KR102574859B1 - Composition for prevention or treatment of oral disease comprising Ginkgo biloba extract - Google Patents

Abstract

The present invention relates to a composition for preventing or treating oral diseases containing a ginkgo biloba extract, and more particularly, to a quasi-drug composition having an effect of preventing or improving dental caries, periodontal disease, toothache, ache, and bad breath. The composition of the present invention has excellent antibacterial activity against bacteria causing dental caries and periodontal disease, inhibits PGE2, a pain and inflammation marker, in a concentration-dependent manner, has excellent effects on inhibiting gingivitis formation, inhibiting symptoms of syringitis, and removing bad breath. It is highly applicable as a quasi-drug composition for preventing or improving one or more oral diseases selected from the group consisting of dental caries, periodontal disease, toothache, ache, and bad breath. In addition, the composition of the present invention can be used as a pharmaceutical composition and food composition.

Description

Composition for preventing or treating oral disease comprising ginkgo extract {Composition for prevention or treatment of oral disease comprising Ginkgo biloba extract}

The present invention relates to a composition for preventing or treating oral diseases containing a ginkgo biloba extract, and more particularly, to a quasi-drug composition having an effect of preventing or improving dental caries, periodontal disease, toothache, ache, and bad breath. In addition, the present invention relates to a pharmaceutical composition containing a ginkgo extract, a food composition, and an oral composition containing a ginkgo extract.

Healthy teeth are one of the five blessings and are an important factor in determining the quality of life. Among oral diseases, dental cavities (cavities) and periodontal diseases (periodontal diseases) are diseases with high incidence worldwide, and various clinical symptoms such as pain, masticatory dysfunction, destruction of periodontal tissue, bad breath and cold ears It is known as the main factor that causes tooth loss and tooth loss, and the causative factors of oral disease are increasing due to changes in eating habits.

About 600 to 800 or more microorganisms are known to exist in the human oral cavity, and these microorganisms are controlled by enzymes such as lysozyme secreted by saliva. However, the oral environment rich in nutrients and moisture is a favorable condition for the growth of microorganisms, and the tongue or dental plaque provides an excellent habitat for microorganisms. Some of these microorganisms are opportunistic pathogens and cause diseases such as dental caries, periodontal disease, and tooth decay (dentin hypersensitivity) and bad breath.

There are antibacterial agents as a method of inhibiting the growth of oral pathogens that live in dental plaque and cause dental caries, periodontal disease, bad breath, and cold teeth. It has been developed as an inhibitor and treatment agent for periodontal disease, dental pulp and apical infection.

However, since antibiotics can cause the appearance of resistant bacteria in the oral cavity and superinfection along with systemic side effects on our body, long-term use is difficult and can only be used as a therapeutic agent. In addition, antibacterial agents used in mouthwash include Sangquinarine, Listerine, Peroxide, Chlorhexidine, etc. The treatment effect on periodontal disease is not only unclear, but also has a high price. Listerine has alcohol as its main component and has a slight bacteriostatic effect. There are possible downsides. In addition, pyroxide, which has recently been added for a whitening effect, is toxic to bacteria, but at the same time it is toxic to human tissues, resulting in safety problems and sometimes bacteria resistant to pyroxide. In addition, chlorhexidine is known to be the best among known agents for preventing and treating periodontal disease as well as inhibiting plaque formation, but causes tissue irritation, discoloration and degeneration of tissues, and has side effects such as a particularly pungent taste and strong odor. In addition to the problems indicated, there are disadvantages that cannot be used for a long period of time for the purpose of treatment or prevention, such as causing mycosis and carcinogenicity, limiting its use in the case of pregnant women.

On the other hand, Ginkgo biloba extract is a component obtained by extracting from the fruit of the ginkgo tree. The ginkgo tree belongs to the deciduous tree and is distributed throughout the country except for high mountains in the north, highlands, and places with low temperatures. Ginkgo contains nutrients such as carotene and vitamin C.

Under this background, the present inventors have conducted research to derive natural materials that are effective in preventing and improving oral diseases such as dental caries, periodontal disease, toothache, cold teeth, and bad breath without worrying about the side effects described above. . As a result, as a natural-derived composition that is safe and can be used safely in the body, ginkgo extract exhibits antibacterial effects against dental caries and periodontal disease-causing bacteria, PGE2 inhibitory effects, gingivitis formation inhibitory effects, cold tooth inhibitory effects, and bad breath removal effects. , and came to complete a quasi-drug composition, a pharmaceutical composition, a food composition, and an oral composition using the same.

Korean Patent Publication No. 10-2015-0050239

An object of the present invention is to provide a quasi-drug composition for preventing or improving oral diseases comprising ginkgo extract as an active ingredient.

Another object of the present invention is to provide a pharmaceutical composition for preventing or treating oral diseases comprising ginkgo extract as an active ingredient.

Another object of the present invention is to provide a food composition for preventing or improving oral diseases comprising ginkgo extract as an active ingredient.

Another object of the present invention is to provide an oral composition comprising ginkgo extract as an active ingredient.

The present invention provides a quasi-drug composition for preventing or improving oral diseases containing ginkgo biloba extract as an active ingredient in order to solve the above problems and achieve the object of the present invention.

As another aspect of the present invention, there is provided a pharmaceutical composition for preventing or treating oral diseases comprising ginkgo extract as an active ingredient.

As another aspect of the present invention, there is provided a food composition for preventing or improving oral diseases comprising ginkgo extract as an active ingredient.

As another aspect of the present invention, an oral composition comprising ginkgo biloba extract as an active ingredient is provided.

The present invention provides a quasi-drug composition containing ginkgo extract as an active ingredient.

In the present invention, "Ginkgo biloba extract" is a component obtained by extracting from the fruit of the ginkgo tree. It is distributed nationwide, and ginkgo contains nutrients such as carotene and vitamin C.

The present invention is not particularly limited to the method of obtaining the ginkgo extract, and chemically synthesized by methods known in the art or commercially available materials may be used.

As used herein, the term "extract" refers to a liquid component obtained by immersing a desired substance in various solvents and then extracting at room temperature, low temperature or elevated temperature for a certain period of time, and removing the solvent from the liquid component. It means the result, such as the obtained solid content. In addition, in addition to the above results, it can be comprehensively interpreted as including all dilutions of the results, concentrates thereof, adjusted products, and purified products thereof.

In the present invention, the extract means ginkgo extract. The ginkgo extract can be obtained by extraction with water or various organic solvents. At this time, the organic solvent used is not particularly limited thereto, as long as an extract having an effect of preventing or treating oral diseases can be obtained, but specifically, it may be water, a polar solvent or a non-polar solvent, and more specifically, water, It may be a lower alcohol having 1 to 6 carbon atoms (methanol, ethanol, propanol or butanol, etc.), a mixed solvent thereof, and most specifically, ethanol or a mixed solvent thereof.

In addition, the method for obtaining the extract is not particularly limited thereto, as long as the extract having an effect of preventing or treating oral diseases can be obtained, but specifically, the ginkgo biloba fruit, leaves, dried products, processed products, etc. Methods such as immersion in a solvent and extraction at room temperature, cold extraction, extraction by heating at 40 to 100, ultrasonic extraction by applying ultrasonic waves, and reflux extraction using a reflux condenser may be used.

The present invention provides a quasi-drug composition for preventing or improving oral diseases comprising the ginkgo extract as an active ingredient.

Further, specifically, the present invention provides a quasi-drug composition for preventing or improving at least one oral disease selected from the group consisting of dental caries, periodontal disease (periodontitis or gingivitis), toothache, ache, and bad breath, comprising the ginkgo extract as an active ingredient. can provide

In the present invention, "oral disease" refers to various diseases occurring in the oral region, and the oral region refers to the space in the mouth connected to the pharynx from the front lip to the rear oral cavity. In the present invention, the oral disease is a concept that includes all diseases that occur in the oral cavity regardless of their symptoms, and non-limiting examples of the oral disease include dental caries, periodontal disease (periodontitis or gingivitis), toothache, cold teeth, bad breath. etc. can be mentioned.

In the present invention, "dental cavities" is an infectious disease caused by bacteria inhabiting the surface of teeth, which is also referred to as tooth decay, and shows symptoms of tooth hard tissue erosion and loss. Specifically, the milky-white, translucent, and hard substance that covers the surface of the head of the tooth and protects tooth dentin is called tooth enamel or enamel. Tooth decay is when the tooth enamel is damaged. The main cause of dental caries is dental plaque, a bacterial film formed on the surface of teeth. ( Streptococcus sobrinus ) Bacteria and Streptococcus mutans ( Streptococcus mutans ) Oral microorganisms such as attach to form a biofilm (dental plaque, plaque) is made and further thickened by Fusobacterium ( Fusobacterium ) lose Dental caries of the present invention includes all, regardless of the type, as long as the causative bacteria cause dental caries, specifically, Streptococcus mutans ( Streptococcus mutans ), Streptococcus sanguinis ( Streptococcus sanguinis ), Streptococcus sobrinus ( Streptococcus sobrinus ), Streptococcus ratti ( Streptococcus ratti ), Streptococcus criceti ( Streptococcus criceti ), Streptococcus anginosus ( Streptococcus anginosus ) and at least one homogeneous species selected from the group consisting of lactic acid bacteria, more specifically, Streptococcus It could be mutans.

The Streptococcus mutans is a type of streptococcus, a Gram-positive bacteria, and is also called a cavity-causing bacteria. The Streptococcus mutans proliferates only on the surface of the teeth, and primary teeth are not completed until around 30 months after birth. Therefore, until this time, it is difficult for mutans bacteria to proliferate as after infancy. If other oral bacteria are not infected with mutans from the mouth of adults during infancy, and other oral bacteria settle in the mouth, it is difficult for mutans bacteria to newly enter the already balanced oral ecosystem, and the probability of getting tooth decay after infancy is significantly increased. it gets lower If the causative bacteria have already been transmitted through the mouth of adults, frequent brushing and keeping the mouth clean will help prevent tooth decay. However, once mutans germs are established in the oral cavity, eradication of mutans germs is impossible.

In one embodiment of the present invention, as a result of measuring the size of the growth arrest rings after inoculating the discs containing the ginkgo biloba extract on the medium smeared with Streptococcus mutans, the diameter of the growth arrest rings in the experimental group containing the ginkgo extract was It was confirmed that the composition containing the ginkgo biloba extract could be used for preventing or improving dental caries, as it exhibited significantly excellent antibacterial activity against Streptococcus mutans at 11.0 mm or more (Table 2).

In the present invention, "periodontal disease" is a disease in which the gingiva, periodontal ligament, and bone tissue supporting the teeth are inflamed, and is often referred to as periodontitis, and depending on the severity of the disease, gingivitis and periodontitis divided into Gingivitis is a relatively mild form of periodontal disease with a rapid recovery, and the form limited to the gums, that is, soft tissues, is called gingivitis, and the case where such inflammation progresses to the gums and around the gum bones is called periodontitis. There is a V-shaped gap between the gingiva (gum) and the tooth, and oral pathogens attack the area below the gum line in this groove, releasing lipopolysaccharide (LPS), a inflammatory stimulus, resulting in Inflammation is created, such as swelling and bleeding of the gums, which damages the periodontal ligament and adjacent tissues. As periodontitis progresses, it damages the periodontal ligament and even the alveolar bone, eventually leading to tooth loss. Malnutrition such as protein and vitamins, pregnancy, hormonal disorders such as diabetes, smoking, and AIDS can aggravate the disease. In addition, other causes of periodontal disease include dental plaque and calculus. Periodontal disease of the present invention includes all, regardless of the type, as long as the causative bacteria cause periodontal disease, specifically Actinobacillus actinomycetemcomitans ( Actinobacillus actinomycetemcomitans ), Porphyromonas gingivalis ( Porphyromonas gingivalis ), other Rella Forsythia ( Tannerella forsythia ), Treponema denticola ( Treponema denticola ) and Fusobacterium nucleatum ( Fusobacterium nucleatum ) It may be at least one homogeneous species selected from the group consisting of, more specifically Porphyromonas ginseng It can be Balinese.

The Porphyromonas gingivalis is a type of Bacteroide smooth bacteria, and is a Gram-negative and anaerobic bacterium. The Porphyromonas gingivalis is found in the oral cavity where periodontal disease occurs, and is also found in the upper gastrointestinal tract, respiratory tract and colon. In patients with chronic periodontal disease, collagen is degraded by the collagenase enzyme of the bacteria, and the bacteria can invade gingival fibroblasts and survive even under a considerable concentration of antibiotics. In addition, the bacteria can survive for a long time by invading gingival epithelial cells.

In one embodiment of the present invention, as a result of measuring the size of the growth arrest ring after inoculating the disc containing the ginkgo biloba extract into a medium smeared with Porphyromonas gingivalis, the diameter of the growth arrest ring in the case of the experimental group containing the ginkgo extract It was confirmed that the composition containing the ginkgo biloba extract could be used for preventing or improving periodontal disease, as it exhibited significantly excellent antibacterial activity against Porphyromonas gingivalis with a diameter of 11.0 mm or more (Table 2).

In addition, in one embodiment of the present invention, as a result of conducting a clinical experiment by selecting test subjects with gingival inflammation, the experimental group using the experimental group toothpaste containing the ginkgo extract showed excellent gingival inflammation inhibitory effect and 6 months elapsed. It was confirmed that the composition containing the ginkgo biloba extract could be used for preventing or improving periodontal diseases including gingivitis (Table 4).

In the present invention, "toothache" refers to pain in the teeth when eating sweet food, or very cold or hot food. Pain is also included. It usually causes pain when chewing, swollen gums, and foul-smelling discharge. Toothache shows slightly different pain depending on the cause disease. Specifically, the pain caused by dental caries is initially painless, but gradually progresses and causes pain when the nerves in the tooth are deeply rotted. Pain is caused, and when a tooth is broken or cracked, when it touches cold food or bites hard, the tooth splits and stimulates the nerve, causing pain. In the case of pulpitis, pain is felt when cold food is touched in the early stage, and when the inflammation progresses, hot food is felt. Pain is caused by inflammation of the

In one embodiment of the present invention, as a result of confirming the PGE2 inhibitory effect known as a pain and inflammatory marker, the experimental group containing the ginkgo extract exhibited a concentration-dependent inhibitory effect on PGE2, so that the composition containing the ginkgo extract prevented toothache or It was confirmed that it can be used for improvement purposes (FIG. 1).

In the present invention, "cold tooth" refers to hypersensitive dentine, and the symptom of cold tooth refers to dentine hyperesthesia. Cold tooth is a sensitive feeling when the dentin part of the exposed tooth comes in contact with cold air or irritating food, and 60 to 98% of adults with periodontal disease show symptoms. Cold tooth symptoms are caused by bad brushing habits, excessive bite force, tooth decay on the gum side due to dissolution by acid, poor oral hygiene, periodontal treatment, restorative treatment, and tooth dissolution due to acidification. can appear As the root cause of this symptom, it appears when many tubules present in the dentin of the tooth are exposed to the outside. Exposure transmits all stimuli as they are to the nerves in the pulp, making them more sensitive than usual to the same stimuli and causing pain. there is. Symptoms of cold teeth can vary from mild symptoms to intense and continuous pain, and because teeth do not regenerate, taking painkillers or anti-inflammatory drugs is not a fundamental solution to the pain phenomenon. Symptoms of cold lice may appear throughout the entire tooth, or may be limited to a specific area, such as the upper or lower jaw, or the right or left side. The most frequently affected area varies depending on the cause, but mainly canines and small molars, and the area with the most severe pain is the cervical area, which is the boundary between the gums and teeth in more than 90%.

In one embodiment of the present invention, as a result of selecting test subjects with hypersensitive dentin teeth and conducting a clinical experiment, in the case of the experimental group using the experimental group toothpaste containing the ginkgo extract, the effect of suppressing symptoms was excellent, and the ginkgo extract was It was confirmed that the composition containing syrin can be used for preventive or ameliorative purposes (Table 6).

In the present invention, "halitosis" is a smell derived from the oral cavity and adjacent organs, and 85 to 90% of bad breath originates from the oral cavity, particularly from the back of the tongue. The main component of bad breath is volatile sulfur compounds, 90% of which are hydrogen sulfide made from cysteine and methyl mercaptan and dimethyl sulfide made from methionine. These components are mainly produced by protein enzymes secreted by anaerobic bacteria, and the back of the tongue becomes the most important habitat. This area is not well cleaned by saliva and has many small depressions, making it a place where bacteria live continuously. The production of volatile sulfur compounds by anaerobic bacteria is the most important cause of bad breath, but other oral diseases such as dental caries, periodontitis, and dry mouth are also caused. Many types of anaerobic bacteria are involved in generating bad breath, and a non-limiting example of bad breath causing bacteria is Porphyromonas gingivalis , which secretes many types and large amounts of enzymes.

In one embodiment of the present invention, as a result of conducting a clinical experiment on test subjects without dental caries, the experimental group using the experimental group toothpaste containing the Ginkgo biloba extract showed excellent bad breath removal effect, and the composition containing the Ginkgo biloba extract It was confirmed that it can be used for preventing or improving bad breath (Table 7).

The quasi-drug composition of the present invention may include a quasi-drug for oral use. Ingredients included in the quasi-drug composition of the present invention may include ingredients commonly used in oral quasi-drug compositions in addition to the above active ingredients as active ingredients, such as abrasives, wetting agents, binders, foaming agents, sweeteners, preservatives, medicinal ingredients, flavors agents, pigments, solvents, brighteners, solubilizers or pH adjusters.

The quasi-drug composition for oral use of the present invention can be prepared in any formulation commonly prepared in the art, for example, toothpaste, mouthwash, mouthwash, gum, candy, oral spray, oral ointment, and oral varnish. , oral rinse and gum massage cream, but may have formulations such as, but are not limited thereto.

As an example, when the oral quasi-drug composition of the present invention is a toothpaste formulation, it may contain a wetting agent, an abrasive, a binder, a foaming agent, a flavoring agent, a sweetening agent, a coloring agent, a preservative, a medicinal component, a solvent, a pH adjusting agent, and the like.

The humectant is to make the powder of toothpaste ingredients paste and prevent the toothpaste from hardening in the air, and glycerin, sorbitol, propylene glycol, polyethylene glycol, etc. alone or in combination of two or more, 1 to 60 weight of the total weight of the composition %, specifically, 10 to 50% by weight may be used.

The foaming agent diffuses the toothpaste into the oral cavity to increase the cleaning effect and acts as a surfactant to clean oral contamination. Alternatively, 0.5 to 10% by weight, specifically 0.5 to 5% by weight of the total weight of the composition may be used by mixing two or more kinds.

The binder prevents separation between powder and liquid components in toothpaste, and is a mixture of cellulose derivatives such as sodium carboxymethyl cellulose, methyl cellulose, and hydroxypropyl cellulose, sodium alginate, carrageenan, xanthan gum, etc. alone or in combination of two or more. 0.1 to 5% by weight, specifically 0.3 to 2% by weight of the total weight of the composition may be used.

The abrasive is calcium carbonate (CaCO ₃ ), calcium phosphate dibasic (CaHPO ₄ , CaHPO ₄ 2H ₂ O), silicic anhydride (SiO ₂ 2H ₂ O), aluminum hydroxide (Al(OH) ₃ ), potassium pyrophosphate, magnesium carbonate, etc. alone or in combination of two or more, and 1 to 60% by weight, specifically 10 to 50% by weight of the total weight of the composition can be used

The flavoring agent is to enhance the feeling of use by imparting a refreshing feeling and smell to the toothpaste, and peppermint oil, spearmint oil, menthol, etc. alone or in combination of two or more are used in an amount of 1 to 60% by weight, specifically 0.01 to 60% by weight of the total weight of the composition. 5% by weight can be used.

The sweetener is to remove the unpleasant taste caused by raw materials for toothpaste and improve the refreshing feeling, and 1 to 60% by weight of the total weight of the composition by mixing saccharic acid, aspartame, xylitol, licorice acid, etc. alone or in combination of two or more, specifically 0.01 to 5% by weight can be used.

The active ingredients are for the prevention of dental caries, periodontal disease, toothache prevention, cold lice prevention, bad breath removal, etc. Triclosan, tocopherol acetate, sodium monofluorophosphate, etc. may be used alone or in combination of two or more. In the present invention, ginkgo extract can be used as an additional active ingredient.

The quasi-drug composition for oral use of the present invention may be used alone or in combination, or may be used in combination with other quasi-drug compositions for oral use other than the present invention.

As another aspect of the present invention, a pharmaceutical composition comprising the ginkgo biloba extract as an active ingredient is provided. More specifically, the present invention is a pharmaceutical composition for preventing or treating at least one oral disease selected from the group consisting of dental caries, periodontal disease (periodontitis or gingivitis), toothache, ache, and bad breath, comprising the ginkgo extract as an active ingredient. can provide. Ginkgo biloba extract, oral disease, dental caries, periodontal disease, toothache, toothache and halitosis are as described above.

The pharmaceutical composition of the present invention is in the form of tablets, pills, powders, granules, capsules, suspensions, internal solutions, emulsions, syrups, aerosols, sterile injection solutions, etc. according to conventional methods for preventing and treating oral diseases. formulation is possible.

Solid preparations for oral administration include tablets, pills, powders, granules, capsules, etc. These solid preparations are prepared by mixing at least one excipient such as starch, calcium carbonate, sucrose, lactose, gelatin, etc. It can be. In addition to simple excipients, lubricants such as magnesium stearate and talc may also be used. Liquid formulations for oral administration include suspensions, internal solutions, emulsions, syrups, etc., and various excipients such as wetting agents, sweeteners, aromatics, and preservatives in addition to water and liquid paraffin, which are commonly used simple diluents. can be used

Preparations for parenteral administration may include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, suppositories, and the like. Propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate may be used as non-aqueous solvents and suspending agents.

In addition, the pharmaceutical composition of the present invention may further include a carrier, excipient or diluent. Carriers, excipients or diluents include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, gum acacia, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, hydroxyl Mineral oils such as propyl methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, and silicon dioxide may be used. there is.

The specific dosage of the pharmaceutical composition according to the present invention depends on factors such as formulation method, patient's condition and weight, patient's sex, age, disease severity, drug type, administration route and duration, excretion rate, response sensitivity, etc. It can be variously selected by those skilled in the art, and the dosage and frequency do not limit the scope of the present invention in any way.

The pharmaceutical composition of the present invention can be administered to mammals such as rats, mice, livestock, and humans through various routes. Any mode of administration can be envisaged, for example by oral, intravenous, intramuscular or subcutaneous injection.

As another aspect of the present invention, the present invention provides a food composition comprising the ginkgo extract as an active ingredient. Further, specifically, the present invention provides a food composition for preventing or improving at least one oral disease selected from the group consisting of dental caries, periodontal disease (periodontitis or gingivitis), toothache, ache, and bad breath, comprising the ginkgo extract as an active ingredient. can provide Ginkgo biloba extract, oral disease, dental caries, periodontal disease, toothache, toothache and halitosis are as described above. The food composition may be used in the form of health functional food, but is not limited thereto.

The ginkgo extract included in the food composition of the present invention may be included in the form of animals and plants including ginkgo extract, extracts thereof, fractions thereof, or processed products thereof. In addition, the composition may include a food additive that is acceptable in food science in addition to the active ingredient.

In the present invention, "food supplement additive" refers to a component that can be added to food supplementally, and can be appropriately selected and used by those skilled in the art as being added to prepare a health functional food of each formulation. Examples of food additives include various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, colorants and fillers, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners , pH regulators, stabilizers, preservatives, glycerin, alcohol, carbonation agents used in carbonated beverages, etc. are included, but the types of food additives of the present invention are not limited by the above examples.

The food composition of the present invention may include health functional food. In the present invention, "health functional food" refers to food manufactured and processed in the form of tablets, capsules, powders, granules, liquids and pills using raw materials or ingredients having useful functionality for the human body. Here, “functionality” means obtaining useful effects for health purposes, such as adjusting nutrients for the structure and function of the human body or physiological functions. The health functional food of the present invention can be prepared by a method commonly used in the art, and can be prepared by adding raw materials and components commonly added in the art during the preparation. In addition, the formulation of the health functional food may also be manufactured without limitation as long as the formulation is recognized as a health functional food. The food composition of the present invention can be prepared in various types of formulations, and unlike general drugs, it has the advantage of not having side effects that may occur when taking drugs for a long time using food as a raw material, and has excellent portability, so that the composition of the present invention Health functional food can be consumed as a supplement to prevent or improve oral diseases.

There is no limit to the form that the health functional food of the present invention can take, and it can include all foods in a conventional sense, and can be used interchangeably with terms known in the art, such as functional foods. In addition, the health functional food of the present invention can be prepared by mixing suitable other auxiliary ingredients and known additives that can be included in food according to the selection of those skilled in the art. Examples of food that can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, chewing gum, dairy products including ice cream, various soups, beverages, tea, drinks, alcoholic beverages, and There are vitamin complexes, etc., and it can be prepared by adding the ginkgo extract according to the present invention to juice, tea, jelly, juice, etc. prepared as a main component. It also includes food used as feed for animals.

The quasi-drug composition comprising the ginkgo biloba extract of the present invention as an active ingredient has an excellent effect of preventing or improving oral diseases. Specifically, the composition of the present invention has excellent antibacterial activity against bacteria causing dental caries and periodontal disease, inhibits PGE2, a pain and inflammatory marker, in a concentration-dependent manner, inhibits gingivitis formation, inhibits symptoms of ache, and removes bad breath. It is excellent and has high utilization as a quasi-drug composition for preventing or improving one or more oral diseases selected from the group consisting of dental caries, periodontal disease, toothache, ache and halitosis. In addition, the composition containing the ginkgo extract of the present invention can be used as a pharmaceutical composition and a food composition.

The following drawings attached to this specification illustrate preferred embodiments of the present invention, and serve to further understand the technical idea of the present invention together with the contents of the above-described invention, so the present invention is limited to those described in the drawings. It should not be construed as limiting.
Figure 1 shows the results of confirming the PGE2 inhibitory effect by treating macrophages with LPS (control group) and LPS and ginkgo extract (experimental group), respectively, which are inflammatory stimulants.

Hereinafter, the configuration and effects of the present invention will be described in more detail through examples. These examples are only for illustrating the present invention, and the scope of the present invention is not limited by these examples.

Example 1: Preparation of ginkgo extract

Ginkgo biloba extract is obtained by adding ethanol to a crushed or cut ginkgo dry sample, sonicating for 15 minutes, and then allowing it to stand at room temperature for 2 hours 10 times a day. After concentration, samples frozen in a deep freezer were obtained by drying in a freeze dryer. The ginkgo extract obtained as described above was dissolved in 0.5% CMC (carboxy methylcellulose) solution and DMSO (dimethyl sulfoxide) for use in the experiment.

Experimental Example 1: Antibacterial effect on dental caries and periodontitis causative bacteria

An antibacterial activity test was performed to confirm the preventive or therapeutic effect of the ginkgo extract on dental caries and periodontitis. To confirm the effect of inhibiting the growth of oral pathogens, Streptococcus mutans , a representative bacterium causing dental caries, and Porphyromonas gingivalis , a representative bacterium causing periodontal disease, were used to test antibacterial activity by using a paper disk test method. was carried out.

After increasing the activity of each of the oral pathogens under the optimal culture conditions of Table 1, they were cultured in the optimal medium for each bacteria for about 4 to 6 hours, and the turbidity of the culture medium was Macfarland turbidity No. 0.5 (1.5 X 10 ⁸ ), and 0.1 ml of each oral pathogen was evenly spread on a plate medium. Thereafter, the sterilized paper disk was inoculated with the ginkgo extract at a concentration of 10 mg/disc and then absorbed and dried for 1 hour. Then, after culturing for 24 to 48 hours at the optimal temperature of each oral pathogen, the size (diameter mm) of the growth arrest ring was measured, and the results are shown in Table 2 below.

strain optimum conditions Gram stain
(Gram staining) strain name temperature badge characteristics Gram (+) Streptococcus mutans 37 ℃ BHI facultative anaerobic Gram (-) Porphyromonas gingivalis 37 ℃ TSA Hemin Menadione Badge anaerobic

Antibacterial activity measurement result of ginkgo extract against oral pathogens Growth arrest ring diameter (mm) Streptococcus mutans Porphyromonas gingivalis untreated - (Growth is not inhibited) - (Growth is not inhibited) ginkgo extract processing 11.9 12.0

As shown in Table 2, compared to the non-treated group not treated with the ginkgo extract, the group treated with the ginkgo extract had a diameter of about 12.0 mm of growth inhibitory rings for the two oral pathogens, Streptococcus mutans and Porphyromonas gingivalis showed significantly excellent antibacterial activity. Therefore, it was found that the ginkgo extract can be used for preventing or treating dental caries or periodontal disease.

Experimental Example 2: Gingivitis Formation Inhibiting Effect

In order to confirm the effect of the ginkgo extract in preventing or treating gingivitis, a clinical experiment was conducted by selecting a test subject group.

First, sodium carboxymethylcellulose, sodium lauryl sulfate, glycerin, colloidal silicon dioxide, silica, sodium cocoyl isethionate, dodicin and sweeteners, fragrances, and coloring agents commonly used in the manufacture of toothpaste are used. A control toothpaste was prepared, and an experimental toothpaste was prepared by containing 0.01% by weight of the ginkgo extract in the control toothpaste.

In order to select test subjects, detailed oral examinations were conducted on 30 patients by gender at 10-year intervals from 30 to 50 years of age for gingivitis patients with even alignment and no missing teeth. Then, 120 test subjects were selected. After screening, 60 people were divided into gingival inflammation treatment effects, and clinical trials were conducted as follows.

Specifically, the experimental group was divided into a control group and an experimental group, and the control group was trained to use the prepared control toothpaste 3 times a day before going to bed after 2 hours after eating, and the experimental group used the prepared experimental group toothpaste 3 times a day at the same time. trained to do Then, a toothbrush was performed to score the initial gingivitis index, and the control group used the prepared control toothpaste, and the experimental group used the prepared experimental group toothpaste, and oral examination was performed after 1 week, 1 month, 3 months and 6 months. The gingivitis index was examined. The method of measuring the gingivitis index is to insert a periodontal probe into the gingival sulcus, burn around each tooth without applying force, and measure the state of bleeding after 30 seconds. Scores were recorded according to, and the results are shown in Table 4.

Gingivitis index criteria score detail 0 points no bleeding 1 point normal bleeding 2 points glandular hemorrhage 3 points Interdental triangular bleeding condition 4 points Total gingival bleeding

Inhibition of gingival inflammation hour control group experimental group 0 weeks 1.04 1.03 1 week 1.60 1.03 1 month 2.69 1.04 3 months 2.77 1.04 6 months 2.85 1.05

As shown in Table 4, compared to the control group, the experimental group using the toothpaste containing ginkgo extract maintained a normal bleeding state even after 6 months, and the gingival inflammation inhibitory effect continued. Therefore, it was found that the ginkgo extract can be used for preventing or treating gingivitis.

Experimental Example 3: Inhibiting effect of PGE2, a marker of pain and inflammation

In order to confirm the effectiveness of the ginkgo biloba extract for preventing or treating toothache, the PGE2 inhibitory effect known as a pain and inflammation marker was confirmed.

First, macrophages were inoculated at a concentration of 1.5 X 10 ⁵ cells/ml in DMEM medium containing 10% FBS, and cultured in a 24 well plate at 37°C and 5% CO ₂ for 24 hours. Then, 1 μg/ml of LPS and Ginkgo biloba extract, which are inflammation-inducing stimulants, were treated by concentration and further cultured for 24 hours. Then, a PGE2 ELISA assay kit (Thermo SCIENTIFIC) was purchased and the PGE2 inhibitory ability according to the concentration of Ginkgo biloba extract was measured using the supernatant. analyzed (FIG. 1).

As shown in FIG. 1, compared to the group treated with only LPS, the ginkgo extract at a concentration of 50 ppm inhibited PGE2 by about 80% and the extract at a concentration of 100 ppm inhibited PGE2 by about 95%. Therefore, it was found that the ginkgo extract can be used for preventing or treating toothache.

Experimental Example 4: Silin suppression effect

In order to confirm the preventive or therapeutic effect of the ginkgo biloba extract, a clinical experiment was conducted by selecting test subjects, and the control toothpaste and experimental group toothpaste used in the clinical experiment were prepared in the same manner as the toothpaste of Experimental Example 2.

The test subjects were 40 volunteers who agreed to participate in the experiment as people with hypersensitive dentin teeth, and a total of 80 teeth were tested. In addition, among the test subjects, 20 men and 20 women were aged from 20 to 50 years. The subjects were not allowed to know the contents of the toothpaste, and the total experiment period was 2 weeks.

The experiment was conducted by measuring the subject's response after applying a temperature stimulus. Before conducting the experiment, the hypersensitive area of the hypersensitive dentine tooth of each experiment subject was checked in advance, and cold water of about 5 ° C was dropped on the cold part of the tooth with a spide, and after scoring according to the criteria shown in Table 5, the control group The control toothpaste, the experimental group was allowed to use the experimental group toothpaste three times a day for 2 weeks, and after 2 weeks, cold water at about 5 ° C. was dropped again with a dropper to score. For statistical processing, the stimulation scores before and 2 weeks after the experiment were tested by a paired Student-t test, and the response scores after 2 weeks to the temperature stimulation are shown in Table 6 below.

Test subject's rating criteria after stimulation score detail 0 points no discomfort whatsoever 1 point slightly uncomfortable or cold 2 points chill a lot 3 points sick.

degree of suppression of syrin hour control group experimental group 0 weeks 2.15±0.22 2.15±0.12 2 weeks 2.13±0.20 1.69±0.19 ^* p>0.05 *p<0.05

※ p>0.05: 95% confidence, no statistically significant difference. *p<0.05: 95% confidence, statistically significant difference.

As shown in Table 6, compared to the control group, the experimental group using the toothpaste containing ginkgo extract suppressed the ache after 2 weeks. Therefore, it was found that the ginkgo extract can be used for preventing or treating syringitis.

Experimental Example 5: Bad breath removal effect

In order to confirm the effect of preventing or treating halitosis of the ginkgo extract, a clinical experiment was conducted by selecting test subjects, and the control toothpaste and experimental group toothpaste used in the clinical experiment were prepared in the same manner as the toothpaste of Experimental Example 2.

Fifty men and women without dental caries were selected as test subjects, and a cross-over test was conducted on the control group toothpaste and the experimental group toothpaste. Commercially available garlic powder was dispersed in water, allowed to stand for 24 hours, diluted so that the Halimeter measurement value was 700 ppb or more, and the diluted solution was used as a source of bad breath. The subjects gargled with 15 ml of diluted garlic powder for 30 seconds, and after 1 minute, the level of bad breath was measured with a Halimeter. The control group used the control toothpaste and the experimental group used the experimental group toothpaste, respectively, and brushed their teeth for 30 seconds to 1 minute. . After 1 minute, 5 minutes, and 30 minutes after brushing the teeth, the degree of bad breath was measured with a halimeter to determine whether or not the bad breath suppression continued, and the results are shown in Table 7 below.

Bad breath removal effect hour control group experimental group 1 min 54.9% 98.3% 5 minutes 47.5% 96.2% 30 minutes 38.7% 93.9%

As shown in Table 7, compared to the control group, the experimental group using the toothpaste containing ginkgo extract removed bad breath by about 98% after brushing their teeth, and the bad breath removal rate was 93% or more even after 30 minutes after brushing their teeth. The removal effect was far superior to that of the control group. Therefore, it was found that the ginkgo extract can be used for preventing or treating bad breath.

From the above description, those skilled in the art to which the present invention pertains will be able to understand that the present invention may be embodied in other specific forms without changing its technical spirit or essential features. In this regard, the embodiments described above should be understood as illustrative in all respects and not limiting. The scope of the present invention should be construed as including all changes or modifications derived from the meaning and scope of the claims to be described later and equivalent concepts rather than the detailed description above are included in the scope of the present invention.

Claims (14)

A quasi-drug composition for preventing or improving oral diseases comprising Ginkgo biloba extract as an active ingredient, wherein the oral disease is at least one selected from the group consisting of toothache and bad breath.
delete delete delete delete delete delete delete delete delete The method of claim 1, wherein the formulation of the quasi-drug composition is any one selected from the group consisting of toothpaste, mouthwash, mouthwash, gum, candy, oral spray, oral ointment, oral varnish, mouthwash, and gum massage cream. A formulation that is a quasi-drug composition.
A pharmaceutical composition for the prevention or treatment of oral diseases comprising Ginkgo biloba extract as an active ingredient, wherein the oral disease is at least one selected from the group consisting of toothache and halitosis.
A food composition for preventing or improving oral diseases, comprising Ginkgo biloba extract as an active ingredient, wherein the oral diseases are at least one selected from the group consisting of ache and bad breath. delete