KR20200050801A - Oral composition containing eugenol and bamboo salt - Google Patents

Abstract

The present invention relates to an oral hygiene composition for preventing and alleviating oral diseases containing eugenol and bamboo salt as active components. According to the present invention, it is possible to provide a useful use of a novel active component which has less side effects, and is safe for the human body while inhibiting the growth of bacteria in the oral cavity. In addition, the present invention provides a composition containing the active component having excellent efficacy as an active component, especially an oral care composition for preventing, alleviating, and treating dental caries, periodontal diseases, and bad breath.

Description

Oral composition containing eugenol and bamboo salt as an active ingredient

The present invention relates to an oral hygiene composition comprising eugenol and bamboo salt as active ingredients.

It has been found that in the oral cavity of humans, 200 to 300 kinds of microorganisms usually reside on the tooth surface, between the teeth and gums of the root, and on the surface of the tongue. The presence of these microorganisms may be a normal phenomenon, but when proper oral hygiene activities are not performed, pathogenic microorganisms cause various oral diseases such as cavities, bad breath, gingivitis, periodontal disease, causing teeth to be lost, health aspects, and aesthetic It has a bad effect on the side.

In general, bacteria of the Streptococcus strain existing in the form of a plaque on the tooth surface form organic acids as their metabolites, and the organic acids formed in this way damage dental hard tissue composed of calcium phosphate and cause tooth decay. On the other hand, anaerobic bacteria existing in the subgingival plaque are known to cause inflammation of the gums by developing into periodontal disease of gingivitis and periodontitis by causing infection in the soft tissues of the gingival area when proper growth conditions are formed. Dental caries and periodontal disease among oral diseases are known as the main factors that cause various clinical symptoms such as pain, chewing function disorder, periodontal tissue destruction, bad breath and pain, and cause dental loss. The causative factor is increasing.

Therefore, in order to alleviate periodontal disease and gum inflammation, the activity of the periodontal pathogen in the subgingival plaque should be suppressed, the blood flow of the inflamed gum should be improved, and the inflammatory reaction in the gum should be suppressed. As a method for inhibiting the proliferation of oral pathogens, various types of antibacterial agents including antibiotics having bactericidal and bacteriostatic action against these bacteria have been developed as inhibitors and treatments for tooth decay, periodontal disease, pulp and root apical infections.

However, antibiotics have the disadvantage that they can be used only as a short-term treatment because it is difficult to use for a long time because it can cause the appearance of resistant bacteria in the oral cavity and systemic side effects along with systemic side effects on our body.

In addition, antibacterial agents used in oral cleansing agents include raw guinarine, listerine, pyroxide, and chlorhexidine, which have an unclear effect on bacteria in the oral cavity and are more unclear about the treatment effect in periodontal disease. Not only does it have a high price but also has a disadvantage in that it is expensive, and Listerine is a major component of alcohol, which has a temporary effect in the oral cavity, but has a slight bacteriostatic effect, but also has a disadvantage in that it may have a harmful effect on tissues when used for a long time. Pyroxide (Peroxide), which is being added for the whitening effect, has a poisonous effect on bacteria, but at the same time has a poisonous effect on human tissues, and also has safety problems, and sometimes bacteria resistant to pyroxide appear in bacteria. In addition, chlorhexidine (Chlorhexidine) is known to be the best among the known formulations as a preventive and therapeutic agent for periodontal disease in addition to inhibition of plaque formation, but it has a strong irritating and strong side effect. In addition to the problems indicated, there are other disadvantages that can be used for a long time for the purpose of treatment or in particular prevention, such as hyphaeosis can be induced, the use is limited in the case of pregnant women due to carcinogenicity (Non-Patent Document 1) .

There is a need for a natural material that is effective in preventing and improving oral diseases such as toothache relief, dental caries, periodontal disease, bad breath, and rash, without concern for the side effects described above.

1. Republic of Korea Patent Publication No. 10-2015-0050995 2. Republic of Korea Patent Publication No. 10-2017-0037229

1. Contemporary periodontics [ed. R.J. Genco et al.], P161-169, p368-369, p433, C.V. Mosby Company, St. Louis, 1990

The present invention is intended to provide an oral hygiene composition that is effective in preventing, improving and treating oral diseases such as toothache relief, dental caries, periodontal disease, bad breath, and syring without concern about the side effects of antibiotics.

In order to achieve the above-mentioned object, the present invention provides an oral hygiene composition for preventing or improving oral diseases, including eugenol and bamboo salt as active ingredients.

According to an aspect of the present invention, the oral hygiene composition of the present invention further comprises Sodium Lauryl Sulfate (SLS).

According to an aspect of the present invention, the oral hygiene composition of the present invention comprises 0.001 to 0.1% by weight of the total weight of the eugenol, and the bamboo salt contains 0.001 to 0.1% by weight of the total weight of the composition.

According to an aspect of the present invention, the oral hygiene composition of the present invention, the eugenol is 0.001 to 0.1% by weight based on the total weight of the composition, the bamboo salt is 0.001 to 0.1% by weight based on the total weight of the composition, and the sodium lauryl sulfate is the total composition 0.001 to 0.1% by weight based on weight.

According to one aspect of the present invention, the oral hygiene composition of the present invention is for preventing, alleviating or treating oral diseases.

According to an aspect of the present invention, the oral disease is composed of bad breath, acute teeth, dental caries, periodontal disease, gingivitis, periodontitis, pulpitis, stomatitis, oral mucositis, intraoral mucosal ulcer, thrush, squamous lichen and other oral inflammation lesions Any one or more diseases selected from the group.

According to an aspect of the present invention, the oral hygiene composition of the present invention has an antibacterial effect in Streptococcus genus or Porphyromonas genus.

According to an aspect of the present invention, the Streptococcus genus or the genus Porphyromonas Streptococcus mutans , Streptococcus sanguis , Streptococcus sanguinis , Streptococcus sanguinis Streptococcus salivarius subsp. Thermophils , Streptococcus sobrinus , Streptococcus ratti , Streptococcus ratti , Streptococcus criceti , Streptococcus criceti One or more selected from the group consisting of Streptococcus anginosus , Streptococcus gordonii and Porphyromonas gingivalis .

The present invention provides a food composition for improving oral hygiene comprising eugenol and bamboo salt as active ingredients.

According to an aspect of the present invention, the food composition for improving oral hygiene of the present invention further comprises sodium lauryl sulfate (SLS).

According to an aspect of the present invention, the food composition for improving oral hygiene of the present invention comprises 0.001 to 0.1% by weight of the total weight of the eugenol, and 0.001 to 0.1% by weight of the bamboo salt based on the total weight of the composition.

According to one aspect of the invention, the food composition for improving oral hygiene of the present invention, the eugenol is 0.001 to 0.1% by weight based on the total weight of the composition, the bamboo salt is 0.001 to 0.1% by weight based on the total weight of the composition, and the sodium lauryl sulfate It contains 0.001 to 0.1% by weight based on the total weight of the silver composition.

According to an aspect of the present invention, the food composition for improving oral hygiene of the present invention is for preventing or alleviating oral diseases.

According to an aspect of the present invention, the oral disease is composed of bad breath, acute teeth, dental caries, periodontal disease, gingivitis, periodontitis, pulpitis, stomatitis, oral mucositis, intraoral mucosal ulcer, thrush, squamous lichen and other oral inflammation lesions Any one or more diseases selected from the group.

According to an aspect of the present invention, the food composition for improving oral hygiene of the present invention has an antibacterial effect in Streptococcus or Porphyromonas.

According to an aspect of the present invention, the Streptococcus genus or the genus Porphyromonas Streptococcus mutans , Streptococcus sanguis , Streptococcus sanguinis , Streptococcus sanguinis Streptococcus salivarius subsp. Thermophils , Streptococcus sobrinus , Streptococcus ratti , Streptococcus ratti , Streptococcus criceti , Streptococcus criceti One or more selected from the group consisting of Streptococcus anginosus , Streptococcus gordonii and Porphyromonas gingivalis .

The present invention provides a pharmaceutical composition for preventing, improving or treating oral diseases comprising eugenol and bamboo salt as active ingredients.

According to an aspect of the present invention, the pharmaceutical composition for preventing, improving or treating oral diseases of the present invention further comprises sodium lauryl sulfate (SLS).

According to an aspect of the present invention, the pharmaceutical composition for preventing, improving or treating oral diseases of the present invention comprises 0.001 to 0.1% by weight of the total weight of the eugenol and 0.001 to 0.1% by weight of the total weight of the composition. do.

According to an aspect of the present invention, the pharmaceutical composition for preventing, improving or treating oral diseases of the present invention is 0.001 to 0.1% by weight of the total weight of the eugenol composition, 0.001 to 0.1% by weight of the total weight of the bamboo salt composition, And the sodium lauryl sulfate comprises 0.001 to 0.1% by weight based on the total weight of the composition.

According to an aspect of the present invention, the pharmaceutical composition for preventing, improving or treating oral diseases of the present invention is for preventing, alleviating or treating oral diseases.

According to an aspect of the present invention, the oral disease is composed of bad breath, acute teeth, dental caries, periodontal disease, gingivitis, periodontitis, pulpitis, stomatitis, oral mucositis, intraoral mucosal ulcer, thrush, squamous lichen and other oral inflammation lesions Any one or more diseases selected from the group.

According to an aspect of the present invention, the pharmaceutical composition for preventing, improving or treating oral diseases of the present invention has an antibacterial effect in the genus Streptococcus or Porphyromonas.

According to an aspect of the present invention, the Streptococcus genus or the genus Porphyromonas Streptococcus mutans , Streptococcus sanguis , Streptococcus sanguinis , Streptococcus sanguinis Streptococcus salivarius subsp. Thermophils , Streptococcus sobrinus , Streptococcus ratti , Streptococcus ratti , Streptococcus criceti , Streptococcus criceti One or more selected from the group consisting of Streptococcus anginosus , Streptococcus gordonii and Porphyromonas gingivalis .

The present invention is a composition derived from nature, confirmed that it shows excellent antibacterial effects against Streptococcus mutans and Porphyromonas gingivalis , which are the major causative agents of oral diseases, and inhibits the pain and inflammation marker PGE2 The effect was confirmed, the formation of gingivitis was suppressed, the suppression of syringin, and the effect of removing bad breath were also confirmed, and it was confirmed that it has excellent prevention, alleviation and treatment effects for various oral diseases without antibiotic side effects.

1 is an experimental result confirming the inhibitory effect on the prostaglandin E2 of the composition of the present invention.

The present invention for achieving the above object is characterized in that it provides an oral hygiene composition comprising eugenol and bamboo salt as an active ingredient. Hereinafter, the present invention will be described in detail with reference to the drawings.

The present inventors conducted a study to derive materials that are effective in preventing and improving various oral diseases without concern for the above-described side effects, and as a result, they are safe in the body and can be used safely and safely. Eugenol (Eugenol) ) And bamboo salt mixture were found to have excellent efficacy against oral diseases.

The eugenol is a phenylpropene having the following structural formula, sperm oil, needlewood oil, cement oil, or in a state free of some oil, or some esters, and is mostly separated from natural products. It is a colorless or pale yellow liquid with a strong carnation-like scent, and the color becomes darker over the storage days. Insoluble in water and soluble in alcohol, ether, chloroform and ethylene glycol.

 [Formula 1]

The eugenol may be used in the form of a biologically acceptable salt, and as the salt, an acceptable alkali addition salt is useful. The expression “biologically acceptable salt” refers to any organic or inorganic addition salt that is a relatively non-toxic and harmless effective action on the subject to be treated and does not adversely affect the beneficial efficacy of eugenol. These salts include alkali metal salts (sodium salt, potassium salt, etc.) and alkaline earth metal salts (calcium salt, magnesium salt, etc.). For example, aluminum, arginine, benzathine, calcium, choline, diethylamine, diolamine, glycine, lysine, magnesium, meglumine, olamine, potassium, sodium, tromethamine, zinc salt, etc. may be included. .

In the oral hygiene composition of the present invention, the eugenol may contain 0.001 to 0.1% by weight, specifically 0.001 to 0.05% by weight, more specifically 0.003 to 0.007% by weight, based on the total weight of the composition, and the bamboo salt is the total weight of the composition Contrast 0.001 to 0.1% by weight, specifically 0.001 to 0.05% by weight, more specifically 0.003 to 0.007% by weight may be included. When the content of each component is less than 0.001% by weight, the effect cannot be effectively exhibited.

In the oral hygiene composition of the present invention, the eugenol and bamboo salt may include 1: 0.01 to 100, specifically 1: 0.1 to 10, and more specifically 1: 0.5 to 1.5 by weight. When included in the above weight ratio, it is possible to effectively exhibit a synergistic effect by the combination of each component.

In the oral care composition of the present invention, sodium lauryl sulfate (SLS) is further included. The sodium lauryl sulfate used in the present invention is an anionic surfactant.

In the oral hygiene composition of the present invention, the eugenol may contain 0.001 to 0.1% by weight, specifically 0.001 to 0.05% by weight, more specifically 0.003 to 0.007% by weight, based on the total weight of the composition, and the bamboo salt is the total weight of the composition It may contain 0.001 to 0.1% by weight, specifically 0.001 to 0.05% by weight, more specifically 0.003 to 0.007% by weight, and the sodium lauryl sulfate is 0.001 to 0.1% by weight relative to the total weight of the composition, specifically 0.001 to 0.05% by weight %, More specifically 0.003 to 0.007% by weight. When the content of each component is less than 0.001% by weight, the effect cannot be effectively exhibited.

In the oral hygiene composition of the present invention, the eugenol: bamboo salt: sodium lauryl sulfate is 1: 0.1 to 10: 0.1 to 10, specifically 1: 0.5 to 1.5: 0.5 to 1.5, more specifically 1: 1: 1 It can be included in a weight ratio of. When included in the above weight ratio, it is possible to effectively exhibit a synergistic effect by the combination of each component.

The oral hygiene composition of the present invention has an excellent antimicrobial effect, PGE inhibitory effect, gingivitis formation inhibitory effect, and syrin has a preventive effect and a bad breath removing effect, and is also effective in preventing and improving oral diseases.

Therefore, the oral hygiene composition of the present invention is for preventing, improving or treating oral diseases. The oral disease to which the oral hygiene composition of the present invention is applied is a comprehensive concept including various oral diseases, for example, but not limited to, bad breath, pain, dental caries, periodontal disease, gingivitis, periodontitis, pusitis, stomatitis , Oral mucositis and oral mucosal ulcer may be any one selected from the group consisting of oral cavity. In addition, the oral disease may include various intraoral diseases that may be caused by external physical stimulation, as well as the effects of microorganisms in the oral cavity, and, for example, expose the ivory tubules due to tooth surface abrasion, indicating hypersensitivity reactions. Syringe may also include symptoms.

As used herein, the term "prevention" may mean any act of suppressing or delaying the disease using the composition for oral hygiene. The oral hygiene composition may be used in the form of pharmaceutical compositions (including quasi-drug compositions) and food compositions, but is not necessarily limited to these forms.

As used herein, the term "improvement" means that the symptoms of oral disease as described above are reduced or softened, and can be understood as a broad concept including alleviation, prevention, and treatment.

As used herein, the term “treatment” is an approach to obtaining beneficial or desirable clinical results, whether detectable or not, which is beneficial or desirable for the purposes of the present invention, whether partial or complete. Regardless of, including, but not limited to, relief of symptoms, reduction of disease severity, stabilized (i.e., no worse) condition of disease, delay or slowing of disease progression, improvement or temporary relief and relief of disease state no. Thus, treatment refers to both therapeutic and prophylactic measures, and those that need to be treated include not only the condition that already has the disease, but also the condition where the disease should be prevented. In addition, by administering the pharmaceutical composition of the present invention to the individual can mean any action to improve or improve the symptoms of oral disease.

As used herein, the term "oral" is intended to mean the entire mouth cavity, including lips, gums (gingiva), hard and soft palate, uvula, palatal tonsils, teeth, inside the cheeks, tongue and tongue papillae. .

As used herein, the term “halt breath” is not limited to this, but due to acquired systemic disease, protein in saliva, food waste, etc., is decomposed by microorganisms in the oral cavity to decompose, resulting in odor. It can be caused by producing substances. The primary cause of bad breath is not limited to microorganisms in the oral cavity, but is not limited to the genus Streptococcus, genus Porphyromonas, Actinomyces genus, Actinobacillus genus, etc. This can be. Typically, an unpleasant odor may be generated in the mouth due to volatile sulfur compounds generated by microorganisms in the oral cavity decomposing proteins.

As used herein, the term “syrinii” means, but is not limited to, all phenomena in which sharp, temporary or persistent pain is exhibited against external stimuli, apart from tooth decay or other pathological causes. The external stimulus usually refers to a temperature stimulus, and usually complains of a symptom in a cold temperature stimulus. In addition to this temperature stimulation, pain may also be caused by stimulation such as drying of the teeth, contact with foreign substances, osmotic pressure through sweet or sour food. It may appear throughout the entire tooth, and may be limited to certain areas such as the maxilla or mandible, or the right or left side, and is accompanied by dentin hyperesthesia, caries, and pulp inflammation. As a cause of this symptom, the exposure of the mouth of the ivory tubule may be considered. When enamel or cementite is destroyed and the entrance of the ivory tubules is exposed to the outside, stimulation is transmitted toward the pulp by the cell processes, nerves and dentin fluid of the ivory tubules, and pain can be felt.

As used herein, the term "tooth caries" refers to dental caries that is caused by damage to the enamel of teeth due to acid generated by the decomposition of sugar and starch by bacteria inhabiting the mouth. The caries can be caused by various causes, for example, plaque, which is a bacterial film formed on the surface of a tooth. When food is consumed, food debris remains in the mouth, and when caries or starch remaining in the mouth is decomposed by bacteria forming the plaque, acid that attacks and damages the enamel on the tooth surface can cause dental caries.

As used herein, the term "periodontal disease" refers to an oral disease including gingivitis and periodontitis, depending on the extent, also known as a trench mouth, but not limited thereto, refers to diseases of the gums and adjacent tissues . Periodontal disease begins with a bacterial infection, and when an inflammatory reaction proceeds with a bacterial toxin, a periodontal pocket is formed between the gum and the tooth. As the disease deepens, the depth of the periodontal pocket deepens and inflammation spreads as a periodontal ligament. Loss occurs. Or, if the balance of bone formation and bone resorption in the alveolar bone is broken and bone resorption exceeds bone formation and the bone mass decreases below the limit, alveolar bone dysplasia, a kind of periodontal disease, occurs. Such alveolar bone formation disorders include alveolar osteoporosis, alveolar osteomalacia, alveolar osteopenia, and alveolar bone remodeling disorder.

As used herein, the term “gingivitis” is a disorder defined as inflammation of the gums and characterized by periodontal disease, the structure of which retains the gums, tissues, dental sacs, and teeth in place. It is characterized by the destruction of the making ligaments. Gingivitis is one of the first stages of serious periodontal disease. Symptoms of gingivitis include swelling gums, mouth soreness, bright red or purple appearance on the gums, shiny gums, painless gums except for touching hands, and bleeding gums.

The term “periodontitis” (Piorrhea alveolaris), as used herein, is inflammation of the periodontal tissue, including tissue supporting the teeth in the oral cavity. The part included in the periodontal tissue is the periodontal ligament or PDL, which consists of the gingival (gingival tissue), the alveolar bone (sockets) to which the teeth are attached, the cementitious or outer layer of the root, and connective tissue fibers connecting the gums and cementitious to the alveolar bone. Symptoms are described as progressive loss of the bone around the tooth that loosens the tooth or loss of the tooth if left unattended. There are various causes for the disease in which bacteria are most common. Periodontitis is considered an advanced form of gum disease because it already involves bone loss in the area. This is the result of neglecting mild gingivitis. Due to bacterial infection, the body reacts negatively and further complications are caused. Periodontitis is one of the leading causes of tooth loss in adults and affects approximately 50% of people over the age of 30. Signs and symptoms appear due to the unstable anchoring of the teeth and the presence of microorganisms. When brushing your teeth with dental floss, biting, chewing, or touching your fingers, the gums often or sometimes become bleeding or turn red. Gum can also swell or pus from time to time. Individuals suffering from periodontitis have bad breath or poor breathing, and there is a bitter taste in the mouth that does not disappear. Partially, teeth may become sharp as the teeth lengthen due to gingival recession, which can be caused by severe brushing. When an enzyme called collagenase begins to destroy collagen, a person will have a deep sac between the teeth and gums. In the early stages of periodontal disease, only a few signs and symptoms may be noticeable. Radical periodontitis can affect individuals at a younger age and may develop symptoms. Some manifestations can be very mild, while others can be very serious. Signs and symptoms usually progress naturally, especially in chronic periodontitis.

The term "pulpitis", as used herein, is inflammation that occurs in the dimensions of the inside of the teeth in which a large number of nerves and blood vessels are distributed, and the pulp tissue is violated by temperature stimulation, chemical stimulation, bacterial stimulation, etc. to necrosis. It means to fall. When inflammatory factors are released in response to harmful stimuli, blood vessels expand and blood flow increases, resulting in tissue edema, unlike normal tissue, in the case of pulp, it exists in a limited space surrounded by hard dentin. The end result will be different from other organizations. On the other hand, it is not limited, but also includes dimensional necrosis in which the dimensional vitality is lost, and dimensional necrosis in which a dimension missing in the necrosis indicates a change in decay.

As used herein, the term “stomatitis” basically refers to oral inflammation, but more specifically, stomatitis is the mucous lining of the oral cavity that can include the gums, tongue, cheeks, lips, and the floor or ceiling of the mouth. (mucous lining). There are various types of stomatitis and classification is based on how a person has acquired the disease. There are two types of stomatitis, contact stomatitis and aphthous stomatitis. Contact stomatitis is inflammation of the oral mucosa caused by contact with allergens or stimulants. Aphthous stomatitis (also known as ulcerative stomatitis or aphthous ulcer) has an unknown etiology. Like contact stomatitis, ulcerative stomatitis affects the oral mucosa. Aphthous ulcer is a type of oral ulcer, which is expressed as a painful open sore inside the mouth or upper throat (including the uvula) caused by mucosal destruction. Aphthous stomatitis is also known as Sutton's Disease, especially in the case of major, ascites or recurrent ulcers. Ulcers are shallow, can be described as individual pain, and are usually observed on neglected mucosal membranes. This type of stomatitis, like contact stomatitis, is self-limiting and generally does not cause complications. The typical size of an ulcer can last 1-2 weeks, while larger ulcers can last for several months.

As used herein, the term "oral mucositis" is a clinical condition in the oral cavity characterized by the presence of a painful manifestation complex and reduced epithelial thickness, intense erythema and ulcers associated with possible development of infection and bleeding. It is understood to mean, and optionally includes stomatitis and oral mucosal ulcers.

As used herein, the term "oral mucosal ulcer" is also referred to as stomatitis, but is not limited to oral mucosa (tongue, gums, lips, cheeks) due to infection by bacteria, viruses, fungi, etc. Etc.), and the bacteria may be bacteria such as the genus Streptococcus, the genus Porphyromonas.

As used herein, the term “oral thrush” is a disease in which the fungus Candida albicans grows in a rapidly uncontrolled manner in the mouth. Bacteria, known as flora, control the growth of Candida albicans in a healthy body. The thrush has a creamy white paste that covers the tongue, and can spread quickly to the palate, gums, back of the throat, tonsils, and inside the cheeks. The symptoms of thrush are white paste that begins to cover the tongue and inside the cheeks. As thrush progresses, mild bleeding may occur if the tongue is rubbed or the patient is brushing his teeth. These symptoms can progress very quickly, but thrush can last for months. If the thrush lesion spreads to the esophagus, the patient may develop additional symptoms such as difficulty swallowing, feeling food in the middle of the throat or chest, and high fever, and the infection can continue to spread through the esophagus.

As used herein, the term "Lichen planus" is often defined as an oral disease that affects the inflamed mouth. Lichen planus is often recognized as a rash that stimulates oral tissue. Although reports of onset are increasing in young patients, most patients have first experience between 45 and 60 years of age. Lichen planus is often associated with the inside of the cheek, but in many cases it appears to affect the entire mouth, including the gums, tongue, and lips, and in rare cases, the throat or esophagus. Lichen planus also occurs as a skin disease on the skin, and is often referred to as a squamous lichen to differentiate it from oral lichen planus. Lichen planus is a self contained disease that can last for weeks, months, and in some cases years. It is not contagious. This is often mistaken for sexually transmitted diseases because the genital organs are most noticeably affected in the early stages of development. Treatment is difficult because symptoms and outbreaks occur quickly and then disappear (often weeks). Some patients get great relief from soaking in cold baths or baths and cryostats, but most patients require medical treatment to relieve symptoms.

As used herein, the term “other oral inflammatory lesions” is caused by other forms of oral inflammation (mucositis, viral, bacterial, fungal or protozoan infections, or due to immune system abnormalities (immunodeficiency, autoimmunity, or allergies). It can be used to treat, including but not limited to, caused oral ulcers. Also included are submucosal fibrosis, chronic debilitating diseases of the mouth characterized by inflammation, and progressive fibrosis of submucosal tissue. This includes glossitis, inflammation of the tongue, or infection.

As used herein, the term "toothache" includes, but is not limited to, pain in the teeth, and generally includes pain in the periodontal tissue supporting the teeth in the jawbone as well as pain in the teeth themselves. When enamel or cementite is destroyed and the entrance of the ivory tubules is exposed to the outside, stimulation is transmitted toward the pulp by the cell processes, nerves and dentin fluid of the ivory tubules, and pain can be felt.

The oral hygiene composition of the present invention has an antibacterial effect in Streptococcus or in Porphyromonas.

In the oral hygiene composition of the present invention, the Streptococcus genus or Porphyromonas genus Streptococcus mutans , Streptococcus sanguis , Streptococcus sanguinis , Streptococcus salivarius species Thermococcus Phelps (Streptococcus salivarius subsp. thermophils), cast tepto Lactococcus small debris Nurse (Streptococcus sobrinus), Streptococcus Lahti (Streptococcus ratti), Streptococcus Cri Shetty (Streptococcus criceti), cast tepto Lactococcus Streptococcus not It may be one or more selected from the group consisting of Streptococcus anginosus , Streptococcus gordonii and Porphyromonas gingivalis . More preferably, the oral hygiene composition of the present invention may exhibit an antibacterial effect against Streptococcus mutans or Porphyromonas gingivalis bacteria.

The oral hygiene composition of the present invention is an active ingredient eugenol and bamboo salt, or in addition to further including SLS, according to the formulation, method of use and purpose of use, additional components, pharmaceutically acceptable or nutritionally acceptable suitable carrier, Excipients, diluents or auxiliary ingredients may be further included.

More specifically, the oral hygiene composition, in addition to the active ingredients, nutrients, vitamins, electrolytes, flavors, colorants, neutralizers, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusting agents, Stabilizers, preservatives, glycerin, alcohol, carbonic acid used in carbonated beverages and the like may be further included. In addition, the carrier, excipient or diluent is lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, aquisia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline In the group consisting of cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil, dextrin, calcium carbonate, propylene glycol, liquid paraffin, and physiological saline It may be any one or more selected, but is not limited thereto, and any common carrier, excipient, or diluent may be used.

The oral hygiene composition, when pharmaceutical, may further include conventional fillers, extenders, binders, disintegrants, surfactants, anticoagulants, lubricants, wetting agents, fragrances, emulsifiers or preservatives, both oral or parenteral Can be used.

Specifically, solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and these solid preparations include at least one excipient such as starch, calcium carbonate, It is prepared by mixing sucrose or lactose and gelatin. In addition, lubricants such as magnesium stearate and talc are used in addition to simple excipients. Liquid preparations for oral use include suspensions, intravenous solutions, emulsions, syrups, etc. In addition to the commonly used simple diluents, water and liquid paraffin, various excipients, such as wetting agents, sweetening agents, fragrances, and preservatives, can be included. .

Forms for parenteral administration include toothpaste, mouthwashes, and topical administration agents (creams, ointments, dressing solutions, sprays, other coatings, etc.). As an example of the formulation of the topical administration agent, the oral hygiene composition containing the active ingredient may be impregnated into a carrier such as gauze made of natural or synthetic fibers.

In the case of the cream or ointment, periodontal disease, gingival disease may be suitable for applying directly to or around the affected area or cavities. In the case of the nebulizer, except for the active ingredient, it may be prepared by a conventional nebulizer preparation method, and may be preventively treated or prevented by treating the compressed container or other spray container with a packing and spraying it frequently to the oral disease site. In the case of the dressing solution, except for containing 2-methoxycinnamaldehyde as an active ingredient, it can be prepared by a conventional method for preparing a dressing solution, and used for dressing in oral disease sites or dressing of other bacterial infection sites. Oral disease can be prevented or treated.

In addition, the formulation of the oral hygiene composition of the present invention may be in a preferred form depending on the method of use, and employs a method known in the art, in particular, to provide rapid, sustained or delayed release of the active ingredient after administration to a mammal. Can be formulated. Examples of specific formulations include warning agents, granules, lotions, linen agents, limonades, powders, syrups, liquids, aerosols, EXTRACTS, elixirs, ointments, fluid extracts, emulsions, suspensions, Premise, acupuncture, tablets, capsules, creams, pills, soft or hard gelatin capsules, and the like.

Further, the oral hygiene composition of the present invention is preferably using a suitable method known in the art or using a method disclosed in Remington's Pharmaceutical Science (Recent Edition), Mack Publishing Company, Easton PA. Can be formulated.

The dosage of the oral hygiene composition according to the present invention may be appropriately selected by a person skilled in the art in consideration of the administration method, the age, sex and weight of the patient, and the severity of the disease. In one example, the oral hygiene composition of the present invention, 2-methoxycinnamaldehyde, 0.0001 mg / kg to 1000 mg / kg, in order to be more effective 0.01 mg / kg to 100 mg / kg can be administered. The administration may be administered once a day, or may be divided into several times. The above dosage does not limit the scope of the present invention in any way.

In addition, the oral hygiene composition of the present invention may further include a compound or a plant extract having a known oral disease inhibitory activity in addition to the above-mentioned active ingredient, and the content may be determined differently according to the production method and purpose of use.

The present invention provides an oral hygiene composition for preventing or ameliorating oral diseases comprising eugenol and bamboo salt as active ingredients, and includes all kinds and formulations that can be used for oral hygiene. Therefore, non-limiting examples of the oral hygiene composition include a food composition and a pharmaceutical composition (including quasi-drug composition), specifically, toothpaste, mouthwash, oral spray, oral ointment, gum, and the like. Further, examples of the food composition of the present invention include food, food additives, beverages or beverage additives.

In the food composition of the present invention, the eugenol may include 0.001 to 0.1% by weight, more specifically 0.001 to 0.05% by weight, more specifically 0.003 to 0.007% by weight, based on the total weight of the composition, and the bamboo salt is compared to the total weight of the composition It may include 0.001 to 0.1% by weight, specifically 0.001 to 0.05% by weight, and more specifically 0.003 to 0.007% by weight. When the content of each component is less than 0.001% by weight, the effect cannot be effectively exhibited.

In the food composition of the present invention, the eugenol and bamboo salt may include 1: 0.01 to 100, specifically 1: 0.1 to 10, and more specifically 1: 0.5 to 1.5 by weight. When included in the above weight ratio, it is possible to effectively exhibit a synergistic effect by the combination of each component.

In the food composition of the present invention, further comprises sodium lauryl sulfate (SLS).

In the food composition of the present invention, the eugenol may include 0.001 to 0.1% by weight, more specifically 0.001 to 0.05% by weight, more specifically 0.003 to 0.007% by weight, based on the total weight of the composition, and the bamboo salt is compared to the total weight of the composition It may contain 0.001 to 0.1% by weight, specifically 0.001 to 0.05% by weight, more specifically 0.003 to 0.007% by weight, and the sodium lauryl sulfate is 0.001 to 0.1% by weight relative to the total weight of the composition, specifically 0.001 to 0.05% by weight , More specifically 0.003 to 0.007% by weight. When the content of each component is less than 0.001% by weight, the effect cannot be effectively exhibited.

In the food composition of the present invention, the eugenol: bamboo salt: sodium lauryl sulfate is 1: 0.1 to 10: 0.1 to 10, specifically 1: 0.5 to 1.5: 0.5 to 1.5, more specifically 1: 1: 1 of It can be included in a weight ratio. When included in the above weight ratio, it is possible to effectively exhibit a synergistic effect by the combination of each component.

Food composition of the present invention exerts a preventive and improving effect on any one or more oral diseases selected from the group consisting of bad breath, acute teeth, dental caries, periodontal disease, gingivitis, periodontitis, pulpitis, stomatitis, oral mucositis and intraoral mucosal ulcers Can be. In addition, the oral disease may include various intraoral diseases that may be caused by external physical stimulation, as well as the effects of microorganisms in the oral cavity, and, for example, expose the ivory tubules due to tooth surface abrasion, indicating hypersensitivity reactions. Syringe may also include symptoms.

The food composition of the present invention has an antibacterial effect in Streptococcus genus or Porphyromonas genus.

In the food composition of the present invention, the Streptococcus genus or Porphyromonas genus Streptococcus mutans , Streptococcus sanguis , Streptococcus sanguinis , Streptococcus sanguinis Streptococcus salivarius subsp. Thermophils , Streptococcus sobrinus , Streptococcus ratti , Streptococcus ratti , Streptococcus criceti , Streptococcus criceti It may be one or more selected from the group consisting of Streptococcus anginosus , Streptococcus gordonii and Porphyromonas gingivalis . More preferably, the food composition of the present invention may exhibit an antibacterial effect against Streptococcus mutans or Porphyromonas gingivalis bacteria.

The food composition of the present invention may further include suitable carriers, excipients, and diluents commonly used in its manufacture.

As used herein, the term "food" refers to a natural product or a processed product that contains one or more nutrients, and preferably means a state that can be directly eaten after some processing process, and is usually used. In the meaning of phosphorus, it includes food, food additives, functional food and beverages.

Foods that may contain the active ingredients of the present invention include, for example, various foods, beverages, chewing gum, candy, tea, vitamin complexes, and functional foods. In addition, in the present invention, the food products include special nutritional foods (e.g., formulas, infants, infant foods, etc.), processed meat products, fish meat products, tofu, jelly, noodles (e.g. ramen, noodles, etc.), health supplements, seasoned foods ( Yes, soy sauce, miso, red pepper paste, mixed sauce, etc., sauces, confectionery (eg, snacks), dairy products (eg, fermented milk, cheese, etc.), other processed foods, kimchi, pickled foods (various kimchi, pickles, etc.), beverages ( Examples include, but are not limited to, fruit, vegetable beverages, soy milk, fermented beverages, ice cream, etc., natural seasonings (eg, ramen soup, etc.), vitamin complexes, alcoholic beverages, alcoholic beverages, and other health supplements. The food, beverage or food additive may be prepared by a conventional manufacturing method.

As used herein, the term "functional food" refers to a biodefense rhythm of a food group or food composition that provides added value to act and express the function of a food product for a specific purpose using physical, biochemical, or biotechnological techniques, etc. Refers to a food product designed and processed to sufficiently express the body control functions related to the regulation, disease prevention and recovery, and the functional food of the present invention preferably provides the body control functions related to the prevention or improvement of oral diseases to the living body. It means food that can express sufficiently. The functional food may include a food-acceptable food supplement additive, and may further include suitable carriers, excipients, and diluents commonly used in the production of functional foods.

As used herein, the term "beverage" means a generic term for drinking to relieve thirst or to enjoy a taste, and is intended to include a functional beverage. The beverage is an essential component in the indicated proportions, and there are no particular limitations on other components other than those containing the active component, and may contain various flavoring agents or natural carbohydrates, etc., as additional components, as in conventional beverages. Examples of the above natural carbohydrates include monosaccharides, such as disaccharides such as glucose and fructose, such as maltose, sucrose, etc., and polysaccharides, such as conventional sugars such as dextrin, cyclodextrin, and the like. Sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those described above, natural flavoring agents (taumatine, stevia extract (for example, rebaudioside A, glycyrrhizine, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The ratio of the natural carbohydrate is generally about 1 to 20 g, preferably 5 to 12 g per 100 ml of the composition of the present invention.Other compositions of the present invention are flesh for the preparation of natural fruit juice, fruit juice beverage, and vegetable beverage. It may further contain.

In addition to the above, the composition of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic flavoring agents and natural flavoring agents, coloring agents and neutralizing agents (cheese, chocolate, etc.), pectic acid and salts thereof, alginic acid and the like. It may contain salts, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohol, carbonic acid used in carbonated beverages, and the like. These components can be used independently or in combination.

As used herein, the term “functional beverage” refers to a biological defense of a beverage group or beverage composition that gives added value to act and express the function of the beverage for a specific purpose by using physical, biochemical, or biotechnological techniques on the beverage. It means a beverage that has been designed and designed to sufficiently express the body control functions for rhythm control, disease prevention and recovery, etc. for the living body.

The functional beverage is an essential component in the indicated proportions, and there is no particular limitation on other components other than those containing the 2-methoxycinnamaldehyde of the present invention, and contains various flavors or natural carbohydrates as additional components, such as ordinary beverages. can do. Examples of the natural carbohydrates include monosaccharides, such as glucose, fructose, and other disaccharides, such as maltose, sucrose, and polysaccharides, such as conventional sugars, such as dextrin, cyclodextrin, and xylitol. , Sugar alcohols such as sorbitol and erythritol. As flavoring agents other than those described above, natural flavoring agents (taumatine, stevia extract (for example, rebaudioside A, glycyrrhizine, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. .

The present invention also provides a pharmaceutical composition for preventing, improving or treating oral diseases, including eugenol and bamboo salt as active ingredients.

In the pharmaceutical composition of the present invention, the eugenol may include 0.001 to 0.1% by weight, more specifically 0.001 to 0.05% by weight, more specifically 0.003 to 0.007% by weight, based on the total weight of the composition, and the bamboo salt is compared to the total weight of the composition It may include 0.001 to 0.1% by weight, specifically 0.001 to 0.05% by weight, and more specifically 0.003 to 0.007% by weight. When the content of each component is less than 0.001% by weight, the effect cannot be effectively exhibited.

In the pharmaceutical composition of the present invention, the eugenol and bamboo salt may include 1: 0.01 to 100, specifically 1: 0.1 to 10, and more specifically 1: 0.5 to 1.5 by weight. When included in the above weight ratio, it is possible to effectively exhibit a synergistic effect by the combination of each component.

In the pharmaceutical composition of the present invention, sodium lauryl sulfate (SLS) is further included.

In the pharmaceutical composition of the present invention, the eugenol may include 0.001 to 0.1% by weight, more specifically 0.001 to 0.05% by weight, more specifically 0.003 to 0.007% by weight, based on the total weight of the composition, and the bamboo salt is compared to the total weight of the composition It may contain 0.001 to 0.1% by weight, specifically 0.001 to 0.05% by weight, more specifically 0.003 to 0.007% by weight, and the sodium lauryl sulfate is 0.001 to 0.1% by weight relative to the total weight of the composition, specifically 0.001 to 0.05% by weight , More specifically 0.003 to 0.007% by weight. When the content of each component is less than 0.001% by weight, the effect cannot be effectively exhibited.

In the pharmaceutical composition of the present invention, the eugenol: bamboo salt: sodium lauryl sulfate is 1: 0.1 to 10: 0.1 to 10, specifically 1: 0.5 to 1.5: 0.5 to 1.5, more specifically 1: 1: 1 of It can be included in a weight ratio. When included in the above weight ratio, it is possible to effectively exhibit a synergistic effect by the combination of each component.

The pharmaceutical composition of the present invention prevents, improves and treats at least one oral disease selected from the group consisting of bad breath, syringis, dental caries, periodontal disease, gingivitis, periodontitis, pulpitis, stomatitis, oral mucositis and intraoral mucosal ulcer. Can exert. In addition, the oral disease may include various intraoral diseases that may be caused by external physical stimulation, as well as the effects of microorganisms in the oral cavity, and, for example, expose the ivory tubules due to tooth surface abrasion, indicating hypersensitivity reactions. Syringe may also include symptoms.

The pharmaceutical composition of the present invention has an antibacterial effect in Streptococcus genus or Porphyromonas genus.

In the pharmaceutical composition of the present invention, the Streptococcus genus or Porphyromonas genus Streptococcus mutans , Streptococcus sanguis , Streptococcus sanguinis , Streptococcus sanguinis Streptococcus salivarius subsp. Thermophils , Streptococcus sobrinus , Streptococcus ratti , Streptococcus ratti , Streptococcus criceti , Streptococcus criceti It may be one or more selected from the group consisting of Streptococcus anginosus , Streptococcus gordonii and Porphyromonas gingivalis . More preferably, the pharmaceutical composition of the present invention may exhibit an antibacterial effect against Streptococcus mutans or Porphyromonas gingivalis bacteria.

The method for preparing a pharmaceutical composition for treating or preventing oral disease of the present invention may be prepared according to a method conventionally manufactured in the industry for manufacturing the same. To this end, in addition to the active ingredient, as a component, typically according to the formulation and purpose of use, we may further include a wetting agent, abrasive, agonist, sweetener, pH adjusting agent, preservatives, binders, flavoring agents, foaming agents and the like.

Wetting agents are used alone or in a mixture of concentrated glycerin, glycerin, sorbitol aqueous solution, amorphous sorbitol aqueous solution, polyethylene glycols and propylene glycol, and the amount used is generally 1 to 70% by weight based on the total weight of the oral hygiene composition. .

Abrasives are selected from the group consisting of precipitated silica, silica gel, zirconium silicate, calcium monohydrogen phosphate, calcium monohydrogen phosphate, hydrous alumina, light calcium carbonate, heavy calcium carbonate, calcium pyrophosphate, insoluble metaphosphate, and aluminum silicate. Etc. can be used. The amount of the abrasive is generally 1 to 60% by weight based on the total weight of the oral hygiene composition. In addition, additives used in small amounts may include sweeteners, pH adjusters, preservatives, colorants, and binders as commonly used components.

Sodium fluoride, sodium monofluorophosphate, sodium fluoride, tin fluoride, chlorohexidine, allantoin chlorohydroxyaluminate, aminocaproic acid, zinc chloride, pyridoxine hydrochloride, tocopherol acetate, enzymes, etc. It can be used by mixing above. Saccharin, xylitol, erythritol, aspartame, or the like can be used as the sweetener, and the amount of the sweetener used is generally 0.05 to 2% by weight based on the total weight of the oral hygiene composition.

As the pH adjusting agent, sodium phosphate, disodium phosphate, citric acid, triethanolamine, and the like can be used. As a preservative, benzoic acid, methylparaben, propylparaben, sodium benzoate, and the like can be used. As a fragrance, peppermint oil, spearmint oil, and menthol may be used in combination, and enzymes such as dextranase may be used as other additives. As the binder, carrageenan, xanthan gum, sodium carboxy methyl cellulose, carboxy vinyl polymer, sodium alginate, laponite, or the like can be used alone or in combination of two or more. As the foaming agent, anionic surfactant sodium lauryl sulfate, copolymer of nonionic surfactant polyoxyethylene polyoxypropylene (poloxamer), polyoxyethylene cured castor oil, polyoxyethylene sorbitan fatty acid ester, etc. may be used. . In addition, it may contain a residual amount of water.

According to another embodiment of the present invention, there is provided an oral hygiene product comprising the above-described composition. The composition for oral hygiene according to the present invention is a composition for oral hygiene that can be generally included in products manufactured and marketed for oral health, and the oral hygiene product is not limited. For example, the product for oral hygiene includes, but is not limited to, toothpaste, oral spray, oral ointment, oral patch, and mouthwash. The product for oral hygiene of the present invention may be in a liquid, solid, suspension, gel, or aerosol form, but is not limited thereto.

When the oral hygiene composition of the present invention is a toothpaste, in addition to containing eugenol and bamboo salt as active ingredients, abrasives, caking agents, moisturizing agents, blowing agents, sweeteners, whitening agents or flavoring agents may be further included. The abrasives include aluminum hydroxide, silicic anhydride, aluminum silicate, dibasic calcium phosphate 2 anhydride and anhydride, tricalcium phosphate, calcium carbonate, calcium pyrophosphate, insoluble sodium metaphosphate, tribasic magnesium phosphate, magnesium carbonate, calcium sulfate, Polymethyl acrylate or the like can be used alone or in combination. The content of the abrasive may be usually 20% to 90% by weight based on the total composition, but is not limited by the content.

When the oral hygiene composition is a paste-like composition, the caking agent is a gum such as garagenin, various thickening cellulose derivatives, xanthan gum, tragacanth gum, polyvinyl alcohol, sodium polyacrylate, polyacrylic acid / An organic caking agent such as a maleic acid copolymer or a synthetic polymer derivative such as a carboxyvinyl polymer, and an inorganic caking agent such as silica or laponite may be used alone or in combination. The content of the caking agent may be usually 0.3% by weight to 5% by weight based on the total composition, but is not limited by the content.

In addition, the oral hygiene composition may further include sorbitol, glycerin, ethylene glycol, propylene glycol, polyether glycol, polypropylene glycol, etc. as a moisturizing agent during its preparation.

In addition, the oral hygiene composition may further include a fragrance or sweetener. The sweetener may be sucrose, lactose, maltose, sorbitol, xylitol, sodium cyclamate, glycerin, sodium saccharin, stevioside, aspartame, etc., and the fragrance may include peppermint, menthol, anethol, cucumbergen, Limonene, citronenol, alpha terpineol, salicylmethyl, cineol, linarol, ethylinarol, vanillin, thymol, spearmint oil, sesame oil, rosemary oil, cinnamon oil, etc., the sweetener or fragrance alone It can be used as or in combination.

In addition, the oral hygiene composition of the present invention may include a surfactant or an additional efficacy component used alone or in combination as a foaming component. The surfactant used as the foaming component may be any one selected from the group consisting of anionic surfactants, nonionic surfactants and cationic surfactants.

When the oral hygiene composition of the present invention is a toothpaste, it can be prepared by a conventional toothpaste manufacturing method except for including the active ingredient, and when the oral hygiene composition of the present invention is a fern solution, in a conventional solution. It can be prepared by mixing 2-methoxycinnamaldehyde and formulating with a fern solution, and can prevent or treat oral diseases by washing the mouth 2 to 10 times a day.

In addition, when the oral hygiene composition of the present invention is a toothpaste cream, a liquid containing water and a moisturizing agent, a gelling agent or a water-insoluble brightener, a sweetener, a fragrance, and the like may be further included.

In addition, when the oral hygiene composition of the present invention is a mouthwash (cleanser), it may further include a toothpaste carrier, more specifically, non-toxic alcohol.

The eugenol and bamboo salt contained in the oral hygiene composition of the present invention is a natural substance and is non-toxic, so it can be used harmlessly and safely to the human body.

Hereinafter, the present invention will be described in more detail through examples. However, these are only for explaining the present invention in more detail, and the scope of the present invention is not limited thereto.

[Experimental Example 1] Antibacterial effect experiment

The paper disk test method is used to determine whether it has the effect of inhibiting strains using Gram-positive bacteria, Streptococcus mutans and Gram-negative bacteria, Porphyromonas gingivalis , which are representative inducing bacteria of dental caries and periodontal disease. Antibacterial activity test was conducted.

Each of the above bacteria, after cultivating the activity under the optimum culture conditions in Table 1, was cultured for 4 to 6 hours in the optimum medium of each bacterium to increase the turbidity of the culture medium in Macfarland turbidity No. After adjusting to 0.5 (1.5x10 ⁸ ), 0.1 ml was spread evenly.

Strain Optimal condition Gram dyeing
(Gram staining) Strain name Temperature badge characteristics Gram (+) Streptococcus mutans 37 ℃ BHI Conditional anaerobic Gram (-) Popiromonas Jinzivalis 37 ℃ TSA Hemin Medanion Medium Anaerobic

The following experimental group materials (reagent grade, Sigma Aldrich) were inoculated at a concentration of 10 mg / disc on a sterile paper disk (Whatman no. 5 paper, 8 mm diameter), and then used after absorption drying for 1 hour. After culturing 24-48 hours at the optimum temperature of each bacterium, the size (diameter mm) of the growth inhibition ring was measured.

The experimental results are shown in Table 2 below.

Treatment material Growth inhibition diameter (mm) Streptococcus mutans Popiromonas Jinzivalis No treatment -(No inhibition) -(No inhibition) Eugenol 9.7 10.0 Bamboo salt 9.5 9.9 SLS 9.9 10.1 Eugenol + bamboo salt (1: 1 mixture) 10.6 10.6 Eugenol + bamboo salt + SLS (1: 1: 1 mixture) 11.4 11.1

As a result of the experiment, in the case of the eugenol + bamboo salt treatment group, even though the total concentration of the final effective substance was the same as 10 mg / disc, especially when the eugenol and bamboo salt treatments were respectively performed (so the concentration of the individual substances was lower than that of the single treatment). Despite this, it was confirmed that the growth inhibition effect was excellent.

[Experimental Example 2] Prostaglandin E2 (PGE2) inhibitory effect

To confirm the effect of alleviating toothache and suppressing inflammation, the inhibitory effect of PGE2, a pain and inflammation marker, was confirmed.

Specifically, macrophages were inoculated at a concentration of 1.5x10 ⁵ cells / ml in DMEM medium containing 10% fetal bovine serum (FBS) and cultured for 24 hours in a 24-well plate at 37 ° C and 5% CO ₂ conditions. In addition, 1 μg / ml of lipopolysaccharide (LPS), which is an inflammation-inducing stimulator, was treated with each substance (eugenol (0.01%), bamboo salt (0.01%), SLS (0.01%), eugenol + bamboo salt (0.005%) + 0.005%), Eugenol + Bamboo salt + SLS (0.00333% + 0.00333% + 0.00333%) The supernatant cultured for 24 hours was evaluated for PGE2 inhibition.

The experimental results are shown graphically in FIG. 1. In the case of the eugenol + bamboo salt treatment group, prostaglandin was inhibited even though the total concentration of the final effective substance was equal to 0.01% (so the concentration of the individual substances was lower than that of the single treatment), especially when the eugenol and bamboo salt treatments were performed respectively. It was confirmed that the effect was excellent.

[Experimental Example 3] Inhibition effect of gingivitis

In order to compare the degree of inhibition of gingivitis, sodium carboxymethylcellulose, sodium lauryl sulfate, glycerin, colloidal silicon dioxide, silicas, sodium cocoyl isethionate, sodium dodecine and sweetener, fragrances , A control toothpaste was prepared using a colorant or the like, and an experimental group toothpaste containing each substance was prepared therein.

Control Contains sodium carboxymethylcellulose, sodium lauryl sulfate, glycerin, colloidal silicon dioxide, silicas, sodium cocoyl isethionate, dodecine and sweetener, fragrance, colorant Experiment group 1 Control + Eugenol 0.01% Experiment group 2 Control + bamboo salt 0.01% Experiment group 3 Control + SLS 0.01% Experimental group 4 Control group + eugenol 0.005% + bamboo salt 0.005% Experimental group 5 Control group + Eugenol 0.0033% + Bamboo salt 0.0033% + SLS 0.0033%

The subjects were gingivitis patients with evenly dentified teeth and no missing teeth. Thirty-seven precise oral checkups were performed according to gender at ages of 30 to 50, followed by screening of 120 subjects and divided into 60. A clinical trial was conducted on the treatment effect of gingivitis.

The test group was divided into an experimental group and a control group to be trained to use 3 times a day after 2 hours after meals, before sleeping, scoring the initial gingivitis index, and using each toothpaste composition for 1 week, 1 month, and 3 After months and 6 months, oral examinations were conducted to examine the gingivitis index. The method of measuring the gingivitis index is by inserting a periodontal probe into the gingival fissure, burning the probe around each tooth without applying force, and measuring the bleeding condition after 30 seconds, as shown in Table 4 below. The scores were recorded by the method to obtain the results in Table 5.

score Contents 0 points No bleeding condition 1 point Normal bleeding condition 2 points Hemorrhage on the ship 3 points Interstitial triangle bleeding condition 4 points Total gingival bleeding condition

division Control Experiment group 1 Experiment group 2 Experiment group 3 Experimental group 4 Experimental group 5 0 weeks 1.05 1.05 1.06 1.05 1.06 1.05 1 week 1.53 1.12 1.19 1.50 1.07 1.05 1 month 2.62 1.15 1.35 2.52 1.09 1.08 3 months 2.82 1.17 1.59 2.59 1.13 1.08 6 months 2.90 1.20 1.70 2.61 1.15 1.09

As a result of the above experiment, after 1 week, the degree of inflammation of the control group was already the most severe with 1.53, and after 6 months, the inflammation was still worsened with 2.90, whereas the eugenol + bamboo salt combination (Experiment 4) and eugenol + bamboo salt of the present invention In the case of + SLS combination (Experimental group 5), it was confirmed that the degree of inflammation was lower than that treated with eugenol alone (Experimental group 1), bamboo salt alone (Experimental group 2), or SLS alone (Experimental group 3).

[Experimental Example 4] Comparison of the degree of suppression of syringin

In order to compare the degree of inhibition of syrin, sodium carboxymethylcellulose, sodium lauryl sulfate, glycerin, colloidal silicon dioxide, silicas, sodium sodium sodium sodium sodium, sodium dodecine and sweetener, fragrance, A control toothpaste was made using a colorant or the like, and an experimental group toothpaste containing each substance was prepared therein. (See Table 3)

The degree of suppression of the symptoms of syringin of the compositions prepared above was comparatively measured. In other words, 40 volunteers who agreed to participate in this experiment as subjects with hypersensitivity teeth were studied, and the total number of teeth studied was 80. In addition, there were 20 men and 20 women among the applicants, and the age ranged from 20 to 50 years. The subjects were not allowed to know the contents of the toothpaste, and the total test period was 2 weeks.

The test was conducted by measuring the patient's response after applying temperature stimulation. Before conducting the test, the hypersensitivity area of each candidate's hypersensitivity teeth was checked in advance, and after dropping about 5 ° C. of cold water into the cold area of the tooth with an eye dropper and scoring, each composition was used 3 times a day for 2 weeks. Then, after 2 weeks, the water was dropped again to a dropper of about 5 ° C with a dropper for evaluation. After stimulation, the criteria for the patient's response rating were set as follows. (0 points: no discomfort, 1 point: slightly uncomfortable or cold, 3 points: sick). Statistical treatment was tested by paired student-t test for stimulation scores before and 2 weeks after laboratory.

The response scores for temperature stimuli after 2 weeks are shown in Table 6 below.

group
time Control Experiment group 1 Experiment group 2 Experiment group 3 Experimental group 4 Experimental group 5 0 weeks 2.14 ± 0.05 2.15 ± 0.10 2.13 ± 0.02 2.14 ± 0.01 2.14 ± 0.12 2.13 ± 0.08 2 weeks 2.15 ± 0.18 1.75 ± 0.20 1.88 ± 0.15 2.00 ± 0.21 1.66 ± 0.02 1.62 ± 0.09 p> 0.05 * p <0.05 * p <0.05 p> 0.05 * p <0.05 * p <0.05

As a result of the experiment, the degree of stimulation of the control group was still 2.15 even after 2 weeks, but there was no significant decrease, whereas in the case of the combination of eugenol + bamboo salt (Experiment 4) and eugenol + bamboo salt + SLS (Experiment 5), respectively The stimulation was reduced to 1.66 and 1.62, which were all significantly lower than the results of Week 0, and it was confirmed that both eugenol alone (Experiment 1), bamboo salt alone (Experiment 2) or SLS alone (Experiment 3) significantly decreased.

[Experimental Example 5] Experiment for removing bad breath

In order to compare the bad breath removal degree, sodium carboxymethylcellulose, sodium lauryl sulfate, glycerin, colloidal silicon dioxide, silicas, sodium cocoyl isethionate, sodium dodecine, etc. A control toothpaste was prepared using a sweetener, fragrance, and colorant, and an experimental group toothpaste containing each substance was prepared therein. (See Table 3)

50 men and women without dental caries were selected and a cross-over test was performed on the target beverage. The commercially available garlic powder was dispersed in water, allowed to stand for 24 hours, and then diluted to obtain a Halimeter measurement value of 700 ppb or more, and the diluted solution was used as a bad breath inducer. Gargle was diluted with 15 ml of garlic powder for 30 seconds, and after 1 minute, bad breath was measured with a halimeter, and then brushed for 30 seconds to 1 minute using a control group or a test group toothpaste. After 1 minute, 5 minutes, and 30 minutes of brushing teeth, the degree of bad breath was measured with a halimeter to measure whether or not to suppress bad breath. The results are shown in the table below.

Bad breath removal effect group
time Control Experiment group 1 Experiment group 2 Experiment group 3 Experimental group 4 Experimental group 5 1 minute 55.8% 96.5% 93.9% 80.0% 98.2% 98.6% 5 minutes 45.1% 86.7% 85.2% 71.4% 89.9% 93.1% 30 minutes 37.2% 83.6% 80.1% 69.7% 86.1% 91.0%

As a result of the experiment, the bad breath inhibition of the control group was 37.2% based on the 30 minutes of the experiment, whereas the combination of eugenol + bamboo salt (Experiment 4) and eugenol + bamboo salt + SLS (Experiment 5) of the present invention was 86.1% and All of them showed excellent bad breath reduction effect as 91%, and it was confirmed that bad breath was reduced when treated with eugenol alone (Experiment 1), bamboo salt alone (Experiment 2) or SLS alone (Experiment 3), which remained at about 69 to 83%. .

Through the above experiment, it can be confirmed that the composition of the present invention has a definite effect as a test composition for effectively maintaining and improving oral health by alleviating toothache, preventing or improving tooth caries, preventing or improving gum disease, bad breath, and reducing the symptoms of syring. have.

In the above, the applicant has described the preferred embodiments of the present invention, but these embodiments are only one embodiment for implementing the technical concept of the present invention and any modification or modification of the present invention as long as the technical concept of the present invention is implemented It should be construed as being within the scope.

Claims (24)

Oral hygiene composition for the prevention or improvement of oral diseases comprising eugenol and bamboo salt as an active ingredient. According to claim 1, Sodium Lauryl Sulfate (Sodium Lauryl Sulfate, SLS) oral hygiene composition for the prevention or improvement of oral diseases further comprising. According to claim 1, The eugenol is 0.001 to 0.1% by weight based on the total weight of the composition, the bamboo salt is oral hygiene composition for the prevention or improvement of oral diseases, characterized in that it comprises 0.001 to 0.1% by weight based on the total weight of the composition. According to claim 2, The eugenol is 0.001 to 0.1% by weight based on the total weight of the composition, the bamboo salt is 0.001 to 0.1% by weight based on the total weight of the composition, and the sodium lauryl sulfate comprises 0.001 to 0.1% by weight based on the total weight of the composition Oral hygiene composition for the prevention or improvement of oral diseases, characterized in that. The oral hygiene composition for preventing or improving oral disease according to claim 1, wherein the composition is for preventing, alleviating or treating oral disease. The method of claim 5, wherein the oral disease is bad breath, pain, dental caries, periodontal disease, gingivitis, periodontitis, pulpitis, stomatitis, oral mucositis, intramucosal ulcers, thrush, squamous lichen and other oral inflammation lesions Oral hygiene composition for the prevention or improvement of oral diseases, characterized in that any one or more diseases selected. The oral hygiene composition for preventing or improving oral diseases according to claim 1, wherein the composition has an antibacterial effect in Streptococcus or Porphyromonas. The method according to claim 1, wherein the genus Streptococcus or the genus Porphyromonas is Streptococcus mutans , Streptococcus sanguis , Streptococcus sanguinis , Streptococcus sanguinis Streptococcus salivarius subsp. Thermophils , Streptococcus sobrinus , Streptococcus ratti , Streptococcus criceti , Streptococcus criceti , Streptococcus angios Streptococcus anginosus ), Streptococcus gordonii (Streptococcus gordonii ) and Porphyromonas gingivalis (Porphyromonas gingivalis ) oral hygiene composition for the prevention or improvement of oral diseases, characterized in that at least one selected from the group consisting of. Food composition for improving oral hygiene comprising eugenol and bamboo salt as active ingredients. The food composition for improving oral hygiene according to claim 9, further comprising Sodium Lauryl Sulfate (SLS). The food composition for improving oral hygiene according to claim 9, wherein the eugenol comprises 0.001 to 0.1% by weight based on the total weight of the composition, and the bamboo salt contains 0.001 to 0.1% by weight based on the total weight of the composition. The method of claim 10, wherein the eugenol is 0.001 to 0.1% by weight based on the total weight of the composition, the bamboo salt is 0.001 to 0.1% by weight based on the total weight of the composition, and the sodium lauryl sulfate comprises 0.001 to 0.1% by weight based on the total weight of the composition. Food composition for improving oral hygiene, characterized in that. The food composition for improving oral hygiene according to claim 9, wherein the composition is for preventing or alleviating oral diseases. 14. The method of claim 13, wherein the oral disease is bad breath, acute teeth, dental caries, periodontal disease, gingivitis, periodontitis, pulpitis, stomatitis, oral mucositis, oral mucosal ulcers, thrush, lichen planus and other oral inflammation lesions Food composition for improving oral hygiene, characterized in that any one or more diseases selected. 10. The method of claim 9, The composition is Streptococcus (Streptococcus) genus or Porphyromonas (Porphyromonas) in the oral hygiene improving food composition characterized in that it has an antibacterial effect. The method according to claim 9, wherein the genus Streptococcus or genus Porphyromonas is Streptococcus mutans , Streptococcus sanguis , Streptococcus sanguinis , Streptococcus sanguinis Streptococcus salivarius subsp. Thermophils , Streptococcus sobrinus , Streptococcus ratti , Streptococcus criceti , Streptococcus criceti , Streptococcus angios Streptococcus anginosus ), Streptococcus gordonii (Streptococcus gordonii ), and Porphyromonas gingivalis (Porphyromonas gingivalis ) at least one selected from the group consisting of food composition for improving oral hygiene. Pharmaceutical composition for the prevention, improvement or treatment of oral diseases comprising eugenol and bamboo salt as active ingredients. The pharmaceutical composition for preventing, improving or treating oral diseases according to claim 17, further comprising Sodium Lauryl Sulfate (SLS). The pharmaceutical composition for preventing, improving or treating oral diseases according to claim 17, wherein the eugenol comprises 0.001 to 0.1% by weight based on the total weight of the composition, and the bamboo salt comprises 0.001 to 0.1% by weight based on the total weight of the composition. The method of claim 18, wherein the eugenol is 0.001 to 0.1% by weight based on the total weight of the composition, the bamboo salt is 0.001 to 0.1% by weight based on the total weight of the composition, and the sodium lauryl sulfate comprises 0.001 to 0.1% by weight based on the total weight of the composition. Pharmaceutical composition for the prevention, improvement or treatment of oral diseases, characterized in that. The pharmaceutical composition for preventing, improving or treating an oral disease according to claim 17, wherein the composition is for preventing, alleviating or treating an oral disease. 22. The method of claim 21, wherein the oral disease is bad breath, pain, dental caries, periodontal disease, gingivitis, periodontitis, pulpitis, stomatitis, oral mucositis, intraoral mucosal ulcer, thrush, lichen planus and other oral inflammation lesions Pharmaceutical composition for the prevention, amelioration or treatment of oral diseases, characterized in that it is any one or more selected diseases. [18] The pharmaceutical composition for preventing, improving or treating oral diseases according to claim 17, wherein the composition has an antibacterial effect in Streptococcus or Porphyromonas. The method of claim 17, wherein the genus Streptococcus or genus Porphyromonas is Streptococcus mutans , Streptococcus sanguis , Streptococcus sanguinis , Streptococcus sanguinis Streptococcus salivarius subsp. Thermophils , Streptococcus sobrinus , Streptococcus ratti , Streptococcus criceti , Streptococcus criceti , Streptococcus angios Streptococcus anginosus ), Streptococcus gordonii (Streptococcus gordonii ), and any one or more selected from the group consisting of Porphyromonas gingivalis (Porphyromonas gingivalis ) pharmaceutical composition for preventing, improving or treating oral diseases.

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