KR20100027746A - Oral composition containing natural agent - Google Patents

Abstract

PURPOSE: A dental composition containing pine needle extract and myrrh extract is provided to ensure antibacterial synergic effect and remove oral cavity odor. CONSTITUTION: A dental composition contains 0.001-2.0 weight% of pine needle extract and 0.001-2.0 weight% of myrrh extract. The composition further contains 0.001-2.0 weight% of salt. A method for preparing pine needle extract comprises: a step of cutting the pine needle in a length of 1.0-2.0 cm; a step of immersing in 70% of ethanol solution at room temperature for 7 days; and a step of decompress-concentrating.

Description

Oral composition containing natural extracts

The present invention relates to a composition for oral cavity containing pine needle extract and myrrh extract, and a composition for oral cavity further containing salt in the composition, more specifically pine needle extract with excellent antibacterial ability to effectively control the bacteria in the oral cavity and It relates to a composition for oral cavity containing myrrh extract and salt which is effective in gum disease.

In general, bad breath occurring in the oral cavity is usually produced by hydrolysis of proteins and peptides containing sulfur components by Gram-negative bacteria in a basic environment. Sulfur compounds generated through this process are hydrogen sulfide, methylmercaptan, dimethyl sulfide, and the like. The conversion of gram-positive bacteria to gram-negative and anaerobic bacteria is associated with the generation of odors. Spherical factors, such as decreased saliva secretion or elevated oral pH, can affect this conversion process. Failure to effectively and consistently remove the bacterial membranes present in the interdental gram-negative bacteria that remain there can break down sulfur-containing amino acids, resulting in odors. Periodontitis can also contribute to bad breath because substrates produced during the inflammatory process promote bacterial growth. In addition, the decay process and accompanying odors occur more rapidly when a lot of bacteria accumulate on the tongue. The progression of periodontitis and deep periodontal sac also contribute to bad breath. Therefore, management of periodontitis is an important part of the management of posterior teeth.

Therefore, in order to maintain the bad breath for a long time, it is necessary to sterilize the bacteria in the oral cavity causing the bad breath to prevent bad breath.

In addition to bad breath, common oral diseases such as caries and periodontal disease is known to be caused by the plaque produced by the saliva buried in the tooth surface to form a sticky thin film, the oral microorganisms attached to it form a colony. That is, caries is caused by the enamel corrosion caused by the dissolution of hydroxyapatite, a major component of teeth, by organic acids produced by anaerobic bacteria in the plaque and metabolism of saccharides. This is accompanied by accumulation and consequent increase in bacterial activity and weakening of host defenses. Therefore, to alleviate periodontal disease, the activity of periodontal bacteria must be suppressed to reduce inflammation in the gums.

Although oral products containing various fungicides such as cetylpyridinium chloride and chlorohexidine hydrochloride are known to sterilize harmful bacteria in the oral cavity, chlorohexidine hydrochloride has the disadvantage of causing tooth discoloration and cetylpyridinium silver chloride. Is a compound with a cation, and due to its strong reactivity with anionic materials, the effect may be halved depending on the oral environment in actual use.

In addition, the effects of salt on the oral cavity are generally known to be effective in preventing or treating oral diseases, particularly gingivitis and alveolar pylori, as a convergent effect on the gums when salt is used in the oral cavity. A number of oral compositions containing salt have been proposed for the purpose of preventing and treating such gum diseases.

Therefore, an object of the present invention is to effectively sterilize the bacteria in the oral cavity to exhibit an excellent bad breath removing effect, and to provide a composition for oral cavity which is effective in gum disease.

In order to achieve the above object, the present invention provides a composition for oral cavity containing pine needle extract and myrrh extract.

The present invention also provides a composition for oral cavity, characterized in that it further contains salt in the composition.

The composition for oral cavity prepared by the present invention provides an excellent bad breath removing effect over a long and short period of time, and can provide an excellent gum disease prevention effect, caries prevention effect and the like in the oral cavity.

The composition for oral cavity of this invention contains pine needle extract and myrrh extract as a bactericidal and antimicrobial component.

In addition, the composition for oral cavity of the present invention further contains salt as a gum disease prevention component in the composition.

Hereinafter, the preparation of the composition for oral cavity of the present invention will be described.

Pine needle extract used in the present invention is as follows.

Pine is a genus of about 80-90 species in the world, and the terpenes collected from them are mainly used in industry. The main components of pine needles are Terpennetine oil, Cineole, Salinigrin, Coniferin, P-Cymen, Densipimaric acid, Retene, chlorophyll, protein, crude fat, phosphorus, iron, enzymes, minerals, fat-soluble vitamin A and vitamin C, etc., and the main components of pine needles vary slightly depending on the species and season taken. . Terpentin oil is a group of unsaturated hydrocarbons having a chemical formula of C ₁₀ H ₁₆ as a volatile fragrance and is known for its bactericidal and antimicrobial activity.

The main components of pine leaf essential oil extracted by steam distillation from pine leaves and soft branches are monoterpenes and sesquiterpenes. Among the monoterpenes, alpha-pinene and beta-pinene ), Karen (Carene), beta-myrcene (β-Myrcene), limonene (Limonene) and other ingredients are included.

Pine trees have also been closely linked to the real life of our nation since ancient times. In particular, pine needles are most widely used because they are easier to collect and simple to prepare than other parts. In fact, pine needles are rich in general nutrients, amino acids, fatty acids, vitamins and minerals, so pine needle powder is sold as a healthy food. Currently, pine needles are used in various ways such as green juice, alcohol, sweet medicine, decoction, tea, sweets and health drinks.

These pine needles have been used as a folk remedy known empirically for various effects. Among those related to oral disease, bad breath was chewed by washing pine needles in water, and in stomatitis, pine needles were put on cotton wool and applied to affected areas. In addition, the toothache is brushed with boiled water, the gums are swollen, and the salt is added to the boiled salt, which is then bitten in the mouth, and the thrush is given a handful of pine needles, and the juice is brushed in the mouth and brushed. Brushing your teeth with boiled water is said to be effective.

Pine needle extract used in the present invention may be prepared by a conventional method known in the art to which the present invention belongs, preferably 50% of the pine needles collected to cut 1.0-2.0cm size 70% ethanol solution ( A mixture of water and ethanol) was added at a rate of 500 ml, and the mixture was immersed at room temperature for 7 days, extracted, filtered, and the filtrate was concentrated under reduced pressure to obtain a concentrate.

Myrrh extract used in the present invention is as follows.

Myrrh is also called myrrha, and it is also called soft myrrh to distinguish it from flower pots used in dyes. Pale yellow or dark brown large and small lumpy substance, dissolved in alcohol and used as a miral tincture for mouthwash. Contains essential oils, resins, and rubber. It has long been used as a preservative, and it is a well-known fact that it was used a lot when making mummies. It is also well known that in the New Testament Gospel of Matthew (2:11), the Magi gave Baby Jesus with frankincense and gold.

Such a myrrh is Commphora molmol Engl. And rubber resins obtained from wounds of the bark of the same plant (Olivaceae), which have analgesic and antiseptic properties, and have been used for thrush, gingivitis and mouthwashes.

Myrrh extract used in the present invention may be prepared by a conventional method known in the art to which the present invention belongs, preferably 1 kg of natural Oleo-gum-resin of Commphora species (species) It was crushed into small pieces, and the water vapor was flowed for 2 hours at a rate of 0.5 L / min under a pressure of 1.5 kg / cm ² , and then the water vapor was cooled by passing through a cooler, and then the extract collected in the water flask was separated from the water layer and 90 g of the extract was extracted. Get Here, edible ethane is obtained by admixing so that the extract concentration is 50%.

The salt used in the present invention is not limited in kind, but preferably salt, refined salt, baked salt, or the like is used.

The composition for oral cavity of the present invention contains the pine needle extract obtained by the above method in an amount of 0.001 to 2.0% by weight relative to the total weight of the composition, the myrrh extract in an amount of 0.001 to 2.0% by weight relative to the total weight of the composition, wherein It may further contain in an amount of 0.001 to 10.0% by weight based on the total weight of the composition.

The composition for oral cavity of the present invention has an excellent antibacterial and bad breath reducing effect by inhibiting bacteria in the oral cavity by synergistic effects of pine needle extract and myrrh extract, and provides a periodontal disease prevention effect by the convergence effect on the gums of salt.

The composition for oral cavity of this invention is not specifically limited in the formulation, Specifically, it may have formulations, such as a toothpaste, mouthwash, or mouthwash.

The compounding component of the composition for oral cavity used in the present invention is changed depending on the use and type of the composition in addition to the pine needle extract mole, myrrh extract and salt described above.

Various oral compositions for carrying out the present invention may use a suitable component according to the type, for example, if the formulation is a toothpaste, as a conventional toothpaste components, abrasives, wetting agents, foaming agents, binders, sweeteners, pH adjusting agents, preservatives, It may contain an active ingredient, a fragrance, a brightener, a dye, a solvent and the like.

Examples of the abrasive include calcium carbonate, precipitated silica, aluminum hydroxide, calcium monohydrogen phosphate, and insoluble sodium metaphosphate. These abrasives may be used alone or in combination of two or more kinds thereof, preferably 1 to 60% by weight, preferably 10 to the total weight of the composition. 50 weight% is used.

As the humectant, glycerin, sorbitol solution, polyethylene glycol, propylene glycol and the like are mixed alone or in combination of two or more, and 10 to 60% by weight, preferably 20 to 50% by weight, based on the total weight of the composition is used. As the foaming agent, anionic and nonionic surfactants such as sodium lauryl sulfate, sodium lauryl sarcosinate, sucrose fatty acid ester, polyoxyethylene hardened castor oil, and polyoxyethylene polyoxypropylene copolymer may be used alone or in combination of two or more thereof. 0.5 to 5% by weight, preferably 0.5 to 3% by weight, based on the total weight.

As the binder, carboxymethyl cellulose sodium, hydroxymethyl cellulose, carrageenan, xanthan gum, sodium alginate, etc. may be used alone or in combination of two or more thereof, and 0.1 to 5% by weight, preferably 0.1 to 2% by weight, based on the total weight of the composition is used. . As a sweetening agent, saccharin sodium, aspartame, stevioside, ziitol, and licorice may be used alone or in combination of two or more, and 0.05 to 5% by weight based on the total weight of the composition is used.

The pH adjusting agent includes sodium phosphate, disodium phosphate, trisodium phosphate, sodium pyrophosphate, citric acid, sodium citrate, tartaric acid, and the like, and preservatives such as methyl paraoxybenzoate, propyl paraoxybenzoate and sodium benzoate alone or two or more. Use by mixing.

The active ingredients include sodium fluoride, sodium monofluorophosphate, tin fluoride, chlorohexidine, allantoin chlorohydroxy aluminate, aminocaproic acid, tranexamic acid, triclosan, cetylpyridium chloride, zinc chloride, pyridoxine hydrochloride, acetic acid Tocopherol or the like may be used alone or in combination of two or more thereof.As a fragrance, peppermint oil, spearmint oil, menthol, and anetol may be mixed in an appropriate amount.Titanium oxide is used as a brightener, food coloring is used as a pigment, purified water and A solvent of ethanol is prepared according to a conventional toothpaste manufacturing method.

Hereinafter, the composition and the effect of the composition for oral cavity according to the present invention, for example and comparative examples will be described in more detail. However, the present invention is not limited only to these examples.

[Examples and Comparative Examples]

Toothpaste of Examples 1 to 2 and Comparative Examples 1 to 3 were prepared according to a conventional manufacturing method in the composition of Table 1 below.

ingredient Example 1 Example 2 Comparative Example 1 Comparative Example 2 Comparative Example 3 Sodium fluoride 0.30 0.30 0.30 0.30 0.30 Precipitated silica 17.00 17.00 17.00 17.00 17.00 Pine needle extract 0.10 0.10 0.10 - Myrrh Extract 0.10 0.10 - - 0.10 Refined salt 1.00 Concentrated glycerin 3.00 3.00 3.00 3.00 3.00 Sorbitol solution 10.00 10.00 10.00 10.00 10.00 Carboxymethyl Cellulose Sodium 1.00 1.00 1.00 1.00 1.00 Zaccarin Sodium 0.10 0.10 0.10 0.10 0.10 Paraoxybenzoic Acid Methyl 0.20 0.20 0.20 0.20 0.20 Titanium oxide 0.10 0.10 0.10 0.10 0.10 Spices 1.00 1.00 1.00 1.00 1.00 Sodium Lauryl Sulfate 2.00 2.00 2.00 2.00 2.00 Purified water To 100 To 100 To 100 To 100 To 100

Test Example 1 Antibacterial Activity Test

Example 1 and Comparative Examples 1 to 3 were diluted 2 ml of the test solution from 1:20 to 1: 5120 and 18 ml of blood agar medium (blood agar base + final blood 5% sheep) in a 9 cm petri dish. Poured. Thereafter, test bacteria were inoculated and incubated at 37 ° C. for 96 hours using an anaerobic chamber. The maximum dilution rate at which the test bacteria did not grow was used as the MID (using the Bactron I. anaerobic chamber), and the maximum bacterial dilution rate (MID) was shown in Table 2 below.

<Test bacteria>

① Bad breath cause: Fusobacterium nucleatum ATCC 25586, Porphyromonas gingivalis ATCC 33277

② Cause of caries: Streptococcus mutans NCTC 10449, Actinomyces viscosus ATCC 15987

③ Main disease causing bacteria: Actionbacillus actinomycetemcomitans ATCC 29522

Maximum bacterial dilution rate (MID) Test bacteria Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Fusobacterium nucleatum 2560 > 20 160 320 Porphyromonas gingivalis 2560 > 20 320 160 Streptococcus mutans 1280 > 20 320 640 Actinomyces viscosus 5120 20 640 1280 Actionbacillus Actinomycetemcomitans 1280 > 20 160 160

As can be seen from the results of Table 2, the composition of Comparative Example 1, which does not contain any active ingredient, showed almost no inhibitory effect on oral harmful bacteria, indicating that there was little antibacterial effect, and Comparative Examples containing pine needle extract Comparative Example 3 containing 2 and myrrh showed a more effective antimicrobial effect than Comparative Example 1. However, Example 1, in which pine needle extract and myrrh extract are used in combination, shows excellent antimicrobial effect against various oral harmful bacteria compared to Comparative Examples 2 and 3. Through this, the combined use of pine needle extract and myrrh extract showed a synergistic effect in increasing antimicrobial activity.

Test Example 2 Measurement of the Number of Bacteria in Saliva

5 ml of sterilized 0.1% peptone solution (1 g of peptone was added to 1000 ml of 0.9% NaCl) was added to a 15 ml Falcon tube, and 40 were prepared. After 15 seconds of hydration, the peptone solution was diluted 10-fold with sterile 0.067 M Phosphate buffer solution (pH 7.2). Take 100 μl of this diluted solution, dispense into Mitis Salivarius medium (Agar), spread the plate entirely on a plate with sterile bending glass, incubate for 12 hours in a 37 ° C. incubator, and then anaerobic chamber. Incubate again at 37 ° C. for 48 hours, and then in each medium using a Colony counter. Streptococcus Colonies of mutans were measured and recorded. Streptococcus Colonies of mutans were identified by microscopic examination, and the standard strain Streptococcus mutans NTCC 10449 to check the form of the cultured colony in the time and under the same conditions as in the MSA, it was measured by selecting only the colonies of Streptococcus mutans in the test subjects. Diluted peptone poloxes were dispensed into two plates of Mitis Salivarius medium per test subject, and colony measurements were performed twice and twice, and the mean value of the two measurements was taken as the number of Streptococcus mutans of the subjects. The measured results are shown in Table 3 below.

Determination of the number of bacteria in saliva Elapsed time Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Right after the experiment 109 108 110 112 after 2 weeks 21 98 65 54

As can be seen from the results of Table 3, the composition of Comparative Example 1 containing no pine needle extract and myrrh extract is Streptococcus The number of mutans hardly decreased, and in the case of Comparative Example 2 containing pine needle extract or Comparative Example 3 containing myrrh extract, the numerical value was lower than that of Comparative Example 1. However, in the case of Example 1 in which pine needle extract and myrrh extract are used in combination, it can be seen that the results are excellent compared to Comparative Examples 2 and 3. From this, it was confirmed that the combined use of pine needle extract and myrrh extract was effective to greatly reduce the number of bacteria due to the synergistic effect.

Test Example 3 Measurement of Bad Breath Removal Effect

After 80 hours, all oral environment management was stopped for 48 hours, and the degree of bad breath was measured using a harimetta, and divided into 4 groups so that the average value was similar. For each group, Examples 1 and 2 and Comparative Examples 1 to 3 were allowed to brush with test fern. At 30 minutes after brushing, the degree of bad breath was measured again. Each test fern was then used twice a day for 28 days. After 7 days, 14 days, 21 and 28 days elapsed, the degree of bad breath was measured again 30 minutes after brushing, and the effect of reducing the bad breath according to the use period of the toothpaste composition is shown in Table 4 below.

Bad Breath Scores by Progression Elapsed time Example 1 Example 2 Comparative Example 1 Comparative Example 2 Comparative Example 3 Right after the experiment 220 220 219 218 221 7 days 170 150 222 205 208 14 days 145 130 217 196 195 21st 115 103 219 185 187 28 days 102 96 217 174 176

Looking at the results of Table 4, the bad breath score of the oral composition of Comparative Example 1 in which no medicinal ingredients were used was almost unchanged. On the other hand, the bad breath score of the composition of Example 1 in combination with pine needle extract and myrrh extract was significantly reduced. In the case of Comparative Example 2 and Comparative Example 3 using pine needle extract and myrrh extract, respectively, the reduction effect was observed, but the effect was not so great when compared with Example 1 used in combination. Furthermore, Example 2 using salt together with pine needle extract and myrrh extract was able to confirm that the effect is significantly different.

[Test Example 4] Measurement of gum disease prevention effect

의 치은염 평점 기준에 따라 측정하였다.To determine the effect of preventing gum disease, the gingival index was measured and the method was as follows. First, 60 subjects were screened, and during the experiment period, the administration of antibiotics and the receipt of oral medical treatments that may affect the experiment were prohibited. Twenty subjects were divided into Comparative Example 1 and Examples 1 and 2, and the gingivitis index was modified by Talbott, Mandel and Chilton.

It was measured according to the gingivitis grading criteria.

의 치은염 지수><Transformed by Talbott, Mandel and Chilton

Gingivitis Index>

의 치은염평점기준

Gingivitis rating criteria

0: no inflammation

1: Mild gingivitis (weak change of color and weak edema)-no bleeding during periodontal cyst measurement

2: moderate gingivitis (redness, edema)-bleeding during periodontal cystic measurement

3: highly gingivitis (ulcer)-tendency to bleed on its own

Gingival index Comparative Example 1 Example 1 Example 2 Initial value 1.60 ± 0.21 1.58 ± 0.21 1.61 ± 0.21 2 weeks 1.62 ± 0.25 1.32 ± 0.22 1.04 ± 0.14 4 Weeks 1.59 ± 0.22 1.13 ± 0.18 0.72 ± 0.16 Improvement Index 0.01 ± 0.23 0.45 ± 0.20 0.89 ± 0.18

Looking at the results of Table 5, Comparative Example 1 without the pine needle extract and myrrh extract and salt can be seen that there is little gingivitis mitigation effect. In the case of Example 1 in combination with pine needle extract and myrrh extract showed some gingival improvement effect, in the case of Example 2 in which salt was added to toothpaste, the gingival improvement effect was much more excellent. This is because the astringent action of salt on the gums increases the effect of preventing gum disease even more.

Claims (7)

Oral composition containing pine needle extract and myrrh extract. The oral composition according to claim 1, wherein the composition further contains salt. The composition for oral cavity according to claim 1, wherein the pine needle extract is contained in an amount of 0.001 to 2.0% by weight and the myrrh extract in an amount of 0.001 to 2.0% by weight based on the total weight of the composition. The composition for oral cavity according to claim 2, wherein the salt is contained in an amount of 0.001 to 10.0% by weight based on the total weight of the composition. The composition for oral cavity according to claim 1, wherein the composition has an antibacterial action. The composition for oral cavity according to claim 1 or 2, wherein the composition removes bad breath. The composition for oral cavity according to claim 1 or 2, wherein the composition improves gingival.