CN114134088A - Choerospondias axillaris fermentation product and preparation method and application thereof - Google Patents

Choerospondias axillaris fermentation product and preparation method and application thereof Download PDF

Info

Publication number
CN114134088A
CN114134088A CN202111656247.5A CN202111656247A CN114134088A CN 114134088 A CN114134088 A CN 114134088A CN 202111656247 A CN202111656247 A CN 202111656247A CN 114134088 A CN114134088 A CN 114134088A
Authority
CN
China
Prior art keywords
parts
choerospondias axillaris
choerospondias
axillaris
fermentation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN202111656247.5A
Other languages
Chinese (zh)
Other versions
CN114134088B (en
Inventor
聂韡
冷军程
马世宏
单承莺
蔡波
束成杰
李卓航
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Guangzhou Opseve Cosmetics Co ltd
NANJING INSTITUTE FOR COMPREHENSIVE UTILIZATION OF WILD PLANTS CHINA COOP
Original Assignee
Guangzhou Opseve Cosmetics Co ltd
NANJING INSTITUTE FOR COMPREHENSIVE UTILIZATION OF WILD PLANTS CHINA COOP
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guangzhou Opseve Cosmetics Co ltd, NANJING INSTITUTE FOR COMPREHENSIVE UTILIZATION OF WILD PLANTS CHINA COOP filed Critical Guangzhou Opseve Cosmetics Co ltd
Priority to CN202111656247.5A priority Critical patent/CN114134088B/en
Publication of CN114134088A publication Critical patent/CN114134088A/en
Application granted granted Critical
Publication of CN114134088B publication Critical patent/CN114134088B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/02—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P39/00—Processes involving microorganisms of different genera in the same process, simultaneously
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Mycology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Gerontology & Geriatric Medicine (AREA)
  • Dermatology (AREA)
  • Epidemiology (AREA)
  • Birds (AREA)
  • Cosmetics (AREA)

Abstract

The invention belongs to the technical field of microbial fermentation, and particularly relates to a choerospondias axillaris fermentation product and a preparation method and application thereof. According to the invention, eurotium cristatum, bacillus subtilis, saccharomyces cerevisiae and aspergillus niger are compounded to obtain a composite microbial inoculum for fermenting choerospondias axillaris, so that the mass percentage of polyphenol in the obtained choerospondias axillaris fermentation product reaches 2.45-3.76%, and DPPH free radicals and ABTS (adenosine triphosphate) are removed by antioxidant activity+The free radicals are respectively increased by 50.47-84.05% and 67.86-103.57%, the moisture retention rate is also remarkably increased, the skin elasticity can be increased, and the cosmetic composition has the effects of oxidation resistance and aging resistance and can be used as a cosmetic ingredient.

Description

Choerospondias axillaris fermentation product and preparation method and application thereof
Technical Field
The invention belongs to the technical field of microbial fermentation, and particularly relates to a choerospondias axillaris fermentation product and a preparation method and application thereof.
Background
The microbial fermentation technology is to utilize microbes to ferment plant raw materials, and is essentially a biotransformation reaction for catalyzing effective components in the plant raw materials by utilizing various enzymes generated in the microbial metabolism process. The microorganism has strong decomposition and transformation capability, and can catalyze and degrade plant cell walls, improve cell membrane permeability and increase dissolution of effective components in plants by using a plurality of extracellular enzymes such as cellulase, ligninase and pectinase generated in the growth and metabolism process; in addition, various extracellular enzymes can degrade macromolecular substances into small molecular substances which are easy to absorb. And the microbial fermentation technology has the characteristics of low cost, no solvent residue, difficult sensitization and the like, becomes a hotspot of current research and development, and is particularly widely applied in the field of development of cosmetic raw materials.
At present, the microbial fermentation has the following problems: (1) the microbial inoculum has low purity, more mixed bacteria, low effective viable count, and unstable reproductive capacity and enzyme production capacity; (2) at present, single strains are mostly adopted, or fermentation is carried out in an open environment, so that the produced enzyme system is single, the quantity and the quality of the strains are uncontrollable, the flavor of the product is monotonous, and the uncontrollable property exists in the product; (3) the natural fermentation is adopted, the influence of environmental factors is great, and the fermentation speed is slow. How to utilize microorganisms to realize the optimization of the activity and the function of a plant fermentation product is a problem to be solved urgently in the microbial fermentation technology industry.
Disclosure of Invention
The invention aims to provide a choerospondias axillaris fermentation product, a preparation method and application thereof, increase the content of active ingredients of the choerospondias axillaris, improve the additional value of the choerospondias axillaris and lay a foundation for the research and development of antioxidant and anti-aging cosmetics.
The invention provides a compound microbial inoculum which comprises the following components in parts by mass: 20-50 parts of Eurotium cristatum, 20-40 parts of Bacillus subtilis, 10-40 parts of Saccharomyces cerevisiae and 10-30 parts of Aspergillus niger.
Preferably, the complex microbial inoculum comprises the following components in parts by mass: 30 parts of eurotium cristatum, 30 parts of bacillus subtilis, 30 parts of saccharomyces cerevisiae and 10 parts of aspergillus niger;
or comprises the following components in parts by mass: 40 parts of eurotium cristatum, 30 parts of bacillus subtilis, 15 parts of saccharomyces cerevisiae and 15 parts of aspergillus niger;
or comprises the following components in parts by mass: 25 parts of eurotium cristatum, 25 parts of bacillus subtilis, 25 parts of saccharomyces cerevisiae and 25 parts of aspergillus niger.
Preferably, the viable bacteria concentration of the eurotium cristatum is 1 x 107cfu/mL; the viable bacteria concentration of the bacillus subtilis is 1 multiplied by 108cfu/mL; the viable bacteria concentration of the saccharomyces cerevisiae is 1 multiplied by 108cfu/mL; the concentration of viable bacteria of the Aspergillus niger is 1 multiplied by 109cfu/mL。
The invention also provides a preparation method of the choerospondias axillaris fermentation product, which comprises the following steps:
mixing the choerospondias axillaris juice with the composite microbial inoculum, and fermenting for 3-15 days at 25-45 ℃, wherein the fermentation liquor contains the choerospondias axillaris fermentation product.
Preferably, the choerospondias axillaris juice is prepared by mixing fresh choerospondias axillaris with water and pulping; the mass ratio of the fresh choerospondias axillaris to the water is 1: 1; the mass ratio of the composite microbial inoculum to the choerospondias axillaris juice is 1-10: 100.
Preferably, the relative humidity of the fermentation is 50% to 90%.
The invention also provides a choerospondias axillaris fermentation product obtained by the preparation method, wherein the mass percentage of polyphenol in the choerospondias axillaris fermentation product is more than or equal to 2.45%.
The invention also provides application of the choerospondias axillaris fermentation product in preparation of cosmetics.
Preferably, the addition amount of the choerospondias axillaris fermentation product in the cosmetic is 2-40 wt.%.
The invention also provides an antioxidant moisturizing cream which comprises 2-40% of axillary choerospondias fruit fermentation product and the balance of auxiliary materials in percentage by mass.
The invention provides a compound microbial inoculum which comprises the following components in parts by mass: 20-50 parts of eurotium cristatum; 20-40 parts of bacillus subtilis; 10-40 parts of saccharomyces cerevisiae and 10-30 parts of aspergillus niger. The eurotium cristatum can secrete a plurality of enzymes in the fermentation process, so that the content of active substances such as polyphenol, flavone, polysaccharide and the like is obviously improved, and the antioxidant activity of a fermentation product is enhanced; the bacillus subtilis can generate enzymes for degrading various complex saccharides, so that substances such as pectin and cellulose are degraded, and various vitamins can be synthesized; the saccharomyces cerevisiae can remove small molecular polysaccharides; aspergillus niger can secrete protease, degrade macromolecular protein, and make it become peptides and amino acids which are favorable for digestion and absorption. The composite microbial inoculum is obtained by mixing the eurotium cristatum, the bacillus subtilis, the saccharomyces cerevisiae and the aspergillus niger in a specific ratio, can greatly improve the content of active ingredients such as polyphenol, flavone and the like in the fermentation process, and can generate other ingredients such as amino acid, vitamin, trace elements and the like which are beneficial to the skin.
The invention utilizes compound bacteriaThe agent is used for fermenting the choerospondias axillaris, the fermentation conditions are strictly controlled, the mass percentage of polyphenol in the obtained choerospondias axillaris fermentation product reaches 2.45-3.76 percent, the content of polyphenol is 1.83-2.81 times of that of choerospondias axillaris juice, and the antioxidant activity is used for removing DPPH free radicals and ABTS+The free radicals are respectively increased by 50.47-84.05% and 67.86-103.57%, the moisture retention rate is also remarkably increased, the skin elasticity can be increased, and the cosmetic composition has the effects of oxidation resistance and aging resistance and can be used as a cosmetic ingredient.
Drawings
In order to more clearly illustrate the technical solution in the embodiments of the present invention, the drawings required to be used in the embodiments will be briefly described below.
FIG. 1 is a graph showing changes in water content of stratum corneum of skin;
FIG. 2 is a graph showing the change in skin moisture loss through skin;
fig. 3 shows the skin elasticity R2 values.
Detailed Description
The invention provides a compound microbial inoculum which comprises the following components in parts by mass: 20-50 parts of eurotium cristatum, 20-40 parts of bacillus subtilis, 10-40 parts of saccharomyces cerevisiae and 10-30 parts of aspergillus niger.
In the invention, the composite microbial inoculum comprises 20-50 parts by weight of eurotium cristatum, preferably 25-45 parts by weight of eurotium cristatum, and further preferably 30-40 parts by weight of eurotium cristatum. In the specific implementation process of the invention, the mass part of the eurotium cristatum can be any value of 20-50, such as 20 parts, 30 parts or 40 parts. The viable bacteria concentration of the eurotium cristatum is 1 multiplied by 107cfu/mL. The eurotium cristatum can secrete a plurality of enzymes in the fermentation process, and the content of active substances such as polyphenol, flavone, polysaccharide and the like is obviously improved, so that the antioxidant activity of the fermentation product is greatly enhanced.
In the invention, the composite microbial inoculum comprises 20-40 parts by weight of bacillus subtilis, preferably 25-35 parts by weight, and further preferably 28-30 parts by weight. In the specific implementation process of the invention, the mass part of the bacillus subtilis can be any value of 20-40, such as 25 parts or 30 parts. Bacillus subtilis of the invention1X 10 of8cfu/mL. The bacillus subtilis can generate enzymes for degrading various complex saccharides, so that substances such as pectin and cellulose are degraded, and various vitamins can be synthesized.
In the invention, the composite microbial inoculum comprises 10-40 parts of saccharomyces cerevisiae, preferably 15-30 parts of saccharomyces cerevisiae, and further preferably 20-25 parts of saccharomyces cerevisiae. In the specific implementation process of the invention, the mass part of the saccharomyces cerevisiae can be any value of 10-40, such as 15 parts, 25 parts or 30 parts. The concentration of viable bacteria of the saccharomyces cerevisiae is 1 multiplied by 108cfu/mL. The saccharomyces cerevisiae can remove micromolecular polysaccharide.
In the invention, the composite microbial inoculum comprises, by mass, 10-30 parts of Aspergillus niger, preferably 15-25 parts, and further preferably 18-20 parts. In the specific implementation process of the invention, the mass part of the aspergillus niger can be any value of 10-30, such as 10 parts, 15 parts or 25 parts. 1 x 10 of Aspergillus niger of the present invention9cfu/mL. The Aspergillus niger of the invention can secrete protease, degrade macromolecular protein and change the macromolecular protein into peptides and amino acids which are beneficial to digestion and absorption.
The invention has no strict requirements on the sources of the eurotium cristatum, the bacillus subtilis, the saccharomyces cerevisiae and the aspergillus niger, can be purchased and obtained, is not limited by manufacturers, and only needs to ensure that the eurotium cristatum, the bacillus subtilis, the saccharomyces cerevisiae and the aspergillus niger are obtained. In the specific implementation process of the invention, the eurotium cristatum, the bacillus subtilis, the saccharomyces cerevisiae and the aspergillus niger are all automatically identified and preserved in a laboratory.
According to the invention, the composite microbial inoculum is obtained by compounding eurotium cristatum, bacillus subtilis, saccharomyces cerevisiae and aspergillus niger according to a certain proportion, the yield of active ingredients such as polyphenol, flavone and the like in the fermentation process can be obviously improved, and other ingredients beneficial to skin such as amino acid, vitamin, trace elements and the like can be generated.
The invention also provides a preparation method of the choerospondias axillaris fermentation product, which comprises the following steps:
mixing the choerospondias axillaris juice with the composite microbial inoculum, and fermenting for 3-15 days at 25-45 ℃, wherein the fermentation liquor contains the choerospondias axillaris fermentation product.
The choerospondias axillaris juice is preferably prepared by mixing fresh choerospondias axillaris and water and pulping. The mass ratio of the fresh choerospondias axillaris to the water is preferably 1: 1. The fresh choerospondias axillaris is preferably cleaned and denucleated. The cleaning and pitting mode of the invention has no strict requirement and can be carried out conventionally. The choerospondias axillaris juice contains 1.34% of polyphenol by mass concentration.
After obtaining the choerospondias axillaris juice, the choerospondias axillaris juice is mixed with the compound microbial inoculum and fermented to obtain the fermentation liquor. The fermentation liquor contains choerospondias axillaris fermentation product. The mass ratio of the complex microbial inoculum to the choerospondias axillaris juice is preferably 1-10: 100, more preferably 2-9: 100, more preferably 3-8: 100, and most preferably 5-6: 100. In the specific implementation process of the invention, the mass ratio of the complex microbial inoculum to the choerospondias axillaris juice can be any value of 1-10: 100, such as 3:100, 5:100 or 8: 100.
In the invention, the fermentation temperature is preferably 25-45 ℃, more preferably 30-42 ℃, and more preferably 37-40 ℃; the fermentation time is preferably 3-15 d, more preferably 5-10 d, and even more preferably 7-8 d; the relative humidity of the fermentation is preferably 50-90%, more preferably 60-80%, and even more preferably 70%.
Before the choerospondias axillaris juice is mixed with the composite microbial inoculum, the choerospondias axillaris juice is preferably sterilized to obtain sterile choerospondias axillaris juice. The temperature for sterilization in the invention is preferably 121 ℃; the time for sterilization is preferably 15-30 min, more preferably 18-28 min, and even more preferably 20-25 min. The sterilization mode is not strictly required, and the sterilization can be carried out by using conventional equipment, such as a sterilization pot.
After the fermentation liquor is obtained, the invention preferably performs separation treatment on the fermentation liquor, and collects the supernatant to obtain the choerospondias axillaris fermentation product. The separation method of the present invention is preferably centrifugation. The invention has no strict requirements on the rotating speed, time and equipment of the centrifugation, and the fermentation supernatant is ensured to be obtained. The obtained choerospondias axillaris fermentation product is light brown transparent liquid, is non-toxic, non-irritant, non-corrosive, stable in physicochemical property and wide in applicable population, does not contain substances harmful to human skin, and belongs to green, safe and environment-friendly raw materials for cosmetics.
The method utilizes the compound microbial inoculum to ferment the choerospondias axillaris juice, strictly controls the fermentation time, temperature and relative humidity, can greatly improve the content of active ingredients such as polyphenol, flavone and the like of the choerospondias axillaris, and can generate other ingredients such as amino acid, vitamin, trace elements and the like which are beneficial to the skin. The results of the examples show that after the fermentation broth obtained by fermentation is separated, the obtained choerospondias axillaris fermentation product contains polyphenol with the mass concentration of 2.45-3.76 percent, which is 1.83-2.81 times of that of choerospondias axillaris juice, and the antioxidant activity of the product can remove DPPH free radicals and ABTS+The free radicals are respectively improved by 50.47-84.05 percent and 67.86-103.57 percent, and the moisture retention rate is also obviously improved.
The invention also provides application of the choerospondias axillaris fermentation product in preparation of cosmetics. In the present invention, the addition amount of the choerospondias axillaris fermentation product in the cosmetic is preferably 2 wt.% to 40 wt.%, more preferably 5 wt.% to 35 wt.%, more preferably 10 wt.% to 30 wt.%, and most preferably 15 wt.% to 25 wt.%. The present invention is not limited to a specific type of the cosmetic, and may be any type of cosmetic, such as water, essence, gel, lotion, cream, or cream. The results of the embodiment of the invention show that the antioxidant moisturizing cream prepared by taking the choerospondias axillaries fermenting product as the main active matter has obvious moisturizing effect, can obviously increase the skin elasticity, and has the effects of resisting oxidation and resisting aging.
When the preparation method is used for preparing water, essence, gel, emulsion, cream or frost, the fermented product of choerospondias axillaris is 2-40% and the balance of auxiliary materials according to the mass percentage. The invention does not limit the specific types and the dosage of the auxiliary materials, and the auxiliary materials are selected conventionally according to the needs.
The invention also provides an antioxidant moisturizing cream which comprises one or more of 16/18 alcohol, glyceryl stearate, caprylic/capric triglyceride, cyclohexasiloxane, white oil, shea butter, carbomer U-20, xanthan gum, glycerol, propylene glycol, butanediol, beta-glucan, allantoin, methylparaben, essence and phenoxyethanol according to the mass percentage. The invention has no strict requirement on the addition of each auxiliary material in the antioxidant moisturizing cream and can meet the requirement of hundred mass percent. The results of the embodiment of the invention show that the antioxidant moisturizing cream provided by the invention can increase the skin elasticity and has the effects of oxidation resistance and aging resistance.
In order to further illustrate the present invention, the following detailed description of the technical solutions provided by the present invention is made with reference to the accompanying drawings and examples, but they should not be construed as limiting the scope of the present invention.
Example 1
Preparation of fermented product of Choerospondias axillaris
Cleaning 1kg of fresh choerospondias axillaris, removing core, adding 1 time of deionized water, pulping to obtain choerospondias axillaris juice, and autoclaving at 121 ℃ for 20min to obtain sterile choerospondias axillaris juice; inoculating 5% composite strain in sterile fructus Choerospondiatis juice, wherein the composite strain contains 30 parts (30 g) of Eurotium cristatum, Bacillus subtilis, Saccharomyces cerevisiae and Aspergillus niger7cfu/mL): 30 parts (30g, 1X 10)8cfu/mL): 30 portions (30g, viable bacteria concentration is 1 multiplied by 108cfu/mL): 10 portions (10g, viable bacteria concentration is 1 multiplied by 10)9cfu/mL). Culturing and fermenting at 30 deg.C and relative humidity of 80% for 10 days; and after the fermentation is finished, centrifuging the mixed fermentation liquor to obtain supernatant, namely the choerospondias axillaris fermentation product.
Example 2
Preparation of fermented product of Choerospondias axillaris
Cleaning 1kg of fresh choerospondias axillaris, removing core, adding 1 time of deionized water, pulping to obtain choerospondias axillaris juice, and autoclaving at 121 ℃ for 15min to obtain sterile choerospondias axillaris juice; inoculating 3% composite strain into sterile choerospondias axillaris juice, wherein the mass component ratio of eurotium cristatum, bacillus subtilis, saccharomyces cerevisiae and aspergillus niger in the composite strain is 40 parts (40g, viable bacteria concentration is 1 multiplied by 10)7cfu/mL): 30 parts (30g, 1X 10)8cfu/mL): 15 parts (15g, 1X 10)8cfu/mL): 15 parts of (15g, alive)The concentration of the bacteria is 1 × 109cfu/mL). Culturing and fermenting at 37 deg.C and relative humidity of 60% for 5 days; and after the fermentation is finished, centrifuging the mixed fermentation liquor to obtain supernatant, namely the choerospondias axillaris fermentation product.
Example 3
Preparation of fermented product of Choerospondias axillaris
Cleaning 1kg of fresh choerospondias axillaris, removing core, adding 1 time of deionized water, pulping to obtain choerospondias axillaris juice, and autoclaving at 121 ℃ for 30min to obtain sterile choerospondias axillaris juice; inoculating 8% composite strain into sterile choerospondias axillaris juice, wherein the composite strain comprises 25 parts (25 g) of Eurotium cristatum, Bacillus subtilis, Saccharomyces cerevisiae and Aspergillus niger in mass ratio7cfu/mL): 25 parts (25g, 1X 10)8cfu/mL): 25 parts (25g, 1X 10)8cfu/mL): 25 portions (25g, viable bacteria concentration is 1 multiplied by 10)9cfu/mL). Culturing and fermenting at 42 deg.C and 70% relative humidity for 7 days; and after the fermentation is finished, centrifuging the mixed fermentation liquor to obtain supernatant, namely the choerospondias axillaris fermentation product.
Comparative example 1
The difference from the example 1 is that after the sterile choerospondias axillaris juice is obtained, the compound microbial inoculum is not added for fermentation, the centrifugation is directly carried out, and the supernatant is taken to obtain the choerospondias axillaris juice supernatant.
Comparative example 2
The same as example 1, except that 5% by mass of eurotium cristatum was inoculated after obtaining sterile choerospondias axillaris juice.
Comparative example 3
The procedure is the same as in example 1, except that 5% by mass of Bacillus subtilis was inoculated after obtaining sterile choerospondias axillaris juice.
Comparative example 4
The difference is that after obtaining sterile choerospondias axillaris juice, 5% of saccharomyces cerevisiae is inoculated.
Comparative example 5
The difference from example 1 is that after obtaining sterile choerospondias axillaris juice, 5% of composite microbial inoculum is inoculated, the mass component ratio of eurotium cristatum and aspergillus niger in the composite microbial inoculum is 30 parts (30g, viable bacteria concentration is 1 multiplied by 107cfu/mL): 10 portions (10g, viable bacteria concentration is 1)109cfu/mL)。
Comparative example 6
The difference from the example 1 is that after the sterile choerospondias axillaris juice is obtained, 5% of composite microbial inoculum is inoculated, the mass component ratio of the bacillus subtilis to the saccharomyces cerevisiae in the composite microbial inoculum is 30 parts (30g, the viable bacteria concentration is 1 multiplied by 10)8cfu/mL): 30 portions (30g, viable bacteria concentration is 1 multiplied by 108cfu/mL)。
Comparative example 7
The difference from example 1 is that after obtaining sterile choerospondias axillaris juice, 5% of composite microbial inoculum is inoculated, the mass component ratio of eurotium cristatum, saccharomyces cerevisiae and aspergillus niger in the composite microbial inoculum is 30 parts (30g, viable bacteria concentration is 1 multiplied by 107cfu/mL): 30 portions (30g, viable bacteria concentration is 1 multiplied by 108cfu/mL): 10 portions (10g, viable bacteria concentration is 1 multiplied by 10)9cfu/mL)。
Comparative example 8
The difference from example 1 is that after obtaining sterile choerospondias axillaris juice, 5% of composite microbial inoculum is inoculated, the mass component ratio of eurotium cristatum, bacillus subtilis and saccharomyces cerevisiae in the composite microbial inoculum is 30 parts (30g, viable bacteria concentration is 1 multiplied by 107cfu/mL): 30 portions (30g, viable bacteria concentration is 1 multiplied by 108cfu/mL): 30 portions (30g, viable bacteria concentration is 1 multiplied by 108cfu/mL)。
Test example 1
Determination of polyphenol content
Gallic acid was used as a standard (purchased from Beijing Zhongke quality inspection Biotechnology Co., Ltd.) and absorbance (A) was measured at a wavelength of 540nm by ferrous tartrate spectrophotometry. Specifically, the method comprises the following steps: deionized water is used for preparing a gallic acid standard solution with the concentration of 0.25mg/mL, the concentration of the gallic acid standard solution is used as an abscissa, the absorbance is used as an ordinate, a regression equation of a standard curve measured at 540nm is 27.13C +0.0027(r is 0.9993), and the contents of polyphenol in the fermented choerospondias axillaries of examples 1 to 3 and comparative examples 2 to 8 and the choerospondias axillaries juice of comparative example 1 are obtained according to the standard curve, and the results are shown in Table 1.
TABLE 1 Polyphenol content in fermented Choerospondias axillaris products of examples 1 to 3 and comparative examples 2 to 8, and Choerospondias axillaris juice of comparative example 1
Figure BDA0003448306120000071
Figure BDA0003448306120000081
As can be seen from Table 1, the mass percentage of polyphenol in the fermented product of choerospondias axillaris reaches 2.45-3.76%, which is 1.83-2.81 times of that of choerospondias axillaris juice, and compared with other microbial inoculum, the composite microbial inoculum provided by the invention can obviously increase the polyphenol content.
Test example 2
Oxidation resistance test
(1) Scavenging ability for DPPH radicals:
preparation of DPPH ethanol solution: accurately weighing DPPH 20mg, adding absolute ethyl alcohol to dissolve and fix the volume in a 250mL volumetric flask to obtain the concentration of 2.0 multiplied by 10-4A DPPH ethanol solution with mol/L; the composition is preserved in dark at 0-4 ℃, can be used as it is when prepared, and is effective within 4 h.
Accurately measuring 1mL of solution to be detected and 1mL of absolute ethanol, respectively mixing with 1mL of DPPH ethanol solution, reacting for 30min, and measuring absorbance at 517nm (respectively marked as A)1、A2) (ii) a 1mL of solution to be detected and 1mL of absolute ethyl alcohol are weighed and mixed uniformly, after reaction for 30min, the absorbance value (marked as A3) is measured at 517 nm. The DPPH clearance of the test solutions was calculated according to the formula and the results are shown in table 2 below.
Clearance rate ═ 1- (A)1-A3)/A2]×100％
Note: the tested solution is the fermented product of choerospondias axillaris of example 1, the fermented product of choerospondias axillaris of example 2, the fermented product of choerospondias axillaris of example 3, the fermented product of choerospondias axillaris of comparative example 1, the juice of choerospondias axillaris of comparative example 1, the fermented product of choerospondias axillaris of comparative example 2, the fermented product of choerospondias axillaris of comparative example 3, the fermented product of choerospondias axillaris of comparative example 4, the fermented product of choerospondias axillaris of comparative example 5, the fermented product of choerospondias axillaris of comparative example 6, the fermented product of choerospondias axillaris of comparative example 7, the fermented product of choerospondias axillaris of comparative example 8 or the positive sample VC(0.05mg/mL)
TABLE 2 DPPH radical scavenging action of Choerospondias axillaris fermentate and Choerospondias axillaris juice
Figure BDA0003448306120000082
Figure BDA0003448306120000091
As can be seen from Table 2, comparative example 1 contains Choerospondias axillaris juice with a higher degree of DPPH radical scavenging than the positive control VCThe clearance rate of DPPH free radicals is obviously improved by the fermented choerospondias axillaris product obtained by fermentation, which not only exceeds the positive control, but also increases 50.47% -84.05% compared with choerospondias axillaris juice. The complex microbial inoculum used by the invention can obviously increase the clearance rate of DPPH free radicals in the fermentation process of the choerospondias axillaries juice.
(2) For ABTS+Scavenging ability of free radical: 5mL of 7mmol/LABTS and 88. mu.L of 140mmol/L potassium persulfate solution were mixed to obtain a stock solution, and the stock solution was allowed to stand overnight and then diluted with absolute ethanol so that the absorbance at 734nm was 0.7. + -. 0.02, which was referred to as a working solution. And (3) adding 4mL of ABTS working solution into 40 mu L of a sample to be detected, shaking and uniformly mixing for 30s, reacting for 6min, measuring absorbance at 734nm, and calculating the clearance rate, wherein the result is shown in the following table 3.
ABTS+Free radical scavenging rate ═ 1-ASample (I)/AWorking fluid)×100％。
Note: a. theSample (I)The fermented product of choerospondias axillaris of example 1, the fermented product of choerospondias axillaris of example 2, the fermented product of choerospondias axillaris of example 3, the fermented product of choerospondias axillaris of comparative example 1, the juice of choerospondias axillaris of comparative example 1, the fermented product of choerospondias axillaris of comparative example 2, the fermented product of choerospondias axillaris of comparative example 3, the fermented product of choerospondias axillaris of comparative example 4, the fermented product of choerospondias axillaris of comparative example 5, the fermented product of choerospondias axillaris of comparative example 6, the fermented product of choerospondias axillaris of comparative example 7, the fermented product of choerospondias axillaris of comparative example 8 or the positive sample VC(0.05mg/mL)。
TABLE 3 ABTS of Choerospondias axillaris fermentation and Choerospondias axillaris juice+Scavenging action of free radical
Figure BDA0003448306120000092
Figure BDA0003448306120000101
As can be seen from Table 3, comparative example 1-Sucus Choerospondiatis to ABTS+Clearance of free radicals than positive control VCLow, but fermented axillary choerospondias axillaris fermentation product, for ABTS+The clearance rate of free radicals is obviously improved and exceeds the positive control, and compared with the choerospondias axillaris juice, the clearance rate is increased by 67.86-103.57%. The compound microbial inoculum used by the invention can obviously increase ABTS in the fermentation process of the choerospondias axillaris juice+Clearance of free radicals. The compound microbial inoculum used in the invention can obviously increase the antioxidant activity in the fermentation process of the choerospondias axillaris juice.
Test example 3
Test of moisturizing Effect
Selecting 13 glass plates (50 × 100mm), respectively sticking 3M adhesive tapes, uniformly coating 100mg of the fermented choerospondias axillaris product of example 1, the fermented choerospondias axillaris product of example 2, the choerospondias axillaris juice of comparative example 1, the fermented choerospondias axillaris product of comparative example 2, the fermented choerospondias axillaris product of comparative example 3, the fermented choerospondias axillaris product of comparative example 4, the fermented choerospondias axillaris product of comparative example 5, the fermented choerospondias axillaris product of comparative example 6, the fermented choerospondias southern example 7, the fermented choerospondias comparative example 8, the fermented choerospondias axillaris product of comparative example 8, 10% of glycerol (positive control) and distilled water (blank control) on the adhesive tapes, accurately weighing the mass of the adhesive tapes, placing the adhesive tapes in a dryer by taking the distilled water as the control (the dryer does not place a potassium acetate saturated solution to control the relative humidity in the vessel to be 60%), drying for 4h and 24h, weighing and calculating the moisture-keeping rate, and obtaining the results shown in the following table 4.
Moisture retention rate is M2/M1×100％
Note: m1: sample weight before drying; m2: sample weight after drying.
TABLE 4 moisturizing rates of Choerospondias axillaris fermentation product and Choerospondias axillaris juice
Figure BDA0003448306120000102
Figure BDA0003448306120000111
As can be seen from Table 4, compared with the axillary choerospondias fruit juice prepared according to the proportion of 1, the axillary choerospondias fruit fermented product is remarkably improved in moisture retention rate, and the growth amount is 39.32% -77.43%. The compound microbial inoculum used by the invention can obviously preserve moisture rate in the fermentation process of the choerospondias axillaris juice.
Example 4
Preparation of antioxidant moisturizing cream
Preparing materials according to the mass percentage: 3.5% 16/18 alcohol, 2.5% glyceryl stearate, 3.0% capric triglyceride, 5.0% cyclohexasiloxane, 1% white oil, 1% shea butter, 0.15% carbomer-20, 0.15% xanthan gum, 5% glycerol, 1% propylene glycol, 1% butylene glycol, 60.85% ionized water, 5% beta-glucan, 0.1% allantoin, 0.1% methylparaben, 10% choerospondias axillaris fermentate of example 1, 0.15% perfume and 0.5% phenoxyethanol.
(specifically: 3.5g of 16/18 alcohol, 2.5g of glyceryl stearate, 3.0g of capric triglyceride, 5.0g of cyclohexasiloxane, 1.0g of white oil, 1.0g of shea butter, 0.15g of carbome-20, 0.15g of xanthan gum, 5.0g of glycerol, 1.0g of propylene glycol, 1.0g of butylene glycol, 60.85g of deionized water, 5.0g of beta-glucan, 0.1g of allantoin, 0.1g of methylparaben, 10.0g of the choerospondias axillaris fermentation product of example 1, 0.15g of essence and 0.5g of phenoxyethanol.)
Mixing 16/18 alcohol, glyceryl stearate, capric triglyceride, cyclohexasiloxane, white oil and shea butter, heating and melting to obtain a first mixture;
heating and stirring carbomer U-20, xanthan gum, glycerol, propylene glycol, butanediol and ionized water, mixing, dissolving carbomer U-20 completely, adding beta-dextran, allantoin and methyl hydroxybenzoate, and stirring to obtain a second mixture;
pouring the second mixture into the first mixture, stirring, homogenizing and emulsifying; and (3) cooling to 50 ℃, adding the axillary choerospondias fruit fermented product, the essence and the phenoxyethanol in the step (1), standing, cooling and filling.
Comparative example 9
Preparing blank antioxidant moisturizing cream: the same as example 4, except that the fermented product of south acid was not contained, and the amount of water added was 70.85%, the other contents were the same.
Test example 4
Evaluation of Effect of antioxidant moisturizing cream
(1) Test of moisturizing Effect
Subject: 15 healthy volunteers aged 18-50 years had no history of skin diseases and cosmetic allergy; the basic value of the moisture content of the skin stratum corneum of the face test area is 15-45. The subjects were asked to cleanse the facial area with a specially provided facial cleanser prior to the test, and to wait at least 20min in the test environment without eating, drinking, and performing vigorous activities during the test.
And (3) testing environment: temperature (20 + -2) deg.C, relative humidity (50% + -10%).
The test method comprises the following steps: after a subject cleans the face with the special facial cleanser, the face is balanced for 20min in a test environment, and the moisture content and the transdermal moisture loss of the skin are measured; samples (example 4 antioxidant moisturizing cream) and blank control area (comparative example 8 blank antioxidant moisturizing cream) were randomly distributed in the facial area of volunteers according to the test area, each area was 3.0cm × 3.0cm, and the sample dosage was (2.0 + -0.1) mg/cm2. Skin stratum corneum moisture content, skin transdermal moisture loss tests were performed near the skin of the subject using the VapoMeter transdermal moisture loss meter (Delfin, finland) and the moistermeter SC skin stratum corneum hydration meter (Delfin, finland) on days 7, 14, 21, 28, 35, 42, 49, 56, and 63 before product use, with the test results shown in fig. 1 and 2.
As can be seen from the trend of the moisture content data in fig. 1, the moisture content of the stratum corneum of the skin of the subject was significantly increased after the antioxidant moisturizer prepared by the present invention was used, and the moisture value of the skin was increased to 57% after 63 days of use and increased to 76% compared to the moisture content before use. Whereas the blank product increased the moisture value of the skin to 41% after 63 consecutive days of use, only about 22% greater than the moisture content before use. The moisturizing effect of the antioxidant moisturizing cream prepared by the invention is 3 times that of the blank antioxidant moisturizing cream in the comparative example 8.
From the change of TEWL in FIG. 2, the subject reduced TEWL from about 22 to about 15 after using the antioxidant moisturizing cream prepared by the present invention, and the reduction amount reached 33%; whereas the TEWL reduction was only about 10% after the blank control was used. According to the data trend line, the antioxidant moisturizing cream prepared by the invention can obviously reduce the TEWL value and obviously enhance the barrier function of skin.
Test example 5
Skin elasticity test
20 crowds with wrinkles at external canthus (with wrinkles on two sides of 1-7 and wrinkles on the left and right sides of the same grade) are selected, the ages of the crowds are 30-60 years, and the proportion of the crowds to the wrinkles is 50% for each of men and women. Any product (cosmetics, external medicines and oral health products) cannot be used 15 days before the tested part. Prior to the test, the test volunteers were asked to wash their face and to sit still 2h after washing in a climate controlled room (22. + -. 1 ℃ C., 50% relative humidity) for more than 20min and to remain relaxed.
In the test, a half-face test is carried out according to a random table, one side is a sample, the other side is a contrast, the skin elasticity of the left and right canthi of a volunteer is tested by an Elastimeter skin elasticity tester (Delfin, Finland), and the average value is taken as the skin elasticity data of the left and right canthi after three tests. After the test is finished, the volunteers use the cream according to the requirements, the sample is smeared on the canthus on one side (example 4 antioxidant moisturizing cream), and the control is smeared on the canthus on the other side (comparative example 8 blank antioxidant moisturizing cream). The test subjects were asked to return visit on days 7, 14, 21, 28, 35, 42, 49, 56 and 63 of the test, and the R2 value for skin elasticity was determined, and the results are shown in FIG. 3.
As is apparent from FIG. 3, the antioxidant moisturizing cream prepared by the invention has very significant effect on canthus skin, and the skin elasticity R2 value shows a significant rising trend along with the increase of the using time, while the blank control has no significant effect on the skin elasticity R2 value. The antioxidant moisturizing cream prepared by the invention can obviously improve the skin elasticity and has an anti-aging effect.
The composite microbial inoculum provided by the invention enables the mass percentage of polyphenol in the obtained choerospondias axillaris fermentation product to reach 2.45-3.76%, and the antioxidant activity of the composite microbial inoculum can remove DPPH free radicals and ABTS+The free radicals are respectively increased by 50.47-84.05% and 67.86-103.57%, the moisture retention rate is also remarkably increased, the skin elasticity can be increased, and the cosmetic composition has the effects of oxidation resistance and aging resistance and can be used as a cosmetic ingredient.
Although the present invention has been described in detail with reference to the above embodiments, it is only a part of the embodiments of the present invention, not all of the embodiments, and other embodiments can be obtained without inventive step according to the embodiments, and the embodiments are within the scope of the present invention.

Claims (10)

1. The composite microbial inoculum is characterized by comprising the following components in parts by mass: 20-50 parts of Eurotium cristatum, 20-40 parts of Bacillus subtilis, 10-40 parts of Saccharomyces cerevisiae and 10-30 parts of Aspergillus niger.
2. The complex microbial inoculant according to claim 1, wherein the complex microbial inoculant comprises the following components in parts by mass: 30 parts of eurotium cristatum, 30 parts of bacillus subtilis, 30 parts of saccharomyces cerevisiae and 10 parts of aspergillus niger;
or comprises the following components in parts by mass: 40 parts of eurotium cristatum, 30 parts of bacillus subtilis, 15 parts of saccharomyces cerevisiae and 15 parts of aspergillus niger;
or comprises the following components in parts by mass: 25 parts of eurotium cristatum, 25 parts of bacillus subtilis, 25 parts of saccharomyces cerevisiae and 25 parts of aspergillus niger.
3. The complex microbial agent of claim 1 or 2, wherein the viable bacteria concentration of the eurotium cristatum is 1 x 107cfu/mL; the viable bacteria concentration of the bacillus subtilis is 1 multiplied by 108cfu/mL; the viable bacteria concentration of the saccharomyces cerevisiae is 1 multiplied by 108cfu/mL; the concentration of viable bacteria of the Aspergillus niger is 1 multiplied by 109cfu/mL。
4. The preparation method of the choerospondias axillaris fermentation product is characterized by comprising the following steps:
mixing the choerospondias axillaris juice with the composite microbial inoculum according to any one of claims 1 to 3, and fermenting for 3 to 15 days at 25 to 45 ℃, wherein the fermentation liquor contains the choerospondias axillaris fermentation product.
5. The method according to claim 4, wherein the Choerospondias axillaris juice comprises mixing fresh Choerospondias axillaris with water, and pulping; the mass ratio of the fresh choerospondias axillaris to the water is 1: 1; the mass ratio of the composite microbial inoculum to the choerospondias axillaris juice is 1-10: 100.
6. The method of claim 4, wherein the fermentation has a relative humidity of 50% to 90%.
7. The fermented choerospondias axillaris product obtained by the preparation method according to any one of claims 4 to 6, wherein the mass percentage of polyphenol in the fermented choerospondias axillaris product is not less than 2.45%.
8. Use of the fermented choerospondias axillaris product according to claim 7 for the preparation of a cosmetic.
9. The use according to claim 8, wherein the choerospondias axillaris fermentation product is added in the cosmetic in an amount of 2 wt.% to 40 wt.%.
10. An antioxidant moisturizing cream is characterized by comprising 2-40% of axillary choerospondias fruit fermentation product and the balance of auxiliary materials according to mass percentage.
CN202111656247.5A 2021-12-31 2021-12-31 Choerospondias axillaris fermentation product and preparation method and application thereof Active CN114134088B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202111656247.5A CN114134088B (en) 2021-12-31 2021-12-31 Choerospondias axillaris fermentation product and preparation method and application thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202111656247.5A CN114134088B (en) 2021-12-31 2021-12-31 Choerospondias axillaris fermentation product and preparation method and application thereof

Publications (2)

Publication Number Publication Date
CN114134088A true CN114134088A (en) 2022-03-04
CN114134088B CN114134088B (en) 2023-10-10

Family

ID=80383946

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202111656247.5A Active CN114134088B (en) 2021-12-31 2021-12-31 Choerospondias axillaris fermentation product and preparation method and application thereof

Country Status (1)

Country Link
CN (1) CN114134088B (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114588091A (en) * 2022-04-21 2022-06-07 加来(济南)生活科技有限公司 Preparation method of Hami melon fermentation raw pulp and application of Hami melon fermentation raw pulp in cosmetics
CN115281297A (en) * 2022-08-09 2022-11-04 西安外事学院 Apple pomace selenium-rich fruit vinegar beverage and preparation method thereof

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105925617A (en) * 2016-06-16 2016-09-07 北京工商大学 Radix pueraiae fermentation protoplasm cosmetic product and preparation method and application thereof
CN107090485A (en) * 2017-05-17 2017-08-25 内蒙古农业大学 A kind of method and its special culture media and complex bacterial agent special for preparing active polysaccharide
CN111821237B (en) * 2020-08-11 2022-03-04 淮阴师范学院 Plant enzyme, preparation method thereof and application of plant enzyme in skin care products
CN112251469A (en) * 2020-10-28 2021-01-22 付鹏磊 Method for improving oxidation resistance of maca by enzymolysis fermentation method
CN113249233B (en) * 2021-06-29 2021-11-09 北京百奥茵诺生物科技有限公司 Eurotium cristatum and microbial inoculum and application thereof

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114588091A (en) * 2022-04-21 2022-06-07 加来(济南)生活科技有限公司 Preparation method of Hami melon fermentation raw pulp and application of Hami melon fermentation raw pulp in cosmetics
CN114588091B (en) * 2022-04-21 2023-09-26 加来(济南)生活科技有限公司 Preparation method of cantaloupe fermentation primary pulp and application of cantaloupe fermentation primary pulp in cosmetics
CN115281297A (en) * 2022-08-09 2022-11-04 西安外事学院 Apple pomace selenium-rich fruit vinegar beverage and preparation method thereof

Also Published As

Publication number Publication date
CN114134088B (en) 2023-10-10

Similar Documents

Publication Publication Date Title
CN114134088A (en) Choerospondias axillaris fermentation product and preparation method and application thereof
CN111643411B (en) Anti-aging composition and application thereof
CN111249218B (en) Saussurea involucrate fermentation stock solution and preparation method and application thereof
CN111603434B (en) Composition for improving skin wrinkles and cosmetic thereof
CN110946813B (en) Compound fermentation extract containing cubilose and preparation method and application thereof
CN106902030A (en) Skin care compositions and cosmetics with moisturizing, smoothing wrinkle and the elastic effect of recovery
CN110368337B (en) Facial mask containing rose hip extract and preparation method
CN109394640B (en) Enzyme skin care composition and application thereof
CN111773134A (en) Moisturizing essence containing DNA sodium and preparation method thereof
CN108852969B (en) Anti-wrinkle anti-aging whitening mask and preparation method thereof
CN114736941A (en) Sodium hyaluronate fermentation product, skin external preparation containing sodium hyaluronate fermentation product, and preparation method and application of sodium hyaluronate fermentation product
CN115998664B (en) Composition containing guava fermentation product and application thereof
CN111973511A (en) Moisturizing composition and skin care product
CN115300437B (en) Moisturizing and anti-aging skin care composition and preparation method and application thereof
CN116637042A (en) Wrinkle-removing anti-aging skin care product and preparation method and application thereof
KR102381645B1 (en) Cosmetic Composition Comprising Poria cocos Fermentation Extract
CN107982189A (en) Anti-wrinkle eye cream containing stem cell secretory factors and preparation method thereof
CN111840165A (en) Preparation method of plant whitening fermentation liquor
TW202114631A (en) Fermentation broth of carica papaya and uses thereof for beautifying skin
CN112190516A (en) Preparation method of plateau barley fermentation liquor
CN111228138A (en) Hyaluronic acid essence and preparation method thereof
CN115252520B (en) Composite plant extract and application thereof
CN112022756A (en) Shower gel containing ganoderma lucidum sporophore spore powder fermentation liquid and preparation method thereof
CN113499298B (en) Cosmetic with whitening and freckle removing synergistic effect and application
CN115364036B (en) Composition containing snail fermentation filtrate and application thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant