CN107837206A - Fermentation composition containing human stem cell growth factor, preparation method and cosmetics - Google Patents
Fermentation composition containing human stem cell growth factor, preparation method and cosmetics Download PDFInfo
- Publication number
- CN107837206A CN107837206A CN201711278680.3A CN201711278680A CN107837206A CN 107837206 A CN107837206 A CN 107837206A CN 201711278680 A CN201711278680 A CN 201711278680A CN 107837206 A CN107837206 A CN 107837206A
- Authority
- CN
- China
- Prior art keywords
- fermentate
- parts
- rice bran
- fermentation composition
- peptide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/52—Stabilizers
- A61K2800/522—Antioxidants; Radical scavengers
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Dermatology (AREA)
- Epidemiology (AREA)
- Birds (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Cosmetics (AREA)
Abstract
The invention relates to the technical field of skin care products, in particular to a fermented composition containing a human stem cell growth factor, a preparation method thereof and a cosmetic. The fermentation composition is prepared from the following components in parts by weight, wherein the fermentation composition is prepared from the following components in parts by weight: 1-10 parts of soybean peptide leavening, 1-10 parts of oat peptide leavening, 10-20 parts of rice bran extract, 10-20 parts of rice bran leavening, 5-15 parts of ginseng fruit shell leavening, 5-15 parts of ginseng root leavening, 5-15 parts of ginseng stem leaf leavening, 1-10 parts of ginkgo biloba leavening, 1-10 parts of tremella leavening, 0.5-5 parts of grape seed leavening, 0.01-0.1 part of human stem cell growth factor SCGF and the balance of water. Also provided is a cosmetic, the raw material of which comprises the fermented composition of the invention.
Description
Technical field
The present invention relates to skin care product technical field, and in particular to a kind of fementative composition containing human stem cell growth
Thing, its preparation method and cosmetics.
Background technology
People particularly women extremely focuses on keeping skin vigorous and graceful.But skin is but always subjected to many factors, example
Such as the influence of humidity, ultraviolet, cosmetic composition, age, disease, anxiety and eating habit.As a result, skin occurs
Many troubles, such as the multiple functions decline of skin and skin senescence.
Skin senescence is by endogenous factor and the coefficient result of extrinsic factor.Wherein during organism metabolism
The caused free radical with elevated chemical activity is the important startup factor of aging.Free radical is one kind because losing an electronics
Asymmetric, the unstable atom or molecule formed, there is very big energy, lipid that can be in attack cells tissue, protein,
The material such as carbohydrate and DNA, attempt capture an electronics in the hope of rebalancing, and this has resulted in the oxidation of lipid and carbohydrate, egg
The denaturation of white matter, the inactivation of enzyme, the cut-out of DNA structure, base change etc., so as to cause the damage of cell membrane, gene etc.
Wound, and then induce various diseases, canceration, organism aging process etc..Irradiated for a long time by ultraviolet, environmental pollution and smoke from cigarette etc. it is outer
Source sexual factor also can all produce a large amount of free radicals, cause skin senescence.
So far, it has been suggested that many compositions and method are used to prevent or treat aging effect, however, current market
On product still can not meet the needs of people comprehensively.
The content of the invention
In view of this, it is necessary to for it is above-mentioned the problem of, there is provided a kind of fermentation composition containing human stem cell growth,
Its preparation method and cosmetics.
The present invention provides a kind of fermentation composition containing human stem cell growth, with the mass parts of the fermentation composition
Number is 100, and the fermentation composition is made up of the component of following parts by weight：1-10 part Soybean Peptide fermentates, 1-10 part oat peptides
Fermentate, 10-20 part rice bran extracts, 10-20 part rice bran fermentates, 5-15 part ginseng shell fermentates, 5-15 part ginsengs
Fermentate, 5-15 part gen-seng haulms fermentates, 1-10 part ginkgo fermentates, 1-10 part Tremella fermented products, 0.5-5 parts grape pip hair
Ferment thing is with the water of 0.01-0.1 part human stem cell growth SCGF and surplus.
Preferably, the Soybean Peptide fermentate and the oat peptide fermentate by fermentation of bacillus subtilis Soybean Peptide and
Oat peptide obtains.
Preferably, the oat peptide using enzymic degradation from oat bran by extracting to obtain.
Preferably, the Soybean Peptide through everfermentation or enzyme engineering by handling to obtain pure natural soybean.
Preferably, the Tremella fermented product includes tremella polysaccharides, described using the quality of the Tremella fermented product as 100%
The weight/mass percentage composition of tremella polysaccharides is not less than 80%.
Preferably, the rice bran extract includes inositol, vitamin B, phytic acid and mineral matter.
Preferably, the mineral matter includes potassium, magnesium, sodium, calcium, iron, selenium and zinc；The vitamin B includes vitamin B2.
Preferably, the rice bran fermentate includes the protein and forulic acid of high activity.
The present invention also provides a kind of preparation method of above-mentioned fermentation composition, and the preparation method includes：Prepare respectively big
Beans peptide fermentate, oat peptide fermentate, rice bran extract, rice bran fermentate, ginseng shell fermentate, ginseng fermentate, people
Join cauline leaf fermentate, ginkgo fermentate, Tremella fermented product, grape seed fermented material and human stem cell growth SCGF；Will be big
Beans peptide fermentate is well mixed with oat peptide fermentate, and A phases are made；Rice bran extract is well mixed with rice bran fermentate and is made
B phases；Ginseng shell fermentate, ginseng fermentate are well mixed with gen-seng haulms fermentate, C phases are made；By A phases, B phases, C
Mix uniformly, add the human stem cell growth SCGF, water bath with thermostatic control, stir；Add ginkgo fermentate, silver
Ear fermentate and grape seed fermented material, heated at 60 DEG C -80 DEG C well mixed.
The present invention also provides a kind of cosmetics in addition, and the raw material of the cosmetics includes above-mentioned fermentation composition.
The beneficial effects of the present invention are：Using the fermentation composition containing human stem cell growth of the present invention as former
Material prepares cosmetics, it is ensured that obtained cosmetics are entirely free of preservative, artificial color, essence and flavoring agent, mineral oil etc. pair
Skin has a dysgenic chemical components, and the raw material in fermentation composition is substantially all to be extracted from nature, safe and non-stimulating.
Obtained cosmetics not only have good antioxygenic property, can speed up the new life of epidermis, promote the discharge of melanin, play
The effect of crease-resistant reparation, make skin regain compact it is fine and smooth, take leave of it is dim mute coarse, allow skin recover youth as health status, and
And good whitening and moistening effect can also be played, and preparation method is simple, has very good application prospect.
Embodiment
For the ease of it will be appreciated by those skilled in the art that further being retouched to the present invention below in conjunction with specific embodiment
State.
The present invention provides a kind of fermentation composition containing human stem cell growth, with the mass parts of the fermentation composition
Number is 100, and the fermentation composition is made up of the component of following parts by weight：1-10 part Soybean Peptide fermentates, 1-10 part oat peptides
Fermentate, 10-20 part rice bran extracts, 10-20 part rice bran fermentates, 5-15 part ginseng shell fermentates, 5-15 part ginsengs
Fermentate, 5-15 part gen-seng haulms fermentates, 1-10 part ginkgo fermentates, 1-10 part Tremella fermented products, 0.5-5 parts grape pip hair
The water of ferment thing, 0.01-0.1 part human stem cell growth SCGF and surplus.
In further preferred embodiment, human stem cell growth SCGF mass fraction is 0.01-0.05 parts.
Human stem cell growth can be the restructuring human stem cell growth prepared with gene engineering method, can also
The naturally isolated people source human stem cell growth of application cell nutrient solution, human stem cell growth include hSCGF- α and
Two hypotypes of hSCGF- β.Specifically, human stem cell growth SCGF is prepared via a method which to obtain：Filled between people's umbilical cord
Extracted total RNA in matter stem cell, reverse transcription cDNA；According to people's SCGF full-length gene orders, i.e. GenBank Accession
No.NM_002975, design PCR primer；SCGF1 upstream primer sequences：5 '-CAAGAGTCCAGCTTAATGCAGGCAG-3 ',
SCGF1 downstream primer sequences：5′-GCTAGAAGGGGAACTCGCAGACGTA-3′；It is template that the above, which obtains CDNA, passes through PCR
Obtain SCGF1 genes；SCGF mature polypeptide coding sequences PCR primer designs：SCGF2 upstreams：5′-
ATTTAAAACATATGGGTGCTCGGGGAGCAGAGAG-3 ', SCGF2 downstream：5′-
ATTGGATCCCTAGAAGGGGAACTCGCAG-3′；Using SCGF1 as template, PCR reactions obtain SCGF2；With Nde I, BamH I couple
Digestion SCGF2 and pET-28a (+) plasmid, T4DNA ligases connection, attachment transformed competence colibacillus bacillus coli DH 5 alpha, recombinates matter
After grain pET-28a-SCGF is successfully constructed, e. coli bl21 (DE3) expression is transformed into；The recombined human stem cell growth of expression because
Son passes through Ni2+- NTA posts purify, and obtain target recombinant protein.
Oat, grass family swallow race Avena, annual or herbaceos perennial, cereals nutrient value is abundant and comprehensive,
The Mineral Concentrations such as its protein, fat, vitamin E, dietary fiber and calcium, magnesium, iron, phosphorus are above other cereals and made
Thing, there is reducing blood lipid, reduce a variety of raw functions such as cholesterol, hypotensive, hypoglycemic and inoxidizability；In embodiment,
The molecular weight of oat peptide employed in fermentation composition is 1000~3000Da, and oat peptide is specifically to be used from oat bran
Enzymic degradation extracts to obtain, and the specific steps of enzymic degradation extraction include：1) pre-process：By oat bran plus water, regulation pH is arrived
8-10, in 45-55 DEG C of heating；2) ferment treatment：Alkali protease is added in by pretreated liquid, in 45-55
Stir process 1-5 hours at DEG C；Then, papain, the stir process 1-5 hours at 45-55 DEG C are added；3) filtering and film
Processing：It will be filtered by the liquid of ferment treatment, then, the milipore filter ultrafiltration using molecular cut off as 30000-50000Da, then
NF membrane nanofiltration using molecular cut off as 600-800Da removes institute's saliferous and concentrated, and obtains oat peptide.Soybean Peptide be pass through by
Pure natural soybean handles to obtain through everfermentation or enzyme engineering, and pure natural Soybean Peptide then has activation epidermal basal cell division work(
Effect.
In embodiment, Soybean Peptide fermentate and the oat peptide fermentate pass through fermentation of bacillus subtilis soybean
Peptide and oat peptide obtain.
Rice bran is the principal by product of paddy processing, is processed into by kind of skin and embryo.In rice bran rich in various nutrients and
Physiological activator；Averagely contain protein 15% in rice bran, fatty 16%-22%, sugared 3%-8%, moisture 10%, heat is about
For 125.1KJ/g, rice bran has certain white-skinned face function by fermenting containing abundant amino acid and peptide material.
Specifically, the rice bran extract employed in the fermentation composition of the present invention is the powdered thing extracted from rice bran
Matter, wherein containing protein, inositol, vitamin B, phytic acid and mineral matter, mineral matter includes potassium, magnesium, sodium, calcium, iron, selenium, zinc etc.,
It with antioxidation, and can effectively suppress tyrosinase activity, prevent melanin from producing, its vitamin B2 and inositol for containing
The new life of epidermis can be accelerated, promote the discharge of melanin.Rice bran fermentate is the distiller's yeast by being saccharified in brewed rice wine, and with make
Brewer yeast and Lactobacillus rhamnosus help what fermentation was produced, and it contains the protein of high activity and forulic acid, can significantly reduce MITF
The activity of (microphthalmia collaboration transcription factor), and MITF is main tyrosinase transcription factor, so as to suppress junket ammonia
Phytase activity, block melanin to be formed, prevent pigementation.
White fungus is that China's economic value is high, and a kind of precious edible mushroom and medicinal fungus, is traditional edible and medicinal fungi.
And the tremella polysaccharides mainly contained in Tremella fermented product is then a kind of important bioactive substance.Tremella polysaccharides can substantially drop
Free cholesterol, the content of cholesteryl ester in low high fat of blood, serum, hence it is evident that strengthen the immunologic function of body, promote liver cell sugar
Original synthesis and antitumor, anti-mutation, radioresistance and increasing leukocyte.Moreover, tremella polysaccharides has certain removing hydroxyl free
The ability of base, it can be applied to as aging ingredient in cosmetics, in main active α-mannosan in tremella polysaccharides
Again containing substantial amounts of hydroxyl, carboxyl isopolarity group, substantial amounts of moisture can be combined so that the moistening effect of tremella polysaccharides is also obvious
Better than glycerine and trehalose.In the fermentation composition of the present invention, the weight/mass percentage composition of tremella polysaccharides is not low in Tremella fermented product
In 80%, anti-aging and moisturizing can be played a part of preferably with other fermentate combinations.
Ginseng contains substantial amounts of polysaccharide and ginsenoside composition, and in the different parts in ginseng plant, ginsenoside contains
There were significant differences for amount, present inventors have unexpectedly found that, by ginseng shell fermentate, ginseng fermentate and gen-seng haulms fermentate three
Person is used in combination, and can produce relative to the simple more preferable effect of ginseng fermentate.Specifically, the side of solid fermentation flower of Panax ginseng
Method, using Lactobacillus plantarum in solid medium fermented ginseng shell, ginseng or gen-seng haulms, come prepare ginseng shell hair
Ferment thing, ginseng fermentate and gen-seng haulms fermentate, it can preferably improve content of ginsenoside in fermentate.
Given birth to by cereals fermentate, ginseng class fermentate, plant fermentate, fruits fermentate and human stem cell
Long factor S CGF is used in mixed way according to specific ratio, and synergy, obtained fermentation composition not only has well anti-ageing
Old effect, moreover it is possible to while there is good white-skinned face function and moisture-keeping efficacy.
The present invention also provides the present invention and also provides a kind of preparation method of above-mentioned fermentation composition, specifically includes：Make respectively
Standby Soybean Peptide fermentate, oat peptide fermentate, rice bran extract, rice bran fermentate, ginseng shell fermentate, ginseng fermentation
Thing, gen-seng haulms fermentate, ginkgo fermentate, Tremella fermented product, grape seed fermented material and human stem cell growth SCGF；
Soybean Peptide fermentate is well mixed with oat peptide fermentate, A phases are made；Rice bran extract is well mixed with rice bran fermentate
B phases are made；Ginseng shell fermentate, ginseng fermentate are well mixed with gen-seng haulms fermentate, C phases are made；By A phases, B
Phase, C are mixed uniformly, are added the human stem cell growth SCGF, 30 DEG C of -40 DEG C of water-baths of constant temperature, are stirred；Add
Ginkgo fermentate, Tremella fermented product and grape seed fermented material, heated at 60 DEG C -80 DEG C well mixed.Fermentation group of the present invention
The preparation method step of compound is simple, strong applicability, has a good application prospect.
Cosmetics are prepared as raw material using the fermentation composition containing human stem cell growth of the present invention, it is ensured that
Obtained cosmetics be entirely free of preservative, artificial color, essence and flavoring agent, mineral oil etc. have to skin dysgenic chemical industry into
Divide, the raw material in fermentation composition is substantially all to be extracted from nature, safe and non-stimulating.Obtained cosmetics not only have good
Good antioxygenic property, can speed up the new life of epidermis, promotes the discharge of melanin, play the effect of crease-resistant reparation, make skin
Regain compact it is fine and smooth, take leave of it is dim mute coarse, allow skin recover youth as health status, and can also play good whitening and
Moistening effect, and preparation method is simple, has very good application prospect.
It is further described below in conjunction with specific embodiment.
Embodiment 1
Soybean Peptide fermentate, oat peptide fermentate, rice bran extract, rice bran fermentate, the fermentation of ginseng shell are prepared respectively
Thing, ginseng fermentate, gen-seng haulms fermentate, ginkgo fermentate, Tremella fermented product, grape seed fermented material and human stem cell
Growth factor SCGF；
Prepare A phases：Including 5 parts by weight of soybean peptide fermentates are well mixed with 5 parts by weight oat peptide fermentates；
Prepare B phases：Including 10 parts by weight rice bran extracts, 10 parts by weight rice bran fermentates are well mixed；
Prepare C phases：Including by 10 parts by weight ginseng shell fermentates, 10 parts by weight ginseng fermentates, 10 parts by weight people
Ginseng cauline leaf fermentate is well mixed；
A phase, B phase, C phase is well mixed, 0.02 part of human stem cell growth SCGF is added, 35 DEG C of water-baths of constant temperature, stirs
Mix uniformly；Add 5 parts by weight ginkgo fermentates, 5 parts by weight Tremella fermented products, 1 parts by weight grape seed fermented material and 28.98
The deionized water of parts by weight, heat and be well mixed at 70 DEG C, obtain fermentation composition.
Embodiment 2
Soybean Peptide fermentate, oat peptide fermentate, rice bran extract, rice bran fermentate, the fermentation of ginseng shell are prepared respectively
Thing, ginseng fermentate, gen-seng haulms fermentate, ginkgo fermentate, Tremella fermented product, grape seed fermented material and human stem cell
Growth factor SCGF；
Prepare A phases：Including 1 parts by weight of soybean peptide fermentate is well mixed with 10 parts by weight oat peptide fermentates；
Prepare B phases：Including 5 parts by weight rice bran extracts, 15 parts by weight rice bran fermentates are well mixed；
Prepare C phases：Including by 5 parts by weight ginseng shell fermentates, 15 parts by weight ginseng fermentates, 10 parts by weight ginsengs
Cauline leaf fermentate is well mixed；
A phase, B phase, C phase is well mixed, 0.05 part of human stem cell growth SCGF is added, 38 DEG C of water-baths of constant temperature, stirs
Mix uniformly；Add 1 parts by weight ginkgo fermentate, 8 parts by weight Tremella fermented products, 5 parts by weight grape seed fermented materials and 24.95
The deionized water of parts by weight, heat and be well mixed at 65 DEG C, obtain fermentation composition.
Embodiment 3
Soybean Peptide fermentate, oat peptide fermentate, rice bran extract, rice bran fermentate, the fermentation of ginseng shell are prepared respectively
Thing, ginseng fermentate, gen-seng haulms fermentate, ginkgo fermentate, Tremella fermented product, grape seed fermented material and human stem cell
Growth factor SCGF；
Prepare A phases：Including 10 parts by weight of soybean peptide fermentates are well mixed with 1 parts by weight oat peptide fermentate；
Prepare B phases：Including 15 parts by weight rice bran extracts, 5 parts by weight rice bran fermentates are well mixed；
Prepare C phases：Including by 15 parts by weight ginseng shell fermentates, 1 parts by weight ginseng fermentate, 15 parts by weight ginsengs
Cauline leaf fermentate is well mixed；
A phase, B phase, C phase is well mixed, 0.02 part of human stem cell growth SCGF is added, 40 DEG C of water-baths of constant temperature, stirs
Mix uniformly；Add 10 parts by weight ginkgo fermentates, 1 parts by weight Tremella fermented product, 0.5 parts by weight grape seed fermented material and
The deionized water of 26.48 parts by weight, heat and be well mixed at 60 DEG C, obtain fermentation composition.
Embodiment 4
Soybean Peptide fermentate, oat peptide fermentate, rice bran extract, rice bran fermentate, the fermentation of ginseng shell are prepared respectively
Thing, ginseng fermentate, gen-seng haulms fermentate, ginkgo fermentate, Tremella fermented product, grape seed fermented material and human stem cell
Growth factor SCGF；
Prepare A phases：Including 3 parts by weight of soybean peptide fermentates are well mixed with 8 parts by weight oat peptide fermentates；
Prepare B phases：Including 12 parts by weight rice bran extracts, 12 parts by weight rice bran fermentates are well mixed；
Prepare C phases：Including by 1 parts by weight ginseng shell fermentate, 5 parts by weight ginseng fermentates, 12 parts by weight ginsengs
Cauline leaf fermentate is well mixed；
A phase, B phase, C phase is well mixed, 0.01 part of human stem cell growth SCGF is added, 30 DEG C of water-baths of constant temperature, stirs
Mix uniformly；Add 5 parts by weight ginkgo fermentates, 5 parts by weight Tremella fermented products, 2 parts by weight grape seed fermented materials and 34.99
The deionized water of parts by weight, heat and be well mixed at 80 DEG C, obtain fermentation composition.
Embodiment 5
Soybean Peptide fermentate, oat peptide fermentate, rice bran extract, rice bran fermentate, the fermentation of ginseng shell are prepared respectively
Thing, ginseng fermentate, gen-seng haulms fermentate, ginkgo fermentate, Tremella fermented product, grape seed fermented material and human stem cell
Growth factor SCGF；
Prepare A phases：Including 8 parts by weight of soybean peptide fermentates are well mixed with 3 parts by weight oat peptide fermentates；
Prepare B phases：Including 8 parts by weight rice bran extracts, 12 parts by weight rice bran fermentates are well mixed；
Prepare C phases：Including by 5 parts by weight ginseng shell fermentates, 12 parts by weight ginseng fermentates, 1 parts by weight ginseng
Cauline leaf fermentate is well mixed；
A phase, B phase, C phase is well mixed, 0.08 part of human stem cell growth SCGF is added, 35 DEG C of water-baths of constant temperature, stirs
Mix uniformly；Add 8 parts by weight ginkgo fermentates, 8 parts by weight Tremella fermented products, 0.8 parts by weight grape seed fermented material and
34.12 the deionized water of parts by weight, heat and be well mixed at 75 DEG C, obtain fermentation composition.
Embodiment 6-10
Preparation using fermentation composition of the present invention as the mildy wash of raw material
Laureth sodium sulfovinate, laruyl alcohol sodium sulfovinate are mixed with deionized water, 80 DEG C is heated to and stirs to complete
Fully dissolveds, 70 DEG C are cooled to after being incubated 10min, coconut oleoyl amine MEA, glycol distearate is added, stirs to whole dissolvings,
Continue to be cooled to 60 DEG C of addition glycerine, Cocoamidopropyl betaine, the fermentation composition in embodiment 1-5, esters of acrylic acids
Copolymer, citric acid, stir, and add sodium chloride, iodine propilolic alcohol butyl mephenesin Carbamate, stir, be cooled to room temperature,
Obtain the mildy wash of embodiment 6- embodiments 10.
Embodiment 11-15
Preparation using fermentation composition of the present invention as the water class cosmetics of raw material
By allantoin, glycerine, mixed with propylene glycol stirring, 80 DEG C are heated to, is incubated 10 minutes, adds Jojoba wax PEG-
Fermentation composition in 120 esters, beta glucan, dipotassium glycyrrhizinate, tocopherol acetate, embodiment 1-5, stirs.Drop
Temperature is to 50 DEG C；Cremophor RH40 is added, after stirring, adds Bis(hydroxymethyl)imidazolidinyl urea, iodine propilolic alcohol
Butyl mephenesin Carbamate, triethanolamine, stirs, and is cooled to room temperature, obtains the toner of embodiment 11- embodiments 15.
Embodiment 16-20
Preparation using fermentation composition of the present invention as the cream kind cosmetics of raw material
Glycerine, fermentation composition, methyl hydroxybenzoate are heated to 80 DEG C, are incubated 10 minutes；By stearine, Dormant oils,
C10-16 alkyl glucosides, different ethylhexyl palmitate, vaseline, caprylic/capric triglyceride, dimethyl silicone polymer, whale
Wax stearyl alcohol, PEG-25 cetostearyl alcohols, Nipasol sequentially add oil cauldron, are stirred and heated to 80 DEG C, are incubated 10 points of kinds；Emulsification
Pot is preheating to 40 DEG C, vacuumizes, first draws water, then pumps, and opens stirring, and raw material exhausts, homogeneous 3 minutes, is incubated 5 minutes；Add real
Apply the fermentation composition of a 1-5, stirring cooling；50 DEG C are cooled to, adds triethanolamine；Stirring cools to 45 DEG C, add essence,
Bis(hydroxymethyl)imidazolidinyl urea, iodine propilolic alcohol butyl mephenesin Carbamate, stirring is cooled to room temperature, when embodiment 16-20 face
Frost.
1st, anti-oxidation efficacy is tested
Selecting 20 people, average age was 55 one full year of life as tester, mostly dried water deficient skin quality.Subject is daily
Sooner or later face cleaning is carried out using the mildy wash of embodiment 6, altogether using 90 days, once facial skin was tested with skinanalysis apparatus every 15 days
Skin situation.
The use mildy wash of table 1 each index situation of change of skin of face before and after 90 days
| Index | Before use | After use |
| Oil content/% | 12 | 10 |
| Moisture/% | 4 | 8 |
| Elasticity/% | 35 | 42 |
| Collagenous fibres/% | 55 | 62 |
| Pigment/% | 30 | 26 |
Selecting 20 people, average age was 55 one full year of life as tester, mostly dried water deficient skin quality.Subject is daily
The toner of embodiment 11 is used after morning and evening face cleaning, altogether using 90 days, once facial skin was tested with skinanalysis apparatus every 15 days
Skin situation.
The use toner of table 2 each index situation of change of skin of face before and after 90 days
| Index | Before use | After use |
| Oil content/% | 12 | 8 |
| Moisture/% | 4 | 7 |
| Elasticity/% | 35 | 42 |
| Collagenous fibres/% | 55 | 62 |
| Pigment/% | 30 | 26 |
Selecting 20 people, average age was 55 one full year of life as tester, mostly dried water deficient skin quality.Subject is daily
The face cream of embodiment 16 is used after morning and evening face cleaning, altogether using 90 days, a skin of face was tested with skinanalysis apparatus every 15 days
Situation.
The use face cream of table 3 each index situation of change of skin of face before and after 90 days
| Index | Before use | After use |
| Oil content/% | 12 | 8 |
| Moisture/% | 4 | 8 |
| Elasticity/% | 35 | 40 |
| Collagenous fibres/% | 55 | 60 |
| Pigment/% | 30 | 25 |
20 average ages are the tester of 55 one full year of life, after mildy wash of the present invention, toner, face cream, facial skin
The average level of skin indices situation of change is as shown in Table 1-3.As can be seen that the skin of face line after being used continuously 90 days
Reason substantially shoals, and face contour becomes to compact, and absolutely proves the fermentation composition of the present invention and has good anti-aging performance.
2nd, white-skinned face function is tested
Experiment crowd：Totally 60 people, age between 20 to 50 years old, keep original regularity in one's life during experiment, examination
The crowd of testing is divided into four groups, and one group is the dark yellow crowd of blee, and two groups are to be shone caused by face has primary macule, ultraviolet injury
Spot, three groups have the pigmentation after dark sore for face, and four groups have the bounce-back spot produced after multiple whitening failure for face, and pigment sinks
.Subject carries out face cleaning using the mildy wash of embodiment 6 sooner or later daily, and the change of embodiment 11 is used after daily morning and evening face cleaning
Adornment water, subject is daily using the face cream that embodiment 16 is further used after the toner of embodiment 11.
(1) measure of skin complexion：The skin color tester produced using German Courage+Khazaka (CK) company
Colorimeter CL400 determine the measure of target site skin complexion L values, by the change for measuring skin L values before and after product use
Change to assess influence of the product to the bright degree of skin.
The skin complexion L value test results of table 4
| Testing time | First group | Second group | 3rd group | 4th group |
| 0 day | 40 | 55 | 32 | 47 |
| 14 days | 48 | 63 | 40 | 52 |
| 28 days | 52 | 64 | 43 | 53 |
| 35 days | 55 | 65 | 48 | 55 |
| 42 days | 57 | 69 | 52 | 61 |
| 56 days | 69 | 70 | 57 | 70 |
(2) measure of dermal melanin：Using German Courage+Khazaka (CK) company produce dermal melanin and
Melanin value in ferroheme measuring instrument MX18 measure skins, target site dermal melanin measure 5 times, take its average value, pass through survey
Volume production product assess the skin whitening efficacy of the product using the change of front and rear dermal melanin numerical value.
The dermal melanin numerical measuring result of table 5
(3) researcher and research object carry out whitening efficiency assessment：Evaluation index include skin moisturizing, skin splash,
Skin gloss and gloomy skin color.
Table 6 whitening validity (ratio %) test result
| Testing time | First group | Second group | 3rd group | 4th group |
| 14 days | 20 | 25 | 38 | 20 |
| 28 days | 38 | 30 | 33 | 10 |
| 35 days | 58 | 28 | 20 | 8 |
| 42 days | 65 | 25 | 10 | 5 |
| 56 days | 82 | 15 | 5 | 5 |
Test result is as shown in table 4 to table 6, and from table 4, into table 6, data can be seen that using the fermentation containing the present invention
Composition be cosmetics made from raw material after, have obvious whitening effect to the crowd of all kinds of skin quality.
In summary, cosmetics are prepared as raw material using the fermentation composition containing human stem cell growth of the present invention,
It can ensure that obtained cosmetics are entirely free of preservative, artificial color, essence and flavoring agent, mineral oil etc. and have harmful effect to skin
Chemical components, raw material in fermentation composition is substantially all to be extracted from nature, safe and non-stimulating.Obtained cosmetics are not
Only there is good antioxygenic property, can speed up the new life of epidermis, promote the discharge of melanin, play the effect of crease-resistant reparation
Fruit, regain skin and compact fine and smooth, take leave of dim mute coarse, allow skin to recover the health status as youth, and can also play good
Good whitening and moistening effect, and preparation method is simple, has very good application prospect
Fermentation composition of the present invention prepares the usage and dosage of gained cosmetics as raw material, can be according to similar cosmetics
Common usage and dosage use.
Embodiment described above only expresses the several embodiments of the present invention, and its description is more specific and detailed, but simultaneously
Therefore the limitation to the scope of the claims of the present invention can not be interpreted as.It should be pointed out that for one of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the guarantor of the present invention
Protect scope.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.
Claims (10)
1. a kind of fermentation composition containing human stem cell growth, it is characterised in that with the mass parts of the fermentation composition
Number is 100, and the fermentation composition is made up of the component of following parts by weight：1-10 part Soybean Peptide fermentates, 1-10 part oat peptides
Fermentate, 10-20 part rice bran extracts, 10-20 part rice bran fermentates, 5-15 part ginseng shell fermentates, 5-15 part ginsengs
Fermentate, 5-15 part gen-seng haulms fermentates, 1-10 part ginkgo fermentates, 1-10 part Tremella fermented products, 0.5-5 parts grape pip hair
The water of ferment thing, 0.01-0.1 part human stem cell growth SCGF and surplus.
2. fermentation composition according to claim 1, it is characterised in that the Soybean Peptide fermentate and oat peptide hair
Ferment thing is obtained by fermentation of bacillus subtilis Soybean Peptide and oat peptide.
3. fermentation composition according to claim 2, it is characterised in that the oat peptide from oat bran by using
Enzymic degradation extracts to obtain.
4. fermentation composition according to claim 2, it is characterised in that the Soybean Peptide is by the way that pure natural soybean is passed through
Fermentation or enzyme engineering handle to obtain.
5. fermentation composition according to claim 1, it is characterised in that the Tremella fermented product includes tremella polysaccharides, with
The quality of the Tremella fermented product is 100%, and the weight/mass percentage composition of the tremella polysaccharides is not less than 80%.
6. fermentation composition according to claim 1, it is characterised in that the rice bran extract includes inositol, vitamin
B, phytic acid and mineral matter.
7. fermentation composition according to claim 6, it is characterised in that the mineral matter include potassium, magnesium, sodium, calcium, iron,
Selenium and zinc；The vitamin B includes vitamin B2.
8. fermentation composition according to claim 1, it is characterised in that the rice bran fermentate includes the albumen of high activity
Matter and forulic acid.
9. a kind of preparation method of fermentation composition according to claim 1, it is characterised in that the preparation method includes：
Soybean Peptide fermentate, oat peptide fermentate, rice bran extract, rice bran fermentate, ginseng shell fermentate, ginseng are prepared respectively
Fermentate, gen-seng haulms fermentate, ginkgo fermentate, Tremella fermented product, grape seed fermented material and human stem cell growth
SCGF；Soybean Peptide fermentate is well mixed with oat peptide fermentate, A phases are made；Rice bran extract and rice bran fermentate are mixed
Close uniformly obtained B phases；Ginseng shell fermentate, ginseng fermentate are well mixed with gen-seng haulms fermentate, C phases are made；
A phase, B phase, C phase is well mixed, adds the human stem cell growth SCGF, 30 DEG C of -40 DEG C of water-baths of constant temperature, stirring is
It is even；Ginkgo fermentate, Tremella fermented product and grape seed fermented material are added, is heated at 60 DEG C -80 DEG C well mixed.
10. a kind of cosmetics, it is characterised in that the raw material of the cosmetics includes the hair described in claim 1-8 any one
Ferment composition.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711278680.3A CN107837206A (en) | 2017-12-06 | 2017-12-06 | Fermentation composition containing human stem cell growth factor, preparation method and cosmetics |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711278680.3A CN107837206A (en) | 2017-12-06 | 2017-12-06 | Fermentation composition containing human stem cell growth factor, preparation method and cosmetics |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107837206A true CN107837206A (en) | 2018-03-27 |
Family
ID=61664044
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711278680.3A Withdrawn CN107837206A (en) | 2017-12-06 | 2017-12-06 | Fermentation composition containing human stem cell growth factor, preparation method and cosmetics |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107837206A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111544333A (en) * | 2020-05-18 | 2020-08-18 | 广州卡迪莲化妆品科技有限公司 | Polypeptide composition, moisturizing and anti-aging composition and cosmetic |
-
2017
- 2017-12-06 CN CN201711278680.3A patent/CN107837206A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111544333A (en) * | 2020-05-18 | 2020-08-18 | 广州卡迪莲化妆品科技有限公司 | Polypeptide composition, moisturizing and anti-aging composition and cosmetic |
| CN111544333B (en) * | 2020-05-18 | 2021-09-14 | 广州卡迪莲化妆品科技有限公司 | Polypeptide composition, moisturizing and anti-aging composition and cosmetic |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104000756B (en) | Hydrating moistening cosmetic containing rice extract and preparation method thereof | |
| JP2021020887A (en) | Enhanced anti-aging cosmetic composition | |
| CN105250184B (en) | A kind of whitening anti-aging Essence and preparation method thereof | |
| KR101926263B1 (en) | Cosmetic compositions containing fermented extract of Origanum majorana, Corchorus olitorius and Melissa officianalis | |
| CN112494403B (en) | Oat fermentation liquor and preparation method and application thereof | |
| CN109893464A (en) | It is a kind of to block the whitening spot-removing skin care item and preparation method thereof for inhibiting reduction melanin | |
| CN105287341A (en) | Whitening moisture-retention essence and preparation method thereof | |
| CN107823092A (en) | Anti-aging polypeptide composition, preparation method and application thereof | |
| KR101825803B1 (en) | Cosmetic Anti aging composition of mountain-ash's ferment | |
| KR20130088997A (en) | Cosmetic composition for hair growth and restoration from caviar extracts and its fermentation | |
| CN106722999B (en) | Collagen peptide preparation method, low-sugar collagen beauty cake containing collagen peptide preparation method and preparation method | |
| CN107753357A (en) | Toning lotion containing stem cell secretion factors | |
| CN110801411A (en) | Moisturizing, moisturizing and whitening emulsion and preparation method thereof | |
| CN112675084B (en) | Moringa oleifera extract for promoting collagen secretion and application thereof | |
| CN109528536A (en) | A kind of skin care item and preparation method thereof containing choline ellagic acid salt | |
| CN105310972A (en) | Anti-wrinkle skin care product | |
| CN109077971B (en) | A kind of preparation method of fermented soybean milk and its application in cosmetics | |
| CN111603404A (en) | Skin care essence containing cell extracting solution and preparation method thereof | |
| CN107837206A (en) | Fermentation composition containing human stem cell growth factor, preparation method and cosmetics | |
| FR2928549A1 (en) | USE OF AN EXTRACT OF BRASSOCATTLEYA MARCELLA KOSS ORCHIDEE AS AN AGENT TO PREVENT OR DELAY THE APPEARANCE OF SIGNS OF SKIN AGING | |
| CN110522662A (en) | A kind of ganoderma lucidum face cream and preparation method thereof | |
| CN113876671A (en) | Anti-aging composition containing rape bud extract and preparation method thereof | |
| CN115414289A (en) | Cordyceps flower cosmetics and preparation method thereof | |
| KR101806007B1 (en) | Manufacturing method of cosmetic composition containing nano particle natural materials and cosmetic composition manufactured by the same | |
| CN114557938A (en) | Composition with brightening, compacting and anti-wrinkle functions and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20180327 |