CN111743842A - Novel biological plant mouthwash - Google Patents

Abstract

The invention relates to a novel biological plant gargle, which is mainly prepared by taking extracts of plants such as rhizoma kaempferiae, lonicera confusa and selfheal as main sterilization components, adding biological enzyme components, and compounding to obtain the high-efficiency sterilization and odor removal gargle. Has the following characteristics: (1) the oral liquid has broad-spectrum antibacterial effect and effectively aims at various bacteria breeding in the oral cavity; (2) the biological plant formula has no damage to oral mucosa and dental minerals, has no chemical residue, and can be frequently used; (3) can prevent the occurrence of dental ulcer, dental plaque, gingivitis and periodontitis; (4) the antibacterial effect is long, the halitosis phenomenon is reduced, and the breath is refreshed; (5) convenient use, natural plant fragrance and convenient carrying when going out.

Description

Novel biological plant mouthwash

The invention relates to a novel biological plant gargle, which is mainly characterized in that refined substances extracted from plants such as rhizoma kaempferiae, lonicera confusa, selfheal, radix asparagi, semen plantaginis, bighead atractylodes rhizome, radix paeoniae alba and ramulus mori are used as main sterilization components, and biological component lysostaphin and biological enzyme are added for compounding to obtain the high-efficiency sterilization and odor-removal gargle. The mouthwash is one of the modes of cleaning oral health, generally can control the generation of bacterial plaque on the basis of effectively brushing teeth, and can penetrate into all parts of the oral cavity, reduce bacteria and bacterial plaque causing halitosis, and improve the gum health. In these days, mouthwash is becoming the most important auxiliary means for maintaining oral health represented by 80 and 90 days, and the penetration rate of mouthwash is higher among high scholars, and in addition, the demand of smokers for mouthwash is very large. Investigation shows that the incidence of halitosis in the current society is very high, 80-90% of halitosis and halitosis are originated from oral cavity, and untreated decayed tooth, residual root, residual crown, bad prosthesis, abnormal anatomical structure, gingivitis, periodontitis, oral mucosa diseases and the like in the oral cavity can cause halitosis. The large surface area of the back of the tongue has a plurality of papillae, fissures and pits, which are beneficial to the retention of bacteria, oral mucosa cast-off epithelium, food residues and the like, and serve as a 'bacteria storage chamber' which is beneficial to the generation of halitosis. However, these bad breath are not solved by rinsing mouth, and especially for people who often go out to communicate with people, it is very important to remove oral bacteria and reduce bad breath. The types of gargle in the market are very many, the gargle contains a prescription containing a medicinal component and an over-the-counter type containing a non-medicinal component, the gargle containing the medicinal component cannot be frequently used and is easy to damage oral mucosa and dental minerals, most of the non-prescription gargle contains mint essence as a main raw material, the effect of sterilization cannot be fundamentally achieved, and the gargle has no relieving and preventing effects on gingiva and periodontitis. Therefore, a novel biological plant gargle which can fundamentally remove oral bacteria and oral peculiar smell and can prevent gingivitis and periodontitis is needed.

The oral cavity is an absolutely bacteria-bearing environment, and food residues and weakly alkaline saliva provide a proper reproduction environment for bacteria in the oral cavity. If not cleaned in time, oral bacteria can be harmful to health. The bacteria with the largest proportion are streptococcus mutans, are one of main components of dental plaque and are also main pathogenic bacteria of caries, the caries is a bacterial infectious disease occurring in hard tissues of teeth, and the incidence rate is the highest in oral diseases; the second more common flora is staphylococcus, which is a group of gram-negative cocci, often piled up in the shape of a string, most of which are non-pathogenic bacteria, and the minority of which can cause diseases and cause oral inflammation. Tooth brushing is the most commonly used mode for cleaning the oral cavity in daily life, but people who have serious oral odor and incomplete tooth brushing cause troubles when going out to communicate with people. The history of mouth washing is longer than that of tooth brushing, although the use of the toothbrush and the dental floss is an effective measure for removing dental plaque, the method for controlling the dental plaque is difficult to master, tedious and time-consuming, difficult to finish especially for patients with physical and mental disabilities, difficult to adopt for people without motivation, and inconvenient to carry and use when going out. Mouthwash is an ideal alternative for this group of people. Practice proves that the mouthwash is convenient to use, easy to master, practical and feasible, and is suitable for daily oral care at home and for work and business trip. Particularly, people who seek high-quality life want to refresh their breath by various means, and the mouthwash can achieve this effect in a short time.

The types of mouthwashes common in the market are very various, the performance and the price are also different, most of the mouthwashes contain fluoride, and the mouthwashes contain 0.05% of sodium fluoride, so that extra fluoride can be provided for people in need. The tooth-protecting toothpaste can provide additional protection for teeth by being used once a day, and can effectively prevent decayed teeth, but only aims at people with demand, and the health of the normal people is influenced due to overhigh fluorine content; also, a common gargle is prepared from chlorhexidine gluconate as the main ingredient, which is proved to be effective in inhibiting the growth of dental pellicle and preventing periodontal disease, but if it is used for a long time, it will cause dental stains to be easily deposited on the tooth surface, affecting the sense of taste and causing recurrent aphthae; the herbal gargle with the mildest property adopts pure plant components, does not contain alcohol, antibiotics, Yujie pure and other broad-spectrum disinfection and sterilization chemical components, has small irritation to oral mucosa, is suitable for the old, the young and pregnant women, but has low sterilization rate and unobvious effect, and is not accepted by wide consumers. Therefore, people urgently need a novel biological plant mouthwash which can fundamentally inhibit the breeding of oral bacteria, remove oral peculiar smell and has no stimulation to oral mucosa. The invention aims to provide the mouthwash which can quickly remove bacteria, remove peculiar smell in the oral cavity, refresh breath and has mild property, and can prevent dental plaque, gingivitis and periodontitis.

The key point of the invention is that lysostaphin and bio-enzyme with bactericidal components are compounded with a plurality of plant components with bacteriostatic action and act together in the oral cavity to inhibit the propagation of pathogenic bacteria in the oral cavity, remove the peculiar smell of the oral cavity and prevent the occurrence of oral diseases. The formula selected by the invention is as follows (components and weight percentage): 3.0-5.0 u/g of lysostaphin, 4.0-6.0 u/g of biological enzyme, 3.0-6.0 parts of rhizoma kaempferiae, 6.0-15.0 parts of lonicera confusa, 5.0-7.0 parts of selfheal, 2.0-3.0 parts of radix paeoniae alba, 5.0-8.0 parts of radix asparagi, 7.0-9.0 parts of rhizoma atractylodis macrocephalae, 5.0-8.0 parts of semen plantaginis, 1.0-2.0 parts of ramulus mori, 1.0-3.0 parts of amomum tsao-ko, 3.0-4.0 parts of sodium chloride and the addition of sterile deionized water to 100 parts.

The lysostaphin is zinc-containing metalloprotease, consists of 246 amino acids, and the nucleic acid for expressing the gene consists of 1486 nucleotides, the isoelectric point is 10.5-11.0, the optimum pH range of the enzyme activity is 10-11, so that the lysostaphin can specifically degrade bacteria with cell walls having a glycine peptide bond structure, such as staphylococcus aureus, and the like, can achieve the purpose of sterilization by cracking the cell walls, and can effectively kill the staphylococcus aureus causing symptoms, such as gingivitis, periodontitis, and the like. The biological enzyme is active enzyme with broad-spectrum antibacterial action, can quickly permeate into cell membranes of bacteria, changes osmotic pressure of the bacteria, and leads the bacteria to lose activity and die.

The volatile oil and partial monomer compound of the kaempferia galanga have a series of physiological activities of resisting inflammation, resisting oxidation, resisting and preventing cancer, killing nematodes and the like. Experiments prove that kaempferide contained in the kaempferia galanga has an inhibiting effect on staphylococcus aureus, typhoid bacillus, pseudomonas aeruginosa, dysentery bacillus and the like, has an effect of inhibiting inflammation of a rat implanted in a wool ball, contains a plurality of phenolic hydroxyl groups, and has a strong antioxidation effect; and has effect in relieving toothache. The kaempferia galanga has a broad-spectrum antibacterial effect on common bacteria in the oral cavity, and the kaempferia galanga has natural fragrance, so that the peculiar smell of the oral cavity can be effectively improved, and the smell of the oral cavity is fresh.

Flos Lonicerae has effects of clearing away heat and toxic materials, and dispelling wind and heat; it is commonly used for carbuncle, swelling and sore, pharyngitis, erysipelas, heat-toxin and bloody dysentery, wind-heat type common cold, warm-heat type. The chlorogenic acid has high free radical scavenging effect, wide antibacterial effect, and can quickly permeate into bacterial membrane to crack bacteria and make them lose reproductive capacity.

The selfheal contains a large amount of triterpenoid saponin, rutin, hyperin and other glycoside substances and ursolic acid, caffeic acid, free oleanolic acid and other organic acids, and has an obvious anti-inflammatory effect; tests show that the decoction has certain inhibition effect on dysentery bacillus, typhoid bacillus, cholera vibrio, colibacillus, proteus bacillus, staphylococcus and human type tubercle bacillus in vitro; after the compound is compounded with the white paeony root, the effect is better through the synergistic effect. The radix asparagi decoction has an antibacterial effect on bacillus anthracis, alpha and beta hemolytic streptococcus, corynebacterium diphtheriae, diphtheria-like corynebacterium diphtheriae, diplococcus pneumoniae, staphylococcus aureus, staphylococcus limosum, staphylococcus albus and bacillus subtilis in vitro, so the radix asparagi decoction can effectively target bacteria in the oral cavity after being compounded with the selfheal and the white paeony root, and the antibacterial mechanism is as follows: the bacteria are wrapped to block the normal reproduction of the bacteria and cause the bacteria to die. And the asparagus contains a large amount of amino acids, so that the resistance of gum and oral mucosa can be improved, and the occurrence of oral diseases can be reduced.

The bighead atractylodes rhizome contains a large amount of volatile oil and amino acid components, has a high-efficiency bacteriostatic action, and the plantain seed is sweet and cold in nature and has the functions of clearing heat and removing toxicity.

The ramulus Mori contains a large amount of tannin components, and has effects of clearing heat and relieving swelling; the fructus Tsaoko contains a large amount of aromatic oil, effectively neutralizes the odor in oral cavity, and has halitosis removing effect.

After the plant components are compounded, respective antibacterial effects can be improved through synergistic effect, and biological enzyme components are added to increase active components of a system, so that the efficient and broad-spectrum antibacterial effect is achieved.

The preparation method of the product of the invention comprises the following steps:

(1) respectively cleaning, crushing and drying the plant components of rhizoma kaempferiae, lonicerae flos, selfheal, white paeony root, cochinchnese asparagus root, largehead atractylodes rhizome, plantain seed, mulberry twig and amomum tsao-ko for later use;

(2) respectively mixing the plant components with purified water in a ratio of 1:3, and heating and decocting the mixture with strong fire to a ratio of 1:1 to obtain plant essence extracting solution;

(3) respectively cooling, filtering and centrifuging the plant essence extract to obtain plant essence pure liquid;

(4) adding the sterile deionized water into a stirring tank according to the proportion;

(5) adding sodium chloride into a stirring tank according to a proportion, and heating and stirring uniformly;

(6) respectively adding plant essence pure solutions of rhizoma kaempferiae, flos lonicerae, selfheal, radix paeoniae alba, radix asparagi, rhizoma atractylodis macrocephalae, semen plantaginis, ramulus mori and fructus tsaoko in proportion into a stirring tank, and uniformly stirring;

(7) stopping heating, respectively adding the biological enzyme and the lysostaphin into a stirring tank according to the proportion, and uniformly stirring;

(8) stopping stirring, cooling and then packaging under aseptic condition.

The invention has the following advantages:

(1) the oral liquid has broad-spectrum antibacterial effect and effectively aims at various bacteria breeding in the oral cavity;

(2) the biological plant formula has no damage to oral mucosa and dental minerals, has no chemical residue, and can be frequently used;

(3) can prevent the occurrence of dental ulcer, dental plaque, gingivitis and periodontitis;

(4) the antibacterial effect is long, the halitosis phenomenon is reduced, and the breath is refreshed;

(5) convenient use, natural plant fragrance and convenient carrying when going out.

The specific implementation mode is as follows: the present invention will be described in further detail with reference to the following examples:

the formulation was prepared according to the following formulation data (in% by weight) and the procedures described:

the formula I is as follows: 3.0-5.0 u/g of lysostaphin, 4.0-6.0 u/g of biological enzyme, 3.0-6.0 parts of rhizoma kaempferiae, 6.0-15.0 parts of lonicera confusa, 5.0-7.0 parts of selfheal, 2.0-3.0 parts of radix paeoniae alba, 5.0-8.0 parts of radix asparagi, 7.0-9.0 parts of rhizoma atractylodis macrocephalae, 5.0-8.0 parts of semen plantaginis, 1.0-2.0 parts of ramulus mori, 1.0-3.0 parts of amomum tsao-ko, 3.0-4.0 parts of sodium chloride and the addition of sterile deionized water to 100 parts.

And a second formula: 3.0-4.0 u/g of lysostaphin, 4.0-5.0 u/g of biological enzyme, 3.0-4.5 of rhizoma kaempferiae, 6.0-10.5 of lonicera confusa, 5.0-6.0 of selfheal, 2.0-2.5 of radix paeoniae alba, 5.0-6.5 of radix asparagi, 7.0-8.0 of rhizoma atractylodis macrocephalae, 5.0-6.5 of semen plantaginis, 1.0-1.5 of ramulus mori, 1.0-2.0 of amomum tsao-ko, 3.0-3.5 of sodium chloride and adding sterile deionized water to 100.

And the formula III: 4.0-5.0 u/g of lysostaphin, 5.0-6.0 u/g of biological enzyme, 4.5-6.0 parts of rhizoma kaempferiae, 10.5-15.0 parts of lonicera confusa, 6.0-7.0 parts of selfheal, 2.5-3.0 parts of radix paeoniae alba, 6.5-8.0 parts of radix asparagi, 8.0-9.0 parts of rhizoma atractylodis macrocephalae, 6.5-8.0 parts of semen plantaginis, 1.5-2.0 parts of ramulus mori, 2.0-3.0 parts of amomum tsao-ko, 3.5-4.0 parts of sodium chloride and the addition of sterile deionized water to 100 parts.

Claims (2)

1. The invention relates to a novel biological plant mouthwash which is characterized by comprising the following components in percentage by weight: 3.0-5.0 u/g of lysostaphin, 4.0-6.0 u/g of biological enzyme, 3.0-6.0 parts of rhizoma kaempferiae, 6.0-15.0 parts of lonicera confusa, 5.0-7.0 parts of selfheal, 2.0-3.0 parts of radix paeoniae alba, 5.0-8.0 parts of radix asparagi, 7.0-9.0 parts of rhizoma atractylodis macrocephalae, 5.0-8.0 parts of semen plantaginis, 1.0-2.0 parts of ramulus mori, 1.0-3.0 parts of amomum tsao-ko, 3.0-4.0 parts of sodium chloride and the addition of sterile deionized water to 100 parts.

2. A novel bio-plant mouthwash according to claim 1, characterized by the following steps:

(1) respectively cleaning, crushing and drying the plant components of rhizoma kaempferiae, lonicerae flos, selfheal, white paeony root, cochinchnese asparagus root, largehead atractylodes rhizome, plantain seed, mulberry twig and amomum tsao-ko for later use;

(2) respectively mixing the plant components with purified water in a ratio of 1:3, and heating and decocting the mixture with strong fire to a ratio of 1:1 to obtain plant essence extracting solution;

(3) respectively cooling, filtering and centrifuging the plant essence extract to obtain plant essence pure liquid;

(4) adding the sterile deionized water into a stirring tank according to the proportion;

(5) adding sodium chloride into a stirring tank according to a proportion, and heating and stirring uniformly;

(6) respectively adding plant essence pure solutions of rhizoma kaempferiae, flos lonicerae, selfheal, radix paeoniae alba, radix asparagi, rhizoma atractylodis macrocephalae, semen plantaginis, ramulus mori and fructus tsaoko in proportion into a stirring tank, and uniformly stirring;

(7) stopping heating, respectively adding the biological enzyme and the lysostaphin into a stirring tank according to the proportion, and uniformly stirring;

(8) stopping stirring, cooling and then packaging under aseptic condition.