Whitening skin lightening water and preparation method thereof and tyrosinase inhibitor
Technical field
The present invention relates to a kind of whitening skin lightening water and preparation method thereof and tyrosinase inhibitors, belong to cosmetic field.
Background technique
Facial pigmentation deposition is the skin problem to receive much attention.Chloasma, freckle and melanin deposition will affect appearance,
To bring worry.Whitening additive can reduce black sediment or reduce skin coloration, and Chinese women is increasingly
Pale skin is pursued, so that the demand of whitening skin lightening water persistently rises.
Existing whitening additive in the prior art is organic synthesis object mostly, these substances are irritating to the skin effect,
Side effect is larger, increases the harmfulness of human body, safety coefficient reduces;In addition, also having using single plant extract as beauty
White additive, but the whitening composition of the plant extracts is single, the mechanism of action is also relatively simple, and white-skinned face function is unobvious.
Patent application document CN101904809A discloses a kind of lightening compositions, and the composition contains root bark of white mulberry extraction
Object, camomile extract, gadol extract, grape seed extract, licorice, chicken mulberry extract, arbutin, kojic acid two
Palmitinic acid enzyme, niacinamide, tetrahydro curcumin, moisturizer and preservative, the composition can inhibit tyrosinase to a certain extent
Activity inhibit melanin to be formed so that melanin be blocked to transport.But containing two palmitinic acid enzyme of organic compound kojic acid,
Niacinamide has certain harm to human body.
Summary of the invention
Problems to be solved by the invention
In view of the less effective of Chinese herbal medicine whitening spot-removing preparation in the prior art, the problems such as whitening effect is poor, this hair
It is bright to provide firstly a kind of whitening skin lightening water.Whitening skin lightening water of the invention is excellent to the inhibiting effect of tyrosinase activity, no
Whitening function can be only played, and without side-effects to human body.
Further, the tyrosinase inhibitor excellent the present invention also provides a kind of pair of tyrosinase activity inhibiting effect.
Further, the present invention also provides a kind of easy to operate, preparation methods for the whitening skin lightening water that raw material is easily obtained.
The solution to the problem
The present invention provides a kind of whitening skin lightening water comprising tyrosinase inhibitor;The tyrosinase inhibitor includes
Kudzu root extract and the careless extract of resurrection, in terms of the gross mass of the tyrosinase inhibitor, the addition of the kudzu root extract
Amount is 0.5-75%, and the additional amount for bringing back to life careless extract is 25-99.5%.
Whitening skin lightening water according to the present invention, wherein the kudzu root extract and the mass ratio for bringing back to life careless extract
For 1:0.4~90, preferably 1:1~80, more preferably 1:2~70, further preferably 1:2.5~60, still more preferably
For 1:3~55, much further preferably from 1:3.5~50.
Whitening skin lightening water according to the present invention, wherein in terms of the gross mass of the whitening skin lightening water, the tyrosinase suppression
The additional amount of preparation is 0.01-12%.
Whitening skin lightening water according to the present invention, wherein the whitening skin lightening water further include moisturizer, thickener, solubilizer,
Skin conditioning agent, Shu Min agent and preservative;Wherein, in terms of the gross mass of the whitening skin lightening water, the addition of the moisturizer
Amount is 0.01-20%;The additional amount of the thickener is 0.02-0.8%;The additional amount of the solubilizer is 0.01-0.5%;
The additional amount of the skin conditioning agent is 0.01-5%;The additional amount of the Shu Min agent is 0.01-5%;The preservative adds
Entering amount is 0.01-1.5%.
Whitening skin lightening water according to the present invention, wherein the moisturizer includes dipropylene glycol, dipropylene glycol, propylene glycol, fourth
The combination of one or more of glycol, panthenol, glycerol, polyethylene glycol -32, glycerin polyether -26, Sodium Hyaluronate;With/
Or
The thickener include acrylic acid (ester) class/C10-30 alkylol acrylamide acid esters cross-linked polymer, hydroxyethyl cellulose,
Xanthan gum, hydroxy-ethyl acrylate/sodium acryloyldimethyl taurate copolymers, carbomer and ammonium acryloyldime-thyltaurate/
One or more of VP copolymer.
Whitening skin lightening water according to the present invention, wherein the solubilizer includes polysorbate -20, PEG-40 hydrogenation castor
One or more of sesame oil and PPG-26- butylol polyether -26;And/or
The preservative include one or both of Phenoxyethanol, methyl hydroxybenzoate, Nipasol, benzoic acid and its salt with
On combination.
Whitening skin lightening water according to the present invention, wherein the skin conditioning agent includes bulk kelp extract, vitamin E, oat
Peptide, Cer NS, bladder-wrack extract, chlorella tunning, Hydrolyzed Collagen, hydrolyzed wheat protein, green alga are extracted
Object, brown alga extract, allantoin, Bacillus acidi lactici/fermented soybean product extract, Herba Visci leaf extract, rhizoma imperatae mention
Take the combination of one or more of object, Radix et Caulis Opuntiae Dillenii extract, trehalose;And/or
The Shu Min agent includes hamamelis water, camomile extract, purslane extract, Gotu Kola P.E, ginger
Root extract, bisabolol, the combination of one or more of dipotassium glycyrrhizinate.
The present invention also provides a kind of tyrosinase inhibitors comprising kudzu root extract and the careless extract of resurrection, with described
The gross mass meter of tyrosinase inhibitor, the additional amount of the kudzu root extract are 0.5-75%, the careless extract of resurrection
Additional amount is 25-99.5%.
Tyrosinase inhibitor according to the present invention, wherein the kudzu root extract and the matter for bringing back to life careless extract
Amount is than being 1:0.4~90, preferably 1:1~80, more preferably 1:2~70, further preferably 1:2.5~60, further
Preferably 1:3~55, much further preferably from 1:3.5~50.
The present invention also provides a kind of preparation methods of whitening skin lightening water according to the present invention comprising by the whitening skin lightening
The step of each component mixing of water.
The effect of invention
The formula of whitening skin lightening water of the invention is mild, can effectively inhibit the activity of tyrosinase, to play whitening
Effect.
Further, tyrosinase inhibitor of the invention is excellent to the active inhibiting effect of tyrosinase.
Further, the preparation method of whitening skin lightening water of the invention is easy to operate, and raw material is easily obtained, and can be criticized
Amount production.
Detailed description of the invention
Fig. 1 shows logarithm mass concentration-tyrosinase inhibition rate pass of the kudzu root extract of comparative example 1-5 of the present invention
System's figure;
Fig. 2 shows logarithm mass concentration-tyrosinase inhibition rates of the careless extract of the resurrection of comparative example 6-9 of the present invention
Relational graph;
Fig. 3 shows logarithm mass concentration-tyrosinase inhibition rate of the tyrosinase inhibitor of 1-7 of the embodiment of the present invention
Relational graph.
Fig. 4 shows the content and interaction of kudzu root extract in the tyrosinase inhibitor of 1-7 of the embodiment of the present invention
The relational graph of coefficient.
Fig. 5 shows the comparison diagram of the melanin change rate of Application Example 1-8 of the present invention and Comparison study example 1-2.
Specific embodiment
It will be detailed below various exemplary embodiments, feature and aspect of the invention.Dedicated word " example herein
Property " mean " being used as example, embodiment or illustrative ".Here as any embodiment illustrated by " exemplary " should not necessarily be construed as
Preferred or advantageous over other embodiments.
In addition, in order to better illustrate the present invention, numerous details is given in specific embodiment below.
It will be appreciated by those skilled in the art that without certain details, the present invention equally be can be implemented.In other example,
Method well known to those skilled in the art, means, equipment and step are not described in detail, in order to highlight master of the invention
Purport.
First embodiment
First embodiment of the invention provides a kind of tyrosinase inhibitor comprising tyrosinase inhibitor;It is described
Tyrosinase inhibitor includes kudzu root extract and the careless extract of resurrection.Tyrosinase inhibitor of the invention can be good at pressing down
The activity of tyrosinase processed, in cosmetics in use, in use, being imitated with excellent whitening especially in whitening skin lightening water
Fruit.
Pueraria lobata is legume pueraria lobata (Pueraria lobata (wild) Ohwi) or sweet kudzu (Pueraria
Thomsonil Benth) dry root, sweet kudzu practise again referred to as Pachyrhizua angulatus.China shares 11 kinds of Pueraria, except Pachyrhizua angulatus is with artificial
Outer based on cultivation, remaining is based on wild.Chinese Pharmacopoeia (nineteen ninety version) has recorded two kinds of Pueraria lobotas of elegant jessamine and Pachyrhizua angulatus and has belonged to former plant.
The main composition of pueraria lobata is: starch, cellulose, protein and general flavone, in addition also contains a small amount of fat, fruit
Glue, tannin and alkaloid etc..There are 11 kinds of amino acid, 11 kinds of mineral elements, wherein needed by human is micro in the starch of pueraria lobata
Member is known as Fe, Cu, Zn, Mn, Si etc..Contain isoflavones components in Pueraria Flavonid, it is the main active of pueraria lobata.
Kudzu root extract is generally used for anti-aging cosmetics, can make facial smoothness, have the effect of except acne eliminating wrinkle,
Its mechanism is mainly with pueraria isoflavone class with anti-oxidant, enhancing immunity of organisms is related.Pueraria isoflavone, which has, removes oxygen certainly
The effect of by base and anti peroxidation of lipid, can significantly inhibit erythrocyte hemolysis caused by oxidative damage, inhibit the work to microsome
Property oxygen class (- OH, O2-) caused by peroxidization generation, and can be improved internal SOD activity intensity, adjust internal oxygen from
By the balance of base and free radical scavenger, thus pueraria lobata has preferable anti-aging effects.
Grass is brought back to life generally in the case where extreme drought, its branches and leaves can tightly shrink glomeration, firmly lock precious in vivo
Last expensive drips, though lose itself be up to 98% moisture, still can indomitable existence many decades.Once encountering rare rain
It season brings back to life in cursive script and contains glycosyl trehalose abundant and will play powerful water lock moisture-keeping functions, then will contain life again
The water of vigor is smoothly living freely to be imported in each cell in vivo, and latching is equivalent to 460 times of cell own wt of moisture,
Branches and leaves can be re-expanded, full of life green life is restored.
It brings back to life careless extract to apply in cosmetics, the immune function of skin is adjusted, enhances the disease resistance of skin, disappears
Except inflammation, pre- hypo-allergenic, cleaning skin, while cosmetics and other extraneous factors can also be released to the toxic side effect of skin.
In addition, cell viability and metabolism, activating and regenerating Skin Cell, enhancing skin can also be enhanced by bringing back to life careless extract
The inoxidizability of skin cell mitigates rubefaction, and confrontation erythema plays the role of accelerating wound healing and tissue repair, can also subtract
Collagen production quantity in aged cells can be improved 36% by the ager process of slow skin corium fibrocyte.
The present invention has synergistic effect in the combination using kudzu root extract and the careless extract of resurrection, the two, can be into one
Step plays the role of inhibiting tyrosinase activity.In the present invention, in terms of the gross mass of the tyrosinase inhibitor, with described
The gross mass meter of tyrosinase inhibitor, the additional amount of the kudzu root extract are 0.5-75%, the careless extract of resurrection
Additional amount is 25-99.5%.In the present invention, kudzu root extract and the careless extract of resurrection can be combined with arbitrary proportion.
Preferably, the kudzu root extract and the mass ratio for bringing back to life careless extract are 1:0.4~90, preferably 1:
1~80, more preferably 1:2~70, further preferably 1:2.5~60 are still more preferably 1:3~55, further excellent
It is selected as 1:3.5~50.When kudzu root extract is with the mass ratio of careless extract is brought back to life within the above range, can further have
Synergistic effect, the excellent effect inhibited tyrosinase activity.
Second embodiment
The whitening that second embodiment of the present invention provides a kind of tyrosinase inhibitor including first embodiment is bright
Skin water.The present invention is able to suppress the activity of tyrosinase, is made by the tyrosinase inhibitor of the invention of addition appropriate level
Obtaining whitening skin lightening water of the invention has excellent whitening effect.
Wherein, in terms of the gross mass of the whitening skin lightening water, the additional amount of the tyrosinase inhibitor is 0.01-
12%, preferably 0.1-10%, such as: 0.5%, 1%, 2%, 3%, 5%, 6%, 7%, 8% etc..Work as tyrosinase inhibitor
Additional amount between 0.01-12% when, reduced using melanin content in the skin after whitening skin lightening water.When tyrosinase presses down
When the additional amount of preparation is less than 0.01%, the content reduction amount of melanin is few, cannot play the role of whitening;When tyrosinase presses down
When the additional amount of preparation is greater than 12%, the too high levels of tyrosinase inhibitor, cost is excessively high, and corresponding whitening effect mentions
It rises unobvious.
Whitening skin lightening water of the invention can also include moisturizer, thickener, solubilizer, skin conditioning agent, Shu Min agent with
And preservative.The effect of formula composition of whitening skin lightening water of the invention is mild, can make tyrosinase inhibitor of the invention
It is not fully exerted.Specifically:
In terms of the gross mass of the whitening skin lightening water, the additional amount of the moisturizer is 0.01-20%.When adding for moisturizer
Enter amount in 0.01-20%, moisturizing moisturizing can be played the role of.The effect of in order to enable moisturizer, further plays, and makees
To be preferred, the additional amount of moisturizer of the invention can be 1-19%, can be 4-18%, can be 6-17%, can be 8-
16%, it can be 10-15% etc..When the content of moisturizer is lower than 0.01%, moisture-keeping function is unobvious;When the content of moisturizer
When higher than 20%, whitening skin lightening water can be made to have glutinous soapy feeling.
In the present invention, the moisturizer includes dipropylene glycol, dipropylene glycol, propylene glycol, butanediol, panthenol, glycerol, gathers
The combination of one or more of ethylene glycol -32, glycerin polyether -26, Sodium Hyaluronate.Use the group of a variety of moisturizer
It closes, can make whitening skin lightening water of the invention that there is excellent performance of keeping humidity.
In terms of the gross mass of the whitening skin lightening water, the additional amount of the thickener is 0.02-0.8%.When thickener
When additional amount is between 0.02-0.8%, there is low sticky sense and excellent use feeling, good dispersion absorbs fast.Work as thickener
Additional amount less than 0.02% when, the quality of whitening skin lightening water is diluter, without any sticky sense;When the additional amount of thickener is greater than
When 0.8%, whitening skin lightening water is excessively thick and heavy, will increase the burden of skin.
In the present invention, the thickener includes acrylic acid (ester) class/C10-30 alkylol acrylamide acid esters cross-linked polymer, hydroxyl
Ethyl cellulose, xanthan gum, hydroxy-ethyl acrylate/sodium acryloyldimethyl taurate copolymers, carbomer and acryloyl diformazan
One or more of base taurine ammonium/VP copolymer.
In terms of the gross mass of the whitening skin lightening water, the additional amount of the solubilizer is 0.01-0.5%.In whitening skin lightening
Solubilizer is used in water, its skin sense can be made more smooth.Preferably, the additional amount of the solubilizer is 0.05-0.4%,
0.08-0.3% etc..In the present invention, the solubilizer includes polysorbate -20, Cremophor RH40 and PPG-26-
One or more of butylol polyether -26.
The effect of whitening skin lightening water in order to further increase the application, whitening skin lightening water of the invention also includes skin
Conditioner.By adding skin conditioning agent, the effect of moisturizing moisturizing can be further functioned as, and the generation of wrinkle can be reduced.
In addition, skin conditioning agent can also suitably reduce the adhesion strength of corneocyte, accelerates epidermal cell to update, enhance repairing for skin
Reactivation power.
In terms of the gross mass of the whitening skin lightening water, the additional amount of the skin conditioning agent is 0.01-5%, such as can be with
It is 0.5%, 1%, 2%, 3%, 4% etc..When the additional amount of skin conditioning agent is less than 0.01%, content is too low, can not play
Corresponding effect.
In the present invention, the skin conditioning agent includes bulk kelp extract, vitamin E, oat peptide, Cer NS, Mo Jiao
Algae extract, chlorella tunning, Hydrolyzed Collagen, hydrolyzed wheat protein, green algal extract, brown alga extract, allantois
Element, Bacillus acidi lactici/fermented soybean product extract, Herba Visci leaf extract, cogongrass rhizome extractive, Radix et Caulis Opuntiae Dillenii extract,
The combination of one or more of trehalose.
Wherein, bulk kelp extract is derived from bulk kelp, and bulk kelp improves the day of its high-content by the mass propagation of macroalgal cells
Right vegetable protein.Bulk kelp extract has effects that supplement cytotrophy, the effect of effective moisturizing.
Wherein, active smegma can be inhibited while improving sebum film using Cer NS, keeps skin water oily
Balance enhances the self-protection function of skin, is relatively suitble to skin greasing and has the young skin of demand.The ingredient is to skin moisturizing
Maintenance has good action, is skin-active ingredient important in cuticula, can enhance skin barrier, reconstituted cell.
Wherein, allantoin can reduce the adhesion strength of corneocyte, accelerate epidermal cell to update, enhance the reparation of skin
Ability, and its degree of safety is high.
Wherein, oat peptide is the segment of avenin and the component part of avenin.The function of avenin is unique, it
Amino acid classes equilibrium far win other cereal.Avenin can generate the higher small-molecular peptides of quality after decomposing, easily by skin
Skin absorbs.Oat peptide has anti-oxidant, increase skin activity, delaying skin aging, moisturizing, reduction wrinkle, antiallergy and other effects.
Wherein, the albumen of amino acid wheat alcohol soluble protein (gliadin) and Mai Gu are contained in hydrolyzed wheat protein, and abundant
Cystine (cystine).Hydrolyzed wheat protein has the multiple efficacies such as moisturizing, anti-oxidant and soft refinement skin, but also
Containing special moisturizing ingredient, wrinkle can be improved.
In addition, whitening skin lightening water of the invention can also add Shu Min agent.By adding Shu Min agent, can calm skin, from
And there is certain Shu Min to act on facial skin injury redness, it can help that skin is anti-inflammatory, releives and promotes cell repair.
In terms of the gross mass of the whitening skin lightening water, the additional amount of the Shu Min agent is 0.01-5%, such as be can be
0.5%, 1%, 2%, 3% etc..When the additional amount of Shu Min agent is less than 0.01%, Shu Min effect is unobvious.When adding for Shu Min agent
When entering amount greater than 5%, the effect of Shu Min cannot be further functioned as, and cost is excessively high.
The Shu Min agent includes hamamelis water, camomile extract, purslane extract, Gotu Kola P.E, ginger
Root extract, bisabolol, the combination of one or more of dipotassium glycyrrhizinate.
Wherein, the hamamelis flower water in Shu Min agent belongs to high power concentration plant former dew, has light herbal, tool
There is the effect of controlling and balancing oil, convergence is compact, pore refining and eliminating stasis to resolve swelling.Shu Min agent flesh is pacified in the nursing that can be used in skin
Skin.
Whitening skin lightening water of the invention can also be properly added pH adjusting agent, antioxidant and chelating agent.In general,
In terms of the gross mass of the whitening skin lightening water, the additional amount of the pH adjusting agent is 0-1%;The additional amount of the antioxidant is
0-2%;The additional amount of the chelating agent is 0-1%.The pH adjusting agent includes aminomethyl propanol, arginine, citric acid, lemon
The combination of one or more of sour sodium, sodium hydroxide etc..The antioxidant can be vitamin E, tocopheryl acetate
The combination of one or more of ester, Butylated Hydroxytoluene, lycopene etc..The chelating agent can be EDTA-2Na and/or
EDTA-4Na etc..
The additional amount of preservative in whitening skin lightening water of the invention is 0.01-1.5%.The preservative may include benzene
The combination of one or more of oxyethanol, methyl hydroxybenzoate, Nipasol, benzoic acid and its salt.In addition, beauty of the invention
Also contain aromatic in white skin lightening water.In terms of the gross mass of the whitening skin lightening water, the additional amount of the aromatic is 0.005-
0.5%.The aromatic can be essence etc..
Third embodiment
Second embodiment of the present invention also provides a kind of preparation method of whitening skin lightening water according to the present invention, including will
The step of each component mixing of the whitening skin lightening water.
Specifically, the preparation method of whitening skin lightening water of the invention may comprise steps of:
1, Jiang Shui, moisturizer, thickener, partial skin conditioner, the chelating agent being optionally present, the pH being optionally present are adjusted
Agitated kettle is added in agent, aromatic and part preservative raw material, is stirred and heated to 80-85 DEG C;
2, it is cooled to 40-50 DEG C, tyrosinase inhibitor is added, solubilizer, remaining skin conditioning agent, Shu Min agent, appoints
Existing antioxidant and remaining preservative is selected to stir evenly;
3, it is cooled to 35-40 DEG C, is discharged after the assay was approved, stands 12-48 hours;
4, it after the assay was approved, dispenses, pack, then examine, warehousing finished products.
Embodiment
Embodiment of the present invention is described in detail below in conjunction with embodiment, but those skilled in the art will
Understand, the following example is merely to illustrate the present invention, and should not be taken as limiting the scope of the invention.It is not specified in embodiment specific
Condition person carries out according to conventional conditions or manufacturer's recommended conditions.Reagents or instruments used without specified manufacturer is
It can be with conventional products that are commercially available.
Comparative example 1-5
Take kudzu root extract as tyrosinase inhibitor.It is 6.8 by the pH that kudzu root extract is dissolved in 5 groups of different volumes
In phosphate buffer, the test solution of 5 groups of various concentrations is obtained.Wherein, the logarithm mass concentration of kudzu root extract such as the following table 2 institute
Show.
Comparative example 6-9
Take the careless extract of resurrection as tyrosinase inhibitor.To bring back to life careless extract be dissolved in 4 groups it is corresponding with comparative example 1-4
In the phosphate buffer that the pH of volume is 6.8, the test solution of 4 groups with comparative example 1-4 corresponding concentration is obtained.Wherein, grass is brought back to life
The logarithm mass concentration of extract is as shown in table 2 below.
Embodiment 1-7
It takes kudzu root extract and brings back to life careless extract as tyrosinase inhibitor.By kudzu root extract and bring back to life grass extraction
Object 2:1 in mass ratio (embodiment 1), 1:1 (embodiment 2), 1:2 (embodiment 3), 15:85 (embodiment 4), 5:95 (embodiment
5), after 2:98 (embodiment 6), 7:68 (embodiment 7) mixing, tyrosinase inhibitor is obtained.By the tyrosinase of embodiment 1-7
Inhibitor is dissolved in the pH of 5 groups and comparative example 1-5 corresponding volume respectively in 6.8 phosphate buffer, embodiment 1-7 can be obtained
To 5 groups and the test solution of comparative example 1-5 corresponding concentration.Wherein, in tyrosinase inhibitor, kudzu root extract and resurrection grass are mentioned
Taking the content (quality %) of object, the logarithm mass concentration of tyrosinase inhibitor is as shown in table 3 below as shown in table 1 below.
Table 1
Testing in vitro
Tyrosinase activity is measured with being catalyzed the tyrosinase vigor of L-3,4 dihydroxyphenylalanine generation DOPA quinone.Specifically:
1 test tube is taken, is added substrate L-3,4 dihydroxyphenylalanine solution 0.4mL (1.0mg/mL), and adds the phosphate buffer that pH is 6.8
After 2.4mL, 30 DEG C of water-bath heat preservation 10min, the amount that tyrosinase is then added is 0.2mL (250U/mL), obtains reaction solution 1.
1 test tube is taken, the amount that tyrosinase is added is 0.2mL (250U/mL), and adds the phosphate buffer that pH is 6.8
2.8mL obtains reaction solution 2.
Substrate L-3,4 dihydroxyphenylalanine solution 0.4mL (1.0mg/mL) is added in 44 test tubes respectively, is then respectively adding 2.4mL's
The test solution of comparative example 1-9 and embodiment 1-7, after 30 DEG C of water-baths keep the temperature 10min, the amount that tyrosinase is then added is
0.2mL (250U/mL), obtains reaction solution 3.
The test solution of the comparative example 1-9 and embodiment 1-7 of 2.4mL is added in 44 test tubes respectively, and adds pH and is
After 6.8 phosphate buffer 0.4mL, 30 DEG C of water-bath heat preservation 10min, the amount that tyrosinase is then added is 0.2mL (250U/
ML), reaction solution 4 is obtained.
L-3,4 dihydroxyphenylalanine is converted red product DOPA quinone by enzymatic reaction, there is absorption maximum at 475nm.Reading is opened from mixing
Absorbance A when beginning 1min at 475nm475, the speed of enzymatic reaction A per minute475Value added indicate.It measures above-mentioned
Absorbance A of the reaction solution when reaction starts 1min at 475nm, and the inhibition of tyrosinase activity is calculated as follows
Rate.Wherein, the mass concentration of the tyrosinase inhibitor in table is whole quality of the tyrosinase inhibitor in test system
Concentration.
Inhibiting rate=[1- (A3-A4)/(A1-A2)] × 100%
In formula: A1Not add tyrosinase inhibitor, only containing substrate when absorbance;
A2Absorbance not add tyrosinase inhibitor, and when not adding substrate;
A3To contain tyrosinase inhibitor and absorbance when substrate;
A4Absorbance to contain tyrosinase inhibitor, and when not adding substrate.
It calculates separately to obtain kudzu root extract (comparative example 1-5) and the careless extract (comparative example 6-9) of resurrection is respective to junket
The inhibiting rate of propylhomoserin enzymatic activity.And logarithm mass concentration-tyrosinase inhibition rate relational graph is combined, conversion acquires pueraria lobata extraction
The inhibiting rate of object corresponding mass concentration (IC when being 50%50A) and bring back to life corresponding matter when the inhibiting rate of careless extract is 50%
Measure concentration (IC50B), the results are shown in Table 2.
Table 2
Then the inhibiting rate to tyrosinase activity of the tyrosinase inhibitor of embodiment 1-7 is measured.And combine logarithm
Mass concentration-tyrosinase inhibition rate relational graph, conversion acquire kudzu root extract and bring back to life careless extract compound action and generate
When equivalent inhibiting rate (50%), the mass concentration (IC of kudzu root extract50a), kudzu root extract and the compound work of the careless extract of resurrection
When with generating equivalent inhibiting rate (50%), the mass concentration (IC of careless extract is brought back to life50b), the results are shown in Table 3.
The effect of the compound action of kudzu root extract and the careless extract of resurrection, can be evaluated with interaction coefficient γ,
Concrete outcome is as shown in table 3.
γ=IC50a/IC50A+IC50b/IC50B
Wherein, IC50AThe inhibiting rate for indicating kudzu root extract corresponding mass concentration when being 50%;
IC50BIndicate to bring back to life corresponding mass concentration when the inhibiting rate of careless extract is 50%;
IC50aWhen indicating kudzu root extract and bringing back to life careless equivalent inhibiting rate (50%) of extract compound action generation, pueraria lobata is mentioned
Take the mass concentration of object;
IC50bWhen indicating kudzu root extract and bringing back to life careless equivalent inhibiting rate (50%) of extract compound action generation, grass is brought back to life
The mass concentration of extract.
Wherein, γ=1 indicates that kudzu root extract and the careless extract of resurrection show as simple additive effect;γ < 1 is pueraria lobata
Extract and the careless extract of resurrection show as synergistic effect, and γ value is smaller, and synergistic effect is stronger;γ > 1 be kudzu root extract and
It brings back to life careless extract and shows as antagonistic effect, γ value is bigger, and antagonistic effect is bigger.
Table 3
As can be seen from Table 3, the interaction coefficient in tyrosinase inhibitor of the invention is less than 1, and its phase interaction
It can be 0.65 hereinafter, and can be 0.5 hereinafter, therefore kudzu root extract and the careless extract of resurrection can show with coefficient value
For excellent synergistic effect.
Application Example 1-8
According to content (the quality percentage of each component in the whitening skin lightening water of the Application Example 1-8 in the following table 4-5 formula
Than), and whitening skin lightening water is prepared according to following production craft steps.Production craft step is as follows:
1, agitated kettle is added in A phase, B phase and C phase raw material, is stirred and heated to 82-85 DEG C;
2,42 DEG C are cooled to, D phase, E phase, F phase is added and stirs evenly;
3,37 DEG C are cooled to, is discharged after the assay was approved, stands 24 hours;
4, it after the assay was approved, dispenses, pack, then examine, warehousing finished products.
Note: A phase, B phase, C phase, D phase, E phase, F phase in technique be respectively,
A phase: water;
B phase: butanediol, glycerol, propylene glycol, hydroxy-ethyl acrylate/sodium acryloyldimethyl taurate copolymers, acryloyl
Dimethyltaurine ammonium/VP copolymer, Sodium Hyaluronate, panthenol, dipropylene glycol;
C phase: methyl hydroxybenzoate, allantoin, glycerin polyether -26, polyethylene glycol -32;
D phase: Cremophor RH40, essence;
E phase: Phenoxyethanol, Cer NS, trehalose;
F phase: kudzu root extract brings back to life careless extract, bulk kelp extract, hamamelis flower water, hydrolyzed wheat protein, swallow
Wheat peptide.
Kudzu root extract used in formula, the careless extract of resurrection are tyrosinase inhibitors;
Butanediol, glycerol, propylene glycol, Sodium Hyaluronate, panthenol, dipropylene glycol, glycerin polyether -26, polyethylene glycol -32 are
Moisturizer;
Hydroxy-ethyl acrylate/sodium acryloyldimethyl taurate copolymers, ammonium acryloyl taurate/VP copolymer
It is thickener;
Bulk kelp extract, Cer NS, hydrolyzed wheat protein, oat peptide, allantoin, trehalose are skin conditioning agents;
Cremophor RH40 is solubilizer;Hamamelis flower water is Shu Min agent;
Phenoxyethanol, methyl hydroxybenzoate are preservatives;Essence is aromatic.
In the present invention, kudzu root extract, manufacturer: Zhan Hong Biotechnology Co., Ltd of Quzhou City;
Bring back to life careless extract, manufacturer: Zhan Hong Biotechnology Co., Ltd of Quzhou City.
Table 4
Comparison study example 1-2
According to the content (mass percent) of each component in the skin lightening water of the Comparison study example 1-2 in the following table 5 formula, and press
According to method identical with Application Example 1-8, skin lightening water is prepared.
Table 5
White-skinned face function test
The test method of dermal melanin change rate: by measure specific wavelength illumination on human skin after reflection
Measure the content to determine melanin in skin.It is respectively tri- kinds of 568nm, 660nm and 880nm that the transmitter of probe, which issues wavelength,
The light of wavelength is radiated at skin surface, and recipient measures the light of skin reflex, and measures the amount for the light being absorbed by the skin, so as to
To obtain the change rate of dermal melanin.
The test of dermal melanin change rate is tested using the detector for pigment of CK company of Germany, wherein skin is black
The test probe MX 18 of pigment is made of light source emitter and receiver, to the pressure of skin when having spring separately to keep detection
It is constant.The measurement range of probe is 0-999, and measurement numerical value is higher, it may be said that the content of melanin is higher in bright skin.
Number of subjects be 33 people, the test period be 4 weeks, test selection Application Example 1-8 whitening skin lightening water and
The skin lightening water of Comparison study example 1-2, is applied to the different zones of inner forearm sooner or later daily, respectively before measurement experiment (Day1) and
Using volume reflection of the skin in the tested region of (Day28) at 28 days after the illumination of specific wavelength to determine melanin in skin
Content, and then characterize the change rate of melanin, the result of the change rate of specific melanin is as shown in Figure 5.
As seen from Figure 5, the change rate of the melanin of the Application Example 1-8 of the application is larger, i.e. melanin content
It reduces, therefore, uses kudzu root extract and the careless extract of resurrection as tyrosinase inhibitor, it being capable of whitening effect skin.
In addition, in the Application Example 6-8 of the application, since the content of kudzu root extract is higher, melanin content drop
It is low less.
In the Comparison study example 1-2 of the application, careless extract is brought back to life in single use kudzu root extract or single use
When as tyrosinase inhibitor, melanin content change rate is small, and melanin content reduces less, and whitening effect is poor.
The above embodiment of the present invention be only to clearly illustrate example of the present invention, and not be to the present invention
Embodiment restriction.For those of ordinary skill in the art, it can also make on the basis of the above description
Other various forms of variations or variation.There is no necessity and possibility to exhaust all the enbodiments.It is all of the invention
Made any modifications, equivalent replacements, and improvements etc., should be included in the protection of the claims in the present invention within spirit and principle
Within the scope of.