JP2016088933A - Age production inhibitor and application thereof - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an AGE production inhibitor related to particularly ED and the care of the scalp/hair among evils caused by AGE produced and accumulated as a result of the in vivo glycation reaction of collagen, elastin, and the like; and to provide applications thereof.SOLUTION: According to the invention, by applying an external or oral formulation containing a particular AGE production inhibitor as an active ingredient, and furthermore by applying an optimal formulation designed by adding a particular AGE production inhibitor in a composition consisting of collagen and/or collagen peptide as a main component and at least one kind selected from elastin, hyaluronic acid, and chondroitin sulfate, ED improvement and scalp function improvement (hair loss prevention/white hair prevention) are achieved.SELECTED DRAWING: None

Description

The present invention relates to an AGEs production inhibitor that focuses on glycation reaction of in vivo proteins and uses thereof.
More specifically, it is an invention related to ED (erection dysfunction), hair loss due to scalp damage, and hair care and eyelash care, which is difficult to improve, not just as an anti-aging application, and its application form as an anti-glycation agent In addition to local things, they constitute systemic care and methods including food and drink forms.

  While we have a permanent desire for mankind to “keep on being youthful forever”, what is called “anti-aging medicine”, so-called anti-aging, was born, and the medical system for overcoming the aging process and its causes has developed. It was.

Recently, a saccharification reaction has attracted attention as a risk factor for aging. The saccharification reaction means that sugar and protein (amino acids) chemically react non-enzymatically, and the generic name of substances generated by the chemical reaction is generally called AGEs (Advanced Glication End Products) It is.

  Even in healthy individuals, protein glycation occurs. The glycation is accelerated by ultraviolet rays or aging, and a final saccharification reaction product (hereinafter simply referred to as “AGEs”) is produced and accumulated. It is a risk factor that cannot be overlooked by diseases associated with recent lifestyle changes and dietary habits. For example, people with diabetes are particularly at high risk and AGEs are likely to accumulate, which accelerates various aging phenomena.

AGEs are accompanied by sclerosis and denaturation of proteins, and their progress brings about a decrease in the function of living tissues, and there are concerns about various effects on health.
Moreover, since proteins are present in body tissues such as the brain, organs, skin, scalp, scalp, nails, blood, etc., naturally, the effects of AGEs production / accumulation are systemic.

Collagen, which is a kind of protein, occupies about 30% of body weight, 40% of which is in the skin (including eyelid skin) and 10-20% is in bone and cartilage. In addition, collagen is also present in blood vessels, scalp, and organs, and saccharification occurs at each of these sites.

  Elastin, which is one of the important proteins in the body like collagen, is a fiber component that brings elasticity to keep collagen in a mesh form, and plays a role in giving elasticity to the skin.

Therefore, both collagen and elastin are directly affected by AGE production. The effects of this AGE production are specifically as follows in the skin, blood vessels and scalp.

That is, in the skin (including eyelid skin, which may be used synonymously hereinafter), glycation of proteins such as collagen protein and elastin fiber occurs, causing a biological reaction that destroys the structure of the collagen fibers that hold the skin firm. Is done. An enzyme that prevents collagen synthesis (collagenase) is also activated. As a result, the skin loses its normal elasticity, changes its color to a state free of firmness and gloss, and waste products accumulate in the skin cells. As a result, the cause of so-called yellowing and darkening is caused, and the transparency of the skin is lost.
In addition, it is often caused by lack of nutritional balance that causes rough skin during dieting, but if you continue unreasonable diet, the stratum corneum that has a barrier function will be damaged and moisture retention will be lost, Decrease in elasticity is accelerated by the effect of saccharification.
At the heel part, so-called eye lashes stand out, and the eyelashes lose their elasticity and elasticity.

AGEs in blood have high affinity and adhesion to blood vessel cells and are likely to accumulate in the blood vessel wall. When AGEs adhere to the blood vessel wall, active oxygen is generated, and as a result of oxidizing NO (nitrogen monoxide), blood flow deteriorates. In relation to diseases such as ED, it becomes difficult to carry blood necessary for erection. It is also a phenomenon that occurs particularly when suffering from diabetes. The human penis is anatomically composed of three cylindrical parts, most of which are composed of collagen and elastin. In the erectile tissue of the cavernous body of the penis, collagen fibers and elastin fibers are one of the important components other than blood vessels and blood, and the production of AGEs has an adverse effect on the erectile tissue. Therefore, to maintain and restore male sexual function, it is extremely important to prevent the influence of AGEs on the blood vessels and the erectile tissue of the corpus cavernosum.
One problem that has become more serious in recent years is infertility treatment. Infertility treatment has different factors for men and women, so efforts for treatment are being made individually, but in the case of men, ED is often a factor, and in that respect the point of anti-glycation is Very meaningful.
On the other hand, saccharification is a direct factor for women. That is, saccharification degrades the ovum, and ovulation becomes impossible when the degree of deterioration becomes significant. In any case, anti-glycation is a common key for male and female infertility treatment.

The scalp has a dermis layer as well as the skin. When AGEs cross-linkage occurs in the dermis layer of the scalp, or when AGEs accumulate due to glycation of the main component of hair, keratin (one of proteins), hair loss, cut hair, and gray hair increase.
In addition, the elasticity of the scalp is generated by elastin, hyaluronic acid, chondroitin sulfate, etc. present in the dermis layer.

The effect on hair is also great, and as the glycation of collagen and elastin present in the dermis layer progresses, the dermis layer hardens, causing adverse effects on secretory glands, hair follicles, blood vessels and the like. As a result, the vitality of making hair declines, and the hair quality is further affected by the loss of elasticity and gloss. The hair papilla located at the root of the hair roots controls the hair growth, but the hair matrix cells divide and keratinize into hair after receiving a stimulus from the hair papilla, and extend from the scalp. Here, when AGEs accumulate in the hair root, the functions of the hair papilla and hair matrix cells are limited. As a result, hair cannot grow sufficiently and hair loss occurs. Eyelashes can be regarded as body hair that grows at the ends of the eyelids, but as with hair, protein degradation such as collagen and elastin occurs due to the effect of glycation on the eyelid skin, and the elasticity and elasticity of the eyelashes disappear, The regeneration cycle is also reduced.

In addition, there are melanocytes adjacent to the hair matrix cells, and when the hair matrix cells divide, they take in melanin pigment from the melanocytes and become black or brown hair. However, when AGEs accumulate in melanocytes, they can supply melanin sufficiently. It disappears and causes gray hair.

  Thus, from the viewpoint of scalp, hair and eyelash care, anti-glycation (saccharification measures), that is, AGEs production suppression measures are attracting attention.

  As for collagen, when it is young, collagen synthase is actively secreted, so abundant collagen is produced and the normal condition of skin quality, blood vessels, and scalp is maintained, but collagen synthase decreases with age. AGE production proceeds. If such a stage further progresses, local care such as so-called UV countermeasures and moisturizing countermeasures is no longer sufficient, and systemic care including eating and drinking becomes essential. The same applies to elastin.

Conventional techniques existed from the viewpoint of active intake of collagen and promotion of synthesis. For example, Patent Document 1 discloses that by ingesting a large amount of collagen protein or a hydrolyzate thereof, biosynthesis of living collagen can be promoted and various diseases associated with a decrease in metabolism of living collagen can be improved. Yes. In Patent Document 2, Tochu or its extract and ginseng or its extract are essential components, and skin turnover by selectively selecting from deoxyribonucleic acid, chondroitin sulfate, collagen or its hydrolyzate and pearl extract. A skin activator that is effective for improvement is disclosed. Patent Document 3 discloses that an oral hair-restoring agent containing macombu temporary root, amateur, ginseng and collagen can promote the function of hair matrix cells and exhibit a hair-growing effect. Patent Document 4 discloses a collagen synthesis promoter composition that activates metabolism of living tissue by utilizing a specific plant and enhancing the ability to promote collagen synthesis in the living body. However, these are not drastic measures focusing on the saccharification reaction as in the present invention, and the obtained effects are not sufficient.

Regarding ED, there are many unclear points in its cause. There are also many problems with therapeutic agents for improving ED, and there are many drugs that have particularly strong side effects and are threatened to life. Drugs for reducing side effects have also been developed, but are still unresolved areas. For example, although certain effects are recognized by drug administration as exemplified in Patent Documents 5 to 7, the problem of side effects still remains, and it is difficult to use continuously in daily life. Moreover, these do not pay attention to the improvement by the AGEs production inhibitor which this invention aims at.

Application examples of AGEs production inhibitors include, for example, Patent Documents 8 to 10. Patent documents 8 and 10 disclose pharmaceutical uses. Patent Document 9 discloses a solution for improving skin quality such as wrinkles, spots, dullness, and tarmi mainly from the viewpoint of cosmetic aging, especially dermatology. However, none of these are applied to ED improvement and a hair restorer for scalp and hair from the viewpoint of anti-glycation.

Japanese Patent Laid-Open No. 7-278012 JP-A-9-67262 JP 2013-116887 A JP 2005-255527 A JP-A-10-114657 JP 2002-114681 A Special table 2010-528108 gazette JP 2009-96773 A JP 2013-139392 A JP 2014-37399 A

  In view of the above-mentioned problems, the object of the present invention is, in particular, for the care of ED, scalp / hair and eyelashes among adverse effects caused by AGEs produced and accumulated as a result of in vivo saccharification reactions such as collagen and elastin. It is in providing the AGEs production inhibitor which concerns, and its use.

  As a result of diligent studies to solve the above problems, the present inventor aims to prevent glycation of these proteins in vivo while supplementing essential collagen, elastin, hyaluronic acid and chondroitin sulfate in tissues in vivo. Providing a composition combined with an AGEs production inhibitor, or providing an optimal method for its use, to prevent decline in collagen function, etc., and to maintain normal male functions and functions such as scalp and hair The headline and the present invention were completed.

  An important factor in developing hair preparations including ED improving agents and scalp / hair growth is the effect of aging. In order to prevent deterioration of in vivo functions of collagen and elastin due to aging, it is necessary to consider from two aspects. Quantitative supplementation from the outside and internal care in in vivo tissues.

In the former, it is necessary to supplement essential components in the body. Especially, it is important to supplement collagen and elastin which are related to tissue elasticity, and hyaluronic acid and chondroitin sulfate, which can sufficiently exert moisture retention. It is. In the present invention, collagen or collagen peptide is an essential component, and elastin, hyaluronic acid and chondroitin sulfate are combined as a supplement component.

The latter is to suppress the production of AGEs in order to prevent the decrease of collagen and elastin after the proteins present in the living body, particularly the influence of saccharification reaction. Here, as an AGEs production inhibitor, in view of uses such as ED improvement, scalp care (hair function improvement / hair loss and white hair prevention) and eyelash care, AGEs production inhibitory effect from among materials having blood flow and nourishing tonic effect After being screened variously, it was found as suitable and used for the present invention. That is, one or more selected from Ashwagandha, Epimedium, Panax ginseng, Guarana, Black Turmeric, Tribulus, Tonkat Ali, Garlic, Pueraria, Maca, Muirapuama, Moringa, Meshimakobu and Royal Jelly have been used as active ingredients. These are all novel AGEs production inhibitors.

Furthermore, in the process of further testing, millet was found as a material for activating these AGEs production inhibitory effects, and the present invention was completed.

That is, according to this invention, the AGEs production inhibitor as described in the following (1) thru | or (14) and its use are provided.

(1) An AGEs production inhibitor comprising as an active ingredient at least one selected from Ashwagandha, Ikarisou, Panax ginseng, Garana, Black turmeric, Tribulus, Tonkat Ali, Garlic, Pueraria, Maca, Muirapuama, Moringa, Meshimakobu, and Royal Jelly.
(2) The AGEs production inhibitor according to (1), wherein millet is added as an activator for inhibiting AGEs production.
(3) A composition comprising at least one selected from elastin, hyaluronic acid and chondroitin sulfate, comprising collagen and / or collagen peptide as a main component, and inhibiting AGEs production according to (1) or (2) A composition for improving ED, comprising adding an agent.
(4) A composition comprising at least one selected from elastin, hyaluronic acid, and chondroitin sulfate, comprising collagen and / or collagen peptide as a main component, and inhibiting AGEs production according to (1) or (2) A composition for hair and eyelashes comprising an agent added.
(5) The composition for improving ED or for hair and eyelashes according to (3) and (4), wherein the collagen peptide is an oligopeptide.
(6) The composition for improving ED or for hair and eyelashes according to (3) to (5), wherein the application is in the form of food and drink.
(7) The composition for hair and eyelashes according to (4) or (5), wherein the application is a parenteral external form.
(8) In a composition comprising collagen and / or collagen peptide as a main component and at least one selected from elastin, hyaluronic acid and chondroitin sulfate, an AGEs production inhibitor is added, and the composition is applied To improve the ED.
(9) The AGEs production inhibitor is at least one selected from Ashwagandha, Ikariso, Panax ginseng, guarana, black turmeric, tribulus, tongkat ali, garlic, Pueraria, maca, muirapuama, moringa, mesimacob, and royal jelly. The method for improving ED according to (8), wherein millet is added as an activator for suppressing AGEs production.
(10) A composition comprising at least one selected from elastin, hyaluronic acid and chondroitin sulfate as a main component of collagen and / or collagen peptide, to which an AGEs production inhibitor is added, and the composition is applied To improve scalp function and prevent hair loss and gray hair.
(11) The AGEs production inhibitor is at least one selected from Ashwagandha, Ikariso, Panax ginseng, guarana, black turmeric, tribulus, tongkat ali, garlic, Pueraria, maca, muirapuama, moringa, mesimacob, and royal jelly. The method for preventing hair loss and gray hair by improving the scalp according to (10), wherein millet is added as an activator for suppressing AGEs production.
(12) The method for improving ED or the method for preventing hair loss and white hair by improving the scalp according to (8) to (11), wherein the collagen peptide is an oligopeptide.
(13) The method for improving ED according to (8) and (9), wherein the application is a food and drink form.
(14) The method for preventing hair loss and gray hair by improving the scalp according to (10) and (11), wherein the application is a parenteral external form.

According to the present invention, by applying a specific AGEs production inhibitor optimally in an external and / or oral form, a decrease in the amount of collagen and elastin in the skin tissue can be prevented, and functional improvement and normalization can be achieved. As a result, it is possible to keep the skin moisturized and the skin and penis firmness and luster in the diet, and to normalize the female ovulation function and male sex function, particularly ED. Furthermore, the ED improvement can also have a ripple effect as a treatment for male infertility.
In the scalp, the function of the melanocytes adjacent to the hair matrix cells is normalized, and the hair activity (improves firmness and gloss) as well as the prevention of hair loss and white hair. The eyelid skin can retain the elasticity and elasticity of eyelashes, and can prevent damage caused by so-called extensions. Furthermore, according to the present invention, these effects can be enhanced by the addition of an activator of AGEs production suppression, particularly millet. Since millet itself has a hair growth effect, when used as a material for improving scalp function, not only the activation effect of inhibiting AGEs production but also the dual effect as an active ingredient for hair growth can be exhibited.

  Hereinafter, specific modes for carrying out the present invention will be described in detail. However, the technical scope of the present invention is not limited to the specific modes disclosed below.

  The first invention in the present invention is a specific AGEs production inhibitor found not only for preventing skin aging due to aging and for improving rough skin during dieting but also for improving hair and the like and for improving ED. In other words, blood flow, nourishment in view of the use of female infertility treatment, eyelashes, scalp care (hair function improvement / hair loss and white hair prevention) and ED improvement (use including male infertility treatment related to the use) After screening for materials that have an AGEs-inhibiting effect from materials with a tonic effect, Ashwagandha, Ikarisou, Panax ginseng, Guarana, Black turmeric, Tribulus, Tonkat Ali, Garlic, Pueraria, Maca, Muirapuama, Moringa, Meshimakob and Royal Jelly Was found as a suitable material.

Here, Ashwagandha is the scientific name “Withania somnifer” and is an evergreen shrub of the solanaceous family that grows naturally in India. In India, it has long been used as Ayurveda.

Epimedium grandiflorum var. Thunbergianum is a deciduous perennial plant belonging to the genus Eucalyptus, and the whole plant is famous as a herbal medicine called oleam.

Panax ginseng C. A. Meyer is a perennial plant belonging to the family Araceae, whose main medicinal site is the root and the useful ingredient is a saponin group called ginsenoside, which has been taken for a long time.

Guarana (Paullinia cupana) is a vine plant belonging to the genus Guarana, which is from the Amazon River basin. Actually, it is small and round, and grows like a tuft. The skin is red, and when ripe, the fruit flips and black seeds appear. Guarana seeds are rich in caffeine and tannins.

Black turmeric (Kaempferia parviflora), also known as “Kurachaidum” or “Black Ginger”, is a plant belonging to the genus Kempelia in the tropical region. In Japan, it is cultivated only in Okinawa.

Tribulus Terrestris is a terrestrial perennial that grows naturally from South Asia to Eastern Europe.

Tonkat Ali is a species of oyster family shrub found in tropical rainforests such as Malaysia, Indochina and Sumatra, and reaches 10 meters in height when grown.

Garlic (Allium sativum) is a perennial plant belonging to the genus Allium and uses bulbs as bulbs.

Pueraria mirifica (Scientific name: Pueraria mirifica) is a plant belonging to the genus Leguminosae that grows naturally in deciduous forests in subtropical regions such as the mountainous areas of Myanmar.

Maca (genus name Lepidium) is a cruciferous perennial plant that grows in Peru, South America.

Muirapuama (Ptychopetalum olacoides) is a kind of herb that lives in the Amazon.

Moringa (scientific name: Moringa oleifera Lam.) Is a Japanese name, also called wasabi tree, and grows naturally in the subtropical and tropical regions. This is particularly common in North India and the Philippines.

Meshimakobu (Phellinus linteus) is a medicinal mushroom native to Japan.
“Meshima” in the gender archipelago of Nagasaki Prefecture is named “mump” -shaped mushrooms that parasitize the trunk of wild “mulberry”. It belongs to the genus Mushroom.

  Royal jelly is a substance that bees eat pollen and honey, break down, synthesize, and secrete.

These AGEs production inhibitors according to the present invention may be used as raw materials as powders or the like, but it is preferable to use an extract extracted with an aqueous solvent for each material in order to exhibit its activity efficiently. . As the aqueous solvent, water and / or an extract extracted with a lower alcohol can be used, and ethanol is preferable as the lower alcohol.

In this invention, millet can be used in order to enhance the AGEs production inhibitory effect to 1 or more types chosen from these specific AGEs production inhibitors.

Here, millet means millet and is a general term for cereals that are not main grains. Minor grains refer to cereals included in the herbaceous family of millet in the narrow sense, but in the broader sense, cereals other than millet include sorghum and pearl barley in the genus Sorghum, and beans of soybeans and red beans. Etc. In the present invention, among these, especially the annual grasses of the family Gramineae (Panicum miliaceum) and pearl millet (Pennisetum glaucum). The pearl millet is a monocotyledonous herb belonging to the genus Chileashiba, and its English name is called Pearl millet. There is another name for black millet.

The millet of the present invention may use the raw material itself by making seeds into powder etc., as with the AGEs production inhibitor, but it is preferable to use an extract extracted with an aqueous solvent in order to efficiently exhibit its activity. . As the aqueous solvent, water and / or an extract extracted with a lower alcohol can be used, and ethanol is preferable as the lower alcohol.

Millet extract is known as a hair restorer that has been drunk from the Middle Ages of Europe, but its use as an activator of AGEs production inhibitor as in the present invention is not known.

In the present invention, one or more selected from the above-mentioned specific AGEs production inhibitors or a composition obtained by adding millet to these can be used as they are, but an active ingredient (functional ingredient) for application as an external preparation or a form of food and drink ) Can also be used.

When applied externally, the combination use of a known percutaneous absorption enhancer is optional, and the formulation is designed according to the site of use. By applying these, the glycation reaction of proteins such as collagen in the epidermis and dermis can be suppressed, and the effects useful for improving skin firmness and wrinkles and preventing rough skin during dieting can be exhibited. Moreover, by applying to the scalp, production and accumulation of AGEs in the scalp can be suppressed, and hair matrix cells are activated. Moreover, the function of melanocytes is normalized. As a result, hair loss can be prevented and white hair can also be prevented. Furthermore, in the penis, the absorbed / aged AGEs production inhibitor can suppress the adhesion / accumulation of AGEs to the blood vessel wall, so that the oxidation of nitric oxide by active oxygen can be suppressed and the blood flow can be improved. In addition, the nutritional tonic and blood flow promoting function inherent in the material of the present invention are combined, and blood flow improvement in the penile cavernous body is further promoted and erection power is improved. In addition, since the tissue of the penis part has good penetrability and absorbability of drugs and the like, a sufficient effect can be expected even by applying an external preparation, but expectation is still expected when combined with oral use such as food and drink. Especially when intended for the treatment of infertility in women, the effective concentration in the blood can be maintained by delivering an anti-glycation agent into the body by eating and drinking, so oral intake is optimal because it can strongly suppress egg degradation due to saccharification Is the method.

The external preparation of the present invention includes forms acceptable as pharmaceuticals, quasi drugs, and cosmetics, and the dosage form is not particularly limited as long as it does not impair the object of the present invention. Examples thereof include known forms such as creams, ointments, emulsions, liniments, pastes, lotions, emulsions, essences, gels, shampoos, rinses, conditioners and tonics.

  As the formulation base (excipient) at the time of formulation design, any liquid and solid raw materials acceptable for skin application can be used widely. At that time, various general-purpose additives such as a humectant, a preservative, and an antioxidant can be added as necessary.

Examples of humectants include urea, glycerin, propylene glycol, 1,3-butylene glycol, sorbitol, mannitol, polyethylene glycol, dipropylene glycol and other polyhydric alcohols, ceramides, heparin-like substances, glucosamine, fucoidan, Examples include trimethylglycine, betaine, seaweed extract (Arge colloid) and the like. The combined use of the moisturizing agent is a useful choice as a prescription design particularly when intended to improve rough skin that may occur during dieting.

  Examples of the preservative include benzoate, sorbate, dehydroacetate, p-hydroxybenzoate, benzalkonium chloride, hinokitiol, resorcin, ethanol, phenoxyethanol, and the like.

  Examples of the antioxidant include dibutylhydroxytoluene, butylhydroxyanisole, propyl gallate, ascorbic acid and the like.

  In addition to the active ingredient of the present invention, other functional ingredients usually used in external preparations can be arbitrarily blended within a range not impairing the effects and purposes of the present invention. Specifically, for example, lysozyme chloride, glycyrrhizic acid or salts thereof, anti-inflammatory agents such as allantoin, immunostimulants such as beta-glucan, male hormone (methyltestosterone), glucosyl hesperidin, citrulline, vitamin E nicotinate, nicotinic acid, nicotine Peripheral vasodilators such as acid amide, benzyl nicotinate, ginger tincture, assembly extract, pepper tincture, antibacterial agents such as hinokitiol, benzalkonium chloride, undecylenic acid, placenta extract, liver extract, oyster extract, Artea Extract, Ononis extract, Kawaramugi extract, Kidachi aloe extract, Red ginseng extract, Deer horned turf extract, Persimmon (banana peel) extract, Ginseng extract, Japanese persimmon root extract, Sasayuri extract, Western yarrow extract Liquid, western nanban (dry seeds) ) Extract, Sage extract, Celery extract, Soybean extract, Tachiko extract, Chimpi extract, Hikikoshi extract, Fukidanpopo extract, Button extract, Macombe extract, Melissa extract, Yuzu extract, Resvera Examples include various extracts derived from animals, plants and microorganisms such as trawl and lactic acid bacteria culture extract, and extracts obtained by fermenting vegetables, fruits and wild grasses (hereinafter referred to as wild grass fermented extracts). Examples of the raw material for fermenting wild grass include, for example, mugwort, dokudami, kitachialoe, turmeric, ginseng, wolfberry, pigeon, hypericum, umbilical cabbage, litchi, dandelion root, gypsophila, peach leaf, ginkgo biloba, edis gusano Seeds, Ninja, Fig leaf, Hubgrass, Safflower, Ezokogi, Achacharu, Lacanka, Eucommia leaf, Enmay grass, Loquat leaf, Morohaya, wolfberry leaf, Settsutsuboku, Akamegashiwa, Psyllium, Tulsi, Daylily, Pine leaf, Nanten leaf, Japanese cedar, Amadokoroko, Tsuyukusa, Megusuri tree, Tsuruna, Yam, Oyster leaf, Chamomile, Karin, Perilla leaf, Tangerine peel, Ginger, Mulberry leaf, Senshinren, Kumquat, Onion husk, Seven ginseng, Kyodo root, Jujube , Salacia, pepper, yew, red ginseng, Louis , Amla berries, boxweed, boxed bean, muirabuama, ibonashi tsuji rakuji, cat's claw, kombu, funori, mandarin, apple, pineapple, grape, fig, ume, melon, grapefruit, lemon, apricot, carrot, onion, parsley, burdock , Garlic, rakkyo, cabbage, shiitake and the like can be appropriately selected and used according to the application.

  Further, examples of other AGEs production inhibitors include plant extracts such as flax, amadokoroko, olives, kanaoi, lindens, citrus radish, elderberry, roses, sunflowers, purple flowers, cypresses, and walnuts.

As oral forms, food and drink, etc., for example, it can be formulated into known forms such as capsules, tablets, granules, powders, syrups, liquids and the like and used freely. Specific examples of foods and drinks include, for example, nutritional supplements such as drinks and supplements, foods for specified health use, functional foods, health foods, and various general processing of soft drinks, soups, cookies, candy, gummies, gums, etc. Food. As the excipient at that time, any liquid and solid raw materials that are acceptable for internal use can be widely used. However, in consideration of the effect of anti-glycation, caution is required in selecting a sweetener. That is, those that are difficult to saccharify should be selected. For example, sugar alcohols such as sorbitol, maltitol, erythritol, lactitol, xylitol, trehalose and stevia, rahan fruit extract, aspartame of synthetic sweetener, and the like are preferably used.

  As a parenteral supplement such as food and drink, a known nutritional tonic or blood circulation promoter may be used in combination as long as the effects of the present invention are not impaired.

For example, as a nourishing tonic, there can be exemplified a suppon extract, a beetle extract, a cinnamon tincture, a loachfish extract, an Ezocogi extract, a Tochu extract, a kashu extract and the like.

Examples of the blood circulation promoter include glucosyl hesperidin, citrulline, vitamin E nicotinate, nicotinic acid, nicotinic acid amide, benzyl nicotinate, ginger tincture, chili pepper tincture, and rosemary acid.

The second invention is an invention that can be made in the process of pursuing the first invention described above, and provides an optimal composition for improving ED and improving hair / lash function.

That is, according to the present invention, a specific AGEs production inhibitor is added to a composition comprising at least one selected from elastin, hyaluronic acid and chondroitin sulfate based on collagen and / or collagen peptide. Thus, an ED improving composition is provided.

Further, according to the present invention, a specific AGEs production inhibitor is added to a composition comprising at least one selected from elastin, hyaluronic acid, and chondroitin sulfate as a main component of collagen and / or collagen peptide. Thus, a composition for hair and eyelashes is provided.

Here, the specific AGEs production inhibitor added to the composition for improving ED and the composition for hair and eyelashes is Ashwagandha, Ikarisou, Panax ginseng, guarana, black turmeric, tribulus, tonkat ant, garlic, Pueraria, It is at least one selected from maca, muirapuama, moringa, mesimacob, and royal jelly, and further contains millet as an activator for AGEs production suppression effect.

  Collagen is not particularly restricted, but considering absorbability, collagen peptides, particularly oligopeptides composed of “proline-hydroxyproline” and / or “hydroxyproline-glycine” (less than 10 peptides) and atelocollagens preferable.

  Elastin is an elastic fiber having a role to support collagen fibers isolated from mammals such as pigs and cattle and fish such as tuna and salmon. In consideration of absorbability in the body, it is preferable to use those having an average molecular weight of around 100,000, which are enzymatically hydrolyzed by proteolytic enzymes such as elastase, pepsin and pronase, or hydrolyzed by acid. Furthermore, those having an average molecular weight of about 20,000 are preferable because of easy solubilization.

  Hyaluronic acid is a high-molecular polysaccharide in which N-acetylglucosamine and glucuronic acid are bonded to each other in beta 1,4, and preferably has a low molecular weight obtained by degrading hyaluronic acid with an enzyme such as hyaluronidase (average molecular weight around 100,000). Can be used.

  Chondroitin sulfate is a simple substance such as chondroitin 6-sulfate and chondroitin 4-sulfate, or a mixture thereof. The molecular weight is not particularly limited, but an average molecular weight of 50,000 is preferable in view of absorbability in the body. In the present invention, a chondroitin sulfate salt can also be used, and the type of the salt is not particularly limited, but a sodium salt or potassium salt is preferred.

  The significance of combining collagen and elastin with an AGEs production inhibitor is the dual effect of maintaining the original functionality of the protein in the body while supplementing the absolute amount while suppressing glycation of the protein present in the body with the AGEs production inhibitor. Is what you expect. As a result, the maintenance of homeostasis (homeostasis) is achieved.

The application of the active ingredient in the second invention, that is, the specific AGEs production inhibitor is the same as in the first invention. The parenteral or oral form is the same as that of the first invention. Collagen, elastin, hyaluronic acid and chondroitin sulfate can be optionally mixed depending on the application site and the form of preparation, but usually the whole preparation 0.01 to 20% by weight, preferably 0.3 to 10% by weight is sufficient.
The AGEs production inhibitor is preferably in the range of 1: 0.01 to 1:20, preferably 1: 0.1 to 1:10, based on the blended amount.

As 3rd invention, the usage method of the composition which concerns on 2nd invention is provided.

That is, according to the present invention, in a composition comprising at least one selected from elastin, hyaluronic acid and chondroitin sulfate based on collagen and / or collagen peptide as a main component, an AGEs production inhibitor is added, A method of improving ED or also improving scalp function and preventing hair loss and gray hair is provided.

Further, according to the present invention, in a composition comprising at least one selected from elastin, hyaluronic acid and chondroitin sulfate as a main component of collagen and / or collagen peptide, an AGEs production inhibitor is added, Furthermore, there is provided a method for improving ED characterized by adding millet as an AGEs production inhibitory effect activator or a method for improving scalp function and preventing hair loss and gray hair.

  A composition containing collagens as a main component is combined with a specific AGEs production inhibitor, and the glycation reaction is suppressed while adjusting the absolute amount of collagen and the like in vivo, thereby improving ED and preventing hair loss and white hair. The idea of using the composition of the present invention is a novel invention that has never been seen before.

  In the third invention, the AGEs production inhibitor or composition thereof obtained in the first and second inventions is taken daily in an oral form such as food and drink, preferably between meals, and for external use, Appropriate amount at least once a day should be applied to the affected area. Ideally, take it daily orally and continue internal care, and take external care when using. That is, it is preferable in terms of effect that oral care is the main and external care is the secondary.

  As described above, according to the present invention, AGEs production suppression that can solve the problems of in vivo saccharification reaction, in particular, the absolute amount of in vivo proteins such as collagen and elastin lost due to aging and functional decline. An agent or a composition thereof and a method of using these agents are provided, thereby improving ED and preventing hair loss and white hair.

Hereinafter, the present invention will be described more specifically by way of examples (manufacturing examples and test examples and formulation examples of effects), but the scope of the present invention is not limited to these examples.
<Production Example 1>
5 kg of water was added to 100 g of dried Ashwagandha leaves, extracted at room temperature for 24 hours and filtered to obtain an Ashwagandha extract.
<Production Example 2>
Water (5 kg) was added to 500 g of dried icarius plant, heated and extracted at 40 ° C. for 3 hours, and filtered to obtain a licorice extract.
<Production Example 3>
200 g of dried ginseng root was decocted with 5 kg of water and filtered to obtain a ginseng extract.
<Production Example 4>
500 g of guarana seeds were crushed, 1 kg of water and 4 kg of ethanol were added, extracted by heating at 40 ° C. for 5 hours, and filtered to obtain a guarana extract.
<Production Example 5>
Chop 200 g of black turmeric, add 5 kg of water, and roast. Thereafter, filtration was performed to obtain a black turmeric extract.
<Production Example 6>
3 kg of water and 2 kg of ethanol were added to 300 g of Tribulus leaves, extracted at room temperature for 5 hours, and filtered to obtain a Tribulus extract.
<Production Example 7>
Tonkat ant root is powdered and 200 g is decocted with 5 kg of water. Filtration was performed to obtain a tonkat ant extract.
<Production Example 8>
After slicing 50 g of garlic and adding 5 kg of water, it was extracted at room temperature for 24 hours and filtered to obtain a garlic extract.
<Production Example 9>
100 g of Pueraria was chopped, 1 kg of water and 4 kg of alcohol were added, extracted at 40 ° C. for 6 hours, and filtered to obtain a Pueraria extract.
<Production Example 10>
100 g of dried maca root was powdered, extracted with 5 kg of hot water, and then filtered to obtain a maca extract.
<Production Example 11>
300 g of muirapuama bark was made into powder, 3 kg of water and 2 kg of ethanol were added and extracted at 60 ° C. for 5 hours, followed by filtration to obtain a Murapuama extract.
<Production Example 12>
Add 5 kg of water to 500 g of Moringa leaves and decoct for 6 hours. After filtration, a Moringa extract was obtained.
<Production Example 13>
300 g of fruit body of Meshimakobu was chopped, 500 g of water and 4.5 kg of ethanol were added, extracted at 40 degrees for 24 hours, and filtered to obtain Meshimakobu extract.
<Production Example 14>
5 kg of ethanol was added to 500 g of raw royal jelly, extracted at room temperature for 10 hours, and then filtered to obtain a royal jelly extract.
<Production Example 15>
100 g of dried millet was powdered, 5 kg of water was added, extracted at 50 degrees for 24 hours, and then filtered to obtain a millet extract.

<Test Example 1> AGEs production inhibitor test (test method)
The entire test was performed according to the method of Yokozawa et al. (Yokozawa, T .; Nakagawa, T .; Journal of Traditional Medicines. 18, 107-112 (2001)).
(1) Preparation of reaction solution D-glucose, D-(-)-fructose and BSA (bovine serum albumin) were added to 100 mM phosphate buffer (pH 7.4) and dissolved, and 25 mM, 25 mM, 10 mg / kg, respectively. The reaction solution was prepared to be mL.
(2) Preparation of sample The sample (each extract of Production Examples 1 to 15) and the control used purified water for the test. In addition, aminoguanidine was used as a comparative control (positive control) and prepared with purified water to a final concentration of 0.2 mM.
(3) Test procedure The sample and control prepared to each concentration were added to each test tube, a phosphate buffer solution was added to the control, and a reaction solution was added to other than the control, followed by airtight mixing. These test solutions were incubated at 60 ° C. for 18 hours. After the incubation, the test tube was cooled on ice and returned to room temperature, and the fluorescence intensity was measured with a spectrofluorometer (wavelength; excitation 360 nm, fluorescence 450 nm). The AGEs production inhibition rate was determined by the following equation.

AGE production inhibition rate (%) =
[1- (Sample fluorescence intensity−Sample blank fluorescence intensity / Control fluorescence intensity
-Fluorescence intensity of control blank)] × 100

(Results and discussion)
The results are shown in Table 1. As is clear from this result, it is clear that all of the extracts of the present invention have a high AGEs production inhibitory effect, and by applying these optimally in an external and / or oral form, Reduction of the amount of collagen and elastin can be prevented, and the functional improvement and normalization targeted by the present invention can be achieved.

<Test Example 2> Use test (test method)
A use test was conducted by a male panelist at the age of 50.
The evaluation panel was divided into a group to which the invention product (drink preparation containing the active ingredient) was applied and a placebo (the other base other than the active ingredient was the same drink preparation). And about each paneler, the improvement effect of this invention composition compared with before use after 6 months use was confirmed with the following evaluation items.

Evaluation 1 Hair improvement effect 1 Degree of hair firmness, firmness and hair loss Great improvement Improvement Slightly improved 4. Invariant 5. Deterioration evaluation 2 Hair improvement effect 2 Prevention of occurrence of white hair in hair 1. Great improvement Improvement Slightly improved Invariant 5. Deterioration evaluation 3 Eyelash improvement effect The degree of elasticity, firmness and gloss of eyelashes Great improvement Improvement Slightly improved Invariant 5. Deterioration assessment 4 Male function improvement effect Erection / sexual function (sustainability)
1. Great improvement Improvement Slightly improved Invariant 5. Deterioration (test sample)
1. Active ingredient a group 100 mL of purified water is added to 15,000 mg of collagen peptide, 300 mg of elastin, 130 mg of sodium hyaluronate and 130 mg of chondroitin sulfate, and mixed with stirring.
2. As an active ingredient b group AGEs production inhibitor, 100 mL of purified water is added to 100 mg of the extract (dried product) of Production Examples 1 to 15 and stirred and mixed.
3. Sample preparation 1 was added to 2 and mixed by stirring. After preparing a proper amount of trehalose and stevia as sweeteners, a small amount of flavoring was added to the sample and used as a sample.

(Results and discussion)
The results are shown in Table 2. As is apparent from this result, the composition of the present invention was found to have excellent hair improving effect, eyelash improving effect and male sexual function improving effect. In view of the effect of improving the male sexual function, the composition of the present invention can be sufficiently expected to be effective in treating male infertility.

Although the formulation example of this invention is given to the following, the scope of the present invention is not limited to the following formulation example.

Table 3 shows examples of drink formulations. As with the results shown in Test Example 2, all of these five formulations had the intended effect of the present invention.

Table 4 shows formulation examples of lotions. As with the results shown in Test Example 2, all of these five formulations had the intended effect of the present invention.

Table 5 shows examples of hair restorer formulations. As with the results shown in Test Example 2, all of these five formulations had the intended effect of the present invention.

Table 6 shows examples of shampoo formulations. As with the results shown in Test Example 2, all of these five formulations had the effect on hair application that is the object of the present invention.

Table 7 shows an example of the conditioner formulation. As with the results shown in Test Example 2, all of these five formulations had the effect on hair application that is the object of the present invention.

Claims (14)

An AGEs production inhibitor comprising one or more selected from Ashwaganda, Epimedium, Panax ginseng, Garana, Black Turmeric, Tribulus, Tonkat Ali, Garlic, Pueraria, Maca, Muirapuama, Moringa, Meshimakobu and Royal Jelly. The AGEs production inhibitor according to claim 1, wherein millet is added as an activator for AGEs production inhibitory effect. Addition of the AGEs production inhibitor according to claim 1 or 2 to a composition comprising at least one selected from elastin, hyaluronic acid and chondroitin sulfate, comprising collagen and / or collagen peptide as a main component A composition for improving ED, characterized by comprising: Addition of the AGEs production inhibitor according to claim 1 or 2 to a composition comprising at least one selected from elastin, hyaluronic acid and chondroitin sulfate, comprising collagen and / or collagen peptide as a main component The composition for hair and eyelashes characterized by these. The composition for improving ED or hair / lashes according to claim 3 or 4, wherein the collagen peptide is an oligopeptide. 6. The composition for improving ED or for hair and eyelashes according to claim 3, wherein the application is in the form of food or drink. The composition for hair and eyelashes according to claim 4 or 5, wherein application is a parenteral external form. In a composition comprising collagen and / or collagen peptide as a main component and at least one selected from elastin, hyaluronic acid and chondroitin sulfate, an AGEs production inhibitor is added, and ED is obtained by applying the composition. How to improve. The AGEs production inhibitor is at least one selected from Ashwagandha, Ikarisou, Panax ginseng, Garana, Black turmeric, Tribulus, Tongkat Ali, Garlic, Pueraria, Maca, Muirapuama, Moringa, Meshimakob, and Royal Jelly, and further inhibits AGEs production 9. The method for improving ED according to claim 8, wherein millet is added as an effect activator. In a composition comprising collagen and / or collagen peptide as a main component, and comprising at least one selected from elastin, hyaluronic acid and chondroitin sulfate, by adding an AGEs production inhibitor and applying the composition How to improve scalp function and prevent hair loss and gray hair. The AGEs production inhibitor is at least one selected from Ashwagandha, Ikarisou, Panax ginseng, Garana, Black turmeric, Tribulus, Tongkat Ali, Garlic, Pueraria, Maca, Muirapuama, Moringa, Meshimakob, and Royal Jelly, and further inhibits AGEs production The method for preventing hair loss and gray hair by improving the scalp according to claim 10, wherein millet is added as an effect activator. 12. The method for improving ED or the method for preventing hair loss and white hair by improving the scalp according to claim 8, wherein the collagen peptide is an oligopeptide. 10. The method for improving ED according to claim 8 or 9, wherein the application is a food and drink form. 12. The method for preventing hair loss and white hair by improving the scalp according to claim 10, wherein the application is a parenteral external form.