KR20050078923A - Toothpaste composition for prevention against dental caries and periodontal disease - Google Patents

Abstract

The present invention relates to a toothpaste composition for preventing dental caries and periodontal disease, the toothpaste composition mainly comprising a tooth abrasive, tooth polish and fluoride preparation, extracts obtained from gall, green tea, pagoji or salvia Provided is a toothpaste composition for preventing dental caries and periodontal disease, wherein the mixture contains 0.1 to 0.8% by weight of the total composition.

The toothpaste composition of the present invention may be useful for preventing dental caries and periodontal disease by containing herbal extracts that are effective in inhibiting plaque formation and have no side effects on the human body.

Description

Toothpaste Composition for Prevention against Dental Caries and Periodontal Disease}

The present invention relates to a toothpaste composition for preventing dental caries and periodontal disease, and more particularly contains natural extracts such as gallastia, green tea, pagoji or salvia and effective in suppressing plaque formation but do not have side effects to human body dental caries and periodontal disease It relates to a prophylactic toothpaste composition.

Toothpaste basically performs tooth surface cleaning and tooth polishing. In the past, it was mainly expected to enhance the cosmetic function of teeth by these basic actions. In recent years, toothpaste has increased due to the increasing public interest in oral health. Various efforts have been made by dentists and toothpaste manufacturers to prevent the disease, and extensive research has been carried out to develop special detergents containing substances that prevent and treat oral diseases. According to this trend, researches on antimicrobial agents that can inhibit microorganisms causing dental caries and periodontal disease have been actively conducted.

Antimicrobial agents for the prevention of dental caries have the functions of reducing plaque, suppressing new plaque formation, selective suppression of specific bacteria related to disease and suppressing expression of toxic determinants. Additives used in toothpaste to suppress plaque formation include metal salts, positive charges, nonionic phenols, enzymes, peroxides, sugar substitutes, surfactants, and natural substances. Among these substances, they have strong antibacterial properties alone, but when added to toothpaste for a long time, their stability problems are not completely solved, such as reduced oral effects due to oral ecological destruction, tooth coloring, or reaction with other ingredients in toothpaste. There are many kinds. However, toothpaste is used continuously in parallel with brushing, so there should be little side effects on the human body. In view of this, plant natural extracts that can replace the existing chemical synthetic antimicrobial substances added to toothpaste for the prevention and treatment of dental caries and periodontal disease can be used safely and continuously without side effects while effectively removing bacteria in the oral cavity. Research on antimicrobial agents is continuing.

In particular, many plants are interested in research on these herbs because the plants used as herbal medicine in the herbal medicine has been proven to be effective in clinical and easy access to research on the development of antimicrobial efficacy and bioactive substances. In the book, herbal medicines are one of the methods that have been studied since ancient times, proving that drugs extracted from herbal medicines are safe for humans, and that their efficacy and effects are persistent. There are scientific approaches to the component fractions and their effects on various herbal preparations that have been passed beyond the limitations of empirical oriental medicine, and some have made significant progress and have reached the level of clinical use. These herbal preparations are considered to be applicable drugs in view of the economics for practical application with the stability which has been proven for some time in the past.

Kang et al. Reported that medicinal herbs containing medicinal herbs were effective in inhibiting plaque (Kang, In-Chul, Sang-Cheol Lee, Jong-Pyung Jung, Sung-Hee Son. A study on the clinical and microbiological effects of toothpaste containing green tea, myrrh, and baekhwi Riding extract. Korean Journal of Periodontal Science 1991; 21: 1-15). Park et al. Reported that Hwangbaek, Gilgyeong, hyunggwa extract and bamboo salt were used in the treatment of plaque and had the effect of inhibiting plaque (Park Kyung-il, Choi Yu-jin. A study on the effect of the medicinal herbs containing medicinal herbs on plaque and gingivitis. 1996; 18 (2): 301-310.). Among plant extracts, it inhibits the growth of mutans streptococci , the carnivorous causative organism, in particular oral fungi, which is a gall bladder, pagoji, spectators, red ginseng, elm tree, black pepper, cinnamon, control, maddu, clove, cone, generalize, it is reported the like noksucho, Scrophulariaceae, green tea, Sophora flavescens, Schisandra chinensis (yiganguk, yisugyeong, janggiwan. cinnamon extract-free Tansu Streptococcus seed (mutans growth and the effect on the adhesion to the HA beads (hydroxyapatite beads) of streptococci) Korean Journal of Oral Health 1999; 23 (1): 25-33. In addition, Schizandra chinensis, T. gourd and Ginkgo biloba extracts have antibacterial effects on the causative agents of periodontal disease (Kim Tae-il, Hye-ri Yeong, Ryu In-chul, Bae-hwan, Jung Jong-pyung. Research Korean Journal of Periodontology 1996; 26 (2): 542-555). In addition, the gall bladder is a word that refers to insect lumps caused by insects parasitic on red leaf stems and new leaves. It has been used for gastric ulcer, duodenal ulcer, gastritis and stomatitis for a long time. Lee et al. Have reported relatively broad antimicrobial spectrum of gall extract and their potential as a natural food preservative (Lee Young-cheol, Se-wook Oh and Hee-do Hong. Antimicrobial properties of edible medicinal plant extracts. Korean Journal of Food Science and Technology 2002; 34 (4): 700-709).

Conventionally, generally ointment toothpaste compositions have been prepared by appropriately adding main ingredients of dentifrice abrasives, detergents, humectants, binders and fluorine agents. In this case, the teeth are polished and cleaned by the main raw material of the tooth polishing agent and the detergent, so that the plaque accumulated on the teeth can be removed.

At this time, if the toothpaste containing the antimicrobial substance will be effective toothpaste clean. Until now, research and product application for the development of natural materials for the use of toothpaste, which are not known to be contained in toothpaste, are in progress.

As such, it is necessary to continue to search for beneficial antimicrobial substances and confirm their effectiveness to expand their use, and the antimicrobial substances are greatly increased by the synergist, so they try to apply them to microorganisms with these studies. Needs to be. In addition, the search for herbal medicines showing antimicrobial activity and the development of detergent using them are considered to be important.

Accordingly, an object of the present invention is to find a natural substance that can inhibit plaque formation without side effects, and to provide a dentifrice composition containing a herbal extract that is effective in suppressing plaque formation and has no side effects on the human body.

According to the present invention, in the toothpaste composition based on the toothpaste abrasive, the detergent and the fluorine agent, at least one or a mixture of the extracts from the gall bladder, the green tea, the pagoji or the salvia is in an amount of 0.1-0.8% by weight based on the total composition. Toothpaste composition for preventing dental caries and periodontal disease is characterized by containing.

Hereinafter, the present invention will be described in detail.

The present inventors have studied with various natural extracts to develop a natural extract that can be used continuously without any side effects or to replace the chemical antibacterial substances added to the toothpaste for the prevention of dental caries and periodontal disease. The extract obtained from the gall bladder, pagoji, green tea, salvia, etc. is effective in inhibiting the formation of artificial plaque, but also has no side effects to the human body to complete the present invention.

Natural extracts contained in the toothpaste composition of the present invention is a gallengja, green tea, pagoji, Salvia extract, such natural extract is included at least one kind or mixed. These extracts are contained in an amount of 0.1 to 0.8% by weight based on the total composition, showing an excellent plaque suppression effect within this range.

Among the natural extracts, it is most preferred to use the gall bladder extract, which is preferably contained in an amount of 0.1-0.4% by weight based on the total composition, and most preferably in an amount of 0.4% by weight.

The cause of dental caries and periodontal disease is in plaque, so in order to find natural substances that can suppress plaque formation without side effects, the present invention measured the inhibitory effect of artificial plaque formation on various kinds of natural extracts and also tested the toxicity of human cells. In parallel, we selected herbal extracts that were effective in suppressing plaque formation but had no side effects on the human body. Finally, the toothpaste added with these natural extracts was examined to see if they were clinically effective in vivo.

Hereinafter, the present invention will be described in more detail with reference to the following examples.

Example 1. Measurement of inhibitory effect of artificial plaque formation of herbal extracts

end. Extraction Method

Five times (v / w) per 100 g of sample was immersed in 80% MeOH, and extracted three times while standing at room temperature for 24 hours, followed by filtration (Whatman No. 2). The filtrate was concentrated under reduced pressure with a vacuum evaporator (TYPE N-2N, Eyela, Tokyo, Japan) equipped with a cooling aspirator (COOL ACE CA-111, Eyela, Tokyo, Japan) and then lyophilized (FDU-500, Eyela, Tokyo). , Japan) and used as a sample (see FIG. 1).

I. Disclosure strains and cultures

Streptococcus mutans serotype c (Ingbritt strain) was disclosed, the culture was inoculated in M17 culture solution (Difco, Detroit, MI. USA) by lyophilization and incubated at 37 ℃ in a carbon dioxide incubator.

All. Inhibition of artificial plaque formation

0.5% yeast extract, 5% sucrose, and 0.1M MOPS buffer (3- (N-Mor polyno) propanesulfonic acid) were added to M17 cultures at final concentrations of 0.1%, 0.4% and 0.8%. Gallja (Gala Rhois ), Green tea ( Camallia sinensis ), Pagoji ( Psoralea corylifolia ), Salvia ( Salvia miltiorrhiza ), Spectators (Dry Opteris Crassirhizoma ( Dryopteris Crassirhizoma )) and Gilkyung ( Platicodon grandiflorum ) extracts were added to prepare a 40 ml beaker.

Inoculation of 5.0 × 10 ⁶ S. mutans and the method described in the report of Songdeung for the measurement of plaque weight (Song Do-won, Yang Kyu-ho, Jung-jin, Oh Jong-seok. Artificial plaque suppression by mutanase produced by Streptomyces exfoliatus. 1997; 24: 449-455), three 0.016 inch stainless steel calibration wires (ORMCO, Glendora, CA, USA) were hung in a beaker and submerged in medium. After shaking for 24 hours while shaking (30rpm) in a 37 ℃ shaking incubator, the weight of the artificial plaque formed on the three wires was measured nine times. The control group was prepared by adding 0.1M MOPS to M17SY (5% sucrose and 0.5% yeast extract) without adding the herbal extract, and inoculating and incubating S. mutans at 5.0 × 10 ⁶ cfu / ml.

la. Measurement result of artificial plaque formation inhibitory effect of herbal extracts

Results of measuring the artificial plaque formation inhibitory effect of the herbal extracts are shown in Table 1 and FIG.

TABLE 1

Concentration (% by weight) gallnut green tea Pagoji Salvia crowd Street view Control 0.1 26.75 ± 9.05 119.73 ± 17.71 121.28 ± 42.30 119.82 ± 36.24 97.22 ± 13.45 151.15 ± 39.57 136.52 ± 18.40 0.4 1.47 ± 1.06 55.87 ± 9.26 3.01 ± 1.52 86.57 ± 16.66 81.16 ± 28.30 129.23 ± 16.20 0.8 1.31 ± 1.16 3.43 ± 1.39 2.15 ± 1.58 74.95 ± 24.38 57.90 ± 18.65 202.07 ± 40.53

Fivefolds reduced artificial plaque formation by 5 times to 26.75 ± 9.05 in 0.1% and completely inhibited 1.47 ± 1.06 and 1.31 ± 1.16 in 0.4% and 0.8%.

Green tea did not inhibit artificial plaque formation from 0.1% to 119.73 ± 17.71 and decreased 2.4 times from 0.4% to 55.87 ± 9.26, but almost completely inhibited from 0.8% to 3.43 ± 1.39.

In 0.1% of cases, phage was 121.28 ± 42.30, which did not completely inhibit artificial plaque formation, but 0.41 and 0.8% completely inhibited artificial plaque formation by 3.01 ± 1.52 and 2.15 ± 1.58.

Saliva was not suppressed at 119.82 ± 36.24 in 0.1%, decreased 1.6 times to 86.57 ± 16.66 in 0.4% and 1.8 times to 74.95 ± 24.38 in 0.8%.

The spectral weight was very weak at 0.1% in 97.22 ± 13.45, which was 1.7 times lower in 81% to 81.16 ± 28.30 and 2.4 times in 0.8%, 57.90 ± 18.65.

Kil-kyung increased artificial plaque formation to 151.15 ± 39.57 at 0.1%, but only slightly reduced it to 129.23 ± 16.20 at 0.4% and 202.07 ± 40.53 at 0.8%.

Example 2 Cytotoxicity Measurement of Herbal Extracts

end. Sample preparation

Samples were dissolved in dimethylsulfoxide (DMSO) (Sigma, USA) and diluted in medium without serum to make final chemical concentrations of 0.0004%, 0.0002% and 0.0001%, respectively.

I. Cell and culture

Gingival fibroblasts (hGFs) were collected from healthy adults who visited Chonnam National University Hospital. Alpha-supplemented gentamicin (10 mg / ml, JBI, Korea) and 10% fetal bovine serum (FBS) (GibcoBRL, UK) in an incubator at 37 ° C and 5% CO ₂ humidified with scissors. After culturing in the minimum essential medium, the medium was changed to a new one, and adhesive fibroblasts were allowed to collect for up to 4 days.

Cells were then trypsinized with trypsin-EDTA solution (GibcoBRL, UK) and re-cultured to use cells cultured between the third and eighth.

All. Cell viability by MTT reduction assay

Colorimetric analysis of MTT [3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyl tetrazolium bromide] (Sigma, USA) provides a measure of cell respiration by measuring the dehydrogenase activity of succinic acid. Fibroblasts were seeded in 96-well plates at a density of ³ × 10 ³ cells / well in 0.1 ml medium and incubated in medium at 37 ° C. for two days. Labeled concentration samples (0.0004%, 0.0002%, 0.0001%) were added to each well, and the cells were incubated at 37 ° C. for one day and MTT was added to the medium for 4 hours at 37 ° C.

Reactants of MTT were extracted from DMSO and the optical density was indicated by blanking DMSO using an ELISER reader (ELx800uv, BIO Tek Instruments, Inc.) at 570 nm and then converted to a control to express the viability of the cells.

hemp. Cytotoxicity of Herbal Extracts

The results of measuring the cytotoxicity of the herbal extracts through the above method are shown in Table 2 and FIG. 2.

TABLE 2

Concentration (% by weight) gallnut green tea Pagoji Salvia crowd Street view 0.0001 97.23 ± 1.66 101.98 ± 1.01 98.17 ± 1.46 100.66 ± 0.26 90.30 ± 0.67 98.81 ± 0.79 0.0002 108.28 ± 0.88 99.5 ± 1.19 102.61 ± 1.10 102.36 ± 0.27 91.08 ± 0.86 101.05 ± 0.77 0.0004 107.15 ± 1.31 104.32 ± 0.71 103.60 ± 1.34 104.29 ± 0.49 89.73 ± 0.94 101.64 ± 0.66 Control 100 ± 1.15 100 ± 0.66 100 ± 1.81 100 ± 0.59 100 ± 2.43 100 ± 1.46

In the cytotoxicity test treated with gingival fibroblasts with herbal extracts, the viability of the gingival fibroblasts treated with gall bladder, pagoji, and gilyeong extracts decreased the cell viability in the 0.0001% extract, whereas % And 0.0004% increased cell survival. Green tea was also increased at 0.0001% and 0.004%. However, gingival fibroblasts treated with 0.0001%, 0.0002% and 0.0004% concentrations showed decreased cell survival. Therefore, except for spectators, gallnut, pagoji, green tea, red ginseng, and gilyeong extracts did not reduce the viability of gingival fibroblasts.

Example 3. Clinical Trials

end. Composition of Experimental Toothpaste

In the present experiment, four experimental toothpastes were used to investigate the effect of plaque formation when the extract was added to the toothpaste. In other words, Periogum Care, a commercially available toothpaste with fluoride (NaF) that is clinically recognized as a negative control, is a toothpaste that does not contain fluoride and extracts ^. (LG corp) was used as a positive control and 0.4% was used in the toothpaste diluent of the five extracts of natural extracts 5 and pagoji. Toothpaste composition used in this clinical study is shown in Table 3.

[Table 3] Composition of Experimental Toothpaste

Test group (all silica base) Active ingredient (% by weight) NaF Natural extracts Negative Control Toothpaste - - Ginseng Extract Toothpaste (Invention Example 1) - Galla Rhois 0.4 Pagoji Extract Toothpaste (Invention Example 2) - P.oralea corylifolia 0.4 Positive Control Toothpaste (Periogum Care ^? ) 0.2 -

I. Survey subject

Among the volunteers who agreed to participate in the experiment, there were 35 patients who did not have specific symptoms in general health, relatively even dentition, and no history of medication in the last 3 months, and had less than 1 oral prosthesis and no periodontal disease. It was. The participants in this clinical study were college students in their 20s and 13 males and 22 females.

All. Survey method

Subjects were randomly divided into groups 1, 2, 3, and 4 by measuring plaque index, followed by double-blind and crossover experiments. Subjects in each group were given 5.0 g of toothpaste diluted in 15 ml of distilled water (hereinafter referred to as dentifrice diluent) and optionally corded for 4 days during the clinical study. During the 4 days of the study, 15 ml of the solution was used twice a day for 1 minute, and the same container was supplied so that each toothpaste diluent could be used in a situation in which both the experimenter and the subject could not distinguish.

On the first day, after the dental floss treatment including tartar removal, all of the same sized medium-strength toothbrushes and general toothpastes without pharmaceutical ingredients were distributed. For a week, they were individually trained on how to use toothbrushes to use the toothpaste, which is free of pharmaceutical ingredients, by brushing with the rotary method.

One week later, a retest was carried out and a scrubbed scrubbed was used to create an oral environment in which all subjects had the same conditions. The toothpaste (Invention Examples 1 and 2) of the present invention was distributed after recovering the general toothpaste and toothbrush which had already been distributed. The subjects were brushed for 1 minute with 15 ml of toothpaste diluent twice daily for 4 days, and dental treatment and brushing and flossing were prohibited during the four crossover experiments.

The plaque index was measured before and at the beginning of the experiment. To determine the degree of plaque attached to the buccal surface of maxillary right second premolar and canine, maxillary left first molar, mandibular left first molar, mandibular left incisor and mandibular right first premolar after using coloring agent. The plaque index was scored according to the attachment score standard of Hygiene Performance. The evaluation criteria are divided into 5 parts of mesial part, centrifugal part, gingival part, center part, and cutting part respectively. If the bacterial film is not attached to each part, it is scored as 0. If the bacterial film is attached, it is 1 point. To be rated. The average value calculated by evaluating the degree of plaque attached to the tooth surface was used as the individual plaque index.

On the last day of each trial period, all subjects underwent dental scrubs along with plaque index measurements, and then distributed the same standard-strength toothbrushes and generic toothpastes without any pharmaceutical ingredients, followed by a 10-day rest period. It was restored to its original state. Four crossover experiments were conducted in the same manner to determine the plaque index.

la. Statistical processing of results and significance test

Statistical analysis method for the experimental results was performed using the statistical analysis program SPSS 10.1, and a corresponding sample T-test was performed to find out the significance of each test score.

hemp. Plaque formation inhibition result

As a result of measuring the plaque index of the study subjects through the above method, the plaque index of the group using the five-blade toothpaste was 2.93 ± 0.55, the positive control toothpaste (Periogum Care) was 2.93 ± 0.46 and the pagoji-containing toothpaste was 3.12 ± 0.45. The negative control toothpaste was 3.21 ± 0.63, and there was a significant difference between the negative control toothpaste, the negative control toothpaste, the positive control toothpaste and the negative control toothpaste. From the above results, in particular, the dentifrice-containing toothpaste had a similar level of effect as the commercially prepared positive control toothpaste (containing NaF, vitamin B6, and sodium lauryl sulfate as a cleaning agent), even though only the quinax extract was added to the silica abrasive. It can be seen that the gall bladder-containing toothpaste has an excellent antibacterial effect and inhibiting plaque formation.

The toothpaste composition of the present invention may be useful for preventing dental caries and periodontal disease by containing herbal extracts that are effective in inhibiting plaque formation and have no side effects on the human body.

Figure 1 shows the extraction process of the various herbal extracts used in this embodiment,

Figure 2 is a graph showing the measurement results of the artificial plaque formation inhibitory effect of various herbal extracts used in this embodiment,

Figure 3 is a graph showing the cytotoxicity measurement results of various herbal extracts used in this example.

Claims (3)

Toothpaste composition based on dentifrice abrasives, detergents and fluorides, characterized in that it contains at least one or a mixture of ginseng, green tea, pagoji or salvia in an amount of 0.1-0.8% by weight based on the total composition Toothpaste composition for preventing dental caries and periodontal disease. The toothpaste composition for preventing dental caries and periodontal disease, characterized in that the extract is a gall bladder extract. The toothpaste composition for preventing dental caries and periodontal disease according to claim 1 or 2, wherein the extract is contained in an amount of 0.1-0.4% by weight based on the total composition.