KR101635861B1 - Oral composition containing fermentative extract of galla rhoids as active ingredient - Google Patents

Abstract

The present invention relates to an oral composition containing a fermented extract of Galla rhoids as an active ingredient and is characterized in that it contains 0.0001 to 30.0% by weight of a fermented extract of Opuntia as an active ingredient based on the total weight of the oral composition . According to the present invention, the inclusion of the fermented extract of the obovate has the effect of inhibiting the growth of oral microorganisms, the effect of inhibiting the generation of acid, the prevention of dental caries, the elimination of bad breath, the relief of oral diseases, and the whitening effect.

Description

FIELD OF THE INVENTION [0001] The present invention relates to an oral composition containing a fermented extract of Opuntia ficus-indica,

The present invention relates to an oral composition capable of removing halitosis and alleviating oral diseases by having an antibacterial activity that can control bacteria in the oral cavity. The present invention also relates to an oral composition capable of preventing dental caries by inhibiting acid production and controlling cavitation-inducing bacteria. The present invention also relates to an oral composition having a tooth whitening effect that effectively removes contamination caused by external coloring of teeth.

Healthy teeth are one of the most important factors that determine the quality of life. Dental diseases include dental caries (dental caries), alveolar abscess, calculus, dental calculus, maxillary sinusitis, dental calculus, etc. It causes extreme physical pain, bad breath and yellow colored teeth are not good for cosmetics, It can cause a disruption.

It is known that about 600 to 800 or more microorganisms exist in human oral cavity, and these microorganisms are controlled by enzymes such as lysozyme secreted by saliva. However, nutrients and water-rich oral environments are good conditions for microbial growth, and dental plaques provide excellent microbial habits. Some of these microorganisms are opportunistic pathogens and cause dental cavities, periodontal disease, and bad breath.

 Enamel is a milky translucent, hard material that covers the surface of the head of a tooth and protects the tooth dentin. In dental caries, microorganisms forming plaque (dental plaque) are fermented with sugar and starch to make various organic acids such as lactic acid, which is caused by corrosion of enamel generated by dissolving hydroxyapatite, which is the main component of teeth Rise. The plaque is formed by the formation of a thin and sticky film on the teeth of the saliva, and the oral microorganisms such as Streptococcus sobrinus and Streptococcus mutans, which are a kind of streptococci, biofilm) and is thickened by Fusobacterium.

Periodontal disease is often referred to as taste, depending on the degree of disease gingivitis (gingivitis) and periodontitis (periodontitis) is divided into. It is a relatively light and fast-acting periodontal disease called gingivitis that is limited to gingiva or soft tissue, and it is called periodontitis when the inflammation progresses to the gums and the gums. The main cause of periodontal disease is due to the accumulation of excess plaque and the resulting increase in bacterial activity. The toxins and metabolites secreted by gram negative anaerobic bacteria destroy tissues or stimulate the immune system and cause inflammation. Also, when plaque and tartar build up, the gums fall from the teeth, and as a result, gaps are formed and a periodontal pouch is formed between the teeth and the gums. As the inflammation progresses, the gap between the gums and the teeth widens, the alveolar bone and the periodontal ligament are destroyed, and eventually the shaking teeth must be removed.

Generally, in the case of dental caries, chlorhexidine hydrochloride, cetylpyridinium chloride and the like are used to manage the plaque, but chlorhexidine hydrochloride causes tooth discoloration and cations, cetylpyridinium chloride, have a disadvantage in that they are ineffective when they react with an anionic substance.

A large amount of bacteria in the inside of the tongue causes a malodorous egg odor in the process of decaying food residues, dead cells, runny nose, etc. in the mouth and causing bad breath. Oral microorganisms such as Fusobacterium nucleatum and Porphyromonasgingivalis of halitosis have been found to contain unpleasant odors in the mouth due to volatile sulfur compounds resulting from degradation of proteins and peptides containing sulfur components Sulfur compounds such as hydrogen sulfide, methyl mercaptan, and dimethyl sulfide. In addition, oral dryness, or oral pH rise, white teeth, gingival diseases and also cause bad breath.

Such dental caries, periodontal diseases, bad breath, etc. are related to the proliferation of oral microorganisms. In order to prevent or heal them, it is necessary to sterilize the bacteria in the oral cavity to prevent them from occurring. In general, dental caries and periodontal disease use chlorhexidine hydrochloride, cetylpyridinium chloride and the like for the management of teeth, but chlorhexidine hydrochloride causes tooth discoloration and cations, cetylpyridinium chloride, have.

The causes of tooth discoloration include aging, overuse of antibiotics such as tetracycline, excessive intake of fluoride, jaundice, cerebral palsy, kidney damage, allergies, or endocrine diseases such as vitamin C, D, calcium, Tooth discoloration and exotic tooth discolorations caused by eating foods with high pigment such as coffee, tea, cocoa, kimchi, and jacobs, or by tobacco nicotine and tar.

To treat teeth whitening, carbamide peroxide, hydrogen peroxide, potassium nitrate, and fluoride are used. In addition, abrasives can be added to treat the teeth physically, or chemically treated with surfactants. In recent years, a special light beam is used to perform tooth whitening. The method developed in 1999 in the United States can make teeth white in a short time without side effects.

However, whitening treatment with peroxide is effective for discoloration caused by food, which is a genetic cause, but it has a disadvantage that it is less effective when discolored by taking antibiotics such as tetracycline. The method of using abrasive is a method of using excessive amount of abrasion There is a problem that it is possible to cause a wearer's abrasion.

Therefore, it is necessary to develop a safe natural material having antimicrobial activity against oral microorganisms causing oral diseases in order to solve tooth diseases as well as excellent tooth whitening effect.

DISCLOSURE OF THE INVENTION In order to solve the above problems, it is an object of the present invention to provide an oral composition having an excellent antimicrobial effect and an acid production inhibitory effect on oral microorganisms. It is another object of the present invention to provide an oral composition having a tooth whitening function by inhibiting the growth of oral microorganisms, preventing bad breath and oral diseases, inhibiting acid production of teeth and preventing dental caries.

The above-mentioned problem is solved by the oral composition comprising the fermented extract of Opuntia ficus as an active ingredient.

Preferably, the nut gall extract fermentation with Saccharomyces three Levy MY process jiae (Saccharomyces cerevisiae ATCC 18824) as an active ingredient.

Preferably, the oral composition may have a bad breath removing effect by controlling oral microorganisms.

Preferably, the composition for oral use can prevent dental caries by inhibiting acid production and controlling a tooth decay such as Streptococcus mutans.

Preferably, the oral composition can prevent oral diseases by inhibiting microorganisms causing periodontal disease.

Preferably, the oral composition may be for whitening teeth.

Preferably, the crude fermented extract may contain 0.0001 to 30.0% by weight, based on the freeze-dried weight, of the whole composition.

Preferably, the composition for oral cavity containing the fermentation extract of Opuntia ficus is not particularly limited in its formulation, but it may be a toothpaste, an oral cleanser, an oral cleanser, a gum, a candy, an oral spray, an oral ointment, ≪ / RTI >

The fermented extract according to the present invention has antimicrobial effect against oral microorganisms, inhibition of acid formation and prevention of dental caries, bad breath suppressing effect, oral disease mitigation effect and tooth whitening effect.

Unless defined otherwise, all technical terms used in the present invention have the following definitions and are consistent with the meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. Also, preferred methods or samples are described in this specification, but similar or equivalent ones are also included in the scope of the present invention.

The term " drug " is used in reference to a reference amount, level, value, number, frequency, percentage, dimension, size, amount, weight, or length of 30, 25, 20, 25, 10, 9, 8, 7, 6, Level, value, number, frequency, percentage, dimension, size, quantity, weight or length of a sample,

Throughout this specification, the words " comprising " and " comprising ", unless the context clearly requires otherwise, include the steps or components, or groups of steps or elements, And that they are not excluded.

The present invention is based on the finding that Galla Rhoids, a Rhizo javanica L. or Rhus javanica L. larvae, and a insect house caused by stings of aphan aphid (Melaphis chinensis Bell) , And fermented extracts were prepared from them. Antimicrobial effect and acid production inhibitory effect on oral microorganisms were measured. As a result, it was found that the efficacy of the fermented extract of Opuntia ficus was expected to be effective in preventing bad breath and dental caries, because the antimicrobial effect and the acid production inhibitory effect on oral microorganisms are excellent. In addition, it has been found that the gingivitis improvement effect on the human body is very excellent, so that the effect of preventing oral diseases can be expected. In addition, it has been found that tooth whitening effect can be expected because the tooth whitening effect is excellent.

The Galla Rhoids, Gallnut, which is used as an active ingredient in the oral composition of the present invention, is an insect habitat produced by parasitic on the leaves of Rhus (Rhododendron) There are many things that resemble human ears in the form of pockets. The shape is uneven, irregularly shaped like a bag with 2 to 4 cracks, or broken. The outer side is grayish grayish brown, covered with light grayish brown short hairs, hard and brittle. The inside is empty, and the taste is very good. The dwarf treats chronic seawater, old cough, old diarrhea, dehydration, self-inflicted sweat, cold sweat, and oil well, which are caused by the strong convergence of the lungs. . Convergence effect, antimicrobial action, anti-growth effect, hepatic function-protecting action and antioxidative action were reported by pharmacological action. It contains 50-60% of tannin component and becomes raw material of tannin, hair dye and ink including tannin.

In the present invention, the spermatogonial fermentation extract means obtained by fermenting the spermatogonial extract.

The dumbbells of the present invention are cleanly washed and finely pulverized to 100 to 500 mesh. In addition, the minerals may be dried before being crushed or used as they are, preferably dried.

In addition, a fermented extract obtained by fermenting microbes by inoculating them with a culture medium prepared by adding the above fermented extract to a conventional microbial culture medium is used.

The fermented extract according to the present invention may be prepared by adding 1 to 30% by weight of Rhus japonica extract to a culture medium, inoculating the fermented microorganism in an amount of 100,000 to 10,000,000 cfu / L, Followed by culturing at 35 占 폚 for 12 to 168 hours, preferably 12 to 72 hours.

The amount of the rhizome extract in the culture medium used for fermentation may be 1 to 30% by weight. If the amount of the extract is less than 1% by weight, the amount of the fermentation broth due to the microorganism is small and the effect is difficult to be exhibited. If the amount is more than 30% by weight,

The fermenting microorganism used in the present invention may be one or more selected from the group consisting of yeast, mushroom mycelium, lactic acid bacteria, and Bacillus sp., Preferably Saccharomyces sp. more preferably saccharose in my process three Levy jiae (Saccharomyces cerevisiae ATCC 18824).

After the fermentation, the culture is centrifuged to separate only the supernatant to obtain a fermented extract. The fermented extract thus obtained is used as it is, or the fermentation broth is dried and used. When it is dried, the extraction solvent is added 1 to 20 times as much as the dry weight, and the mixture is immersed at 4 to 30 ° C for 3 to 20 days to extract the active ingredient. Extraction with an extraction solvent may be carried out using any extraction solvent conventionally used in the art.

(A) water, an anhydrous or hydric alcohol having 1 to 4 carbon atoms (e.g., methanol, ethanol, propanol, and the like) And butanol), solvent extraction using a solvent selected from the group consisting of propylene glycol, 1,3-butylene glycol, glycerin, acetone, diethyl ether, ethyl acetate, butyl acetate, dichloromethane, chloroform, hexane, , (b) supercritical extraction by decompression by carbon dioxide and high temperature, or (c) extraction by ultrasonic extraction.

The extract was filtered through Whatman # 10 filter paper, and the filtrate was finally filtered using 0.25 μm filter paper. The filtrate was concentrated under reduced pressure and freeze-dried at 60 ° C or lower to obtain a filtrate, The fermentation extract is finally obtained using an extraction solvent such that it contains 70% by weight.

According to the present invention, the fermented extract of Rhododendron tumefaciens is contained in an amount of 0.0001 to 30.0% by weight based on the freeze-dried weight based on the total weight of the composition for oral use, more preferably, 0.01 to 10% by weight. When the content of the fermentation extract is less than 0.0001% by weight, the effect of improving oral hygiene is not exhibited. When the content of the fermented extract is more than 30.0% by weight, the degree of increase of oral hygiene improvement effect is insignificant, There is a problem and it is not economical.

In the present invention, the microorganism having the antimicrobial effect of the fermentation extract of Avalusia include Streptococcus mutans mutans , Fusobacterium < RTI ID = 0.0 > nulcleatum , Porphyromonas gingivalis , Actinomyces < RTI ID = 0.0 > viscosus , Actinobacillus actinobacillus ( Actinobacillus < RTI ID = 0.0 > actinomycetemcomitans , Streptococcus sanguinis , and the like.

The ingredients contained in the oral composition of the present invention may contain, as an active ingredient, the ingredients commonly used in oral compositions, in addition to the above-mentioned effective ingredients. Examples of such ingredients include abrasives, wetting agents, binders, foaming agents, sweeteners, preservatives, A coloring agent, a solvent, a brightener, a solubilizing agent or a pH adjusting agent.

The oral composition of the present invention may be prepared into any formulation conventionally produced in the art and may have a formulation such as, for example, toothpaste, mouthwash or mouthwash, gum, candy, mouth spray, But is not limited thereto.

As an example, when the oral composition of the present invention is a toothpaste formulation, it may contain a wetting agent, an abrasive, a binder, a foaming agent, a flavoring agent, a sweetener, a coloring agent, a preservative, a pharmaceutical composition, a solvent, a pH adjusting agent and the like.

The wetting agent is used to prevent the toothpaste from hardening in the air and to make the powder of the toothpaste composition paste-like and prevent the toothpaste from hardening in the air. The wetting agent may be used alone or in combination of two or more kinds of glycerin, sorbitol, propylene glycol, polyethylene glycol, %, Preferably 10 to 50% by weight.

The foaming agent is a surfactant which diffuses the toothpaste into the oral cavity to enhance the cleaning effect and acts as a surfactant to clean the oral contamination. It is also possible to use a surfactant such as sodium lauryl sulfate, sodium lauryl sarcosinate, sodium alkylsulfosuccinate, Or 0.5 to 10% by weight, preferably 0.5 to 5% by weight, based on the total weight of the composition.

The binder is to prevent the separation of the powder and the liquid component in the dentifrice, and may be a combination of a cellulose derivative such as sodium carboxymethylcellulose, methylcellulose or hydroxypropylcellulose, sodium alginate, carrageenan or xanthan gum alone or in combination 0.1 to 5% by weight, preferably 0.3 to 2% by weight, of the total weight of the composition is used.

The abrasive is used to remove calcium oxide (CaCO ₃ ), dicalcium phosphate (CaHPO ₄ , CaHPO ₄ 2H ₂ O), silicic anhydride (SiO ₂ ) ₂ H ₂ O), aluminum hydroxide (Al (OH) ₃ ), potassium pyrophosphate, magnesium carbonate, etc. are used alone or in admixture of two or more kinds to form a composition in an amount of 1 to 60 wt%, preferably 10 to 50 wt% use.

The flavor agent is used for imparting an exhilarating feeling and smell to the toothpaste to enhance the sensation of use. The flavorant is used in an amount of 1 to 60% by weight, preferably 0.01 to 10% by weight, based on the total weight of the composition of peppermint oil, spearmint oil, menthol, 5% by weight.

The sweetener is used to remove an unpleasant taste or raw material from a toothpaste raw material and to improve a cooling sensation. The sweetener is used in an amount of 1 to 60% by weight, preferably 1 to 60% by weight of the total composition of saccharin acid, aspartame, xylitol, 0.01 to 5% by weight is used.

The active ingredient is for the purpose of prevention of tooth decay, prevention of periodontal disease, prevention of gingivitis, removal of bad breath, and the like. Sodium fluorophosphate, or the like, or a mixture of two or more thereof. In the present invention, a fermented extract of Rhododendron is used as a pharmaceutical active ingredient.

The oral composition of the present invention may be used alone or in combination, or may be used in combination with other oral compositions other than the present invention.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .

Manufacturing example  1. Preparation of Fermented Extract

The shrubs were shredded three times for 5 hours with 70% (v / v) aqueous ethanol solution, cooled down, and filtered through Whatman # 4 filter paper. The filtered extract was concentrated under reduced pressure and freeze-dried at 50 DEG C or lower.

The microbial culture medium was prepared by adding YPD medium (1% yeast extract, 2% polypeptone, 2% D-glucose / 1 L H2O) and 1 to 30% by weight, preferably 10% Respectively.

The medium was supplemented with Saccharomyces < RTI ID = 0.0 > cerevisiae ATCC 18824) was inoculated at 100,000 ~ 10,000,000 cfu and cultured at 30 ~ 37 ° C for 12 ~ 72 hours.

After the culture, the culture was centrifuged to remove the bacteria and the fermentation product was obtained. Ethanol was added thereto to a final concentration of 70% (V / V), followed by refluxing three times for 5 hours. After cooling, Filtered through Whatman # 4 filter paper. The filtered fermented extract was filtered with 0.25 μm filter paper, and then concentrated under reduced pressure and freeze-dried at 600 ° C or less. The resulting crude extract or lyophilized product was dissolved in at least one solvent selected from purified water, ethanol and butylene glycol so as to contain 0.001 to 70% by weight of the concentrate to obtain a fermented extract.

Comparative Manufacturing Example  1. Horsetail extract

As a comparative example of the fermentation extract of Rhus verniciflua, Rhus verniciflua extract was prepared. The rhizome extract was collected by refluxing three times for 5 hours in a 70% (v / v) aqueous ethanol solution, shaking, and filtered through Whatman # 4 filter paper. The filtered extract was concentrated under reduced pressure and freeze-dried at 50 DEG C or lower.

Experimental Example  1. Inhibitory Effect of Fermented Extracts on the Growth of Oral Microorganisms

There are many microorganisms in the mouth and most of them are harmless or symbiotic microorganisms, but specific microorganisms are microorganisms that harm oral health such as dental caries, periodontal diseases and bad breath. In Experimental Example 1, the effect of the fermentation extract obtained from Preparation Example 1 on the growth of oral microorganisms was measured. The microorganisms used for the test were Streptococcus mutans ( Streptococcus mutans) mutans ATCC 25175), Actinomyces < RTI ID = 0.0 > viscosus ATCC 15987) as the causative agent of bad breath, Fusobacterium < RTI ID = 0.0 > nulcleatum ATCC 25586) and Porphyromonas gingivalis (ATCC 33277) were cultivated as Actinobacillus actinobacillus actinomycetemcomitans ATCC 29522) was used. The antimicrobial effect was visualized by paper disc method.

 50 μg of fermentation extract of Rhus japonica melted in 50% ethanol at a concentration of 100 mg / ml was dripped onto a paper disk (Toyo Roshi Kaisha, 8 mm diameter) and dried. Then, the disk was placed on the agar medium containing 0.1 ml of the strain, and cultured in an anaerobic chamber at 37 ° C for 4 to 5 days. Then, the size of the inhibitory zone was measured to confirm the antimicrobial activity. The results are shown in Table 1, and the conditions for culturing each microorganism are shown in Table 2.

Strain name  Size of inhibition zone (mm) Production Example 1 Comparative Preparation Example 1 Streptococcus mutans ATCC 25175 21 16 Actinomyces viscosus ATCC15987 19 13 Fusobacterium nulcleatum ATCC 25586 17 13 Porphyromonas gingivalis ATCC 33277 22 15 Actinobacillus actinomycetemcomitans ATCC 29522 19 16

Strain Culture conditions Classification Strain name Temperature badge gram positive Streptococcus mutans
ATCC 25175 37˚C BHI Actinomyces viscosus ATCC15987 37˚C Modified chopped meat medium gram negative Fusobacterium nulcleatum ATCC 25586 37˚C Modified chopped meat medium Porphyromonas gingivalis ATCC 33277 37˚C TSA Hemin Menadione medium Actinobacillus actinomycetemcomitans ATCC 29522 37˚C BHI

As shown in Table 1, it was confirmed that Production Example 1 exhibited antibacterial effect on Streptococcus mutans ATCC 25175 and Actinomyces viscosus ATCC 15987, causative bacteria of dental caries, Was superior to Comparative Production Example 1. The bad breath causative organism, Fusobacterium nulcleatum ATCC 25586), Porphyromonas gingivalis ATCC 33277, and it was confirmed that Production Example 1 has an antibacterial effect, which is superior to Comparative Production Example 1. In addition, it was confirmed that Production Example 1 exhibited antibacterial effect on Actinobacillus actinomycetemcomitans ATCC 29522 as a periodontal disease causative organism, and it was confirmed to be superior to Comparative Production Example 1. Therefore, it can be seen that the ferret extract of the present invention has an oral microbial growth inhibitory effect.

Experimental Example  2. Inhibitory Effect of Fermentation Extracts from Rice

Streptococcus mutans, an anaerobic bacterium in the oral cavity, forms the plaque and decomposes the sugar to produce organic acids such as lactic acid, thereby lowering the pH. This causes dental caries by demineralizing the enamel of the tooth. Therefore, it is necessary to suppress acid production to prevent dental caries. In Experimental Example 2, the effect of inhibiting acid production by Streptococcus mutans in the fermentation extract of Opuntia ficus-indica obtained in Preparation Example 1 was measured.

The acid production inhibitory effect of the ferret extract was measured by the method of confirming the pH of the Streptococcus mutans culture solution. First, Preparation Examples 1 to 4 were added to the brain medium (Brain heart infusion) liquid medium (Kisan bio, Korea) containing 1% sucrose at various concentrations, and Streptococcus mutans was inoculated into a 24 Lt; / RTI > A portion of the culture was sampled immediately after and 24 hours after the fermentation, and the pH was measured using a pH meter (Thermoscientific, USA).

The acid production inhibitory effect of the fermented extract of Opudia is shown in Table 3.

sample density pH Before culture After 24 hours of incubation Production Example 1 1,000ppm 7.20 6.39 5,000 ppm 7.18 6.98 10,000ppm 7.19 7.01 Comparative Preparation Example 1 1,000ppm 7.15 6.05 5,000 ppm 7.21 6.24 10,000ppm 7.20 6.49 Chlorhexidine 1,000ppm 7.19 7.15 ethanol 10,000ppm 7.18 5.24

As shown in Table 3, it was confirmed that the culture medium of Streptococcus mutans containing the ferret extract preparation of Ferret Extract almost retained the initial pH 24 hours after the fermentation. It was confirmed that the effect of inhibiting acid formation was superior to that of Comparative Preparation Example 1 and that the acid generation inhibitory effect was similar to that of positive control group, chlorhexidine gluconate (sigma, USA). Therefore, it can be seen that the horseradish fermentation extract of the present invention has an effect of inhibiting acid production.

Formulation Example  1. Preparation of oral composition containing fermented extract

A dentifrice composition containing a fermented extract of Opium peach according to Preparation Example 1 was prepared and a dentifrice composition containing cetylpyridinium chloride as an active ingredient in place of the fermented extract of Opium peach Example 1 A dentifrice composition (Comparative Formulation Example 2) containing hydrogen peroxide as a tooth whitening ingredient and an oral composition (Comparative Formulation Example 3) containing neither a fermented extract and an active ingredient are shown in Table 4 below.

(Unit: wt%) division ingredient Formulation Example 1 Formulation Example 2 Comparative Formulation Example 1 Comparative Formulation Example 2 Comparative Formulation Example 3 Wetting agent Concentrated glycerin 5 5 5 5 5 Sorbitol solution 10 10 10 10 10 Binder Carboxymethylcellulose sodium One One One One One Surfactants Sodium lauryl sulfate 2 2 2 2 2 abrasive Precipitated silica 20 20 20 20 20 Sweetener Sodium saccharin 0.2 0.2 0.2 0.2 0.2 Buffer Sodium phosphate monobasic 0.1 0.1 0.1 0.1 0.1 Sodium tertiary phosphate 0.05 0.05 0.05 0.05 0.05 Active ingredient Production Example 1 0.5 1.0 - - - Cetylpyridinium chloride
- - 0.5 - - Sodium fluoride 0.3 0.3 0.3 0.3 0.3 Hydrogen peroxide (30%) - - - 3 - Flavor 0.5 0.5 0.5 0.5 0.5 Purified water Remainder Remainder Remainder Remainder Remainder total 100 100 100 100 100

Experimental Example  3. Inhibitory Effect on the Acid Production of Dentifrice Composition Including Fermented Extracts

Experimental Example 3 was conducted to examine the effect of inhibiting acid production of dentifrice compositions according to Formulation Examples 1 to 2 and Comparative Formulation Examples containing fermentation extract obtained from Preparation Example 1. For this purpose, 60 subjects were recruited . After stopping the oral environment management for 24 hours, the subjects measured the oral pH using a compact portable pH meter (Horiba scientific, Japan). Two groups of 20 individuals were made so that the average value of oral pH was similar. Formulation 1 was given to one group, Formulation Example 2 to Group 2, and Comparative Formulation Example 3 to Group 3. The pH of each group was measured after 6 hours after rinsing with the use of Formulation Example 1 or Formulation Example 2 or Comparative Formulation Example 3 for each group. Table 5 shows the inhibitory effect on the acid formation according to the use of the formulation examples and the comparative formulation examples.

Elapsed time Pre-experiment pH After 6 hours pH Formulation Example 1 5.5 6.2 Formulation Example 2 5.3 6.7 Comparative Formulation Example 3 5.5 5.7

As shown in Table 5, the acid production inhibitory effect of the dentifrice compositions of Formulation Examples 1 to 2 including the fermentation extract of Opium larvae was remarkably reduced compared to that before the experiment. Therefore, it can be seen that the oral composition containing the ferret extract of the present invention has an effect of inhibiting acid production.

Experimental Example  4. Removal of bad breath from dentifrice composition containing fermented extract

Experimental Example 3 was conducted to examine the effect of removing the bad breath of the dentifrice composition according to Formulation Examples 1 and 2 and Comparative Formulation Examples 1 and 3 containing the fermented extract obtained from Preparation Example 1, Eighty patients without oral and systemic diseases were recruited. After halting oral care for 48 hours, halitosis was measured using Halimeter (Interscan Co., USA) and divided into 4 groups of 20 individuals each with a mean value. Formulation 1 was provided for Group 1, Formulation Example 2 for Group 2, Comparative Formulation Example 1 for Group 3, and Comparative Formulation Example 3 for Group 4. For each group, mouth rinsing was carried out using Formulation Example 1, Formulation Example 2, and Comparative Formulation Example 1 or 3, and after 30 minutes, halitosis degree was measured with a halimeter. After 4 weeks, toothpaste was soaked 3 times a day, and it was summoned for 4 weeks at intervals of 2 weeks, and the degree of bad breath was measured 30 minutes after brushing. The effects of reducing the amount of bad breath according to the four-week use period of the formulation examples and the comparative formulation examples are shown in Table 6 below.

Elapsed time Immediately after the experiment 2 weeks 4 weeks Formulation Example 1 218 151 115 Formulation Example 2 216 139 104 Comparative Formulation Example 1 219 131 101 Comparative Formulation Example 3 222 201 193

As shown in Table 6, the odor scores of the dentifrice compositions of Formulation Examples 1 and 2 including the fermentation extract of Opium larvae were remarkably decreased as compared with immediately after the experiment. It was confirmed that similar effect was obtained compared with Comparative Formulation Example 1 which is a dentifrice composition containing cetylpyridinium chloride as an active ingredient for removing bad breath. Therefore, it can be seen that the oral composition containing the ferret extract of the present invention has the effect of removing bad breath.

Experimental Example  5. DENTAL DISEASE REDUCTION EFFECT OF DENTAL COMPOSITIONS CONTAINING HORIBA FERMENTATION EXTRACTS

Experimental Example 5 was conducted to investigate the effect of the dentifrice compositions according to the formulation examples and comparative formulations containing fermentation extracts obtained in Preparation Examples 1 and 2 on ginseng inflammation relief. For this purpose, 80 men and women suffering from ginseng inflammation . The subjects were divided into four groups of 20 persons so that there was no significant difference in the mean values between the limb gingiva and the nipple gingiva according to the Loe & Silness gingival index evaluation criteria as shown in Table 7. The first group is provided with Formulation Example 1, the second group with Formulation Example 2, the third group with Comparative Formulation Example 1, and the fourth group with Comparative Formulation Example 3, The experiment was conducted for 3 months. During the test period, any oral care that could affect the oral condition was forbidden. At three months, scores were recorded according to the gingival index evaluation criteria of Table 7 below, and the results were as shown in Table 8 below.

score Contents 0 point Normal 1 point Mild gingivitis (gingiva with slight change in gingival tone and slight swelling but mild inflammation that does not bleed with mild irritation) 2 points Gingivitis with moderate to severe gingivitis (gingivitis, gingivitis that manifests symptoms of swelling and inflammation to the extent that bleeding occurs even with mild stimuli) 3 points High grade gingivitis (gingivitis with marked redness and swelling and severe inflammation that may have ulcers and cause natural bleeding)

Elapsed time Initial value 2 weeks 4 weeks Formulation Example 1 1.57 1.35 1.07 Formulation Example 2 1.52 1.25 0.82 Comparative Formulation Example 1 1.54 1.21 0.75 Comparative Formulation Example 3 1.56 1.54 1.52

As shown in Table 8, the gingival index of the dentifrice compositions of Formulation Examples 1 and 2 including the fermented extract of Opuntia ficus significantly decreased after 4 weeks, as compared with that immediately after the experiment. Whereas Comparative Formulation Example 3, which did not contain the fermented extract, showed no gingivitis mitigating effect at all. Therefore, it can be seen that the oral composition containing the ferret extract of the present invention has an effect of alleviating oral diseases.

Experimental Example  6. Tooth whitening effect of dentifrice composition containing fermented extract

Experimental Example 6 was conducted to examine tooth whitening effect of the dentifrice composition according to the formulation example and the comparative formulation example including the ferret extract prepared in Preparation Example 1, and 80 teeth with comparatively pressed teeth were recruited for the experiment . Before starting the experiment, the hue of the incisal surface of the subjects was measured by a Trubyte bioform colorimeter in a room with a constant lighting device, and the hue was measured and classified into four groups each having an average value of approximately 20 . Formulation 1 was given to Group 1, Formulation Example 2 was given to Group 2, Comparative Formulation Example 2 was given to Group 3, and Comparative Formulation Example 3 was given to Group 4, The experiment was conducted for 2 months. During the test period, food was consumed according to usual eating habits, but on the day of measurement, jade surface, coffee, cola and cigarette were prohibited. On the day of measurement, tooth color was measured 30 minutes after brushing. The increase of tooth brightness was selected from 10 kinds of sensory identification among 24 kinds of standard of brightness of TruBite biofom color system, and the score was given from 1 point to 10 points according to brightness. The tooth whitening effect was evaluated by evaluating the brightness value after brushing for 2 months, and then by increasing the average brightness value with respect to the brightness value before the experiment. The tooth whitening effect according to the period of use for two months in the formulation examples and comparative formulation examples is shown in Table 9 below.

Elapsed time Initial value 1 month 2 months Rate of increase in brightness after 2 months (%) Formulation Example 1 4.5 5.1 5.8 28.8 Formulation Example 2 4.6 5.8 7.1 54.3 Comparative Formulation Example 2 4.4 5.7 7.0 59.1 Comparative Formulation Example 3 4.6 4.7 5.0 8.6

As shown in Table 9, when the lightness of the teeth after 2 months was observed, the rate of increase in lightness of teeth of the dentifrice compositions of Formulation Examples 1 and 2 including the fermentation extract of Opium larvae was remarkably improved as compared with the initial value. Similar whitening effect to Comparative Formulation Example 2 containing hydrogen peroxide as a whitening active ingredient was observed, whereas Comparative Formulation Example 3 containing no fermented extract or active ingredient showed no whitening effect at all. Therefore, it can be seen that the composition for oral use containing the fermented extract of Opuscera of the present invention has a tooth whitening effect.

The present invention has been described with reference to the preferred embodiments. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Therefore, the disclosed embodiments should be considered in an illustrative rather than a restrictive sense. The scope of the present invention is defined by the appended claims rather than by the foregoing description, and all differences within the scope of equivalents thereof should be construed as being included in the present invention.

Claims (9)

An oral composition having an oral microbial growth inhibiting effect,
The present invention relates to a fermented milk extract obtained by fermenting Saccharomyces cerevisisae ATCC 18824 as an active ingredient,
The oral microorganism may be selected from the group consisting of Streptococcus mutans , Fusobacterium nulcleatum , Porphyromonas gingivalis , Actinomyces viscosus , Actinobacillus sp. Actinobacillus actinomycetemcomitans , or Streptococcus sanguinis , which has an oral microbial growth inhibitory effect. delete delete The method according to claim 1,
Wherein the oral composition has an effect of inhibiting acid production and preventing caries. The method according to claim 1,
Wherein the composition for oral cavity has a bad breath removing effect. The method according to claim 1,
Wherein the oral composition has an oral disease-preventing effect. A composition for oral use having a tooth whitening effect, comprising as an active ingredient a fermented extract obtained by fermenting Saccharomyces cerevisisae (ATCC 18824). 8. The method according to any one of claims 1 to 7,
Wherein the fermented extract of Opium is contained in an amount of 0.0001 to 30.0% by weight based on the freeze-dried weight based on the total weight of the oral composition. 8. The method according to any one of claims 1 to 7,
Wherein the oral composition is contained in an oral product selected from the group consisting of a toothpaste, a mouthwash, an oral cleanser, a gum, a candy, an oral spray, an oral ointment, a mouthwash and a mouthwash.