CN107362112B

CN107362112B - Gum-protecting and healing toothpaste

Info

Publication number: CN107362112B
Application number: CN201710635736.XA
Authority: CN
Inventors: 冯保国; 冯玮琳; 陈佳娜
Original assignee: Jiannan China Co ltd
Current assignee: Jiannan China Co ltd
Priority date: 2017-07-28
Filing date: 2017-07-28
Publication date: 2020-08-25
Anticipated expiration: 2037-07-28
Also published as: CN107362112A

Abstract

The invention discloses a gingival protection and healing toothpaste, which comprises cordyceps militaris essence, allantoin, tranexamic acid, O-cymene-5-ol, potassium nitrate and a red ball ginger extract. The gum protecting and healing toothpaste disclosed by the invention has obvious anti-inflammatory and antibacterial effects, also has the gum protecting effect of preventing gum bleeding and oral ulcer, can comprehensively prevent and solve various oral problems, and is suitable for people with oral problems of gum swelling and bleeding, gum inflammation, tooth allergy and stabbing pain and the like.

Description

Gum-protecting and healing toothpaste

Technical Field

The invention relates to toothpaste, in particular to toothpaste for protecting gingiva and healing gingiva.

Background

Gingivitis, periodontitis, tooth sensitivity, oral ulcer and the like are the most common oral diseases at present, and the importance of oral health care is also recognized by people. Gingivitis is characterized by bleeding, redness and swelling of the gums, swelling and pain, which continues to invade the hard tissues and produce periodontitis, including inflammation of the gum tissues and manifestations of systemic diseases in the gums. Most of the bleeding of gum is because the soft scale of the bacterial plaque (bacteria) on the tooth surface is not removed in time, calcium ions in saliva calcify the bacterial plaque to slowly form tartar, the gum of the corresponding part is stimulated by the tartar for a long time, the capillary vessel expands, the permeability is enhanced, the blood flow is increased, so that the gum becomes bright red or dark red, the gum nipple swells, the point color disappears, the edge becomes thick, the gum is not attached to the tooth surface any more, the gum is soft and fragile, and the gingival sulcus deepens. When brushing teeth or eating food, the gum in such an inflammatory state is easily bleedings due to mechanical stimulation. It is known that various oral diseases are caused by bacteria in the oral cavity, and in order to prevent or treat oral diseases, the environment of oral flora should be improved as a whole from the root problems, starting from the antibacterial and anti-inflammatory effects.

Disclosure of Invention

The invention aims to overcome the defects in the prior art and provide the gingiva protecting and healing toothpaste.

In order to achieve the purpose, the invention adopts the technical scheme that: a toothpaste for protecting gingiva and healing wound comprises scarlet caterpiller fungus essence, allantoin, tranexamic acid, O-cymene-5-ol, potassium nitrate and Zingiber zerumbet extract; the Cordyceps militaris essence is a mixture of Cordyceps militaris extract and Cordyceps militaris fruiting body.

The cordyceps militaris is edible fungus for both food and medicine, and the cordyceps militaris extract can be purchased in the market and can also be a refined extract obtained by carrying out water extraction and alcohol precipitation on cordyceps militaris fruiting bodies. The Cordyceps militaris fruiting body is obtained by inoculating Cordyceps militaris strain on solid culture medium, culturing for a certain period of time, developing and differentiating mycelium to form orange rod-shaped stroma (fruiting body), collecting fresh fruiting body, air drying, and grinding; the fruiting body powder is refined after water extraction and alcohol precipitation, and effective components such as cordycepin, adenosine, cordycepic acid, polysaccharide, SOD enzyme and the like are efficiently concentrated, especially the effective components of cordycepin.

The cordyceps militaris sporocarp provided by the invention is composed of substances such as water, ash, protein and fat, contains various volatile compounds, contains more aromatic aldehyde substances, and has certain antibacterial and anti-inflammatory effects, but the aromatic volatile components cannot be retained for more than one hour at the extraction temperature of 50 ℃. Therefore, the effective components in the cordyceps militaris sporocarp can not be absolutely reserved in the refining, heating and purifying process, so that the volatile components of the cordyceps militaris sporocarp can completely enter a toothpaste system by adding the cordyceps militaris sporocarp.

Cordycepin, the first nucleoside antibiotic separated from fungi, has a molecular structure of 3-deoxyadenosine, has a broad-spectrum antibacterial effect, and is found to be capable of inhibiting the growth of pathogenic bacteria such as streptococcus, staphylococcus and the like, and has strong antiviral activity, so that the cordycepin has natural antibacterial, anti-inflammatory and antiviral effects in a toothpaste formula. Because the cordycepin is the main antibacterial component in the cordyceps militaris extract, the content of the cordycepin in the toothpaste formula is directly influenced by the addition amount of the cordyceps militaris extract. The invention uses the cordyceps militaris sporocarp and the cordyceps militaris extract, so that the system contains various functional components such as cordycepin, cordycepic acid, polysaccharide, SOD enzyme and the like, and also contains the volatile components of the cordyceps militaris sporocarp per se, thereby the gum and tooth protecting effects are exerted to the best.

Allantoin exists in natural plants such as Chinese yam, beet and wisteria, and has the functions of repairing epithelial tissue, promoting skin ulcer and wound healing. Allantoin is used in toothpaste system for antibacterial, antiulcer, and antioxidant effects in daily oral care, and is available in commercial products, such as Merck

Allantoin, and the like.

Tranexamic Acid, also known as Tranexamic Acid, is applied to various clinical traumatic bleeding symptoms, can inhibit the action of plasmin, has good hemostatic and anti-inflammatory effects, and can be selected from commercially available products such as Tranexamic Acid of Nanjing Babyaceae Biochemical industry Co.

O-cymene-5-alcohol is a broad-spectrum bactericide, has strong penetration sterilization effect, has good antibacterial effect on staphylococcus aureus, porphyromonas gingivalis and oral pathogenic bacteria, can penetrate into dental plaque to sterilize, is suitable for inflammation patients of gingivitis, periodontitis and the like, and can select commercially available products, such as Isopropyl methyl phenol of Nanjing Sbyaceae Biochemical industry Limited company.

Potassium nitrate, a desensitizing active ingredient in the United states Food and Drug Administration (FDA) monograph for tooth desensitization products, releases potassium ions, prevents the nerve endings of the dentinal tubules from being activated, and at sufficient potassium ion concentrations, prevents the nerve from being stimulated by the compressed dentin, reduces the permeability of the dentin, and thereby slows down dentin hypersensitivity.

The red ball ginger is distributed in China Hainan, Yunnan, Guangxi, India, Malaysia, Indonesia and the like, and is a perennial root herbaceous plant. The Zingiber zerumbet Smith can be used for relieving inflammation and pain, and contains sesquiterpene and flavonoid as main active ingredients, especially zerumbone in ketone, which has effect in relieving toothache. The Extract of Zingiber Zerumbet of the present invention can be selected from commercially available products, such as Zingiber Zerumbet Extract from Croda, etc.

When the cordyceps militaris essence, allantoin, tranexamic acid, O-cymene-5-ol, potassium nitrate and the zingiber zerumbet extract are applied to the gum-protecting and healing toothpaste, the effects of compounding and synergism can be achieved, the effects of stopping bleeding, protecting gums and soothing allergy are achieved, and meanwhile, the overall anti-inflammation and anti-bacteria effects of the toothpaste formula are greatly enhanced.

Preferably, the gum-protecting and healing toothpaste comprises the following preparation raw materials in percentage by weight: 0.1-5% of cordyceps militaris essence, 0.1-2% of allantoin, 0.05-0.1% of tranexamic acid, 0.01-0.1% of O-cymene-5-alcohol, 3-10% of potassium nitrate and 0.1-1% of Zingiber zerumbet extract.

The cordyceps militaris essence has a good antibacterial and anti-inflammatory effect, and the antibacterial effect is enhanced along with the increase of the content of the cordyceps militaris essence. Repeated research by the inventor shows that when the content of the cordyceps militaris essence is lower than 0.1%, the antibacterial effect of the cordyceps militaris essence in the toothpaste is not significant, but when the content of the cordyceps militaris essence is higher than 5%, the antibacterial effect is not significantly increased along with the increase of the addition amount of the cordyceps militaris essence, the production cost and the antibacterial effect are integrated, and when the content of the cordyceps militaris essence is 0.1-5%, the cordyceps militaris essence has a good antibacterial effect; when the components are matched with each other at the ratio, the inflammation diminishing and bacteriostasis effects are obviously enhanced, and the problem of redness and swelling and bleeding of the gum can be effectively relieved.

Preferably, the gum-protecting and healing toothpaste comprises the following preparation raw materials in percentage by weight: 0.5-3% of cordyceps militaris essence, 0.2-0.8% of allantoin, 0.05-0.08% of tranexamic acid, 0.05-0.08% of O-cymene-5-alcohol, 3-5% of potassium nitrate and 0.1-0.5% of Zingiber zerumbet extract.

The inventor repeatedly researches to obtain that the optimal anti-inflammatory and antibacterial effects and the overall taste of the toothpaste are achieved when the contents of the components are the above contents, and the problem of redness, swelling and bleeding of gum is obviously improved.

Preferably, the weight ratio of the cordyceps militaris extract to the cordyceps militaris sporocarp is as follows: cordyceps militaris extract: and (3) cordyceps militaris fruit body 80-99.9: 0.01 to 20.

When the cordyceps militaris extract and the cordyceps militaris sporocarp are in the weight ratio, the cordyceps militaris extract and the cordyceps militaris sporocarp can fully exert respective effects, so that a system contains cordycepin, cordycepic acid, polysaccharide, SOD enzyme and various volatile compounds, and the effects of gum and tooth protection are achieved.

More preferably, the weight ratio of the cordyceps militaris extract to the cordyceps militaris sporocarp is as follows: cordyceps militaris extract: and (3) cordyceps militaris fruit body of 85-92: 8-15.

When the cordyceps militaris extract and the cordyceps militaris sporocarp are in the weight ratio, the toothpaste achieves the best sterilization effect.

Preferably, the raw materials for preparing the gingival protection and healing toothpaste further comprise a humectant, an abrasive, a thickening agent and a foaming agent.

Preferably, in the preparation raw materials of the gum-protecting and healing toothpaste, the weight percentage of the humectant is 40-60%, the weight percentage of the abrasive is 14-30%, the weight percentage of the thickener is 0.5-3%, and the weight percentage of the foaming agent is 1.8-3%.

Preferably, the foaming agent is a mixture of sodium lauryl sulfate and at least one of an amino acid surfactant and an amphoteric surfactant.

More preferably, the foaming agent is a mixture of sodium lauryl sarcosinate, sodium lauroyl methyltaurate and cocamidopropyl betaine with sodium lauryl sulfate.

When the foaming agent only uses the sodium dodecyl sulfate, the foaming agent has less and sparse foam and slightly poor mouth feel during tooth brushing, and when the foaming agent is matched with at least one of the amino acid surfactant and the amphoteric surfactant, the foaming speed can be increased, the foam is fine and rich, and the problem of dry and astringent mouth feel without foam is solved. The foaming agents are all selected from commercially available products, for example, sodium lauroyl sarcosinate can be selected from OLI-4208 of Olympic, sodium lauroyl methyltaurate can be selected from OLI-4901 of Olympic, cocamidopropyl betaine can be selected from CAPB DC of Galaxy Surfactants, and Lenotain C30 of Olympic, of which CAPB DC is particularly suitable.

Preferably, the abrasive is at least one of precipitated silica, ultrafine silica, calcium hydrogen phosphate, and calcium carbonate.

More preferably, the abrasive is a mixture of at least one of precipitated silica, calcium hydrogen phosphate and calcium carbonate and superfine silica, wherein the superfine silica accounts for 2-6 wt% of the raw materials for preparing the gingival protection and healing toothpaste.

The weight percentage of the superfine silicon dioxide in the preparation raw materials of the toothpaste is 2-6%. When the dosage of the superfine silicon dioxide is less than 2 percent, the surface of the paste body is not smooth, the mouth feel of tooth brushing is poor, and when the dosage of the superfine silicon dioxide is more than 6 percent, the paste body is easy to harden and go out of water at high temperature, so that the stability is influenced. The amount of the ultrafine silica absorbed is 30 to 44ml/20g, preferably 30 to 38ml/20g, and commercially available products such as general silica from the company Town can be used. The amount of the precipitated silica sucked is 15 to 25ml/20g, preferably 18 to 22ml/20g, and commercially available products such as Zeodent115 from HUBER can be used. The amount of calcium hydrogen carbonate absorbed is 5.0 to 6.5mL/20g, the apparent density is 0.80 to 1.00g/mL, the amount of absorbed liquid is preferably maximized and the apparent density is minimized, and commercially available products such as toothpaste grade calcium hydrogen phosphate from Depont may be used. The amount of calcium carbonate absorbed is 3.8 to 5.0ml/20g, preferably 3.8 to 4.2ml/20g, and commercially available products such as calcite powder from the company Mintellian can be used.

Preferably, the thickener is at least one of sodium carboxymethylcellulose, carbomer and xanthan gum; the humectant is at least one of sorbitol, glycerol, polyethylene glycol and propylene glycol.

The sodium carboxymethylcellulose is commercially available, such as TH10 (toothpaste grade) from ASHLAND, and has a substitution degree of 0.75-1.02, preferably 0.95-1.02, and a 2% aqueous solution viscosity of 600-3000 mPas, preferably 1200-2500 mPas. Carbomers can be selected from the commercial products, for example from the Lubrizol company

POLYMER (toothpaste grade), adjusted to pH around 7 (within the range of 6.5-7.5) with 25% NaOH solution, measured at 20rpm with Brookfield RV6 spindle, viscosity of 0.6% neutralized aqueous solution: 15000 to 40000 mPas, preferably 20000 to 30000 mPas. Xanthan gum, also known as xanthan gum, is commercially available, e.g. from CPKelco

Xanthan Gum (oral grade) with a viscosity of 1200-1600 mPas measured in a 1% KCl solution at 20rpm with a Brookfield RV6 rotor.

In addition to the specific requirements of the components, other auxiliary materials can be added or used within the range of not damaging the effect of the invention, the auxiliary materials can be sweetening agents, pH regulators, spices, coloring agents, fluoride and the like, and the ordinary content can be selected by the skilled person according to the conventional standard.

Preferably, the gum-protecting healing toothpaste further comprises the following components in percentage by mass: 0.1-2% of sweetening agent, 0.1-3% of pH regulator and 0.1-2% of spice.

Preferably, the sweetener is at least one of sodium saccharin, sucralose, and stevia; the spice is at least one of menthol, peppermint oil, spearmint oil, wintergreen oil, peppermint, sweet orange oil, lemon oil, anise oil and eucalyptus oil; the pH regulator is at least one of sodium hydroxide, anhydrous trisodium phosphate, sodium tripolyphosphate, alkaline phosphate and sodium dihydrogen phosphate.

The preparation method of the gingival protection and healing toothpaste comprises the following steps:

(1) pre-dissolving potassium nitrate and auxiliary materials;

(2) premixing the friction agent and the thickening agent;

(3) adding the humectant and the materials in the step (1) into a paste preparation tank;

(4) opening a vacuum system of the paste making tank, vacuumizing, adding the mixture obtained in the step (2), cordyceps militaris essence, allantoin, tranexamic acid, O-cymene-5-alcohol and a red ball ginger extract, and stirring and homogenizing under the vacuum condition of-0.05 Mpa to-0.085 Mpa;

(5) adding foaming agent into the paste preparation tank, keeping the vacuum between-0.05 Mpa and-0.085 Mpa, and continuously stirring and homogenizing;

(6) degassing a paste preparation tank until the vacuum degree is-0.092 Mpa to-0.098 Mpa, and discharging paste to obtain the gum-protecting and healing toothpaste.

The invention has the beneficial effects that: the gum protecting and healing toothpaste provided by the invention has remarkable anti-inflammatory and antibacterial effects, also has a gum protecting effect of preventing gum bleeding and dental ulcer, can comprehensively prevent and solve various oral problems, and is suitable for people with oral problems of gum swelling and bleeding, gum inflammation, tooth allergy and stabbing pain and the like.

Drawings

FIG. 1 is a comparison graph of the bacteriostasis test of the toothpastes of example 5 and comparative examples 1-5 on staphylococcus aureus;

FIG. 2 is a comparison graph of the bacteriostatic tests of the toothpastes in example 5 and comparative examples 1-5 on Streptococcus mutans;

FIG. 3 is a comparison chart of the bacteriostasis test of the toothpastes of example 5 and comparative examples 1-5 on the porphyromonas gingivalis.

Detailed Description

To better illustrate the objects, aspects and advantages of the present invention, the present invention will be further described with reference to specific examples.

Example 1

One embodiment of the gum-protecting and healing toothpaste comprises the following preparation raw materials in percentage by weight: 0.1% of cordyceps militaris essence, 0.1% of allantoin, 0.05% of tranexamic acid, 0.05% of O-cymene-5-alcohol, 3% of potassium nitrate, 0.1% of red-ball ginger extract, 40% of humectant, 14% of abrasive, 0.5% of thickening agent, 1.8% of foaming agent, 0.1% of sweetening agent, 0.1% of pH regulator, 0.1% of perfume and the balance of deionized water;

the cordyceps militaris essence is a mixture of cordyceps militaris extract and cordyceps militaris fruiting body, and the weight ratio of the cordyceps militaris extract to the cordyceps militaris fruiting body is as follows: cordyceps militaris extract: cordyceps militaris fruit body 99.9: 0.01; the humectant is sorbitol; the thickening agent is sodium carboxymethyl cellulose; the foaming agent is a mixture of sodium lauroyl sarcosinate and sodium dodecyl sulfate; the mixture of the abrasive precipitated silica and the superfine silica is prepared, wherein the weight percentage of the superfine silica in the raw materials for preparing the gum-protecting and healing toothpaste is 2%; the sweetener is saccharin sodium; the spice is menthol; the pH regulator is alkaline phosphate and sodium dihydrogen phosphate.

Example 2

One embodiment of the gum-protecting and healing toothpaste comprises the following preparation raw materials in percentage by weight: 5% of cordyceps militaris essence, 2% of allantoin, 0.1% of tranexamic acid, 0.1% of O-cymene-5-alcohol, 10% of potassium nitrate, 1% of red globe ginger extract, 40% of humectant, 20% of abrasive, 3% of thickener, 3% of foaming agent, 2% of sweetener, 3% of pH regulator, 2% of perfume and the balance of deionized water;

the cordyceps militaris essence is a mixture of cordyceps militaris extract and cordyceps militaris fruiting body, and the weight ratio of the cordyceps militaris extract to the cordyceps militaris fruiting body is as follows: cordyceps militaris extract: cordyceps militaris fruit body 80: 20; the humectant is a mixture of sorbitol and glycerol; the thickening agent is a mixture of sodium carboxymethylcellulose and carbomer; the foaming agent is a mixture of sodium lauroyl methyl taurate and sodium dodecyl sulfate; the friction agent is a mixture of calcium hydrophosphate and superfine silicon dioxide, and the weight percentage of the superfine silicon dioxide in the raw materials for preparing the gum-protecting and healing toothpaste is 6%; the sweetener is sucralose; the spice is spearmint oil; the pH regulator is sodium hydroxide.

Example 3

One embodiment of the gum-protecting and healing toothpaste comprises the following preparation raw materials in percentage by weight: 0.5% of cordyceps militaris essence, 0.2% of allantoin, 0.05% of tranexamic acid, 0.01% of O-cymene-5-alcohol, 3% of potassium nitrate, 0.1% of red-ball ginger extract, 60% of humectant, 30% of abrasive, 0.5% of thickening agent, 1.8% of foaming agent, 0.1% of sweetening agent, 0.1% of pH regulator, 0.1% of perfume and the balance of deionized water;

the cordyceps militaris essence is a mixture of cordyceps militaris extract and cordyceps militaris fruiting body, and the weight ratio of the cordyceps militaris extract to the cordyceps militaris fruiting body is as follows: cordyceps militaris extract: cordyceps militaris fruit body 92: 8; the humectant is a mixture of sorbitol and propylene glycol; the thickening agent is a mixture of sodium carboxymethylcellulose and xanthan gum; the foaming agent is a mixture of cocamidopropyl betaine and sodium lauryl sulfate; the friction agent is a mixture of calcium carbonate and superfine silicon dioxide, and the weight percentage of the superfine silicon dioxide in the raw materials for preparing the gum-protecting and healing toothpaste is 4%; the sweetener is stevia rebaudiana; the spice is lemon oil; the pH regulator is anhydrous trisodium phosphate.

Example 4

One embodiment of the gum-protecting and healing toothpaste comprises the following preparation raw materials in percentage by weight: 3% of cordyceps militaris essence, 0.8% of allantoin, 0.08% of tranexamic acid, 0.08% of O-cymene-5-alcohol, 4% of potassium nitrate, 0.5% of Zingiber zerumbet extract, 45% of humectant, 20% of abrasive, 2% of thickening agent, 2% of foaming agent, 1% of sweetener, 2% of pH regulator, 1% of perfume and the balance of deionized water;

the cordyceps militaris essence is a mixture of cordyceps militaris extract and cordyceps militaris fruiting body, and the weight ratio of the cordyceps militaris extract to the cordyceps militaris fruiting body is as follows: cordyceps militaris extract: cordyceps militaris fruit body 85: 15; the humectant is a mixture of sorbitol and glycerol; the thickening agent is carbomer; the foaming agent is a mixture of sodium lauroyl sarcosinate, sodium lauroyl methyltaurate and sodium dodecyl sulfate; the abrasive is a mixture of precipitated silicon dioxide and superfine silicon dioxide, and the weight percentage of the superfine silicon dioxide in the raw materials for preparing the gum-protecting and healing toothpaste is 6%; the sweetener is saccharin sodium; the spice is sweet orange oil; the pH regulator is anhydrous trisodium phosphate.

Example 5

One embodiment of the gum-protecting and healing toothpaste comprises the following preparation raw materials in percentage by weight: 3% of cordyceps militaris essence, 0.1% of allantoin, 0.1% of tranexamic acid, 0.05% of O-cymene-5-alcohol, 5% of potassium nitrate, 0.1% of Zingiber zerumbet extract, 50% of humectant, 22% of abrasive, 2% of thickening agent, 2% of foaming agent, 1% of sweetener, 2% of pH regulator, 1% of perfume and the balance of deionized water;

the cordyceps militaris essence is a mixture of cordyceps militaris extract and cordyceps militaris fruiting body, and the weight ratio of the cordyceps militaris extract to the cordyceps militaris fruiting body is as follows: cordyceps militaris extract: cordyceps militaris fruit body 90: 10; the humectant is a mixture of sorbitol and glycerol; the thickening agent is carbomer; the foaming agent is a mixture of sodium lauroyl sarcosinate, sodium lauroyl methyltaurate and sodium dodecyl sulfate; the abrasive is a mixture of precipitated silicon dioxide and superfine silicon dioxide, and the weight percentage of the superfine silicon dioxide in the raw materials for preparing the gum-protecting and healing toothpaste is 4%; the sweetener is saccharin sodium; the spice is sweet orange oil; the pH regulator is anhydrous trisodium phosphate.

Example 6

According to an embodiment of the gum-protecting healing toothpaste, the gum-protecting healing toothpaste of the embodiment is different from the gum-protecting healing toothpaste of the embodiment 5 only in content of cordyceps militaris essence, and the cordyceps militaris essence of the gum-protecting healing toothpaste of the embodiment accounts for 5% by weight of a raw material for preparing the toothpaste.

Example 7

According to an embodiment of the gum-protecting healing toothpaste, the gum-protecting healing toothpaste of the embodiment is different from the gum-protecting healing toothpaste of the embodiment 5 only in content of cordyceps militaris essence, and the cordyceps militaris essence of the gum-protecting healing toothpaste of the embodiment accounts for 4% by weight of a raw material for preparing the toothpaste.

Example 8

According to an embodiment of the gum-protecting healing toothpaste, the gum-protecting healing toothpaste of the embodiment is different from the gum-protecting healing toothpaste of the embodiment 5 only in content of cordyceps militaris essence, and the cordyceps militaris essence of the gum-protecting healing toothpaste of the embodiment accounts for 2% by weight of a raw material for preparing the toothpaste.

Example 9

According to an embodiment of the gum-protecting healing toothpaste, the gum-protecting healing toothpaste of the embodiment is different from the gum-protecting healing toothpaste of the embodiment 5 only in content of cordyceps militaris essence, and the cordyceps militaris essence of the gum-protecting healing toothpaste of the embodiment accounts for 1% by weight of a raw material for preparing the toothpaste.

Example 10

According to an embodiment of the gum-protecting healing toothpaste, the gum-protecting healing toothpaste of the embodiment is different from the gum-protecting healing toothpaste of the embodiment 5 only in content of cordyceps militaris essence, and the content of the cordyceps militaris essence in the raw materials for preparing the toothpaste is 0.1% by weight.

Example 11

An embodiment of the gingival protection and healing toothpaste provided by the invention is different from embodiment 5 only in the weight ratio of cordyceps militaris extract to cordyceps militaris fruit body, and the weight ratio of cordyceps militaris extract to cordyceps militaris fruit body is as follows: cordyceps militaris extract: cordyceps militaris fruit body 99.9: 0.01.

example 12

An embodiment of the gingival protection and healing toothpaste provided by the invention is different from embodiment 5 only in the weight ratio of cordyceps militaris extract to cordyceps militaris fruit body, and the weight ratio of cordyceps militaris extract to cordyceps militaris fruit body is as follows: cordyceps militaris extract: cordyceps militaris fruit body 95: 5.

example 13

An embodiment of the gingival protection and healing toothpaste provided by the invention is different from embodiment 5 only in the weight ratio of cordyceps militaris extract to cordyceps militaris fruit body, and the weight ratio of cordyceps militaris extract to cordyceps militaris fruit body is as follows: cordyceps militaris extract: cordyceps militaris fruit body 92: 8.

example 14

An embodiment of the gingival protection and healing toothpaste provided by the invention is different from embodiment 5 only in the weight ratio of cordyceps militaris extract to cordyceps militaris fruit body, and the weight ratio of cordyceps militaris extract to cordyceps militaris fruit body is as follows: cordyceps militaris extract: cordyceps militaris fruit body 85: 15.

example 15

An embodiment of the gingival protection and healing toothpaste provided by the invention is different from embodiment 5 only in the weight ratio of cordyceps militaris extract to cordyceps militaris fruit body, and the weight ratio of cordyceps militaris extract to cordyceps militaris fruit body is as follows: cordyceps militaris extract: cordyceps militaris fruit body 80: 20.

comparative example 1

The preparation raw materials of the toothpaste of the comparative example are different from those of the toothpaste of the example 5 in that the preparation raw materials of the toothpaste of the comparative example do not contain cordyceps militaris essence, allantoin, tranexamic acid, O-cymene-5-alcohol and a red globe ginger extract, namely the toothpaste of the comparative example comprises the following preparation raw materials in percentage by weight: 5% of potassium nitrate, 50% of a humectant, 22% of an abrasive, 2% of a thickening agent, 2% of a foaming agent, 1% of a sweetening agent, 2% of a pH regulator, 1% of a perfume and the balance of deionized water.

Comparative example 2

The preparation raw materials of the toothpaste of the comparative example are different from those of the toothpaste of the example 5 in that the preparation raw materials of the toothpaste of the comparative example do not contain cordyceps militaris essence, zingiber zerumbet hance extract and O-cymene-5-ol, namely the toothpaste of the comparative example comprises the following preparation raw materials in percentage by weight: allantoin 0.1%, tranexamic acid 0.1%, potassium nitrate 5%, humectant 50%, abrasive 22%, thickening agent 2%, foaming agent 2%, sweetening agent 1%, pH regulator 2%, perfume 1% and the balance of deionized water.

Comparative example 3

Compared with the preparation raw materials of the toothpaste in the example 5, the preparation raw materials of the toothpaste in the comparative example do not contain allantoin, tranexamic acid, O-cymene-5-alcohol and a red ball ginger extract, namely the toothpaste in the comparative example comprises the following preparation raw materials in percentage by weight: 3% of cordyceps militaris essence, 5% of potassium nitrate, 0.1% of zingiber zerumbet extract, 50% of humectant, 22% of abrasive, 2% of thickening agent, 2% of foaming agent, 1% of sweetener, 2% of pH regulator, 1% of perfume and the balance of deionized water.

Comparative example 4

The preparation raw materials of the toothpaste of the comparative example are different from those of the toothpaste of the example 5 in that the preparation raw materials of the toothpaste of the comparative example do not contain cordyceps militaris essence, allantoin, tranexamic acid and O-cymene-5-ol, namely the toothpaste of the comparative example comprises the following preparation raw materials in percentage by weight: 5% of potassium nitrate, 0.1% of Zingiber zerumbet Hance extract, 50% of humectant, 22% of abrasive, 2% of thickening agent, 2% of foaming agent, 1% of sweetening agent, 2% of pH regulator, 1% of perfume and the balance of deionized water.

Comparative example 5

The preparation raw materials of the toothpaste of the comparative example are different from those of the toothpaste of the example 5 in that the preparation raw materials of the toothpaste of the comparative example do not contain cordyceps militaris essence, allantoin, tranexamic acid and red globe ginger extract, namely the toothpaste of the comparative example comprises the following preparation raw materials in percentage by weight: 0.05% of O-cymene-5-alcohol, 5% of potassium nitrate, 50% of a humectant, 22% of an abrasive, 2% of a thickening agent, 2% of a foaming agent, 1% of a sweetener, 2% of a pH regulator, 1% of a perfume and the balance of deionized water.

Comparative example 6

The preparation raw materials of the toothpaste of the comparative example are different from those of the toothpaste of the example 5 only in the content of cordyceps militaris essence, and the cordyceps militaris essence of the toothpaste of the comparative example accounts for 6 wt% of the raw materials for preparing the toothpaste.

Comparative example 7

The preparation raw materials of the toothpaste of the comparative example are different from those of the toothpaste of the example 5 only in the weight ratio of the cordyceps militaris extract to the cordyceps militaris sporocarp, and the weight ratio of the cordyceps militaris extract to the cordyceps militaris sporocarp of the comparative example is as follows: cordyceps militaris extract: the ratio of cordyceps militaris fruit body is 100: 0.

Comparative example 8

The preparation raw materials of the toothpaste of the comparative example are different from those of the toothpaste of the example 5 only in the weight ratio of the cordyceps militaris extract to the cordyceps militaris sporocarp, and the weight ratio of the cordyceps militaris extract to the cordyceps militaris sporocarp of the comparative example is as follows: cordyceps militaris extract: the ratio of cordyceps militaris fruit body is 75: 25.

Example 16

The gum protecting and healing toothpaste disclosed in examples 1-15 and the toothpaste disclosed in comparative examples 1-8 are subjected to bacteriostasis tests on several common bacteria in the oral cavity.

The bacteriostasis test method comprises the following steps: the test was carried out by a third party agency according to the test method for resistance (inhibition) of Disinfection Specification 2.1.8.

Preparing the bacteriostatic tablets: preparing bacteriostatic agent raw paste sheets with the diameter of 5mm and the thickness of no more than 4mm in a clean sterile plate, and grouping every 4 sheets (blocks).

Preparation of negative pair photographs: taking a sterile dry filter paper sheet, and dripping sterile distilled water into each sheet for later use.

Inoculation of test bacteria by dipping with sterile cotton swab at 5 × 10 concentration⁵cfu/ml～5×10⁶Test bacterial suspension cfu/ml on nutrient agar medium plateThe surface is uniformly smeared for 3 times, the flat plate should be rotated for 60 degrees every time the surface is smeared for 1 time, finally the cotton swab is smeared for a circle around the edge of the flat plate, the flat plate is covered, and the cotton swab is placed at room temperature for drying for 5 min.

Sticking bacteriostatic agent sample pieces: each test is stuck with 1 infectious bacterium plate, each plate is stuck with 4 test sample plates of the same test group, 1 negative pair photo, and 5 negative pair photos. A sample was taken with sterile forceps and placed on the surface of the plate. The distance between the centers of the various pieces is more than 25mm, and the distance between the centers of the various pieces and the periphery of the flat plate is more than 15 mm. After the sample is placed, the sample is lightly pressed by using sterile tweezers to be tightly attached to the surface of the flat plate. The plate is covered, the plate is placed in an incubator at 37 ℃, and the result is observed after the plate is cultured for 16 to 18 hours. The direct measurement of the zone of inhibition was measured with a vernier caliper and recorded, and the test was repeated 3 times.

The strains tested at this time selected common oral pathogens: staphylococcus aureus, streptococcus mutans, and porphyromonas gingivalis.

And (3) bacteriostatic test evaluation standard: if the diameter of the bacteriostatic ring is larger than 7mm, the bacteriostatic ring is judged to have bacteriostatic action; if the diameter of the antibacterial ring is less than or equal to 7mm, the product is judged to have no antibacterial effect. The results of the bacteriostatic tests of the toothpastes in the examples 1 to 10 and the comparative examples 1 to 6 are shown in table 1, the results of the bacteriostatic tests of the toothpastes in the example 5 and the comparative examples 1 to 5 are shown in attached figures 1 to 3, and the results of the bacteriostatic tests of the toothpastes in the examples 5, 11 to 15 and the comparative examples 7 and 8 are shown in table 2.

TABLE 1 results of bacteriostatic tests on toothpastes described in examples 1 to 10 and comparative examples 1 to 6

As can be seen from table 1, the antibacterial effect of the gingival protection and healing toothpaste described in examples 1 to 10 on staphylococcus aureus, streptococcus mutans and porphyromonas gingivalis is superior to that of the toothpaste described in comparative examples 1 to 6, especially, as can be seen from comparison of the antibacterial effect of examples 5 and comparative examples 1 to 5 in fig. 1 to 3, the antibacterial effect of the toothpaste described in example 5 is greater than that of the toothpaste prepared by adding one or more of allantoin, tranexamic acid, cordyceps militaris essence, potassium nitrate, O-cymene-5-ol and zingiber zerumbet extract in comparative examples 1 to 5, which shows that the compounding of the allantoin, tranexamic acid, cordyceps militaris essence, potassium nitrate, O-cymene-5-ol and the zingiber zerumbet extract in a certain proportion range can play a synergistic role. The antibacterial effect comparative examples of the embodiment 5-10 and the comparative example 6 show that the antibacterial effect is increased along with the increase of the addition amount of the cordyceps militaris essence, but the antibacterial effect is not increased significantly along with the increase of the addition amount after the weight percentage content of the cordyceps militaris essence in the toothpaste preparation raw material is more than 5%.

TABLE 2 results of bacteriostatic tests on the toothpastes of examples 5, 11-15 and comparative examples 7, 8

As can be seen from table 2, under the condition of a certain content of the essence of cordyceps militaris, the antibacterial effect of the prepared toothpaste tends to increase and decrease with the increase of the content of the fruiting body of cordyceps militaris, and the weight ratio of the extract of cordyceps militaris to the fruiting body of cordyceps militaris is as follows: cordyceps militaris extract: and (3) cordyceps militaris fruit body of 85-92: the best effect is achieved when the temperature is 8-15 ℃.

Example 17

Clinical tests are carried out on the gum-protecting and healing toothpaste and the toothpastes in the comparative examples 1-5, and the specific test scheme is as follows:

first, test scheme

A random double-blind control test design is characterized in that 139 volunteer subjects suffering from gingival red swelling and bleeding problems of different degrees are collected, the ages of the volunteer subjects are 15-58 years old, the volunteer subjects are randomly divided into a test group and control groups I, II, III, IV and V, wherein the test group comprises 25 persons, the control group comprises 23 persons, the control group comprises II 23 persons, the control group comprises III 24 persons, the control group comprises IV 23 persons and the control group comprises V21 persons, the test group and the control groups comprise I, II, III, IV and V, the toothpaste adopting the gum-protecting healing toothpaste and the toothpaste adopting the comparative examples 1-5 are respectively used, and related indexes of teeth and gums of the volunteer subjects using the toothpaste are checked by adopting an oral epidemiology investigation method when the toothpaste is used for 4 weeks and 8 weeks.

Second, use method

Toothbrush: a soft-filament toothbrush;

dosage: the test subjects and the control subjects I-V in the test group and the control group brush teeth 2 times in the morning and at the evening every day, and 2 g of toothpaste is used each time.

Third, observe the item and index

1. Gingival Index (GI)

The conditions of 4 areas of the gingival margin at the mesial, distal, buccal and lingual sides are inspected, and the standard is 0-3 grade, and the standard is 0 grade (the gingiva is normal); 1 point (slight inflammation of gingiva, slight change of color, mild edema, no bleeding in probing); 2 points (moderate inflammation of gingiva, red, edema, brightness, probing bleeding); and 3 points (obvious gingival inflammation, red swelling, ulcer and tendency of automatic bleeding).

2. Gingival Sulcus Bleeding Index (SBI)

The gingival margin is inspected in 4 areas of the mesial, distal, buccal and lingual sides and classified into 4 grades and 1 (no bleeding); 2 points (bleeding when the gingival sulcus is slightly explored, the appearance of the gingiva is normal); 3 points (bleeding when probing gingival sulcus, reddened gingiva and no swelling); and 4 points (gum ulcerated or otherwise).

3. Plaque index (PLI)

Grading 4 according to the amount of bacterial plaque, 0 (no bacterial plaque in the gingival margin); 1 point (no plaque is detected by visual inspection, but a thin layer of plaque can be scraped in a free gum and the adjacent area by a probe); 2 points (moderate accumulation of soft deposits can be seen in the pocket, free gum area or adjacent tooth surface); 3 points (large soft deposits in the gingival pocket or free gingival area and adjacent tooth surface).

Fourth, test results

GI. The values of SBI and PLI are the average values of the conditions of the volunteer subjects in each group, and the test results are shown in tables 3-5.

TABLE 3 comparison of GI values of test and control groups

Test set	Before the experiment	After 4 weeks	After 8 weeks
				Test group	1.70±0.65	1.15±0.46	0.62±0.57
Control group I	1.58±0.38	1.52±0.45	1.44±0.54
				Control group II	1.66±0.47	1.52±0.49	1.13±0.60
Control group III	1.70±0.37	1.58±0.38	1.05±0.49
				Control group IV	1.59±0.88	1.45±0.59	1.11±0.37
Control group V	1.60±0.55	1.45±0.43	0.93±0.67

TABLE 4 comparison of SBI values of test and control groups

Test set	Before the experiment	After 4 weeks	After 8 weeks
				Test group	3.37±0.47	2.15±0.33	1.37±0.51
Control group I	3.45±0.73	3.33±0.57	3.12±0.45
				Control group II	3.52±0.45	3.02±0.44	2.81±0.20
Control group III	3.42±0.64	2.89±0.59	2.76±0.54
				Control group IV	3.39±0.98	2.94±0.45	2.83±0.61
Control group V	3.12±0.51	2.55±0.70	2.36±0.46

TABLE 5 comparison of PLI values in test and control groups

As can be seen from tables 3 to 5, compared with the toothpaste prepared by adding one or more of allantoin, tranexamic acid, cordyceps militaris essence, potassium nitrate and O-cymene-5-ol in comparative examples 1 to 5, the gum-protecting healing toothpaste disclosed by the invention can be used for effectively treating gingivitis, treating gingival bleeding more effectively, and relieving swelling and pain, and compared with a control group, the toothpaste has a significant difference (P is less than 0.05).

Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting the protection scope of the present invention, and although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.

Claims

1. A toothpaste for protecting gingiva and healing gingiva is characterized by comprising Cordyceps militaris essence, allantoin, tranexamic acid, O-cymene-5-ol, potassium nitrate and Zingiber zerumbet extract; the cordyceps militaris essence is a mixture of cordyceps militaris extract and cordyceps militaris sporocarp; the gingival protection and healing toothpaste comprises the following preparation raw materials in percentage by weight: 0.1-5% of cordyceps militaris essence, 0.1-2% of allantoin, 0.05-0.1% of tranexamic acid, 0.01-0.1% of O-cymene-5-alcohol, 3-10% of potassium nitrate and 0.1-1% of Zingiber zerumbet extract; the weight ratio of the cordyceps militaris extract to the cordyceps militaris sporocarp is as follows: cordyceps militaris extract: and (3) cordyceps militaris fruit body of 85-92: 8-15.

2. The gingiva-protecting and healing toothpaste according to claim 1, wherein the gingiva-protecting and healing toothpaste comprises the following raw materials prepared in percentage by weight: 0.5-3% of cordyceps militaris essence, 0.2-0.8% of allantoin, 0.05-0.08% of tranexamic acid, 0.05-0.08% of O-cymene-5-alcohol, 3-5% of potassium nitrate and 0.1-0.5% of Zingiber zerumbet extract.

3. The gingival protection healing toothpaste of claim 1, wherein the gingival protection healing toothpaste is prepared from materials further comprising a humectant, an abrasive, a thickener, and a foaming agent.

4. The gingival protection and healing toothpaste as claimed in claim 3, wherein the gingival protection and healing toothpaste is prepared from 40 to 60 weight percent of the humectant, 14 to 30 weight percent of the abrasive, 0.5 to 3 weight percent of the thickener and 1.8 to 3 weight percent of the foaming agent.

5. The gingival healing toothpaste of claim 3, wherein the foaming agent is a mixture of sodium lauryl sulfate and at least one of an amino acid surfactant and an amphoteric surfactant.

6. The gum healing toothpaste of claim 3, wherein the foaming agent is a mixture of sodium lauryl sulfate and at least one of sodium lauroyl sarcosinate, sodium lauroyl methyltaurate and cocamidopropyl betaine.

7. The gingival protection and healing toothpaste of claim 3, wherein the abrasive is at least one of precipitated silica, ultra-fine silica, calcium hydrogen phosphate, and calcium carbonate.

8. The gingival protection and healing toothpaste as claimed in claim 3, wherein the abrasive is a mixture of at least one of precipitated silica, calcium hydrogen phosphate and calcium carbonate and superfine silica, and the gingival protection and healing toothpaste is prepared from raw materials containing 2-6 wt% of the superfine silica.

9. The gum care healing toothpaste of claim 3, wherein the thickening agent is at least one of sodium carboxymethylcellulose, carbomer, and xanthan gum; the humectant is at least one of sorbitol, glycerol, polyethylene glycol and propylene glycol.