KR101354923B1 - Oral hygiene composition using an extract of curcuma longa and an extract eriobotrya japonica - Google Patents

Abstract

The present invention relates to an oral hygiene improvement composition with an excellent periodontal disease treatment effect and an excellent bad breath removal effect, more specifically to an oral hygiene improvement composition containing extracts of Curcuma longa and/or Eriobotrya japonica as an effective component.

Description

Oral hygiene composition using an extract of curcuma longa and an extract Eriobotrya japonica}

The present invention relates to an oral hygiene-promoting composition excellent in improving periodontal disease and removing bad breath. More specifically, Curcuma longa ) extract and / or loquat ( Eriobotrya) japonica ) relates to an oral hygiene-promoting composition comprising the extract as an active ingredient.

Periodontal disease (Periodontal disease) is divided into gingivitis and periodontitis (periodontitis) according to the extent of the disease, a relatively light, fast-recovering form of periodontal disease is called gingivitis, with lesions limited to soft tissues such as gums. If the gums and gums around the progression is called periodontitis. Such periodontal disease progresses as a phenomenon in which the protein present in the oral saliva forms a thin film on the surface of the tooth, and the bacteria adhere to food, exfoliated epidermal cells, white blood cells, and the like. When bacteria are deposited with minerals, they form plaques and produce toxins such as lipopolysaccharide (LPS), nuclease, leukotoxin, and hydrogen sulfide, which cause inflammatory reactions in surrounding tissues, forming periodontal sacs between teeth and gums. Various types of cytokines are secreted from host immune cells and matrix metalloproteinases (MMPs) are secreted by host immune cells and bacteria. As inflammatory reactions intensify, the depth of periodontal sac becomes deeper and causes gum degeneration and bone loss. Periodontal disease progresses (Page and Schroeder, Lab Invest. 1976 Mar 34 (3): 235-49 .; Lourbakos et. al ., Infect Immun. 2001 August; 69 (8): 5121-5130 .; Zhou and Windsor, J Periodontal Res. 2006 Feb 41 (1): 47-54.). Of the more than 350 species of oral microorganisms, less than 5% of bacteria are involved in the development of these periodontal diseases, most of them being Gram-negative anaerobes.

Because the oral environment has less contact with oxygen and is inactive during night hours, dead epithelial cells in the oral cavity, saliva proteins, food wastes, etc., provide a suitable environment for the growth of anaerobic proteolytic bacteria. can do. These bacteria are not only involved in periodontal disease but also in bad breath. They are odors at low concentrations of hydrogen sulfide (SH2) and methyl mercaptan (CH3SH), which are volatile sulfur compounds (VSC) produced by the metabolism of bacteria. (Miyazaki et al ., J Periodontol. 1995 Aug 66 (8): 679-84 .; Krespi et al ., Otolaryngol Head Neck Surg. 2006 Nov 135 (5): 671-6 .; Lee et al ., J Periodontol. 2003 Jan 74 (1): 32-7.).

The main anaerobic bacteria causing the periodontal disease and bad breath, Porphyromonas jinjibaris ( Porphyromonas gingivalis ), Actinobacillus actinomycetemcomitans ), Treponema denticola , Prevotella intermedia ), et al . (Cortelli et al ., Braz Oral Res. 2008 22 Suppl 1: 44-54 .; Paster et al . , Periodontol 2000. 2006 42: 80-7.).

Oral products containing various fungicides such as cetylpyridinium chloride, chlorohexidine hydrochloride, and phenolic compounds are known to sterilize harmful bacteria in the oral cavity, but they are toxic in the oral cavity, discolor the teeth, Therefore, there is a problem in the actual use, such as less active. In recent years, efforts to improve periodontal disease and remove bad breath using herbal extracts such as myrrh, baekbaekpi, horse riding, green tea, and licorice have been actively performed in Korea, but no effective solution has been developed.

An object of the present invention to provide a composition for promoting oral hygiene comprising turmeric and / or loquat extract as an active ingredient.

Other and further objects of the present invention will be described below.

The present invention relates to a composition for promoting oral hygiene using turmeric and / or loquat extract as an active ingredient, as confirmed in the following Examples and Experimental Examples. The present inventors confirmed the antimicrobial effect on the causative agents of periodontal disease and bad breath by using turmeric extract and loquat extract, and when the turmeric extract and loquat extract were applied in the form of liquid mouthwash, the bacterial membrane formation of the tooth surface was inhibited and gingivitis was improved. It was confirmed. It was also confirmed that turmeric extract and loquat extract inhibited the generation of volatile sulfur compounds, resulting in the bad breath removal effect. The results showed more excellent trends when turmeric and loquat extracts were used together. Therefore, the composition of the present invention may be provided as a composition for promoting oral hygiene having an antimicrobial effect on a bacterium causing oral diseases and thus improving periodontal disease and preventing bad breath.

The present invention has turmeric extract and / or loquat extract as an active ingredient.

Curcuma longa , an active ingredient, is a perennial herbaceous plant of the family Ginger, which grows in South China, India, Okinawa, and Southeast Asia, and is also cultivated in the South Central region of Korea. It has been used for food and dyeing since ancient times and has been known to have medicinal functions such as bile secretion, facilitation of excretion and blood circulation.

Another active ingredient, loquat ( Eriobotrya) japonica ) is a dicotyledonous plant belonging to the rosewood, and is distributed in Korea, Japan, and China. It is known to have antitussive, dry, and diuretic effects, and the substance called amigdaline from the loquat leaves is known to be effective in treating cancer.

As used herein, the term "extract" refers to the root, stem, leaf, flower, fruit, and / or seed or loquat root, stem, leaf, flower, fruit, and / or seed of turmeric regardless of the extraction method. -4 anhydrous or hydrous lower alcohols (such as methanol, ethanol, propanol, butanol, normal-propanol, iso-propanol and normal-butanol), acetone, ethyl acetate, methylene chloride, chloroform, 1,3-butylene glycol, hexane And the extract obtained by extraction with diethyl ether or a mixed solvent thereof and the extract fractionated with the above-mentioned spirit solvent in the extract. Regardless of the extraction method, as long as the extraction object is extracted through the step of immersing the extraction solvent, it should be understood that the extraction method can be applied to any method such as cooling, refluxing, heating, and ultrasonic wave. Nevertheless, the extract is preferably obtained by extracting the object to be extracted with water, ethanol or a mixed solvent thereof, and is meant to include a concentrated liquid extract or solid extract from which the extraction solvent is removed.

As used herein, the term "active ingredient" alone means a component that can exhibit the desired activity or can itself show activity with a carrier which is inactive.

In the present specification, the "oral hygiene-promoting composition" has an antimicrobial effect against oral microorganisms, periodontal disease improvement and bad breath removal effect, and can be understood as "oral cleaning composition".

 As used herein, "improvement" is meant to include treatment, alleviation, and prevention of symptoms.

Terms not specifically defined herein follow the Korean dictionary meaning or meaning commonly used in the art.

The composition of the present disclosure may use turmeric extract and loquat extract, respectively, as an active ingredient, but preferably a mixture of turmeric extract and loquat extract as an active ingredient. As confirmed in the following experimental example, the mixture of turmeric extract and loquat extract shows a greater oral hygiene effect compared to the case of using the extract alone.

The turmeric extract and loquat extract, which are the active ingredients in the composition of the present invention, may be included in any amount (effective amount) depending on the purpose, formulation, and blending purpose, as long as they can exhibit oral hygiene enhancing effects, and a typical effective amount is the total weight of the composition. It will be determined in the range of 0.0001 weight% to 99.9000 weight% based on the above. Here, "effective amount" refers to the amount of the active ingredient that can induce bad breath inhibitory effect. Such effective amounts can be determined experimentally within the ordinary skill of those skilled in the art.

In the composition for promoting oral hygiene of the present invention, the formulation specifically includes all various formulations such as liquid mouthwash, toothpaste, dietary film, mouse spray or chewing gum, and each composition is a conventional method. It can be manufactured by.

When the composition for promoting oral hygiene of the present invention is formulated as a mouthwash, the mouthwash of the present invention contains turmeric extract and / or loquat extract as an essential ingredient, and in addition to wetting agents, foaming agents, preservatives, sweeteners, flavoring agents and other ingredients. And the like.

The humectant may be used by mixing one or more selected from the group consisting of polyhydric alcohols, such as glycerin, sorbitol liquid, amorphous sorbitol liquid, polyethylene glycol, and propylene glycol, as components for inhibiting evaporation of water in the mouthwash composition.

The foaming agent has an emulsifying or solubilizing effect of the water-soluble component and the oil-soluble component in the mouthwashing composition, and has a cleaning effect in the oral cavity, and includes sodium lauryl sulfate, sodium N-lauroyl salcosylate, and N-long-chain acylglutamic acid. It may be used by mixing one or more selected from the group consisting of anionic and nonionic surfactants such as salts, sucrose fatty acid esters, polyoxyethylene hardened castor oil, sorbitan fatty acid esters, and polyoxyethylene polyoxypropylene copolymers.

The preservative may be used as a component for extending the shelf life of the mouthwashes, methyl paraoxybenzoate, benzoic acid, sodium benzoate and the like.

The sweetener and / or flavoring agent is a component for improving the taste and refreshing aftertaste when using the product, and as a sweetening agent, sodium saccharin, aspartame, stevioside, licorice acid, etc. may be used, and as a flavoring agent, peppermint, spearmint oil, Menthol, carbon, anetol, eugenol, etc. can be mixed and used.

As other additives, stabilizers such as sodium citrate for pH control, fluoride agents having caries suppressing function, astringents such as zinc chloride, and food coloring such as blue No. 1 may be used.

Ethanol and the like may be used as a solvent for the composition of the mouthwash, and the remaining components are made up of purified water.

In addition, when the composition for promoting oral hygiene of the present invention is formulated as a toothpaste, the toothpaste of the present invention contains turmeric extract and / or loquat extract as an essential ingredient, in addition to wetting agents, thickeners, foaming agents, preservatives, sweeteners, flavorings And other ingredients.

Thickener among the components of the toothpaste is a component that imparts viscosity to the composition by combining the inorganic powder component and the liquid component insoluble in water in the composition to prevent phase separation over time, carboxymethyl cellulose (CMC), carrageenan, alkyne It may be used by mixing one or more selected from the group consisting of sodium acid, gum arabic, xanthan gum and other gums.

As other additives, it is possible to use abrasives such as calcium phosphate which gives surface luster, fluorine agents having tooth decay function, brighteners such as titanium oxide, and food coloring such as blue No. 1.

In addition, when the composition for promoting oral hygiene of the present invention is formulated as a dietary film, the dietary film of the present invention contains turmeric extract and / or loquat extract as an essential component, and in addition to water-soluble polymers, plasticizers, preservatives, sweeteners. , Fragrances and other ingredients, and the like.

The water-soluble polymer of the dietary film component is hydroxypropyl cellulose (Hydroxypropyl cellulose-Klucel TM), hydroxypropyl methyl cellulose (Hydroxypropyl methylcellulose), hydroxy ethyl cellulose (Hydroxy ethylcellulose), ethyl cellulose (ethylcellulose), carboxymethyl cellulose ( Carboxymethylcellulose, Dextran, gaur-gum, polyvinyl pyrrolidone (PVP), pectin, starch, stacrches, gelatin, casein, sodium alginate, Polyethylene glycol (polyethlyene glycol), polyvinyl glycol (polyvinyl glycol) and the like may be used by mixing one or more selected from the group consisting of.

As the plasticizer, glycols such as propylene glycol or ethylene glycol, glycerin, lecithin, sorbitol, mineral oil, vegetable oil, and the like can be used.

As other additives, an antioxidant, a fluorine agent having tooth decay function, a brightener such as titanium oxide, and an food coloring such as blue No. 1 can be used.

In addition, when the composition for promoting oral hygiene of the present invention is formulated as a mouse spray, the mouse spray of the present invention contains turmeric extract and / or loquat extract as an essential ingredient, and in addition to ethanol, humectant, foaming agent, preservative, sweetener, Fragrances and other ingredients;

As other additives, food colorings such as fluorine and blue No. 1 having caries suppressing function can be used.

In addition, when the composition for promoting oral hygiene of the present invention is formulated as chewing gum, the chewing gum of the present invention contains turmeric extract and / or loquat extract as an essential ingredient, in addition to gum base, plasticizer, foaming agent, preservative, sweetening agent, flavoring agent. And other ingredients.

On the other hand, the composition of the present invention can also be used as a pharmaceutical composition for the prevention or treatment of periodontal disease.

The pharmaceutical composition of the present invention is formulated for oral or parenteral administration by mixing turmeric and / or loquat extract as an active ingredient with a pharmaceutically acceptable carrier, excipient, additive, and the like.

Examples of pharmaceutically acceptable carriers or excipients include lactose, magnesium stearate, starch, talc, gelatin, agar, pectin, gum arabic, olive oil, sesame oil, cacao butter, ethylene glycol and others commonly used.

Examples of the solid composition for oral administration include tablets, pills, capsules, powders, granules and the like. In such a solid composition, the plant extract as an active ingredient is mixed with at least one inert diluent such as lactose, mannitol, glucose, hydroxypropylcellulose, microcrystalline cellulose, starch, polyvinylpyrrolidone, magnesium aluminometasilicate, Mixed. The solid composition may contain additives other than an inert diluent, for example, a lubricant such as magnesium stearate, a disintegrant such as calcium cellulose glycolate, a solubilizing agent such as glutamic acid or aspartic acid, according to a conventional method. The tablets or pills may be coated with a film made of a material such as sucrose, gelatin, hydroxypropylmethylcellulose phthalate or the like, if necessary, and may be coated with two or more layers as the case requires.

The liquid composition for oral administration may contain a generally used inert diluent such as purified water, ethanol, etc., including pharmaceutically acceptable emulsions, solutions, suspensions, syrups, elixirs and the like. The composition may contain, in addition to an inert diluent, a wetting agent, an adjuvant such as a suspending agent, a sweetening agent, a flavoring agent, a fragrance, an antiseptic, and the like.

Injections for parenteral administration include sterile aqueous or non-aqueous solutions, suspensions, and emulsifiers. Examples of aqueous solutions and suspensions include water for injection and physiological saline for injection. Examples of the non-aqueous solution / suspension include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, alcohols such as ethanol, polysorbate 80® and the like. Such a composition may again contain adjuvants such as preservatives, wetting agents, emulsifying agents, dispersing agents, stabilizing agents (for example, lactose), and solubilizing agents (for example, glutamic acid, aspartic acid). These can be sterilized by a conventional sterilization method such as filtration sterilization with a microfiltration membrane, heat sterilization such as high pressure steam sterilization, or sterilizing agent combination. Injections can be solution formulations or freeze-dried preparations which can be used by dissolving in use. Examples of excipients for freeze-drying include sugar alcohols and sugars such as mannitol, glucose and the like.

The pharmaceutical composition of the present invention is administered by an oral administration method or a parenteral administration method.

Preferable examples include parenteral administration methods such as administration by injection (subcutaneous injection, intravenous injection, intramuscular injection, intraperitoneal injection, etc.), transdermal administration, transmucosal administration (transrectal administration, etc.) to be. Of course, oral administration methods may also be used.

The dosage of the active ingredient of the pharmaceutical composition of the present invention can be determined depending on the severity of the disease, the age of the patient, etc., but is generally in the range of 0.005 µg / kg to l00 mg / kg, preferably 0.02 µg / kg to 5 mg / kg. However, since the dosage of the pharmaceutical composition of the present invention is determined in view of various related factors such as route of administration, age, sex, weight, and patient's severity of the patient, the dose is limited in any aspect to the scope of the present invention It should not be understood.

As described above, according to the present invention can provide a composition for promoting oral hygiene using turmeric extract and loquat extract.

Oral hygiene-promoting compositions of the present invention may be commercialized or used as a pharmaceutical composition, such as mouthwash, toothpaste, dietary film, mouse spray or chewing gum.

Hereinafter, the present invention will be described with reference to Examples and Experimental Examples. However, the scope of the present invention is not limited to these examples and experimental examples.

 < Example > Turmeric  Preparation of Extracts and Loquat Extracts

&Lt; Example 1 > Manufacture of turmeric water extract

The turmeric (root) was washed with water and cut at intervals of 5 mm. 1 liter of water was added to 100 g of turmeric, warmed to 65 ° C, extracted for 24 hours, and filtered using Whatman # 1 filter paper. Concentrated under reduced pressure on a water bath below 60 ℃ and using a freeze dryer again to obtain about 11 grams of the extract.

<Example 3> Ethanol Extract Preparation of Turmeric

The turmeric (root) was washed with water and cut at intervals of 5 mm. 1 g of a solvent having a ratio of 3: 7 in water and ethanol was added to 100 g of turmeric, and then extracted for 48 hours in an extraction container, and filtered using Whatman # 1 filter paper. Concentrated under reduced pressure in a water bath below 60 ℃ and again using a freeze dryer to obtain about 13 grams of extract.

Example 2 Preparation of Water Extract of Loquat

After cutting the loquat leaf to 1.0 ~ 2.0cm and added 1 liter of water to 100g and warmed to 65 ℃ extracted for 24 hours. Filter using Whatman # 1 filter paper. Concentrated under reduced pressure on a water bath below 60 ℃ and using a freeze dryer again to obtain about 16 grams of extract.

Example 4 Preparation of Ethanol Extract of Loquat

After cutting the loquat leaf into 1.0 ~ 2.0cm 1 liter of water and ethanol 3: 7 ratio was added to 100g and extracted for 48 hours in the extraction vessel. Filter using Whatman # 1 filter paper. Concentrated under reduced pressure on a water bath below 60 ℃ and again using a lyophilizer to obtain about 19 grams of extract.

The extracts obtained through <Example 1> to <Example 4> were diluted in water at 100 mg / ml and used in experiments or prepared in the form of mouthwashes as in <Preparation Examples 1 to 3 and Comparative Examples> below. It was used for the experiment.

 < Manufacturing example > Preparation of Oral Hygiene Composition

Preparation Examples 1 to 3 and Comparative Examples Preparation of Oral Hygiene Promoting Composition Using Turmeric Extract and Loquat Extract

In the form of a liquid mouthwash in the oral composition, the mouthwashes of <Production Examples 1 to 3 and Comparative Examples> were prepared according to a conventional manufacturing method as shown in Table 1 below. Experimental results of <Experimental Example 1>, because the effect of the ethanol extract was higher than the turmeric and loquat water extract was prepared only mouthwash ethanol extract.

Mouthwash Composition (Unit: weight%) ingredient function Comparative Example Production Example 1 Production Example 2 Production Example 3 Turmeric ethanol extract Active ingredient - 0.2 - 0.1 Loquat ethanol extract Active ingredient - - 0.2 0.1 Sodium fluoride Fluoride 0.2 0.2 0.2 0.2 glycerin Wetting agent 10 10 10 10 Sodium citrate stabilizator 0.5 0.5 0.5 0.5 Polyoxyethylene hardened castor oil Foaming agent
(Solubilizer) One One One One Paraoxybenzoic acid methyl Preservative 0.1 0.1 0.1 0.1 Sodium saccharin Sweetener 0.2 0.2 0.2 0.2 ethanol solvent 7 7 7 7 Purified water menstruum Up to 100 Up to 100 Up to 100 Up to 100

 < Experimental Example > Antibacterial effect, periodontal disease improvement and bad breath control effect experiment

Experimental Example 1 Antimicrobial Effect Experiment of Turmeric Extract and / or Loquat Extract

Representative bacteria causing bad breath and periodontal disease include Porphyromonas gingivali s and Actinobacillus actinomycetem comitans. In order to confirm the antimicrobial activity against these bacteria was measured using a plate medium diffusion method.

Porphyromonas jinjibaris, a causative agent of bad breath and periodontal disease, was incubated anaerobicly at 37˚C using 10% sheep blood, 0.1% hemin and vitamin K1 added trypticase soy agar. Actinobacillus actinomycetemcomitans, the causative agent of periodontal disease, was incubated anaerobicly at 37˚C using trypticase soy agar medium containing 5% horse serum.

To carry out the plate spreading method, porphyromonas jinjibaris or actinobacillus actinomycetemcomitans is plated on a plate, and a sterile paper disk (ADVANTEC, Toyo Roshi Kaisha, Ltd., Japan) with a diameter of 8 mm is plated. It adhered to the medium surface. The turmeric extract or loquat extract of <Example 1> to <Example 4> was diluted in purified water at 100 mg / ml, and the solution was absorbed in sterile paper disk by 20ul or mixed in a 1: 1 ratio. . The result of measuring the inhibition zone (inhibition zone) generated by incubating for 48 hours is shown in the following [Table 2].

(Unit: mm) Strain name Control group Turmeric water extract Turmeric ethanol extract Loquat Water Extract Loquat ethanol extract Turmeric and loquat water extract Turmeric and Loquat ethanol extract Porphyromonas jinjibaris - 6.4 ± 0.2 7.6 ± 0.1 3.0 ± 0.1 5.4 ± 0.3 8.5 ± 0.2 10.2 ± 0.3 Actinobacillus Actinomycetem Comitans - 7.7 ± 0.3 7.8 ± 0.2 5.6 ± 0.2 6.1 ± 0.1 8.1 ± 0.3 11.9 ± 0.1

It was confirmed that turmeric extract and loquat extract had antimicrobial activity through the inhibition of porphymonas jinjibaras, and antibacterial activity was further increased when turmeric extract and loquat extract were treated together. This effect was higher when each extract was treated with an extract extracted with ethanol. In addition, the antibacterial effect on actinobacillus actinomycetem comitans was also high when ethanol extracts of turmeric and loquat were treated together.

Experimental Example 2 Periodontal Disease Improvement Effect Experiment

Before the periodontal disease improvement effect experiment, scaling was performed on all the teeth of the subject and oral examination was performed two weeks later, and this value was set to an initial value. Subjects were given the same toothpaste and toothbrush to make the same conditions, and the mouthwashes of <Preparation Examples 1-3 and Comparative Examples> were used for at least 30 seconds five times a day for 4 to 8 weeks. Ten subjects were included in each group.

<Experimental Example 2-1> Vermiculite bacterium confirmation experiment

The oral hygiene performance index (PHP) was used to determine the oral hygiene effects of turmeric extract and / or loquat extract. After applying the toothpaste to the tooth surface, the degree of discoloration that appears depending on the presence of the dental plaque is confirmed by the numerical value. The degree of dental plaque attachment was scored by the six teeth of the mandibular right first molar, maxillary right middle incisor, maxillary left first molar, mandibular left first molar, mandibular left middle incisor and mandibular right first molar. Each part is divided into five parts: mesial part, centrifugal part, gingival part, center part, and cutting part, and each part is given 0 point if there is no attachment of the bacterial bacterium, and 1 point if the attachment of the dental bacterium is applied. A maximum of 5 points was taken. The results are shown in [Table 3].

Initial value After 4 weeks After 8 weeks Comparative Example 3.12 ± 0.53 3.15 ± 0.67 3.34 ± 0.24 Production Example 1 2.96 ± 0.39 2.75 ± 0.12 2.61 ± 0.32 Production Example 2 3.01 ± 0.47 2.83 ± 0.78 2.72 ± 0.67 Production Example 3 3.07 ± 0.56 2.41 ± 0.43 2.24 ± 0.38

As shown in [Table 3], when the mouthwash containing both turmeric extract and loquat extract was used, it was confirmed that the bacterial membrane of the tooth surface was greatly reduced than when using the comparative example. Through this, it can be seen that the formation of bacterial membrane, which is an early stage of periodontal disease, is inhibited by turmeric extract and loquat extract, and it can be seen that oral hygiene promoting effect is shown.

<Experimental Example 2-2> Experiment to confirm the effect of gingivitis improvement

The Gingival index (GI), designed by Loe and Silness, was used to measure gingivitis. The gums around each tooth were measured and scored using a periodontal probe. 0 point for normal gingival, 1 point for slight color change and mild edema, 2 points for severe inflammation and swelling, color change, bleeding due to periodontal probe, significant redness and swelling, ulceration and continuous bleeding due to severe inflammation If confirmed, three points were given. The results are shown in Table 4 below.

Initial value After 4 weeks After 8 weeks Comparative Example 1.23 ± 0.19 1.18 ± 0.22 1.20 ± 0.24 Production Example 1 1.25 ± 0.22 1.07 ± 0.13 0.88 ± 0.09 Production Example 2 1.19 ± 0.27 1.11 ± 0.28 0.92 ± 0.10 Production Example 3 1.20 ± 0.17 0.89 ± 0.15 0.56 ± 0.21

As shown in the above results, when the turmeric extract and loquat extract were used, respectively, the gingivitis effect was improved, and when the mouthwash containing both the turmeric extract and loquat extract was used, the gingivitis improvement effect was 4 weeks after and 8 weeks after the use. It was confirmed that it was excellent compared with that. As shown in the results of <Experimental Example 2-1> and <Experimental Example 2-2>, it can be seen that turmeric extract and loquat extract inhibit the formation of bacterial membranes on the tooth surface and show the effect of improving gingivitis. have.

Experimental Example 3 Measurement of Bad Breath Control Effect

Twenty patients requiring bad breath management were selected and five oral mouthwashes prepared in <Preparation Examples 1 to 3 and Comparative Examples> were used as test subjects. After the same meal, no action was taken for bad breath and oral care. Bad breath was measured using a Halimeter instrument, which can measure the concentration of volatile sulfur compounds before and after using the mouthwash and after 30 minutes and 3 hours of the mouthwash. For 2 minutes before the measurement, the mouth was closed and breathed only through the nose. During the measurement, the mouth was closed for about 1 minute using a disposable tube connected to the halimeter. When the maximum value of the halimeter was shown, the tube was removed after recording, and the average of the measured values was compared to the degree of bad breath changed by each mouthwash. Bad breath reduction and duration can be calculated by the following equation, and the results are shown in <Table 5>.

Bad breath reduction and duration (%) = (measurement after initial use of mouthwash ÷ initial measurement) × 100

Initial measurement 30 minutes past 3 hours Comparative Example 100 88 102 Production Example 1 100 52 54 Production Example 2 100 61 73 Production Example 3 100 34 35

As a result of confirming the effect of inhibiting bad breath through the above method, 30 minutes after the use of the mouthwash was suppressed by the mouthwash using turmeric extract and 52% compared to the initial measured value and 61% by the mouthwash using the loquat extract. In addition, as the oral cleaning agent added with turmeric and loquat decreased to 34%, it was confirmed that the turbidity and loquat mixture had the highest bad breath inhibitory effect. It was confirmed that the persistence of the inhibitory effect was also excellent.

Claims (7)

Oral hygiene composition comprising both turmeric extract and loquat extract as an active ingredient.
delete The method of claim 1,
The extract is an oral hygiene composition, characterized in that obtained by extracting turmeric or loquat extraction target with water, ethanol or a mixed solvent thereof.
The method of claim 1,
The composition is a composition for promoting oral hygiene, characterized in that for improving periodontal disease.
The method of claim 1,
The composition is a composition for promoting oral hygiene, characterized in that for inhibiting bad breath.

The method according to any one of claims 1 and 3 to 5,
The formulation of the composition is a mouthwash (mouthwash), toothpaste, dietary film, mouse spray or chewing gum, characterized in that the composition for promoting oral hygiene.

The method according to any one of claims 1 and 3 to 5,
The composition is a composition for promoting oral hygiene, characterized in that the pharmaceutical composition.