KR102509801B1 - Functional composition for hair loss relief and hair growth containing sturgeon water-soluble oil - Google Patents

Abstract

The present invention relates to a functional composition for hair loss relief and hair growth, and more particularly, to a functional composition for hair loss relief and hair growth, which contains sturgeon water-soluble oil as an active ingredient, and while exhibiting excellent efficacy in the hair loss relief and hair growth, there is no concern about side effects such as allergies by including ingredients derived from natural ingredients, thereby being more safely and easily taken and applied to the skin, and to a method for producing the same.

Description

Functional composition for hair loss relief and hair growth containing sturgeon water-soluble oil}

The present invention relates to a functional composition for hair loss relief and hair growth, and more specifically, to a functional composition containing sturgeon water-soluble oil as an active ingredient and exhibiting excellent efficacy in hair loss relief and hair growth, including ingredients derived from natural materials, to prevent allergies, etc. It relates to a functional composition for hair loss relief and hair growth, which can be more safely and easily applied to the diet and skin, and a method for producing the same, because there is no concern about the side effects of the present invention.

Sturgeon ( Acipenser sinensis ) is a fish species originating from 450 to 350 million years ago (Devonian Period), belonging to the Acipenseridae family, and is a fish species that is widely distributed in the northern mid-latitude region of the Northern Hemisphere and grows naturally. Currently, 26 species of sturgeon are known, and interest in many countries is increasing due to their economic and ecological value.

In March 1997, Siberian sturgeon, which was first transplanted and bred in Korea at Canakov Farm in Russia, and then produced in 2003 by its own seedlings, has excellent environmental adaptability, grows faster than other breeds farmed in Korea, and has good meat quality. It has become a popular fish for aquaculture. It can reach a maximum length of 3 m, and can grow to a weight of 200 to 300 kg, with some species reaching a maximum length of more than 8 m and a maximum lifespan of more than 100 years. At about 6 to 7 years old, they grow to more than 1 m and produce caviar.

Sturgeon has a chewy taste of flesh, so it is used in raw, smoked, barbecued, steak and skewered dishes, its head is steamed and stewed, its fin is used in shark fin dish, which is one of the three major delicacies in China, and its cartilage is used for special dishes, tonics and industrial ingredients. there is. In addition, sturgeon contains a large amount of active ingredients such as protein, essential amino acids, unsaturated fatty acids, vitamins, EPA, DHA, etc. As the excellent efficacy of is revealed, it is getting more and more attention as a highly nutritious and highly functional material.

In recent years, as sturgeon farming technology has begun to develop, there are more opportunities for common people to come into contact with sturgeon. It became.

However, in the raw material of sturgeon marketed to date, sufficient research has not been conducted on the actual content of sturgeon, the content of active ingredients, processing method, extraction efficiency, etc. In particular, since there is no processing technology for sturgeon and products using it in Korea, sturgeon has not yet been objectively and publicly recognized by consumers as a functional material and product.

Furthermore, not only the processing technology of sturgeon, but also the various functionalities and useful physiological activities of active ingredients obtained from sturgeon have not been sufficiently studied to date, so the future development possibilities for this field are limitless.

On the other hand, alopecia is one of the relatively common diseases that begin in adults after puberty, and as the standard of living improves and social interest in skin beauty increases, interest in treatment and prevention of the disease gradually increases.

Genetic factors, wrong diet, stress, seborrheic dandruff, endocrine abnormalities, etc. are known to cause hair loss, and external use products used for the purpose of relieving hair loss symptoms are functional cosmetics, and the Ministry of Food and Drug Safety examines safety and efficacy for each item and approves them. .

Androgenetic alopecia caused by male hormones and heredity occurs in about 50% of both men and women, and it is common to start in the 40s and 50s, but in severe cases, it starts right after puberty and lasts for decades. In both men and women, hair loss mainly starts at the top of the head and gradually progresses to the entire head. In the case of men, regression of the hairline on both sides of the temporal region and hair loss predominate in the top of the head, and in the case of women, the borderline of the facial scalp hair It is generally well preserved, and it is common to have a Christmas tree shape, and complete bald areas are rare.

Products that reduce male hormones, which are known to cause hair loss, have been developed and have been expected to have a hair loss treatment effect. In addition, in the case of drug therapy, the effect on the scalp area where hair growth is most needed in appearance has not reached the expected level.

Accordingly, as a result of intensive research on the sturgeon-derived complex developed by the present applicant, it was found that it has an effect of relieving hair loss and promoting hair growth, and furthermore, a formulation that can be safely and easily applied to the human body has been developed. The present invention has been completed.

Republic of Korea Patent Publication No. 10-2018-0133101

The present invention is intended to solve the above problems, and uses sturgeon water-soluble oil as an active ingredient and exhibits excellent efficacy in mitigating hair loss and hair growth, while containing ingredients derived from natural materials, so there is no concern about side effects such as allergies. Therefore, it is intended to provide a functional composition for hair loss relief and hair growth that can be more safely and easily applied to the diet and skin, and a method for producing the same.

The above and other objects and advantages of the present invention will become apparent from the following description of preferred embodiments.

The above object can be achieved by a functional composition for hair loss relief and hair growth containing sturgeon water-soluble oil obtained from whole sturgeon as an active ingredient.

Based on the total weight of the composition, the sturgeon water-soluble oil is included in 10 to 99.5% by weight, and the sturgeon water-soluble oil promotes the proliferation of dermal papilla (HDP) cells, and the composition comprises , It is characterized in that it is prepared as a composition for applying to the scalp or a liquid composition for eating.

The sturgeon water-soluble oil is prepared by preparing sturgeon oil according to a raw material obtaining method of whole sturgeon; Performing a first aging of the sturgeon oil to prepare an aged sturgeon oil; A pre-treatment step of foaming the aged sturgeon oil by using a high-pressure processor and a fine nozzle to form changes in physical properties of the oil; Second aging of the pretreated sturgeon oil aging product;

Mixing the aged sturgeon oil and water, in which the second aging has been performed, and introducing the mixture into a distillation extractor and performing a second extraction; After performing the second extraction, water is mixed with the remaining matured sturgeon oil and introduced into a distillation extractor, and third extraction is performed to produce first water-soluble oil; Preparing a first additive composition containing edible snails and a second additive composition containing Puer tea and chrysanthemum, respectively; and preparing a secondary water-soluble oil by distilling and extracting a mixture of the primary water-soluble oil, the primary additive composition, and the secondary additive composition.

According to the present invention, it is possible to provide a functional composition for hair loss relief and hair growth containing sturgeon water-soluble oil.

The functional composition may be prepared as a dietary composition containing ingredients derived from natural materials so that it can be used more safely and easily without side effects such as allergies, and a skin application composition including the scalp. Accordingly, the functional composition can be utilized in various industrial fields, such as, for example, food, drinking water, cosmetics, pharmaceuticals, pet-related products, and vegetable raw material compositions.

The sturgeon water-soluble oil can secure human body stability and environmental friendliness by excluding artificial chemicals such as surfactants and forming a water-soluble oil formulation only by physical separation using natural nanotechnology.

In addition, the sturgeon water-soluble oil composition contains sturgeon oil obtained from whole sturgeon, contains a high content of active ingredients in sturgeon, and can secure human safety and improved quality due to non-antibiotics .

However, the effects of the present invention are not limited to the effects mentioned above, and other effects not mentioned will be clearly understood by those skilled in the art from the description below.

1 is photographs of the preparation of compositions of Examples 1 and 2 and Comparative Examples 1 and 2 according to an embodiment of the present invention.
Figure 2 is a photograph of the preparation of the composition of Example 3 and Comparative Example 3 according to an embodiment of the present invention.
3 is a graph showing the results of Experimental Example 1, which is a cell test.
4 is a photograph confirming the hair growth effect for each week in Experimental Example 2, which is an animal test.
5 and 6 are photographs and graphs of measuring the number of follicles in Experimental Example 2, which is an animal test.
7 and 8 are photographs and graphs of measuring follicle diameter and dermal thickness in Experimental Example 2, which is an animal test.

Hereinafter, the present invention will be described in detail with reference to embodiments and drawings of the present invention. These examples are only presented as examples to explain the present invention in more detail, and it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples. .

In addition, unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of skill in the art to which this invention belongs, and in case of conflict, this specification including definitions of will take precedence.

In order to clearly explain the proposed invention in the drawings, parts irrelevant to the description are omitted, and similar reference numerals are attached to similar parts throughout the specification. And, when a certain component is said to "include", this means that it may further include other components without excluding other components unless otherwise stated. Also, a “unit” described in the specification means one unit or block that performs a specific function.

In each step, the identification code (first, second, etc.) is used for convenience of description, and the identification code does not describe the order of each step, and each step does not clearly describe a specific order in context. It may be performed differently from the order specified above. That is, each step may be performed in the same order as specified, may be performed substantially simultaneously, or may be performed in the reverse order.

Hereinafter, embodiments and embodiments of the present disclosure will be described in detail with reference to the accompanying drawings. However, it may not be limited to these implementations and examples and drawings herein.

One aspect of the present application provides a functional composition for hair loss relief and hair growth, containing sturgeon water-soluble oil obtained from whole sturgeon as an active ingredient.

Based on the total weight of the composition, the sturgeon water-soluble oil may be included in an amount of 10 to 99.5% by weight, specifically, 30 to 99.5% by weight, more specifically, 50 to 99.5% by weight.

The functional composition for mitigating hair loss and growing hair can exert an effect of alleviating hair loss and growing hair by promoting the proliferation of dermal papilla (HDP) cells to increase the number and thickness of hair follicles.

The hair loss relief and hair growth functional composition may be prepared as a composition having uses such as food, beverage, cosmetic, and pharmaceutical use. Compositions having the above-described uses may be prepared in various formulations to suit each use.

More specifically, the functional composition for hair loss relief and hair growth according to the present invention may be formulated into a composition for application to the scalp or a liquid composition for diet.

The edible liquid composition may be prepared in a formulation selected from the group consisting of, for example, a solution, an aqueous solution, a suspension, and an extract (essence). However, it is not limited thereto, and may be prepared in any formulation commonly prepared in the art.

For example, the edible liquid composition may be prepared as a solution or an extract (liquid) formulation.

The water-soluble sturgeon oil is obtained from whole sturgeon, which is a natural material, and has excellent hair loss relieving and hair growth promoting effects, and human safety can be secured without concern for side effects such as allergies.

Hereinafter, a method for producing the sturgeon water-soluble oil according to an embodiment of the present invention will be described in detail.

Preparation of sturgeon water soluble oil

Preparing sturgeon oil according to the raw material obtaining method in which the sturgeon water-soluble oil is whole sturgeon; Performing a first aging of the sturgeon oil to prepare an aged sturgeon oil; A pre-treatment step of foaming the aged sturgeon oil by using a high-pressure processor and a fine nozzle to form changes in physical properties of the oil; Second aging of the pretreated sturgeon oil aging product;

Mixing the aged sturgeon oil and water, in which the second aging has been performed, and introducing the mixture into a distillation extractor and performing a second extraction; After performing the second extraction, water is mixed with the remaining matured sturgeon oil and introduced into a distillation extractor, and third extraction is performed to produce first water-soluble oil; Preparing a first additive composition containing edible snails and a second additive composition containing Puer tea and chrysanthemum, respectively; and distilling and extracting a mixture of the first water-soluble oil, the first additive composition, and the second additive composition to prepare a second water-soluble oil.

First, sturgeon oil obtained according to the method for obtaining raw materials for sturgeon can be obtained.

The method for obtaining the raw material of the sturgeon includes a farming step (S1) of culturing the sturgeon fry for 3 years or more using natural antibiotics; A pre-processing step (S2) of shipping the farmed sturgeon and pre-processing them whole; A cold storage step (S3) of rapidly cooling the pretreated sturgeon, such that the rapid cooling reaches a temperature of -80 to -20 ° C within 5 to 20 minutes; and thawing and washing the sturgeon stored in the cold, then putting the whole sturgeon into a pneumatic extractor and performing a first extraction to obtain sturgeon oil (S4).

More specifically, in the step S1, sturgeon, specifically, sturgeon fry is farmed. The breeds of the sturgeon include Beluga sturgeon (Huso huso), Kaluga sturgeon (Huso dauricus), Siberian sturgeon (Acipenser baerii), Russian sturgeon (Acipenser gueldenstaedti), stellet sturgeon (Acipenser ruthenus), white It may be selected from the group consisting of sturgeon (Acipenser transmontanus), Amur sturgeon (Acipenser schrenckii), Chinese sturgeon (Acipenser sinensis), spatula sturgeon (Polyodon spathula) and hybrids thereof. Sturgeon according to an embodiment of the present invention may be, for example, a hybrid of Siberian sturgeon and Beluga sturgeon, but is not limited thereto.

The environment of the aquaculture may be a fresh water farm, and the fresh water source may be river water, ground water, mountain spring water, and tap water, and the fresh water source may be a water source that has undergone purification treatment such as dechlorination treatment with activated carbon.

The farming may be carried out until the sturgeon fry become adult sturgeon weighing at least 1 kg, eg for more than 3 years, eg 3 to 5 years.

The aquaculture is oriented toward an antibiotic-free environment. That is, in the sturgeon farming of the present invention, as in the prior art, chemical components such as various antibiotics and growth promoters administered for weight gain and meat quality improvement of the sturgeon are not used, and natural antibiotics made of natural materials are used. can

For example, bay salt may be used as the natural antibiotic. Specifically, by allowing the sturgeon to consume sea salt while being farmed, impurities including harmful bacteria inside the sturgeon can be effectively removed. Intake of the sun salt is preferably carried out within the range of 1 to 3 times a month.

In the above steps S2 and S3, the cultured sturgeon may be pretreated and then stored in a refrigerator. The pretreatment is performed so that the sturgeon is not cut by part, but in the form of whole fish (separated or uncut whole).

The farmed sturgeon is shipped to perform pretreatment, but first, the sturgeon may be fasted so that it does not consume any food from 10 to 12 days before a predetermined shipment date, for example, 10 days before. By allowing the sturgeon to fast, impurities in the intestines are emptied cleanly, so that the yield of active ingredients of the whole sturgeon can be further increased.

The sturgeon after fasting is shipped out, washed briefly, and then knocked down by hitting the sturgeon head once or twice with a rubber mallet. The stunned sturgeon can then be rapidly cooled directly to carry out storage. The rapid cooling condition may be to reach a temperature of -80 to -20 °C in a short time, for example, between 5 and 20 minutes.

By rapidly cooling the sturgeon after rapidly stunning it in the above-described manner, it is possible to obtain high-quality active ingredients of the whole sturgeon while minimizing the pain of the sturgeon.

In the above step S4, sturgeon raw material, that is, sturgeon oil is obtained.

The cooled sturgeon is thawed for 2 to 6 hours, and then the entire fish body is evenly washed with water or saline to remove various impurities such as residual blood or residual intestines.

The first extraction may be performed with the sturgeon in whole form. The first extraction method may be performed using a pneumatic extractor, specifically, a pneumatic hot water extractor. The whole sturgeon may be put into the pneumatic extractor together with water as a solvent. At this time, the solvent may further include calamansi undiluted solution, bellflower undiluted solution and beet undiluted solution.

In one example, an extraction raw material obtained by mixing 50% by weight of sturgeon, 41% by weight of water, 5% by weight of calamansi stock solution, 2% by weight of balloon flower stock solution and 2% by weight of beet stock solution is introduced into the pneumatic extractor to prepare 1 extraction can be performed.

The pneumatic extractor may be performed under conditions of 100 to 104 ° C, for example, 102 ° C, 2.3 to 2.7 atm, for example, 2.5 atm, and the extraction time is 98 to 102 hours, for example, 100 hours it is desirable

After the first extraction, specific gravity separation may be performed in which upper, middle, and lower layers are separated using the difference in specific gravity. As a specific method for the specific gravity separation, first, the obtained primary extract is directly put into the stirrer tank without removing steam (kim) while the pneumatic extractor is operating. In this case, the primary extract contained in the agitator tank may be a liquid extract of sturgeon, and the liquid extract of sturgeon may account for about 80% by weight of the total weight of the raw material for extraction.

Thereafter, a secondary extract may be obtained by performing compression of the remaining extraction raw materials once or twice or more with a compressor provided in the pneumatic extractor. In this case, the secondary extract may be sturgeon oil, and the sturgeon oil may account for about 5% by weight based on the total weight of the raw material for extraction.

Then, the remaining raw material for extraction, that is, solids of sturgeon can be obtained. The sturgeon solids may include sturgeon meat, cartilage, bone marrow, or a combination thereof. The solid content of the sturgeon may account for about 15% by weight based on the total weight of the raw material for extraction.

The method for obtaining the sturgeon raw material described in step S4 above is to perform extraction and gravity separation at the same time, so that all of the sturgeon liquid extract, sturgeon oil and sturgeon solids obtained from whole sturgeon are obtained in a simpler process. make it possible to obtain

Second, a first aging of the sturgeon oil may be performed to prepare an aged sturgeon oil. Sterilization may be performed before performing the first aging. A method of sterilization may be performed according to known techniques generally used in the art.

The first aging is carried out in a shaded place by putting the sturgeon oil in a container for aging, but may be natural aging performed under conditions similar to natural conditions. Specifically, the aging is preferably performed for 25 days to 35 days, for example, 30 days, for example, at a temperature range of 17 to 21 ° C., for example, 17 ° C., without sunlight and without air. It can be performed under the following conditions.

If the first aging is performed within the above-described condition range, impurities in the sturgeon oil may sink or float and be removed later, thereby increasing the purity of the sturgeon oil and further increasing the content of active ingredients. After the aging, filtration may be performed separately.

Thirdly, the aged sturgeon oil may be pretreated. The pretreatment may be an operation of making the oil state immediately before water solubilization (solubilization) in order to obtain a formulation of water soluble oil later.

A specific method of performing the pretreatment is to cause a change in the physical properties of sturgeon oil. For example, the sturgeon oil is passed through a high-pressure injector and a fine nozzle provided in the high-pressure injector several times, tens to hundreds of times, while the sturgeon oil is passed through. It could be foaming shark oil. At this time, the efficiency of the foaming operation may be increased while injecting air using a separate injection pump.

This pretreatment process may be repeated a number of times until the oil becomes a formulation like fresh cream. At this time, the point at which the oil becomes a formulation such as whipped cream may be in a state immediately before water-solubilization (solubilization) to obtain water-soluble oil.

This pretreatment process does not use artificial chemical components such as surfactants used in the past, but only physical nanotechnology causes physical property changes in components to form water solubilization (solubilization) to obtain a formulation of water-soluble oil later. make it possible That is, in one embodiment of the present invention, sturgeon water-soluble oil is prepared using a natural nanotechnology, so that harmlessness to the human body and environmental friendliness should be given priority, drinking water, food, cosmetics, pharmaceuticals, pet-related fields, and vegetable raw material compositions It can be used in a wide range of fields such as

The pre-treated sturgeon oil-aged product may be stored in a container at room temperature, for example, 1 to 35 ° C, specifically, 18 to 23 ° C, after the second aging. The second aging is natural aging like the first aging, and the specific conditions are the same as the first aging, but the aging period is preferably about 10 days.

Fourth, the first water-soluble oil may be prepared by performing second extraction and third extraction with the pretreated aged sturgeon oil and water. At this time, the method of the second extraction and the third extraction may be distillation extraction.

Specifically, in the second extraction, distillation extraction may be performed after adding the pretreated aged sturgeon oil and water in a 1:1 weight ratio to a distillation extractor. Subsequently, after the second extraction, water vapor is all evaporated and water is added to the remaining sturgeon oil in a weight ratio of 1: 1.2 to 1.5, and then third extraction is performed by distillation extraction. The third extraction is repeated at least 30 times, thereby obtaining primary water-soluble oil.

The primary water-soluble oil may be a formulation in the process of preparing a final water-soluble oil. The primary water-soluble oil may be put into a stirrer and stirred once or twice.

Fifthly, an additive composition may be prepared. The additive composition may be a first additive composition containing edible snails and a second additive composition containing Puer tea and chrysanthemum, respectively.

The primary additive composition may further include at least one selected from the group of primary natural ingredients consisting of Baekbong Ogolgae, white throat mushroom, beet, kelp root, and bellflower. For example, the first additive composition may include a first natural ingredient that is a mixture of edible snails, Baekbong Ogolgae, white throat mushrooms, beets, kelp roots, and bellflowers. At this time, the natural ingredient may be used by washing the raw material cleanly and cutting it into an average size of about 4 to 7 cm.

The first natural ingredient extract and water are mixed at a weight ratio of 1:1 and then put into a pneumatic extractor and extracted for 100 hours at a temperature of 100° C. or higher to prepare a first natural ingredient extract, that is, a first additive composition.

The secondary additive composition is a group of secondary natural ingredients consisting of lotus seeds, sea bamboo shoots, centella asiatica, wormwood, ginseng mugwort, peppermint, yugenpi, hagocho, guccipon, waspong, chaga mushroom, samchae, calamansi and mulberry leaves It may further include one or more selected from. In one example, the secondary additive composition is puerh tea, chrysanthemum, lotus seed, haejuksun, centella asiatica, wormwood, ginseng wormwood, peppermint, yugeunpi, hagocho, guccippong, waspong, chaga mushroom, samchae, calamansi and mulberry leaves It may contain secondary natural ingredients that are mixtures. At this time, the natural ingredient may be used by washing the raw material cleanly and cutting it into an average size of about 4 to 7 cm.

After mixing the secondary natural component and water at a weight ratio of 1:1, it is introduced into a pneumatic extractor and extracted for 100 hours at a temperature of 100° C. or higher to prepare a secondary natural component extract, that is, a secondary additive composition.

Subsequently, the primary natural component extract and the secondary natural component extract were aged at room temperature, 1 to 35 ° C, for example, 19 to 23 ° C, for 10 days, and then put together in a stirrer and stirred, Distillation extraction can be performed. For example, 50 parts by weight of the secondary natural ingredient extract and 50 parts by weight of water may be mixed with 100 parts by weight of the first natural ingredient extract, and then put into a distillation extractor for distillation and extraction.

When the distillation extraction is completed, a mixed additive composition obtained from the mixed extraction of the first natural component extract and the second natural component extract can be obtained.

Sixth, a secondary water-soluble oil may be prepared by distilling and extracting the primary water-soluble oil and the mixed additive composition. The primary water-soluble oil and the mixed additive composition may be mixed in a weight ratio of 1:1, and the distillation extraction may be performed once or two to three times.

The secondary water-soluble oil may be "sturgeon water-soluble oil" in the present invention. That is, the water-soluble sturgeon oil may include a mixed additive composition obtained by distilling and extracting a mixture of sturgeon oil and natural additives.

The sturgeon water-soluble oil is an oil formulation that can be easily solubilized in water without artificial chemicals such as surfactants, and in which harmlessness to the human body and environmental friendliness must be given special priority, in the field of drinking water or food, and in the field of cosmetic compositions applied directly to the skin can be utilized for

Preparation of composition for skin application

In one embodiment, a skin application composition containing the previously prepared water-soluble sturgeon oil as an active ingredient may be prepared.

The skin application composition may be prepared in any conventionally prepared formulation that can be directly applied or sprayed to the scalp or hair. For example, it may be formulated as a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, cleansing product, oil, shampoo, conditioner, treatment, aerosol, hair pack, hair wax, spray, etc. It is not limited to this.

The skin application composition may further include commonly used auxiliary components, solvents, and additives according to conditions such as a formulation, preparation, or purpose of use.

For example, in the preparation of a composition for mitigating hair loss and promoting hair growth for skin (scalp) application, for example, alcohol, oil, surfactant, fatty acid, silicone oil, preservative, humectant, humectant, viscosity modifier, emulsion, stabilizer, ultraviolet rays Blocking agents, coloring agents, fragrances, diluents, etc. may be included, and since specific compounds or compositions used therein are already known in the art, those skilled in the art can select and use appropriate compounds or compositions. In addition, the composition may further include a skin absorption accelerator such as niacinamide or panthenol, a scalp protectant, or a scalp activator in order to enhance its effect and minimize side effects.

With respect to the total weight of the composition for mitigating hair loss and promoting hair growth for skin (scalp) application, the water-soluble sturgeon oil is 10 to 99.5% by weight, specifically, 30 to 99.5% by weight, more specifically, 50 to 99.5%, more More specifically, it may be included within the content range of 70 to 99.5%.

In addition, the composition may further include additional ingredients derived from natural ingredients and safe for the human body while increasing the synergistic effects of hair loss alleviation and hair growth promoting effects.

The additional component may further include at least one selected from Cambodian wild plant Conarus semidecandrus extract, soybean violet extract, and combinations thereof. For example, the additional component may be a mixture of a Conarus semidecandrus extract and a bean violet extract, and the mixture may be included in the range of 0.1 to 5% by weight based on the total weight of the composition. Methods for preparing the extracts may be used through design changes by borrowing known technologies.

As the remaining amount, one or more selected from auxiliary components, solvents, and additives according to known techniques may be included in an appropriate content range.

In addition, a specific manufacturing method for preparing or formulating a composition for mitigating hair loss and promoting hair growth for application to the skin (scalp) may follow a known technique commonly used in the art.

Dietary composition preparation

In another embodiment, a dietary composition containing the previously prepared water-soluble sturgeon oil as an active ingredient, specifically, a dietary liquid composition may be prepared.

The dietary composition may be prepared in any formulation that is conventionally prepared, such as a health functional food or health functional beverage prepared for the purpose of relieving hair loss and promoting hair growth so that it can be eaten or eaten. For example, the dietary composition may include functional food, health supplement food, special nutritional supplement food, functional beverage, etc., or the composition according to the present invention may be added to natural food, processed food, etc., but is limited thereto It is not.

The health functional food or health functional beverage in which the dietary composition is formulated may include all foods or beverages in the conventional sense. For example, beverages, various drinks, solutions, aqueous solutions, suspensions, extracts (liquor), tea, functional water, fruits and their processed foods (canned fruit, jam, etc.), fish, meat and their processed foods (ham, bacon, etc.) ), breads and noodles, cookies and snacks, dairy products (butter, cheese, etc.), etc., and may include all functional foods or functional drinks in a conventional sense. In one example, the composition may be prepared as a beverage composition, the form may be produced, for example, liquid stick type, liquid pouch type, etc., but is not limited thereto.

When the dietary composition is formulated into a food or beverage, additives and solvents such as sweeteners, flavors, physiologically active ingredients, mineral preservatives, emulsifiers, acidulants, and thickeners may be further included, if necessary. At this time, it is preferable that the additive is added and used in a very small amount as long as it can achieve the purpose to which it is added. When expressed numerically, a trace amount may range from 0.0005% by weight to about 0.5% by weight based on the total weight.

Based on the total weight of the dietary composition, the sturgeon water-soluble oil is 10 to 99.5% by weight, specifically, 30 to 99.5% by weight, more specifically, 50 to 99.5% by weight, and even more specifically, 70 to 99.5% by weight It may be included within the content range of %.

In addition, the composition promotes the various functionalities of sturgeon water-soluble oil while increasing the synergistic effects of hair loss alleviation and hair growth promoting effects, and may further contain additional ingredients derived from natural ingredients and having human safety.

The additional component may further include at least one selected from wild ginseng extract, rhubarb root extract, soybean violet young leaf extract, roundworm young leaf extract, and combinations thereof. For example, the additional component may be a mixture of wild ginseng extract and rhizome root extract, and the mixture may be included in the range of 0.001 to 3% by weight based on the total weight of the composition.

For example, in the method for preparing the extract of the additional component, the additional component, specifically, 50% of the roots of wild ginseng and red rhubarb, and 50% of sturgeon water-soluble oil are mixed, put in a 250kg pneumatic extractor, and 150% at 100 to 105 ° C It may be to extract pneumatic hot water for a period of time and put it in a 10L container to mature for 3 months or more.

The aged additional component may be used as an additive including 0.001 to 3% by weight during manufacturing and processing of a dietary beverage composition. These additives, even in small amounts, help to enhance the various effects of the ingredients contained in sturgeon.

As the remaining amount, one or more selected from the above solvents and additives may be included in an appropriate content range.

A specific preparation method for preparing or formulating the dietary composition may follow known techniques commonly used in the art.

Hereinafter, the configuration of the present invention and its effects will be described in more detail through specific examples and comparative examples. However, these examples are for explaining the present invention in more detail, and the scope of the present invention is not limited to these examples.

<Preparation Example 1: Preparation of water-soluble sturgeon oil>

Manufacture of sturgeon raw materials

Farming with eco-friendly non-antibiotics for more than 3 years, feeding clean sturgeon (Siberian sturgeon) with bay salt at least once a month, fasting for 10 days according to the shipping time, and then hitting the head with a rubber mallet once or twice The samples were stunned without pain and immediately quenched and stored. After washing the rapidly frozen sturgeon, the entire sturgeon is put in a pneumatic hot water extractor with 50% sturgeon, 41% water, 5% calamansi undiluted solution, 2% bellflower seed undiluted solution, and 2% beet undiluted solution at 102℃ and 2.5 atm. After 100 hours of extraction, the mixture was separated at the upper, middle, and lower specific gravity points to obtain 80% sturgeon liquid extract, 15% sturgeon solid content, and 5% sturgeon oil, respectively.

Preparation of sturgeon water soluble oil

The obtained sturgeon oil was aged at 17° C. for more than 30 days and then passed through a fine nozzle using a high-pressure pump (high-pressure sprayer) repeatedly dozens of times to foam like fresh cream. This is an operation that causes a change in physical properties to bring it to a state before water-solubility, and after this is completed, it is stored at room temperature for natural aging for another 10 days.

Aged sturgeon oil and water, which have been subjected to the physical natural separation method described above, are mixed in equal proportions (50% aged sturgeon oil + 50% water), put into a distillation extractor, extracted, and returned to the remaining aged sturgeon oil. Only water was added at 120% and extracted by distillation more than 30 times to prepare a first water-soluble oil.

On the other hand, a primary additive was prepared by extracting 50% + 50% water of a mixture of edible snails, Baekbong Ogolgae, white neck mushrooms, beets, kelp roots, and bellflower seeds in a pneumatic hot water extractor for 100 hours. In addition, 50% of a mixture of puerh tea, chrysanthemum, lotus seed, sea bamboo shoot, centella asiatica, wormwood, wormwood, peppermint, yugenpi, hagocho, guccipon, waspong, chaga mushroom, samchae, calamansi and mulberry leaves and water 50% was put into a pneumatic hot water extractor to prepare a secondary additive extracted for 100 hours, and then stirred in a stirrer together with the primary additive.

Then, based on 100 parts by weight of the first additive, 50 parts by weight of the second additive and 50 parts by weight of the first water-soluble oil were placed in a distillation extractor and distilled and extracted to prepare final sturgeon water-soluble oil.

<Preparation Example 2: Preparation of a composition for application to the scalp for mitigating hair loss and promoting hair growth>

Compositions for application to the scalp containing the aforementioned water-soluble sturgeon oil (Example 1: conditioner formulation and Example 2: gel formulation) were prepared in a capacity of 300 ml each.

The conditioner formulation of Example 1 contained 99.1% by weight of sturgeon water-soluble oil and 0.9% by weight of natural fragrance, and the gel formulation of Example 2 contained 98.5% by weight of sturgeon water-soluble oil, 0.4% by weight of chamomer, and 0.4% by weight of algini. % by weight and 0.9% by weight of perfume, and at this time, the specific manufacturing method of the conditioner and gel formulation followed a known technique commonly used in the art.

On the other hand, in Comparative Example 1 and Comparative Example 2, instead of sturgeon water-soluble oil, experimental samples were prepared with conditioner (Comparative Example 1) and gel (Comparative Example 2) formulations, including commercially available scalp application compositions based on purified water, respectively. prepared together.

Referring to Figure 1, it can be seen that the prepared composition for scalp application (Examples 1 and 2 and Comparative Examples 1 and 2) having a transparent appearance.

<Preparation Example 3: Preparation of dietary liquid composition for mitigating hair loss and promoting hair growth>

1 L of a liquid composition for diet containing the above-described water-soluble sturgeon oil at 50% by weight, that is, a beverage composition (Example 3) was prepared in a pouch form.

In the beverage composition, 0.8% by weight of Sanyangsam and Jeokshu root extracts were added as additional components. At this time, the additional ingredients are a mixture of 50% of wild ginseng and red ginseng root mixture and 50% of sturgeon water-soluble oil, put in a 250kg pneumatic extractor, pneumatic hot water extraction at 100 ~ 105 ° C for 150 hours, put in a 10L container and aged for more than 3 months manufactured.

The beverage composition of Example 3 further included commonly used additives such as sweeteners and flavorings, and purified water, and was prepared according to a known technique.

On the other hand, as Comparative Example 3, 1L of a beverage composition containing 50% by weight of a mixed extract of houttuynia cordata, perilla leaf, green tea leaves, and red ginseng, instead of sturgeon water-soluble oil, was prepared. The beverage of Comparative Example 3 also further contained commonly used additives such as sweeteners and flavors, and purified water, and was prepared according to a known technique. Except for the sturgeon water-soluble oil of Example 3 and the mixed extract of Comparative Example 3, all other additives and their contents were the same.

Referring to Figure 2, it can be seen that the beverage composition (Example 3 and Comparative Example 3) prepared in the form of a pouch.

<Experimental Example 1: Cell test>

A cell level test was conducted on the hair growth promoting effect of the sturgeon water-soluble oil prepared according to Preparation Example 1. That is, the cell proliferation promoting effect of the water-soluble sturgeon oil was analyzed in a human follicle dermal philia cell (HFDPC) culture system. The specific test method is as follows.

The cell line used for the cell test was HFDPC (Human Follicle Dermal Papilla Cells) purchased from PromoCell ^ⓡ . The cells were added to a 96 well plate at 2 × 10 ⁴ cells / well, and the water-soluble sturgeon oil of Preparation Example 1 was added to the cell culture medium to a final concentration of 2.5%, 5%, and 10%, respectively, and cultured for 24 hours After that, cell proliferation was evaluated by measuring intracellular dehydrogenase using CCK-8 (Cell counting kit-8).

As a positive control, minoxidil, a hair loss treatment agent, was used, and minoxidil was added to the cell culture medium to a final concentration of 4 μg/ml. The composition of the test group is shown in Table 1 below, and the test results are shown in FIG. 3, respectively.

Referring to Figure 3, in the case of Preparation Example 1, it was confirmed that the proliferation of dermal papilla cells was significantly increased in a concentration-dependent manner.

<Experimental Example 2: Animal Test>

An animal test was conducted on the hair growth promoting effect of the compositions for mitigating hair loss and promoting hair growth, that is, the composition for applying to the scalp and the composition for dietary use, prepared according to Preparation Example 2 and Preparation Example 3, respectively.

Experimental animals used in this test were mice of the C57BL/6 strain. C57BL/6 mice have the characteristic that skin color is determined by the amount of melanin pigment in hair follicles because melanocytes that produce pigment do not exist in the epidermis but only in hair follicles. You can check. In addition, the efficacy of wool can be evaluated by confirming the anagen inducing effect that promotes the transition from telogen to anagen. Human hair has different hair cycles for each individual, but in the case of mice, all hairs are initially in the same hair cycle, and the hair cycles can be matched artificially, so that changes in hair cycles can be compared and observed.

The specific test method is as follows.

Hair follicle telogen screening

Six-week-old C57BL/6 mice were purchased through Samtaco Co., Ltd. and used in the experiment after acclimatization for one week in the Laboratory Animal Center laboratory of Sahmyook University. Mice were put in 2 to 4 mice per cage and raised in an animal breeding chamber at 22 ± 1 ° C and 50% humidity. The weight of the raised mice was measured and divided into 5 experimental groups of 4 mice each so that the average weight of each group was the same.

Then, under anesthesia with respiratory anesthetic (isoflurane), the experimental group mice were removed starting from the lower part of the occipital region with a clipper, and then the hair in an area of about 2 × 3 cm on the back was removed with a clipper, and then a day later, individuals in a dormant hair follicle state were selected. did

sample treatment

First, a test sample was constructed. The compositions of Examples 1 to 3 and Comparative Examples 1 to 3 described above were prepared, respectively, and the experimental group was treated for 4 weeks by combining each composition. Specific test samples and treatment methods (groups 1 to 5) are shown in Table 2 below.

Group 1 was a negative control group, and Comparative Example 1 (purified water conditioner) and Comparative Example 2 (purified water gel) were applied to the hair loss area twice a day for 4 weeks, and Group 2 was a scalp application group with Example 1 (scalp conditioner) Example 2 (scalp gel) was applied to the bald area twice daily for 4 weeks.

Group 3 is a diet and scalp application group, and Comparative Example 3 is fed for 1 to 3 weeks, and in the 4th week, the diet of Comparative Example 3 and the application of Example 1 (scalp conditioner) and Example 2 (scalp gel) are treated together did Group 4 is a diet and scalp application group, and Example 3 is fed for 1 to 3 weeks, and in the 4th week, the diet of Example 3 and the application of Example 1 (scalp conditioner) and Example 2 (scalp gel) are treated together did

Group 5 was a positive control group, and used 1% of hair loss treatment Minoxidil 3% (Hyundai Pharmaceutical) diluted in saline, and was applied to the bald area by about 0.1ml once daily.

In addition, Groups 1, 2, and 5 were allowed to freely consume water, and Groups 3 and 4 were allowed to consume each composition together with water put in a separate water bottle.

During the treatment, the hair growth effect of the scalp for each week was confirmed, and the results are shown in FIG. 4.

Referring to FIG. 4 , in Comparative Examples 1 and 2, which are negative controls, some changes in skin color were confirmed, but it was confirmed that hair did not grow. In Example 1 and Example 2, which are scalp application groups, it was confirmed that skin color change and hair growth progressed at 3 to 4 weeks.

On the other hand, in Group 3, it was confirmed that skin color change and hair growth progressed from the 2nd to 3rd week. In GROUP 4, changes in skin color were confirmed from the 1st to 2nd week, and hair growth was confirmed from the 2nd to 3rd week. In addition, in the positive control group, Minoxidil-treated group, it was confirmed that skin color change and hair growth progressed in 1 to 2 weeks.

Skin tissue analysis (number of hair follicles, hair follicle thickness, dermal thickness)

At the end of the experimental schedule, the skin tissue of the application site of the euthanized mouse was collected, put in a 10% formalin solution to fix the tissue, and slide production was performed. Briefly describing the process, the tissue fixed in formalin was made into a paraffin block and then cut into thin fragments using a microtome, followed by H&E staining. Stained slides were observed for tissue images at 40x magnification using a microscope connected to a computer monitor.

The number of hair follicles was counted through the cross section of the excised tissue, and the hair follicle thickness and dermal thickness were measured using a scale bar in the longitudinal section of the excised tissue. The measurement results are shown in FIGS. 5 to 8 .

5 and 6, it was confirmed that the number of hair follicles in the scalp application group significantly increased compared to the number of hair follicles in the negative control group, and in group 3 and group 4, it was confirmed that the number of hair follicles significantly increased to the same level as the positive control group .

Referring to FIGS. 7 and 8, the hair follicle thickness and dermal thickness are measured using a scale bar in the longitudinal section of the excised tissue, and the hair follicle thickness is clearly increased in all groups 2, 3, 4 and 5 compared to the negative control group. pattern was confirmed. Dermal thickness tended to increase compared to the negative control group in all groups, but no significant difference was identified.

In other words, through Experimental Example 2, which is an animal test, except for the positive control group, changes in skin color and progression of hair growth were confirmed in the order of groups 4, 3, and 2. In addition, a significant increase in the number of hair follicles and the thickness of hair follicles was confirmed in all groups 2, 3, and 4. Thus, it can be interpreted that the hair growth promoting effect of sturgeon water-soluble oil is due to an increase in the number of hair follicles and an increase in hair follicle thickness.

In this specification, only a few examples of various embodiments performed by the present inventors are described, but the technical spirit of the present invention is not limited or limited thereto, and can be modified and implemented in various ways by those skilled in the art, of course.

Claims (3)

In the functional composition for hair loss relief and hair growth containing sturgeon water-soluble oil obtained from whole sturgeon as an active ingredient,
The sturgeon water-soluble oil is obtained from whole sturgeon, and the sturgeon water-soluble oil is contained in an amount of 10 to 99.5% by weight based on the total weight of the composition, and the sturgeon water-soluble oil is dermal papilla (HDP) ) It is characterized in that it promotes the proliferation of cells,
The sturgeon water-soluble oil is prepared by preparing sturgeon oil according to a raw material obtaining method of whole sturgeon; Performing a first aging of the sturgeon oil to prepare an aged sturgeon oil; A pre-treatment step of foaming the aged sturgeon oil by using a high-pressure processor and a fine nozzle to form changes in physical properties of the oil; Second aging of the pretreated sturgeon oil aging product; Mixing the aged sturgeon oil and water, in which the second aging has been performed, and introducing the mixture into a distillation extractor and performing a second extraction; After performing the second extraction, water is mixed with the remaining matured sturgeon oil and introduced into a distillation extractor, and third extraction is performed to produce first water-soluble oil; Preparing a first additive composition containing edible snails and a second additive composition containing Puer tea and chrysanthemum, respectively; and preparing a secondary water-soluble oil by distilling and extracting a mixture of the primary water-soluble oil, the primary additive composition, and the secondary additive composition,
The composition is prepared as a composition for application to the scalp or a liquid composition for diet,
The composition for applying to the scalp further comprises an additional component, which is a mixture of Conarus semidecandrus extract and soybean violet extract, in the range of 0.1 to 5% by weight based on the total weight of the composition,
The dietary liquid composition further comprises an additional component, which is a mixture of wild ginseng extract and rhizome root extract, in the range of 0.001 to 3% by weight based on the total weight of the composition,
The method for preparing the extract of the additional component contained in the dietary liquid composition is to mix 50% of a mixture of wild ginseng and red rhubarb root with 50% of sturgeon oil, put in a 250kg pneumatic extractor, and 150 hours at 100 to 105 ° C. It is characterized in that it is prepared by extracting with pneumatic hot water during extraction and putting it in a 10L container and aging for at least 3 months, and the sturgeon oil is separated from the sturgeon liquid extract obtained according to the raw material obtaining method from the whole sturgeon. Characterized in that, hair loss relief and functional composition for hair growth. delete delete

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