KR20150027352A - Mouthwash Composition Using Natural Materials and the Manufacturing Method Thereof - Google Patents
Mouthwash Composition Using Natural Materials and the Manufacturing Method Thereof Download PDFInfo
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- KR20150027352A KR20150027352A KR20130104210A KR20130104210A KR20150027352A KR 20150027352 A KR20150027352 A KR 20150027352A KR 20130104210 A KR20130104210 A KR 20130104210A KR 20130104210 A KR20130104210 A KR 20130104210A KR 20150027352 A KR20150027352 A KR 20150027352A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
Abstract
Description
More particularly, the present invention relates to a composition for oral hygiene using a mixture of sun-dried salt, laurel leaf, millennium and green tea extract, and a method for preparing the same.
In general, mouthwash is widely used for prevention of tooth decay, halitosis, and periodontal disease caused by oral and dental damage caused by changes in eating habits of modern people. The ingredients of the mouthwash detergent used in this way are various, but the salt ingredient is the most abundant among them.
Although salt is usually used as a food in the diet, when salt management law was enacted in 1963, most of the salt in the world was salt, and it was separated into the world-class industrial standard classification minerals, Respectively.
On March 28, 2008, the company changed from minerals to foods to improve this point and to establish the order of distribution transaction of sun salt and to secure the safety as food.
In order to sterilize and disinfect the general oral cleaner, alcohol and antibiotics are used. Steroids are mixed for the purpose of anti-inflammation. Synthetic surfactants such as salicylic acid methylate and methol used in water pills and polyethylene glycol are used. Although it is not commercially available, it is said that 16 ~ 35% of the products are available in the domestic market. The American National Cancer Center reported a 25% increase in the chance of taking oral cancer when more than 25% of alcohol was used. In addition, the oral cleaning agent contains a small amount of purified salt.
As such, the mouthwash contains salt, but it is refined salt, and refined salt is less safe than sun saliva. The first country to develop refined salt is Japan. The salt of tablets has entered Korea in the early 1970s, and Japan is unable to produce salt and rock salt because most of the land is volcanic . So was earlier used to import from the salt produced in Australia and South America for food, the safety of these salts (still issues that still exist) By the problem, we developed a method of producing purified salt by the "last water exchange method ' This was an inevitable choice for Japan . Unlike domestic sun salt, which requires more wide torsion and production time, refined salt can be mass-produced at low cost in a short time, The problem is that most countries, except Korea, do not now use these refined salt for food, Even in a situation to avoid even the Japanese, refined salt, ion exchange membrane expression 'to provide a high-purity salt removal of impurities due to the use of impurity removal method. However, the down side is that there is no nutrients, such as minerals.
Because humans have eaten and digested only natural food for millions of years, the refined food is a completely new material that humankind has never experienced before, That's why our body can not accept the cleanest distilled water in the water. In other words, if we do not accept it in our body, there will be no harm, but our body recognizes and stores it as the same substance as the existing one, but in the end, Although safety and side effects have not been scientifically proven, This is the cause of concern for genetically modified foods. The recognition that refined salt is a chemical salt comes from a misunderstanding, Chemical catalysts and additives are not the only chemical foods . The separation and recombination of ions (+, -) is a chemical process, and when this process is done , the material is reborn as a totally different new material.
Oral detergents currently on the market prevent and treat tooth decay and periodontal disease by using sodium fluoride (NaF) and benzethonium chloride, which are antibacterial components. However, these main ingredients show side effects such as vomiting, nervous breakdown, coma, and diarrhea when swallowed, and cause fatal damage to the human body when swallowed excessively. Therefore, the use of children under the age of 7 is limited. Studies have been published that damage the mouth during long-term use. In addition, more than 10% of alcohol is usually added as a preservative. Alcohol is stimulant to stimulate the oral cavity and teeth, so that dentists are refraining from using it when there is an oral disease, and studies have been made that alcohol causes oral cancer.
In recent years, attempts have been made to add natural products as oral cleaning agent compositions. In accordance with this prior art, Korean Patent Laid-Open Publication No. 97-25598 discloses a method for removing odors of bad breath containing green tea extract containing bamboo salt and (+ Korean Patent Laid-Open Publication No. 96-20998 discloses an antibacterial oral cleaning agent composition containing pine needles extract, pine needles essential oil or a mixture thereof as an active ingredient, and pine needle extract is added as an oral detergent composition.
However, these prior art patents did not solve the problems of antimicrobial agents such as sodium fluoride and alcohol, because only green tea and pine needle extract were added to the existing oral cleaning composition (containing sodium fluoride and alcohol).
In addition, a variety of flavoring agents have been added to existing mouthwash compositions to remove bad breath, and this fragrance reduces halitosis by masking the bad breath. However, such a masking method can not be an effective removal method.
DISCLOSURE OF THE INVENTION The present invention has been made to solve the above problems and it is an object of the present invention to provide an oral cleansing agent using natural materials, and to provide an oral cleansing agent with an extractive mixture of sun salted salted salted salted salted salted salted salted salmon + Extracts of laurel leaf + mint to provide an oral cleaning agent. First, the most suitable sun salt according to age is selected to develop an oral cleaning agent combined with a natural product. Secondly, a part of the alcohol component, which is an ingredient of the oral cleaning agent, And third, relates to an oral care agent composition using natural materials and a method for manufacturing the same, which aims at contributing to the promotion of trust and health improvement of consumers by replacing chemicals, which are components of oral cleaning agents, with natural ones.
The present invention has been made to solve the above-mentioned problems,
1. Process for producing natural extract
(a) preparing a natural mouthwash composition by selecting a sun salt, a milky water, a mint, a green tea, and a laurel leaf;
(b) preparing ethanolic extracts of ethanol from the freeze-dried powder of the perennial stalks, and extracting them for 9 hours;
(c) adding distilled water to each of the prepared mint leaves, green tea leaves, and laurel leaves, and extracting the extracts for 9 hours;
2. Oral cleaning agent system process
(1) The oral cleaning agent is prepared by mixing the sun salt, 5% ethanol extract of mulberry seed and mint extract, mixing with boiling water and cooling the mixture.
(2) The oral cleaning agent is manufactured by mixing the sun salt, 5% ethanol extract of Tianjin early, and the extract of Laurel leaves and mixing with water boiled and boiled in the mixture.
(3) The oral cleaning agent is prepared by mixing the sun salt and 5% ethanol extract of green tea, green tea extract, laurel leaf extract and mint extract, mixing with boiled and cooled water.
By thus developing and providing an oral cleaning agent using the natural product of the present invention, it is possible to maintain the cleanliness of the mouth by suppressing the plaque forming bacteria of the teeth and inhibiting the activity of the oral cavity enzyme.
The present invention has the effect of contributing to the improvement of oral health of the people by replacing the alcohol component, which is a component of the oral detergent,
It has the effect of contributing to the promotion of trust and health promotion of consumers by replacing the chemical substance which is a component of the existing oral cleaning agent with natural material,
The development of oral cleaning products using sun salts promotes the consumption of sun salts, which has the effect of increasing the incomes of the salt seals,
It has the effect of improving the human relationship by increasing consumer confidence due to elimination of bad breath in the mouth.
Fig. 1 is a graph showing antibacterial measurement results against Streptococcus mutans, which is a mixture of three natural products (sun salt, mint, and millennia)
FIG. 2 is a graph showing the results of measuring the antimicrobial effect against Streptococcus mutans, a three-component mixture (sun-dried salt, green tea, laurel leaf)
FIG. 3 is a graph showing the results of measuring the antimicrobial effect of Streptococcus sorbinus on the cavities of five different mixed liquids (sun salt, green tea, laurel leaf, and mint)
FIG. 4 is a graph showing the results of antibacterial measurement against Streptococcus sorbinus of Cavidae by mixing three natural products (sun salt, mint, and millennia)
FIG. 5 is a graph showing the results of measurement of antimicrobial effect against Streptococcus sorbinus, a three-component mixture (sun dried salt, green tea, laurel leaf)
FIG. 6 is a graph showing the results of measuring antimicrobial effects against Streptococcus sorbinus with five mixed liquids (sun salt, green tea, laurel leaf, and mint)
FIG. 7 is a graph showing the result of antibacterial measurement against Haemophilus actinomycetemcomitans, which is a mixture of three natural products (sun salt, mint, and millennium)
FIG. 8 is a graph showing the results of measuring the antimicrobial effect against Haemophilus actinomycetemcomitans in three kinds of mixture liquids (sun salt, green tea, laurel leaves)
FIG. 9 is a graph showing the results of measuring the antibacterial effect against periodontal bacteria (Haemophilus actinomycetemcomitans) using 5 kinds of mixture liquids (sun salt, green tea, laurel leaf, mint).
Hereinafter, embodiments of the present invention will be described in detail.
In the present invention, in order to prepare an oral cleanser using a natural extract, it is necessary to prepare a composition of a natural product used as an oral cleanser, a step of preparing an extract by preparing a natural product, a step of mixing at least one of the prepared extracts with a sun salt, Thereby producing an oral cleaning agent.
1. Natural product extraction manufacturing process for manufacturing mouthwash
(A) preparing a natural material oral composition;
In the present invention, a natural product for preparing an oral cleaning agent is prepared. The natural product thereof is prepared as a natural product oral composition by selecting a sun-dried salt, a perennial stem, a mint leaf, a green tea leaf and a laurel leaf.
The prepared natural material oral composition is purchased from the market and used. Preferably, the perennial stem, mint leaf, green tea leaf, and laurel leaf are not dried, but may be dried when it is difficult to purchase the raw material.
(B) Step of producing ethanol extract of the early millennium
Of the prepared natural products, ethanol was added to the lyophilized powder of the perennial stem and extracted for 9 hours to prepare ethanol extract of the perennial plant.
At this time, freeze drying of the perennial stem is carried out by concentrating the perennial stem at intervals of 3 hours, lyophilizing it, and pulverizing it.
In the present invention, the technique of lyophilizing the concentrated extract and pulverizing it is a common technique, which is used to concentrate and extract the stems of the perennial plant, followed by lyophilization and pulverization.
500 g of ethanol powder is added to 50 g of the pulverized starch powder, and the mixture is extracted for 9 hours to prepare an extract.
In order to prepare the above-described tsunami seed extract, 50 g of the stem powder and 500 ml of ethanol are not limited. In one embodiment, when 100 g of the powder is milled, 1000 ml of ethanol is added and extracted for 9 hours to prepare an extract do.
Add 5% of the extracted ethanol extract to the boiled and cooled water, and thoroughly dissolve the extract.
(c) Preparation stage of mint leaf, green tea leaf, laurel leaf extract
300 ml of distilled water was added to each 30 g of mint leaf, green tea leaf and laurel leaf prepared in the step (a) of preparing the natural material oral composition, and each extract was prepared by extracting for 9 hours.
The extract is prepared by adding 30 g of each of mint leaves, green tea leaves, and laurel leaves to 300 ml of distilled water.
2. Oral cleaning agent manufacturing process
(1) Manufacture of mouthwash with ethanol and mint
2 g of the prepared sun salts, 4% of 5% ethanol extract of mulberry seed, and 200 of mint extract are mixed, and the mixture is boiled and cooled to prepare 100 ml of mouthwash.
(2) Manufacture of oral cleaning agent composed of ethanol extract of Tianjin early and laurel leaf extract
2 g of the prepared sun salts, 5 ml of 4% ethanol, 5 ml of ethanol extract, and 100 쨉 l of Laurel leaf extract are mixed and boiled and cooled to prepare 100 ml of mouthwash.
(3) Manufacture of mouthwash with green tea extract and laurel leaf extract
2 g of the prepared sun salts, 400 쨉 l of green tea extract, and 100 쨉 l of a laurel leaf extract are mixed, and the mixture is boiled and cooled to prepare 100 ml of mouthwash.
(4) Manufacture of mouthwash with ethanol, green tea extract, laurel leaf extract and mint extract
It is characterized in that 2 g of the prepared mannitol salt, 4 ml of 5% ethanol extract, 5 ml of green tea extract, 100 쨉 l of Laurel leaf extract and 200 쨉 l of mint extract are mixed and boiled and cooled water are mixed to prepare 100 ml of mouthwash .
In order to prepare each oral cleaning agent in the oral cleaning agent system, the amount of the substrate is a content ratio for producing 100 ml of the oral cleaning agent, and a large amount of the oral cleaning agent can be prepared by increasing the amount at the same ratio .
The salt used in the present invention is chlorine, magnesium, sodium, calcium, potassium and bromine in comparison with general refined salt. In particular, it is more effective than the salt of France 'Gerald' It is known that the mineral content of domestic sun-dried salt is higher and the nutritional content is superior.
Salt has long been effective in eliminating inflammation, and it is known that when the salt is concentrated in a small amount of water, especially in the case of oral inflammation, it causes strong osmotic pressure on the cell membranes of the germs so that the cell membranes of the germs are damaged and the bacteria are eradicated.
Also, when the eyes are congested, adjusting the concentration of the salt and putting it like eye drops disappears and the inflammation disappears. The base of all eye drops is used as salt water. In addition to this, when bronchial hygiene comes to the respiratory health such as tonsils, sore throat, cold, asthma, relief, bronchitis, pneumonia, pulmonary tuberculosis, it is necessary to clean the bronchi. As such, salt has the effect of treating inflammation and cleansing the oral cavity (Lee Insoo et al., Introduction to Salt, 2010).
In the modern society, as the society becomes more complicated and interpersonal relations become more and more due to the development of industry, there is a growing interest in the elimination of bad breath, which can cause discomfort to others. In this trend, oral care products such as mouthwashes and toothpastes have been developed and many researches and products aiming at prevention of dental caries, prevention of periodontal disease as well as removing bad breath have come out.
According to the 2000 survey by the Korea Institute of Health and Social Affairs, the importance of oral health has been emphasized as age increases (Choi Jung Soo, Jung Sawhwan, Development of strategy for promoting oral health. In addition, oral health knowledge was the most important as age increased, and dental caries and periodontal diseases were the most important oral diseases to be solved first.
In order to prevent tooth decay, dental caries and periodontal disease are the most common causes of tooth extraction in Korea (dental caries and periodontal diseases) (Kim Jongbae, Kim Kwang Soo, Kim Young Hee et al., Introduction to Public Oral Health, 3rd edition, Seoul, And the purpose of oral hygiene management is to remove the dental plaque membrane which is the cause of oral disease efficiently. It is said that the most effective way to control the oral hygiene is to brush the teeth, but it is not possible to manage the teeth with perfect brushing and the proper use of oral hygiene products should be appropriate for each patient (Kim, Jongbae, Clinical Prevention Dentistry. Seoul, Gongnam Temple).
Bad breath refers to an odor that causes discomfort to oneself or others through factors such as oral or respiratory system or otorhinolaryngeal disease, or systemic disease or oral environment (Kim, Jong Bae, et al., Clinical Prevention Dental 4th edition Seoul, Gospel. Bad breath can make an interpersonal relationship difficult in social life and can affect the quality of life of an individual, which is an important concern for the general public as well as those seeking medical care for bad breath treatment.
The cause of bad breath is divided into physiological causes, oral causes, and oral and psychological causes, most of which are known to be caused by oral causes (Rosenberg M. Bad breath: diagnosis and treatment. Univ Toronto D Dent J. 1990).
Of bad breath that occurs to cause the mouth is by anaerobic gram-negative bacteria present in the mouth, food particles, that occur while like the one eliminated oral mucosal epithelial cell, derived from the periodontal pockets white blood cells and the saliva corruption methyl mercaptan (CH 3 SH, It is known to be caused by volatile sulfur compounds (VSC) such as hydrogen sulfide (H 2 S) (Feller L. Blignaut E. Halitosis: a review, SADJ 2005) It is said that sodium fluoride is used in combination with 0.05% concentration, and it is effective in inhibiting or restraining the formation of plaque (Jongbae Kim, Yoo Jin Kim, Bae Dae Il et al. New Edition, Seoul, 1999, Adjunct Professor).
However, there are preventions and limitations on bad breath caused by multiple causes. A method of physically removing the bacterial matrix on the surface of the oral mucosa and teeth including the tongue to reduce oral malodor (Adams et al., 2004) and a method of removing odor components including VSC (Park JH, Han KS, Kim MK Effect of tongue scraping, Zncl mouth rinse, and perildontal treatment on the reduction of oral malodor Kor J Oral Med 2000). It is common to use antimicrobial substances that can chemically inhibit the growth and proliferation of bad breath-producing microorganisms, and the constituents of currently marketed oral functional agents are chemically synthesized products for long-term use and for similar purposes A variety of mouthwashes have been developed, such as Benzethonium Chloride and Methyparaben, which can cause side effects such as vomiting, nervous breakdown, coma and diarrhea when ingested over a certain amount of time and cause serious damage to the human body during ingestion. It is forbidden to use for infants under 7 years old and elderly people who are not able to have immunity. Therefore, there is a great concern about safety and side effects.
To remove bad breath, mouthwashes such as tooth brushing, tooth brushing, and gum chewing are used. Sulser and Tonzetich studied the mouthwashing effect of mouthwash, and Gilmore Bhaskar and Tonzetich found that tongue wiping was effective in decreasing halitosis. Morris and Read, Pianotti and Pitts have investigated the effect of oral disinfectant diluting solutions on halitosis, and Gaffars et al. Have reported on haloth- er reduction effects of placebo rinse, salted mouthrinse and zinc chloride saliva.
In particular, the causes of halitosis and plaque are caused by the causative organisms of Streptococcus mutans, Streptococcus sorbinus, Haemophilus actinomycetemcomitans.
Streptococcus mutans is a decubitus organism in the mouth, which decomposes saccharides and carbohydrates remaining on the tooth surface to produce lactic acid, which in turn erodes the enamel.
Streptococcus sorbinus is a major causative organism (caries-inducing bacteria) of dental caries such as Streptococcus mutans, Streptococcus sorbinus, Lactobacillus, It is also known as Actinomyces. Dental caries is a chronic disease of slow progression.
Haemophilus actinomycetemcomitans causes chronic marginal periodontitis.
As experimental condition for the above strain,
In the experiment of the strain of the present invention, 100 mg of a sample was sterilized and added to 500 μl of a BHIS medium to dissolve it. Each of the bacteria was inoculated and cultured in a 37 ° C. incubator for 12 hours, and then diluted appropriately in a BHIS solid medium Platelet counts were determined by counting the number of colonies appearing after culturing for one day.
The percentage of bacteria in the control group was determined as 100%, and expressed as Antibacterial activity (%).
The medium composition was as follows: BHIG: (Brain Heart Infusion, BHI) Agar powder 2%, Glucose 0.5% / BHIS: (Brain Heart Infusion, BHI) Agar powder 2% and Sucrose 0.5%.
Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.
In the present invention, 100 mg of a sample is sterilized, and then 500 ul of BHIS medium is added to dissolve. Each microorganism is inoculated and cultured in a 37-well incubator for 12 hours, and then plated on a BHIS solid medium. The number of colonies after the infection was determined and the number of viable cells was determined.
Antibacterial activity (%) was determined by comparing the percentage of viable bacteria with 100% of the plate bacteria in the comparative group.
In order to test the present invention, a sample group was formed as shown in Table 1 below.
BHIG: (Brain Heart Infusion, BHI) Agar powder 2%, Glucose 0.5%
BHIS: (Brain Heart Infusion, BHI) Agar powder 2%, Sucrose 0.5%
BHIG medium was used instead of BHIS in the medium.
The strain experiment of the present invention can be seen in the accompanying drawings which will be described below.
FIG. 1 shows antibacterial activity against Streptococcus mutans, which is a mixture of three natural products (sun salt, mint, and millennia). As a result, 86.4% of the undiluted solution was inhibited and the dilution ratio was high The tendency of the inhibition rate to be lowered is shown in the graph of Table 1 and FIG.
Fig. 2 shows the antimicrobial effect of Streptococcus mutans against the cavities of the three mixed liquids (sun-dried salt, green tea, laurel leaf). As a result, 88.5% inhibitory effect was obtained in the undiluted solution, And the inhibition rate was found to be lowered. The results are shown in Table 3 and FIG. 2 below.
Fig. 3 shows the antimicrobial effect of Streptococcus sorbinus on the nasal decongestant by the 5 mixture liquids (sun salt, green tea, laurel leaf, and mint). As a result, 86.7% The higher the drainage rate, the lower the inhibition rate was, and the results are shown in Table 4 and Fig.
FIG. 4 shows antibacterial activity against Streptococcus sorbinus, a mixture of three natural products (sun salt, mint, and millennium). As a result, it was found that 98.8% of the undiluted solution had an inhibitory effect, The inhibition rate tended to be lowered as shown in Table 5 and FIG. 4 below.
FIG. 5 shows the antimicrobial effect of Streptococcus sorbinus on three antimicrobial agents (sun salt, green tea, and laurel leaf). As a result, 99.9% inhibitory effect was obtained in the undiluted solution. And the inhibition rate was lowered, which can be seen in Table 6 and FIG.
FIG. 6 shows the antibacterial effect of Streptococcus sorbinus on the dentifrice with five mixed liquids (sun salt, green tea, laurel leaf, and mulberry) and showed an inhibitory effect of 98.5% And the inhibition rate was lowered as shown in Table 7 and FIG. 6.
FIG. 7 shows antibacterial activity against Haemophilus actinomycetemcomitans, which is a mixture of three natural products (sun salt, mint, and millennium). As a result, the inhibitory effect of the undiluted solution was 98.3% , And the inhibition rate tended to be lower as the dilution ratio was higher, as shown in Table 8 and FIG. 7 below.
FIG. 8 shows antibacterial effects of Haemophilus actinomycetemcomitans on periodontal fungus with three mixtures (sun salts, green tea, laurel leaves), showing 99.9% inhibition effect of undiluted solution , And that the higher the dilution factor, the lower the inhibition rate, which can be seen in Table 9 and FIG. 8 below.
FIG. 9 shows that the antimicrobial effect of Haemophilus actinomycetemcomitans was inhibited by the 5 mixture solutions (sun salt, green tea, laurel leaf, and mint), and the inhibitory effect of the undiluted solution was 96.9% , Which is shown in Table 10 and FIG. 9.
As can be seen from FIGS. 1 to 9, when the composition of the present invention is used as an oral cleansing agent, it can be used as an oral cleaning agent, such as a tranquilizer, a laurel water (Et), a laurel water (Dw) The activity of the Streptococcus mutans strain, Streptococcus sorbinus strain, Haemophilus actinomycetemcomitans strain was extinguished, and the efficacy of the laurel, .
Table 1 shows the antibacterial effect of the oral cleaning agent containing the extract of laet (La) and laurel (Dw) against the chronic periodontitis bacteria of the Haemophilus actinomycetemcomitans strain of Haemophilus actinomycetemcomitans.
Hereinafter, the results of the clinical experiment related to the saline-containing oral cleanser of the present invention will be described below
One. Experimental group And control group
Group A = oral cleansing group containing sun-salt (Experiment A group)
Group B = group using natural mouthwash (Experiment B group)
Control = distilled water use group (control group)
2. Statistics used in the experiment - SPSS Use
Each group comparison - one-way ANOVA
Point-by-point comparison - paired t-test
3. Participants in each group
Planned and recruited early experimental and control groups - 40 per group
Final experimental group and control group
Experiment group A - 25 (15 dropouts) (dropout rate 37.5%)
Experiment group A - 26 (14 dropouts) (dropout rate 35%)
Control group - 26 (14 dropouts) (dropout rate: 35%)
* The criterion for dropping out was that the toothbrushing or other oral cleaning agent was omitted during the experiment.
4. Experimental process
After 3 days of oral hygiene control was prohibited for experimental gingivitis formation, oral cleaning agent was applied to each group for 7 days.
3 days after the experiment, the baseline was used as the baseline. After 7 days (10 days from the total), the value was 1 W. The measured values were GI, PI, SBI, simple breath test and GCF ) Were performed.
As for the bleeding, color, and edema, which were not explained in the experimental plan, detailed statistics for measuring SBI and GI were used.
Also, the comparison of the amount of oral cleansing agent used for compliance evaluation is also only for reference.
5. Data
Table 12 Thread Tools Control gingivl index The figures are compared.
Table 13 Experimental group Control plaque index The figures are compared.
** (A, C)
Table 14 Experimental group Control sulcular bleeding index The figures are compared.
Table 15 Experimental group Control bad breath levels A comparison table .
** (B, C)
** (B, C)
Table 16 Experimental group Control GCF It is a comparison table of figures.
Table 17
Experimental group
Control
Baseline
And 1
week
Time average of the test results.
6. Answer the question
1) The number of people recruited for the first 40 people per each group, and the final data is as above. The final data were calculated only for the baseline and for all the subjects after one week.
2) Bleeding data are used only to show the frequency of the findings. In practice, it is recommended to refer to direct gingival inflammation indicators such as GI and SBI for meaningful data.
3) Significance test was performed at each time point between the experimental group, the experimental group and the control group, and the data between the baseline and the one-week time test in each experimental group and the control group.
4) The experiment period was changed to one week because of the prohibition of oral hygiene control for 5 days to form experimental gingivitis as initial experiment plan and the experimental period of 2 weeks - There was an objection to the ethical problem that could arise if it was said that
Therefore, we performed the minimum number of days for experimental gingivitis (3 days) and the minimum period of one week for evaluating the difference.
In addition, as can be seen from the plaque index, it is difficult to remove the plaque by only the oral cleaning agent. Therefore, when the test period is 2 weeks, more plaque accumulation occurs and the data may be erroneous due to increased inflammation due to plaque accumulation. Since the gingivitis is reduced within one week when brushing is performed, the period of observation for one week is considerably sufficient.
5) The results show good results, and the oral cleaning agent is a preventive agent for liver bad breath and gingivitis. However, the clinical result is not shown to be able to be improved as a therapeutic agent. There was no significant effect on the formation of stapes, so it should be explained at the presentation.
6) In the last Table 17, we found that in the experimental group (A, B), no increase in the odor was observed after one week, but in the control group, the odor was increased, Can be suppressed. In the GCF, there was a decrease in gingival crevicular fluid only in the experimental group A in the maxillary molar (# 16), but no decrease in the gingival crevicular fluid in the experimental group B and the control group. ) Showed a decrease in the gingival crevicular fluid and an increase in the gingival crevicular fluid was observed in the control group.
Note that an increase in gingival crevicular fluid indicates an increase in gingivitis. GI and SBI index indicating gingivitis were not increased after one week in experimental groups (A, B), but increased in control group.
In addition, the plaque index (PI) showed an increase in plaque index in the experimental group A, and the plaque index did not change in the experimental group B and the control group. That is, there is no plaque removal ability.
As described above, the oral cleaning agent using the natural product of the present invention can be produced by: 1) preparing an oral cleaning agent with an extract mixture of sun salt, chunghwa seeds and mint, 2) preparing an oral cleaning agent with an extract mixture of sun salt, green tea and laurel leaf, + Green tea + laurel leaf + mint extract mixture to provide mouthwash using an extract mixture of natural products. If necessary, firstly, the most suitable sun salt according to age is selected, Third, it replaces chemical substances, which are the components of oral cleaning agent, with natural products, contributing to consumers' trust and health promotion by replacing chemicals, which are components of oral cleaning agent, with natural products. And to increase the incomes of the poor.
Claims (8)
(a) preparing a natural mouthwash composition by selecting a sun salt, a milky water, a mint, a green tea, and a laurel leaf;
(b) preparing ethanolic extracts of ethanol from the freeze-dried powder of the perennial stalks, and extracting them for 9 hours;
(c) adding distilled water to each of the prepared mint leaves, green tea leaves, and laurel leaves, and extracting the extracts for 9 hours;
A method for preparing an oral cleaning agent using natural products, comprising: mixing sunflower and 5% ethanol extract, green tea extract, laurel water extract, mint extract, and boiling water to prepare a mouthwash detergent.
Wherein the powdered 500-milliliter of ethanol is extracted with 500 ml of ethanol for 9 hours to prepare an extract.
The mint leaf extract, green tea leaf extract, and laurel leaf extract were prepared by adding 300 ml of distilled water to 30 g of prepared mint leaves, green tea leaves, and laurel leaves, respectively, and extracting them for 9 hours to prepare mint leaf extract, green tea leaf extract, Wherein the method comprises the steps of:
A method for preparing an oral cleaning agent using natural products, comprising: mixing 2 g of the prepared sunflower salt, 400 쨉 l of green tea extract, and 100 쨉 l of a Laurel leaf extract and mixing the mixture with boiling water to prepare 100 ml of oral cleaning agent.
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
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CN105560129A (en) * | 2016-03-03 | 2016-05-11 | 陈毅忠 | Preparation method for purely natural herbal disinfectant wet wipe |
KR20180079959A (en) * | 2017-01-03 | 2018-07-11 | 가오 용리앙 | Mouthwash containing green tea |
KR102173528B1 (en) * | 2020-02-28 | 2020-11-03 | 주식회사 바른 | Ingestible Tablet or Powder Type Oral Cleaning Composition |
KR20210009583A (en) | 2019-07-17 | 2021-01-27 | 제너럴바이오(주) | COMPOSITION COMPRISING Machilus thunbergii FOR PREVENTING, IMPROVING OR TREATING ORAL DISEASE |
KR20210094945A (en) | 2020-01-22 | 2021-07-30 | 제너럴바이오(주) | COMPOSITION COMPRISING EXTRACT OF Chrysanthemum indicum FOR PREVENTING, ALLEVIATING OR TREATING ORAL DISEASE |
KR102312450B1 (en) | 2020-06-11 | 2021-10-13 | 제너럴바이오(주) | Composition for prevention or improvement of oral diseases containing ginseng fermented extract and Sophora flavescens extract as an active ingredient |
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2013
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Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105560129A (en) * | 2016-03-03 | 2016-05-11 | 陈毅忠 | Preparation method for purely natural herbal disinfectant wet wipe |
KR20180079959A (en) * | 2017-01-03 | 2018-07-11 | 가오 용리앙 | Mouthwash containing green tea |
KR20210009583A (en) | 2019-07-17 | 2021-01-27 | 제너럴바이오(주) | COMPOSITION COMPRISING Machilus thunbergii FOR PREVENTING, IMPROVING OR TREATING ORAL DISEASE |
KR20210094945A (en) | 2020-01-22 | 2021-07-30 | 제너럴바이오(주) | COMPOSITION COMPRISING EXTRACT OF Chrysanthemum indicum FOR PREVENTING, ALLEVIATING OR TREATING ORAL DISEASE |
KR102173528B1 (en) * | 2020-02-28 | 2020-11-03 | 주식회사 바른 | Ingestible Tablet or Powder Type Oral Cleaning Composition |
WO2021172692A1 (en) * | 2020-02-28 | 2021-09-02 | 주식회사 바른 | Ingestible tablet or powdered oral cleaning composition |
US11464715B2 (en) | 2020-02-28 | 2022-10-11 | Bareun Co., Ltd. | Ingestible tablet or powder type oral cleaning composition |
KR102312450B1 (en) | 2020-06-11 | 2021-10-13 | 제너럴바이오(주) | Composition for prevention or improvement of oral diseases containing ginseng fermented extract and Sophora flavescens extract as an active ingredient |
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