CN111135123A - Preparation method and application of selaginella tamariscina fermentation extracting solution - Google Patents
Preparation method and application of selaginella tamariscina fermentation extracting solution Download PDFInfo
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- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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Abstract
The invention discloses a selaginella tamariscina fermentation extracting solution, a preparation method thereof and application thereof in skin-care cosmetics. The raw materials of the selaginella tamariscina fermentation extracting solution comprise a fermentation system and seed bacterial liquid, wherein the fermentation system consists of selaginella tamariscina, glucose, casein, vitamin C and water; the seed bacterial liquid comprises sodium acetate, soybean peptone, glucose, yeast extract, dipotassium hydrogen phosphate, magnesium sulfate, manganese sulfate, distilled water and lactobacillus buchneri. The preparation method comprises the following steps: respectively preparing seed bacterial liquid and a fermentation system; diluting the seed bacterial liquid, and then inoculating the diluted seed bacterial liquid into a fermentation system for standing and fermentation; filtering the obtained fermented product, separating liquid phase part from solid phase part, microfiltering the liquid phase part to remove thallus, adding polyalcohol, and adjusting pH to obtain herba Selaginellae fermented extract. The invention adopts natural plant raw materials and probiotics for fermentation, fully utilizes the degradation effect of the thallus metabolic process on macromolecular compounds to prepare the extracting solution, and has the effects of moisturizing, repairing substrates, regulating skin micro-ecology and the like.
Description
Technical Field
The invention relates to a method for extracting high-activity lysate from tamariskoid spikemoss herb through fermentation, in particular to a method for preparing extracting solution by fermenting tamariskoid spikemoss herb with probiotics, and belongs to the technical field of biology.
Background
The selaginella tamariscina is a soil-borne or stone-borne resuscitation plant, is in a whole-grass-path cushion shape, has the trehalose content of about 0.14 percent, has extremely strong drought resistance, can survive under the physiological condition of dehydration of more than 95 percent and can rapidly resuscitate when meeting water. The flavonoids in the whole plant have a content of about 0.42%, so the composition has the effects of stopping bleeding, astringing, resisting inflammation and repairing skin. The herba selaginellae dry leaves contain abundant natural skin care factors such as polysaccharide, amino acid, protein and flavonoid, and have strong moisturizing and antioxidant effects.
At present, the main content of the most patents related to the selaginella tamariscina is extraction of flavonoid compounds, a water extraction method is mostly adopted in the extraction method, in the extraction process, the selaginella tamariscina raw material is in a high-temperature state of more than 80 ℃ for a long time, the stability of the main active ingredients of the selaginella tamariscina is damaged, and meanwhile, a series of problems of high energy consumption, low raw material extraction rate, large wastewater discharge, poor smell of an extracting solution, poor color stability and the like exist. Through patent review, only patent CN 106726976A relates to an anti-dandruff and anti-oxygen fermented medicine, a preparation method and application thereof, the patent only adds a small amount of selaginella tamariscina in more than ten herbal raw materials, and adopts yeast fermentation, and does not relate to lactic acid bacteria fermentation in probiotics to prepare a selaginella tamariscina fermented extract.
At present, the research of extracting the nutrient of the selaginella tamariscina through fermentation is limited, and the research of the biological fermentation plant extract has great expansion space based on the increasing demand of natural cosmetic raw materials in the market.
Disclosure of Invention
The technical problem to be solved by the invention is as follows: a herba Selaginellae nutrition and its preparation method are provided.
In order to solve the technical problem, the invention provides a selaginella tamariscina fermentation extract which is characterized in that raw materials comprise a fermentation system and seed bacterial liquid, wherein the fermentation system consists of selaginella tamariscina, glucose, casein, vitamin C and water; the seed bacterial liquid comprises sodium acetate, soybean peptone, glucose, yeast extract, dipotassium hydrogen phosphate, magnesium sulfate, manganese sulfate, distilled water and lactobacillus buchneri.
Preferably, the weight percentage of the selaginella tamariscina in the fermentation system is 0.1-3%, the weight percentage of the glucose is 0.1-2%, the weight percentage of the casein is 0.001-0.04%, the weight percentage of the vitamin C is 0.001-0.01%, and the balance is water; the bacterial liquid comprises, by weight, 0.6 part of sodium acetate, 4 parts of soybean peptone, 4 parts of glucose, 1 part of yeast extract, 0.4 part of dipotassium hydrogen phosphate, 0.12 part of magnesium sulfate, 0.05 part of manganese sulfate and 200 parts of distilled water.
Preferably, the lactobacillus buchneri is deposited in the general microbiological center of the china microbiological culture collection administration (No. 1 sith west way in north zhou sunward area, No. 3) in 2019, 12 months and 12 days, and the number is CGMCC No.19125, and the classification name is: lactobacillus buchneri (Lactobacillus buchneri).
Preferably, the seed bacterial liquid is diluted and quantified to 1 × 109cfu/mL is added into the fermentation system, and the adding amount is 0.5-3% of the mass of the fermentation system.
The invention also provides a preparation method of the selaginella tamariscina fermentation extracting solution, which is characterized by comprising the following steps of:
step 1): mixing sodium acetate, soybean peptone, glucose, yeast extract, dipotassium hydrogen phosphate, magnesium sulfate, manganese sulfate and distilled water uniformly, adjusting the pH value to 6.2 +/-0.2, sterilizing by steam, and cooling to room temperature;
step 2): inoculating lactobacillus buchneri to the mixed liquid obtained in the step 1) for activated culture to prepare seed bacterial liquid;
step 3): mixing herba Selaginellae powder with glucose, casein, vitamin C and water, steam sterilizing, and cooling to room temperature to obtain fermentation system;
step 4): diluting the seed bacterial liquid, and then inoculating the diluted seed bacterial liquid into a fermentation system for standing and fermentation;
step 5): filtering the obtained fermented product, separating liquid phase part from solid phase part, microfiltering the liquid phase part to remove thallus, adding polyalcohol, and adjusting pH to 5.0-6.0 to obtain herba Selaginellae fermented extract.
Preferably, the temperature of the steam sterilization in the step 1) and the step 3) is 121 ℃ and the time is 15 min.
Preferably, the time for the activation culture in the step 2) is 48 h.
Preferably, the preparation method of the selaginella tamariscina powder in the step 3) comprises the following steps: selecting dried and dehydrated herba Selaginellae, removing root and mud, selecting herba Selaginellae leaves, cleaning, oven drying, and pulverizing to 60-100 mesh to obtain herba Selaginellae powder.
Preferably, the fermentation temperature in the step 4) is 37 ℃, and the fermentation period is 24h to 7 days.
Preferably, the pore size of the filter membrane used for filtering in the step 5) is 40 μm; the pore of the filter membrane used for microfiltration is 0.22 μm; the addition amount of the liquid phase part of the polyhydric alcohol is 5.1 to 5.6 percent of the mass of the liquid phase part; the solid phase part adopts ultrasonic high-speed shearing to break the thalli, and is made into a mud film after homogenization.
The invention also provides application of the tamariskoid spikemoss herb fermented extracting solution in skin-care cosmetics, which is characterized in that the adding amount of the tamariskoid spikemoss herb fermented extracting solution is 10-20% of the total mass of all the raw materials.
The invention provides a preparation method of selaginella fermented extract, which adopts natural plant raw materials and probiotics for fermentation, fully utilizes the degradation effect of a thallus metabolic process on macromolecular compounds to prepare the extract, has the effects of moisturizing, repairing substrates, regulating skin micro-ecology and the like, and can be widely applied to skin-care cosmetics.
According to the invention, lactobacillus buchneri is adopted as the lactobacillus, and through a control experiment with an extraction method, the strain can more efficiently dissolve out active compounds of selaginella tamariscina, so that the contents of flavone, polysaccharide, amino acid, protein and the like are obviously improved, and meanwhile, the strain has good low immunogenicity. Secondly, the fermenting filtrate of the fermented herba selaginellae of the lactobacillus buchneri CGMCC No.19125 keeps natural pleasant smell of the plant source herbs, and mutual gain of the plant source and the probiotics is realized. Thirdly, the separation and sterilization processes of the production all adopt a normal temperature treatment mode, the loss and the damage of the selaginella tamariscina activity factor are greatly reduced, and in addition, compared with an extraction method, the process also greatly reduces the energy consumption. Fourthly, in the fermentation process of the lactobacillus buchneri CGMCC No.19125 selaginella tamariscina provided by the invention, on one hand, the fermentation sterilizing filtrate can be used for natural raw materials of cream, moisturizing water, essence and the like of high-end cosmetics, so that the moisturizing, anti-inflammatory and anti-allergy functions of the cosmetics are enhanced, and the skin is more exquisite, moist and active. On the other hand, the fermented filter residue is cut and broken by cells to obtain the mixed lysate of selaginella tamariscina and lactobacillus, and the mixed lysate is further prepared into a mud film which can be used as a raw material of a natural facial mask and the like. In conclusion, the process technology for preparing the fermentation extracting solution by fermenting the selaginella tamariscina with the lactobacillus buchneri CGMCC No.19125 provided by the invention has the advantages that no waste water and waste residues are discharged in the process, meanwhile, the production process adopts strict anaerobic fermentation control, basically no waste gas is discharged, and the green and environment-friendly effects of the production technology are effectively realized.
Compared with the prior art, the invention has remarkable technical progress. The method is different from the traditional water extraction method in the extraction by using a microbiological method, promotes the dissolution of the high-activity compounds of the selaginella tamariscina by using the metabolic action of the lactobacillus, and improves the effects of moisturizing, anti-inflammation and repairing of the extracting solution. The natural pleasant smell of the selaginella tamariscina is greatly reserved by adopting a non-high-temperature sterilization process, and the concept of green skin care is realized by establishing skin micro-ecology.
The invention provides a process for dissolving out and enhancing the effect of beneficial lactic acid bacteria on active compounds of selaginella tamariscina, the obtained fermentation extracting solution is pleasant in smell, is rich in various high-activity factors such as flavone, polysaccharide, amino acid and protein, can be quickly absorbed and utilized by skin, can be widely applied to moisturizing water, skin cream and essence, and achieves the natural skin care effects of long-acting moisturizing, anti-inflammation and anti-allergy, substrate repairing and epidermal flora balancing.
Detailed Description
In order to make the invention more comprehensible, preferred embodiments are described in detail below.
The lactobacillus buchneri used in the following examples was deposited in the general microbiological culture collection center of the china microbiological culture collection administration committee (No. 1 west way, No. 3, north beijing, south china, korean) on 12 months and 12 days in 2019, with the number of cgmccno.19125, classification name: lactobacillus buchneri (Lactobacillus buchneri).
Example 1
A method for preparing herba Selaginellae fermented extract comprises:
1) activation of fermentation Strain
Weighing 0.6g of sodium acetate, 4g of soybean peptone, 4g of glucose, 1g of yeast extract, 0.4g of dipotassium hydrogen phosphate, 0.12g of magnesium sulfate, 0.05g of manganese sulfate and 200ml of distilled water, adjusting the pH value to 6.2 +/-0.2, sterilizing by high-pressure steam for 15min, cooling to room temperature, inoculating a preservation strain Lactobacillus buchneri CGMCC No.19125, and performing activated culture for 48h to obtain an activated seed culture medium.
2) Preparation of fermentation Medium
Taking the whole dried and dehydrated selaginella tamariscina, cleaning, drying, collecting the dried leaves of the selaginella tamariscina, and crushing to obtain 60-mesh selaginella tamariscina powder. Adding 0.1 wt% herba Selaginellae powder, 0.1 wt% glucose, 0.001 wt% casein, 0.001 wt% vitamin C, and water for the rest, shaking thoroughly, and sterilizing at 121 deg.C for 15 min.
3) Fermentation process
The seed culture medium is activated and diluted to 1 × 109cfu/mL seed bacterial liquid. Adding seed bacteria liquid with the mass of 0.5 wt% of the mass of the fermentation system into the fermentation medium, and fermenting for 24h at 37 ℃.
4) Post-fermentation treatment
After fermentation, removing plant residue from the supernatant with filter membrane with pore diameter of 40 μm, removing thallus with filter membrane with pore diameter of 0.22 μm, and adjusting pH to 5.0-6.0 to obtain herba Selaginellae fermented extractive solution; the solid phase part is ultrasonically crushed for 1 hour and homogenized for 1.5 hours to prepare a mud film.
Example 2
A method for preparing herba Selaginellae fermented extract comprises:
1) activation of fermentation Strain
Weighing 0.6g of sodium acetate, 4g of soybean peptone, 4g of glucose, 1g of yeast extract, 0.4g of dipotassium hydrogen phosphate, 0.12g of magnesium sulfate, 0.05g of manganese sulfate and 200ml of distilled water, adjusting the pH value to 6.2 +/-0.2, sterilizing by high-pressure steam for 15min, cooling to room temperature, inoculating a preservation strain Lactobacillus buchneri CGMCC No.19125, and performing activated culture for 48h to obtain an activated seed culture medium.
2) Preparation of fermentation Medium
Taking the whole dried and dehydrated selaginella tamariscina, cleaning, drying, collecting the dried leaves of the selaginella tamariscina, and crushing to obtain 80-mesh selaginella tamariscina powder. Adding 1 wt% herba Selaginellae powder, 1 wt% glucose, 0.02 wt% casein, 0.005 wt% vitamin C into the fermentation system, adding water for the rest, shaking thoroughly, and sterilizing at 121 deg.C for 15 min.
3) Fermentation process
The seed culture medium is activated and diluted to 1 × 109cfu/mL seed bacterial liquid. Adding seed bacteria liquid with the mass of 2 wt% of the mass of the fermentation system into the fermentation medium, and fermenting for 3 days at 37 ℃.
4) Post-fermentation treatment
After fermentation, removing plant residue from the supernatant with filter membrane with pore diameter of 40 μm, removing thallus with filter membrane with pore diameter of 0.22 μm, and adjusting pH to 5.0-6.0 to obtain herba Selaginellae fermented extractive solution; the solid phase part is ultrasonically crushed for 1 hour and homogenized for 1.5 hours to prepare a mud film.
Example 3
A method for preparing herba Selaginellae fermented extract comprises:
1) activation of fermentation Strain
Weighing 0.6g of sodium acetate, 4g of soybean peptone, 4g of glucose, 1g of yeast extract, 0.4g of dipotassium hydrogen phosphate, 0.12g of magnesium sulfate, 0.05g of manganese sulfate and 200ml of distilled water, adjusting the pH value to 6.2 +/-0.2, sterilizing by high-pressure steam for 15min, cooling to room temperature, inoculating a preservation strain Lactobacillus buchneri CGMCC No.19125, and performing activated culture for 48h to obtain an activated seed culture medium.
2) Preparation of fermentation Medium
Taking the whole dried and dehydrated selaginella tamariscina, cleaning, drying, collecting the dried leaves of the selaginella tamariscina, and crushing to obtain 100-mesh selaginella tamariscina powder. Adding herba Selaginellae 3 wt%, glucose 2 wt%, casein 0.04 wt%, vitamin C0.01 wt%, and water in balance, shaking thoroughly, and sterilizing at 121 deg.C for 15 min.
3) Fermentation process
The seed culture medium is activated and diluted to 1 × 109cfu/mL seed bacterial liquid. Adding 3 wt% of seed bacterial liquid into the fermentation medium, and fermenting in a 37 deg.C incubator for 7 days.
4) Post-fermentation treatment
After fermentation, removing plant residue from the supernatant with filter membrane with pore diameter of 40 μm, removing thallus with filter membrane with pore diameter of 0.22 μm, and adjusting pH to 5.0-6.0 to obtain herba Selaginellae fermented extractive solution; the solid phase part is ultrasonically crushed for 1 hour and homogenized for 1.5 hours to prepare a mud film.
Comparative example 1
Taking the whole dried and dehydrated selaginella tamariscina, cleaning, drying, collecting the dried leaves of the selaginella tamariscina, and crushing to obtain 100-mesh selaginella tamariscina powder. Weighing herba Selaginellae powder 3 wt% of the water extraction system, adding water to balance, and refluxing at 100 deg.C for 3 hr. After extraction, removing plant residue from the supernatant with filter membrane with pore diameter of 0.22 μm, and adjusting pH to 5.0-6.0 to obtain herba Selaginellae extract.
The fermentation extracts prepared in examples 1 to 3 and comparative example 1 were subjected to relevant tests.
The detection method comprises the following steps:
1. concentration of flavone
1) Standard solution preparation
Precisely weighing 0.06g of rutin control, adding an appropriate amount of 60% methanol, heating and dissolving in water bath, cooling, dissolving in 100mL volumetric flask with 60% methanol, shaking up for use, and preparing into standard solution with rutin content of 0.6g/L for use.
2) Preparation of Standard Curve
Adding 2.5mL of standard solution with different dilution times, and adding 400uL of 5 wt% NaNO2Standing for 5min, adding 400uL10 wt% Al (NO)3)3Standing for 6min, adding 600uL 10 wt% NaOH, standing for 6min, diluting with 30% ethanol to 10mL, standing for 10min, measuring light absorption at 510nmAnd (4) degree.
3) Detection of a sample to be tested
Changing the standard liquid in the step 2) into the liquid to be detected.
2. Amino acid content
Reference QB/T2409-
3. Polysaccharide content
Reference SN/T4260-
4. Protein content
Reference GB/T6432-2018
Specific data of the above test are shown in table 1.
TABLE 1
Example 4
The blue-light-resistant and anti-allergy skin lotion comprises the following components in percentage by mass:
| composition (I) | Content (wt.) |
| Water (W) | 80wt％ |
| Fermented extract of herba Selaginellae | 10wt％ |
| Glycerol | 3％ |
| Butanediol | 2％ |
| P-hydroxyacetophenone | 0.5％ |
| Pentanediol | 0.5％ |
| Citric acid sodium salt | 0.3％ |
| Hyaluronic acid | 0.1％ |
| Ethyl hexyl glycerol | 0.1％ |
The preparation method comprises the following steps: adding water, glycerol, butanediol, p-hydroxyacetophenone, pentanediol, sodium citrate and hyaluronic acid into a water phase pot, heating to 80 ℃, preserving heat, stirring and dissolving uniformly; cooling to 40 deg.C, adding herba Selaginellae fermented extractive solution, and stirring to obtain blue light resisting and antiallergic skin caring liquid.
Example 5
The antioxidant moisturizing skin care lotion comprises the following components in percentage by mass:
the preparation method comprises the following steps: adding water, glycerol, butanediol, water-soluble vitamin E, resveratrol, sodium citrate and hyaluronic acid into a water phase pot, heating to 80 ℃, preserving heat, stirring and dissolving uniformly; cooling to 40 deg.C, adding herba Selaginellae fermented extractive solution, and stirring to obtain blue light resisting and antiallergic skin caring liquid.
Example 6
An antioxidant moisturizing skin cream comprises the following components in percentage by mass:
| composition (I) | Content (wt.) |
| Monoglyceride | 2wt％ |
| Spermaceti stearic acid | 2wt％ |
| Plant squalane | 2.4％ |
| Olive oil | 3％ |
| Shea butter | 2.8％ |
| Plant sterol | 1％ |
| Fermented extract of herba Selaginellae | 16.8％ |
| Phenoxyethanol | 0.5％ |
| Water (W) | 70％ |
The preparation method comprises the following steps: adding monoglyceride, cetostearyl alcohol, plant squalane, olive oil, shea butter, phytosterol, etc. into an oil phase pot, heating to 80-85 deg.C, adding water, humectant such as herba Selaginellae fermentation extract, etc. into a water phase pot, heating to 80-85 deg.C, maintaining constant temperature for 30min, simultaneously pumping the raw materials in the oil phase pot and the water phase pot into a main emulsifying pot for emulsifying, stirring at 25rpm, homogenizing for 5-8min at 3600 rpm, cooling to 40 + -5 deg.C, stirring uniformly, mixing, and discharging at a temperature below 30 deg.C.
Comparative example 2
The plant moisturizing skin care lotion comprises the following components in percentage by mass:
the preparation method comprises the following steps: adding water, glycerol, butanediol, water soluble vitamin E, resveratrol, sodium citrate, and hyaluronic acid into water phase pot, heating to 80 deg.C, maintaining the temperature, stirring, and dissolving. Cooling to 40 deg.C, adding herba Selaginellae fermented extractive solution, and stirring to obtain plant moisturizing skin care lotion.
The skin lotions prepared in examples 4 to 6 and comparative example 2 were subjected to the related tests.
The detection method comprises the following steps:
1. hyaluronidase inhibition:
preparing a reagent:
preparation method of acetylacetone reagent: 26.5g of Na were weighed2C03Water was added to a volume of 500mL, 4.9mL of acetylacetone and 45mL of water were added, and the pH was adjusted to 9.8 with HCI.
The preparation method of the benzaldehyde reagent comprises the following steps: 1.6g of p-dimethylaminobenzaldehyde is weighed and dissolved in a mixed solution of 60mL of absolute ethyl alcohol and 60mL of concentrated hydrochloric acid.
2. The lipoxygenase detection method comprises the following steps:
preparing a reagent:
LOX enzyme: 1 volume of 5-LOX enzyme plus 50 volumes of distilled water.
Substrate: tween 20 is dispersed in 0.2moL/L boric acid buffer solution at the concentration of 0.5mL/L, linoleic acid (the purity of linoleic acid is more than 99%) is added at the concentration of 0.54mL/L, and after uniform mixing, the pH value of the buffer solution is adjusted to 9.0 by 1moL/L sodium hydroxide.
Specific data of the above test are shown in table 2.
TABLE 2
Claims (11)
1. A herba Selaginellae fermentation extract is characterized in that the raw materials comprise a fermentation system consisting of herba Selaginellae, glucose, casein, vitamin C and water, and seed bacteria liquid; the seed bacterial liquid comprises sodium acetate, soybean peptone, glucose, yeast extract, dipotassium hydrogen phosphate, magnesium sulfate, manganese sulfate, distilled water and lactobacillus buchneri.
2. The fermented extract of tamariskoid spikemoss herb according to claim 1, wherein the fermented extract of tamariskoid spikemoss herb is 0.1-3 wt%, glucose is 0.1-2 wt%, casein is 0.001-0.04 wt%, vitamin C is 0.001-0.01 wt%, and water is the rest; the bacterial liquid comprises, by weight, 0.6 part of sodium acetate, 4 parts of soybean peptone, 4 parts of glucose, 1 part of yeast extract, 0.4 part of dipotassium hydrogen phosphate, 0.12 part of magnesium sulfate, 0.05 part of manganese sulfate and 200 parts of distilled water.
3. The fermented extract of selaginella tamariscina according to claim 1 or 2, wherein said lactobacillus buchneri is deposited in the general microbiological center of the china committee for culture collection of microorganisms (No. 3 western road No.1, north china, south china, tokyo, township, No. 3) in 12 months and 12 days in 2019, with the number of CGMCC No.19125, classification name: lactobacillus buchneri (Lactobacillus buchneri).
4. The fermented extract of Selaginella tamariscina according to claim 1, wherein the amount of diluted seed suspension is 1X 109cfu/mL is added into the fermentation system, and the adding amount is 0.5-3% of the mass of the fermentation system.
5. The method for preparing fermented extract of tamariskoid spikemoss herb according to any of claims 1 to 4, comprising the steps of:
step 1): mixing sodium acetate, soybean peptone, glucose, yeast extract, dipotassium hydrogen phosphate, magnesium sulfate, manganese sulfate and distilled water uniformly, adjusting the pH value to 6.2 +/-0.2, sterilizing by steam, and cooling to room temperature;
step 2): inoculating lactobacillus buchneri to the mixed liquid obtained in the step 1) for activated culture to prepare seed bacterial liquid;
step 3): mixing herba Selaginellae powder with glucose, casein, vitamin C and water, steam sterilizing, and cooling to room temperature to obtain fermentation system;
step 4): diluting the seed bacterial liquid, and then inoculating the diluted seed bacterial liquid into a fermentation system for standing and fermentation;
step 5): filtering the obtained fermented product, separating liquid phase part from solid phase part, microfiltering the liquid phase part to remove thallus, adding polyalcohol, and adjusting pH to 5.0-6.0 to obtain herba Selaginellae fermented extract.
6. The method for preparing fermented extract of herba Selaginellae as claimed in claim 5, wherein the steam sterilization in step 1) and step 3) is performed at 121 deg.C for 15 min.
7. The method for preparing fermented extract of Selaginella tamariscina according to claim 5, wherein the activation incubation time in step 2) is 48 hours.
8. The method for preparing fermented extract of herba Selaginellae as claimed in claim 5, wherein the method for preparing herba Selaginellae powder in step 3) comprises: selecting dried and dehydrated herba Selaginellae, removing root and mud, selecting herba Selaginellae leaves, cleaning, oven drying, and pulverizing to 60-100 mesh to obtain herba Selaginellae powder.
9. The method for preparing fermented extract of Selaginella tamariscina according to claim 5, wherein the fermentation temperature in step 4) is 37 ℃ and the fermentation period is 24h to 7 days.
10. The method for preparing fermented extract of Selaginella tamariscina according to claim 5, wherein the pores of the filter membrane used in the step 5) are 40 μm; the pore of the filter membrane used for microfiltration is 0.22 μm; the addition amount of the liquid phase part of the polyhydric alcohol is 5.1 to 5.6 percent of the mass of the liquid phase part; the solid phase part adopts ultrasonic high-speed shearing to break the thalli, and is made into a mud film after homogenization.
11. The use of fermented extract of tamariskoid spikemoss herb as claimed in any of claims 1 to 4 in skin care cosmetics, wherein the amount of fermented extract of tamariskoid spikemoss herb is 10 to 20% of the total mass of all the raw materials.
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