KR102408893B1 - Composition for prevention or treatment of oral disease comprising Pueraria Extract - Google Patents

Abstract

The present invention relates to a composition for preventing, ameliorating or treating oral diseases, comprising a brown extract, and relates to a composition for preventing, improving or treating oral diseases, which is safe and has excellent effects on the human body.

Description

Composition for prevention or treatment of oral disease comprising Pueraria Extract

The present invention relates to a composition for oral care, and relates to a composition that is safe for the human body and is effective in preventing, improving or treating oral diseases such as dental caries, bad breath, and sore throat.

Among oral diseases, dental caries and periodontal disease cause various clinical symptoms such as pain, masticatory dysfunction, destruction of periodontal tissue, bad breath and sore throat, and are known as major factors for tooth loss. The causal factors are increasing.

It is a method of inhibiting the growth of oral pathogens that live in the dental plaque and cause dental caries, periodontal disease, bad breath, and sore throat. , has been developed as an inhibitor and therapeutic agent for pulp and apical infections.

However, since antibiotics can cause the appearance of resistant bacteria in the oral cavity and mycosis mycosis along with systemic side effects on our body, long-term use is difficult, and thus can only be used as a therapeutic agent. In addition, there are antibacterial agents used in mouthwashes such as saengguinarine, listerine, pyroxide, and chlorhexidine. Sangquinarine has an unclear effect on bacteria in the oral cavity, and its therapeutic effect in periodontal disease is more unclear. Not only does it have the disadvantage of being expensive, but Listerine has an alcohol as its main ingredient, and although it has a temporary effect in the oral cavity, it has a slight bacteriostatic effect. Peroxide, which is added for whitening effect, has a toxic effect on bacteria, but at the same time, it also has a toxic effect on human tissues, causing safety problems and sometimes bacteria resistant to pyroxide. In addition, Chlorhexidine is known to be the best known agent among agents known to date as a preventive and therapeutic agent for periodontal disease along with the inhibition of plaque formation. Besides, there are disadvantages in that it cannot be used for a long period of time for the purpose of treatment or, in particular, prevention, such as can cause mycosis mycosis, and restrict its use in pregnant women due to its carcinogenicity.

On the other hand, a toothache symptom or a symptom called tooth hypersensitivity refers to a toothache symptom when eating sour fruit or drinking cold beverages. When this occurs, the dentinal tubules protected by the gums are exposed. In order to improve these symptoms, toothpaste for the prevention of sore throat is being developed, and treatment is performed to fill the exposed dentinal tubules with resin, etc. at the dentist. However, in the case of a toothpaste specializing in toothpaste that utilizes the sealing effect of the conventional dentin tubule, it contains a fine powder of the active ingredient to seal the dentin tubule. Usually, it is diluted quickly in the process of brushing, and most of the toothpaste is washed away in the process of rinsing with water. There was a problem in that the customs was not sealed and exposed again.

Accordingly, the inventors of the present inventors have conducted research to derive ingredients that are effective in preventing and improving oral diseases, such as dental caries, bad breath, and sore throat, while not having any concerns about side effects and harmless to the human body.

KR 10-2013-0105622 A (2013.09.25)

Therefore, the present invention is to solve the above problems, and to provide a novel active ingredient capable of inhibiting the growth of bacteria in the oral cavity while having fewer side effects and being safe for the human body, that is, to provide such a useful use of the active ingredient.

In other words, the problem to be solved by the present invention is to provide a composition comprising an active ingredient having excellent efficacy as an active ingredient, in particular, a composition for oral care for preventing or improving dental caries and bad breath.

An object of the present invention is to provide a composition for treating or improving oral diseases using natural ingredients that are safe and can be used safely in the body.

An object of the present invention is to provide a composition for oral use that can relieve symptoms of soreness by forming a coating film on the surface of a damaged tooth using a natural material.

In order to solve the above problems, the present invention provides a composition for oral use derived from a natural substance. Specifically, in an embodiment of the present invention, there is provided a composition for oral use comprising brown ( Pueraria ) as an active ingredient.

That is, the present invention provides a new use of a composition for oral use containing brown algae, particularly a composition for oral use for preventing, improving or treating various oral diseases.

Researchers of the present invention have conducted research for the development of oral compositions that are harmless to the human body and have effects on the treatment, prevention, improvement, etc. of oral diseases And it was confirmed that it can have a tooth surface coating effect, and completed the present invention. Through the continuous research process of the inventors of the present invention, it was possible to identify for the first time the effect of treating or preventing oral diseases of the brown extract of the present invention.

The brown flower is arrowroot ( Pueraria ). lobata Ohwi) or a flower just starting to bloom, and preferably, dried arrowroot bud may be used for the brown flower.

The efficacy and effect are different for each part of the plant, and the brown flower of the present invention does not have a bitter taste unlike the root and leaves of arrowroot, so it is suitable for use in oral compositions, and has excellent antibacterial activity compared to other parts of arrowroot. , it has been confirmed as a result of long-term studies that Sirin has excellent preventive and ameliorating effects. In particular, it was confirmed that there is an excellent antibacterial effect against tooth decay-causing bacteria.

The method of using the brown algae is not particularly limited, and may be included in the oral composition as a powder after drying, and preferably may be included in the oral composition as an extract.

As used herein, the term "extract" refers to an extract obtained by extraction treatment of brown rice, a diluted or concentrated liquid of the extract, a dried product obtained by drying the extract, a prepared or purified product of the extract, or a mixture thereof, etc., It includes extracts of all formulations that can be formed using the extract itself and the extract.

In the brown seaweed extract of the present invention, the method for extracting the brown seaweed is not particularly limited, and may be extracted according to a method commonly used in the art. Non-limiting examples of the extraction method include hot water extraction, ultrasonic extraction, filtration, reflux extraction, and the like, and these may be performed alone or in combination of two or more methods, preferably using hot water extraction. can be done by

In the present invention, the type of the extraction solvent used for extracting the browning is not particularly limited, and any solvent known in the art may be used. Non-limiting examples of the extraction solvent include water, distilled water, methanol, ethanol, propanol, isopropanol, butanol, acetone, ether, benzene, chloroform, ethyl acetate, methylene chloride, n-hexane, hydrochloric acid, acetic acid, formic acid, diethyl ether and one or more solvents selected from the group consisting of cyclohexane, and the like. When alcohol is used as the solvent, preferably C ₁ to C ₄ alcohols (methanol, ethanol, propanol, butanol) may be used. In the present invention, more preferably, water, ethanol, or a mixture thereof may be used as the extraction solvent for the browning.

In the present invention, the composition may be for preventing, improving or treating oral diseases.

In the present invention, the oral disease is a comprehensive concept including, for example, but not limited to, bad breath, dental caries, and mucosal ulcers in the oral cavity that may be caused by microorganisms in the oral cavity.

In addition, the oral disease may include various diseases in the oral cavity that may be caused by external physical stimuli as well as the influence of microorganisms in the oral cavity. A sore throat may also include symptoms.

As used herein, the term 'treatment' in the present invention is an approach for obtaining beneficial or desirable clinical results, for the purposes of the present invention, whether beneficial or desirable clinical results are detectable or not possible, and also includes, whether partial or total, alleviation of symptoms, reduction of the severity of the disease, a stabilized (i.e., not worsening) state of the disease, delaying or reducing the rate of disease progression, amelioration or temporary alleviation and amelioration of the disease state; However, the present invention is not limited thereto. Accordingly, treatment refers to both therapeutic treatment and prophylactic dimensions, and those that need to be treated include those already having the disease as well as those in which the disease is to be prevented. In addition, it may refer to any action to improve or benefit the symptoms of oral disease by administering the pharmaceutical composition of the present invention to an individual.

"Prevention" may refer to any action of inhibiting or delaying a disease using the composition for oral use. The oral composition may be used in the form of a pharmaceutical composition, a food composition, or a composition for oral hygiene, but is not necessarily limited to this form.

In addition, as used herein, the term "improvement" refers to the reduction or alleviation of symptoms such as the above, and may be understood as a broad concept including alleviation, prevention, treatment, and the like.

As used herein, the term "halitosis" may mean a symptom of an unpleasant odor from the mouth, but is not limited thereto, but is typically caused by volatile sulfur compounds generated when microorganisms in the oral cavity decompose proteins. It can cause an unpleasant odor from the mouth.

As used herein, the term "dental caries" refers to dental caries in which tooth enamel is damaged by acid, which is generated when sugar, starch, etc. is decomposed by bacteria living in the mouth, resulting in tooth decay. The caries may be caused by a variety of causes, for example, may be a bacterial film formed on the surface of the tooth, such as plaque (plaque). When food is ingested, food residues remain in the mouth, and when the bacteria that make up the plaque break down sugar or starch remaining in the mouth, acid, which is generated by attacking the enamel of the tooth surface, can cause dental caries.

As used herein, the term “mouth mucosal ulcer” is also called stomatitis, but is not limited thereto, but is not limited thereto, due to infection by bacteria, viruses, fungi, etc. in the oral cavity (tongue, gums, lips, inside the cheeks) etc.) may include diseases that cause inflammation.

The oral disease may be caused by bacteria, and the bacteria may be bacteria of the genus Streptococcus.

The composition for oral use of the present invention is a genus of Streptococcus, more specifically, Streptococcus mutans, Streptococcus sanguis, Streptococcus salicyrus sanguinis, and Species thermophils (Streptococcus salivarius subsp. thermophils) has an excellent antibacterial effect on one or more bacteria selected from the group consisting of, it can have an excellent effect in the prevention, improvement or treatment of oral diseases.

More preferably, the composition for oral use of the present invention may exhibit an antibacterial effect on Streptococcus mutans bacteria.

According to another embodiment of the present invention, the brown extract of the present invention is included in a composition for preventing, ameliorating, or treating shirini symptoms.

As used herein, the term “shirin-i” is not limited thereto, but refers to any phenomenon in which sharp, temporary, or continuous pain appears in response to external stimuli, regardless of dental caries or other pathological causes. The external stimulus usually means a temperature stimulus, and usually complains of a symptom to a cold temperature stimulus, but pain may also appear in a hot temperature stimulus. In addition to such temperature stimulation, pain may also appear due to stimulation such as dry teeth, contact with external substances, or osmotic pressure through sweet or sour food. It may appear on the entire tooth, or it may appear limited to a specific area such as the maxilla or mandible, or the right or left side, and may be accompanied by dentine hyperesthesia, caries, or pulp inflammation.

Exposure of the entrance to the dentinal tubule may be considered as the cause of such palpitation symptoms.

When the enamel or cementum is destroyed and the entrance of the dentinal tubule is exposed to the outside, the stimulation is transmitted to the pulp by the cell projections, nerves and dentin fluid of the dentinal tubule, and pain can be felt.

The inventors of the present invention confirmed that the brown extract can form a coating film on the tooth surface by reacting with calcium ions contained in saliva, and the formed coating film prevents exposure of the dentinal tubule to prevent, improve, or treat sore throat symptoms did The composition for preventing or treating oral diseases of the present invention can be directly applied to animals including humans. The animal is a biological group corresponding to the plant, which mainly consumes organic matter as nutrients, and has differentiated digestion, excretion and respiratory organs, and may be preferably a vertebrate, more preferably a mammal. Specifically, the mammal may be a human, a pig, a cow, or a goat. The composition for preventing or treating oral diseases may include the brown algae extract alone as an active ingredient, and additional ingredients, that is, pharmaceutically acceptable or nutritionally acceptable carriers, excipients, depending on the formulation, method of use, and purpose of use. , a diluent or an accessory ingredient may be further included.

In more detail, the composition for preventing or treating oral diseases is a nutritional supplement, vitamins, electrolytes, flavoring agents, coloring agents, thickeners, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloid thickeners in addition to the active ingredients. , a pH adjuster, a stabilizer, a preservative, glycerin, alcohol, a carbonation agent used in carbonated beverages, and the like may be additionally contained. In addition, the carrier, excipient or diluent may be lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia gum, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline From the group consisting of cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil, dextrin, calcium carbonate, propylene glycol, liquid paraffin, physiological saline It may be one or more selected, but the present invention is not limited thereto, and any conventional carrier, excipient or diluent may be used.

The composition for preventing or treating oral diseases may further include conventional fillers, extenders, binders, disintegrants, surfactants, anti-aggregants, lubricants, wetting agents, fragrances, emulsifiers or preservatives, etc. Or both parenteral can be used.

Specifically, solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and these solid preparations include at least one excipient in the brown extract, for example, starch, calcium carbonate, It is prepared by mixing sucrose or lactose, gelatin, etc. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid formulations for oral use include suspensions, solutions, emulsions, syrups, etc., and various excipients such as wetting agents, sweeteners, fragrances, and preservatives in addition to commonly used simple diluents such as water and liquid paraffin may be included. .

Forms for parenteral administration include toothpaste, mouthwash, topical administration agents (creams, ointments, dressing solutions, sprays, other coating agents, etc.). An example of the formulation of the topical administration agent may be one in which a composition for preventing or treating oral diseases including an active ingredient, a brown extract, is impregnated into a carrier such as gauze made of natural or synthetic fibers.

In the case of the above cream or ointment, it may be suitable for direct application to or around periodontal disease, gingival disease affected area or caries area. In the case of the spray, it can be prepared by a conventional spray preparation method, except that it contains brown algae extract as an active ingredient, and it is filled and packaged in a compressed container or other spray container and sprayed frequently to the area of oral disease to prevent oral disease. Or it can be treated. In the case of the dressing solution, it can be prepared by a conventional dressing solution manufacturing method except for containing brown algae extract as an active ingredient. can be prevented or treated.

In addition, the formulation of the composition for preventing or treating oral disease of the present invention may be in a preferred form depending on the method of use, and in particular, it is known in the art to provide rapid, sustained or delayed release of the active ingredient after administration to a mammal. It can be formulated by adopting an established method. Examples of specific formulations include warning agents, granules, lotions, liniments, limonades, powders, syrups, liquids, aerosols, extracts, elixirs, ointments, fluid extracts, emulsions, suspensions, There are pills, tablets, capsules, creams, pills, soft or hard gelatin capsules.

Furthermore, the composition for preventing or treating oral disease of the present invention can be prepared by using an appropriate method known in the art or by Remington's Pharmaceutical Science (Remington's Pharmaceutical Science (recent edition), Mack Publishing Company, Easton PA). It can be preferably formulated using

The dosage of the composition for preventing or treating oral disease according to the present invention may be appropriately selected by those skilled in the art in consideration of the administration method, the age, sex and weight of the user, and the severity of the disease. As an example, the composition for preventing or treating oral diseases of the present invention may be administered in an amount of 0.0001 mg/kg to 1000 mg/kg, and 0.01 mg/kg to 100 mg/kg to be more effective. Administration may be administered once a day, or may be administered in several divided doses. The above dosage does not limit the scope of the present invention in any way.

In addition, the composition for preventing or treating oral diseases of the present invention may further include a compound or plant extract having known oral disease inhibitory activity in addition to the brown extract, and the content may be determined differently depending on the manufacturing method and purpose of use.

In an embodiment of the present invention, there is also provided a food composition for preventing or improving oral diseases comprising a brown algae extract as an active ingredient. Examples of the food composition of the present invention include food, food additives, beverages or beverage additives.

The food composition comprising the brown algae extract as an active ingredient may further include suitable carriers, excipients and diluents commonly used in the preparation thereof.

In the present specification, food means a natural product or processed product containing one or more nutrients, and preferably means a state that can be eaten directly through some processing process, and in a conventional sense, It is intended to include all foods, food additives, health functional foods and beverages.

Foods to which the brown extract of the present invention can be added include, for example, various foods, beverages, gums, candy, tea, vitamin complexes, functional foods, and the like. In addition, in the present invention, food includes special nutritional food (eg, formula milk, infant food, etc.), processed meat products, fish meat products, tofu, jelly, noodles (eg, ramen, noodles, etc.), health supplements, seasoned foods ( Ex, soy sauce, soybean paste, red pepper paste, mixed soy sauce, etc.), sauces, sweets (eg snacks), dairy products (eg fermented milk, cheese, etc.), other processed foods, kimchi, pickled foods (various kimchi, pickles, etc.), beverages ( Examples include, but are not limited to, fruit and vegetable beverages, soy milk, fermented beverages, ice cream, etc.), natural seasonings (eg, ramen soup, etc.), vitamin complexes, alcoholic beverages, alcoholic beverages and other health supplements. The food, beverage or food additive may be prepared by a conventional manufacturing method.

Functional food in the present invention is a food group or food composition that has added value to act and express the function of the food for a specific purpose using physical, biochemical, and bioengineering methods, etc. It means a food that is designed and processed to sufficiently express the body control function related to the body, and preferably, the functional food of the present invention can sufficiently express the body control function related to the prevention or improvement of oral disease to the living body. means food. The functional food may include a food supplementary additive that is pharmaceutically acceptable, and may further include an appropriate carrier, excipient and diluent commonly used in the manufacture of functional food.

In the present invention, beverage means a generic term for drinking to quench thirst or enjoy taste, and is intended to include functional beverages. The beverage is not particularly limited in other components other than containing the brown algae extract as an active ingredient as an essential ingredient in the indicated ratio, and may contain various flavoring agents or natural carbohydrates as additional ingredients like a conventional beverage. Examples of the above natural carbohydrates include monosaccharides, such as glucose, fructose, etc. disaccharides, such as maltose, sucrose, etc. and polysaccharides, such as conventional sugars such as dextrin, cyclodextrin, and the like, and These are sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those described above, natural flavoring agents (taumatin, stevia extract (eg, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The proportion of the natural carbohydrate is generally about 1 to 20 g, preferably 5 to 12 g, per 100 ml of the composition of the present invention. In addition, the composition of the present invention is the pulp for preparing natural fruit juice, fruit juice beverage, and vegetable beverage. may further contain.

In addition to the above, the composition of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic and natural flavoring agents, coloring agents and thickeners (cheese, chocolate, etc.), pectic acid and its salts, alginic acid and its It may contain salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated beverages, and the like. These components may be used independently or in combination.

Functional beverage in the present invention refers to a beverage group or beverage composition that has added value to act and express the function of the beverage for a specific purpose using physical, biochemical, and bioengineering methods, etc. It refers to a beverage designed and processed to sufficiently express body control functions related to recovery, etc.

The functional beverage is not particularly limited in other ingredients except for containing the brown algae extract of the present invention as an essential ingredient in the indicated ratio, and may contain various flavoring agents or natural carbohydrates as additional ingredients like a conventional beverage. Examples of the natural carbohydrate include monosaccharides, such as glucose, fructose, etc. disaccharides, such as maltose, sucrose, and the like, and polysaccharides, such as conventional sugars such as dextrin, cyclodextrin, and the like, and xylitol. , a sugar alcohol such as sorbitol and erythritol. As flavoring agents other than those described above, natural flavoring agents (taumatin, stevia extract (eg, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. .

The present invention provides an oral hygiene composition for preventing or improving oral diseases, comprising a brown algae extract as an active ingredient. The oral care composition of the present invention includes all types and formulations that can be used for oral hygiene. Non-limiting examples of the oral care composition include toothpaste, mouthwash, oral spray, oral ointment, gum, and the like.

When the oral hygiene composition of the present invention is a toothpaste, an abrasive, a binder, a moisturizer, a foaming agent, a sweetener, a whitening agent, or a flavoring agent may be additionally included in addition to including the brown extract as an active ingredient. The abrasive includes aluminum hydroxide, silicic anhydride, aluminum silicate, dicalcium phosphate dihydrate and anhydride, tricalcium phosphate, calcium carbonate, calcium pyrophosphate, insoluble sodium metaphosphate, magnesium triphosphate, magnesium carbonate, calcium sulfate, Polymethyl methacrylate and the like may be used alone or in combination. The content of the abrasive may be usually 20 wt% to 90 wt% based on the total composition, but is not limited by the content.

When the oral care composition is a paste-like composition, the binder is garrageenin, various cellulose derivatives for thickening, xanthan gum, gums such as tragacanth gum, polyvinyl alcohol, sodium polyacrylate, polyacrylic acid / An organic binder such as a synthetic polymer derivative such as a maleic acid copolymer or a carboxyvinyl polymer and an inorganic binder such as silica or laponite may be used alone or in combination. The content of the binder may be usually 0.3 wt% to 5 wt% based on the total composition, but is not limited by the content.

In addition, the oral hygiene composition may further include sorbitol, glycerin, ethylene glycol, propylene glycol, polyether glycol, polypropylene glycol, and the like as a moisturizing agent during the preparation thereof.

In addition, the oral hygiene composition may further include a flavoring agent or a sweetener. The sweetener may be sucrose, lactose, maltose, sorbitol, xylitol, sodium cyclamate, glycerin, sodium saccharin, stevioside, aspartame, and the like, and the flavoring agent is peppermint, menthol, anethol, eugenol, It may be limonene, citronenol, alphaterpineol, salicylmethyl, cineol, linalol, ethylinarol, vanillin, thymol, spearmint oil, sesame oil, rosemary oil, cinnamon oil, etc., and the sweetener or flavoring agent is alone may be used as or in combination.

In addition, the oral hygiene composition of the present invention may contain a surfactant used as a foaming component or an additional effective component alone or in combination. The surfactant used as the foaming component may be any one selected from the group consisting of anionic surfactants, nonionic surfactants and cationic surfactants.

When the oral hygiene composition of the present invention is a toothpaste, it may be prepared by a conventional toothpaste manufacturing method except for including brown algae extract as an active ingredient. It can be prepared by mixing brown algae extract and formulating it as a toothbrushing solution, and can prevent or treat oral diseases by washing the oral cavity 2 to 10 times a day.

In addition, when the oral hygiene composition of the present invention is a toothpaste cream, it may further include a liquid including water and a moisturizing agent, a gelling agent or a water-insoluble brightener, a sweetener, a flavoring agent, and the like.

In addition, when the oral care composition of the present invention is a mouthwash (cleansing agent), it may further include a toothpaste carrier, more specifically, non-toxic alcohol.

The method for preparing the composition for treating or preventing oral disease of the present invention may be prepared according to a method conventionally prepared in the industry for manufacturing the same.

In the present invention, the brown extract may be present in an amount of 0.001 to 10% by weight based on the total weight of the composition, preferably 0.005 to 5% by weight based on the total weight of the composition, and more preferably 0.008 to 1% by weight based on the total weight of the composition. % can be When the brown extract is less than 0.001% by weight relative to the total weight of the composition, the effect on oral diseases may be insignificant.

The composition for oral use of the present invention may further include a wetting agent, an abrasive, a medicinal agent, a sweetener, a pH adjuster, a preservative, a binder, a fragrance, a foaming agent, etc. .

As a wetting agent, concentrated glycerin, glycerin, sorbitol aqueous solution, amorphous sorbitol aqueous solution, polyethylene glycol, and propylene glycol are used alone or in combination, and the amount used is 1 to 70% by weight based on the total weight of the oral composition.

The abrasive is one selected from the group consisting of precipitated silica, silica gel, zirconium silicate, calcium monohydrogen phosphate, anhydrous calcium monohydrogen phosphate, hydrous alumina, light calcium carbonate, ground calcium carbonate, calcium pyrophosphate, insoluble metaphosphate and aluminum silicate. etc. can be used. The amount of the abrasive is generally 1 to 60% by weight based on the total weight of the composition for oral use. In addition, the additive used in a small amount may include a sweetener, a pH adjuster, a preservative, a colorant, and a binder as commonly used components.

The active agent is sodium fluoride, sodium monofluorophosphate, sodium fluoride, tin fluoride, chlorhexidine, allantoin chlorohydroxyaluminate, aminocaproic acid, zinc chloride, pyridoxine hydrochloride, tocopherol acetate, enzymes, etc. alone or in two types. It can be used by mixing the above.

As the sweetener, saccharin, xylitol, erythritol, aspartame, etc. can be used, and the amount of the sweetener is generally 0.05 to 2% by weight relative to the total weight of the oral composition.

As a pH adjuster, sodium phosphate, disodium phosphate, citric acid, triethanolamine, etc. can be used.

As the preservative, benzoic acid, methylparaben, propylparaben, sodium benzoate and the like can be used. As a flavoring agent, peppermint oil, spearmint oil, menthol, etc. may be mixed and used, and enzymes such as dextaranase may be used as other additives.

As a binder, carrageenan, xanthan gum, sodium carboxymethyl cellulose, carboxyvinyl polymer, sodium alginate, laponite, etc. may be used alone or in combination of two or more.

As the foaming agent, sodium lauryl sulfate as an anionic surfactant, a copolymer of polyoxyethylene polyoxypropylene as a nonionic surfactant (poloxamer), polyoxyethylene hydrogenated castor oil, polyoxyethylene sorbitan fatty acid ester, etc. may be used. .

In addition, it may contain a residual amount of water.

According to another embodiment of the present invention, there is provided a product for oral hygiene comprising the composition. The composition for oral hygiene according to the present invention containing brown extract as an active ingredient is a composition for oral hygiene that can be included in all products manufactured and marketed for general oral health, and there is no limitation on the product for oral hygiene to be applied. For example, the oral hygiene products include, but are not limited to, toothpaste, oral spray, oral ointment, oral patch, and mouthwash. The oral hygiene product of the present invention may be in the form of a liquid, solid, suspension, gel, or aerosol, but is not limited thereto.

The composition containing the brown extract of the present invention provides an effect of preventing, improving or treating oral diseases.

The present invention provides a composition for oral use derived from a natural material that has few side effects, is safe for the human body, and can inhibit the growth of bacteria in the oral cavity.

It is possible to provide a composition that is not toxic and has excellent stability while exhibiting excellent prevention or improvement of dental caries and bad breath.

It can exhibit excellent effects in the prevention and treatment of dental caries.

Hereinafter, in order to explain the present invention in more detail, the following examples and the like will be described. However, the embodiments according to the present invention may be modified in various other forms, and the scope of the present invention should not be construed as being limited to the embodiments described below. The embodiments of the present invention are provided by way of example to help the specific understanding of the present invention.

<Preparation of brown flower extract>

The brown extract of the present invention was prepared in the following way.

The brown flowers were minced, immersed in 95% ethanol solution, and extracted at room temperature for 48 hours to obtain a brown seaweed extract.

<Experiment 1. Antibacterial effect test>

Antibacterial activity was tested using the paper disc test method to see if there was an effect in inhibiting the strain using the gram-positive bacterium Streptococcus mutans, which is a representative bacterium causing dental caries. After culturing the above-mentioned bacteria in the optimal culture conditions of Table 1 below, incubate for 4 to 6 hours in the optimal medium of each bacteria to determine the turbidity of the culture medium by Macfarland turbidity No. After adjusting so as to be 0.5 (1.5x10 ⁸ ), 0.1ml was spread evenly. The brown extract was inoculated at a concentration of 10 mg/disc on a sterilized paper disk (Whatman no. 5 paper, 8 mm diameter), and then absorbed and dried for 1 hour before use. After culturing for 24-48 hours at the optimum temperature of the above bacteria, the size (diameter mm) of the growth inhibition ring was measured.

strain optimum condition Gram staining strain name temperature badge characteristics Gram (+) Streptococcus mutans 37℃ BHI Facultative anaerobic

The results are shown in the table below.

Measurement result of antibacterial activity against S. mutans of brown flower extract

Growth inhibition ring diameter (mm) unprocessed - (No inhibition) Brown algae extract treatment 11.5

The S. mutans inhibitory effect was confirmed by treating the brown algae extract, and the caries reduction effect was confirmed.

<Experiment 2. Comparison of the degree of suppression of irritability>

In order to compare the degree of inhibition of sirin, first, sodium carboxymethylcellulose, sodium lauryl sulfate, glycerin, colloidal silicon dioxide, silicas, sodium cocoylethionate sodium, which can be generally used for preparing toothpaste, A control toothpaste was prepared using dodicin, a sweetener, a fragrance, a coloring agent, and the like, and an experimental group toothpaste containing 0.01% by weight of brown extract was prepared.

The degree of suppression of irritation of the above-prepared compositions was comparatively measured. In other words, 40 volunteers who agreed to participate in this experiment as people with hypersensitive teeth were included in the study, and the total number of teeth in the study was 80. Also, among the applicants, 20 were male and 20 were female, and the age ranged from 20 to 50 years old. The subjects of the study did not know the contents of the toothpaste, and the total test period was 2 weeks.

The test was conducted by measuring the patient's response after applying a temperature stimulus. Prior to the test, each volunteer's hypersensitive tooth was checked in advance, and each composition was used 3 times a day for 2 weeks after rating by dropping cold water at about 5° C. After 2 weeks, cold water of about 5°C was dropped again with a dropper to evaluate. After stimulation, the patient's response rating criteria were set as follows (0 points: no discomfort, 1 point: slight discomfort or irritation, 3 points: pain). For statistical processing, the stimulus scores before and 2 weeks after the laboratory were tested with the paired student-t test.

The response scores to the temperature stimulus after 2 weeks are shown in Table 3 below.

Degree of inhibition of sirin

army
hour control experimental group 0 weeks 2.14±0.19 2.14±0.06 2 weeks 2.15±0.04 1.55±0.28 p>0.05 *p<0.05

As can be seen in Table 3 above, it was found that the reaction to temperature stimulation was much smaller in the experimental group to which the brown extract was administered. This shows that sirin had an excellent inhibitory effect in the group to which the brown extract was administered, and the ingredient contained in the brown extract of the present invention met with the calcium component contained in saliva to form a coating film on the tooth surface, resulting in tooth irritation hypersensitivity symptoms could alleviate

<Experiment 3. Halitosis Removal Effect Experiment>

Sodium carboxymethylcellulose, sodium lauryl sulfate, glycerin, colloidal silicon dioxide, silicas, sodium cocoylethionate sodium, dodicin and sweeteners that can be generally used to prepare toothpaste to compare the degree of removal of bad breath , fragrance, colorant, etc. were used to make a control toothpaste, and an experimental group toothpaste containing 0.01% by weight of brown extract was prepared.

50 men and women without dental caries were selected and a cross-over test was performed on the target beverage. Commercially available garlic powder was dispersed in water and allowed to stand for 24 hours, then diluted so that the Halimeter measurement value was 700 ppb or more, and the diluted solution was used as a source of bad breath. After gargling with 15ml of garlic powder diluted solution for 30 seconds, the degree of bad breath was measured 1 minute later with a Hallimeter. After 1, 5, and 30 minutes of brushing, the degree of bad breath was measured with a Hallimeter to determine whether or not the suppression of bad breath was continued. The results are shown in the table below.

bad breath removal effect

army
hour control experimental group 1 minute 55.9% 96.8% 5 minutes 41.2% 87.9% 30 minutes 36.8% 82.4%

[meaning]

It was confirmed that the brown flower extract showed the effect of improving dental caries and removing bad breath. In addition, by improving the degree of hypersensitivity to temperature stimulation, Shirin gave a relief effect.

The brown extract of the present invention is included in the oral composition to effectively maintain and improve oral health, and it is believed that it will give a certain effect.

Claims (14)

An oral hygiene composition for preventing or improving dental caries caused by the genus Streptococcus , comprising a brown ethanol extract as an active ingredient. delete delete The oral care composition for preventing or improving dental caries according to claim 1, wherein the brown ethanol extract is included in an amount of 0.001 to 10% by weight based on the total weight of the oral care composition. delete delete delete The method of claim 1, wherein the Streptococcus ( streptococcus ) genus Streptococcus mutans ( streptococcus mutans ), Streptococcus sanguis ), Streptococcus saligunis spp . salivarius subsp. thermophils ) Oral hygiene composition for preventing or improving dental caries, characterized in that at least one selected from the group consisting of. delete The prevention of dental caries according to claim 1, wherein the oral care composition has any one or more formulations selected from the group consisting of toothpaste, mouthwash, oral spray, oral ointment, oral patch, and gum. , or oral hygiene composition for improvement. A food composition for preventing or improving dental caries induced by the genus Streptococcus comprising a brown ethanol extract as an active ingredient. delete A pharmaceutical composition for preventing, improving or treating dental caries caused by the genus Streptococcus , comprising an ethanol extract of brown ethanol as an active ingredient. delete