KR101622230B1 - Liquid compositions for preventing gingivitis and periodontitis containing natural herbal extracts - Google Patents

Abstract

The present invention relates to a composition for preventing gingivitis and periodontitis comprising ginseng extract, horse rinse extract, sesquin extract, spectral extract, acanthopanax extract, Saururus chinensis extract, and the like. In addition to Streptococcus mutans, A method for preventing periodontal disease, prevention of periodontal disease and gingivitis, prevention of periodontal disease by inhibiting the formation of plaque having a strong sterilizing effect on Porphyromonas gingivalis, / RTI >

Description

FIELD OF THE INVENTION [0001] The present invention relates to a gingivitis, a periodontal disease prevention composition, and a method for producing the same, which contains a natural herbal medicine extract having a sterilizing effect against a mite bacteria and a periodontal disease causative microorganism,

More particularly, the present invention relates to a composition for preventing gingivitis and periodontal disease comprising red ginseng extract, horse riding extract, sesquin extract, spectral extract, acanthopanax extract, Saururus chinensis extract and Streptococcus mutans (Porphyromonas gingivalis) as well as Streptococcus mutants, as well as Gram-negative bacteria, such as Porphyromonas gingivalis, thereby preventing the formation of plaque and preventing the periodontal disease and gingivitis, Gingivitis, a composition for preventing periodontal disease, and a method for producing the same.

Korean Patent Publication No. 1993-0000059 discloses an oral composition for preventing tooth decay and is characterized by containing 0.01 to 3% by weight of one or more kinds of plant extracts such as horse riding, An oral cavity composition for preventing tooth decay is disclosed.

Korean Patent No. 10-0886494 discloses a methionine inhibitor, an oral composition and a food containing the same, and is characterized by having at least one kind of methioninase inhibitory effect selected from the group consisting of crows and the like A composition for oral cavity suppressing bad breath characterized by containing a plant extract as an effective ingredient.

Korean Patent No. 10-1157088 discloses a bad breath improving oral composition based on a tangerine and a method for producing the same, wherein a ratio of tangerine to acid is 100: 1, and the mixture is mixed with a mixture of tangerine and sancho, and the mixture is heated and cooled to form an extract of tangerine including the extracted gentian sachet. The oral care composition for oral cavity improvement based on tangerine is disclosed.

Korean Patent Laid-Open Publication No. 10-2012-0093607 relates to a liquid composition for oral use, which is a composition for oral cavity, which is capable of inhibiting bacteria in the oral cavity and containing a dentifrice preventive agent, antimicrobial agent, sweetener, flavor, preservative, pH adjusting agent, Wherein the plant extract comprises at least one selected from the group consisting of green tea extract, saururus chinensis extract, saururus chinensis extract, and cinnabar extract, in an aqueous liquid composition containing a vegetable extract and purified water.

Korean Patent Laid-Open No. 10-2011-0088978 discloses an oral composition containing an extract of a herbal medicine component, which comprises an extract of sesame, clove and peony.

According to the embodiments of the present invention, it is an object of the present invention to provide a gingivitis and periodontal disease prevention composition which is excellent in tooth decay prevention, prevention of gingivitis and periodontitis as well as malodor removing function.

In addition, the present invention relates to an antimicrobial effect against tooth decubitus bacteria, in particular, an antimicrobial effect against Streptococcus mutans typified by a Toxicum gum, and is useful as a Gram-negative bacterial strain, Porphyromonas gingivalis The present invention also provides a composition for preventing periodontal disease.

The gingivitis and periodontal disease prevention composition containing the extract of natural herbal medicine component according to the embodiments of the present invention can be used for the prevention of gingivitis and periodontal disease including extracts of ginseng extract, Wherein the herbal composition extract comprises from 0.0001% by weight to 10% by weight of the total composition of the composition, and the other ingredients including the allantoin, the sweetener, the wetting agent, the solubilizer, the preservative, the viscosity adjusting agent, the flavor, the colorant, , Which contains Gram positive bacillus Streptococcus mutants and is resistant to Gram negative bacillus, Porphyromonas gingivalis, which is a Gram positive bacillus, It has germicidal effect, inhibits plaque formation, and has a function of preventing tooth decay, preventing periodontitis, preventing and treating gingivitis.

According to one aspect, the herbal composition extract may comprise 0.001 wt% to 5 wt% of the total composition.

According to one aspect, the fluorine compound includes at least one material selected from the group consisting of sodium fluoride, sodium fluorophosphate, and tin fluoride, and the content of the fluorine compound may be 0.001 wt% to 2.0 wt% have.

According to one aspect, the content of the allantoin may be comprised between 0.01 wt% and 2.0 wt%.

According to one aspect of the present invention, the sweetener comprises one or more selected from the group consisting of eucalyptol, sodium saccharin, xylitol, erythritol, stevia, and aspartame, and the content of the sweetener is 0.001 wt% to 10 wt% ≪ / RTI >

According to one aspect of the present invention, the wetting agent comprises one or more compounds selected from the group consisting of glycerin, propylene glycol, polyethylene glycol and sorbitol, and the content of the wetting agent is 1.0 wt% to 10 wt% .

According to one aspect, the solubilizing agent comprises at least one selected from the group consisting of propylene glycol, polyoxyl 40hydrogenated castor oil, poloxamer, and polyethylene glycol, and the solubilizing agent content is 0.01 By weight to 10% by weight.

According to one aspect, the preservative may include one or more selected from the group consisting of methylparaben, propylparaben, sodium benzoate, and the preservative may be contained in an amount of 0.001 wt% to 1.0 wt%.

According to one aspect, the flavoring agent comprises a mixture of one or more selected from the group consisting of L-menthol, apple mint oil, peppermint oil, lemon oil and spearmint oil, and the content of the flavoring agent is 0.0001 By weight to 0.1% by weight.

According to one aspect of the present invention, the coloring agent includes one or a mixture of two or more selected from the group including caramel colorant, copper chlorophyllin sodium, iron chlorophyllin sodium, cacao color, cicada blue color, safflower yellow color, red cabbage colorant , And the content of the colorant may be 0.0001 wt% to 0.01 wt%.

According to one aspect of the present invention, the pH adjusting agent comprises a mixture of one or more selected from the group consisting of citric acid, sodium citrate, phosphoric acid, tartaric acid, malic acid, lactic acid, sodium tartrate, To 1.0% by weight.

Meanwhile, the method for preparing gingivitis and periodontal disease prevention composition containing the extract of natural herbal medicine component according to the above-described embodiments of the present invention is characterized in that the gingivitis and periodontal disease prevention composition containing ginseng extract and ginseng extract, comprising horse extract, horse radish extract, Preparing a further component including a fluorine compound, an allantoin, a sweetener, a wetting agent, a solubilizing agent, a preservative, a viscosity controlling agent, a flavoring agent, a coloring agent and a pH adjusting agent; Mixing the purified water with the purified water so that the total weight is 100% by weight, and the gypsum salt and the periodontitis prevention composition are prepared. The extract of the herbal medicine component is contained in an amount of 0.0001 to 10% Wherein the composition for preventing gingivitis and periodontitis is composed of Gram positive bacillus instinct It has a germicidal effect against bacteria including Porphyromonas gingivalis including Gram negative bacillus, including Streptococcus mutants, and inhibits plaque formation, Periodontitis, gingivitis prevention and treatment effect.

According to one aspect of the present invention, the step of extracting the herbal medicine component extract comprises: washing 1 kg of the outposts to be the target of the herbal medicine ingredient extract; 5.0 kg of purified water and 5.0 kg of butylene glycol Extracting the purified water and the outpost to which the butylene glycol has been administered at 30 ° C for 24 hours and filtering to obtain an extract, and filtering the extract with 0.2 μm bacterium.

As described above, according to the embodiments of the present invention, since it contains red ginseng extract, horse riding extract, sesquin extract, spectral extract, acidulant extract and saururus chinensis extract, Thereby inhibiting the induction of anti-inflammatory substances and preventing and treating periodontal disease simultaneously.

FIG. 1 is a block diagram showing the construction and manufacturing method of a gingivitis and periodontal disease prevention composition according to an embodiment of the present invention.
FIG. 2 is a block diagram illustrating a process of extracting a herbal composition extract of a gingivitis and periodontal disease prevention composition according to an embodiment of the present invention.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, embodiments will be described in detail with reference to the accompanying drawings, but the present invention is not limited to or limited by the embodiments. In describing the embodiments, a detailed description of well-known functions or constructions may be omitted so as to clarify the gist of the present invention.

Hereinafter, the present invention will be described in detail with reference to Figs. 1 and 2 and Examples and Experimental Examples described later. However, these embodiments are only for the understanding of the present invention, and the scope of the present invention is not limited to these embodiments. Unless otherwise specified in the following Experimental Examples and Examples, '%' refers to '% by weight'.

The composition for preventing gingivitis and periodontitis according to this embodiment is composed of a natural herbal medicine extract such as red ginseng extract, horse riding extract, sesquin extract, spectral extract, sancho extract, saururus chinensis extract and the like, and has no oral mucous membrane irritation and side effects, It is possible to simultaneously prevent and treat tooth decay and periodontitis, and also to suppress and remove bad breath in the oral cavity.

According to this embodiment, the natural herbal medicine component extract as an active ingredient may contain 0.0001% by weight to 10% by weight, preferably 0.001% by weight to 5% by weight, of the total weight of the composition for preventing gingivitis and periodontitis. Herbal extracts may include red ginseng extract, horse riding extract, sesquin extract, spectral extract, sancho extract, and saururus chinensis extract. On the other hand, each of the herbal medicine extracts is washed thoroughly with 1 kg of the excreta, 5.0 kg of purified water and 5.0 kg of butylene glycol are added, and the mixture is extracted at 30 ° C for 24 hours and then filtered to obtain about 9.0 kg of the extract. This was prepared by 0.2 쨉 m bacterial filtration.

When the content of the herbal medicine component extract is less than 0.0001% by weight, the efficacy is weak. When the content of the herbal medicine component extract is more than 10% by weight, the feeling of use is poor and cytotoxicity is likely to occur. .

In addition, the composition for preventing gingivitis and periodontitis may be a component other than the herbal composition extract (hereinafter referred to as "other component") in an appropriate amount depending on the kind of gingivitis, the kind of the periodontitis prevention composition and the purpose of use. For example, the composition for preventing gingivitis and periodontitis may be an oral cleanser (goggle). In this case, the composition for preventing gingivitis and periodontitis may further contain, as other components, a fluorine compound, an allantoin, a sweetener, a wetting agent, a solubilizing agent, Flavoring agents, coloring agents, pH adjusting agents, and the like.

More specifically, the fluorine compound acts to prevent tooth decay by increasing the acid resistance of teeth and can use at least one of sodium fluoride, sodium fluorophosphate, and tin fluoride. The content of the fluoride compound is 0.001 wt% to 2.0 wt% desirable.

Allantoin can be used by preventing irritation and tooth whitening, and its content is preferably 0.01 wt% to 2.0 wt%.

As the sweetening agent, eucalyptol sodium, saccharin sodium, xylitol, erythritol, stevia, aspartame, etc. may be used alone or in admixture of two or more, preferably 0.001 to 10% by weight.

As the wetting agent, glycerin, propylene glycol, polyethylene glycol, sorbitol and the like may be used alone or in admixture of two or more, preferably 1.0 wt% to 10 wt%.

The solubilizing agent may be one or a mixture of two or more of propylene glycol, polyoxyl 40 hydrogenated castor oil, poloxamer, and polyethylene glycol, preferably 0.01 wt% to 10 wt%.

The preservative may be selected from the group consisting of methylparaben, propylparaben, sodium benzoate, and the like, preferably 0.001 wt% to 1.0 wt%.

The flavoring agent may be one or a mixture of two or more kinds of L-menthol, apple mint oil, peppermint oil, lemon oil, spearmint oil and the like, preferably 0.0001 wt% to 0.1 wt%.

The colorant may be one or a mixture of two or more of caramel colorant, copper chlorophyllin sodium, iron chlorophyllin sodium, cacao colorant, ginger blue color, safflower yellow color, red cabbage colorant, .

The pH adjusting agent may be one or a mixture of two or more of citric acid, sodium citrate, phosphoric acid, tartaric acid, malic acid, lactic acid, sodium tartrate, etc., preferably 0.001 wt% to 1.0 wt%.

Example

The gingivitis and periodontitis prevention compositions of Examples 1 to 4 and Comparative Examples 1 to 3 were prepared by mixing herbal ingredients extract and other ingredients, and the components and contents of Examples 1 to 4 and Comparative Examples 1 to 3 were as shown in the following table 1. Herein, the gingivitis and periodontitis prevention composition according to Examples 1 to 4 contains different contents of herbal medicine extracts, and in the case of gingivitis and periodontitis prevention compositions according to Comparative Examples 1 to 3, . Each of the herbal composition extracts was used as it was.

Furtherance Example 1 Example 2 Example 3 Example 4 Comparative Example 1 Comparative Example 2 Comparative Example 3 (%) (%) (%) (%) (%) (%) (%) Red ginseng extract 0.005 0.01 0.1 0.5 0.1 - - Horse riding extract 0.01 0.05 0.1 0.25 0.25 - - Sesin extract 0.001 0.01 0.01 0.1 - 0.1 - Spectral extract 0.01 0.03 0.05 0.1 - 0.1 - Sancho extract 0.05 0.1 0.15 0.2 - - 0.2 Saururus chinensis extract 0.25 0.3 0.5 0.8 - - 0.8 Sodium fluoride 0.02 0.02 0.02 0.01 0.01 0.02 0.02 Allantoin 0.05 0.05 0.05 0.05 0.05 0.05 0.05 glycerin 6.00 6.00 6.0 6.0 6.0 6.0 6.0 RH40 * 0.1 0.1 0.2 0.2 0.2 0.2 0.2 Xylitol 0.8 0.8 0.8 0.8 0.8 0.8 0.8 Sodium saccharin 0.015 0.015 0.015 0.015 0.015 0.15 0.015 Lactic acid 0.1 0.1 0.2 0.2 0.2 0.2 0.2 Malian 0.1 0.3 0.3 0.1 0.3 0.3 0.3 Citric acid 0.1 0.2 0.2 0.1 0.2 0.2 0.2 Sodium citrate 0.05 0.1 0.2 0.1 0.2 0.2 0.2 Sodium benzoate 0.1 0.1 0.1 0.1 0.1 0.1 0.1 L-menthol 0.07 0.07 0.1 0.1 0,1 0.1 0.1 Pigment 0.06 0.06 0.06 0.06 0.06 0.06 0.06 Spices 0.05 0.05 0.05 0.05 0.05 0.05 0.05 ethanol 10.0 8.0 10.0 8.0 8.0 8.0 8.0 Purified water to 100 to 100 to 100 to 100 to 100 to 100 to 100

In Table 1, RH40 represents Polyoxyl 40 Hydrogenated Castor Oil.

Experimental Example 1

In Experimental Example 1, the antimicrobial activity test for Gram positive bacillus against the gingivitis and periodontitis prevention composition according to Examples 1 to 4 and Comparative Examples 1 to 3 was carried out, and the results are shown in Table 2 .

The bactericidal effect of the sample solutions described in Examples 1 to 4 and Comparative Examples 1 to 3 was confirmed by using Streptococcus mutans, Gram-positive bacteria. The strain used for the test was ATCC 27351 and the medium was Brain heart infusion (DIFCO, USA), Tryptic soy agar (DIFCO, USA), D / E Nutraging broth (D / E Neutralizing broth (DIFCO, USA)) and physiological saline. The strain was inoculated into Brain heart infusion and cultured at 37 ± 1 ° C. for 24 hours. The culture was diluted to adjust the initial bacterial count to (1 to 9) × 10 ⁵ CFU / ml It was used for the test. The test strain was inoculated in 20 ml of the stock solution and allowed to stand at room temperature for 30 seconds and 1 minute, and the viable cell count was measured to confirm the reduction rate of sterilization relative to the initial number of bacteria. The number of bacteria was measured after inoculation of the test strain with sterile physiological saline, and the number of bacteria was indicated as the initial number of bacteria. The initial dilution stage of all experiments was neutralized using D / E Neutralizing broth. When the bacteria were grown in the medium, the number of bacteria on the medium was multiplied by the dilution factor And the number of bacteria was calculated. In the case of no growth of bacteria in the medium, it was multiplied by the dilution factor in the neutralization step and expressed as "less than 10 (<10)". Bacterial counts were performed at all stages using a Tryptic soy agar. The number of live cells was calculated by N = C × D, where N is the number of viable cells, C is the number of colonies and D is the dilution factor. ] × 100 (where R: bacterial reduction rate, A: initial number of bacteria, B: number of bacteria after a certain time).

division The initial [CFU / ml] After 30 seconds [%] After 1 minute [%] Example 1 8.6 × 10 ⁵ &Lt; 10 (99.9% or more) &Lt; 10 (99.9% or more) Example 2 8.6 × 10 ⁵ &Lt; 10 (99.9% or more) &Lt; 10 (99.9% or more) Example 3 8.6 × 10 ⁵ &Lt; 10 (99.9% or more) &Lt; 10 (99.9% or more) Example 4 8.6 × 10 ⁵ &Lt; 10 (99.9% or more) &Lt; 10 (99.9% or more) Comparative Example 1 8.6 × 10 ⁵ 1.2 x 10 ³ (99.8%) 3.4 x 10 ² (99.9%) Comparative Example 2 8.6 × 10 ⁵ 2.5 x 10 ³ (99.7%) 1.5 x 10 ² (99.9%) Comparative Example 3 8.6 × 10 ⁵ 2.3 x 10 ³ (99.7%) 5.4 × 10 ² (99.9%)

According to Experimental Examples 1 and 2, it can be seen that in Examples 1 to 4, bacteria were killed to less than 10 both after 30 seconds and after 1 minute. In Comparative Examples 1 to 3, it was found that the germicidal power against bacteria was insufficient at 30 seconds and 1 minute after the administration of the germicidal composition of the present invention. Thus, the compositions for preventing gingivitis and periodontitis according to Examples 1 to 4 exhibited a strong sterilizing power against Gram- .

Experimental Example 2

In Experimental Example 2, germicidal and gingivitis-preventing compositions according to Examples 1 to 4 and Comparative Examples 1 to 3 were tested for bactericidal effect against Gram negative bacillus, and the results are shown in Table 3.

The germicidal activity against the gram-negative bacteria, Porphyromonas gingivalis, of the samples shown in Examples 1 to 4 and Comparative Examples 1 to 3 was tested.

The strain used for the test was KCTC 5500, and the medium was inoculated at a concentration of 5 × 10 ⁴ cfu / mL in BBL Colombia liquid agar medium and BBL Colombia blood agar medium containing 5% of hematopoietic red cells at 72 (± 1) ° C for 72 Incubated in an anaerobic incubator for a period of time. After culturing, the culture was diluted to adjust the initial number of bacteria (1 to 9) to 104 CFU / ml and used for the test. The test strain was inoculated in 20 ml of the stock solution and allowed to stand at room temperature for 30 seconds and 1 minute, and the viable cell count was measured to confirm the reduction rate of sterilization relative to the initial number of bacteria. The number of bacteria was measured after inoculation of the test strain with sterile physiological saline, and the number of bacteria was indicated as the initial number of bacteria. The initial dilution stage of all experiments was neutralized using D / E Neutralizing broth. When the bacteria were grown in the medium, the number of bacteria on the medium was multiplied by the dilution factor And the number of bacteria was calculated. In the case of no growth of bacteria in the medium, it was multiplied by the dilution factor in the neutralization step and expressed as "less than 10 (<10)". The number of live cells was calculated by N = C × D, where N is the number of viable cells, C is the number of colonies and D is the dilution factor. × 100 (where R: bacterial reduction rate, A: initial number of bacteria, and B: number of bacteria after a certain time).

division The initial [CFU / ml] After 30 seconds [%] After 1 minute [%] Example 1 5.6 x 10 ⁴ &Lt; 10 (99.9% or more) &Lt; 10 (99.9% or more) Example 2 5.6 x 10 ⁴ &Lt; 10 (99.9% or more) &Lt; 10 (99.9% or more) Example 3 5.6 x 10 ⁴ &Lt; 10 (99.9% or more) &Lt; 10 (99.9% or more) Example 4 5.6 x 10 ⁴ &Lt; 10 (99.9% or more) &Lt; 10 (99.9% or more) Comparative Example 1 5.6 x 10 ⁴ 2.2 x 10 ⁴ (60.7%) 3.6 x 10 ³ (93.6%) Comparative Example 2 5.6 x 10 ⁴ 1.5 x 10 ⁴ (73.2%) 2.7 x 10 ³ (95.1%) Comparative Example 3 5.6 x 10 ⁴ 1.3 x 10 ⁴ (76.7%) 3.6 x 10 ³ (93.6%)

According to Experimental Examples 1 and 2, it can be confirmed that in Examples 1 to 4, bacteria were killed to less than 10 at 30 seconds and 1 minute after. In Comparative Examples 1 to 3, it was confirmed that the sterilizing power against the bacteria was insufficient at 30 seconds and 1 minute after. Therefore, it can be seen that the gingivitis and periodontitis prevention compositions according to Examples 1 to 4 have germicidal power against Gram-negative bacteria.

Experimental Example 3

In Experimental Example 3, plaque formation inhibition tests were carried out on the gingivitis and periodontal disease prevention compositions according to Examples 1 to 4 and Comparative Examples 1 to 3, and the results are shown in Table 4.

The plaque formation inhibition test was conducted by using a double blind method in which subjects were controlled not to be able to distinguish the samples. Before the test, all of the examinees examined the baseline of the flag index, and after scaling, underwent a two week residual effect elimination period. For the test, 10 drops of tooth coloring solution were mixed with 20 ml of water, and the mixture was spit out for 10 minutes in the mouth. The tooth was lightly soaked with water and checked for color. The rating criteria of the dental floss index were determined by applying "Turesky modification" Respectively. The test solution was used 3 times a day for 2 months, and oral test was performed once a month to confirm the inhibition of plaque formation.

Sample Initial value After 1 month Two months later Improvement index Example 1 1.52 + - 0.23 1.23 + - 0.21 0.92 + 0.26 0.60 0.21 Example 2 1.50 0.25 1.19 0.31 0.89 + - 0.34 0.61 + 0.19 Example 3 1.49 0.34 1.14 ± 0.24 0.81 0.21 0.68 ± 0.23 Example 4 1.55 + 0.29 1.12 ± 0.23 0.77 + - 0.24 0.78 + - 0.24 Comparative Example 1 1.52 + - 0.13 1.34 ± 0.23 1.21 0.27 0.31 + 0.18 Comparative Example 2 1.51 + - 0.22 1.27 ± 0.26 1.20 ± 0.19 0.31 + 0.24 Comparative Example 3 1.53 + - 0.26 1.35 ± 0.27 1.25 0.22 0.28 ± 0.19

For reference, in Experimental Example 3, the plaque formation score criterion is as follows.

0: no plaque generated at all

1: Plaque is formed in the gingival margin

2: When a plaque of 1 mm or less is formed as a ring on the cervical portion

3: When a plaque of 1 mm or more is formed in a ring shape on a tooth surface less than 1/3 of the cervical portion side

4: When a plaque is formed on a tooth surface less than 2/3 of the cervical portion side

5: A plaque is formed on a tooth surface of 2/3 or more on the cervical portion side

As can be seen from Experimental Example 3 and Table 4, it can be seen that in Examples 1 to 4, plaque formation was suppressed and improved after 1 month and 2 months, respectively, And the improvement index is high. Therefore, it can be seen that the gingivitis and periodontitis prevention composition according to Examples 1 to 4 have an effect on suppressing the formation of plaque.

Experimental Example 4

In Experimental Example 4, the gingivitis-inhibitory effect test on the gingivitis and periodontitis prevention composition according to Examples 1 to 4 and Comparative Examples 1 to 3 was carried out, and the results are shown in Table 5.

The gingivitis suppressive effect test was carried out by using a double blind method in which subjects were controlled not to be able to distinguish the samples. Before the test, all of the examinees examined the baseline of the flag index, and after scaling, underwent a two week residual effect elimination period. The gingival per tooth is calculated as the mean value of the indices calculated after dividing the face into the lingual surface and the lingual surface. Each gingivitis was graded from 0 to 3 according to the criteria of "Loe & Silness", and the gingivitis index of teeth was calculated and the gingival index was calculated as the average grade. The test solution was used 3 times a day for 2 months. The oral test was performed once a month to confirm the degree of inhibition of gingivitis.

Sample Initial value After 1 month Two months later Improvement index Example 1 1.59 ± 0.22 1.23 + - 0.22 0.80 + - 0.23 0.99 ± 0.20 Example 2 1.55 + - 0.32 1.21 ± 0.23 0.75 + 0.24 0.80 ± 0.19 Example 3 1.58 + - 0.36 1.16 ± 0.27 0.71 ± 0.19 0.87 + 0.18 Example 4 1.53 + - 0.25 1.17 ± 0.22 0.69 ± 0.18 0.84 0.21 Comparative Example 1 1.59 ± 0.31 1.34 ± 0.28 1.19 ± 0.28 0.40 + 0.17 Comparative Example 2 1.53 + - 0.27 1.37 ± 0.25 1.25 0.29 0.28 0.22 Comparative Example 3 1.57 ± 0.23 1.35 ± 0.22 1.28 ± 0.23 0.29 + 0.15

For reference, the criteria of gingival index in Experimental Example 4 are as follows.

0: Normal value

1: slight inflammation and color change, mild swelling

2: Severe inflammation, redness, swelling, change in color tone of gingiva, and bleeding due to mild stimulation by periodontal probe

3: severe inflammation, redness and swelling, ulceration and natural bleeding

Gingivitis index = overall score / score

As shown in Experimental Example 4 and Table 5, in Examples 1 to 4, gingivitis was suppressed after one month and two months after the initial stage, and the improvement index was higher than that of Comparative Examples 1 to 3 Able to know. Thus, it can be seen that the gingivitis and periodontitis prevention compositions according to Examples 1 to 4 have an effect on inhibiting gingivitis.

As described above, the gingivitis and periodontal disease prevention composition containing the herbal composition extract according to the present invention can be used not only for Gram-positive Streptococcus mutans, but also for Gram-negative bacteria such as Porphyromonas gingivalis, , It is possible to prevent plaque formation, prevent tooth decay, prevent periodontal disease and gingivitis, and exhibit excellent therapeutic effect.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments. For example, it is to be understood that the techniques described may be performed in a different order than the described methods, and / or that components of the described systems, structures, devices, circuits, Lt; / RTI &gt; or equivalents, even if it is replaced or replaced.

Therefore, other implementations, other embodiments, and equivalents to the claims are also within the scope of the following claims.

Claims (13)

Herbal extracts including red ginseng extract, horse riding extract, sesquin extract, spectral extract, acidulant extract, saururus chinensis extract;
Other components including fluorine compounds, allantoin, sweeteners, wetting agents, solubilizers, preservatives, viscosity modifiers, flavoring agents, coloring agents, pH adjusting agents; And
Comprising purified water,
The herbal composition extract is composed of 0.0001% by weight to 10% by weight of the total composition,
The fluorine compound includes at least one substance selected from the group consisting of sodium fluoride, sodium fluorophosphate, and tin fluoride,
Has a germicidal effect against bacteria including Gram positive bacillus Streptococcus mutans and Gram negative bacillus Porphyromonas gingivalis, and has a germicidal effect against bacteria including Gram positive bacillus Streptococcus mutans, including Gram negative bacillus Porphyromonas gingivalis A composition for preventing gingivitis and periodontitis, which contains a natural herbal medicine component extract having a bactericidal effect against a mucus germicidal bacteria and a periodontal disease causative microorganism which inhibits plaque formation.
The method according to claim 1,
The herbal composition extract comprises gentle gums comprising a natural herbal medicine extract having a sterilizing effect on mucus membranaceous germs and gingivitis caused by periodontal disease, the gingivitis composition comprising 0.001% by weight to 5%
The method according to claim 1,
The gingivitis and periodontitis prevention composition containing a natural herbal medicine component extract having a sterilizing effect on a mutant microorganism which is a tooth decay and a periodontal disease causative microorganism which is composed of 0.001 wt% to 2.0 wt% of the fluorine compound.
The method according to claim 1,
A composition for preventing gingivitis and periodontitis, which comprises a natural herbal medicine ingredient extract having a sterilizing effect on a mutant bacterium, which is composed of 0.01% to 2.0% by weight of allantoin, and a zingovirus, which is a cause of periodontal disease.
The method according to claim 1,
Wherein the sweetener comprises a mixture of one or more selected from the group consisting of eucalyptol, saccharin sodium, xylitol, erythritol, stevia, and aspartame,
Wherein the content of the sweetener is 0.001 wt.% To 10 wt.%. The composition for preventing gingivitis and periodontitis contains a natural herbal medicine extract having a sterilizing effect on mutans bacteria and jinji vally bacteria which are causative bacteria of periodontal disease.
The method according to claim 1,
Wherein the humectant includes one or a mixture of two or more selected from the group consisting of glycerin, propylene glycol, polyethylene glycol, sorbitol solution,
A composition for preventing gingivitis and periodontitis, which comprises a natural herbal medicine extract having a sterilizing effect on a mucilage germ and a periodontal disease causative germ, which are causative bacteria of periodontal disease, wherein the content of the wetting agent is from 1.0 wt% to 10 wt%.
The method according to claim 1,
The solubilizing agent includes a mixture of one or more selected from the group consisting of propylene glycol, polyoxyl 40 hydrogenated castor oil, poloxamer, and polyethylene glycol,
The composition for preventing gingivitis and periodontitis according to claim 1, wherein the solubilizing agent comprises 0.01% to 10% by weight of a natural herbal medicine extract having a sterilizing effect on mutans bacteria and periodontal disease causative bacteria of periodontal disease.
The method according to claim 1,
Wherein the preservative comprises a mixture of one or more selected from the group consisting of methylparaben, propylparaben, sodium benzoate,
Wherein the preservative is 0.001 wt.% To 1.0 wt.%. The composition of claim 1, wherein the preservative is 0.001 wt.% To 1.0 wt.% Of a gentle germ extract.
The method according to claim 1,
The flavoring agent includes a mixture of one or more selected from the group consisting of L-menthol, apple mint oil, peppermint oil, lemon oil and spearmint oil,
Wherein the content of the flavoring agent is 0.0001 wt.% To 0.1 wt.%. The composition for preventing gingivitis and periodontitis according to claim 1, wherein the composition contains gentle ginseng extract and ginseng extract.
The method according to claim 1,
Wherein the colorant comprises one or a mixture of two or more selected from the group consisting of caramel colorant, copper chlorophyllin sodium, iron chlorophyllin sodium, cacao color, cicada blue color, safflower yellow color, red cabbage color,
Wherein the content of the coloring agent is 0.0001 wt% to 0.01 wt%. The composition for preventing gingivitis and periodontitis, which contains a natural herbal medicine extract having a sterilizing effect on mutans bacteria and periodontal disease causative bacteria which are causative bacteria of periodontal disease.
The method according to claim 1,
Wherein the pH adjusting agent comprises a mixture of one or more selected from the group consisting of citric acid, sodium citrate, phosphoric acid, tartaric acid, malic acid, lactic acid, sodium tartrate,
A composition for preventing gingivitis and periodontal disease, which comprises a natural herbal medicine extract having a sterilizing effect on a mutant microorganism which is a tooth decay and a periodontal disease causative microorganism which is a causative organism of periodontal disease, wherein the content of the pH adjusting agent is 0.001 wt% to 1.0 wt%.
Extracting a herbal medicine ingredient extract containing red ginseng extract, horse riding extract, sesquin extract, spectral extract, acidulant extract, and Saururus chinensis extract;
Preparing other components including a fluorine compound, an allantoin, a sweetener, a wetting agent, a solubilizing agent, a preservative, a viscosity controlling agent, a flavoring agent, a colorant, and a pH adjusting agent; And
Preparing a composition for preventing gingivitis and gingivitis by mixing the herbal composition extract with the other ingredients and mixing purified water so that the total weight is 100% by weight;
And,
The herbal composition extract comprises 0.0001% by weight to 10% by weight of the total composition of the gingivitis and periodontitis preventing composition,
The composition for preventing gingivitis and periodontitis includes Streptococcus mutants which are Gram positive bacillus in the mouth and a composition containing Gram negative bacillus Porphyromonas gingivalis A method for preparing a composition for preventing gingivitis and periodontitis, which comprises a natural herbal medicine extract having sterilizing effect on bacteria and inhibiting plaque formation and having a bactericidal effect against mutans bacteria,
13. The method of claim 12,
The step of extracting the herbal medicine component extract may comprise:
Washing each outpost of the herbal medicine component extract by 1 kg;
Administering 5.0 kg of purified water and 5.0 kg of butylene glycol to each of the washed outlets;
Extracting the outpoured to which the purified water and the butylene glycol are administered at 30 ° C for 24 hours and filtering to obtain an extract; And
Filtering the extract with 0.2 [mu] m bacterium;
And a method for preparing a composition for preventing periodontal disease, which comprises extracting a natural herbal medicine component having a sterilizing effect on a mushroom bacterium which is a pathogenic bacterium of periodontal disease.

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