to the choice of those skilled in the art. Examples of foods that can be added include meat, sausages, breads, chocolates, candy, snacks, confectionery, pizza, ramen, other noodles, gums, dairy products, including ice cream, various soups, beverages, teas, drinks, alcoholic beverages and There is a vitamin complex, etc., can be prepared by adding to the juice, tea, jelly and juice prepared with the ginkgo extract according to the present invention as a main component. It also includes foods used as feed for animals.

The quasi-drug composition containing the ginkgo extract of the present invention as an active ingredient is excellent in preventing or improving oral disease. Specifically, the composition of the present invention is excellent in antimicrobial activity against dental caries and periodontal disease-inducing bacteria, concentration-dependent inhibition of pain and inflammation markers, inhibition of gingivitis formation, symptom suppression effect and bad breath removal effect Excellent in utilization as a quasi-drug composition for the prevention or improvement of one or more oral diseases selected from the group consisting of dental caries, periodontal disease, toothache, syringe and bad breath. In addition, the composition comprising the ginkgo extract of the present invention can be used as a pharmaceutical composition and a food composition.

The following drawings, which are attached to this specification, illustrate preferred embodiments of the present invention, and together with the contents of the present invention serve to further understand the technical spirit of the present invention, the present invention is limited to the matters described in such drawings. It should not be construed as limited.
Figure 1 shows the results of confirming the effect of inhibiting PGE2 by treating LPS (control group) and LPS and ginkgo extract (experimental group) as inflammatory stimuli to macrophages, respectively.

Hereinafter, the configuration and effects of the present invention through the embodiments will be described in more detail. These examples are only for illustrating the present invention, but the scope of the present invention is not limited by these examples.

Example 1: Preparation of Ginkgo Extract

Ginkgo biloba extract was added to ethanol in a pulverized or cut ginkgo dry sample, sonicated for 15 minutes, and left to stand for 2 hours at room temperature. After concentration, the sample frozen in a deep freezer was obtained by drying in a freeze dryer. The ginkgo extract obtained as described above was used by dissolving in 0.5% CMC (carboxy methylcellulose) solution and DMSO (dimethyl sulfoxide), respectively.

Experimental Example 1: Antibacterial effect on dental caries and periodontitis causing bacteria

Antibacterial activity experiments were performed to confirm the preventive or therapeutic effect of the ginkgo extract against dental caries and periodontitis. To determine the effect of inhibiting the growth of oral pathogens, the antimicrobial activity test was performed by using the paper disk test using Streptococcus mutans , the representative bacteria of dental caries, and Porphyromonas gingivalis , the representative bacteria of periodontal disease. Was carried out.

After increasing the activity of the oral pathogens in the optimum culture conditions shown in Table 1 below, the culture medium was incubated for 4 to 6 hours in the optimum medium of each bacterium to increase the turbidity of the culture medium. 0.5 (1.5 × 10 ⁸ ) was adjusted and 0.1 ml of each oral pathogen was evenly spread on a plate medium. Thereafter, the sterile paper disk was inoculated with the ginkgo extract at a concentration of 10 mg / disc, followed by absorption drying for 1 hour. Then, after culturing for 24 to 48 hours at the optimum temperature of each oral pathogen, the size (diameter mm) of growth-lowering ring was measured, and the results are shown in Table 2 below.

Strain Optimal condition Gram Dyeing
Gram staining Strain name Temperature badge characteristics Gram (+) Streptococcus mutans 37 ℃ BHI Facultative anaerobic Gram (-) Porphyromonas gingivalis 37 ℃ TSA Hemin Menadione Badge Anaerobic

Determination of Antimicrobial Activity of Ginkgo Biloba Extract against Oral Pathogens Growth ring ring diameter (mm) Streptococcus mutans Porphyromonas gingivalis No treatment -(Growth not blocked) -(Growth not blocked) Ginkgo extract treatment 11.9 12.0

As shown in Table 2 above, compared to the untreated group not treated with the ginkgo extract, the group treated with the ginkgo extract has Streptococcus mutans having a growth-lowering ring diameter of about 12.0 mm for the two oral pathogens. And fairly good antimicrobial activity against Popiromonas gingivalis. Therefore, it was found that the ginkgo extract can be used for preventing or treating dental caries or periodontal disease.

Experimental Example 2 Inhibitory Effect of Gingivitis Formation

In order to confirm the effect of preventing or treating gingivitis of the ginkgo extract, a test subject was selected and a clinical experiment was performed.

First, sodium carboxymethylcellulose, sodium lauryl sulfate, glycerin, colloidal silicon dioxide, silicas, sodium cocoyl isocyanate, dodysine and sweeteners, fragrances, coloring agents, etc., which are generally used in preparing toothpaste Toothpaste was made to the control toothpaste, experimental toothpaste was prepared by containing 0.01% by weight of the ginkgo extract in the control toothpaste.

In order to screen the subjects, 30 oral patients were examined by age 30 years from age 30 to age 50 in gingivitis patients with even teeth and no missing teeth. After screening, 60 patients were divided into clinical trials to treat gingival inflammation.

Specifically, the test subjects were divided into a control group and a test group, and the control group was trained to use the prepared control toothpaste three times a day before going to sleep 2 hours after eating, and the experimental group used the prepared test toothpaste three times a day at the same time. To be educated. After the periodontal smearing was performed to score the initial gingivitis index, and the control group to use the prepared control toothpaste, the experimental group to use the prepared experimental toothpaste 1 week, 1 month, 3 months and 6 months after oral examination The gingivitis index was checked. The gingivitis index is measured by inserting a periodontal probe into the gingival bulb and burning the probe around each tooth without applying force and measuring the bleeding state after 30 seconds. Scores were recorded according to the results shown in Table 4.

Based on gingivitis index score Contents 0 points Bleeding 1 point Normal bleeding state 2 points Glandular bleeding 3 points Interdental triangle bleeding 4 points Total gingival bleeding

Gingivitis inhibitory effect time Control Experimental group Week 0 1.04 1.03 1 week 1.60 1.03 1 month 2.69 1.04 3 months 2.77 1.04 6 months 2.85 1.05

As shown in Table 4, when compared to the control group, the experimental group using the toothpaste containing the ginkgo extract, even after 6 months to maintain a normal bleeding state and continued gingival inhibitory effect. Therefore, it was found that the ginkgo extract can be used for the purpose of preventing or treating gingivitis.

Experimental Example 3 Inhibitory Effect of PGE2, a Pain and Inflammation Marker

In order to confirm the effect of preventing or treating toothache of the ginkgo extract, the inhibitory effect of PGE2, known as a pain and inflammation marker, was confirmed.

First, macrophages were inoculated at a concentration of 1.5 X 10 ⁵ cells / ml in DMEM medium containing 10% FBS and incubated in a 24 well plate at 37 ° C. and 5% CO ₂ for 24 hours. Then, after incubating for 24 hours by treating LPS 1 ㎍ / ml and ginkgo biloba extracts by concentration, the PGE2 ELISA assay kit (Thermo SCIENTIFIC) was purchased and supernatant was used to inhibit PGE2 inhibition according to the concentration of ginkgo extract Analyzes (FIG. 1).

As shown in Figure 1, compared to the group treated with LPS only, 50 ppm ginkgo extract up to about 80%, 100 ppm ginkgo extract was effective to inhibit PGE2 up to about 95%. Therefore, it was found that the ginkgo extract can be used for the purpose of preventing or treating toothache.

Experimental Example 4: Effect of Seroin Inhibition

In order to confirm the prophylactic or therapeutic effect of the sirens of the ginkgo extract, clinical trials were performed by selecting test subjects, and the control toothpaste and the experimental group toothpaste used in the clinical trial were prepared in the same manner as the toothpaste of Experimental Example 2, respectively.

Subjects were 40 volunteers who had dentin hypersensitivity teeth and agreed to participate in the trial, with a total of 80 subjects. In addition, 20 males and 20 females were 20 to 50 years old. The subjects were not aware of the toothpaste contents and the total duration of the experiment was 2 weeks.

The experiment was performed by measuring the response of the subject after applying a temperature stimulus. Before conducting the experiment, check the sensitive areas of the dentin hypersensitivity teeth of each subject in advance, and drop the cold water of about 5 ° C with an eyedropper on the aerobic area of the tooth with a dropper, and then scored according to the criteria shown in Table 5 below. In the control toothpaste, the experimental group was allowed to use the experimental toothpaste three times a day for two weeks, and after two weeks, drop the cold water of about 5 ° C with an eyedropper to score. Statistical treatment was tested before and after the experiment stimulation scores by paired Student-t test (paired Student-t test), the response scores after 2 weeks for temperature stimulation is shown in Table 6 below.

Score criteria for subjects after stimulation score Contents 0 points There is no discomfort. 1 point Slightly uncomfortable or cold 2 points Sour 3 points pain.

Sirin degree of inhibition time Control Experimental group Week 0 2.15 ± 0.22 2.15 ± 0.12 2 weeks 2.13 ± 0.20 1.69 ± 0.19 ^* p> 0.05 * p <0.05

※ p> 0.05: 95% confidence, no statistically significant difference. * p <0.05: 95% confidence, statistically significant difference.

As shown in Table 6, when compared with the control group, the experimental group using the toothpaste containing the ginkgo extract was suppressed after two weeks. Therefore, it was found that the ginkgo extract can be used for the purpose of preventing or treating syringes.

Experimental Example 5: Bad breath removal effect

In order to confirm the effect of preventing or treating bad breath of the ginkgo extract extracts were subjected to clinical trials, control toothpaste and experimental group toothpaste used in the clinical trial were prepared in the same manner as the toothpaste of Experimental Example 2, respectively.

As a test subject, 50 men and women without dental caries were selected and the cross-over test was performed on the control toothpaste and the test group toothpaste. Commercial garlic powder was dispersed in water, left to stand for 24 hours, and diluted to make a Halimeter measurement value of 700 ppb or more, and the diluent was used as a source of bad breath. The subjects were gargled with 15 ml of garlic powder for 30 seconds, and after 1 minute, the degree of bad breath was measured with a halimeter. . After 1 minute, 5 minutes, and 30 minutes after brushing the teeth, the degree of bad breath was measured by measuring the degree of bad breath. The results were shown in Table 7 below.

Bad breath removal effect time Control Experimental group 1 minute 54.9% 98.3% 5 minutes 47.5% 96.2% 30 minutes 38.7% 93.9%

As shown in Table 7, when compared with the control group, the experimental group using the toothpaste containing the ginkgo extract, the bad breath was removed to about 98% after brushing, even after 30 minutes after brushing the bad breath removal rate of more than 93% The removal effect was much better than the control. Therefore, it was found that the ginkgo extract can be used for the purpose of preventing or treating bad breath.

From the above description, those skilled in the art will appreciate that the present invention can be implemented in other specific forms without changing the technical spirit or essential features. In this regard, the embodiments described above are to be understood in all respects as illustrative and not restrictive. The scope of the present invention should be construed that all changes or modifications derived from the meaning and scope of the following claims and equivalent concepts rather than the detailed description are included in the scope of the present invention.

Claims (14)

A quasi-drug composition for oral disease prevention or improvement comprising ginkgo biloba extract as an active ingredient.
According to claim 1, wherein the oral disease is dental caries, quasi-drug composition.
The quasi-drug composition according to claim 2, wherein the dental caries is caused by Streptococcus mutans .
According to claim 1, wherein the oral disease is periodontal disease, quasi-drug composition.
The quasi-drug composition according to claim 4, wherein the periodontal disease is periodontitis or gingivitis.
The quasi-drug composition of claim 4, wherein the periodontal disease is caused by Porphyromonas gingivalis .
According to claim 1, wherein the oral disease is toothache, quasi-drug composition.
The quasi-drug composition according to claim 1, wherein the oral disease is syringy.
The quasi-drug composition according to claim 1, wherein the oral disease is bad breath.
The quasi-drug composition according to claim 1, wherein the quasi-drug is for oral use.
The method of claim 10, wherein the dosage form of the quasi-drug composition is one or more formulations selected from the group consisting of toothpaste, mouthwash, mouthwash, gum, candy, oral spray, oral ointment, oral varnish, oral toothpaste and gum massage cream. It will be quasi-drug composition.
Pharmaceutical composition for the prevention or treatment of oral diseases, including ginkgo biloba extract as an active ingredient.
Food composition for preventing or improving oral diseases, including ginkgo biloba extract as an active ingredient.
Oral composition comprising Ginkgo biloba extract as an active ingredient.

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