KR102410502B1 - A Cosmetic Composition Having An Antioxidation, Anti-Inflamation Effects Contaning Rosemarimus Officinalis, Chrysanthemum Indicum Flower , Magnolia Kobus Flower , Polygonum Cuspidatum Root Extracts - Google Patents

Abstract

The present invention relates to a cosmetic composition for anti-inflammation, skin irritation relief, and antioxidant containing a mixed extract of rosemary, chrysanthemum, magnolia flower, and rhododendron as an active ingredient. Specifically, the mixed extract of the present invention contains NO, PEF2, IL -6, IL-8 production inhibitory effect and free radical scavenging ability are excellent, so there is no skin irritation or toxicity while exhibiting anti-inflammatory, antioxidant, skin irritation relief and skin trouble improvement.

Description

A Cosmetic Composition Having An Antioxidation, Anti-Inflamation Effects Contaning Rosemarimus Officinalis, Chrysanthemum Indicum Flower , Magnolia Kobus Flower , Polygonum Cuspidatum Root Extracts}

The present invention relates to a cosmetic composition for anti-inflammation, skin irritation relief, and antioxidant, characterized in that it contains a mixture of rosemary, chrysanthemum, magnolia flower, and rhododendron extract.

The present invention relates to a cosmetic composition containing rosemary, chrysanthemum, magnolia flower, and rhododendron extract, and more particularly, to a cosmetic composition having excellent anti-inflammatory, skin irritation relief, antioxidant effect and safe anti-inflammatory activity for the skin. .

Cosmetics are products used to protect the skin and to beautify and clean the skin, but components different from those for the purpose of protecting the skin are inevitably included in the composition for product formation. These ingredients include surfactants, preservatives, fragrances, sunscreens, and pigments, as well as various ingredients for imparting other effects and effects, and these ingredients generally cause various troubles such as inflammation, pimples, and edema on the skin. is known to cause In addition, sebum and sweat discharged from the body, fatty acids in cosmetics, higher alcohols, protein components, etc. are decomposed into toxic substances by the skin flora present on the skin, which may cause skin inflammation, It is a well-known fact that skin inflammation is also induced by ultraviolet rays from the sun. The skin exposed by this external environment is easily exposed to reactive oxygen species, and the generated reactive oxygen species attacks not only normal cell tissues or cell membranes, but also adjacent tissue cells and non-cellular components, causing the skin to eventually become inflamed. do.

The inflammatory response manifests itself as five phenomena: a feeling of redness, a pricking feeling, a burning hotness, swelling, and a change in tissue, which is characterized by a detrimental surrounding environment, i.e., external factors such as bacteria. It is a physiological reaction to protect the living body from intrusion of substances and mechanical damage.

The inflammatory response is activated when immune cells recognize foreign substances in the living body, and the activated immune cells start by secreting many factors mediating inflammation. This inflammatory phenomenon leads to a large increase in various types of polynuclear leukocytes (PMNs) and immune substances, and the increased cells can treat and defend by secreting various types of proteolytic enzymes and cytokines, which are inflammatory cell products. makes it possible Nitric oxide (NO), a potent inflammatory mediator, is a highly reactive biomolecule involved in neurotransmission, vasodilation, and cell-mediated immune response. It is produced from L-argine by NO synthase, particularly lipopolysaccharide (LPS). When stimulated with inducible NOS is expressed and NO is produced. The NO generated in this way activates the macrophages to generate pro-inflammatory cytokines such as interleukin (IL)-1β, IL-6, IL-8, and tumor necrosis factor (TNF)-α. Under appropriate conditions, normal function is restored after the initial state of inflammation has passed, but if the stimulant that stimulates inflammation does not disappear or is continuously made, chronic inflammation occurs as a result, resulting in more serious tissue damage. As such, excessive inflammation causes damage to cells and connective tissue by proteolytic enzymes, and damage to connective tissue reduces skin elasticity and causes wrinkles, as well as adversely affects cell regeneration and proliferation. It causes skin aging, and localized erythema and swelling appear on the skin. If the connective tissue is seriously damaged and the inflammatory response continues, it becomes much more difficult to restore the normal tissue structure and function.

In cosmetics, factors that cause skin side effects are always latent, and various studies have been conducted to solve them. Substances that have been used for the purpose of relieving irritation such as erythema or edema and inflammation are non-steroidal, such as flufenamic acid, ibuprofen, benzydamine, and indomethacin. , steroids include prednisolone, dexamethasone, etc., allantoin, azurene, ε-aminocyproic acid, hydrocortisone, licoric acid and its derivatives (β-glycylecinic acid, glycylecinic acid derivative), etc. It is known to have anti-inflammatory effects. However, the indomethacin, which is generally used as an anti-inflammatory agent, is a material that cannot be used in cosmetics, the amount of hydrocortisone is limited, and licoric acid and its derivatives are difficult to stabilize or have poor solubility. Anti-inflammatory agents known so far, such as difficult to achieve a practical effect, have problems in terms of skin safety or stability during cosmetic formulation, so their use is limited.

Therefore, as a natural material, there is no or little risk of such side effects or cytotoxicity, and it can effectively inhibit COX-2 and iNOS, so it has an excellent anti-inflammatory effect. The development of substances that can be used in cosmetics is required. In particular, in recent years, in order to meet the needs of consumers, research and development on cosmetics or cosmetic compositions using natural raw materials are being actively conducted.

Therefore, in this study, we tried to examine the potential as a cosmetic material that can prevent skin irritation reactions by inhibiting the production of inflammatory mediators, NO, PEF2, IL-6, and IL-8, through natural materials. In order to improve skin irritation caused by various stressors such as free radicals and skin troubles caused by inflammation, development of natural extracts with excellent antioxidant and anti-inflammatory effects and without side effects, development of cosmetics containing the extracts, and optimal extraction methods development is essential.

An object of the present invention is to provide a cosmetic composition having anti-inflammatory, anti-inflammatory, and anti-inflammatory activity that is safe for the skin while having excellent anti-inflammatory, skin irritation relief, and antioxidant effects.

In order to achieve the above object, the present invention is a mixture of rosemary, chrysanthemum, magnolia flower, and rhododendron extract, which has excellent anti-inflammatory and antioxidant effects, has no skin irritation or toxicity, and exhibits the effect of alleviating skin irritation and improving skin troubles. An object of the present invention is to provide a cosmetic composition for anti-inflammatory and anti-oxidation using a mixed extract as an active ingredient, and a method for preparing the same.

The mixed extract of the present invention is dried or undried rosemary, chrysanthemum, magnolia flower, minced and mixed, followed by adding an excess of an extraction solvent to extract under temperature and pressure conditions commonly used in the art, followed by filtration can be manufactured. In addition, the mixed extract of the present invention can be prepared by preparing single extracts of rosemary, chrysanthemum, magnolia flower, and hojanggeun in the same manner as above, and then mixing these single extracts. The extraction solvent used in the present invention is a solvent commonly used in the art, and is preferably at least one selected from purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane and hexane. When preparing the mixed extract of the present invention, rosemary, chrysanthemum, magnolia flower, and hojanggeun may be used, and only any specific part of the root, stem, and leaf may be used. Rosemary, chrysanthemum, magnolia flower, and hojanggeun used in the present invention can be obtained by a conventional method in the art.

The cosmetic composition according to the present invention may contain other ingredients that can give a synergistic effect to the main effect without impairing the main effect of the present invention. The content of the mixed extract of rosemary, chrysanthemum, magnolia flower, and rhododendron may be present in the composition in an amount of 0.001 to 50% by weight based on the freeze-dried weight of the entire composition, and when used at less than 0.001% by weight, the effect is weak and 50% by weight When used in excess of %, the effect does not increase significantly. The cosmetic composition of the present invention is not particularly limited in its formulation as an external preparation for the skin, and is not particularly limited in its formulation. It may be any type of cosmetics selected from among the cleansing cream, cleansing water, makeup remover, body cleanser, foam cleanser, shampoo, and detergent.

Hereinafter, the present invention will be described in detail through Examples and Experimental Examples, but these are only presented to help the understanding of the present invention, and the present invention is not limited thereto.

As described above, the extracts of rosemary, chrysanthemum, magnolia flower and hojang-geun of the present invention can be safely used without side effects on the skin while exhibiting excellent anti-inflammatory, skin irritation relief, and antioxidant effects.

Hereinafter, the present invention will be described in more detail through examples. The following examples are only described for the purpose of expressing the present invention more clearly, but the content of the present invention is not limited to the following examples.

(Comparative Example 1)

Preparation of Rosemary Extract

Add 10 to 20 times purified water to the chopped and dried rosemary raw material, extract at 80°C for 2 hours, and naturally cool to room temperature. The precipitate was removed using a centrifuge and the supernatant was collected or the supernatant filtered twice with filter paper (Hyundai Filter Paper No. 53, 110mm) was used as an extract.

(Comparative Example 2)

Preparation of Chrysanthemum Extract

Add 10 to 20 times purified water to the chopped dried chrysanthemum raw material, extract at 80°C for 2 hours, and naturally cool to room temperature. The precipitate was removed using a centrifuge, and the supernatant was collected or the supernatant filtered twice with filter paper (Hyundai Filter Paper No. 53, 110mm) was used as an extract.

(Comparative Example 3)

Preparation of Magnolia Flower Extract

10% ethanol is added to the chopped and dried magnolia flower raw material, extracted at 70°C for 4 hours, and then cooled naturally to room temperature. The precipitate was removed using a centrifuge, and the supernatant was collected or filtered twice with filter paper (Hyundai Filter Paper No.

(Comparative Example 4)

Preparation of rhizome extract

70% ethanol is added to the chopped and dried raw material of rhizome, extracted at 70°C for 4 hours, and then cooled naturally to room temperature. The precipitate was removed using a centrifuge, and the supernatant was collected or filtered twice with filter paper (Hyundai Filter Paper No.

Manufacture of mixed extracts of rosemary, chrysanthemum, magnolia, and rhododendron

Chopped and dried rosemary and chrysanthemum were extracted by adding 10 to 20 times purified water to each raw material and extracted for 2 hours at 80 ° C. Cool naturally to room temperature. Each extract was removed by using a centrifuge to remove the precipitate, and the supernatant was collected or filtered twice with filter paper (Hyundai Filter Paper No. . A mixture of 1-5: 1-5: 1-5: 1-5 was used as a mixed extract.

(Experimental Example 1)

Antioxidant activity measurement

A 400 uM DPPH (2.2-diphenyl-1-picryl hydrezyl) solution was dissolved in 80% ethyl alcohol, filtered, and then used immediately. The prepared DPPH solution and the sample solution were mixed 1:1 and vigorously, and then left in a dark place at room temperature for 20 minutes. After measuring the absorbance at 540 nm, the DPPH radical removal rate (%) was calculated through Equation 1 below, and the calculated DPPH radical removal rate is shown in Table 1 below. In this case, ascorbic acid at 10 ug/ml was used as a positive control to remove radicals. As shown in Table 1, it was confirmed that the antioxidant activity of the mixed extract of Example 1 was increased compared to Comparative Examples 1-4.

(Equation 1)

DPPH radical scavenging rate (scavenging, %) = (A - B) / A x 100

In the above formula, A is the absorbance obtained from the blank test solution, and B is the absorbance obtained from the test solution.

Antioxidant activity of rosemary, chrysanthemum, magnolia flower, rhododendron and mixed extracts density(%) Antioxidant power (%) ascorbic acid
10ug/ml Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Example 1 0.1 93 73 51 47 67 86 One 90 91 88 88 95

(Experimental Example 2)

anti-inflammatory effect

(Experimental Example 2-1)

Inhibition of NO production

RAW264.7 macrophages were purchased from the Korea Cell Line Bank and were treated with 10% FBS and 1% Antibiotic-Antimycotic (10 units/mL penicillin, 100 µg/mL sterptomycin , 0.25 µg /mL amphotericin) at 37 °C and 5% CO2. It was cultured in this added DMEM medium.

To measure the anti-inflammatory effect, NO production was measured. The amount of NO production was determined in the cell culture medium through the Nitrite/Nitrate Assay Kit (Invitrogen, USA) using Griess reagent.

was analyzed by measuring NO2-, a stable oxide of NO. RAW264.7 cells were inoculated in a 96-well plate at a concentration of 2 × × 10 ⁶ cells/ml, cultured for 24 h, the medium was discarded, washed with PBS, and then exchanged with a new medium not containing FBS. The extract was treated with LPS (100ng/mL) and cultured again for 24 h. 100 μL of cell culture supernatant and 100 μL of Griess reagent were mixed and reacted for 10 min in a 96-well plate, and absorbance was measured at 540 nm. The results were quantified by obtaining a standard curve using NaNO ₂ . As shown in Table 2, it was confirmed that the anti-inflammatory activity of the mixed extract of Example 1 was increased compared to Comparative Examples 1-4.

Anti-inflammatory activity of rosemary, chrysanthemum, magnolia flower, rhododendron and mixed extracts density(%) NO conc (ug/ml). LPS(-) LPS(+) Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Example 1 0.01

0.2
13.4 10.5 10.7 11.5 12.0 7.5 0.1 8.7 9.9 10.8 7.4 0.5 One 0.7 1.9 3.5 0.5 0.2

(Experimental Example 2-2)

IL-6, IL8, PGE2 production inhibitory effect

RAW264.7 macrophages were purchased from the Korea Cell Line Bank and were treated with 10% FBS and 1% Antibiotic-Antimycotic (10 units/mL penicillin, 100 µg/mL sterptomycin , 0.25 µg /mL amphotericin) at 37 °C and 5% CO2. It was cultured in this added DMEM medium.

The following experiment was performed to determine the anti-inflammatory effect of rosemary, chrysanthemum, magnolia, and rhododendron extracts by measuring the production inhibitory effect of PGE2, IL-6 and IL-8, which are inflammatory mediators. RAW264.7 macrophages were inoculated into a 6-well culture plate at a density of 1 x 105 cells, incubated in an incubator at 37 °C, 5% CO2 for 24 hours, and then treated with 500uM hydrogen peroxide (H2O2) to stimulate the wells for 24 hours. After giving, the extract was treated and reacted for 48 hours, the culture medium was collected and an ELISA assay was performed. Using, it was tested according to the menu of each company (P. Tijssen, in Practise and Theory of Enz. Immunoassays, Elsevier, Amsterdam, 1985), and the production of PGE2, IL-6 and IL-8 using the following Equation 3 The inhibitory effect was obtained, and the results are shown in Table 9 below. As a result of the experiment, as shown in Table 3, Comparative Examples 1 to 4 and Example 1 both inhibited the production of PGE2, IL-6 and IL-8 in a concentration-dependent manner, and compared to Comparative Examples 1 to 4, the mixture of Example 1 It was found that the anti-inflammatory activity of the extract was excellent.

(Equation 2)

Inhibitory effect (%) = (A-B) /A X 100

A: Production amount of PGE2, IL-6 or IL-8 in the hydrogen peroxide (H2O2) treatment group (pg/ml)

B: hydrogen peroxide (H2O2) treated Comparative Examples 1 to 4, Example 1 of PGE2, IL-6 or IL-8 production amount (pg / ml)

Anti-inflammatory activity of rosemary, chrysanthemum, magnolia flower, rhododendron and mixed extracts sample PGE2 IL-6 IL-8 Conc.
(pg/ml) Inhibition
(%) Conc.
(pg/ml) Inhibition
(%) Conc.
(pg/ml) Inhibition
(%) control 151.1 22.8 22.8 H2O2 (500uM) 487.8 0 191.1 0 322.4 0 H2O2+0.1% of Comparative Example 1 365.7 25.0 155.4 18.7 178.8 44.5 1% of H2O2 + Comparative Example 1 211.0 56.7 125.4 34.4 155.2 51.9 0.1% of H2O2+Comparative Example 2 298.8 38.7 128.5 32.8 231.9 28.1 1% of H2O2+Comparative Example 2 165.5 66.1 105.9 44.6 181.0 43.9 0.1% of H2O2+Comparative Example 3 387.5 20.6 165.8 13.2 288.7 10.5 1% of H2O2 + Comparative Example 3 365.7 25.0 157.9 17.4 278.8 13.5 0.1% of H2O2+Comparative Example 4 221.4 54.6 121.6 36.4 188.1 41.7 1% of H2O2+Comparative Example 4 158.3 67.5 105.6 44.7 116.1 64.0 H2O2+0.1% of Example 1 200.5 58.9 100.6 47.4 178.4 44.7 H2O2+1% of Example 1 135.0 72.3 51.8 72.9 107.7 66.6

(Experimental Example 3)

Stimulus Relief Assessment

RAW264.7 macrophages were purchased from the Korea Cell Line Bank and contained 10% FBS and 1% Antibiotic-Antimycotic (10 units/mL penicillin, 100 μμg /mL sterptomycin, 0.25 μμg /mL amphotericin) under the conditions of 37°C and ₅ % CO2. ) was cultured in DMEM medium. To measure the alleviation effect, RAW264.7 macrophages were stimulated with LPS (100 ng/mL) and then treated with the extract to measure the cytotoxicity inhibitory effect of LPS toxicity by MTT assay. Cell viability was analyzed using the principle of MTT, which is reduced to purple formazan by mitochondrial dehydrogenase in living cells. RAW264.7 cells were inoculated in a 96-well plate at a concentration of 2 × × 10 ⁴ cells/mL, cultured for 24 h, then the medium was discarded, washed with Phosphate Buffered Saline (PBS), and replaced with a new medium not containing FBS. . Cells were treated with each herbal extract, either stimulated with LPS (100 ng/mL) or unstimulated, and cells were incubated for 24 h. Then, 100 μL of 0.5% MTT solution was added to each well for 4 h. incubated during After removing the culture medium, 200 μL of DMSO was added, shaken for 10 min, and absorbance was measured at 570 nm. Cell viability was expressed as a percentage by dividing the absorbance value relative to the control. As shown in Table 4, each of Comparative Examples 1 to 4 had a cytotoxicity alleviation effect by LPS, but it was confirmed that the cytotoxicity alleviating effect by LPS in the mixed extract of Example 1 was superior.

Stimulation-relieving activity of rosemary, chrysanthemum, magnolia flower, rhododendron and mixed extracts density(%) Cell viability (ug/ml). LPS(-) LPS(+) Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Example 1 0.1 100 78 91 94 85 95 104

(Experimental Example 4)

Stimulus relief clinical evaluation

For clinical evaluation of irritation relief of a cosmetic composition comprising a mixed extract of rosemary, chrysanthemum, magnolia flower, and magnolia flower of the present invention, a cosmetic composition was prepared with the composition shown in Table 4 below. A cosmetic composition (Formulation Example 1) containing a mixed extract of rosemary, chrysanthemum, magnolia flower and hojanggeun prepared by the method of Example 1 and a nutritional lotion that does not contain a mixed extract of rosemary, chrysanthemum, magnolia flower, and hojanggeun ( Comparative Formulation Example 1) was prepared and irritation relief clinical evaluation was performed.

Raw material name Formulation Example 1 Comparative Formulation Example 2 One Mixed extract of rosemary, chrysanthemum, magnolia flower, and rhododendron (Example 1) 1.0 - 2 stearic acid 4.0 4.0 3 cetyl alcohol 1.0 1.0 4 Glyceryl Monostearate 2.0 2.0 5 Polyoxyethylene sorbitan monostearate 0.5 0.5 6 Sorbitan sesquioleate 0.5 0.5 7 Glyceryl Monostearate/Glyceryl Stearate/Polyoxyethylene Stearate 1.0 1.0 8 wax 1.0 1.0 9 liquid paraffin 4.0 4.0 10 squalene 4.0 4.0 11 Carlylic/Capric Triglycerides 4.0 4.0 12 Carboxyvinyl Polymer 0.3 0.3 13 butylene glycol 5.0 5.0 14 glycerin 3.0 3.0 15 triethanolamine 0.5 0.5 16 Sodium-EDTA 0.02 0.02 17 Purified water remaining amount remaining amount 18 water - 1.0

Manufacturing method

Formulation Example 1: After mixing and stirring No. 12, 13, 14, and 17, heated to between 80 and 85° C. and added to the manufacturing unit, the emulsifier was activated, and 2, 3, 4, 5, 6, 7, 8, 9 , 10, 11 and 16 are heated to between 80 and 85 ℃ to dissolve, 15 is added and stirred to put into the manufacturing unit and emulsified. When the emulsification is finished, it is cooled to 35°C while stirring using a stirrer, put in No. 1, cooled to 25°C, and then aged.

Comparative Formulation Example 1: After mixing and stirring No. 12, 13, 14 and 17, heated to between 80 and 85° C. and put into the manufacturing unit, the emulsifier was activated, and 2, 3, 4, 5, 6, 7, 8, No. 9, 10, 11, and 16 are heated to between 80 and 85° C. to dissolve, then 15 is added and stirred to put into the manufacturing unit and emulsify. After the emulsification is finished, it is cooled to 35°C while stirring using a stirrer, and then cooled to 25°C by adding No. 18 and then aged.

The irritation-relieving effect of the cosmetic prepared above was confirmed through a simple clinical evaluation.

To evaluate the skin irritation improvement effect of the formulation example cosmetic, the following experiment was conducted on 10 female volunteers in their 20s and 30s. In order to make the skin irritation measurement conditions of the volunteers the same, the test site was kept clean and dry, and the skin was stabilized in a place where constant temperature and humidity (20 °C, relative humidity 40-60%) was maintained for at least 30 minutes. . For the measurement of skin irritation, Formulation Example 1 was applied to the left and Comparative Formulation Example 1 to the right centering on the phosphorus, and the degree of skin irritation was evaluated through questionnaire evaluation after 20 minutes. Irritation measurement was conducted through self-question evaluation of volunteers based on the contents described in Tables 6 and 7 below, and the difference in skin irritation was compared with Formulation Example 1 and Comparative Formulation Example 1.

Skin irritation evaluation criteria 0 One 2 3 no irritation there is a little usually with irritation

Skin irritation evaluation items and questionnaire Erythema Edema Itching Prickling

Table 8 shows the survey results.

Skin irritation survey results classification Erythema Edema Itching Prickling Formulation example 0 0 0.5 0.3 Comparative formulation example 0 0 2.0 1.8

As shown in Table 8, the cosmetic of Formulation Example 1 containing a mixed extract (Example 1) of rosemary, Chrysanthemum chrysanthemum, magnolia flower, and magnolia was stimulated compared to that of Comparative Formulation Example 1 prepared by correcting water instead of the extract It was confirmed that the mitigation effect was very good.

Hereinafter, a method of manufacturing various cosmetic products prepared by containing a mixed extract of rosemary, chrysanthemum, magnolia flower, and hojanggeun will be described.

(Formulation Example 2)

Softening lotion (skin lotion)

As shown in Table 9 below, a softened lotion containing a mixed extract of rosemary, chrysanthemum, magnolia flower, and hojanggeun was prepared according to a conventional method.

Raw material name weight% Mixed extract of rosemary, chrysanthemum, magnolia flower, and hojanggeun (Example 1) One glycerin 3.0 butylene glycol 2.0 propylene glycol 2.0 carboxyvinyl polymer 0.1 PEG-12 Nonylphenyl Ether 0.2 Polysorbate 80 0.4 ethanol 10 triethanolamine 0.1 Sodium-EDTA 0.02 Preservatives, Colors, Flavors appropriate amount Purified water to 100

(Formulation Example 3)

Milk lotion (emulsion)

As shown in Table 10 below, a milk lotion containing a mixed extract of rosemary, chrysanthemum, magnolia flower, and hojanggeun was prepared by heating the aqueous and oil phases at 75° C., respectively, and emulsifying them for 5 minutes, cooling and defoaming.

Raw material name weight% Mixed extract of rosemary, chrysanthemum, magnolia flower, and hojanggeun (Example 1) 2 squalane 5.0 beeswax 4.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.5 liquid paraffin 0.5 Caprylic/Capric Triglycerides 5.0 glycerin 3.0 butylene glycol 3.0 propylene glycol 3.0 carboxyvinyl polymer 0.1 triethanolamine 0.2 Sodium-EDTA 0.02 Preservatives, Colors, Flavors appropriate amount Purified water to 100

(Formulation Example 4)

nourishing cream

As shown in Table 11 below, the nourishing cream containing the mixed extract of rosemary, chrysanthemum, magnolia flower, and hojanggeun was prepared by heating the aqueous and oil phases at 75° C., respectively, and emulsifying them for 5 minutes, cooling and defoaming.

Raw material name weight% Mixed extract of rosemary, chrysanthemum, magnolia flower, and hojanggeun (Example 1) 3 beeswax 10.0 Polysorbate 60 1.5 sebum 60 hydrogenated castor oil 2.0 Sorbitan sesquioleate 0.5 liquid paraffin 10.0 squalane 5.0 Caprylic/Capric Triglycerides 5.0 glycerin 5.0 butylene glycol 3.0 propylene glycol 3.0 triethanolamine 0.2 Carboxyvinyl Polymer 0.2 Sodium-EDTA 0.02 Preservatives, Colors, Flavors appropriate amount Purified water to 100

Claims (5)

A cosmetic composition comprising a mixture of rosemary, chrysanthemum, magnolia flower, and rhododendron extract as an active ingredient, characterized by anti-inflammatory, skin irritation relief and antioxidant effects
The cosmetic composition according to claim 1, wherein the extract is contained in an amount of 0.001 to 50% by weight based on the total weight of the composition.
The method according to claim 1, wherein the extract is a leachate from which the raw material has been leached; concentrate of the leachate; Or a dry powder of the extract or concentrate, anti-inflammatory, skin irritation relief and antioxidant cosmetic composition
The cosmetic composition according to claim 1, wherein the extraction solvent of the extract uses at least one extraction solvent selected from purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane and hexane.
According to claim 1, wherein the cosmetic composition is any cleansing cream, cleansing water selected from among lotion (skin lotion), nourishing lotion, essence, nourishing cream, massage cream, nourishing essence, pack, makeup base, foundation, moisturizing oil and makeup base. , makeup remover, body cleanser, foam cleanser, and shampoo, characterized in that the cosmetic composition has anti-inflammatory, skin irritation relief, and antioxidant effects