CN113662175B - Plant-based enzyme liquid composition for expelling toxin and beautifying and preparation method thereof - Google Patents

Plant-based enzyme liquid composition for expelling toxin and beautifying and preparation method thereof Download PDF

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CN113662175B
CN113662175B CN202110939615.0A CN202110939615A CN113662175B CN 113662175 B CN113662175 B CN 113662175B CN 202110939615 A CN202110939615 A CN 202110939615A CN 113662175 B CN113662175 B CN 113662175B
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CN113662175A (en
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王冠
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Bafeiba Food Technology Co ltd
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Guangzhou Mujia Health Industry Co ltd
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A23V2400/123—Bulgaricus
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A23V2400/125—Casei
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A23V2400/169—Plantarum

Abstract

The invention provides a plant-based enzyme liquid toxin-expelling and beauty-maintaining composition which comprises the following components in parts by weight: 75-85 parts of plant-based enzyme liquid, 2-3 parts of a golden-silk melon seed extract, 5-6 parts of maltodextrin and 3-4 parts of carboxymethyl cellulose; the plant-based enzyme liquid is prepared from plant raw materials, wherein the plant raw materials comprise a basic raw material and an auxiliary raw material in a mass ratio of 80 (0.1-1), and the basic raw material comprises the following raw materials in parts by weight: 18-22 parts of blueberries, 9-11 parts of apples, 9-11 parts of white pears, 9-11 parts of mangoes, 4-6 parts of dragon fruits, 4-6 parts of lemons, 4-6 parts of pineapples, 4-6 parts of red dates and 4-6 parts of cranberries. The invention also provides a preparation method of the plant-based enzyme liquid toxin-expelling and beauty-maintaining composition. The plant-based enzyme liquid toxin expelling and beautifying composition provided by the invention has good toxin expelling and beautifying effects and heat resistance stability.

Description

Plant-based enzyme liquid composition for expelling toxin and beautifying and preparation method thereof
Technical Field
The invention relates to a composition, in particular to a plant-based enzyme liquid toxin-expelling and beauty-maintaining composition and a preparation method thereof.
Background
The ferment is a product obtained by fermenting vegetables, fruits, grains and other plants for a period of time under specific conditions, and the value of the ferment generally depends on the selection of raw materials and the length of the fermentation time. The enzyme exists in the forms of liquid, paste, powder and the like, the best enzyme belongs to enzyme liquid, contains various natural plant essences, does not contain any preservative or pigment, is prepared by fermenting 100% of natural plants, and has the characteristics of high content, strong activity, easier absorption and utilization by human bodies and the like. The ferment liquid has the functions of maintaining the normal functions of the body, promoting metabolism, repairing tissues, purifying blood, etc.
Chinese patent application CN202010264785.9 discloses a composition and a preparation method thereof, wherein the composition comprises the following components in parts by weight: 0.8-1.2 parts of fermented black garlic, 8-12 parts of plant fermentation product, 2.4-3.6 parts of wheat oligopeptide and 96-144 parts of prebiotics, wherein the plant fermentation product comprises AGM ferment liquid. The patent has the problems that the toxin expelling and beautifying effects are general, and the heat resistance stability is not good.
Disclosure of Invention
The invention aims to solve the technical problem of providing a plant-based enzyme liquid toxin-expelling and beautifying composition which has good toxin-expelling and beautifying effects and heat-resisting stability.
In order to solve the technical problems, the technical scheme of the invention is as follows:
the plant-based enzyme liquid toxin-expelling and beauty-maintaining composition comprises the following components in parts by weight: 75-85 parts of plant-based enzyme liquid, 2-3 parts of a golden-silk melon seed extract, 5-6 parts of maltodextrin and 3-4 parts of carboxymethyl cellulose; the plant-based enzyme liquid is prepared from plant raw materials, wherein the plant raw materials comprise a basic raw material and an auxiliary raw material in a mass ratio of 80 (0.1-1), and the basic raw material comprises the following raw materials in parts by weight: 18-22 parts of blueberries, 9-11 parts of apples, 9-11 parts of white pears, 9-11 parts of mangoes, 4-6 parts of dragon fruits, 4-6 parts of lemons, 4-6 parts of pineapples, 4-6 parts of red dates and 4-6 parts of cranberries.
Furthermore, the auxiliary raw materials of the invention are one or more of carrot, cherry, grapefruit, kiwi fruit, Hami melon, papaya, strawberry, hawthorn, passion fruit, orange, tomato, banana, pomegranate, watermelon, lemon, plum, grape, persimmon, lychee, avocado, noni fruit, brown rice, celery, corn, broccoli, asparagus, black fungus, white radish, mung bean, mushroom, taro, white gourd, cabbage, lettuce, swamp cabbage, mushroom, lotus root, lettuce, pumpkin, towel gourd, balsam pear, sweet pepper, carrot, potato, red bean, mung bean, soybean, pea, kidney bean, oat, brown rice, millet, cashew nut, melon seed, peanut, pine nut, almond and walnut.
Further, the golden luffa seed extract is prepared by the following steps:
drying the golden luffa seeds at 60 ℃, crushing, sieving by a 50-mesh sieve to obtain golden luffa seed powder, adding the golden luffa seed powder into an ethanol water solution, heating to 75 ℃, soaking and extracting for 3-4 hours to obtain an extracting solution, centrifuging the extracting solution for 10-20 minutes to obtain a supernatant, carrying out reduced pressure rotary evaporation on the supernatant to 1/10 of the original volume, and carrying out freeze drying to obtain the golden luffa seed extract.
Furthermore, in the preparation steps of the golden luffa seed extract, the volume fraction of the ethanol water solution is 50%, the ratio of the golden luffa seed powder to the ethanol water solution is 1 (25-30) g/mL, and the speed of centrifugal separation is 4000 revolutions per minute.
The invention provides a preparation method of the plant-based enzyme liquid toxin-expelling and beauty-maintaining composition.
In order to solve the technical problems, the technical scheme is as follows:
a preparation method of a plant-based enzyme liquid toxin-expelling and beauty-maintaining composition comprises the following steps:
(1) cleaning the basic raw materials and the auxiliary raw materials, drying in the air, crushing, sieving with a 50-mesh sieve to obtain raw material powder, adding the raw material powder, white sugar, yeast and water into a fermentation tank, sealing, and fermenting at normal temperature for 25-30 days to obtain fermentation liquor I;
(2) pasteurizing the fermentation liquor I obtained in the step (1) for 30-40 minutes, cooling to 30 ℃, inoculating lactic acid bacteria, sealing and fermenting for 50-60 days to obtain fermentation liquor II, and filtering the fermentation liquor II to obtain filtrate, namely the plant-based enzyme solution;
(3) carrying out vacuum rotary concentration on the plant-based enzyme liquid to 30% of the original volume, adding the golden-silk melon seed extract, maltodextrin and carboxymethyl cellulose, uniformly mixing to obtain a mixed liquid, homogenizing the mixed liquid by using a high-pressure homogenizer, and then transferring the homogenized liquid into a spray dryer for spray drying to obtain the plant-based enzyme liquid toxin-expelling and beautifying composition.
Furthermore, in the step (1) of the invention, the weight ratio of the raw material powder, the white sugar, the yeast and the water is 1 (12-14) to 1.2-1.4) to 10.
Further, in the step (2) of the present invention, the pasteurization temperature is 80-90 ℃.
Furthermore, in the step (2) of the invention, the lactobacillus consists of lactobacillus bulgaricus, lactobacillus plantarum and lactobacillus casei, and the weight ratio of the lactobacillus to the first fermentation broth after pasteurization is (0.005-0.007): 1.
Further, in the step (3) of the present invention, the temperature of the high-pressure homogenizer is 30 to 40 ℃ and the pressure is 60 to 80 MPa.
Further, in the step (3) of the invention, the inlet air temperature of the spray dryer is 130-.
Compared with the prior art, the invention has the following beneficial effects:
1) the basic raw materials used by the invention are eight raw materials of blueberry, apple, white pear, mango, dragon fruit, lemon, pineapple, red date and cranberry, and the auxiliary raw materials are more than sixty kinds of plants (including fruits, vegetables, grains, nuts and the like).
2) The golden cucumber (Cucubita pepo L.var. medullosa Alef) is an annual herbaceous plant in the cucurbitaceae, and the golden cucumber seed extract is obtained by extracting golden cucumber seeds, namely golden towel gourd seeds, by using an ethanol aqueous solution as an extraction solvent, contains active ingredients such as polysaccharide and the like, has better heat stability and alkali resistance, and can effectively improve the heat stability and alkali resistance of the composition prepared by the invention.
3) In the step (3) of the preparation method, the mixed solution is subjected to homogenization treatment by using a high-pressure homogenizer, so that the dispersion degree and the combination degree of each component in the composition can be effectively improved, and the toxin expelling and beautifying effects of the composition prepared by the invention are further improved.
Detailed Description
The present invention will be described in detail with reference to specific embodiments, and the exemplary embodiments and descriptions thereof herein are provided to explain the present invention but not to limit the present invention.
Example 1
The plant-based enzyme liquid toxin-expelling and face-beautifying composition comprises the following components in parts by weight: 80 parts of plant-based enzyme liquid, 2.5 parts of a golden-silk melon seed extract, 5.5 parts of maltodextrin and 3.5 parts of carboxymethyl cellulose; the plant-based enzyme liquid is prepared from plant raw materials, wherein the plant raw materials comprise a basic raw material and an auxiliary raw material in a mass ratio of 80:0.8, and the basic raw material comprises the following raw materials in parts by weight: 20 parts of blueberries, 10 parts of apples, 10 parts of white pears, 10 parts of mangos, 5 parts of dragon fruits, 5 parts of lemons, 5 parts of pineapples, 5 parts of red dates and 5 parts of cranberries; the auxiliary raw materials comprise equal weight of carrot, cherry, grapefruit, kiwi fruit, Hami melon, pawpaw, strawberry, hawthorn, passion fruit, orange, tomato, banana, pomegranate, watermelon and lemon.
The golden luffa seed extract is prepared by the following steps:
drying the golden luffa seeds at 60 ℃, crushing, sieving with a 50-mesh sieve to obtain golden luffa seed powder, adding the golden luffa seed powder into 50% ethanol water solution by volume fraction according to the proportion of 1:28g/mL, heating to 75 ℃, soaking and extracting for 3.5 hours to obtain extract, centrifugally separating the extract for 15 minutes at the speed of 4000 revolutions per minute to obtain supernatant, decompressing and rotatably steaming the supernatant to 1/10 of the original volume, and freeze-drying to obtain the golden luffa seed extract.
The preparation method of example 1 comprises the following steps:
(1) cleaning the basic raw materials and the auxiliary raw materials, airing, crushing, sieving by a 50-mesh sieve to obtain raw material powder, adding the raw material powder, white sugar, yeast and water in a weight ratio of 1:13:1.3:10 into a fermentation tank, sealing, and fermenting at normal temperature for 28 days to obtain fermentation liquor I;
(2) pasteurizing the fermentation liquor I obtained in the step (1) at 85 ℃ for 35 minutes, cooling to 30 ℃, inoculating lactobacillus consisting of lactobacillus bulgaricus, lactobacillus plantarum and lactobacillus casei, wherein the weight ratio of the lactobacillus to the pasteurized fermentation liquor I is 0.006:1, hermetically fermenting for 56 days to obtain fermentation liquor II, and filtering the fermentation liquor II to obtain filtrate, namely plant-based enzyme liquid;
(3) carrying out vacuum rotary concentration on the plant-based enzyme liquid to 30% of the original volume, adding the muskmelon seed extract, maltodextrin and carboxymethyl cellulose, uniformly mixing to obtain a mixed liquid, homogenizing the mixed liquid by using a high-pressure homogenizer at the temperature of 35 ℃ and the pressure of 70MPa, transferring the mixed liquid into a spray dryer, and carrying out spray drying to obtain the plant-based enzyme liquid toxin expelling and beautifying composition, wherein the air inlet temperature of the spray dryer is 125 ℃, the air outlet temperature of the spray dryer is 75 ℃, the pressure of the spray dryer is 3.8bar, and the air flow rate of the spray dryer is 700L/H.
Example 2
The plant-based enzyme liquid toxin-expelling and face-beautifying composition comprises the following components in parts by weight: 81 parts of plant-based enzyme liquid, 2.7 parts of a golden-silk melon seed extract, 6 parts of maltodextrin and 3 parts of carboxymethyl cellulose; the plant-based enzyme liquid is prepared from plant raw materials, wherein the plant raw materials comprise a basic raw material and an auxiliary raw material in a mass ratio of 80:0.9, and the basic raw material comprises the following raw materials in parts by weight: 22 parts of blueberries, 9 parts of apples, 9 parts of white pears, 9 parts of mangoes, 4 parts of dragon fruits, 4 parts of lemons, 4 parts of pineapples, 4 parts of red dates and 4 parts of cranberries; the auxiliary raw materials comprise plum, grape, persimmon, litchi, avocado, noni, brown rice, celery, corn, broccoli, asparagus, black fungus, white radish, mung bean and mushroom in equal weight.
The golden luffa seed extract is prepared by the following steps:
drying golden luffa seeds at 60 ℃, crushing, sieving with a 50-mesh sieve to obtain golden luffa seed powder, adding the golden luffa seed powder into 50% ethanol water solution by volume fraction according to the proportion of 1:25g/mL, heating to 75 ℃, soaking and extracting for 4 hours to obtain extract, centrifuging the extract at the speed of 4000 rpm for 10 minutes to obtain supernatant, decompressing and rotatably steaming the supernatant to 1/10 of the original volume, and freeze-drying to obtain the golden luffa seed extract.
The preparation method of example 2 comprises the following steps:
(1) cleaning the basic raw materials and the auxiliary raw materials, airing, crushing, sieving by a 50-mesh sieve to obtain raw material powder, adding the raw material powder, white sugar, yeast and water in a weight ratio of 1:12:1.2:10 into a fermentation tank, sealing, and fermenting at normal temperature for 30 days to obtain fermentation liquor I;
(2) pasteurizing the fermentation liquor I obtained in the step (1) at 90 ℃ for 30 minutes, cooling to 30 ℃, inoculating lactobacillus consisting of lactobacillus bulgaricus, lactobacillus plantarum and lactobacillus casei, wherein the weight ratio of the lactobacillus to the pasteurized fermentation liquor I is 0.006:1, hermetically fermenting for 55 days to obtain fermentation liquor II, and filtering the fermentation liquor II to obtain filtrate, namely plant-based enzyme liquid;
(3) carrying out vacuum rotary concentration on the plant-based enzyme liquid to 30% of the original volume, adding the sweet potato seed extract, maltodextrin and carboxymethyl cellulose, uniformly mixing to obtain a mixed liquid, homogenizing the mixed liquid by using a high-pressure homogenizer at the temperature of 40 ℃ and the pressure of 60MPa, and then transferring the mixed liquid into a spray dryer for spray drying to obtain the plant-based enzyme liquid toxin expelling and beautifying composition, wherein the air inlet temperature of the spray dryer is 130 ℃, the air outlet temperature of the spray dryer is 80 ℃, the pressure of the spray dryer is 3.5bar, and the air flow rate of the spray dryer is 750L/H.
Example 3
The plant-based enzyme liquid toxin-expelling and face-beautifying composition comprises the following components in parts by weight: 85 parts of plant-based enzyme liquid, 3 parts of a golden-silk melon seed extract, 5.2 parts of maltodextrin and 3.6 parts of carboxymethyl cellulose; the plant-based enzyme liquid is prepared from plant raw materials, wherein the plant raw materials comprise a basic raw material and an auxiliary raw material in a mass ratio of 80:1, and the basic raw material comprises the following raw materials in parts by weight: 18 parts of blueberries, 11 parts of apples, 11 parts of white pears, 11 parts of mangoes, 4.5 parts of dragon fruits, 4.5 parts of lemons, 4.5 parts of pineapples, 4.5 parts of red dates and 4.5 parts of cranberries; the auxiliary raw materials comprise taro, wax gourd, Chinese cabbage, lettuce, eggplant, water spinach, mushroom, lotus root, lettuce, pumpkin, towel gourd, balsam pear, pimento, carrot and potato.
The golden luffa seed extract is prepared by the following steps:
drying golden luffa seeds at 60 ℃, grinding, sieving with a 50-mesh sieve to obtain golden luffa seed powder, adding the golden luffa seed powder into 50% ethanol aqueous solution in volume fraction according to the proportion of 1:30g/mL, heating to 75 ℃, soaking and extracting for 3 hours to obtain extract, centrifuging the extract at 4000 rpm for 15 minutes to obtain supernatant, carrying out reduced pressure rotary evaporation on the supernatant to 1/10 of the original volume, and carrying out freeze drying to obtain the golden luffa seed extract.
The preparation method of example 3 comprises the following steps:
(1) cleaning the basic raw materials and the auxiliary raw materials, airing, crushing, sieving by a 50-mesh sieve to obtain raw material powder, adding the raw material powder, white sugar, yeast and water in a weight ratio of 1:14:1.4:10 into a fermentation tank, sealing, and fermenting at normal temperature for 25 days to obtain fermentation liquor I;
(2) pasteurizing the fermentation liquor I obtained in the step (1) at 85 ℃ for 30 minutes, cooling to 30 ℃, inoculating lactobacillus consisting of lactobacillus bulgaricus, lactobacillus plantarum and lactobacillus casei, wherein the weight ratio of the lactobacillus to the pasteurized fermentation liquor I is 0.005:1, sealing and fermenting for 60 days to obtain fermentation liquor II, and filtering the fermentation liquor II to obtain filtrate, namely the plant-based enzyme solution;
(3) carrying out vacuum rotary concentration on the plant-based enzyme liquid to 30% of the original volume, adding the golden-silk melon seed extract, maltodextrin and carboxymethyl cellulose, uniformly mixing to obtain a mixed liquid, homogenizing the mixed liquid by using a high-pressure homogenizer at the temperature of 35 ℃ and the pressure of 75MPa, transferring the mixed liquid into a spray dryer, and carrying out spray drying to obtain the plant-based enzyme liquid toxin expelling and beautifying composition, wherein the air inlet temperature of the spray dryer is 120 ℃, the air outlet temperature of the spray dryer is 70 ℃, the pressure of the spray dryer is 4bar, and the air flow rate of the spray dryer is 800L/H.
Example 4
The plant-based enzyme liquid toxin-expelling and face-beautifying composition comprises the following components in parts by weight: 75 parts of plant-based enzyme liquid, 2 parts of a golden-silk melon seed extract, 5 parts of maltodextrin and 4 parts of carboxymethyl cellulose; the plant-based enzyme liquid is prepared from plant raw materials, wherein the plant raw materials comprise a basic raw material and an auxiliary raw material in a mass ratio of 80:0.1, and the basic raw material comprises the following raw materials in parts by weight: 21 parts of blueberries, 10.5 parts of apples, 10.5 parts of white pears, 10.5 parts of mangoes, 6 parts of dragon fruits, 6 parts of lemons, 6 parts of pineapples, 6 parts of red dates and 6 parts of cranberries; the auxiliary raw materials comprise red beans, mung beans, soybeans, peas, kidney beans, oats, brown rice, millets, cashews, melon seeds, peanuts, pine nuts, almonds and walnuts in equal weight.
The golden luffa seed extract is prepared by the following steps:
drying the golden luffa seeds at 60 ℃, crushing, sieving with a 50-mesh sieve to obtain golden luffa seed powder, adding the golden luffa seed powder into 50% ethanol water solution by volume fraction according to the proportion of 1:27g/mL, heating to 75 ℃, soaking and extracting for 3.6 hours to obtain extract, performing centrifugal separation on the extract at the speed of 4000 revolutions per minute for 20 minutes to obtain supernatant, performing reduced pressure rotary steaming on the supernatant to 1/10 of the original volume, and performing freeze drying to obtain the golden luffa seed extract.
The preparation method of example 4 comprises the following steps:
(1) cleaning the basic raw materials and the auxiliary raw materials, airing, crushing, sieving by a 50-mesh sieve to obtain raw material powder, adding the raw material powder, white sugar, yeast and water in a weight ratio of 1:13:1.2:10 into a fermentation tank, sealing, and fermenting at normal temperature for 27 days to obtain fermentation liquor I;
(2) pasteurizing the fermentation liquor I obtained in the step (1) at 80 ℃ for 40 minutes, cooling to 30 ℃, inoculating lactobacillus consisting of lactobacillus bulgaricus, lactobacillus plantarum and lactobacillus casei, wherein the weight ratio of the lactobacillus to the pasteurized fermentation liquor I is 0.007:1, sealing and fermenting for 50 days to obtain fermentation liquor II, and filtering the fermentation liquor II to obtain filtrate, namely plant-based enzyme solution;
(3) carrying out vacuum rotary concentration on the plant-based enzyme liquid to 30% of the original volume, adding the muskmelon seed extract, maltodextrin and carboxymethyl cellulose, uniformly mixing to obtain a mixed liquid, homogenizing the mixed liquid by using a high-pressure homogenizer at the temperature of 30 ℃ and the pressure of 80MPa, transferring the mixed liquid into a spray dryer, and carrying out spray drying to obtain the plant-based enzyme liquid toxin expelling and beautifying composition, wherein the air inlet temperature of the spray dryer is 125 ℃, the air outlet temperature of the spray dryer is 75 ℃, the pressure of the spray dryer is 3.6bar, and the air flow rate of the spray dryer is 600L/H.
Reference example 1
The difference from example 1 is that: the components do not contain the extract of the golden silk melon seeds, and the preparation steps are omitted.
Reference example 2
The difference from example 1 is that: the preparation step (3) of the composition is changed into: carrying out vacuum rotary concentration on the plant-based enzyme liquid to 30% of the original volume, adding the golden-silk melon seed extract, maltodextrin and carboxymethyl cellulose, uniformly mixing to obtain a mixed liquid, transferring the mixed liquid into a spray dryer, and carrying out spray drying to obtain the plant-based enzyme liquid toxin expelling and beautifying composition, wherein the air inlet temperature of the spray dryer is 125 ℃, the air outlet temperature is 75 ℃, the pressure is 3.8bar, and the air flow rate is 700L/H. Namely, the mixed solution in the step (3) is not subjected to homogenization treatment by using a high-pressure homogenizer.
Comparative example: example 1 of chinese patent application No. CN 202010264785.9.
The first experimental example: toxin expelling and face nourishing effect test
120 women with chloasma at the age of 22-48 years are selected as test objects, the test objects are randomly and averagely divided into 6 groups, each group has 20, and the reference standard of the chloasma is the chloasma diagnosis standard (revised in 2003) revised by the skin disease professional committee of the Chinese and western medicine integration society pigmentary pathology group: the face has the patches with light brown to dark brown and clear boundaries, which are generally distributed symmetrically, and have no inflammation expression and scales, the disease condition can be seasonal, and the condition is light in winter and heavy in summer.
The compositions of examples 1 to 4 of the present invention, reference example 2 and comparative example were administered to 6 groups of test subjects, respectively, by the methods of: 2 g/time, taken in the morning and at night every day, and each time is infused with hot water, and the curative effect is observed after the medicine is continuously taken for 1 month. Observation indexes are as follows: observing and recording the area and the color change of the yellowish-brown spots before and after the test, and calculating the area reduction rate, wherein the area reduction rate is (the area of the chloasma before the test-the area of the chloasma after the test)/the area of the chloasma before the test multiplied by 100 percent. The curative effect standard is as follows: the recovery is realized, the area reduction rate is more than or equal to 90 percent, and the color basically disappears; the effect is obvious, the area reduction rate is more than or equal to 60 percent, and the color is obviously lightened; the method is effective, the area reduction rate is more than or equal to 30%, and the color becomes light; the method is ineffective, the area reduction rate is less than 30 percent, and the color has no obvious change; the total effective rate is counted by cure, obvious effect and effective.
The test results are shown in table 1:
TABLE 1
The number of healed people Number of effective people Number of effective persons Number of invalid persons Total effective rate (%)
Example 1 4 11 4 1 95
Example 2 5 9 4 2 90
Example 3 5 10 3 2 90
Example 4 4 13 2 1 95
Reference example 2 2 8 6 4 80
Comparative example 0 8 4 8 60
As can be seen from Table 1, the total effective rates of the plant-based enzyme solutions of examples 1 to 4 of the invention are obviously higher than those of the comparative examples, which shows that the plant-based enzyme solution toxin expelling and beauty maintaining composition prepared by the invention has better toxin expelling and beauty maintaining effects. The partial preparation steps of reference example 2 are different from those of example 1, and the total effective rate of reference example 2 is reduced compared with that of example 1, which shows that the homogenization treatment of the mixed solution in the preparation step (3) by using a high-pressure homogenizer can effectively improve the toxin expelling and beautifying effects of the composition.
Experiment example two: test for Oxidation resistance
2g of the compositions (examples 1 to 4 and comparative example) were weighed respectively, added to 70% ethanol aqueous solution, mixed uniformly and then fixed to a volume of 25mL to obtain samples. Preparing DPPH solution with the concentration of 0.1mmol/L by using absolute ethyl alcohol, and storing in dark. The absorbance at 519nm of 2mL of water +2mL of DPPH solution, 2mL of sample +2mL of DPPH solution, and 2mL of sample +2mL of absolute ethanol were measured and recorded as A1, A2, and A3, respectively, and then the DPPH radical scavenging rate was calculated:
DPPH radical clearance ═ 1- (a2-A3)/a1 × 100%.
The higher DPPH radical scavenging rate indicates a stronger antioxidant performance of the composition, and the test results are shown in table 2:
TABLE 2
Figure BDA0003214293910000071
Figure BDA0003214293910000081
As can be seen from Table 2, the DPPH free radical clearance rates of the plant-based enzyme solutions of examples 1 to 4 of the invention are obviously higher than those of the comparative examples, which shows that the plant-based enzyme solution toxin-expelling and beauty-maintaining composition prepared by the invention has better antioxidant performance.
Experiment example three: heat resistance stability test
Preparing samples according to the method of the second experimental example (examples 1-4, reference example 1 and comparative example), recording the DPPH free radical clearance rate measured in the second experimental example as B1, then heating the samples to 80 ℃, keeping the temperature for 20 minutes, measuring the DPPH free radical clearance rate again according to the method of the second experimental example as B2, and calculating the retention rate of 1:
the retention ratio was 1 ═ B2/B1X 100%
The higher retention ratio of 1 indicates better heat resistance stability of the composition, and the test results are shown in table 3:
TABLE 3
Retention ratio 1 (%)
Example 1 70.37
Example 2 68.64
Example 3 70.06
Example 4 69.58
Reference example 1 53.45
Comparative example 51.82
As can be seen from Table 3, the retention rate 1 of the plant-based enzyme solutions of examples 1-4 of the present invention is significantly higher than that of the comparative examples, which indicates that the plant-based enzyme solution toxin expelling and beauty maintaining composition prepared by the present invention has good heat stability. The difference of part of components of reference example 1 from example 1 and the much lower retention rate 1 of reference example 1 compared with example 1 indicate that the extract of the sweet potato seeds used in the present invention can effectively improve the heat resistance stability of the plant-based ferment liquid toxin expelling and beautifying composition.
Experimental example four: alkali resistance test
Samples (examples 1 to 4, reference example 1 and comparative example) were prepared according to the method of the second experimental example, the DPPH radical scavenging rate measured in the second experimental example was recorded as B1, the pH of the sample was adjusted to 9.0 with a sodium hydroxide solution having a concentration of 0.1mol/L, the DPPH radical scavenging rate was measured again according to the method of the second experimental example and recorded as B3, and the retention ratio was calculated as 2:
the retention ratio was 2 ═ B3/B1X 100%
The higher retention 2 indicates better alkali resistance of the composition, and the test results are shown in table 4:
TABLE 4
Retention ratio 2 (%)
Example 1 78.46
Example 2 76.75
Example 3 76.18
Example 4 77.39
Reference example 1 59.87
Comparative example 56.64
As can be seen from Table 4, the retention rate 2 of the plant-based enzyme solutions of examples 1-4 of the present invention is significantly higher than that of the comparative examples, which indicates that the plant-based enzyme solution toxin expelling and skin caring composition prepared by the present invention has good alkali resistance. Compared with example 1, the retention rate 2 of reference example 1 is greatly reduced compared with example 1 due to the difference of part of components of reference example 1, which shows that the sweet potato seed extract used in the invention can effectively improve the alkali resistance of the plant-based enzyme solution toxin expelling and beautifying composition.
The foregoing embodiments are merely illustrative of the principles and utilities of the present invention and are not intended to limit the invention. Any person skilled in the art can modify or change the above-mentioned embodiments without departing from the spirit and scope of the present invention. Accordingly, it is intended that all equivalent modifications or changes which can be made by those skilled in the art without departing from the spirit and technical spirit of the present invention be covered by the claims of the present invention.

Claims (8)

1. A plant-based enzyme liquid composition for expelling toxin and beautifying features that: the paint comprises the following components in parts by weight: 75-85 parts of plant-based enzyme liquid, 2-3 parts of a golden-silk melon seed extract, 5-6 parts of maltodextrin and 3-4 parts of carboxymethyl cellulose; the plant-based enzyme liquid is prepared from plant raw materials, wherein the plant raw materials comprise a basic raw material and an auxiliary raw material in a mass ratio of 80 (0.1-1), and the basic raw material comprises the following raw materials in parts by weight: 18-22 parts of blueberries, 9-11 parts of apples, 9-11 parts of white pears, 9-11 parts of mangoes, 4-6 parts of dragon fruits, 4-6 parts of lemons, 4-6 parts of pineapples, 4-6 parts of red dates and 4-6 parts of cranberries; the auxiliary raw materials are one or more of carrot, cherry, grapefruit, kiwi fruit, Hami melon, pawpaw, strawberry, hawthorn, passion fruit, orange, tomato, banana, pomegranate, watermelon, lemon, plum, grape, persimmon, lychee, avocado, noni, brown rice, celery, corn, broccoli, asparagus, black fungus, white radish, mung bean, mushroom, taro, white gourd, Chinese cabbage, lettuce, eggplant, swamp cabbage, mushroom, lotus root, lettuce, pumpkin, towel gourd, balsam pear, sweet pepper, potato, red bean, soybean, pea, kidney bean, oat, rice, millet, cashew nut, melon seed, peanut, pine nut, almond and walnut;
the golden luffa seed extract is prepared by the following steps:
drying the golden luffa seeds at 60 ℃, crushing, sieving with a 50-mesh sieve to obtain golden luffa seed powder, adding the golden luffa seed powder into an ethanol water solution, heating to 75 ℃, soaking and extracting for 3-4 hours to obtain an extracting solution, centrifugally separating the extracting solution for 10-20 minutes to obtain a supernatant, carrying out reduced pressure rotary steaming on the supernatant to 1/10 of the original volume, and carrying out freeze drying to obtain the golden luffa seed extract.
2. The plant-based enzyme liquid toxin expelling and beautifying composition according to claim 1, which is characterized in that: in the preparation steps of the golden luffa seed extract, the volume fraction of the ethanol water solution is 50%, the proportion of the golden luffa seed powder to the ethanol water solution is 1 (25-30) g/mL, and the centrifugal separation speed is 4000 r/min.
3. The method for preparing the toxin-expelling and beauty-maintaining plant-based enzyme solution composition according to any one of claims 1-2, wherein the method comprises the following steps: the method comprises the following steps:
(1) cleaning the basic raw materials and the auxiliary raw materials, drying in the air, crushing, sieving with a 50-mesh sieve to obtain raw material powder, adding the raw material powder, white sugar, yeast and water into a fermentation tank, sealing, and fermenting at normal temperature for 25-30 days to obtain fermentation liquor I;
(2) pasteurizing the fermentation liquor I obtained in the step (1) for 30-40 minutes, cooling to 30 ℃, inoculating lactic acid bacteria, sealing and fermenting for 50-60 days to obtain fermentation liquor II, and filtering the fermentation liquor II to obtain filtrate, namely the plant-based enzyme solution;
(3) carrying out vacuum rotary concentration on the plant-based enzyme liquid to 30% of the original volume, adding the golden-silk melon seed extract, maltodextrin and carboxymethyl cellulose, uniformly mixing to obtain a mixed liquid, homogenizing the mixed liquid by using a high-pressure homogenizer, and then transferring the homogenized liquid into a spray dryer for spray drying to obtain the plant-based enzyme liquid toxin-expelling and beautifying composition.
4. The preparation method of the plant-based enzyme solution toxin expelling and beauty maintaining composition according to claim 3, wherein the preparation method comprises the following steps: in the step (1), the weight ratio of the raw material powder, the white sugar, the yeast and the water is 1 (12-14) to 1.2-1.4) to 10.
5. The preparation method of the plant-based enzyme solution toxin expelling and beauty maintaining composition according to claim 3, wherein the preparation method comprises the following steps: in the step (2), the temperature during pasteurization is 80-90 ℃.
6. The preparation method of the plant-based enzyme solution toxin expelling and beauty maintaining composition according to claim 3, wherein the preparation method comprises the following steps: in the step (2), the lactobacillus consists of lactobacillus bulgaricus, lactobacillus plantarum and lactobacillus casei, and the weight ratio of the lactobacillus to the pasteurized fermentation liquor I is (0.005-0.007): 1.
7. The preparation method of the plant-based enzyme solution toxin expelling and beauty maintaining composition according to claim 3, wherein the preparation method comprises the following steps: in the step (3), the temperature of the high-pressure homogenizer is 30-40 ℃, and the pressure is 60-80 MPa.
8. The preparation method of the plant-based enzyme solution toxin expelling and beauty maintaining composition according to claim 3, wherein the preparation method comprises the following steps: in the step (3), the inlet air temperature of the spray dryer is 120-130 ℃, the outlet air temperature is 70-80 ℃, the pressure is 3.5-4bar, and the air flow rate is 600-800L/H.
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