KR20130088997A - Cosmetic composition for hair growth and restoration from caviar extracts and its fermentation - Google Patents

Abstract

PURPOSE: A cosmetic composition containing a caviar extract and a fermented caviar extract as active ingredients is provided to promote hair growth, to generate hair, to preventing alopecia, and to improve scalp. CONSTITUTION: A composition for preventing alopecia, promoting hair growth, and improving scalp contains a fermented caviar extract. The composition also contains a caviar extract. The composition is prepared by mixing the caviar extract, Bacillus subtilis, and fermentation microorganisms and fermenting the mixture.

Description

Cosmetic composition for hair growth, hair growth, hair loss prevention and scalp improvement using raw caviar extract and fermentation extract {Cosmetic composition for hair growth and restoration from Caviar extracts and its fermentation}

The present invention relates to hair growth, hair growth, hair loss prevention composition containing a raw caviar extract and fermented extract as an active ingredient, and more particularly to a cosmetic composition for improving the scalp having a biological activity and excellent skin vitality of the scalp.

Caviar, the sturgeon's egg, contains essential fatty acids, such as omega-3, which tend to grow short of age, nourish and moisturize the skin, protect skin from aging and dryness, and provide excellent scalp care. Have In addition, the effective factor in the protein contained in the caviar protects the irritation from the outside, it has a variety of moisturizing and anti-aging ingredients to play a role in forming a smooth, supple skin. Raw caviar fermentation extract produced through fermentation process using Bacillus subtilis, lactobacillus and yeast protects the scalp that is easy to be sensitive by supplying nutrition to the living body, and also improves and maintains the elasticity of the skin. In addition to solving the factors, it is provided as a cosmetic for improving scalp that has excellent functions in hair growth, hair growth, hair growth promotion, hair regeneration and hair loss prevention.

While the development of new compositions to overcome the high and rare high value natural raw materials has emerged as a global concern, the caviar, the sturgeon egg, contains a large amount of low-fat, high-protein nutrients, an essential nutrient for humans. Creation potential is expected.

Sturgeon is the largest freshwater fish, boneless and cartilaginous, and appeared only between 450 and 350 million years ago and is the oldest and only surviving fish. Sturgeon is mostly distributed in Russia's Caspian Sea, Narrow Sea, Black Sea, Baltic Sea, etc., and is a anadromous fish whose mother grows in the sea, climbs up the river to spawn, spawns upstream, and the young descends back into the sea to mature. Until it grows. Spawning is not annual, spawns only a few times in life. Therefore, the artificial hatching technology has been developed, and the breeding method of the mass production method using the same has become possible. Caviar, the sturgeon's egg, is mainly produced from three species of Beluga, Ossetra and Sevruga sharks, which are collected before the spawning season.

Caviar is composed of nutrients such as proteins, fats, sugars, vitamins, minerals and the like, and has been reported as a perfect nutritional food with low calories and high protein content. Russians use caviar for recovery of surgical patients and prevent and treat rickets in children because of their high protein content and ease of digestion. The protein contained in caviar is a stable balance of amino acids such as water-soluble glutamic acid, aspartic acid, leucine, isoleucine, lysine, alanine, serine and arginine. Because of this feature, proteins of caviar have a strong affinity with skin and hair, and amino acids are found to act as essential components of cellular metabolism. Caviar protein structure is similar to the natural skin and plant material of any species in the world compared to the human skin structure is known as an excellent substance that provides skin absorption and nutritional supplements. In particular, unlike other existing natural ingredients are excellent absorption ability to penetrate the skin, and is said to have an excellent effect on the scalp recovery against tired and fatigued skin aging and hair loss phenomenon.

Raw caviar fermentation extract using Bacillus subtilis, lactic acid bacteria, yeast of the present invention can maintain and preserve the excellent quality and efficacy of caviar more stably. These characteristics suggest that protein of caviar component has close affinity with scalp and acts as an essential cell metabolic factor. In addition, the role of regenerating the lipid membrane of the dermis is mostly fat component, caviar contains both fat-soluble and water-soluble vitamin elements. In other words, the raw caviar extract and fermented extract of the present invention have a great affinity with the scalp related to skin regeneration, and can be used as a leading scalp cosmetics to relieve anxiety and extreme stress caused by hair loss.

Hair loss (Alopecia) refers to a condition in which the hair is not normally present in the hair, and generally means that the hair of the scalp (coarse hair) is missing. The mechanism of hair loss is not yet known, but in general, excessive sebum production due to abnormal endocrine system such as hormonal imbalance, circulatory system such as autonomic nervous system, blood circulation disorder, malnutrition of hair root, allergy, bacterial infection and genetic factors, Disease status, aging, etc. have been presumed to be the main causes, and in recent years, the number of patients with hair loss is increasing even before age 30 due to mental stress, air pollution, and food from complex environments. Hair loss can be divided into two types: clinically formed scars and non-formed hair loss. Hair loss that forms scars does not cause hair regeneration due to destruction of hair follicles, whereas hair loss that does not form scars causes hair follicles to remain. The hair regenerates after the site disappears. There is feminine hair loss, but the frequency and severity are more common in male hair loss. This difference in hair loss between men and women has been found to be due to androgen and estrogen, the sex hormones that determine hair changes. In women, hair loss can occur when the female hormone estrogen decreases. Androgenetic alopecia in men is a phenomenon in which the effects of estrogen are suppressed due to excessive secretion of androgens. The most common cause is compound action. In order for masculine alopecia to occur, hair loss genes must be present, but hair loss genes do not all cause hair loss, and even if there are no hair loss genes, hair loss begins because some hair follicles shrink when they age. Hair loss also begins at different times, and is a complex process that depends on the combination of the individual's genes and the concentration of the male hormone.

Scalp aging can be caused by the activation of free radicals due to ultraviolet rays, inflammation, environmental factors, age increase, etc. If this condition is exacerbated, antioxidant defenses in vivo are destroyed, hair follicle cells and tissues are damaged, and aging can be prevented. Can be promoted. As a result, lipids, proteins, polysaccharides, and nucleic acids, which are major constituents of the skin, are oxidized to destroy scalp cells and tissues, resulting in hair loss. Therefore, by eliminating the free radicals to protect the cell membrane of the hair follicle cells, the already damaged hair follicle cells must be regenerated and proliferated by active metabolism to recover quickly to maintain a healthy scalp.

Androgenetic alopecia, the most representative of the various types of alopecia, is a common alopecia caused by genetic predisposition and testosterone. Hair loss is caused by dihydrotestosterone (DHT) produced by 5α-reductase from testosteron. Gradual miniaturization of hair follicles is characteristic and clinically, due to increasing proportion of resting hair and decreasing growth period, clinically, the frontal and head of the scalp become thinner and the forehead retreats. In the past, androgenetic alopecia was regarded as a normal aging phenomenon, and Caucasians showed androgenetic alopecia in more than 50% of adult males, but Koreans had a relatively low incidence, such as 10-20% lower by age than Caucasians. However, in recent years, the occurrence of androgenetic alopecia has increased significantly in the Orient including Korea. Accordingly, interest in androgenetic alopecia and the demand for treatment are increasing rapidly. To date, oral finasteride and minoxidil coatings have been approved by the US Food and Drug Administration (FDA) for the treatment of androgenetic alopecia. Among these, minoxidil is a piperidinopyrimidine derivative and is the most widely used hair repellent for the treatment of androgenetic alopecia. The mechanism of action of minoxidil on hair growth is not clear, but it acts on peripheral blood vessels, induces local blood flow and directly acts on hair follicle epithelium, induces hair growth, activates hair follicles by the potassium pathway and immune response It is believed to act as a regulator of and promote hair growth. The therapeutic effects of existing drugs, including minoxidil, are not always satisfactory, and many new drugs are being developed, but many have not been scientifically validated for their effectiveness. need.

Raw caviar extract and fermented extract of the present invention is a composition for hair growth and hair growth regeneration, a composition for improving the scalp having an effect of hair loss treatment similar to the existing minoxidil formulation. Raw caviar extracts and fermented extracts are expected to be more effective than minoxidil alone because they have an anti-androgen effect that enhances skin physiological activity and absorption, delays evaporation in the scalp, thereby long-lasting in hair follicles, and enhances local action.

An object of the present invention relates to a cosmetic composition containing an active ingredient of the raw caviar extract and fermented extract, to provide a cosmetic composition for improving the scalp exhibiting excellent functions in hair growth, hair growth, hair growth promotion, hair regeneration, hair loss prevention will be. More specifically, by using a fermentation product using multiple complex strains of caviar extract and raw caviar without undergoing a separate salting process, the scalp improvement cosmetics using a stabilized formulation further increases the effect of the active ingredient. For the purpose of providing

In the method of caviar extraction according to the present invention, the normal caviar extraction method without the salting process and the salted caviar general extraction method with the salting process were used. The extraction method comprises the steps of: (a) mixing the water heated to 80 ℃ to the raw caviar, and stirring; (b) naturally cooling to room temperature. The raw caviar general extract obtained by the above method can obtain the raw caviar fermentation extract through a fermentation process. In more detail, the fermentation method includes the steps of: (a) fermenting fresh caviar using Bacillus subtilis; (b) fermenting fresh caviar using lactic acid bacteria and yeast; And (C) characterized in that it comprises a step of mixing fermentation of one or two or more of Bacillus subtilis and complex microorganisms (lactic acid bacteria, yeast) in the raw caviar. The fermentation product obtained by the above method may be subjected to the step of aging, and the fermentation product obtained therefrom or the concentrate obtained by partially or totally reducing the concentration and freeze-drying the fermentation product, or the main effect contained in the fermentation product and the concentrate. It contains the chemicals that exert themselves.

The fermentation process is characterized in that the fermentation process is one or two or more of Bacillus subtilis and fermentation microorganisms (lactic acid bacteria, yeast). Adjusting the medium to pH 4.0-5.0 to about 5.0 to 7.5 and, respectively, ⁷ to 10 Bacillus subtilis, lactic acid bacteria, yeast pre-incubation - 10 ⁸ CFU / mL was added to a concentration, 25 ℃ to 50 ℃ in a temperature range of 18 hours to 120 Time incubation.

The cosmetic composition according to the present invention may contain, in addition to the above extracts and fermented products, other ingredients which may give a synergistic effect to the main effect without impairing the main effect of the present invention. The raw caviar extract and fermented extract may be present in the composition at 0.001 to 95% by weight based on the total lyophilized weight. Hereinafter, the present invention will be described in detail with reference to Examples, Comparative Examples, and Experimental Examples. However, the present invention is not limited thereto. In the present invention, in order to confirm the hair growth, hair growth, hair growth promotion, hair growth regeneration, hair loss prevention effect of the composition containing the caviar extract and fermented extracts, hair follicle experiments, mouse hair growth promoting effect, hair growth experiments targeting hair loss patients Antioxidative and inflammatory cytokine expression inhibition experiments confirmed the effect of scalp improvement through inhibition of free radical production by external environment and cell membrane protection of hair follicle cells.

As described above, the extract of the raw caviar extract and fermentation extract according to the present invention, by inoculating one or two or more of Bacillus subtilis and fermented microorganisms (lactic acid bacteria, yeast) without undergoing a separate salting process It is characterized by using a single or mixed fermentation method. Through this, it is possible to effectively reduce contamination by various bacteria and poor fermentation odor without destroying the active ingredient. The cosmetic composition for improving the scalp containing the omega-3 complex component extracted from the raw caviar of the present invention has been found to have excellent antioxidant, anti-inflammatory and skin regeneration promoting effects, as well as activating the scalp, preventing skin aging and improving wrinkles. Is excellent. Due to this effect, the raw caviar extract and fermented extract obtained by the above method can be usefully used in cosmetics, food, medicine, etc. for the purpose of promoting anti-aging and scalp regeneration activity, excellent hair growth and hair growth external preparations.

<Example 1> Preparation of fresh caviar Bacillus subtilis

Distilled water of 80 占 폚 in an amount of 10 times that of raw caviar is mixed and stirred to dissolve, and the obtained extract is naturally cooled to room temperature. The culture solution pH was adjusted to 5.0-7.5, and precultured Bacillus subtilis was added to a concentration of 10 ⁷ -10 ⁸ CFU / mL, and then cultured at 25 ° C to 50 ° C for 18 hours to 120 hours. The fermentation was terminated by heating at 80 ° C for 10 minutes. The precipitate was removed using a centrifuge and the supernatant was collected, or the filtered supernatant was used.

<Example 2> Preparation of raw caviar fermented microorganisms (lactic acid bacteria and yeast) fermented product

Distilled water of 80 占 폚 in an amount of 10 times that of raw caviar is mixed and stirred to dissolve, and the obtained extract is naturally cooled to room temperature. The pH of the culture was adjusted to 4.0-5.0 to 5.0-7.5, and the pre-cultured lactic acid bacteria and yeast were added to the resulting solution at a concentration of 10 ⁷ -10 ⁸ CFU / mL, and cultured at 25 ° C to 50 ° C for 18 to 120 hours Respectively. In order to terminate the fermentation, heat was applied at 80 ° C. for 10 minutes to induce loss of function of lactic acid bacteria and yeast. The precipitate was removed using a centrifuge and the supernatant was collected, or the filtered supernatant was used.

<Example 3> Preparation of raw caviar complex fermentation product

Distilled water of 80 占 폚 in an amount of 10 times that of raw caviar is mixed and stirred to dissolve, and the obtained extract is naturally cooled to room temperature. Adjust the culture solution pH to 4.0-5.0 to 5.0-7.5, add pre-cultured Bacillus subtilis, lactic acid bacteria, and yeast so as to have a concentration of 10 ⁷ -10 ⁸ CFU / mL, respectively, and then 18 to 18 hours at a temperature of 25 ℃ to 50 ℃ Incubated for 120 hours. In order to terminate the fermentation, heat was applied at 80 ° C. for 10 minutes to induce functional defects of Bacillus subtilis, lactic acid bacteria and yeast. The precipitate was removed using a centrifuge and the supernatant was collected, or the filtered supernatant was used.

<Comparative Example 1> Preparation of fresh caviar general extract

The raw caviar general extract used as a control was obtained through the same extraction process without the fermentation process. That is, distilled water at 80 占 폚 in an amount of 10 times that of raw caviar is mixed and stirred to dissolve, and the obtained extract is naturally cooled to room temperature. The precipitate was removed using a centrifuge, the supernatant was collected, or the filtered supernatant was used as a general extract of raw caviar.

<Comparative Example 2> Preparation of Salted Caviar General Extract

The salted caviar extract used as a control was obtained through the same extraction process after adding only the salting process without the fermentation process. That is, 5% salt is sprinkled with fresh caviar, and 10-fold amount of 80 ° C distilled water is mixed and stirred to dissolve, and then the obtained salt extract is naturally cooled to room temperature. The precipitate was removed using a centrifuge and the supernatant was collected or the filtered supernatant was used as the salt extract of the caviar.

<Experimental example 1> Antioxidation effect measurement using DPPH method

In order to measure the antioxidant activity of the raw caviar fermented extract, DPPH (2.2-diphenyl-1-picryl hydrezyl) radical scavenging ability was used.

500 uM DPPH (2.2-diphenyl-1-picryl hydrezyl) solution was dissolved in ethanol and filtered. The prepared DPPH solution and the sample solution were mixed at a ratio of 1: 1 and mixed vigorously. The mixture was allowed to stand at room temperature for 20 minutes. After measuring the absorbance at 540 nm using a Microplate reader (Chromate, USA), DPPH radical removal rate (%) was calculated through the following equation (1). At this time, 1 mM ascorbic acid was used as a positive control to remove radicals.

As shown in Table 1, the general caviar extract of Comparative Example 1 was confirmed to increase the antioxidant activity compared to the general extract after the salting process of Comparative Example 2. In addition, when comparing the raw caviar fermentation extracts of Examples 1 to 3, Example 1 and Example 2 showed an antioxidant activity at a similar level, while fermentation using a mixed strain of Bacillus subtilis, lactic acid bacteria, yeast of Example 3 Water was found to have stronger antioxidant capacity than each fermentation product.

DPPH radical scavenging (%) = (A-B) / A x 100

Where A is the absorbance of the blank test solution and B is the absorbance of the test solution

Table 1. Antioxidant Activity of Raw Caviar Extracts and Fermented Extracts

<Experimental example 2> Inhibitory effect of inflammatory cytokine expression by ultraviolet irradiation

In this experimental example, in order to evaluate the inhibitory effect of inflammatory cytokine expression expressed by ultraviolet irradiation of the raw caviar extract and the fermented extract obtained in the Examples and Comparative Examples, the fibroblasts isolated from the epidermal tissue of human 24- 5 x 104 pieces were placed in a well test plate and attached for 24 hours. Each well was washed once with PBS and 500 μl of PBS was added to each well. The fibroblasts were irradiated with 10mJ / cm 2 of UV light using an ultraviolet B (UVB) lamp (Model: TL 20 / 40W, UV B 12.7W, Hansung Ultraviolet, Korea). Was added. The samples were treated and incubated for 5 hours. 150 µl of the culture supernatant was taken to quantify IL-1α to determine the inhibitory effect of inflammatory cytokine expression of the fresh caviar extract and the fermented extract. The amount of IL-1α was quantified using an enzyme-linked immunosorbent assay, and the production rate of IL-1α was calculated through Equation 2.

Inhibitory rate of inflammatory cytokine expression (%) = [1- (St-Bo) / (Bt-Bo)] X 100

Bo: IL-1α production amount of wells not irradiated with UV

Bt: IL-1α Production of Wells Not Irradiated with UV Light

St: Production of IL-1α in Wells Treated by Ultraviolet Irradiation

As a result, the general extract without the salting process of Comparative Example 1 was able to confirm the inhibition of inflammatory cytokine expression compared to the general extracts after the salting process of Comparative Example 2, and the fermented extract was inflammatory cytokine by ultraviolet rays compared to the general extract. It can be seen that it effectively protects the production of IL-1α (Table 2).

Table 2. Inhibitory Effects of Raw Caviar Extracts and Fermented Extracts on Inflammatory Cytokine Expression

<Experiment 3> Mouse hair growth effect test

This Experimental Example is a 30% ethanol solution containing 5% of caviar general extracts (Comparative Examples 1 to 2) and raw caviar fermentation extracts (Examples 1 to 3) obtained in Examples and Comparative Examples. Was prepared and used for testing. The hair growth effect test was performed using 47-53 days old mouse (ICR) to remove the back hairs, and the back parts were clean and 10 rats were used for each material group. 100 ml per subject was applied. The length of hair and the degree of hair growth over time were compared by adding scores according to the degree of restoration after hair removal. In order to compare the degree of hair growth, as a control, 30% alcohol solution was applied to each individual to observe the growth state. As a result, it was confirmed that the excellent hair growth promoting effect in the raw caviar extract and fermented extract (Table 3).

Table 3. Effects of Raw Caviar Extracts and Fermented Extracts on Mouse Hair

Experimental Example 4 Evaluation of Activity of Hair Follicle Cells

Cell lines were obtained from human dermal papilla cells purchased from SCIENCELL. Cell culture was incubated in a 5% CO2 incubator using Mesenchymal Cell Medium.

MTT assay was performed to determine the effect of promoting growth of dermal papilla cells of the hair growth and hair growth compositions of Examples and Comparative Examples. MTT assay was performed in the following manner. First, human dermal papilla cells pre-cultured in 100 dishes were removed using trypsin, and the cells were counted using a hemochromometer (Hemacytometer) and adjusted to 10 ⁵ cell / ml.

100 μl of the dermal papilla cell solution (10 ⁴ cells per Well) was put into a 96 well plate and incubated for 1 day. After diluting the hair growth and hair growth composition samples prepared in Examples and Comparative Examples to 2% in the mesenchymal cell medium, each sample was added to 96 wells containing cells cultured for 1 day to increase the first concentration of each sample. It was made to be 1%. 100 µl of the 96 well sample solution was diluted by 1/2 to dilution to 18 wells per sample so that the treatment concentration of the hair growth and hair growth compositions of Examples and Comparative Examples was 0.001%. 96 wells of the samples were incubated for 1 day at 37 ° C. and 5% CO 2, followed by 0.1 mg (20 μl of 5 mg / mL) of MTT (3- (4,5-dimethylthiazol-2-yl) -2,5 -diphenyl-tetrazolium bromide) solution was added to all wells. After incubating for 4 hours at 37 ° C, all of the medium was removed. 200 μl of DMSO was added to each well so that the resulting formazan salt was completely dissolved. After measuring the absorbance at 492 nm with a microplate reader, the extent of growth of dermal papilla cells was determined.

As a result, the raw caviar fermentation extract showed much higher activity of the hair follicle cells than the general extracts of Comparative Examples 1 to 2, and the raw caviar complex fermentation showed the activity of the hair follicle cells above the level of minoxidil used as a positive control. Was excellent (Table 4).

Table 4. Activity of hair follicle cells of fresh caviar extract and fermented extract

<Experimental example 5> Hair growth experiment for hair loss patient

In order to determine the effect on the hair loss patient, a clinical trial was conducted on the hair growth and hair growth compositions of Examples and Comparative Examples in patients with alopecia.

The subjects were male and female alopecia among adult men and women over 20 years old. The test was carried out by spraying two to three times a day to the hair loss site for each of the hair growth and hair growth compositions of Examples and Comparative Examples, and evaluated after 60 days and observed the hair growth state of the application site to determine the effect. Was evaluated in five stages according to the evaluation scale of Table 5 for photographic findings and improvement of subjective symptoms. The results are shown in Table 6 below.

Table 5. Criteria for testing hair growth improvement for hair loss patients

Table 6. Improvement of Hair Growth of Raw Caviar Extracts and Fermented Extracts

As shown in the results of Table 6, compared to the general caviar extracts of Comparative Examples 1 to 2, the hair loss prevention, hair growth, and hair growth effects were significantly increased in the raw caviar fermentation extract through the fermentation process. As described above, the synergistic effect of the extracts fermented with Bacillus subtilis or lactic acid bacteria and yeast was superior to that of conventional minoxidil. In addition, no adverse effects were found in all subjects during the trial period, indicating that they are safe for humans.

Claims (11)

A composition for hair growth, hair growth, hair loss prevention, scalp improvement containing raw caviar fermented extract. Hair growth, hair growth, hair loss prevention, scalp improvement composition containing the raw caviar extract. The composition for hair regrowth, hair growth, hair loss prevention, scalp improvement according to claim 1, wherein the raw caviar extract is fermented with Bacillus subtilis and fermented microorganisms. The composition for hair growth, hair growth, hair loss prevention, and scalp improvement according to claim 1, which is Bacillus subtilis. The method of claim 1, wherein the fermentation microorganism is one or more selected from the group consisting of lactic acid bacteria, yeast and mixed strains of hair growth, hair growth, hair loss prevention, scalp improvement composition. The composition according to claim 1, wherein the composition contains 0.001 to 95% by weight based on the total weight of the raw caviar extract and the fermented extract. The method of claim 1, wherein the raw caviar extract and fermented extract containing or each of the active ingredients, characterized in that hair growth, hair growth, hair loss prevention, scalp improvement cosmetics, pharmaceutical products, external skin composition. According to claim 1, wherein the composition for the hair growth, hair growth, hair loss prevention, scalp improvement in the formulation of nanocapsules, microcapsules, nanosomes, etc. containing the raw caviar fermentation. The skin lotion, gel, lotion, essence, ointment, cream, patch, spray, tablet, pill, syrup, powder, oil-in-water (O / W) type or water-in-oil (W / O) type Characterized by being formulated, hair growth, hair growth, hair loss prevention, scalp improvement composition containing the raw caviar extract and fermented extract. A composition according to claim 1 which is further formulated into a unit dosage form further containing a pharmaceutically or cosmetically acceptable carrier or additive. Hair growth, hair growth, hair loss prevention, scalp improvement composition characterized in that the oral formulation.