KR102058464B1 - Herbal extract composition for gargle - Google Patents

Abstract

The present invention relates to an herbal medicine extract composition for oral and dental gargle. More specifically, the present invention relates to an herbal medicine extract composition for gargle, including a fermentation extract of Nypa fruticans Wurmb as an active ingredient or further including an Asiasari radix ethanol extract, pine needle hydrothermal extract, Portulaca oleracea hydrothermal extract, Ulmi cortex hydrothermal extract, Saururus chinensis fermented extract and propolis ethanol extract in addition to the Nypa fruticans Wurmb fermentation extract.

Description

Herbal extract composition for gargle {Herbal extract composition for gargle}

The present invention relates to a herbal extract composition for oral cavity and dental gargle, which will be described in more detail, comprising fermented extract of hae bamboo shoot (海 竹 荀; 海 竹 荀 (海 竹 荀) as an active ingredient, or in addition to the fermented extract of haeju sprout It relates to Sexin ethanol extract and pine needle hot water extract, purslane hot water extract, milk white skin hot water extract, three hundred seconds fermented extract and propolis ethanol extract composition for gag.

In general, tooth decay and periodontal disease, the most common diseases of teeth, are known to be mainly caused by microorganisms that live in the oral cavity. In other words, oral microorganisms represented by Streptococcus mutans metabolize carbohydrates, especially sugars, glucose, fructose and other sugars contained in food to produce lactic acid as a by-product, and such lactic acid is a component of teeth. It reacts with calcium phosphate to dissolve the enamel, the outer membrane of the tooth, causing tooth decay.

In addition, the Streptococcus mutans form a water-insoluble glucan using a saccharide component, and the glucan is attached to the tooth surface together with other microorganisms in the oral cavity to form a dental plaque or dental calculus. . These plaques and tartars provide a habitat for harmful bacteria in the oral cavity, causing inflammatory reactions in the periodontal tissues, and yellowing discoloration of the teeth, thereby causing damage to dental beauty.

In addition, bad breath in which an unpleasant odor occurs in the oral cavity is caused by food residues remaining in the oral cavity decomposed by microorganisms to produce volatile chemicals. Chemical components causing bad breath include volatile sulfides such as hydrogen sulfide, methyl mercaptan, dimethyl mercaptan, and other volatile amine compounds such as trimethylamine, Aldehydes, fatty acids, ammonia and the like are known.

Therefore, in order to prevent tooth decay and periodontal disease and further suppress bad breath, it is important to first remove plaque, tartar and food residues attached to the teeth through physical brushing. However, this brushing alone does not completely remove the harmful microorganisms inhabiting the oral cavity, so gargle solution is used to maintain a more hygienic oral environment.

Conventionally, gargle solutions used for oral and dental health are mainly antibacterial substances that inhibit the growth of Streptococcus mutans, such as sodium fluoride (NaF), triclosan, chlorhexidine, or antibiotics. Vancomycin, chlorhexidine, spiramycin and the like have been used. However, these chemical goggle solutions cause side effects of vomiting or diarrhea, or the appearance of resistant bacteria, and recently, goggle solutions made of natural herbal extracts that can resolve these side effects have been developed and used.

For example, Patent Registration No. 10-0537834 (December 13, 2005) discloses an oral composition for suppressing bad breath containing the extract of the bark skin as an active ingredient, and Patent No. 10-1017447 (February 2011) On the 17th) is disclosed oral composition and antimicrobial composition for preventing tooth decay containing fennel extract, Patent No. 10-1358507 (January 27, 2014) in the oral cavity containing red ginseng and natural plant-derived extract Compositions for anti-inflammatory, antibacterial and deodorant are disclosed. In addition, the gag solution containing a variety of antimicrobial herbal extract composition as a main component has been introduced, but all of the conventional gag solution is different from the main components and efficacy of the present invention.

On the other hand, with respect to the extract of sea bamboo shoots, one of the main components of the present invention Patent Registration No. 10-1864511 (May 29, 2018) and No. 10-1882657 (July 21, 2018) The cosmetic composition having excellent anti-aging and anti-wrinkle effect while introducing less skin side effects has been introduced by containing the extract of Hakka bamboo shoot as an active ingredient. However, no precedent has been reported for the use of the haejucho extract as a component of the gargle solution.

Patent Registration No. 10-0537834 (December 13, 2005) Registered Patent No. 10-1017447 (February 17, 2011) Registered Patent No. 10-1358507 (January 27, 2014) Patent Registration No. 10-1864511 (May 29, 2018) Patent Registration No. 10-1882657 (July 21, 2018)

An object of the present invention is excellent sterilization effect against oral microorganisms that cause tooth decay and periodontal disease, excellent bad breath and nicotine removal effect, and further excellent taste of the goggle solution and refreshing feeling maintained in the mouth after use It is to provide a new herbal extract composition for gargle.

The herbal extract extract for gargle according to the present invention is characterized in that it comprises the sea cucumber sprout fermentation extract as an active ingredient.

In addition, the herbal extract composition for gargle according to the present invention, the sesame ethanol extract and pine needle hot water extract, purslane hot water extract, milkweed skin hot water extract, triticale fermented extract and propolis ethanol extract each 5 ~ 10 parts by weight of sea bamboo shoot fermentation extract Characterized in that it further comprises 20 parts by weight.

The sea cucumber sprout fermentation extract is put into 3 to 5 times the purified water in the weight ratio of finely sliced sea bamboo shoots in the size of about 5 ~ 10mm, fermented at a temperature of 40 ~ 70 ℃ for about 7 to 8 days, and then removed the solid content And obtained by filtration.

The gargle solution using the herbal extract composition according to the present invention has an excellent bactericidal effect against oral microorganisms causing tooth decay and periodontal disease, and is excellent in bad breath and nicotine removal effects, and furthermore after the taste and use of the gargle solution itself. The refreshing feeling maintained in the mouth has an excellent effect.

The herbal extract extract for gargle according to the present invention comprises a sea cucumber sprout fermentation extract as an active ingredient, sessin ethanol extract and pine needle extract, purslane extract, baekbaekpi extract, three hundred seconds fermentation extract and propolis ethanol extract may be further included have. Among the extracts, the sessin extract and the propolis extract are ethanol as the extraction solvent, and all of the extracts that do not specify the extraction solvent use purified water as the extraction solvent. In addition, the fermented extract of Hakkuk-sun fermentation and extract extract three hundred seconds will go through a fermentation process for a certain period, respectively.

First, the Nypa fruticans Wurmb is a young shoot of palm trees growing on clean beach tidal flats in Southeast Asia or Australia, including Myanmar, Malaysia, and also called nipa palm. The major constituents of sea bamboo shoots are 28.9 to 45.6% by weight of cellulose, 21.8 to 26.4% by weight of hemicellulose, and the hemicellulose is usually composed of glucuronoxylan. In addition, the content of lignin is also 19.4 ~ 33.8% by weight, which is included in most of the leaves (Pramila Tamunaidu et al., Industrial Crops and Products, 34 [2011] 1423-1428).

In addition, the bamboo shoot contains five times more vitamin E than the human body's daily needs, and contains abundant amounts of saporin, unsaturated fatty acids, vitamin A, beta-carotene, and minerals. It is known. In particular, it has been reported that haejucho vinegar has excellent antibacterial and anti-oxidative effects to prevent gum disease such as periodontitis and gingivitis and to remove bad breath (Nor Adlin Yusoff et al., Asian Pacific journal of Tropical Medicine, Volume. 8, Issue 8, August 2015, Pages 959-605).

In the method for producing a fermented extract of sea cucumber sprout according to the present invention, first, the dried seaweed sprout is chopped to a size of about 5 to 10 mm, and 3 to 5 times the medium temperature purified water is added thereto and mixed well. The mixture thus obtained is fermented at a temperature of 40 to 70 ° C. for about 7 to 8 days, the suspended solids are removed, and the remaining fermentation broth is filtered through a fine filter to obtain the above-mentioned bamboo shoot fermentation extract.

If the bamboo shoots are extracted at a temperature below 40 ° C., the active ingredient may not be effectively extracted or sufficient fermentation may not occur. On the contrary, when the extraction temperature exceeds 70 ° C., the active ingredient may be destroyed or lost, which is not preferable. The sea cucumber sprout extract is enhanced through the fermentation and aging process, the antioxidant power of the polyphenol component is enhanced, the vivid and irritating taste disappears.

Secondly, Asiasari Radix is a root stem of Korean or Korean cephalopods with rat vines, which are spicy enough to numb the tongue but are not toxic. In oriental medicine, it is used for arthritis, headache, neuralgia, antipyretic effect due to analgesic, anti-inflammatory and antioxidant effects, and is known to have a whitening effect by inhibiting tyrosinase activity.

In the method of preparing the sacin ethanol extract, the dried sacin is chopped and the extract is added by reflux cooling extraction at 80 ° C. for 3 hours by adding 5-8 times the amount of 20% ethanol extract solvent in a weight ratio. Subsequently, the extract obtained using a centrifuge was centrifuged at 1,000 rpm for 15 minutes to secure a supernatant, and the supernatant was filtered through Whatman filter paper to obtain a sacinin ethanol extract.

Pine leaf is a leaf of an evergreen conifer tree of the pine family, and its constituents vary slightly depending on the variety and harvesting time, but mainly terpenetine oil, cineole and salinigrin. , Coniferin, P-cymen, densipimaric acid, retenene and chlorophyll, protein, phosphorus, iron, enzymes, minerals, fat-soluble vitamin A and vitamin C. It is contained.

Because of these components, pine needles are known to be effective in regenerating infections or wounds with antimicrobial activity. In particular, pine needles have more than 10 times more phytoncide than other plants, which strongly kills bacteria or fungi in the air, prevents pest invasion, and removes pathogens that are harmful to humans.

The method of obtaining the pine needles hot water extract, washed pine needles collected from Korean pines and cut to 2 ~ 3 Cm length, and then added 7 ~ 10 times purified water by weight ratio and 5 ~ 8 at 95 ~ 100 ℃ After heating for a time, cooled to room temperature. The cooled mixture is then filtered to remove solids and concentrated in vacuo to give a pine needle extract of 55-65 ° Brix.

Purslane (Portulaca oleracea L.) is a year-round weed with purslane and is also known as five-haengcho, jangmyeongchae, machichae, and is native to roadsides and gardens. The stem is about 15 ~ 30cm high, hairless, reddish red, and many branches are scattered obliquely on the ground. The root is white, but scattered by hand turns red like a main stem. The leaves are inverted egg-shaped, rounded at the ends and narrow at the bottom to form short petioles.

The components of purslane contain small but adrenal hormones, noradrenalin and dopamine, as well as large amounts of organic acids (apple, lemon, citric acid) and amino acids (glutamic acid, aspartic acid, alanine). , Cardiac glycosides, and intraquinone glycosides.

In the present invention, the preparation method of purslane hot water extract, 2 to 3 times the amount of purified water to purslane and heated at 100 ℃ for about 30 to 40 minutes, the solids are removed and the remaining filtrate is prepared by filtration. For reference, according to Korean Patent Registration No. 10-0272792 (August 30, 2000), the purslane extract has been reported to have a nitotin removal efficiency of about 89%.

Ulmi Cortex is a bark peeled from the root or bark of the elm tree (Ulmus macrocarpa Hance), which belongs to the elm family, and has been used as a folk medicine for a long time in Korea and Northeast Asia. According to the synonym dictionary, it is said that milk milk is sweet in taste and flat in nature, acting on the parenteral, stomach, menopause, and colonoscopy, urinates well, swells, stool and lowers the heat of the stomach. .

Studies on the constituents of milky skin have so far been carried out on sterols (β-sitosterol, phytosterol, stigmasterol), hydrolyzed tannins, resins, fatty acids, and catechin and catechin complexes, procyanidins. Has been mainly progressed. And it has been reported that catechin polymers, called procyanidin oligomers isolated from milk extract, inhibit periodontal disease by inhibiting bacterial collagenase (J Periodontal Res. 2003 Jun; 38 (3): 282-289).

In the manufacturing method of hot milk extract, milky skin is first crushed into 2 ~ 3Cm size, and then it is put into the extraction tank with 10 ~ 20 times of purified water by weight ratio, and it is 5 ~ 6 hours at extraction temperature between 90 ~ 100 ℃. While extracting. The solids are then removed and the filtrate is filtered to produce a milky skin extract.

Three hundred herb (Samrurus Chinensis Bail) is a perennial herb belonging to the genus of three hundred and three hundred, and is called three hundred seconds because two or three leaves on the upper part of this plant stem are white. The main components of the three hundred vinegar are quercetin, quercitrin, isoquercitrin, and hyperin in the leaves, and rutin and starch in the stem. Contains a lot of essential oils methyl-n-nonyl ketone (Methyl-n-nonyl ketone). Among these components, quercetin, quelcitrin, isocuelcitrin, hyperin, rutin, and the like are flavonoids that have antioxidant activities such as polyphenols, that is, free radical scavenging. In oriental medicine, three hundred seconds has been used for detoxification, rubella, stool, hypertension, diuresis, etc., and is also used for the treatment of customary arthritis, such as underwear or external use.

The method of preparing the fermented extract of three hundred seconds, first put the selected and washed three hundred seconds into the extraction tank, and 5 to 8 times the amount of purified water in a weight ratio to the three hundred seconds, and then extracted the hot water at 95 ~ 100 ℃ 6-8 hours to extract the three hundred seconds extract Get Subsequently, brown sugar and malt were mixed with the concentrated extract to the 300 sec extract and fermented at 35-40 ° C. for about a month. Then, the supernatant was separated and filtered to obtain a 300 sec fermentation concentrate.

Finally, propolis is a fat-soluble complex obtained from honeycomb that is a mixture of bees collected by plant and secreted by the bee's own salivary glands. This propolis is a resinous, tacky, rubbery material mainly used for the purpose of preventing the inflow of bacteria from outside by applying to the honeycomb doorway, or applied to the gap of the honeycomb to block rain and cold.

Propolis contains high amounts of flavonoids and natural organic substances, especially flavonoids, including cinnamic acid and caffeic acid esters, which are used to treat anticancer, antibacterial, antibacterial, antiviral, anti-inflammatory, antifungal, antioxidant and allergic dermatitis. It has been reported to indicate helpful bioactive mechanisms.

In the present invention, the method for producing a propolis ethanol extract first removes foreign matters by screening the propolis lump, and then added to the extraction tank with 95% ethanol of 3 to 4 times by weight ratio, extracting about 20 to 30 days while slowly stirring And aged. Subsequently, primary filtration was performed using a microfilter having a pore size of 3 to 4 μm, and the filtrate was cooled to a temperature of minus 15 to 20 ° C., followed by a dewaxing process for separating fat, wax, and resin components, followed by remaining filtrate. It is filtered once more to obtain the fluoropolis ethanol extract of the present invention.

The herbal extract extract for gargle according to the present invention includes a sea cucumber sprout fermentation extract as an active ingredient, preferably the sessin ethanol extract and pine needle extract, purslane extract, milk baekpi extract, The 300 seconds fermented extract and the propolis ethanol extract each contain 5 to 20 parts by weight, preferably 10 parts by weight.

On the other hand, in order to manufacture a gargle solution that can actually be used in the human body by using the herbal extract for gargle according to the present invention, it is preferable to contain 0.5 to 1.5% by weight of the herbal composition of the present invention in the gargle solution. In addition to the herbal composition of the present invention, the gargle solution contains ethyl alcohol and glycerin, and a small amount of sodium fluoride, allantoin, xylitol, sodium saccharin, malic acid, lactic acid, citric acid, menthol, caramel pigment, artificial flavor, etc. It may include, the remaining amount includes purified water.

[Examples and Comparative Examples]

1) Preparation of herbal extract composition for gargle

Seaweed sprout fermentation extract and sessin ethanol extract, pine needle hot water extract, purslane hot water extract, milk white skin hot water extract, three hundred seconds fermentation extract and propolis ethanol extract using the composition and content ratio (part by weight) as shown in the following Table 1 The herbal extract composition was prepared.

Ingredient Content ratio (unit; parts by weight) Example 1  Example 2  Comparative Example 1 Sea Bamboo Shoot Fermentation Extract 10 10 - Sesine Ethanol Extract - 10 10 Pine needle hot water extract - 10 10 Purslane hydrothermal extract - 10 10 Milky Skin Hot Water Extract - 10 10 Three hundred seconds fermented extract - 10 10 Propolis Ethanol Extract - 10 10

2) Preparation of Gargle Solution

The gargle solution was prepared using the herbal extract compositions of Examples 1,2 and Comparative Example 1. The contents of the herbal extract composition in the gargle solution of Examples 1 and 2 and Comparative Example 1 were all equal to 1.2% by weight, wherein ethyl alcohol and glycerin were 6.0% by weight, respectively, xylitol 0.8% by weight and saccharin sodium 0.25. Wt%, malic acid 0.1 wt%, lactic acid 0.1 wt%, allantoin 0.05 wt%, caramel pigment 0.05 wt%, menthol 0.02 wt%, sodium fluoride 0.02 wt%, citric acid 0.01 wt%, artificial flavor 0.1 wt% and purified water 85.3 wt% The composition was at the ratio of.

In addition, the red ginseng extract-containing gargle solution described in Example 1 (Table 1) of Patent No. 10-1358507 (January 27, 2014) introduced as a background art was used as the gargle solution of Comparative Example 2.

[Test of Inhibition Effect of Oral Microbial Growth]

1) Test method

Snyder test (Snyder test) was performed to examine the growth inhibitory effect on the oral microorganisms of the gargle solutions of Examples 1 and 2 and Comparative Examples 1 and 2, respectively. The Snyder test method is to measure the activity of the lactic acid producing bacteria on the basis of the color of the bacteria contained in the plaque or saliva by colorimetric method, the evaluation criteria of the bacterial activity for each color was calculated as shown in Table 2.

Badge Color Bacterial activity Score blue Inactivity 1 point green Slightly active 3 points yellowish green Moderate activity 5 points yellow Highly active 7 points

2) Evaluation method

A total of 20 subjects were selected from 7 males, 13 females, and randomly classified into 5 groups (G1 to G5) of 4 persons each, followed by Example 1 (G1), Example 2 (G2), and Comparative Example 1 (G3). ), Comparative Example 2 (G4), and a control group (G5) were assigned. The subjects of the G1 to G4 groups were each gargled for 10 minutes with the gargle solutions of Examples 1 and 2 and Comparative Examples 1 and 2 before breakfast each day for 30 days, and then 0.2 ml of each saliva of each subject was tested for the Snyder test. Inoculated in medium (Difco, USA). Subjects in the G5 group were inoculated with saliva in the same manner without gargles.

After culturing the saliva-inoculated medium of each subject for 3 days at 37 ° C., observe the change of color every 24 hours, and calculate the evaluation score for the activity of bacteria according to the criterion of FIG. The average values are listed in Table 3 below.

division  G1 group G2 Group G3 Group G4 group G5 group Score 2.8 2.3 3.8  3.1 5.6

3) Evaluation result

As shown in the results of Table 3, the G1 and G2 group using the gargle solution containing the herbal extract composition of the present invention is compared to the oral cavity G3 and G4 group using the gargle solution of Comparative Examples 1,2 and G5 group of the control group The result showed that the growth activity of the microorganisms was significantly suppressed. In particular, the G1 group using only the bamboo shoot fermentation extract as a herbal extract composition showed a significant effect, but a better effect in the G2 group using the herbal extract composition of Example 2.

[Measurement of satisfaction with the goggle effect]

1) Test method

The following table shows the satisfaction of self-feeling about the improvement of bad breath, the degree of gum bleeding, the taste (irritation) of the goggle solution, and the refreshing feeling after the goggle for 10 times every three days for the G1 ~ G4 group among the subjects. The survey was conducted according to the evaluation criteria as shown in 4.

 Satisfaction Category Score Much better / very good 9 Slightly better / slightly better 7 It is normal 5 No change / slightly bad 3 Rather worse / very bad One

2) Evaluation method and result

The scores according to the evaluation results were calculated for each group, and then the average value was calculated and listed in Table 5 below.

Evaluation item G1 group G2 Group G3 Group G4 group Bad breath removal effect 7.2 7.4 6.3 7.0 Gum bleeding degree 5.5 5.4 5.4 5.6 Gargle's taste 6.8 7.2 7.0 6.7 Cool after goggles 6.8 7.0 6.6 6.8 Sum 26.3 27.0 25.3 26.1

As shown in Table 5, the highest evaluation score was obtained as a whole in the G2 group using the goggle solution of Example 2, G3 using the goggle solution of Comparative Examples 1 and 2 in the G1 group using the goggle solution of Example 2 and The results were more satisfactory than the G4 group.

For reference, the overall low score for gum bleeding was attributed to the fact that most subjects had no symptoms of gum bleeding regardless of the use of gargle solution.

Claims (4)

10 parts by weight of sea cucumber sprout fermentation extract, including 10 parts by weight of sesine ethanol extract, pine needle hydrothermal extract, purslane hydrothermal extract, milky skin hydrothermal extract, triticale fermented extract and propolis ethanol extract, respectively
The sea cucumber sprout fermentation extract is a fermented sea cucumber sprout in 3 to 5 times the purified water by weight ratio to ferment for 7-8 days at a temperature of 40 ~ 70 ℃,
The three hundred seconds fermentation extract, the three hundred seconds in a weight ratio of 5 to 8 times the purified water was extracted by hot water extraction at 95 ~ 100 ℃ 6-8 hours, and then mixed with brown sugar and malt in the obtained three hundred seconds extract at a month at 35 ~ 40 ℃ Herbal extract composition for gargle, characterized in that the fermentation during. delete delete delete