KR20090055957A - Cosmetic composition for skin moisturizing comprising the extract of orostachys japonicus as active ingredient - Google Patents

Abstract

A cosmetic composition containing Orostachys japonicus extract for skin moisturizing is provided to promote keratinocyte differentiation, recover skin barrier function and improve skin elasticity. A cosmetic composition for promoting keratinocyte differentiation, recovering skin barrier function, and moisturizing a skin comprises 0.001-30.0% by weight of Orostachys japonicus extract. The Orostachys japonicas extract is obtained through a solvent extraction method using water, hydrous or anhydrous low alcohol of 1-4 carbon atoms, acetone, ethyl acetate, chloroform, butylacetate or 1,3-butylene glycol, supercritical extraction or ultrasonication extraction. The cosmetic composition further comprises antioxidant, solubilizer, vitamin, pigment, and carrier. The cosmetic composition is used in a form of skin, lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray, or powder.

Description

Cosmetic Composition for Skin Moisturizing Comprising the Extract of Orostachys japonicus as Active Ingredient}

An object of the present invention relates to a cosmetic composition for moisturizing skin containing Waksong extract as an active ingredient, and more particularly, containing Waksong extract as an active ingredient, which is effective in promoting skin moisturizing, promoting skin keratinocyte differentiation and restoring skin barrier function. It relates to a cosmetic composition.

The skin is divided into three layers of epidermis, dermis and subcutaneous tissue in order from the outside, and it protects the organs of the body from temperature and humidity changes, ultraviolet rays and other physical and chemical external stimuli. In particular, the epidermis plays an important role in preventing moisture evaporation inside the human body. The epidermis is divided into the stratum corneum, granule layer, pore layer, and basal layer in order from the outside, and the cells of the stratum corneum act as bricks, and the intercellular lipids between the stratum corneum act as the mortar to form the skin barrier ( J. Invest.Drmatol. 80 (Suppl.), 44-49. 1983. In addition, keratinocytes of healthy people have high concentrations of Natural Moisturing Factor (NMF) to help the skin retain moisture. For example, because amino acids such as amino acids are water-soluble, they effectively combine with moisture Inhibit drying (J. Invest. Dermatol. 54, 24-31, 1970).

However, these days, the artificial temperature control of air conditioning and heating according to the change of environment and living patterns, the skin stress caused by various stresses and environmental pollution that occur in social life, the frequent face wash and the age increase due to makeup habits Due to various causes such as aging of the skin, the moisture of the stratum corneum decreases, the skin becomes dry, the surface becomes rough, and the skin becomes crumbly and loses luster, and the appearance of dullness occurs. Conventionally, a method of increasing moisture retention in the stratum corneum by using a humectant having a property of absorbing moisture or an occlusive moisturizer that prevents evaporation of water as a moisturizing agent is used. Examples of the humectants include glycerin, propylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, and 2-pyrrolidone-5-sodium carbonate. There is a downside to this feeling. In addition, as a closed moisturizer, lipid components such as ceramide, essential fatty acids and lipid complexes were used (J. Invest. Dermatol. (5), 731-740, 1994). There are disadvantages inadequate for the manufacture of the tops.

However, in recent years, the claim that differentiation of the stratum corneum is important to prevent drying of the skin moisture, the differentiation process that develops from the basal keratinocytes to the outermost stratum corneum cells must be performed normally to act as a barrier to skin moisture retention. It turns out that you can. That is, during the process of keratinization, the cells produce natural moisturizing factors and intercellular lipids, and as a result, the stratum corneum becomes firm and flexible, thus functioning as a barrier. Physiologically, the tendency of the skin to dry with age increases, which can be interpreted as increasing the dropout time of the stratum corneum, decreasing the lipid synthesis ability of epidermal cells, and decreasing the amount of moisturizing factors and lipids in the stratum corneum. . Thus, by accelerating the differentiation of keratinocytes, a new approach has been made that can induce a strengthening of the skin barrier and enhance skin moisture retention and protection from the external environment.

Accordingly, the present inventors studied the cosmetic composition excellent in the moisturizing effect of the skin to improve the skin pulling, roughness, lack of gloss, etc., but not sticky or sloppy when applied to the skin, and excellent formulation stability, containing the pine extract It was confirmed that the skin moisturizing effect was actually excellent when applied to human skin, and this moisturizing effect was achieved by promoting the differentiation of keratinocytes and inducing the strengthening of the skin barrier to improve skin moisture retention function. It was found that the present invention was completed.

Accordingly, it is an object of the present invention to provide a cosmetic composition for promoting the differentiation of keratinocytes of the skin, which contains the vortex extract as an active ingredient.

Still another object of the present invention is to provide a cosmetic composition for restoring skin barrier function, which contains the vortex extract as an active ingredient.

Another object of the present invention to provide a cosmetic composition for moisturizing the skin containing the extract as an active ingredient.

Another object of the present invention is to provide a cosmetic method comprising applying a cosmetic composition containing the vortex extract to human skin.

Other objects and advantages of the present invention will become apparent from the following detailed description and claims.

The present invention relates to a cosmetic composition for skin moisturizing containing the pine fruit extract as an active ingredient, and more particularly, containing the pine fruit extract as an active ingredient, in addition to the skin moisturizing effect, promoting the differentiation of keratinocytes of the skin and restoring skin barrier function. It relates to a cosmetic composition. The cosmetic composition according to the present invention contains the vortex extract as an active ingredient, promotes the differentiation of keratinocytes of the skin and restores the skin barrier function, relieves skin pulling, roughness and increases elasticity. It can be usefully used as.

The present invention relates to a cosmetic composition for moisturizing the skin containing the hawthorn extract as an active ingredient, and more particularly, by containing the hawsong extract as an active ingredient, it is effective in moisturizing skin, inducing the differentiation of skin keratinocytes, and enhancing skin barrier function. It relates to a cosmetic composition.

Orostachys japonicus included in the cosmetic composition of the present invention is a perennial plant with the dicotyledonous cultivars Barphyllaceae and grows in the field or on the roadside. 20-50 cm in height, without hairs, stems straight in red mauve round barrel. Lots of branches and roots at nodes. Leaves alternate, 4-8 cm long. Flowers bloom in red or purple or white in June-September, forming a 1-5 cm long inflorescence at the end of branches. When the leaves and stems are removed and shed on the stream, the fish are stunned because of their toxicity.

As used herein, the term “wasson extract” refers to those obtained by extraction from various organs or parts of the rosette (eg, leaves, flowers, roots, stems, branches, bark and seeds, etc.), preferably leaves, stems, branches , Bark or root, more preferably leaves, branches or roots, most preferably extracts obtained from leaves.

The Warsong extract of the present invention may be prepared according to conventional methods known in the art, i.e., under conditions of conventional temperature and pressure, using conventional solvents.

As the extract of the present invention, at least one extractant selected from the group consisting of purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane, chloroform, ethyl ether, butylene glycol and hexane Extraction by using, preferably ethanol, 70% ethanol or water is extracted, more preferably using 70% ethanol is characterized in that the extraction.

On the other hand, it is apparent to those skilled in the art that the vortex extract of the present invention can obtain an extract having substantially the same effect using not only the aforementioned extraction solvent but also other extraction solvents.

In addition, the extract of the present invention includes not only the extract by the above-described extraction solvent, but also the extract that has undergone a conventional purification process. For example, decompression by carbon dioxide, extraction by supercritical extraction by high temperature, extraction by ultrasonic extraction, separation using ultrafiltration membranes with constant molecular weight cut-off values, various chromatography (size, charge, hydrophobicity or affinity) The active fraction obtained through various purification methods additionally carried out, such as separation by sex)) is also included in the extract of the present invention.

In another aspect, the present invention provides a composition for external application for skin, characterized in that the extract is obtained by cold condensation at room temperature, heat filtration, and further obtained by concentrating the solvent under reduced pressure or lyophilization.

In addition, the Wasong extract of the present invention is 0.001-30.0% by weight based on the entire cosmetic composition, preferably characterized in that it contains 0.1-10.0% by weight. If the content of the extract is less than 0.001% by weight does not appear to improve the skin, when more than 30.0% by weight of the effect of increasing the effect of the content is insignificant, there is a problem in the safety and stability of the formulation and economical.

According to the present invention, the vortex extract is excellent in promoting keratinocyte differentiation (Experimental Example 1), skin barrier function restoring effect (Experimental Example 2) and moisturizing effect (Experimental Examples 3 and 4).

Therefore, the cosmetic composition of the present invention comprising such an extract as an active ingredient has an excellent moisturizing effect.

Ingredients included in the cosmetic composition of the present invention as an active ingredient includes components conventionally used in cosmetic compositions in addition to the active ingredient, for example, conventional auxiliaries such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavors, And carriers.

The cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing , Oils, powder foundations, emulsion foundations, wax foundations and sprays, and the like, but are not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, and microcrystals are used as carrier components. Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

In addition, the present invention provides a cosmetic method characterized in that the cosmetic composition containing the walrus extract of the present invention is applied to human skin.

The cosmetic method of the present invention refers to all cosmetic methods for applying the cosmetic composition of the present invention to human skin. That is, all the methods known in the art for applying the cosmetic composition to the skin belong to the cosmetic method of the present invention.

The cosmetic composition of the present invention may be used alone or in duplicate, or may be used in combination with other cosmetic compositions other than the present invention. In addition, the cosmetic composition with excellent skin protection effect according to the present invention can be used according to a conventional method of use, the number of times of use can be varied according to the user's skin condition or taste.

When the cosmetic composition of the present invention is a soap, surfactant-containing cleansing or surfactant-free cleansing formulation, it may be wiped off, peeled off or washed with water after application to the skin. As a specific example, the soap is liquid soap, powdered soap, solid soap and oil soap, the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, cleansing towels and cleansing pack, the surfactant-free cleansing formulation is a cleansing cream , Cleansing lotion, cleansing water and cleansing gel, but are not limited thereto.

When performing a cosmetic method comprising applying the cosmetic composition comprising the Wahsong extract of the present invention to the human skin, it is possible to obtain a skin keratinocyte differentiation promoting effect, skin barrier function recovery effect and skin moisturizing effect.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention. .

Preparation Example 1 Preparation of Wasong Extract

The dried and dried leaves of the dry pine was refluxed three times for 5 hours with a 70% (V / V) ethanol aqueous solution, and then cooled and filtered through Whatman # 5 filter paper. The filtered extract was concentrated under reduced pressure and lyophilized at 50 ° C or lower. The vacuum concentrate and the lyophilisate were dispersed / dissolved in 50% butylene glycol solution to prepare a vortex extract to be 1%.

Experimental Example 1. Keratinocyte differentiation promoting effect

Human keratinocytes were placed in a culture flask, cultured, and grown to about 80%. Then, the Wasong extract was added at a concentration of 10, 50, 100, or 200 ppm as shown in Table 1, followed by 4 days of treatment, followed by urea. Or a denaturant such as sodium dodecyl sulfate (SDS) and a reducing agent such as β-mercaptoethanol were mixed to remove the dissolved protein. Then, the peptide concentration was analyzed by measuring the absorbance at 310 nm of the remaining stratum corneum, and the increase by each sample was compared based on distilled water as a negative control (0%).

Concentration (ppm) Keratinocyte differentiation promoting effect (%) 10 36 50 55 100 61 200 80 Negative Control (Distilled Water) 0

As shown in the results of Table 1, it was confirmed that the vortex extract used in the present invention is very excellent in promoting the differentiation of keratinocytes.

Prescription 1: Softener

Formulation examples of the flexible cosmetics in the cosmetic containing the Wahsong extract of Preparation Example 1 are shown in Table 2.

ingredient Content (unit: weight%) Warson Extract Glycerin 1.3-Butylene Glycol Fiji 1500 Allantoin DL-Pantenol E.D.T.A-2NA Benzophenone-9 Sodium Hyaluronate Ethanol Octicdodeceth-16 Polysorbate 20 Preservative, Flavor, Pigment Distilled Water 1.0 5.0 3.0 1.0 0.1 0.3 0.02 0.04 5.0 10.0 0.2 0.2 Sum 100

Prescription Example  2: convergence makeup

Prescription examples of astringent cosmetic water in the cosmetic composition containing the Wasong extract of Preparation Example 1 are shown in Table 3 below.

ingredient Content (unit: weight%) Edison Extract Glycerin 1.3-Butylene Glycol Allantoin DL-Pantenol EDTA-2Na Benzophenone-9 Sodium Hyaluronate Ethanol Polysorbate 20 Witch Hazel Extract Citric Acid Preservative, Flavor, Pigment Distilled Water 1.0 2.0 2.0 0.2 0.2 0.02 0.04 3.0 15.0 0.3 2.0 Sum 100

Prescription Example  3: nutrition cosmetics

Prescription example of nutritional longevity among cosmetics containing Waksong extract of Preparation Example 1 is shown in Table 4.

ingredient Content (unit: weight%) Warson Extract Glyceryl Stearate SE Stearyl Alcohol Lanolin Polysorbate 60 Solbitan Stearate Cured Vegetable Oil Mineral Oil Squalane Trioctanoine Dimethicone Tocopherol Carboxyvinyl Polymer Glycerin 1.3-Butylene Glycol Sodium Hyaluronate Tri Ethanolamine Preservative Pigmented distilled water 2.0 1.5 1.5 1.5 1.3 0.5 1.0 5.0 3.0 2.0 0.8 0.5 0.12 5.0 3.0 5.0 0.12 Sum 100

Prescription Example  4: nutrition cream

Formulation example of the nutrition cream in the cosmetic containing Waksong extract of Preparation Example 1 is shown in Table 5.

Component (content: weight%) Example 1 Comparative Example 1 Edible Extract Lipophilic Monostearate Glycerin Stearic Acid Stearic Acid Beeswax Polysorbate 60 Sorbitan Stearate Cured Vegetable Oil Squalane Mineral Oil Trioctanoin Dimethicone Sodium Magnesium Silicate Glycerin Betaine Triethanolamine Sodium Hyaluronate Preservative, Flavor, Color Distilled Water 3.0 2.0 2.2 1.5 1.0 1.5 0.6 1.0 3.0 5.0 5.0 1.0 0.1 5.0 3.0 1.0 4.0 -2.0 2.2 1.5 1.0 1.5 0.6 1.0 3.0 5.0 5.0 1.0 0.1 5.0 3.0 1.0 4.0 Sum 100 100

Prescription Example  5: Massage cream

Formulation example of the massage cream in the cosmetic composition containing the Wasson extract of Preparation Example 1 is as shown in Table 6.

ingredient Content (unit: weight%) Edible Extract Lipophilic Monostearate Glycerin Stearyl Alcohol Stearic Acid Polysorbate 60 Sorbitastearate Isostearyl Isolate Squalane Mineral Oil Dimethicone Hydroxyethyl Cellulose Glycerine Triethanolamine Preservative, Flavor, Pigment Distilled Water 3.0 1.5 1.5 1.0 1.5 0.6 5.0 5.0 35.0 0.5 0.12 6.0 0.7 Micro balance Sum 100

Prescription Example  6: essence

Prescription example of the essence of the cosmetic containing the Waksong extract of Preparation Example 1 is shown in Table 7.

ingredient Content (unit: weight%) Warson Extract Glycerine Betaine Fiji 1500 Allantoin DL-Pantenol E.D.T-2A Benzophenone -9 Hydroxyethyl Cellulose Sodium Hyaluronate Carboxyvinyl Polymer Triethanolamine Octyldodecanol Octyldodeces -16 Ethanol Preservative , Incense, pigment distilled water 5.0 10.0 5.0 2.0 0.1 0.3 0.02 0.04 0.1 8.0 0.2 0.18 0.3 0.4 6.0 Sum 100

Prescription Example  7: pack

Prescription example of the pack in the cosmetic containing the zinnia extract is shown in Table 8.

ingredient Content (unit: weight%) Polyurethane Extract Polyvinyl Alcohol Cellulose Gum Glycerin Sebum 1500 Cyclostrin DL- Panthenol Allantoin Glyciric Acid Monoammonium Nicotinamide Ethanol Sebum 40 Cured Castor Oil Flavor, Pigment, Preservative Distilled Water 3.0 15.0 0.15 3.0 2.0 0.15 0.4 0.1 0.3 0.5 6.0 0.3 Micro balance Sum 100

Experimental Example 2. Skin barrier function recovery effect

In order to find out the effect of restoring the skin barrier function of the cosmetic composition containing the Wahsong extract of the present invention was carried out as follows. The experiment was repeated application of acetone to the hair of a hairless mouse to impair barrier function, and then measured epidermal water loss (TEWL) using servomed evaporimeter EP1 (Sweden) 4.0g / ㎡ / When it reaches h, after applying each of the nutrition cream of Example 1 containing the Waksong extract of the present invention prepared in Formulation Example 4 and the nutrition cream of Comparative Example 1 not containing the Waksong extract in an area of 5 cm 2, Epidermal moisture loss was measured after 1 hour, 2 hours, 4 hours, and 8 hours, and the extent of barrier function recovery was evaluated by evaluating the extent of decrease. In this case, a known lipid mixture (2: 1: 1 mixture of ceramide: cholesterol: fatty acid) was used as a positive control. The results are shown in Table 9 comparing the change over time based on the amount of epidermal moisture loss in the initial damaged barrier state (100%).

Epidermal moisture loss (%) sample 0 hours 1 hours 2 hours 4 hours 8 hours Example 1 85 63 55 39 20 Comparative Example 1 85 80 77 75 73 Positive control group (ceramide: cholesterol: fatty acid = 2: 1: 1) 88 66 60 50 29

As shown in the results of Table 9, in the case of Example 1 which is a cosmetic containing the Wahsong extract of the present invention, it was confirmed that the skin barrier function recovery effect is very excellent compared to Comparative Example 1, ceramide is a known lipid mixture: Significant results were obtained when compared to the positive control group of 2: 1: 1 mixture of cholesterol and fatty acid.

Experimental Example 3. Skin Moisturizing Improvement

In order to find out the effect of improving the skin moisturizing effect of the cosmetic containing the Wahsong extract of the present invention was carried out as follows. 25 people in their 20s to 40s without skin diseases are divided into two sets of 20 people per set, and each group contains the nourishing cream and the packs extract of Example 1, which contain the extracts prepared in Formula 4 above. The nutrition cream of Comparative Example 1, which is not, was applied twice a day for 1 month to the face and forearm. Measure the skin conductivity using the corneometer (CM820 courage Khazaka electronic GmbH, Germany) at constant temperature and humidity conditions (24 ° C, 40% humidity) before starting the application. Skin conductivity was measured to assess the rate of increase. The results are shown in Table 10 below.

Skin conductivity increase rate (%) sample 1 week later after 2 weeks 4 weeks later Example 1 57 63 58 Comparative Example 1 11 13 12

As shown in the results of Table 10, in the case of Example 1 which is a cosmetic containing the Wahsong extract of the present invention, the skin conductivity increase rate was very excellent compared to Comparative Example 1. In general, since the skin conductivity is proportional to the skin moisture content, it can be seen from the above results that the cosmetics containing the hawsong extract in the present invention also maintain a higher skin moisture content than the cosmetics. Therefore, the cosmetics containing the Wahsong extract of the present invention is excellent in improving the skin moisturizing power.

Experimental Example 4. Survey

In order to find out the effect of improving the skin moisturizing effect of the cosmetic containing the Wahsong extract of the present invention was carried out as follows. 25 people in their 20s and 40s without skin disease are divided into two sets of 20 people per set, and each group contains no nutrition cream and hawsong extract of Example 1, which contains the hawsong extract prepared in Prescription Example 4. Nourishing cream of Comparative Example 1 was applied twice a day for 1 month to face and forearm. Subjects were evaluated for subjective efficacy through a questionnaire survey on skin pulling, roughness, and elasticity after using Example 1 and Comparative Example 1. The questionnaire survey was to classify the satisfaction of the effect of the product for each item of skin pulling, roughness, elasticity change by 1-5 points (the higher the number, the higher the satisfaction), and the results are shown in Table 11 below.

sample Satisfaction (Average Score) Skin Pulling Improvement Skin Roughness Improvement Increased skin elasticity Example 1 4.3 4.8 4.8 Comparative Example 1 2.7 3.3 3.0

As shown in the results of Table 11, in the case of Example 1 which is a cosmetic containing the Waksong extract of the present invention of the present invention to improve the pull, roughness, gloss of the skin as compared to Comparative Example 1 does not contain the Waksong extract The effect was superior.

Claims (7)

A cosmetic composition for promoting the differentiation of keratinocytes of the skin, comprising the extract as an active ingredient. Cosmetic composition for restoring skin barrier function containing the seungsong extract as an active ingredient. Cosmetic composition for skin moisturizing containing the pine extract as an active ingredient. 4. The cosmetic composition according to any one of claims 1 to 3, wherein the Wasong extract contains 0.001-30.0% by weight based on the total weight of the composition as the active ingredient. The method according to any one of claims 1 to 3, wherein the vortex extract comprises (a) water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, chloroform, butyl acetate and 1,3-butylene Cosmetic composition, characterized in that extracted by using a solvent extraction method, (b) supercritical extraction method or (c) ultrasonic extraction method using an extraction solvent selected from the group consisting of glycol. The cosmetic composition according to claim 1, wherein the cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion Cosmetic composition, characterized in that the formulation is selected from the group consisting of foundation, wax foundation and spray. A cosmetic method according to any one of claims 1 to 6, wherein the cosmetic composition is applied to human skin.