KR20090038085A - Cosmetic composition - Google Patents

Abstract

A cosmetic composition is provided to improve skin trouble caused by free radical and inflammation without side effects by using artemisiae asiaticae Herba, brassica juncea Cosson, Caesalpinia Sappan, schizandrae Fructus, sophorae Flos, curcumae longae Rhizoma, Platicodi Radix, condonopsis lanceolata Trautv, Rhynchosia volubilis and Perillae Semen. A cosmetic composition is extracted from the mixture consisting of artemisiae asiaticae Herba 1-5 weight%, brassica juncea Cosson 1-5 weight%, Caesalpinia Sappan 3-7 weight%, schizandrae Fructus 3-7 weight%, sophorae Flos 5-10 weight%, curcumae longae Rhizoma 5-10 weight%, platicodi Radix 10-15 weight%, condonopsis lanceolata Trautv 5-10 weight%, Perillae Semen 3-7 weight% and Rhynchosia volubilis 3-7 weight%. The mixture is extracted in at least solvent selected from water, ethanol and 1,3 butylene glycol.

Description

Cosmetic composition {Cosmetic composition}

The present invention relates to a cosmetic composition containing an extract of a natural product.

One of the representative causes of skin trouble is free radicals present in the human body. Free radicals present in the human body can cause various problems such as wrinkles, skin cancer, cell death, rheumatoid arthritis, atopic dermatitis, and acne by destroying cells, cutting off connective tissue in the dermis, or cross-linking. These radicals are produced by the phagocytosis of leukocytes, the electron transport system during the production of ATP in mitochondria, the action of Myeloper Oxide (MPO), ultraviolet light, tobacco, normal metabolic processes, stress, pollutants, bacteria and the like. Of course, the human body includes antioxidants (SuperOxide Dismatase (SOD), catalase, vitamin E, vitamin C, ubiquinol), but these antioxidants include age, pollution, ultraviolet light, Broken by stress, radicals gradually increase.

Another major cause of cutaneous travel is the inflammatory response. Inflammation is a physiological response that protects the body from harmful environmental conditions, such as invasion and mechanical damage of foreign objects such as bacteria. These inflammations increase various types of polymorphonuclear leukocytes (PMNs) and immune substances, and these cells treat and defend by releasing various types of proteolytic enzymes and cytokines, which are inflammatory cell products. In particular, enzymes such as Elastase, hyalurinidase and lipoxygenase break down proteins and lipids produced during inflammation, but their actions sometimes cause detrimental damage to adjacent tissue cell and non-cellular components. Therefore, inflammation is restored to normal function after an initial condition under certain conditions, but if inflammation irritant is not eliminated or made continuously, chronic inflammation occurs and causes more serious tissue damage. As a result, cells and connective tissues are damaged by proteolytic enzymes caused by excessive inflammation, and the damage of these connective tissues decreases the elasticity of the skin and causes wrinkles, and furthermore, regeneration and proliferation of cells. It will have a bad effect, leading to rapid skin aging.

Therefore, in order to improve skin troubles caused by free radicals and inflammation, it is necessary to develop a material having no side effects and low cost, and to develop an easy-to-use cosmetic containing the material.

On the other hand, Artemisia princeps Pamp. Is a perennial plant of Asteraceae (Compositaea), commonly called wormwood, wormwood, and dried leaves or leaves are called larvae. The outpost contains essential oils, tannins, bitter substances, artemic acid, ascorbic acid, carotene, and leaves contain cineol, alpha-tuyon, borneool, paraffin, adenine, choline, vitamins A, B, C, and D. It is known to be used as an astringent hemostatic drug and analgesic medicine in uterine bleeding, abdominal pain, and bloody stool, and as a tonic hematopoietic medicine (Commercial medicinal plant guide, Medical Plant Research Society, Shinil Corp., 2000).

Mustard ( Brassica juncea Cosson ) is a biennial plant of the Brassica family, the seeds contain glycosides sinigrin and hydrolase myrosine and about 37% fatty oil. Powdered seeds and vinegar are used to treat pneumonia, arthritis, neuralgia, rheumatism, etc., and decoction of water is used to detoxify mushrooms or poison the animal. White mustard seeds are used for Jinhae, expectorant, stomachache and stomach ache. When mustard oil is mixed with fatty oil and applied to the skin, it has a stimulating effect. As a result, it is known to improve blood vessel dilation and blood circulation (Medicinal ingredients and use, Encyclopedia of Science, January, 1991).

Fire wood ( Caesalpinia sappan L.) belongs to the legumes (Leguminosae), cut off the main stem of the tree, cut the bark and the edges of the wood, and only dry wood flesh is used as a medicine. The bark contains about 2% of the colorless primary pigment, brazilin, which is oxidized in contact with air to form brasilein. In addition, firewood includes saponins, and essential oils and tannins based on α-L-phellandrene and ocimene. These firewood trees have been used for pain relief in motion therapy, and have been used in wiccardar and erythema as astringent hemostatic agents. In addition, blood coagulation, central nervous system inhibition, antibacterial activity and vasoconstriction of fire throat have been reported (medicinal ingredients and use, encyclopedia of scientific publication, Ilwol Seokje, 1991).

Schizandra chinensis Baillon) is the fruit of Schisandra chinensis tree schisandraceae (Schizabdraceae), organic acids (remonsan 10 ~ 13%, malic acid 7-10%, wine acid 0.8%), sugar 1.5%, tanninjil, anthocyanin pigments, pectin, oil, resin 8 %, Saponin about 5%, ascorbic acid 200 ~ 600 mg% and the like. Seeds contain antioxidant lignan compounds, such as cyanidrin A, B, C, cyandrrol A, B gomycin B, C, D, F, G, czandrella A, B, and fruited (swords). The red), the higher the content of lignan compound. Omija has a strong taste and astringent effect, which regulates the skin's sweat glands, serves as a defense, promotes the production of essence, quenches thirst, and lowers blood sugar. It is also effective in removing wrinkles and skin elasticity. Schisandra chinensis boiled in water has been known to promote hair growth and prevent baldness. (Medicinal ingredients and use of herbs, Encyclopedia of Scientific Encyclopedia, Janwolseogak, 1991 / Book of Commercial Medicinal Plants, Medical Plant Research Group Hen, Shin Il Co., 2000).

Sophora japonica L. is a deciduous tree of the legumes (Leguminosae), with buds containing 10-28% of rutin, a small amount of camphorol, quercetin, sophoroside, genistein glycosides, and prosapogenin-like substances. (Berulin + sophoradiol + glucose + glucuronic acid). Rutin has capillary potentiation, and Rutin, Quercetin and Myrcitrin have strong heart, diuretic, antihistamine and vitamin C coordination. Therefore, dehydrated water is an anti-inflammatory, astringent, anti-diabetic, and hemostatic drug that is known to be effective for inflammatory bleeding, hemostasis, nosebleeds, and hemorrhoids (components and uses of herbs, scientific encyclopaedia 1991).

Curcuma longa L. is a perennial herb of the Zingiberaceae family that grows in the tropics. The root stem contains 0.4 to 0.65% of curcumin, a yellow pigment, and 6-10% of essential oils. The essential oils include sesquiterpene 65.5%, sesquiterpene alcohol 22%, d-camphor 2.5% and d-camphor, turumeron and para-torylmethylcarbinol. In addition, turmeric contains alpha-pinene, azulene, beta-carotene, cinnamic acid, curcumin, eugenol, 1,8-cineol, para-coumaric acid, para-cymene, vanillic acid, calcium, iron, magnesium, phosphorus, Potassium, zinc, vitamins B1, B2, B3, C and more. It is reported that turmeric has anti-inflammatory and analgesic effects on early symptoms of boils and has strong antibacterial, antibiotic, anti-cancer effects (components and uses of herbs, Encyclopedia of Science, January, 1991).

Gilkyung ( Platycodon grandiflorum (Jacq) Nakai) is a perennial herb of the Campanulaceae, whose root contains about 2% of bellflower saponins, berullins, coumarins, resins, oils, alkaloids, and inulin. The bellflower saponin stimulates the mucous membranes in the throat and stomach and reflexively stimulates the secretion of the bronchial gland, resulting in sputum cutting.It is used for central suppression, anti-inflammatory, vasodilation, and anticholinergic activity, mainly for sedation, analgesic and antipyretic effect. It has been used for anti-inflammatory activity, especially when the sore throat, has been used since ancient times (components and use of herbs, scientific encyclopedia publishing, Ilwolseok, 1991 / commercial medicinal plant illustration, drug plant research, Shinil Corporation, 2000).

Codonopsis lanceolata Trautv.) is a vine perennial herb of the Campanulaceae, whose roots include saponin, inulin and flavonoids on its leaves. This deodeok has a dry mouth, fever in the lungs, cough and phlegm to the hematopoiesis, and its water extract is known to inhibit skin fungi. (Ingredients and Uses of Herbs, Scientific Encyclopedia Publishing House, Ilwol Seokgak, 1991 / Illustrated Commercial Plants, Pharmaceutical Plant Research Society, Shinil Corporation, 2000).

Glycine soja Sieb. Et Zucc. Is a plant of the legumes (Leguminosae), which contains 18-22% fatty oil, 30-45% protein, vitamins, and isoflavon. Here, isoflavones (coumestrol, daidzein, daidzin, genistein, genistin) show collagen synthesis and hyaluronic acid synthesis enhancing effects. In addition, the anthocyanin component of Seomoktae exhibits antioxidant and collagen function-improving effects, and it is known that linoleic acid, which accounts for 50% of its soybean fat, has vascular strengthening effect, anti-acne, and anti-atopic effect (Korea Herbal Medicine, Kyohaksa, 2000 / http://www.naver.com).

Sesamum indicum L. is a sesame (Pedaliaceae), and its seeds are called sesame seeds and are used as cooking oil and medicinal. The types are divided into black horses (black sesame) and white horses (white sesame). This is also called a black man. This black seizure includes ceranium, sesamol, sesaminol, tocopherol and essential amino acids. Among these, tocopherol and essential amino acids, which are good for energy, have an excellent effect on the activity of DNA, which is a cell construct, and the prevention and treatment of dementia. have. In particular, M-100 is a new antioxidant that has been recognized by the academic community as a report on carcinogenesis. (Medicinal ingredients and uses, Encyclopedia of Scientific Encyclopedia, Ilwol Seokgak, 1991 http: //www.naver. com).

Until now, substances commonly used for anti-oxidation, anti-inflammatory and atopic skin improvement are known to have free radical scavenging effects such as ascorbic acid, α-tocopherol, and SOD. There is a problem that it is difficult to obtain a practical effect.

It is an object of the present invention to provide a novel cosmetic material having anti-inflammatory, anti-oxidant and atopic dermatological action at the same time and usefully used for skin troubles.

In addition, an object of the present invention is to provide a cosmetic composition comprising a new cosmetic material having the above efficacy.

The present invention provides a cosmetic composition containing the extract of the five leaves of the five generations (애 方 生氣 丹) leaf, mustard, fire wood, Schisandra chinensis, chunks, turmeric, Gilkyung, deodeok, Seomoktae and black sesame.

In the present invention, the five-season podium means that two natural colors of five colors (blue, red, yellow, white, black), which are a part of the yin and yang five-year-old traditional medicine, are selected and mixed for each color. It means a mixture of mustard, fire wood, Schisandra chinensis, chrysanthemum, turmeric, Gil Gyeong, deodeok, Seomoktae, and black sesame.

The ten natural products used in the cosmetic composition of the present invention may be directly collected from an acid or field, or purchased and used on the market.

The cosmetic composition of the present invention includes a mixture of 10 natural products mentioned above in various weight percent ratios. Preferably 1-5% by weight of young leaves, 1-5% by weight of mustard, 3-7% by weight of firewood, 3-7% by weight of Schizandra chinensis, 5-10% by weight, 5-10% by weight of turmeric, 10-15 weight %, Deokdeok 5-10% by weight, Seomoktae 3-7% by weight and the mixture contained a dry weight ratio of 3-7% by weight black sesame.

The five-pronged seed extract contained in the cosmetic composition of the present invention can be extracted by a solvent used as a natural extraction solvent to those skilled in the art, specifically, at least one solvent selected from the group consisting of water, ethanol and 1,3 butylene glycol Can be extracted.

In addition, the five-pronged base extract contained in the cosmetic composition of the present invention can be extracted by a bath extraction method, supercritical fluid extraction method and the like which are known extraction methods of natural products. In addition, the five generations extracts contained in the cosmetic composition of the present invention can be extracted using the ultrasonic method.

In general, ultrasound refers to sound waves having a frequency higher than the human audible frequency range of 16 Hz to 16 kHz (> 16 kHz). When ultrasonic waves are generated, the cavitation generated when the ultrasonic waves pass through a medium It affects the reaction and promotes the chemical reaction. (Song Jong-Ryul, Moses Young, Man-Soo Kim, Chan-Koo Park "Treatment of Refractory Organic Substances in Aqueous Solutions by Ultrasonic Irradiation", Korean Society of Environmental Engineering, Autumn Paper, 40-45 (1995))

Ultrasonic extraction is completed in a very short time compared to solvent extraction, and the extraction time is reduced by more than 10 times compared to the supercritical fluid extraction process (Chung KW, WI Kim, IK Hong and KA Park, "Ultrasonic energy effects on squalene extraction from amaranth seed ".Applied Chemistry. 4 (2), 149-152 (2000), Kim WI, KW Chung, SB Lee, IK Hong and KA Park," Ultrasound Energy Effects on Solvent Extraction of Amaranth Seed Oil ". J. Korean Ind. Eng. Chem. 12 (3), 307-311 (2001)).

Therefore, the present invention provides a cosmetic composition containing the extract of five generations by using the ultrasonic extraction method. The cosmetic composition of the present invention specifically has an ultrasonic wave length of 35 to 45 kHz, preferably about 40 kHz, and its output contains an ovarian progenitor extract prepared using ultrasonic waves under a condition of 300 to 500 Watts. Specific examples of the preparation method of the extract by the ultrasonic extraction method are as follows.

1) Cut the apple leaf, mustard, firewood, turmeric, gilkyung, and deodeok into a certain size, mix it with Seomoktae, black sesame, schisandra and lump, put it in hemp cloth, mix it with the extraction solvent, and soak for about 3 hours.

2) Put the mixture in the ultrasonic extractor to operate the ultrasonic wave. Adjust the position of the medicine so that the ultrasound can be delivered evenly to the medicine.

3) After that, the temperature of the extractor is maintained at 40 ℃ and the output of ultrasonic wave is raised to 300watt,

4) The temperature of the extractor is maintained at 50 ℃ and the output of ultrasonic wave is increased to 500watt and extracted for about 1 hour.

5) Then, remove the medicinal herbs and filter the extract to 0.8um to obtain the extract of the five stages of the present invention.

In the present invention, the five-pronged proximal extract contained in the cosmetic composition may be added in an amount of 0.1% to 10% as the weight of the liquid, and the solvent that is evaporated is recovered by using a distillation apparatus equipped with a cooling condenser. As dry weight, it can be added to cosmetics in the quantity of 0.001-5 weight%, Preferably it is 0.01-3 weight%.

In addition, the cosmetic composition of the present invention provides a cosmetic composition that is effective in improving antioxidant, anti-inflammatory and atopic skin.

The cosmetic composition of the present invention is not particularly limited in its formulation. For example, it may have a formulation such as a lotion, nutrition lotion, nutrition cream, massage cream, essence, pack.

In this regard, the cosmetic composition of the present invention may be other ingredients according to the formulation or purpose of use other than the five ginseng seedling extract (apple leaf, mustard, fire wood, Schisandra chinensis, fire lump, turmeric, Gilkyung, deodeok, Seomoktae, black sesame mixed extract) It can select arbitrarily and mix | blend.

Cosmetic composition containing a mixed extract of the five generations of the present invention (apple leaf, mustard, fire wood, Schisandra chinensis, lump, turmeric, Gilkyung, deodeok, Seomoktae, black sesame) has antioxidant, anti-inflammatory and atopic skin improvement effect, antioxidant, anti-inflammatory It can be used as a cosmetic for improving skin troubles.

Hereinafter, the configuration and effects of the present invention will be described in detail with reference to Examples and Experimental Examples, but the present invention is not limited thereto.

Example  1. Ultrasound Five Bangs  Vigorous extract manufacture

Cut 30 g of young leaves, 30 g of mustard, and 40 g of firewood to a certain size, and put 500 g of a mixture of Schisandra chinensis 60g, 60g of lumps, 60g of turmeric, 60g of Gilkyung 70g, Deodeok 50g, 50g of Seomoktae, and 50g of black sesame seeds in 1500g of purified water, 250g of ethanol. And 750 g of 1,3 butylene glycol were mixed with 2500 g of a mixed solvent and deposited for about 3 hours.

The mixture was placed in an ultrasonic extractor and the ultrasonics were operated. After adjusting the position of the medicine so that the ultrasonic waves can be evenly delivered to the medicine, the temperature of the extractor is maintained at 40 ° C. and the output of the ultrasonic wave is raised to 300 watts, after applying ultrasonic waves for 1 hour, the temperature of the extractor is maintained at 50 ° C. The ultrasonic power was raised to 500 watts and extracted for about 1 hour. Thereafter, the extractor was operated to cool the extract to room temperature, and the medicinal herbs were removed. The extract was filtered to 0.8 um to obtain an extract of five generations. The filtered fibrils were left at room temperature for 1 week, filtered through a 0.45 um filter and completely concentrated at 50 ° C. using a vacuum concentrator to obtain a dry weight of 156.3 g.

Example  2 ~ 5. Using ultrasonic wave and various extraction solvents Five Bangs  Vigorous extract manufacture

Examples 2 to 5 were used in Example 1, except that 1500 g of purified water, 250 g of ethanol, and 750 g of 1,3 butylene glycol were used instead of the solvent shown in Table 1 below. Extracted in the same manner as 1 to obtain a five-progeny extract. The yield according to the extraction solvent is shown in Table 1 below.

menstruum Yield (g) Example 2 water 116.4 Example 3 ethanol 160.1 Example 4 30% 1,3 butylene glycol aqueous solution 135.7 Example 5 30% ethanol aqueous solution 159.5

Example  6. Using a bath Five colors  Extract Manufacturing

Cut 30 g of loaves, 30 g of mustard, and 40 g of firewood to a certain size, and put 500 g of a mixture of Schisandra chinensis 60g, 60g, 60g, 60g, Gilgyeong 70g, Deodeok 50g, Seogtaetae 50g, and 50g black sesame seed in hemp cloth, 1500g of purified water, 250g of ethanol and 750 g of 1,3 butylene glycol was mixed with 2500 g of a mixed solvent, and extracted by boiling at 100 ° C. for 4 hours in an extractor equipped with a cooling condenser. Thereafter, the filtrate was filtered through a 300 mesh filter, and the mixture was left to stand for one week, and the precipitate was filtered through a 0.45 um filter. Then, the resultant was concentrated completely at 50 ° C. using a vacuum concentrator to obtain a dry weight of 115.6 g.

Example  7 ~ 10. Using bath water and various extraction solvents Five Bangs  Vigorous extract manufacture

Extracting the five generations by the same method as Example 6 using the solvent of Table 2 below and the results are shown in Table 2.

menstruum Yield (g) Example 7 water 73.7 Example 8 ethanol 117.5 Example 9 30% 1,3 butylene glycol aqueous solution 86.2 Example 10 30% ethanol aqueous solution 109.5

Example  11.: Skin lotion manufacture

1 kg of the lotion (skin lotion) in the cosmetic preparation containing the five-prong extract powder of Example 1 was prepared according to the contents of the following table and the following preparation method.

number Raw material Content (% by weight) One  Five generations extract powder (Example 1) 3.0 2  glycerin 3.0 3  Butylene Glycol 2.0 4  Propylene glycol 2.0 5  Polyoxyethylene Cured Castor Oil 1.0 6  ethanol 10.0 7  Triethanolamine 0.1 8  antiseptic a very small amount 9  Pigment a very small amount 10  Spices a very small amount 11  Purified water Remaining amount

<Production method>

11, 2, 3, 4, and 8 were sequentially added, stirred, and dissolved. Then, 5 was heated to 60 ° C. to dissolve, and 10 times were added to dissolve, followed by 11 times. Thereafter, 1, 6, 7, and 9 were added to No. 11, sufficiently stirred at room temperature, and aged for 3 days.

Example  12.: Nutritional Lotion

1 kg of nutrient lotion in the cosmetic preparation containing the five-pronged extract of Example 1 was prepared according to the content of the following table and the following preparation method.

number Raw material Content (% by weight) One  Five generations extract powder (Example 1) 3.0 2  Beeswax 1.0 3  Polysorbate 60 1.5 4  Solbitan Sesquioleate 0.5 5  Floating paraffin 10.0 6  Sorbitan stearate 1.0 7  Lipophilic Monostearic Acid Glycerin 0.5 8  Stearic acid 1.5 9 Glyceryl Stearate / Pig-400 Stearate 1.0 10 Propylene glycol 3.0 11 Carboxy Polymer 0.1 12 Triethanolamine 0.2 13 antiseptic a very small amount 14 Pigment a very small amount 15 Spices a very small amount 16  Purified water Remaining amount

<Production method>

10, 11, 13, and 16 were mixed and stirred to be heated to 80 ~ 85 ℃, put into the manufacturing unit, and then actuated emulsifier and 2, 3, 4, 5, 6, 7, 8, 9, 12 was 80 ~ 85 ℃ After dissolving by heating and emulsifying. After emulsification, cool down to 50 ℃ while stirring with a stirrer, add 15 times, cool to 45 ℃, add 14 times, add 1 to 35 ℃, cool to 25 ℃, and mature at room temperature for 3 days. I was.

Example  13: Preparation of nourishing cream

1 kg of nourishing cream in the cosmetic composition containing the five-pronged extract of Example 1 was prepared by the content of the following table and the following preparation method.

number Raw material Content (% by weight) One Five generations extract powder (Example 1) 3.0 2 Stearic acid 2.0 3 Cetyl alcohol 2.0 4 Glyceryl Monostearate 2.0 5 Polyoxyethylene sorbitan monostearate 0.5 6 Sorbitan sesquioleate 0.5 7 Glyceryl Monostearate / Glyceryl Stearate / Polyoxyethylene Stearate 1.0 8 Wax 1.0 9 Liquid paraffin 4.0 10 Squalane 4.0 11 Caprylic / Capric Triglycerides 4.0 12 Carboxy Vinyl Polymer 0.3 13 Butylene glycol 5.0 14 glycerin 3.0 15 Triethanolamine 0.5 16 Purified water Remaining amount

<Production method>

While stirring 12, 13, 14, and 16, and heating it between 80 ~ 85 ℃ put into the manufacturing unit and then actuated emulsifier and 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 80 ~ After heating to 85 ° C. to dissolve, 15 was added and stirred, and added to the production part, followed by emulsification. After emulsification, the mixture was cooled to 35 ° C. while stirring using a stirrer, and added 1 times, cooled to 25 ° C., and aged for 3 days.

Example  14. Essence Manufacturer

1 kg of the essence in the cosmetic composition containing the five-pronged extract of Example 1 was prepared by the following table and the following preparation method.

number        Raw material Content (% by weight) One  Five generations extract powder (Example 1) 3.0 2 Cytostolol 1.7 3  Polyglyceryl 2-oleate 1.5 4  Ceramide 0.7 5 Steares-4 1.2 6 cholesterol 1.5 7 Dicetylphosphate 0.4 8 Concentrated glycerin 5.0 9 Macadamia oil 15.0 10 Carboxy Vinyl Polymer 0.2 11  Xanthan Gum 0.2 12  antiseptic a very small amount 13  Spices a very small amount 14  Purified water Remaining amount

<Production method>

Nonionic amphiphilic lipids were prepared by homogenizing 2, 3, 4, 5 and 6 at 70-75 ° C. The nonionic amphiphilic lipids are mixed with 1, 7, 8, and 14, homogenized at 70-75 ° C., passed through a microfluidizer, and then homogenized by slowly adding 9 at 60-65 ° C., followed by microflu again. Passed it through again. Thereafter, 10, 11, 12, and 13 were added to disperse and stabilized, and aged at room temperature for 3 days.

Comparative Example

Nutritional creams were prepared using the ingredients and preparation described in Example 13, except that water was used instead of the five-pronged extract powder among the components of the table described in Example 13.

Experimental Example  One.

The cell proliferation effect using the cell culture was measured for the extract of Obangsaengdan dry powder prepared in Examples 1 to 10.

1) Experiment Method

Cytotoxicity and proliferation experiments were carried out in the following manner. Incubate the cells (fibroblast, 3T3) in 96-well microplates at 5,000 cells / well and incubate in a thermostat for 30 minutes, and sample is 0.01, 0.05, 0.1, 0.5, 1.0 (%, W / V) for each concentration. )) And incubated for 72 hours. Thiazoline blue was administered and further cultured for 4 hours. All cultures were discarded, the reaction stopper was added to each well of the microplate, and stirred for 5 minutes, and then absorbance was measured at 570 nm. As the control group, 10% Fetal Bovine Serum (FBS) medium was used as the sample injection amount to co-culture the optimal conditions for cell growth. .

2) Experiment result

The cell proliferation effect was calculated by Formula 1, and the results are shown in Table 3 below, and are plotted in the graph of FIG. 1.

[Calculation 1]

Concentration (%) Cell proliferation effect (%) Example One 2 3 4 5 6 7 8 9 10 Control 0 0 0 0 0 0 0 0 0 0 0.01 6.1 5.1 4.2 4.8 5.8 4.2 4.7 3.8 4.1 4.9 0.05 10.5 9.0 9.6 8.3 11.3 8.6 6.6 8.2 7.3 9.4 0.1 17.8 15.2 15.4 13.1 16.7 12.7 9.8 7.2 7.3 13.2 0.5 24.7 22.3 26.5 22.2 28.6 18.5 16.7 18.2 16.1 19.5 One 38.1 30.4 33.2 28.3 36.4 27.1 23.7 26.4 23.4 28.7

As shown in Table 3, when the cell proliferation in the optimal conditions for cell growth to 100%, the five-progeny extract produced in Examples 1 to 10 was confirmed that there is no cytotoxicity and has a cell proliferation effect. In particular, Examples 1 to 5 by the ultrasonic extraction method had a better cell proliferation effect than Examples 6 to 10 by the bath extraction method.

Experimental Example  2.

Free Radical scavenging effect was measured on the extract powders of the five stages prepared in Examples 1 to 10, and Quercetin was used as a comparative material.

1) Experiment Method

Experiments were carried out using the DPPH (1,1-diphenyl-2picryl-hydrazyl) method (Blois. MS Nature 181, 1190, 1958), and DPPH and Quercetin were used by SIGMA. . To 1 ml of 0.2 mM DPPH methanol solution, 2 ml of ethanol or methanol solution were added to Quercetin extracts of the Examples 1 to 10 and the control substance Quercetin in various concentrations (5, 10, 20, 30 ppm) and stirred well at room temperature. The reaction was carried out for a minute. The absorbance of each sample was measured at 517 nm, and purified water was used instead of each sample in the blank test.

The free radical scavenging effect was calculated using the following Equation 2, and the results are shown in Table 4 below, and the values are shown in FIG.

[Calculation 2]

2) Experiment result

Concentration (ppm) Free radical scavenging effect (%) Example Quercetin One 2 3 4 5 6 7 8 9 10 0 0 0 0 0 0 0 0 0 0 0 0 5 23.8 14.7 19.4 17.6 23.7 10.7 6.3 9.7 12.3 15.7 29.8 10 48.9 41.5 47.3 46.2 53.6 25.8 21.3 23.6 28.6 33.6 52.7 20 93.5 83.1 90.6 88.5 96.8 62.9 59.5 64.7 67.4 68.3 98.2 30 - 94.8 - 96.7 - 95.1 91.3 94.5 97.1 97.9 - 50% inhibitory concentration SC ₅₀ 10.6 ppm 13.9 ppm 11.1 ppm 13.1 ppm 10.1 ppm 16.4 ppm 17.5 ppm 16.5 ppm 15.7 ppm 15.1 ppm 9.7 ppm

As can be seen in Table 4, the free radical 50% scavenging concentration (SC ₅₀ ) of the _five generations of the present invention was found to be similar to the value of Quercetin. In addition, it was found that the free radical scavenging effect of the extracts of Example 1 to 5 extracted by the ultrasonic extraction method was superior to the free radical scavenging effect of the extract produced by the extracts of Examples 6 to 10 extracted by the boiling water extraction method.

Experimental Example  3.

The inhibitory effect of lipoxygenase (Lpoxygenase) activity was measured on the extracts of the five-produced seedlings prepared in Examples 1 to 10, and Quercetin was used as a comparative material.

1) Experiment Method

The experiment was performed using the TBAS method, and linoleic acid, lipoxygenase, thiobabitulic acid, and quercetin used for the experiment were used by SIGMA. To 1 ml of 1 mM linoleic acid, 0.05 ml of various concentrations (5, 10, 20, 30, 40 ppm) of Examples 1 to 10 and the control compound Quercetin were added, 0.95 ml of lipoxygenase was added, stirred well, and stirred at 25 ° C. for 10 minutes. The reaction was carried out. Then, 0.5 ml of trichloroacetic acid is administered, 1 ml of thiobarbiturlic acid is added, the mixture is heated at 95 ° C. for 10 minutes, and then cooled in ice water for 2-3 minutes to terminate the reaction. Then, 2 ml of butanol (Butanol) was added to the reaction solution, and centrifuged at 4000 g for 5 minutes, and the absorbance was measured at 535 nm. At this time, purified water was used instead of each sample.

To calculate the effect of inhibiting lipoxygenase activity using the following equation 3 and the results are shown in Table 5 below, it is shown in FIG.

[Calculation 3]

2) Experiment result

Concentration (ppm) Inhibitory effect of lipoxygenase activity (%) Example Quercetin One 2 3 4 5 6 7 8 9 10 0 0 0 0 0 0 0 0 0 0 0 0 5 23.5 17.3 22.3 13.6 19.5 9.2 6.6 8.4 7.3 10.3 25.4 10 51.8 43.2 52.3 38.9 44.8 16.2 11.5 15.7 17.1 20.7 63.9 20 85.9 78.5 88.2 79.3 83.7 44.7 40.7 42.3 41.8 50.9 97.8 30 95.7 91.9 98.3 90.7 96.2 82.5 77.9 80.1 83.5 88.6 - Lipoxygenase 50% inhibitory concentration (IC ₅₀ ) 12.6 ppm 14.2 ppm 12.3 ppm 14.7 ppm 13.3 ppm 21.0 ppm 22.4 ppm 21.6 ppm 21.0 ppm 21.0 ppm 9.4 ppm

As can be seen in Table 5, the inhibitory effect of lipoxygenase activity of the five-pronged extracts of Example 1 to 5 prepared by ultrasonic extraction method was similar to that of Quercetin lipoxygenase 50% activity inhibitory concentration (IC ₅₀ ). And it was found. In addition, it was confirmed that the effect of inhibiting the lipoxygenase activity of the five-season extracts produced by the ultrasonic extraction method than the lipoxygenase activity-inhibitory effect of the extracts of Examples 6-10 prepared by the bath extraction method. Could.

Experimental Example  4.

The inhibitory effect of hyaluronidase activity was measured on the extracts of the offspring produced in the present stages of Examples 1 to 10.

1) Experiment Method

It is a method of applying Morgan-Elson method. The final enzymatic activity of hyaluronidase was 400NF unit / ml and the final concentration of HA (Hyaluronic acid) was 0.4mg / ml, and the inactivation was performed using Compound 48/80 (Sigma C2313) buffer (0.1mg / ml) as an active agent. Hyaluronidase activity was measured centering on the inhibitory action of the activation phase of the type hyaluronidase. The sample was dissolved in a buffer to prepare a sample solution, and the control group used a buffer instead of the sample solution. In addition, buffers were used instead of enzyme solutions for each blank, and green tea extract was used as a control.

The following formula 4 was used to determine the effect of inhibiting hyaluronidase activity, and the results are shown in Table 6 below, which is shown in FIG.

[Calculation 4]

Inhibitory effect of hyaluronidase activity (%)

= 100- (response absorbance of each sample / response absorbance of control) * 100

2) Experiment result

Hyaluronidase activity inhibitory effect (%) Concentration (%) Example Green tea extract One 2 3 4 5 6 7 8 9 10 0 0 0 0 0 0 0 0 0 0 0 0 0.5 15.2 13.6 16.7 12.8 15.3 11.6 8.8 12.6 8.5 13.3 9.5 One 38.6 30.8 37.2 28.1 34.5 28.7 24.2 30.2 25.7 32.6 20.5 3 62.7 57.7 63.6 56.6 60.9 50.3 45.5 55.3 48.2 58.6 42.9 5 96.1 90.5 95.8 91.1 92.7 88.9 85.7 91.2 87.4 92.1 87.3 Hyaluronidase 50% Inhibitory Concentration (IC ₅₀ ) 2.3% 2.6% 2.3% 2.6% 2.4% 2.7% 2.9% 2.6% 2.9% 2.5% 3.0%

As can be seen in Table 6, the hyaluronidase activity inhibitory effect of the five-produced Ginseng extract of Example 1 to 10 prepared by the ultrasonic extraction method of the present invention is superior to the hyaluronidase activity inhibitory effect of the green tea extract as a control material It could be confirmed that.

Experimental Example  5.

Clinical evaluation of the atopic dermatitis improvement effect of the present invention of the peninsula progeny extract was measured.

In order to evaluate the improvement effect of the atopic skin of the five-season extract of the present invention, the nutrition cream prepared in Example 13 and Comparative Example (in Example 13 prepared by putting water instead of the five-season extract base powder of Example 1 The following experiment was conducted on 20 children who were treated with atopic dermatitis using a nutritional cream). The test sample was applied to the whole body by dividing the right side and the left side by the double-blinded test, but the use of other moisturizers that could affect the effect of the test sample was prohibited as much as possible. The effects after 1, 2, 3, and 4 weeks after the application of the test samples were evaluated by the SCORAD SCORAD: SCORing Atopic Dermatitis (AM) method, and the results are shown in Table 7 below.

1) Criteria

① Extent criteria: Area = damage area / 100

② intensity criteria

   -Erythema (1/2/3)

   -Edema (1/2/3)

   -Exudate (1/2/3)

   -Skin peeling (1/2/3)

   Taekwondo degree (1/2/3)

   -Drying degree (1/2/3)

Subjective criteria

   -Itching (1-10)

   Insomnia (1-10)

* Calculation of coderad = (degree of criterion / 5) + (strength criterion / 7) + subjective criterion

division Week 0 1 week 2 weeks 3 weeks 4 Weeks Example 13 Measures 66.5 40.2 22.5 10.7 8.3 % Improvement 0.00% 39.55% 66.17% 83.91% 87.52% Comparative Example Measures 65.3 46.7 25.8 17.6 11.5 % Improvement 0.00% 28.48% 60.49% 73.05% 82.39%

Referring to Table 7, the cosmetic composition of Example 13 containing the five-prototype extract of the present invention (Example 1) has an atopic skin improvement effect compared to the cosmetic preparation of Comparative Example prepared by adding water (Comparative Example 2) instead of the extract. It is very good.

In addition, the clinical results photographs before and after the use of cosmetics containing the five generations of the present invention, are shown in FIG.

On the other hand, in order to examine the atopic dermatitis mitigation effect felt by the caregiver, after using the cosmetics of Example 13 and Comparative Example for one month, the parents of the user according to the following criteria, itching, erythema change, dry skin and satisfaction with Together with the evaluation of the degree of improvement of the overall dermatitis and side effects by the test sample are shown in Table 8 below.

(Criteria)

One 2 3 4 5 no effect Some usually Great Very good Very dissatisfied dissatisfaction usually satisfied very good

Classification Itching of the skin Erythema change Dry skin Overall improvement Side Effect Example 13 4.5 4.3 3.7 4.5 4.4 Comparative Example 3.7 3.9 3.5 4.1 4.3

As shown in Table 8, the cosmetic composition of the present invention can be confirmed that there is an improvement effect in all the evaluation items for children with atopic skin as a test subject.

Figure 1 is a graph showing the cell proliferation effect of the five generations of the present invention.

Figure 2 is a graph showing the free radical scavenging effect of the five generations extract of the present invention.

Figure 3 is a graph showing the inhibitory effect of lipoxygenase activity of the five generations extract of the present invention

Figure 4 is a graph showing the inhibitory effect of hyaluronidase activity of the five generations extract of the present invention.

Figure 5 is a photograph of the clinical results before and after using the cosmetic composition containing the five generations of the present invention.

Claims (7)

Cosmetic composition containing extracts of five leaves of the genus five, mustard, firewood, Schisandra chinensis, chrysanthemum, turmeric, Gilkyung, deodeok, Seomoktae and black sesame. According to claim 1, 1-5% by weight of young leaves, 1-5% by weight of mustard, 3-7% by weight of firewood, 3-7% by weight of Schizandra chinensis, 5-10% by weight, 5-10% by weight of turmeric, 10 Cosmetic composition, characterized in that the mixture contained a dry weight ratio of ~ 15% by weight, Deodeok 5-10% by weight, Seomoktae 3-7% by weight, black sesame seed 3-7% by weight. The cosmetic composition according to claim 1, wherein the five-pronged base is extracted with at least one solvent selected from the group consisting of water, ethanol and 1,3 butylene glycol. According to claim 1, wherein the extracts of the five leaves of the five leaves, mustard, fire wood, Schisandra chinensis, lump, turmeric, Gilkyung, deodeok, Seomoktae and black sesame, the wavelength of the ultrasound is 35-45kHz, the output of 300-500watt Cosmetic composition that is extracted with. The cosmetic composition according to claim 1, wherein the extract is contained in an amount of 0.001 to 5% by weight based on the total dry weight of the cosmetic composition. The cosmetic composition according to claim 5, wherein the extract has an antioxidant, anti-inflammatory and atopic skin improvement effect. The cosmetic composition according to claim 5 or 6, wherein the cosmetic composition is softening cream, nutrient cream, nourishing cream, massage cream, essence or pack formulation.

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