KR20100030453A - Cosmetic composition containing a pine bark extract - Google Patents

Abstract

PURPOSE: A functional cosmetic composition is provided to protect skin from irritative chemicals and external environment using pine bark extract containing proanthocyanidin(PA). CONSTITUTION: A functional cosmetic composition contains 0.5-3 weight% of pine bark extract containing proanthocyanidin, 5-20 weight% of butylenes glycol, 3-15 weight% of glycerin, 0.5-15 weight% of nano coenzyme Q10 and 1-3 weight% of arbutin, remaining amount of purified water. The functional cosmetic composition additionally contains ethanol, xanthan gum, sodium hyaluronate, and xyloglucan.

Description

Functional cosmetic composition using pine bark extract {Cosmetic composition containing a pine bark extract}

The present invention relates to a functional cosmetic composition for skin whitening and wrinkle improvement, more specifically, pine bark extract containing proanthocyanidins, purified water, butylene glycol, glycerin, nano-ized coenzyme Q10 and arbutin as components It relates to a functional cosmetic composition comprising a skin essence prepared using the same.

Proanthocyanidins, the main component of pine bark hydrothermal extract, are tannins present in various higher plants and have various biologically active functions. Proanthocyanidins are generally a group of compounds composed of two or more dimers by condensation or polymerization of flavan-3-ols and flavan-3,4-diols as structural units. Proanthocyanidins are a kind of plant polyphenols that have strong antioxidant power and are present in many plant leaves, fruit peels, and seeds.

Proanthocyanidins have been widely reported for astringent effects, anti-aging effects, anticancer effects, and antioxidant effects. Proanthocyanidins contained in plants can be obtained mainly by extraction. At this time, as a solvent used for extraction, an organic solvent such as water, methanol, ethanol, acetone, ethyl acetate or the like or a mixture thereof is used.

Coenzyme Q10 is a bioenergy and synonymous with natural antioxidants. Coenzyme Q10, a coenzyme that is classified as a vitamin or vitamin analog, is a fat-soluble substance that exists in nature and is present in almost every cell in the body. In particular, it contains a lot of organs such as heart, kidney, etc., and is present in large amount on the skin.

Coenzyme Q10 is known to decrease rapidly in various organs and heart with aging. Therefore, various health foods and medicines utilizing coenzyme Q10 are being developed to supplement the energy loss and fatigue caused by aging with the view of the concentration of coenzyme Q10. Coenzyme Q10 is an important coenzyme involved in the production of energy in the intracellular mitochondria, which helps to improve brain function and remove toxins. In addition, coenzyme Q10 is an essential nutrient found in all cells in our body, which plays a very important role in heart disease and anti-aging (long life), which is structurally similar to vitamin E and interferes with the activity of free radicals that cause illness in the body. It is a powerful antioxidant and is called a miracle vitamin. Studies have shown that so-called elderly diseases, including heart disease, heart attack, weakened immunity, and reduced vitality, are caused by a decrease in coenzyme Q10 in our bodies.

In skin, coenzyme Q10 begins to decline from the peak of twenties to about 70% by twenties. Coenzyme Q10 is known to be the first biological component that is caused by ultraviolet light and eliminates free radicals that cause skin aging. Therefore, cosmetics have been used as a main potency ingredient of antioxidant and anti-aging products for the purpose of removing the factors causing photo-aging. Recently, as an ingredient in external preparations, it has been found to be effective in inhibiting melanin production process, which is closely related to the elasticity and wrinkle reduction action as well as to improve the elasticity and wrinkles in vivo, and thus the number of cases applied to whitening cosmetics is increasing. In addition, it is also known to play a role in enhancing the antioxidant power by helping to regenerate various antioxidants in vivo, it is also used in vitamins.

Arbutin is a substance present in bareberry extract that inhibits the production of melanin in the skin and prevents the formation of blemishes and freckles.It enhances the skin whitening effect.It has been developed and used in Shiseido, Japan, and is the most used whitening agent worldwide Is one of. Arbutin is one of the whitening agents approved by the Korea Food and Drug Administration. Using arbutin with vitamin C can further enhance the whitening effect.

The whitening ingredients used in whitening cosmetics can be divided into two types according to their mechanism of action. One is vitamin C, which reduces the color of melanin that has already been produced. The other is to act directly on tyrosinase (an enzyme that oxidizes melanin in the presence of oxygen) to inhibit the production of melanin pigments, including arbutin and ellagic acid. Arbutin strongly inhibits the production of melanin by acting on the enzyme "tyrosinase," which produces melanin, which causes freckles and blemishes. Arbutin is also called hydroquinone by the sugar transfer reaction using an enzyme derived from a microorganism.

An object of the present invention is to use the functional cosmetic composition to help the skin whitening, improve wrinkles, and protect the skin exposed to undesirable external environment and irritant chemicals and the like.

To solve the above problems,

The present invention in the functional cosmetic composition for skin whitening and wrinkle improvement,

It provides a functional cosmetic composition comprising pine bark extract containing proanthocyanidins, purified water, butylene glycol, glycerin, nano-ized coenzyme Q10 and arbutin as a component.

The present invention also provides a skin essence prepared using the functional cosmetic composition.

The present invention is a functional cosmetic composition for skin beauty, causing the skin whitening effect, improve wrinkles, maintain proper moisture and elasticity to maintain healthy skin.

The present invention in the functional cosmetic composition for skin whitening and wrinkle improvement,

Consists of pine bark extract containing proanthocyanidin (PA), purified water, butylene glycol (BG), glycerin, nano-coenzyme Q10 and albutin It provides a functional cosmetic composition comprising as a component.

In the present invention, the component is 0.5 to 3% by weight of pine bark extract containing proanthocyanidins, 5 to 20% by weight of butylene glycol, 3 to 15% by weight of glycerin, 0.5 to 1.5% by weight of nanoenzyme Q10, Arbutin may be mixed with 1-3% by weight and the balance of purified water.

In addition, the functional cosmetic composition in the present invention is a pine bark extract containing proanthocyanidins, purified water, butylene glycol, glycerin, nano-ized coenzyme Q10 and arbutin other than ethanol, Xanthan gum, sodium hyaluronate (Sodium) hyaluronate, xyloglucan, and rose water may further comprise components.

In the present invention, the functional cosmetic composition is 0.5 to 3% by weight of pine bark extract containing proanthocyanidins, 5 to 20% by weight of butylene glycol, 3 to 15% by weight of glycerin, 0.5 to 1.5 weight of nano-ized coenzyme Q10 %, Arbutin 1-3 wt%, Ethanol 5-20 wt%, Xanthan gum 0.05-0.3 wt%, Sodium hyaluronate 0.001-0.1 wt%, Xylloglucan 0.005-0.02 wt%, Western rose water 7-13 wt% The remainder can be mixed with purified water.

The present invention includes a skin essence prepared using the functional cosmetic composition.

Hereinafter, the functional cosmetic composition for skin whitening and wrinkle improvement of the present invention will be described in detail.

The present invention uses pine bark extract containing proanthocyanidin as a main ingredient to lower the melanin and erythema index of the skin to have a whitening effect, and to maintain the moisture and elasticity of the skin to prevent wrinkles The composition relates to. Proanthocyanidin, a test material, is used as a constituent, and nano-enzyme Q10 and arbutin are included to prevent aging of the skin in addition to the above effects, and to have an effect on color stability of cosmetics.

In the present invention, the functional cosmetic composition is pine bark extract containing proanthocyanidins 0.5 to 3% by weight, butylene glycol 5 to 20% by weight, glycerin 3 to 15% by weight, nano-ized coenzyme Q10 0.5 to 1.5% by weight , Arbutin 1-3 wt% and the balance may be mixed with purified water.

Preferably, the functional cosmetic composition is 0.5 to 3% by weight of pine bark extract containing proanthocyanidins, 5 to 10% by weight of butylene glycol, 3 to 10% by weight of glycerin, nano-coenzyme Q10 0.5 to 1.5% by weight , Arbutin 1-3 wt% and the balance may be mixed with purified water.

More preferably, the functional cosmetic composition is 2% by weight of pine bark extract containing proanthocyanidins, 8% by weight of butylene glycol, 5% by weight of glycerin, 1% by weight of nanonized coenzyme Q10, 2% by weight of arbutin and glass It can be mixed with negative purified water. This is because the above ratio is a ratio that can exhibit the optimum effect as a cosmetic composition.

In the present invention, the functional cosmetic composition is a pine bark extract containing proanthocyanidins, purified water, butylene glycol, glycerin, nano-ized coenzyme Q10 and arbutin in addition to ethanol, xanthan gum, sodium hyaluronate, xyloglucan and western It may further comprise a component consisting of rose water.

In the present invention, the functional cosmetic composition is 0.5 to 3% by weight of pine bark extract containing proanthocyanidins, 5 to 20% by weight of butylene glycol, 3 to 15% by weight of glycerin, 0.5 to 1.5 weight of nano-ized coenzyme Q10 %, Arbutin 1-3 wt%, Ethanol 5-20 wt%, Xanthan gum 0.05-0.3 wt%, Sodium hyaluronate 0.001-0.1 wt%, Xylloglucan 0.005-0.02 wt%, Western rose water 7-13 wt% The remainder can be mixed with purified water.

Preferably, the functional cosmetic composition is 0.5 to 3% by weight of pine bark extract containing proanthocyanidins, 5 to 10% by weight of butylene glycol, 3 to 10% by weight of glycerin, nano-coenzyme Q10 0.5 to 1.5% by weight , Arbutin 1-3 wt%, Ethanol 5-10 wt%, Xanthan gum 0.2-0.3 wt%, Sodium hyaluronate 0.005-0.015 wt%, Xylloglucan 0.005-0.015 wt%, Western rose water 7-13 wt% It can be mixed with negative purified water.

More preferably, the functional cosmetic composition is 2% by weight of pine bark extract containing proanthocyanidins, 8% by weight of butylene glycol, 5% by weight of glycerin, 1% by weight of nanonized coenzyme Q10, 2% by weight of arbutin 8 It may be mixed with the weight percent, xanthan gum 0.25% by weight, sodium hyaluronate 0.01% by weight, xyloglucan 0.01% by weight, western rose water 10% by weight and the balance purified water.

The present invention includes a skin essence prepared using the functional cosmetic composition. The skin essence may include a thickener, a preservative, a transdermal absorbent or a surfactant in addition to the components included in the composition.

Hereinafter, an embodiment of the functional cosmetic composition of the present invention, an experimental example will be examined. However, the following Examples and Experimental Examples do not limit the scope of the present invention.

EXAMPLES Preparation of Test Materials (Preparation of Pine Bark Extract Containing Proanthocyanidins)

30 kg (dry weight) of radiata pine bark powder was used in the experiment to prepare a large amount of radiata pine extract known to have excellent physiological activity. The material used for the experiment produced two types as follows. That is, the radiata pine bark powder was extracted at 100 ° C. for 1 hour under the condition of liquid ratio 10. The extract was filtered through a 200 mesh filter and concentrated under reduced pressure below 50 ℃. The precipitate produced upon concentration was filtered off with a 10 μm rod cartridge. The filtrate was powdered by spray drying. Spray drying was performed under the conditions of 170 degreeC of discharge part, 90 degreeC of cyclone temperature, and 20,000 rpm. The prepared samples were vacuum dried in the presence of phosphorus pentoxide at room temperature for 24 hours to remove moisture and then sealed and stored. And the sample thus prepared is abbreviated as WS.

In order to lower the content of neutral sugar in the extract and to increase the content of the functional ingredient proanthocyanidin (PA) (see FIG. 1), a part of the filtered hot water extract is concentrated again to remove neutral sugars and the like. Anhydrous alcohol was treated. The resulting precipitate was filtered through a 10 μm rod cartridge, and concentrated under reduced pressure at 50 ° C., followed by vacuum drying at 60 ° C. using a hydrothermal circulation vacuum dryer. After vacuum drying, the sample was crushed using a high speed mill and used as a sample. The sample thus prepared was named ES.

 PA content of WS and ES was measured by butanol-HCl method, the content of each was 92.7% and 94.9%. Various tests were carried out using these samples as basic samples in cosmetic production.

Experimental Example 1 Appropriate Concentration of Pine Bark Extract

Pine bark extract containing proanthocyanidins for the distribution and storage of raw materials is preferably a powdery product, but it is difficult to mix and use immediately. Therefore, many cosmetic raw materials and additives are supplied and distributed in a certain concentration of liquid phase. High concentration of pine bark extract concentration in the mixed solvent system may be advantageous in terms of efficacy effect and handling, but the efficacy and effect of pine bark extract is sufficiently expressed in a small amount, so it is not necessary to prepare a high concentration of sample excessively. Therefore, as shown in Table 1, the WS and ES were adjusted to 0.5-3% by weight, respectively, and then formulated into the cosmetic formulation to determine the appropriate pine bark extract concentration as the color of the final product.

<Table 1> Appropriate concentration of pine bark extract (unit: weight%)

Prescription No. One 2 3 4 5 6 7 8  Purified water 49.5 49.0 48.0 47.0 49.5 49.0 48.0 47.0  Butylene Glycol (BG) 50.0 50.0 50.0 50.0 50.0 50.0 50.0 50.0  WS 0.5 1.0 2.0 3.0 - - -  ES 0.5 1.0 2.0 3.0 Sum 100.0 100.0 100.0 100.0 100.0 100.0 100.0 100.0

When the pine bark extract used in this experiment is dissolved in purified water and BG (butylene glycol) mixed solvent, it is dark reddish brown, which is generally avoided when manufacturing cosmetics. When manufacturing cosmetics using natural materials, such color problems are always raised. Therefore, when formulating them, the color needs to be adjusted so that the user does not feel rejection. At the same time, since the potency effect of the bark extract should be able to be expressed, such a strong color sample is difficult to color.

In the case of the sample concentration of 3% by weight or more, the concentration of the active ingredient is high, which is good in terms of efficacy effect, but the dark brown was strongly rejected when formulated. In case of less than 0.5% by weight, the color was pale pink, and the color was good, but it was necessary to use a large amount to fully exhibit the efficacy effect of the extract. In the case of 2% by weight, even when combined with the cosmetic formulation, it was a light brown color, which was not significantly different from commercially available natural plant cosmetics, and there was no great rejection. Therefore, in the subsequent cosmetic formulation test, Formula No. 2% by weight of pine bark extract concentrations of 3 and 7 (hereinafter referred to as 'WS-2') was used as a basic raw material.

Based on this formulation, the color stability of the extract was observed for 6 months, and the color gradually turned to darker dark brown color. Using a color difference meter, more accurate color change can be read. However, the phenolic compound mainly composed of PA polymerizes or decomposes due to light, and is gradually darkened and faded. It is a reality. In the case of PA, which maintains antioxidant activity for a long time in the air, it is no exception that cosmetic containers should be set so that light and air do not come into contact with each other in consideration of discoloration caused by light.

Experimental Example 2 Stability and Physiological Activity of Pine Bark Extract

1. Stability and Biological Activity Experiment

As shown in Table 2, the skin essence formulation was treated with 2% by weight of WS-2 and the stability was examined for 1 month at 5 ° C and 45 ° C. As a result, it was stable at 5 ° C but was poor at 45 ° C. This is because skin essence is due to oxidation because pine bark PA, an active ingredient, is in direct contact with air. Irradiation of ultraviolet light resulted in faster darkening of these formulations.

Table 2 Stability and Physiological Activity of WS-2 in the Formulation of Skin Essence

Skin essence WS-2, 2 wt% 5 Stability Room temperature, 1 day 5 45 ℃, one month 3 5 ℃, one month 4 Physiological activity Room temperature, 5 minutes 5 Room temperature, 2 hours One

Physiological activity, measured using DPPH (1,1-diphenyl-2-picrylhydrazyl), was also very low, with a similar trend to stability. This reason also seems to rapidly decrease its physiological activity by reacting with active oxygen in the air. Therefore, in order to suppress the discoloration and increase the antioxidant power during neglect, it was considered that it needs to be mixed with other antioxidants.

2. Addition experiment of bioactive substance

In experiments with various antioxidants, coenzyme Q10, a bioactive substance present in the mitochondria, and vitamin E (tocopherol), an antioxidant commonly known as a sample and cosmetic additive, delayed discoloration of WS-2 and enhanced antioxidant capacity. It was predicted that synergistic effects could be expected when it was found to be used in cosmetic formulations. Therefore, in anticipation of synergistic effect when used with WS-2, we tried to combine these antioxidants in formulation studies, functional cosmetic tests, and prototype manufacturing. However, since both raw materials are lipophilic, there are difficulties that are difficult to use in aqueous formulations such as skins, skin toners, essences and astringents. Soluble coenzyme Q10 and vitamin E were solubilized to an average particle size of about 30 nanometers in a transparent form, such as an aqueous solution, and applied to cosmetics with WS-2 (see FIG. 2).

Experimental Example 3 Formulation Research Experiment (Skin Essence)

1. Moisturizer Adjustment Experiment

Skin essence is a prescription made to combine the functions of skin and essence. Therefore, I tried to combine the function of non-sticky and fresh skin with the function of moist essence. First, the appropriate amount of moisturizer was examined. The conditions are shown in Table 7.

<Table 7> Moisturizer Adjustment Experiment (Unit: wt%)

               Prescription Material SE-1 SE-2 SE-3 SE-4 SE-5 SE-6 SE-7 One Purified water 80.0 75.0 70.0 75.0 70.0 70.0 65.0 Glycerine - 5.0 10.0 - - 5.0 5.0 Butylene Glycol (BG) - - - 5.0 10.0 5.0 10.0 Nano-coenzyme Q10 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Polysorbate 80 1.0 1.0 1.0 1.0 1.0 1.0 1.0 WS-2 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2 1% Carboxy Vinyl Polymer (Carbopol 941) 10.0 10.0 10.0 10.0 10.0 10.0 10.0 3 4% Sodium hydroxide 6.0 6.0 6.0 6.0 6.0 6.0 6.0 4 Preservatives q.s q.s q.s q.s q.s q.s q.s

Prescription SE-1 and SE-4 had a good first feel, but the afterfeel felt too dry. When reviewed for four seasons, prescription SE-3 and SE-7 showed excellent moisturizing power. However, Carboxy Vinyl Polymer (Carbopol 941 from Noveon, USA), a thickener used here, was used for the first touch, spreading, slippery, and sticky when absorbing the skin. .

2. Thickening Adjustment Experiment

The conditions for this experiment are shown in Table 8 below.

Table 8 Thickener Adjustment Experiment (Unit: wt%)

                Prescription Material SE-8 SE-9 SE-10 SE-11 SE-12 SE-13 SE-14 One Purified water 65.0 60.0 60.0 55.0 45.0 50.0 40.0 Glycerine 5.0 5.0 5.0 5.0 5.0 5.0 5.0 Butylene Glycol (BG) 10.0 10.0 10.0 10.0 10.0 5.0 10.0 Nano-coenzyme Q10 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Polysorbate 80 1.0 1.0 1.0 1.0 1.0 1.0 1.0 WS-2 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2 1% Carboxy Vinyl Polymer (Carbopol 941) 5.0 5.0 5.0 5.0 5.0 - - 1% CM-cellulose - 5.0 - - - - - 1% Xanthan gum - - 5.0 10.0 20.0 20.0 30.0 1% sodium hyaluronate 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1% Xyloglucan 1.0 1.0 1.0 1.0 1.0 1.0 1.0 3 4% Sodium hydroxide 3.0 3.0 3.0 3.0 3.0 - - 4 antiseptic q.s q.s q.s q.s q.s q.s q.s

As a result of the thickener test, the content of carboxy vinyl polymer was lowered to 5% by weight, and sodium hyaluronate and xyloglucan were added to adjust the test, but the feeling was not good. The addition of CM-cellulose had a smooth feel but poor absorption. The SE-11 and SE-12 containing xanthan gum were excellent, but the spread was a little bad and I felt too rich in my sense of smell. Therefore, carboxyvinyl polymer was excluded from the prescription, and the results of experiments on xanthan gum showed that the Xanthan gum content of prescription SE-13, 14 was about 20-30% by weight. As a result of excluding the carboxy vinyl polymer, there was an advantage of not having to use sodium hydroxide as a neutralizer.

3. Experiment with Arbutin and Western Rose Water Addition

In anticipation of synergy effect to enhance the whitening effect when using WS-2, arbutin was selected as a raw material and its content adjustment experiment was examined (Table 9).

Arbutin (albutin) has been used in Shiseido, Japan in 1989, and has been used all over the world. In Korea, it has been proved as a raw material for whitening cosmetics and has been used in many functional cosmetics. This whitening raw material does not reduce the melanin pigment already produced like vitamin C, but acts directly on tyrosinase and inhibits the production of melanin pigment.

TABLE 9 Arbutin and Western Rose Addition Experiment (Unit: wt%)

                Prescription Material SE-15 SE-16 SE-17 SE-18 SE-19 SE-20 SE-21 One Purified water 53.5 53.0 52.5 52.0 49.0 43.0 39.0 glycerin 5.0 5.0 5.0 5.0 5.0 5.0 5.0 BG 10.0 10.0 10.0 10.0 8.0 8.0 8.0 Nano-coenzyme Q10 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Polysorbate 80 1.0 1.0 1.0 1.0 1.0 1.0 1.0 WS-2 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Arbutin 0.5 1.0 1.5 2.0 2.0 2.0 2.0 2 1% Xanthan Gum 25.0 25.0 25.0 25.0 25.0 25.0 25.0 1% Sodium Hyaluronate 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1% xyloglucan 1.0 1.0 1.0 1.0 1.0 1.0 1.0 3 Western Roses - - - - 5.0 10.0 15.0 4 antiseptic q.s q.s q.s q.s q.s q.s q.s

Experimental results show that 1% by weight of arbutin added has a synergistic effect. When using 2% by weight arbutin was a bit stiff and stiff feeling, 2% by weight of arbutin was used for functional whitening products and BG, another raw material, was adjusted. In addition, natural rose scented rose water was added to the prescription to eliminate its own base odor, and there was a slight scent of rose rose to give freshness when used. Therefore, 10% by weight of the formulation SE-20 was considered to be the most appropriate. When using a general synthetic fragrance, a little more irritating than natural fragrance, and must use a surfactant, so that the skin irritation that can occur at this time to reduce the ease of manufacture was decided to use Western rose water (Table 9). In addition to Western rose water, it can be replaced with a water-soluble extract with good fragrance.

4. Ethanol Addition Experiment

Until now, only glycerin and BG were used as transdermal absorbents, and ethanol addition experiments were conducted to increase absorption (Table 10).

Table 10 Ethanol Addition Experiment (Unit: wt%)

                Prescription Material SE-22 SE-23 SE-24 SE-25 SE-26 SE-27 One Purified water 43.0 39.0 34.0 36.3 36.5 37 Ethanol 1.0 5.0 10.0 8.0 8.0 8.0 glycerin 5.0 5.0 5.0 5.0 5.0 5.0 BG 8.0 8.0 8.0 8.0 8.0 8.0 Nano-coenzyme Q10 1.0 1.0 1.0 1.0 1.0 1.0 Polysorbate 80 1.0 1.0 1.0 0.7 0.5 - WS-2 2.0 2.0 2.0 2.0 2.0 2.0 Arbutin 2.0 2.0 2.0 2.0 2.0 2.0 2 1% Xanthan Gum 25.0 25.0 25.0 25.0 25.0 25.0 1% Sodium Hyaluronate 1.0 1.0 1.0 1.0 1.0 1.0 1% xyloglucan 1.0 1.0 1.0 1.0 1.0 1.0 3 Western Roses 10.0 10.0 10.0 10.0 10.0 10.0 4 antiseptic q.s q.s q.s q.s q.s q.s

As a result, 5% by weight of ethanol was a little shorter, 10% by weight of alcohol was slightly strong, 8% by weight was the freshest, but the transdermal absorption was good. In addition, the experiment to reduce the content of polysorbate 80, a surfactant used here. As a result, prescription SE-27 without polysorbate 80 was able to reduce skin irritation due to the addition of ethanol, and the feeling was much better.

Accordingly, this essence prescription was developed based on SE-27 prescription, and the evaluation results are shown in Table 11.

<Table 11> Evaluation Results

Experimental prescription SE-14 SE-18 SE-20 SE-27 Evaluation category Stability 5 ℃ ◎ ◎ ◎ ◎ Very good: ◎ Good: ○ Normal: △ Bad: × 20 ℃ ◎ ◎ ◎ ◎ 45 ℃ ◎ △ ◎ ◎ Cycling ◎ ○ ◎ ◎ Color, dreadlocks △ △ ◎ ◎ First sense of smell ○ △ ○ ◎ Sticky × △ ○ ◎ Moist ○ ○ ○ ◎ Remarks Favorability: SE-27> SE-20> SE-14> SE-18

Experimental Example 5 Skin Application Test of Skin Essence of Experimental Example 4

As mentioned above, we developed skin essences through various experiments and examined the effects of these skin essences on women in their 40s and 60s (mainly 40s). The subjects of this study were 20 employees from Chonbuk National University and residents of Jeonju. The test was conducted on skin moisture, elasticity, melanin / erythema, wrinkles and burn observation to comprehensively evaluate them.

1. Skin oil

Lipid membranes of the skin derived from the sebaceous glands or the stratum corneum are essential for maintaining skin physiology, but too much oil is associated with acne and seborrheic dermatitis. Therefore, accurate measurement of this oil is a great help in determining the skin condition. Sebumeter is an instrument that measures the amount of sebum that comes out after attaching a special translucent lipid absorption tape to the skin. Table 12 shows the results of measuring the amount of oil after washing 20 subjects and keeping them in a constant temperature and humidity room for 30 minutes. In this experiment, the temple or the lower part of the eye was measured to measure oil. Most of the subject's oil content was very low, below 10, which was only 1/10 of 40-90, which is not shown here, such as cheeks and temples. The reason for this is that the subjects washed their face before the measurement of oil and only 30 minutes after face washing did not supply enough oil.

<Table 12> Oil content of each subject measured in Sebumeter (unit: ㎍ / cm)

Subject Oil 0 1 week 2 weeks 3 weeks 4 Weeks 5 Weeks 6 Weeks Week 7 JS 2 One 2 2 2 One 2 2 2. JE 4 8 One 10 7 6 11 12 3. YZ 4 2 2 One One 2 2 One 4. NS 2 One 3 One One One One 2 5. KS One One One One 5 One One One 6. MR 5 One 3 2 4 2 8 9 7. KR One One 2 3 2 One One 4 8. MS 3 One One 2 One One One One 9. YS One 2 One One 2 3 One 2 10. JR 4 2 2 One 6 21 11 2 11. HS 6 2 3 13 6 9 4 4 12.YH 3 2 3 One 5 22 2 One 13. HA One 7 2 5 2 4 One 6 14. SJ 11 27 6 10 8 6 22 21 15.YR 6 3 7 6 10 One 5 9 16.OS 2 6 5 3 4 5 3 10 17. HS 5 5 3 3 2 3 2 18 18.SN 2 One One One 16 2 One One 19.JH 4 2 5 5 6 6 3 8 20.YM 5 8 16 9 21 7 27 9 Average 4 4 3 4 6 5 5 6

Table 13 below shows the degree of moisturizing of each subject measured by a corneometer. Skin moisturization is achieved by measuring the electrostatic load capacity of a slight current delivered through a probe in contact with the skin surface. If the skin's moisturizing degree is high, this value is increased, so that the skin's wet condition can be easily and quickly detected. Most of the subjects had a moisturizing level of about 50-70, and this value is an appropriate skin wetting state, as judged from the reference data shown in Table 14 below. Although some subjects had very high moisturizing levels, most of the subjects were found to return to proper wet conditions in the 30 minutes after washing the face, unlike the oil.

<Table 13> Moisturization of Skin by Corneoometer (Unit: AU)

Subject Moisturizing 0 1 week 2 weeks 3 weeks 4 Weeks 5 Weeks 6 Weeks Week 7 JS 55 61 64 63 65 61 60 67 2. JE 59 62 75 69 71 65 72 68 3. YZ 60 57 57 55 52 63 62 59 4. NS 65 65 63 57 56 61 64 66 5. KS 57 59 65 62 60 61 62 62 6. MR 56 52 60 60 63 62 60 57 7. MS 74 57 62 60 66 66 70 61 8. US 70 68 72 71 73 70 63 84 9. YS 60 58 59 59 68 70 52 63 10. JR 55 60 56 58 69 73 73 62 11. HS 52 57 46 63 68 46 51 59 12.YH 58 54 47 53 67 70 67 54 13. HA 49 62 58 54 55 57 48 60 14. SJ 55 47 58 61 66 69 62 61 15.YR 73 69 67 71 68 72 76 83 16.OS 51 64 71 64 73 66 63 65 17. HS 71 73 66 68 82 73 76 86 18.SN 64 63 66 64 70 68 67 64 19.JH 65 60 62 62 63 65 63 71 20.YM 74 79 80 76 81 80 78 75 Average 61 61 63 63 67 66 64 66

<Table 14> Reference data for interpretation of Coneometer measurement results (Unit: AU)

Measurement site type Forehead, T-zone, Hair, Cheek, Eyelid, Temple, Mouth Edge, Upper Body, Back, Neck Arms, hands, legs, elbows Very dry <30 <15 dry 30-49 15-29 Moisturizing 50-59 30-39 Moisturizing enough > 60 > 40

2. skin elasticity

A negative pressure is generated inside the equipment of the cutometer, the skin is sucked into a small hole in the probe, and the skin tries to return to its original form. This device uses the characteristics of the skin. The elasticity value is most ideal when the value in Table 15 is close to 1, and the network of collagen and elastin constituting the dermal layer is well formed.

Increasing the elasticity of the skin indirectly means that the components constituting the skin, ie, skin elements such as collagen and elastin, proliferate or increase resistance from collagenase, reactive reactive oxygen species, and the like. Although the experiment was never long for 8 weeks from the first measurement date, the average elasticity of the subjects tended to increase from 4 weeks after the test.

<Table 15> Measurement results of skin elasticity by cutometer (unit: AU)

 Subject Skin elasticity 0 1 week 2 weeks 3 weeks 4 Weeks 5 Weeks 6 Weeks Week 7 JS 0.56 0.53 0.68 0.62 0.65 0.60 0.61 0.61 2. JE 0.49 0.48 0.55 0.62 0.52 0.572 0.62 0.76 3. YZ 0.60 0.61 0.52 0.48 0.64 0.45 0.54 0.57 4. NS 0.43 0.52 0.64 0.53 0.57 0.52 0.55 0.62 5. KS 0.57 0.55 0.54 0.56 0.53 0.55 0.56 0.52 6. MR 0.53 0.38 0.42 0.45 0.49 0.51 0.50 0.49 7. KR 0.65 0.67 0.57 0.66 0.58 0.65 0.62 0.61 8. MS 0.63 0.52 0.61 0.54 0.45 0.55 0.58 0.58 9. YS 0.55 0.57 0.65 0.58 0.59 0.55 0.58 0.63 10. JR 0.63 0.63 0.53 0.59 0.48 0.62 0.63 0.46 11. HS 0.55 0.41 0.60 0.60 0.56 0.53 0.52 0.66 12.YH 0.65 0.54 0.50 0.58 0.63 0.58 0.48 0.55 13. HA 0.76 0.71 0.64 0.66 0.65 0.65 0.66 0.65 14. SJ 0.57 0.58 0.53 0.48 0.48 0.57 0.61 0.57 15.YR 0.59 0.52 0.60 0.54 0.60 0.57 0.54 0.64 16.OS 0.46 0.51 0.57 0.48 0.51 0.48 0.62 0.50 17. HS 0.54 0.55 0.46 0.51 0.46 0.58 0.62 0.59 18.SN 0.56 0.52 0.41 0.45 0.54 0.52 0.53 0.57 19.JH 0.58 0.35 0.48 0.50 0.49 0.38 0.42 0.53 20.YM 0.62 0.57 0.65 0.66 0.58 0.62 0.73 0.62 Average 0.58 0.54 0.56 0.55 0.55 0.55 0.58 0.59

3. Melanin / erythema

Skin tone is determined by the content and distribution of melanin and hemoglobin. Mexameter has 16 sensor diodes with three different wavelength bands for melanin and hemoglobin that are most suitable for skin tone analysis. It is very advantageous to determine the effect according to.

In this study, we used this probe to determine the whitening effect of cosmetics. The measured results are shown in Table 16. For Asians, as shown in the classification table (Table 17), the melanin levels of most subjects of Asian / Indian type were lower than Asian / Indian type of European and Mediterranean type. Some of the 7, 9, 10, 11, 13, and 20 had severe pigmentation sites and showed unusually high values. The results were examined with particular care to confirm the whitening effect of the present cosmetics.

<Table 16> Melanie / erythema analysis results measured by Mexameter (unit: AU)

Subject Melanin / erythema measurements 0 1 week 2 weeks  3 weeks 4 Weeks 5 Weeks 6 Weeks Week 7 JS 103 100 111 101 101 99 110 107 2. JE 121 138 114 90 91 115 118 110 3. YZ 119 151 121 116 81 110 122 117 4. NS 128 147 116 141 97 119 121 104 5. KS 147 184 158 159 153 159 132 143 6. MR 160 164 162 156 135 140 154 137 7. KR 251 167 137 137 125 123 144 141 8. MS 113 95 93 106 102 99 92 94 9. YS 269 340 268 267 252 251 242 241 10. JR 202 202 176 162 165 165 158 184 11. HS 232 200 207 235 180 166 199 185 12.YH 182 167 165 100 90 74 94 99 13. HA 212 179 138 122 129 136 131 120 14. SJ 199 128 91 74 57 72 77 73 15.YR 153 139 123 126 123 137 131 114 16.OS 187 188 184 186 176 174 169 159 17. HS 154 132 104 103 130 128 114 100 18.SN 173 182 162 165 178 144 164 161 19.JH 198 274 212 201 193 152 160 153 20.YM 256 240 227 235 229 219 218 198 Average 178 176 153 149 139 139 142 137

<Table 17> Reference data on race classification to interpret skin tone measurement results by Mexameter (unit: AU)

type Classification Average melanin content I Celtic type 0-49 II Caucasian 50-99 III European mixed type 100-149 IV Mediterranean 150-199 V Asian / Indian 200-299 VI Black skin 300-999

Melanin / erythema levels dropped sharply to about 4 weeks as shown in FIG. 3, after which there was no significant change. It was thought that the whitening effect was highest in the 4-week trial because the skin barrier was almost completely replaced in about 4 weeks.

Subjects with severe pigmentation showed burn results before and after 8 weeks of cosmetic testing (see FIGS. 4 and 5). As shown in the picture, many of the pigmentation sites in the same area disappeared, and the overall brightening can be seen. That is, it was confirmed that this tense cosmetics had the outstanding whitening effect. This was thought to be an effect of arbutin, but pine bark PA was thought to increase the effect of attenuating melanoma cells to prevent melanin metastasis.

4.wrinkle improvement effect

The skin surface image analysis measurement technique is indispensable for directly observing skin changes, but a general optical image magnification apparatus has been used since the past. Changes in the skin observed only with magnified images using a conventional halogen light source are not only different from the actual measurement conditions, but there are limitations on accurate measurement of skin wrinkles.In the actual test conditions, profilometry using replicas was used. Useful methods and even laser profilometry take a long time to process a single sample. Therefore, Skin Visiometer, a measurement technique that can process many replica samples in a short time, was used in this study.

This device features a standard cartridge designed to pass a special light source, inserts a wrinkled skin form obtained from a blue silicone replica, runs a program, and selects a specific area on the screen to objectively evaluate the depth and shape of the wrinkles. have.

In this study, skin roughness measurement mode was selected among various measurement modes of this instrument, and R5 value was calculated from R1 to examine skin condition. That is, as mentioned in the experimental method, R1 represents skin roughness and is a value representing the difference between the highest peak value and the lowest bone value in the selected image. R2 is the highest value of R1 of the image pieces divided into five equal lengths with average roughness. R3 represents the maximum roughness and is an average value of the R1 values of the respective image pieces divided into five equal parts. R4 is the difference between the maximum peak value and the center line, forming a centerline when the line is drawn to the highest peak at a smooth depth. Finally, R5 is the arithmetic mean roughness which is the central standard deviation of the reference profile for R4. The measured parameters can be integrated with the energy, variance and contrast index of the overall skin texture.

Table 18 below shows the results for 7 weeks for R1-R5 values for 6 subjects.

Table 18. Skin wrinkle results after 0 and 7 weeks as measured by Skin Visiometer

Subject wrinkle 0 Week 7 R1 R2 R3 R4 R5 R1 R2 R3 R4 R5 1. YS 74.4 64.02 37.7 22.3 8.9 77.9 59.6 38.7 23.9 10.1 2. KR 79.0 57.4 39.1 30.7 13.3 70.4 61.8 36.0 18.4 6.2 3. YH 98.4 85.1 56.4 27.1 13.8 65.2 51.3 34.1 23.0 9.7 4. JE 86.2 58.5 36.6 37.5 13.4 62.0 50.2 33.6 20.4 8.4 5. HA 69.2 55.6 33.9 21.3 9.6 73.6 61.2 39.0 22.8 9.2 6. NS 69.1 46.1 28.8 27.5 12.9 61.8 48.8 28.2 19.6 9.1 Average 68.0 53.8 38.8 23.8 10.7 68.5 47.7 30.7 18.8 8.1

The mean values for the initial start and post-week 7 results for six subjects decreased for each corresponding R1-R5 value.

That is, the present cosmetic was thought to have an anti wrinkle effect by 7 weeks of treatment. However, it was judged that it was difficult to fully explain the wrinkle effect as a result, and the results of five new subjects were measured again for 2 weeks and 7 weeks, and are shown in Table 19 below.

Table 19. Skin roughness after 2 and 7 weeks as measured by Skin Visiometer

Subject wrinkle 2 weeks Week 7 R1 R2 R3 R4 R5 R1 R2 R3 R4 R5 7. MS 93.6 60.9 40.2 39.9 16.8 74.2 64.6 42.7 24.3 8.9 8. OS 69.0 46.9 33.3 32.2 12.3 64.9 54.3 29.8 21.8 7.6 9. HS 66.0 57.0 35.2 16.7 7.7 56.2 47.6 31.9 17.4 7.4 10.SN 99.9 84.2 60.7 41.3 15.1 70.0 59.4 40.2 24.7 10.0 11.YM 85.6 71.7 46.5 30.2 10.8 57.6 47.9 31.4 19.4 7.8 Average 65.9 50.2 34.5 32.1 11.4 53.3 45.6 29.5 18.4 7.8

Comparing the R1-R5 values after 2 weeks and 7 weeks, the results showed that each value was very low after 7 weeks than 2 weeks. 6 shows a bar graph for easier comparison of Table 19.

The present invention satisfies the needs of consumers using the same by providing a cosmetic composition having a higher quality and functionality, from the supplier's point of view to profit from industrialization through mass production, and ultimately contribute to the industrial development of the country It is expected to play a big role.

Figure 1 shows the structural formula of proanthocyanidin (PA) which is the main component of the radiata pine bark extract.

2 shows Nano-Coenzyme Q10.

Figure 3 shows the change over time of melanin / erythema.

4 and 5 are photographs showing the experimental results before the cosmetic test (Fig. 4) and 8 weeks after the test (Fig. 5) for the subjects with severe pigmentation.

Figure 6 shows the result of comparing the skin roughness after 2 weeks and 7 weeks with a bar graph to compare Table 19 more easily.

Claims (5)

In the functional cosmetic composition for skin whitening and wrinkle improvement, A functional cosmetic composition comprising pine bark extract containing proanthocyanidins, purified water, butylene glycol, glycerin, nanonized coenzyme Q10 and arbutin as components. According to claim 1, wherein the composition is 0.5 to 3% by weight of pine bark extract containing proanthocyanidins, 5 to 20% by weight of butylene glycol, 3 to 15% by weight of glycerin, nano-ized coenzyme Q10 0.5 to 1.5 Functional cosmetic composition, characterized in that mixed by weight%, arbutin 1-3% by weight and the balance of purified water. The functional cosmetic composition of claim 1, wherein the functional cosmetic composition comprises ethanol, xanthan gum, sodium hyaluronate, xyloglucan, in addition to pine bark extract containing proanthocyanidins, purified water, butylene glycol, glycerin, nano-ized coenzyme Q10 and arbutin. And functional cosmetic composition, characterized in that it further comprises a component consisting of Western rose water. According to claim 3, The functional cosmetic composition is pine bark extract containing proanthocyanidins 0.5 to 3%, butylene glycol 5 to 20% by weight, glycerin 3 to 15% by weight, nano-ized coenzyme Q10 0.5 ~ 1.5 wt%, arbutin 1-3 wt%, ethanol 5-20 wt%, xanthan gum 0.05-0.3 wt%, sodium hyaluronate 0.001-0.1 wt%, xyloglucan 0.005-0.02 wt%, western rose water 7-13 wt% Functional cosmetic composition, characterized in that the mixture in% and the balance of purified water. Skin essence prepared using the functional cosmetic composition of any one of claims 1 to 4.

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