KR102632336B1 - Composition for prevention or treatment of dental disease comprising an extract of Daphne genkwa - Google Patents

Abstract

The present invention relates to a composition for preventing, treating or improving oral diseases containing red bean flower extract. The composition for preventing, treating or improving oral diseases of the present invention can increase the production of sallyberry mucin and can be used safely without concerns about resistance or resistance that may be caused by excessive use of antibacterial substances.

Description

Composition for prevention or treatment of dental disease comprising an extract of Daphne genkwa}

The present invention relates to a composition for preventing or treating oral diseases, and more specifically, to a composition that can effectively prevent or treat oral diseases by activating saliva secretion in the oral cavity.

In oral health, salivary mucin present in saliva plays a very important role. Specifically, sallyberry mucin is known to prevent oral dryness, protect the oral cavity from environmental stress, lubricate the oral cavity, and bind to pathogens to inhibit attachment of pathogens to teeth. Therefore, people with low production of sallyberry mucin, which is responsible for oral health, are relatively more likely to be exposed to oral diseases.

Oral diseases such as dental caries, gingivitis, periodontitis, bad breath, etc. are fundamentally caused by Streptococcus mutans. mutans ), Porphyromonas gingivalis , Prevotella intermedia ( Prevotella ) intermedia ), Actinobacillus Actinomycetemcomitans ( Actinobacillus actinomycetemcomitans ), and Candida albicans ( Candida It is known to be mainly caused by oral pathogens of the Candida spp., including pathogenic microorganisms such as albicans ).

Therefore, in the past, research on methods to inhibit or kill the growth of oral pathogens or on antibacterial substances has been mainly conducted to treat oral diseases. However, most of these antibacterial substances remove not only harmful pathogens in the oral cavity, but also beneficial bacteria in the oral cavity. Due to excessive use of antibacterial substances, oral pathogens that become resistant due to increased resistance to antibacterial substances or genetic mutations occur. As the number of diseases increases, there are limits to the prevention or treatment of oral diseases using only conventional antibacterial substances. And in the case of dry mouth, if the current pilocarpine medication is administered for several weeks or more, symptoms of dryness and atrophy of the oral mucosa are improved. However, this drug is a non-specific muscarinic agonist and can cause side effects such as sweating, nausea, dizziness, flushing, and lethargy by increasing the parasympathetic nervous system, so it has limitations as a way to prevent or improve dry mouth.

Therefore, the present invention solves the above problems and aims to provide a useful use of the ingredient, that is, an ingredient that can effectively prevent or treat oral diseases through other actions in addition to antibacterial activity.

In other words, the problem to be solved by the present invention is to provide a composition that is excellent in preventing or treating oral diseases by containing as an active ingredient a component that promotes the production of saliva in the oral cavity, more specifically, salivary mucin. do.

In order to solve the above problems, the present invention provides a composition for preventing or treating oral diseases containing an extract of red bean flower ( Daphne genkwa ), preferably the red bean flower extract promotes the production of sallyberry mucin present in saliva to prevent or treat oral diseases. A composition with excellent effectiveness is provided.

In order to develop a material that can prevent and treat oral diseases with activities other than antibacterial activity, the inventors of the present invention were researching a material that promotes the production of mucin using salivary gland cells that produce mucin components, and discovered red bean flower ( Daphne ) It was confirmed that the genkwa ) extract promotes the production of celery mucin present in saliva.

Accordingly, through continuous research, the inventors of the present invention found that the red bean flower extract was effective in oral health and completed a composition for preventing, improving, or treating oral diseases using the extract.

The red bean flower ( Daphne genkwa ) extract of the present invention may be an extract obtained by extracting red bean flowers using water, ethanol, propylene glycol, etc., preferably water, ethanol, or a mixture thereof as an extraction solvent. Preferably, the red bean flower extract of the present invention may be an extract extracted from the flower buds of the red bean flower. Red bean flower extract is known to be effective in diuretic action, expectoration, or deworming, but there is still no known effect of red bean flower extract on treating or preventing oral diseases.

The red bean flower extract of the present invention can promote the production of sallyberry mucin present in saliva and can have a therapeutic or preventive effect on oral diseases. Specifically, it was confirmed that red bean flower extract has the effect of preventing and improving oral diseases by promoting the production of sallyberry mucin. The use of the red bean flower extract for preventing or treating oral diseases has not yet been known, and through the continuous research process of the inventors of the present invention, the effect of the red bean flower extract for treating or preventing oral diseases was identified for the first time.

While the inventors of the present invention were conducting research on ways to prevent or treat oral diseases with activities other than antibacterial activity, they discovered that the red bean flower extract can activate sallyberry mucin production and completed the present invention. It has been done.

The term “prevention” used in the present invention refers to all actions that suppress or delay the onset of oral disease by administering the pharmaceutical composition of the present invention to an individual.

The term “treatment” used in the present invention refers to all actions that improve or benefit symptoms of oral disease by administering the pharmaceutical composition of the present invention to an individual.

In the present invention, the oral disease is a concept that includes all diseases that occur in the oral cavity regardless of the condition, but for the purpose of the present invention, it may preferably mean an oral disease caused by a decrease in oral immunity. Non-limiting examples of the oral diseases include dental caries, gingivitis, periodontitis, oral mucous membrane ulcers, bad breath, dry mouth, or dry teeth.

The red bean flower extract may be included in the composition in the amount necessary within the range capable of achieving the purpose of the present invention, and the content is not particularly limited.

According to one embodiment of the present invention, the oral disease refers to an oral disease including any one selected from the group consisting of periodontitis, gingivitis, cavities, oral mucous membrane ulcers, bad breath, dry mouth, and sensitive teeth. The oral disease may preferably be gingivitis, periodontitis, cavities, dry mouth or dry mouth, more preferably gingivitis, periodontitis, dry mouth or dry mouth, and most preferably dry mouth.

In one embodiment of the present invention, gingivitis is a disease in which inflammation occurs in the gums, which are part of the oral mucosa, and symptoms of swelling, redness, pain, or local heat appear, and in severe cases, ulcers or gangrene may occur. it means. Salivary, especially salivary mucin, contained in saliva plays an important role in oral health. The sallyberry mucin can protect the oral mucosa, neutralize toxicity, and maintain appropriate acidity. The red bean flower extract of the present invention can protect the oral mucosa by activating the production of sallyberry mucin, and can be expected to have the effect of reducing the occurrence of gingivitis and alleviating gingivitis.

In one embodiment of the present invention, periodontitis is also called periperiodontitis and is a disease that mainly occurs in the gums and bony tissue. When it becomes a chronic infection, periodontal disease causes tooth loss due to destruction of cell tissue. It means disease. The red bean flower extract protects the oral mucosa by activating celery mucin, neutralizes toxicity, and maintains appropriate acidity, thereby exhibiting a gum protection effect and toxicity alleviation effect, thereby preventing or treating periodontitis.

In another embodiment of the present invention, tooth decay (or dental caries) refers to an oral disease in which tooth decay bacteria break down sugar and convert it into acid, destroying the enamel or dentin of the tooth, causing holes in the teeth and blackening. . The red bean flower extract can protect the tooth surface by activating sallyberry mucin, and through this, it can also exhibit a cavity prevention effect.

In an embodiment of the present invention, dry mouth refers to a case where salivary secretion in the basic state is less than 0.1 ml per minute. Healthy adults secrete 1 to 1.5 liters of saliva per day, but if less saliva is produced, the mouth feels dry. A dry mouth alone can make it difficult to swallow food, cause a sore throat, and can also cause digestive problems. Additionally, dry mouth can worsen oral diseases such as bad breath, gingivitis, and cavities. The red bean flower extract can increase salivary secretion and prevent dry mouth by activating celery mucin.

In another embodiment of the present invention, tooth sensitivity is also called tooth hypersensitivity, and refers to a case in which teeth are sensitive to external stimuli such as temperature stimulation, or the symptom persists for a long time and develops into pain in severe cases. . The red bean flower extract can prevent or treat the symptoms of sensitive teeth by activating sallyberry mucin to protect the tooth surface and oral mucosa.

The composition for preventing or treating oral diseases of the present invention can be directly applied to animals, including humans. The animal refers to a group of organisms corresponding to plants, which mainly consumes organic matter as nutrients and has differentiated digestive, excretory and respiratory organs, and is preferably a vertebrate, and more preferably a mammal. Specifically, the mammal may be a human, pig, cow, or goat. The composition for preventing or treating oral diseases may contain the red bean flower extract alone as an active ingredient, and may contain additional ingredients, such as pharmaceutically acceptable or nutritionally acceptable carriers and excipients, depending on the formulation, method of use, and purpose of use. , may additionally contain diluents or auxiliary ingredients.

More specifically, the composition for preventing or treating oral diseases includes, in addition to the active ingredients, nutrients, vitamins, electrolytes, flavors, colorants, thickening agents, pectic acid and its salts, alginic acid and its salts, organic acids, and protective colloidal thickeners. , pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonating agents used in carbonated beverages, etc. may be additionally contained. In addition, the carrier, excipient or diluent may include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, gum aquisia, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline. From the group consisting of cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil, dextrin, calcium carbonate, propylene glycol, liquid paraffin, and physiological saline. There may be one or more selected ones, but it is not limited thereto, and all common carriers, excipients, or diluents can be used.

When the composition for preventing or treating oral diseases is pharmaceutically formulated, it may further contain conventional fillers, extenders, binders, disintegrants, surfactants, anti-coagulants, lubricants, wetting agents, flavorings, emulsifiers or preservatives, and may be administered orally. Alternatively, it can be used parenterally.

Specifically, solid preparations for oral administration include tablets, pills, powders, granules, capsules, etc. These solid preparations include the red bean flower extract with at least one excipient, such as starch, calcium carbonate, It is prepared by mixing sucrose, lactose, and gelatin. Additionally, in addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid preparations for oral use include suspensions, oral solutions, emulsions, syrups, etc. In addition to the commonly used simple diluents such as water and liquid paraffin, various excipients such as wetting agents, sweeteners, fragrances, and preservatives may be included. .

Forms for parenteral administration include toothpaste, mouthwash, topical administration (cream, ointment, dressing solution, spray, other coating agents, etc.). An example of the formulation of the topical administration agent may be one in which the active ingredient, red bean flower extract, or a composition for preventing or treating oral diseases is impregnated into a carrier such as gauze made of natural fiber or synthetic fiber.

The cream or ointment may be suitable for direct application to or around areas affected by periodontal disease, gingivitis, or cavities. In the case of the above spray, it can be manufactured by a conventional spray manufacturing method except that it contains red bean flower extract as an active ingredient, and is filled and packaged in a compression container or other spray container and sprayed and applied to oral disease areas from time to time to prevent oral disease. Or it can be treated. In the case of the dressing solution, it can be prepared by a conventional dressing solution manufacturing method except that it contains red bean flower extract as an active ingredient, and can be used as a dressing for an oral disease site or a dressing for other bacterial infection sites to prevent oral infectious diseases. It can be prevented or treated.

In addition, the formulation of the composition for preventing or treating oral diseases of the present invention may be in a desirable form depending on the method of use, and is known in the art to provide rapid, sustained or delayed release of the active ingredient, especially after administration to a mammal. It can be formulated by adopting a known method. Examples of specific dosage forms include powders, granules, lotions, liniments, limonade, powders, syrups, solutions, aerosols, EXTRACTS, elixirs, ointments, fluid extracts, emulsions, suspensions, There are whole products, infusions, tablets, capsules, creams, pills, and soft or hard gelatin capsules.

Furthermore, the composition for preventing or treating oral diseases of the present invention can be prepared using an appropriate method known in the art or a method disclosed in Remington's Pharmaceutical Science (recent edition), Mack Publishing Company, Easton PA. It can be preferably formulated using.

The dosage of the composition for preventing or treating oral diseases according to the present invention can be appropriately selected by a person skilled in the art in consideration of the administration method, the age, gender and weight of the recipient, and the severity of the disease. For example, the composition for preventing or treating oral diseases of the present invention can be administered at 0.0001 mg/kg to 1000 mg/kg, and for more effectiveness, at 0.01 mg/kg to 100 mg/kg, based on the red bean flower extract. there is. Administration may be administered once a day, or may be administered several times. The above dosage does not limit the scope of the present invention in any way.

In addition, the composition for preventing or treating oral diseases of the present invention may further include compounds or plant extracts with known oral disease inhibitory activity in addition to the red bean flower extract, and the content may be determined differently depending on the manufacturing method and purpose of use.

One embodiment of the present invention also provides a food composition for preventing or improving oral diseases containing red bean flower extract as an active ingredient. Examples of the food composition of the present invention include food, food additives, beverages, or beverage additives.

The food composition containing the red bean flower extract as an active ingredient may further include appropriate carriers, excipients, and diluents commonly used in its production.

In this specification, food refers to a natural product or processed product containing one or more nutrients, preferably in a state that can be eaten directly after a certain degree of processing. In the usual sense, It is intended to include all foods, food additives, health functional foods and beverages.

Foods to which the red bean flower extract of the present invention can be added include, for example, various foods, beverages, gum, candy, tea, vitamin complexes, functional foods, etc. Additionally, in the present invention, foods include special nutritional foods (e.g., infant formula, infant/toddler food, etc.), processed meat products, fish products, tofu, jelly, noodles (e.g., ramen, noodles, etc.), health supplements, and seasoned foods ( (e.g., soy sauce, soybean paste, red pepper paste, mixed paste, etc.), sauces, confectionery (e.g., snacks), dairy products (e.g., fermented milk, cheese, etc.), other processed foods, kimchi, pickled foods (various kimchi, pickles, etc.), beverages (e.g. Examples include, but are not limited to, fruits, vegetable beverages, soy milk, fermented beverages, ice cream, etc.), natural seasonings (e.g., ramen soup, etc.), vitamin complexes, alcoholic beverages, alcoholic beverages, and other health supplements. The food, beverage or food additive can be manufactured by conventional manufacturing methods.

In the present invention, functional food refers to a food group or food composition in which added value is added to the food to function and express the function of the food for a specific purpose using physical, biochemical, biotechnological methods, etc., regulating the biological defense rhythm, disease prevention and recovery. It refers to a food designed and processed to sufficiently express body regulatory functions related to the body to the living body. Preferably, the functional food of the present invention is a food that can sufficiently express the body regulatory function related to the prevention or improvement of oral diseases to the living body. It means food. The functional food may include food auxiliary additives that are foodologically acceptable, and may further include appropriate carriers, excipients, and diluents commonly used in the production of functional foods.

In the present invention, beverage refers to a general term for drinking to quench thirst or enjoy taste, and is intended to include functional beverages. The drink has no particular restrictions on other ingredients other than including the red bean flower extract as an essential ingredient in the indicated ratio, and may contain various flavoring agents or natural carbohydrates as additional ingredients like a regular drink. Examples of the above natural carbohydrates include common sugars such as monosaccharides, such as glucose and fructose, disaccharides such as maltose, sucrose, etc., and polysaccharides, such as dextrins, cyclodextrins, etc., and Sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (thaumatin, stevia extract (e.g., rebaudioside A, glycyrrhizin, etc.)) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The ratio of the natural carbohydrate is generally about 1 to 20 g, preferably 5 to 12 g, per 100 ml of the composition of the present invention. In addition, the composition of the present invention is used as pulp for the production of natural fruit juice, fruit juice beverage, and vegetable beverage. It may additionally contain.

In addition to the above, the composition of the present invention contains various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic and natural flavors, colorants and thickening agents (cheese, chocolate, etc.), pectic acid and its salts, alginic acid and its May contain salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonating agents used in carbonated beverages, etc. These ingredients can be used independently or in combination.

In the present invention, a functional beverage refers to a group of beverages in which added value is added to the beverage to act and express the function of the beverage for a specific purpose using physical, biochemical, or biotechnological techniques, or a beverage composition that provides biological defense rhythm regulation, disease prevention, and other benefits. It refers to a beverage designed and processed to fully express body regulation functions related to recovery, etc. to the living body.

The functional beverage has no particular restrictions on other ingredients other than containing the red bean flower extract of the present invention as an essential ingredient in the indicated ratio, and may contain various flavoring agents or natural carbohydrates as additional ingredients like a conventional beverage. Examples of the natural carbohydrates include monosaccharides, such as glucose, fructose, etc., disaccharides, such as maltose, sucrose, etc., and polysaccharides, such as common sugars such as dextrin, cyclodextrin, etc., and xylitol. Sugar alcohols such as sorbitol and erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (thaumatin, stevia extract (e.g., rebaudioside A, glycyrrhizin, etc.)) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. .

The present invention provides an oral hygiene composition for preventing or improving oral diseases containing red bean flower extract as an active ingredient. The oral hygiene composition of the present invention includes all types and formulations that can be used for oral hygiene. Non-limiting examples of the oral hygiene composition include toothpaste, mouthwash, oral spray, oral ointment, gum, etc.

As used herein, the term "improvement" means any action that results in at least a reduction in the severity of a parameter, such as a symptom, associated with the condition being treated.

When the oral hygiene composition of the present invention is a toothpaste, in addition to containing red bean flower extract as an active ingredient, it may further contain an abrasive, binder, moisturizer, foaming agent, sweetener, whitening agent, or flavoring agent. The abrasives include aluminum hydroxide, anhydrous silicic acid, aluminum silicate, dicalcium phosphate dihydroxide and anhydrous, tricalcium phosphate, calcium carbonate, calcium pyrophosphate, insoluble sodium metaphosphate, tribasic magnesium phosphate, magnesium carbonate, calcium sulfate, Polymethyl methacrylate, etc. can be used alone or in combination. The content of the abrasive may typically be 20% to 90% by weight based on the total composition, but is not limited to the content.

When the oral hygiene composition is a paste-like composition, the binder may include garagenine, various cellulose derivatives for thickening, xanthan gum, gums such as tragacanth gum, polyvinyl alcohol, sodium polyacrylate, polyacrylic acid/ Organic binders such as synthetic polymer derivatives such as maleic acid copolymers and carboxyvinyl polymers and inorganic binders such as silica and laponite can be used alone or in combination. The content of the binder may generally be 0.3% by weight to 5% by weight based on the total composition, but is not limited by the content.

In addition, the oral hygiene composition may further include sorbitol, glycerin, ethylene glycol, propylene glycol, polyether glycol, polypropylene glycol, etc. as moisturizers during production.

Additionally, the oral hygiene composition may additionally contain flavoring agents or sweeteners. The sweetener may be sucrose, lactose, maltose, sorbitol, xylitol, sodium cyclamate, glycerin, sodium saccharin, stevioside, aspartame, etc., and the flavoring agent may be peppermint, menthol, anethole, eugenol, It may be limonene, citronol, alpha-terpineol, salicylmethyl, cineol, linalol, ethyllinalol, vanillin, thymol, spearmint oil, sage oil, rosemary oil, cinnamon oil, etc., and the sweetener or flavoring agent may be used alone. It can be used alone or in combination.

Additionally, the oral hygiene composition of the present invention may contain surfactants used as foaming ingredients or additional effective ingredients alone or in combination. The surfactant used as the foaming ingredient may be any one selected from the group consisting of anionic surfactants, nonionic surfactants, and cationic surfactants.

If the oral hygiene composition of the present invention is a toothpaste, it can be prepared by a conventional toothpaste manufacturing method except that it contains red bean flower extract as an active ingredient, and if the oral hygiene composition of the present invention is a toothbrushing solution, it can be prepared by using a conventional toothpaste solution. It can be prepared by mixing red bean flower extract and formulating it into a tooth-rinsing solution, and oral diseases can be prevented or treated by washing the mouth 2 to 10 times a day.

In addition, when the oral hygiene composition of the present invention is a toothpaste cream, it may additionally contain a liquid containing water and a moisturizer, a gelling agent or water-insoluble brightener, sweetener, flavoring agent, etc.

In addition, when the oral hygiene composition of the present invention is a mouthwash, it may further include a toothpaste carrier, more specifically, non-toxic alcohol.

The method for preparing the composition for treating or preventing oral diseases of the present invention can be prepared according to a method commonly used in the manufacturing industry.

The composition containing the red bean flower extract of the present invention exhibits excellent oral disease treatment or prevention effects.

The composition of the present invention can increase sallyberry mucin production.

The composition of the present invention can be used safely without concerns about resistance or resistance that may be caused by excessive use of antibacterial substances.

Figure 1 shows the mucin production rate when A-253 cells were treated with red bean flower extract at different concentrations, according to an embodiment of the present invention.

Hereinafter, to aid understanding of the present invention, it will be described in detail through examples. However, the embodiments according to the present invention may be modified into various other forms, and the scope of the present invention should not be construed as being limited to the following embodiments. Embodiments of the present invention are provided to more completely explain the present invention to those skilled in the art.

Example 1: Confirmation of cytotoxicity of red bean flower extract

To confirm the concentration of red bean flower extract that does not inhibit the cell viability of A-253, an oral salivary gland epithelial cell, the following cytotoxicity experiment was first performed. A-253 cells were inoculated into a 96-well microplate at a density of 1×10 ⁴ cells/well and cultured in a CO ₂ incubator at 37°C and 5% concentration for 24 hours. Next, the medium was removed and the cells were washed with Phosphate Buffered Saline (PBS), a washing buffer, and then washed with serum-free McCoy's 5A Medium, Modified (ATCC) medium. Red bean flower extract was added at different concentrations (0.02 ppm, 0.2 ppm, and 2 ppm) and cultured for 24 hours.

Cytotoxicity measurement was performed as follows using CCK-8 (Cell Counting Kit-8, DOJINDO Laboratories), which can observe the degree of cell proliferation. 10㎕ of CCK-8 solution was added to 96-well microplates treated with red bean flower extract at different concentrations (0.02 ppm, 0.2 ppm, and 2 ppm), and cultured in an incubator at 37°C and 5% CO ₂ for 1 hour and 30 minutes. Afterwards, the absorbance was measured at 450 nm using an ELISA reader (SPECTRA MAX 190, Molecular Devices). At this time, in order to determine whether the red bean flower extract was cytotoxic, each test was repeated three times and the average value was obtained. Cell proliferation rate was evaluated by the ratio of absorbance compared to the control group containing only McCoy's 5A medium and modified medium containing no red bean flower extract and no serum. Table 1 below shows the cytotoxicity results (cell proliferation rate) according to the concentration of red bean flower extract.

test substance Cell proliferation rate (%) control group 100 0.02 ppm red bean flower extract 100 0.2 ppm red bean flower extract 102 2 ppm red bean flower extract 101

As a result of the above experiment, even when the concentration of the red bean flower extract was increased to 2 ppm, there was no result of inhibiting cell proliferation, so it is believed that the cytotoxicity of the red bean flower extract is not a problem.

Example 2: Red bean flower extract sallybury mucin (salivary mucin ) Check the effect of promoting production

After treating A-253 epithelial cells with red bean flower extract, the effect of promoting sallyberry mucin production was measured. A-253 epithelial cells were inoculated into a 24-well microplate at a concentration of 2.0×10 ⁵ cells/well and cultured at 37°C in a 5% CO ₂ incubator for 24 hours. Next, the medium was removed, the cells were washed with phosphate-buffered saline as a washing buffer, and red bean flower extract was added to serum-free McCoy's 5A Medium, Modified medium at concentrations of 0.02 ppm, 0.2 ppm, and 2 ppm and incubated for 24 hours. cultured for a while.

Next, sallyberry mucin was analyzed using the supernatant. Salivary mucin was measured by purchasing a commercially available Human mucin ELISA kit (MyBioSource), and the measurement method was performed according to the instructions included in the kit. The sallyberry mucin production rate was evaluated by the ratio of absorbance compared to the control group containing only McCoy's 5A medium and modified medium containing no red bean flower extract and no serum. Table 2 and Figure 1 below show the celery mucin production rate according to the concentration of the red bean flower extract.

processing material Salivary mucin production rate (%) control group 100 Positive control group (IL-1β 10ng/ml) 125 0.02 ppm red bean flower extract 104 0.2 ppm red bean flower extract 119 2 ppm red bean flower extract 134

As a result of the above experiment, it was confirmed that the production of sallyberry mucin increased in a concentration-dependent manner when treated with red bean flower extract compared to the control group.

Specifically, A-253, an oral salivary gland epithelial cell, was treated with red bean flower extract at concentrations of 0.02 ppm, 0.2 ppm, and 2 ppm, and then the sallyberry mucin production rate was measured using a human mucin ELISA kit. As a result, the red bean flower extract was not treated. Compared to the control group, the sallyberry mucin production rate was approximately 1.04 to 1.34 times higher.

Considering the results of Examples 1 and 2, the treatment concentration of the red bean flower extract that does not show cytotoxicity to salivary gland epithelial cells in the oral cavity was confirmed from Example 1, and the red bean flower extract was treated as shown in Table 2 and Figure 1. It was found that the extract promotes the production of sallyberry mucin in saliva. This is a result that supports the fact that the red bean flower extract of the present invention is effective as a material that promotes the production of celery mucin in saliva.

Example 3: Confirmation of antibacterial effect of red bean flower extract

An experiment was conducted to confirm the antibacterial effect of red bean flower extract using Streptococcus mutans, a gram-positive bacterium, and Porphyromonas gingivalis, a gram-negative bacterium, which are representative bacteria that cause dental caries and periodontal disease. In order to confirm the inhibitory effect on the bacteria, an antibacterial test was performed using the paper disk test method. Each of the above bacteria was activated under the optimal culture conditions shown in Table 3 below, and then cultured in the optimal medium for each bacteria for about 4 to 6 hours to adjust the turbidity of the culture medium to Macfarland turbidity No. After adjusting to 0.5 (1.5x10 ⁸ ), 0.1 ml was spread evenly. A sterilized paper disk (Whatman no.5 paper, 8mm diameter) was inoculated with red bean flower extract at a concentration of 10 mg/disc, absorbed and dried for 1 hour, and then used. After culturing for 24-48 hours at the optimal temperature for each of the above bacteria, the size (diameter mm) of growth-inhibiting rings was measured. The results of measuring the antibacterial activity of the red bean flower extract against oral pathogens are shown in Table 4 below.

strain optimal conditions Gram stain
(Gram staining) Strain name temperature badge characteristics Gram (+) Streptococcus mutans 37℃ BHI Facultative anaerobic Gram (-) Porphyromonas gingivalis 37℃ TSA Hemin Menadione medium Anaerobic

Growth retardation ring diameter (mm) S. mutans P. gingivalis Untreated - (No inhibition) - (No inhibition) Red bean flower extract treatment 10.1 10.1

Example 4: Red bean flower extract gingival inflammation Confirmation of formation inhibition effect

In order to compare the degree of inhibition of gingival inflammation formation, first, sodium carboxymethyl cellulose, sodium lauryl sulfate, glycerin, colloidal silicon dioxide, silica, and sodium cocoyl isethionate, which can be commonly used to manufacture toothpaste. , a control toothpaste was prepared using dodicine and sweeteners, fragrances, and colorants. In addition, an experimental group toothpaste was prepared containing 0.01% by weight of red bean flower extract in the control group toothpaste.

The test subjects were gingival inflammation patients with even teeth and no missing teeth. A detailed oral examination was performed on 30 people by gender at 10-year intervals from 30 to 50 years old. Afterwards, 120 test subjects were selected, divided into 60 people, and a clinical experiment was conducted on the effectiveness of treating gingival inflammation.

The test subjects were divided into an experimental group and a control group and taught to use the product three times a day, two hours after a meal and before going to bed. Dental cleaning was performed on the experimental and control groups to score the initial gingivitis index. Afterwards, each toothpaste composition was used, and an oral examination was performed after 1 week, 1 month, 3 months, and 6 months to test the gingivitis index. The gingivitis index was measured by inserting a periodontal probe into the gingival sulcus, burning and probing around each tooth without applying force, and measuring the bleeding state after 30 seconds. The scores were recorded in the same manner as Table 5 below, and the results of measuring the effect of suppressing gingival inflammation are shown in Table 6 below.

score detail 0 points No bleeding state 1 point normal bleeding condition 2 points Linear hemorrhage condition 3 points Triangular bleeding condition in the interproximal area 4 points Gingival bleeding condition

hour control group test group 0 weeks 1.03 1.06 1 week 1.46 1.08 1 month 2.55 1.09 3 months 2.89 1.11 6 months 3.01 1.11

Example 5: Red bean flower extract Sirin Comparison of phenomenon suppression levels

In order to compare the degree of suppression of toothpaste, first, sodium carboxymethyl cellulose, sodium lauryl sulfate, glycerin, colloidal silicon dioxide, silica, sodium cocoyl isethionate, which can be commonly used to prepare toothpaste. A control toothpaste was made using dodicine and sweeteners, fragrances, and colorants. In addition, an experimental group toothpaste was prepared containing 0.01% by weight of red bean flower extract in the control group toothpaste.

The degree of inhibition of the cold tooth phenomenon of the toothpaste compositions prepared above was compared and measured. Specifically, 40 volunteers with hyperesthesia teeth who agreed to participate in this experiment were studied, and the total number of teeth studied was 80. Additionally, among the applicants, there were 20 men and 20 women, and their ages ranged from 20 to 50 years old. The study subjects were blinded to the contents of the toothpaste, and the total test period was 2 weeks.

The test was conducted by measuring the patient's response after applying a temperature stimulus. Before conducting the test, the hypersensitivity area of each applicant's hyperesthesia teeth was checked in advance. After evaluating the score by dropping about 5℃ cold water with a dropper on the sensitive area of the tooth, each composition is used 3 times a day for 2 weeks. After 2 weeks, about 5℃ cold water is dropped again with a dropper. The scores were evaluated. The patient's response rating standard after stimulation was set as follows (0 points: no discomfort, 1 point: slightly uncomfortable or sore, 3 points: pain). For statistical processing, the stimulation scores before and 2 weeks after the experiment were tested using a paired student-t test. The response scores (degree of cold inhibition) to temperature stimulation after 2 weeks are shown in Table 7 below.

hour control group experimental group 0 weeks 2.14±0.07 2.15±0.13 2 weeks 2.14±0.00 1.90±0.04 p>0.05 *p<0.05

Example 6: Experiment on the bad breath removal effect of red bean flower extract

To compare the degree of bad breath removal, first, sodium carboxymethylcellulose, sodium lauryl sulfate, glycerin, colloidal silicon dioxide, silica, sodium cocoyl isethionate, and dodicine, which are commonly used to manufacture toothpaste. A control toothpaste was prepared using sweeteners, fragrances, and colorants. In addition, an experimental group toothpaste was prepared containing 0.01% by weight of red bean flower extract in the control group toothpaste.

Fifty men and women without dental caries were selected and a cross-over test was conducted on the target drinks. Commercially available garlic powder was dispersed in water and left for 24 hours, then diluted to obtain a Halimeter measurement value of 700 ppb or higher, and the diluted solution was used as a bad breath inducer. After gargling with 15 ml of garlic powder dilution for 30 seconds, the degree of bad breath was measured with a halimeter 1 minute later. Afterwards, teeth were brushed for 30 seconds to 1 minute using the control or experimental group toothpaste. The degree of bad breath was measured with a halimeter 1 minute, 5 minutes, and 30 minutes after brushing teeth to determine whether the inhibition of bad breath lasted. The results of evaluating the effect of removing bad breath are shown in Table 8 below.

hour control group experimental group 1 min 56.2% 86.0% 5 minutes 39.9% 88.4% 30 minutes 37.9% 86.1%

From the above experiments, the composition of the present invention containing red bean flower extract as an active ingredient promotes the production of sallyberry mucin, which plays an important role as a barrier to protect the oral cavity from dry mouth and environmental stress, thereby preventing, improving or treating dry mouth. It was confirmed that it can be used as a material.

In addition, it was confirmed that the composition of the present invention can be used as a substance to effectively prevent, improve, or treat oral diseases caused by oral pathogens, such as dental caries, gingivitis, periodontitis, oral mucous membrane ulcers, bad breath, and dry teeth. did.

Claims (16)

A pharmaceutical composition for the prevention or treatment of one or more oral diseases selected from the group consisting of dental caries, bad breath, dry mouth, and dry mouth, containing an extract of red bean flower ( Daphne genkwa ) as an active ingredient. delete delete The pharmaceutical composition according to claim 1, wherein the oral disease is dry mouth. The pharmaceutical composition according to claim 1, wherein the red bean flower extract promotes the production of salivary mucin. An oral hygiene composition for preventing or improving one or more oral diseases selected from the group consisting of dental caries, bad breath, dry mouth, and dry mouth, containing red bean flower ( Daphne genkwa ) extract as an active ingredient. delete delete The oral hygiene composition according to claim 6, wherein the oral disease is xerostomia. The oral hygiene composition according to claim 6, wherein the red bean flower extract promotes the production of celery mucin. The oral hygiene composition according to claim 6, wherein the oral hygiene composition has one or more formulations selected from the group consisting of toothpaste, mouthwash, oral spray, oral ointment, and gum. A food composition for preventing or improving one or more oral diseases selected from the group consisting of dental caries, bad breath, dry mouth, and dry mouth, containing red bean flower ( Daphne genkwa ) extract as an active ingredient. delete delete The food composition according to claim 12, wherein the oral disease is dry mouth. The food composition according to claim 12, wherein the red bean flower extract promotes the production of celery mucin.