CN102349865B - Green bean sprout extract and preparation method and use thereof - Google Patents
Green bean sprout extract and preparation method and use thereof Download PDFInfo
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- CN102349865B CN102349865B CN 201110300908 CN201110300908A CN102349865B CN 102349865 B CN102349865 B CN 102349865B CN 201110300908 CN201110300908 CN 201110300908 CN 201110300908 A CN201110300908 A CN 201110300908A CN 102349865 B CN102349865 B CN 102349865B
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Abstract
The invention discloses a green bean sprout extract and a preparation method and a use thereof. The green bean sprout is simple and convenient to extract and separate, and the obtained product has a high purity, a high extraction ratio of active ingredients, small loss and a greatly-improved product quality. During the germination process of the green bean, the insoluble substance of a high molecular weight is converted into soluble substance of a small molecular weight; the stored substance is converted into physiological bioactive substances; the content of amino acid and various vitamins are also improved greatly; and the changes of these active ingredients further enhance the efficiency of the green bean sprout extract serving as cosmetics, so that the efficiency of the green bean sprout extract for cleaning skin, improving dry skin, maintaining skin elasticity, relieving and resisting sensitivity, and removing acne is more remarkable.
Description
Technical field
The present invention relates to a Plant Extracts, specifically a kind of Mung Bean extract and preparation method thereof and new purposes.
Background technology
Semen phaseoli radiati has another name called Semen phaseoli radiati, is the mature seed of leguminous herbaceous plant's Semen phaseoli radiati, in China's cultivation history of existing more than 2,000 year, in various places, plantation is arranged as cereal crops.Rich in proteins, carbohydrate, each seed amino acid and trace element in Semen phaseoli radiati, wherein bioactive substance mainly contains Flavonoid substances, SOD, tannin, coumarin, alkaloid, plant sterol etc., has the effects such as antioxidation, antitumor, blood fat reducing, raising immunity.The successive dynasties book on Chinese herbal medicine is described the medicinal efficacy of Semen phaseoli radiati more, and " Semen phaseoli radiati though the merit of detumescence treatment of smallpox is same as Semen Phaseoli, and presses the power of thermal detoxification to cross it to the Compendium of Material Medica cloud.And QI invigorating, thick the intestines and stomach, qualcomm meridian are without taking for a long time avoiding of withered people.Surgery is controlled carbuncle, has expelling the toxic substance from the body with tonic to protect the heart loose, and the utmost point is sayed its effect." and can " separate metal and stone, arsenicum, all poison of vegetation "." book on Chinese herbal medicine is looked for the truth " day: " the Semen phaseoli radiati sweet in the mouth is cold in nature, claims to be apt to according to the standby utmost point of book, have speech can thick the intestines and stomach, profit skin and the five internal organs and money taste, though by this with join, luxuriant, return, art, be not yet.The the institute says can be thick, can moisten, can and, can money person, edge is vehement in evil because of poison, all internal organs meridians skin taste, none is not disturbed by poison, take this property and be apt to detoxify, therefore all invariably prove effective with this.”
At present, Semen phaseoli radiati is multiplex in the preparation of health food, and its purposes and effect are more single, and how development period, potential using value was the focus that those skilled in the art study.
Summary of the invention
The object of the invention is to propose a kind of Mung Bean extracting method.
A further object of the present invention is to propose a kind of Mung Bean extract that utilizes said method to extract and obtain.
The 3rd purpose of the present invention is to propose the purposes of above-mentioned Mung Bean extract.
Invention thinking of the present invention is: Semen phaseoli radiati is after germinateing, its active component is further improved, the insoluble matter of the macromolecule in Semen phaseoli radiati transforms to the soluble substance of small-molecular weight, storage class material transforms to biological active substances, the content of aminoacid, various vitamin also significantly improves, its effect that is used for cosmetics has further been established in the variation of these active component, makes its cleaning skin, improves xerosis cutis, keeps skin elastic force, Shu Min antiallergic effect, acne-removing be more remarkable.In recent years, sudden strain of a muscle formula extraction method have room temperature extract, without composition destroy, the advantage such as quick, energy-efficient simple to operate, be widely used in the extraction of various compositions in various raw materials (comprising root, stem, leaf, flower, fruit, seed of plant etc.), and can have adopted multi-solvents to extract.Therefore adopt sudden strain of a muscle formula extraction equipment to carry out extract at room temperature to Semen Phaseoli radiati Germinatus, can farthest reduce the destruction of effective ingredient and improve extraction ratio.
To achieve these goals, the present invention adopts following concrete technical scheme:
A kind of extracting method of Mung Bean, described method step is as follows:
(1) get the rudiment Semen Phaseoli radiati Germinatus of two to four days, add deionized water according to the weight ratio of 1: 2～1: 4, carry out extract at room temperature with flash extracter, extraction time is 3～5 minutes, and speed is 4000～6000r/min;
Filter, centrifugal, get supernatant, supernatant adds the 1,3 butylene glycol of 10wt%～20wt%, is mass ratio herein, and purpose is for forming stabilising system, prolection material to a certain extent.
Obtain the rudiment Semen phaseoli radiati extract after (2) 80～90 ℃ of high temperature sterilizes, the high temperature sterilize time is recommended as 20～30 minutes;
Being filtered into 200～400 orders in described step (2) filters.
In described step (2), centrifugal rotational speed is 4000～5000rpm, preferred 5000rpm; The centrifugal time is 10～20 minutes, preferred 20 minutes.
The described rudiment Semen Phaseoli radiati Germinatus of two to four days is prepared by following method:
(1) Semen phaseoli radiati is cleaned up, soak 6～10h;
(2) Semen phaseoli radiati after soaking was sprouted 2～4 days under 20 ℃～25 ℃, obtained described Semen Phaseoli radiati Germinatus.
Semen phaseoli radiati after soaking in described step (2) first drains away the water and is placed in and is lined with the cotton container of moistening dehydration, and covers with gauze, is placed in to cultivate under sprouting under 20 ℃～25 ℃ and sprouts; At regular intervals, be generally 2 hours, trickle once keeps Semen phaseoli radiati moistening, and every 18～30h cleans once with clear water, prevents that Semen Phaseoli radiati Germinatus is mouldy rotten; Germinating time is 2～4 days, after the end of germinateing, Semen Phaseoli radiati Germinatus is cleaned at least one times with clear water.
Semen phaseoli radiati all can germinate under these conditions, though the germination degree, as long as select the germination Semen phaseoli radiati to extract.
A kind of Mung Bean extract, it is obtained by the said method extraction.
The application of described Mung Bean extract in the skin care item of preparation moisturizing, Shu Min antiallergic, acne-removing.
A kind of skin care item with moisturizing, Shu Min antiallergic, acne-removing, institute's skin care item are made by above-mentioned Mung Bean extract and the acceptable adjuvant of skin care field.
Different according to the use approach, Mung Bean extract of the present invention is made the external use skin care preparation, as external facial cream, cosmetic water, cleansing milk, spray, facial film or essence etc.But be not limited to above-mentioned dosage form, can be according to method known in the skin care item industrial circle, the said extracted thing is made various external preparation after by the known method degerming.
Beneficial effect of the present invention:
The invention provides a kind of extracting method for Mung Bean, the method is extracted the Mung Bean extract that obtains and is had very high external-use skin care value, and especially anti-acne antiallergic moisture-keeping efficacy is remarkable.The extraction separation method that provides is easy, and products obtained therefrom purity is higher, and effective component extraction rate is high, loses littlely, and product quality is high.In the process of Mung Bean Germination, the insoluble matter of macromolecule transforms to the soluble substance of small-molecular weight, storage class material transforms to biological active substances, the content of aminoacid, various vitamin also significantly improves, its effect that is used for skin care item has further been established in the variation of these active component, makes its cleaning skin, improves xerosis cutis, keeps skin elastic force, Shu Min antiallergic effect, acne-removing be more remarkable.By the method for rudiment, further improved the content and the quality of having improved active component of active component.Resulting extract has the effect of stronger moisturizing, Shu Min antiallergic, anti-acne through verification experimental verification.Therefore the rudiment Semen phaseoli radiati extract can be used as the functional additive of moisturizing, Shu Min antiallergic, anti-acne, and in the preparation skin care item, especially preparation has the cosmetics of Shu Min antiallergic, acne-removing, has extensive use.
Description of drawings
Fig. 1 is the experiment of Mung Bean extract hyaluronidase inhibitor;
Fig. 2 is that the rudiment Semen phaseoli radiati extract is to propionibacterium acnes fungistatic effect figure.
The specific embodiment
Below by specific embodiment, method of the present invention is described, but the present invention is not limited thereto.Experiment detection method described in following embodiment if no special instructions, is conventional method; Described reagent and material if no special instructions, all can obtain from commercial channels.
Specific embodiment of the invention Semen phaseoli radiati used is commercially available Semen phaseoli radiati, without specific (special) requirements.Flash extracter used is the JHBC-50S type flash extracter that Henan gold ancient cooking vessel development in science and technology company limited is sold, the flash extracter that other manufacturer production are sold all can be used for the present invention, as long as preparation method and conditional parameter meet scope of the present invention, can obtain effect of the present invention.
The concrete preparation process of rudiment Semen phaseoli radiati extract is as follows:
(1) preparation rudiment Semen phaseoli radiati
Get the Semen phaseoli radiati of certain mass, clean up, add deionized water, the mass ratio of water and Semen phaseoli radiati is 2: 1, soaks 8h under room temperature, after soaking end, Semen phaseoli radiati being drained away the water and be placed in is lined with the cotton container of moistening dehydration, and covers with gauze, is placed in that under room temperature, the best is to cultivate under 22 ℃ to sprout; Every 2 hours trickles once, keep Semen phaseoli radiati moistening, every 24h cleans once with clear water, prevents that the bean sprout is mouldy rotten; Germinate and after 3 days, the bean sprout is cleaned once with clear water, take out.
(2) extract
Get the Semen Phaseoli radiati Germinatus of rudiment about three days, add deionized water at 1: 2 according to weight ratio, use the JHBC-50S flash extracter, carry out extract at room temperature; 3 minutes extraction times, extraction rate is controlled at 4000r/min;
200 mesh filter screens filter, and centrifugal, centrifugal rotational speed is 5000r/min, centrifugal 20 minutes; Get supernatant, add 10% 1,3 butylene glycol, obtain the rudiment Semen phaseoli radiati extract after 85 ℃ of high temperature sterilize 20min.
(1) preparation rudiment Semen phaseoli radiati
Get the Semen phaseoli radiati of certain mass, clean up, add deionized water, the mass ratio of water and Semen phaseoli radiati is 1.5: 1, soaks 10h under room temperature, after soaking end, Semen phaseoli radiati being drained away the water and be placed in is lined with the cotton container of moistening dehydration, and covers with gauze, is placed in to cultivate under 20 ℃ and sprouts; Every 1.5 hours trickles once, keep Semen phaseoli radiati moistening, every 30h cleans once with clear water, prevents that the bean sprout is mouldy rotten; Germinate and after 2 days, the bean sprout is cleaned once with clear water, take out.
(2) extract
Get the Semen Phaseoli radiati Germinatus of rudiment about three days, add deionized water at 1: 3 according to weight ratio, use the JHBC-50S flash extracter, carry out extract at room temperature; 5 minutes extraction times, extraction rate is controlled at 5000r/min; 200 mesh filter screens filter, and centrifugal, centrifugal rotational speed is 4000r/min, centrifugal 10 minutes; Get supernatant, add 15% 1,3 butylene glycol, obtain the rudiment Semen phaseoli radiati extract after 80 ℃ of high temperature sterilize 30min.
(1) preparation rudiment Semen phaseoli radiati
Get the Semen phaseoli radiati of certain mass, clean up, add deionized water, the mass ratio of water and Semen phaseoli radiati is 3: 1, soaks 6h under room temperature, after soaking end, Semen phaseoli radiati being drained away the water and be placed in is lined with the cotton container of moistening dehydration, and covers with gauze, is placed in to cultivate under 25 ℃ and sprouts; Every 1 hour trickle once, keep Semen phaseoli radiati moistening, every 18h cleans once with clear water, prevents that the bean sprout is mouldy rotten; Germinate and after 4 days, the bean sprout is cleaned once with clear water, take out.
(2) extract
Get the Semen Phaseoli radiati Germinatus of rudiment about three days, add deionized water at 1: 4 according to weight ratio, use the JHBC-50S flash extracter, carry out extract at room temperature; 4 minutes extraction times, extraction rate is controlled at 6000r/min; 200 mesh filter screens filter, and centrifugal, centrifugal rotational speed is 5000r/min, centrifugal 15 minutes; Get supernatant, add 20% 1,3 butylene glycol, obtain the rudiment Semen phaseoli radiati extract after 90 ℃ of high temperature sterilize 25min.
The preparation of embodiment 4 emulsions of the present invention
1, raw material and consumption:
A phase: cetearyl alcohol 5wt%
Behenyl alcohol 2wt%
The two stearate 2wt% of tetramethylolmethane
Glyceryl monostearate 2wt%
Shea butter 5wt%
Dimethicone 3wt%
B phase: cetearyl glucoside 3wt%
Carbomer 0.1wt%
Disodium EDTA 0.15wt%
The rudiment Semen phaseoli radiati extract 5wt% of embodiment 1 preparation
C phase: antiseptic 0.5wt%
Essence 0.2wt%
Collagen protein 3wt%
10％NaOH 0.5wt％
Surplus is deionized water.
2, preparation method:
(1) take each raw material by above-mentioned component and consumption;
(2) the A phase being heated to respectively 80 ℃ of stirrings mutually with B makes material dissolution complete;
(3) A is added in the emulsifying pot stirs, stir speed (S.S.) is 80 rev/mins, B is added in the emulsifying pot while stirring, the speed that adds is as the criterion with the whole emulsifyings of B phase raw material in the emulsifying pot, evacuation after B phase raw material all adds (vacuum is-0.1 atmospheric pressure), stirring homogenizing 4 minutes, stir speed (S.S.) is 80 rev/mins, be heated to 80 ℃ of insulations, begin cooling after 20 minutes, when dropping to 30 ℃, temperature adds C phase raw material, stir and continue cooling, when temperature during at 40 ℃ homogenizing discharging in 1～2 minute get final product.
The functional examination test of embodiment 5 rudiment Semen phaseoli radiati extracts
The rudiment Semen phaseoli radiati extract for preparing in embodiment 1,2,3 is carried out respectively following experiment successively.
Rudiment Semen phaseoli radiati extract solution used in following test, be that the rudiment Semen phaseoli radiati extract solution that embodiment 1,2,3 prepares is designated as mass concentration is 100% extract solution, the extract solution of other concentration carries out corresponding dilution with this solution and obtains.
1, the moisturizing of Mung Bean extract experiment
This experiment of 30 equal voluntary participations of volunteer.
Reflecting cuticular barrier function through epidermis water loss value (TEWL), is the important indicator of estimating the horny layer state.Protective layer of skin is more intact, and the numerical value of its TEWL is just lower.Use moisture of skin loss tester (Tewameter TM300) to measure near the water vapor pressure gradient of epidermis, can obtain numerical value, the curve of TEWL, and the data such as meansigma methods and standard deviation, the cosmetics moisture-keeping efficacy is estimated.
The volunteer (30, man: 15; Female: 15) before the test, enter laboratory half an hour, sits quietly 30 minutes time.As test zone, smear the common skin cream of the basic nursing of adding the rudiment extract with left arm; The corresponding symmetrical region of right arm is smeared the common skin cream of the basic nursing of not adding the rudiment extract, as blank.Detect the moisture of skin number of dropouts of each test zone with the moisture of skin tester (Tewameter TM300) that runs off, detect each duplicate detection 3 times in smearing rear different time.The measured value that the meansigma methods that the meansigma methods that each time point is detected deducts blank is this this position of period is the TEWL value added, and the meansigma methods that each time point is detected is the TEWL rate of change at this this position of period divided by the meansigma methods of blank.
The results are shown in Table 1, as can be seen from the table, after smearing, human body skin moisture flow vector reduces, and shows that the Mung Bean extract has certain moisture-keeping efficacy.
Table 1TEWL testing result
| Smear the rear time | TEWL rate of change (%) |
| 30min | -3.58 |
| 60min | -6.95 |
| 90min | -7.02 |
| 120min | -6.47 |
| 180min | -8.77 |
| 240min | -7.21 |
2, Mung Bean extract hyaluronidase inhibitor experiment
The method is carried out according to the method that provides as Publication about Document: Huang Li, Feng Zhichen, Wei Baoyao, Teng Jianwen.The research of Radix Sanguisorbae and Ramulus Cinnamomi anti-allergic effects, food science and technology, the 06th phase: 135-137 in 2007.
Hyaluronidase is anaphylactoid participant, studies show that hyaluronidase and inflammation, allergy have strong correlation, and many Claritins have the effect of strong hyaluronidase inhibitor.The hyaluronic acid endonuclease capable is without selectively being hydrolyzed 1 between β-N-acetyl-glucosamine in hyaluronic acid and D-glucuronate, the 4-key, and obtain β-N-acetyl-glucosamine, β-N-acetyl-glucosamine under alkali condition can with the acetylacetone,2,4-pentanedione condensation former 2-methyl-3-diacetyl azole derivatives that adds lustre to, the former and p-dimethylaminobenzaldehyde that adds lustre to adds lustre in concentrated hydrochloric acid ethanol.The fundamental reaction equation:
2-methyl-3-acetyl-azole derivatives+P-DAB → add lustre to (structure is not clear)
Experimental technique: get 0.1mL 0.25mmol/L CaCl
2Solution and 37 ℃ of heat insulating culture 20min of 0.5mL hyaluronidase liquid; Add respectively sample liquid ( embodiment 1,2,3 extracts) 0.5mL, continue 37 ℃ of heat insulating culture 20min; Add 37 ℃ of insulation 30min of 0.5mL hyaluronate sodium liquid, room temperature is placed 5min; Add 0.1mL0.4mol/L NaOH solution and 0.5mL acetylacetone,2,4-pentanedione solution, be placed in and carry out cooling 5min with frozen water immediately after boiling water bath heats 15min; Add Ehrlich's reagent 1.0mL and dilute with the 3.0mL dehydrated alcohol, placing the 20min colour developing, with its absorbance of spectrophotometric determination.
The measure and calculation formula of sample to the hyaluronic acid enzyme inhibition rate:
A in formula---contrast solution ABS value (replacing sample solution with hac buffer)
B---contrast blank solution ABS value (replacing sample solution and enzyme liquid with hac buffer)
C---sample solution ABS value
D---sample blank solution A BS value (replacing enzyme liquid with hac buffer)
Experimental result is seen Fig. 1, and as can be seen from the figure, along with the increase of the concentration of rudiment Semen phaseoli radiati extract, hyaluronidase inhibitor also increases gradually, and the side shows that the rudiment Semen phaseoli radiati extract has the effect of Shu Min antiallergic.
3, the rudiment Semen phaseoli radiati extract is to propionibacterium acnes fungistatic effect figure
Adopt the Oxford agar diffusion method, the inhibition zone size of observing samples to propionibacterium acnes on flat board, thus reflection rudiment Semen phaseoli radiati extract is to the inhibition of acne pathogen.Mung Bean extract 10% concentration of three embodiment preparations is adopted in experiment, arrange three parallel, through bacteriostatic experiment, can find out, the Mung Bean extract has obvious bacteriostasis efficacy to propionibacterium acnes.Fig. 2 is that Mung Bean extract (10% concentration) (group 2) is to the fungistatic effect figure of propionibacterium acnes.
Clinical experiment of the present invention and result
Select volunteer's 60 examples, be responsive skin quality, allergies are arranged, and its face is all with acne symptom; The men and women does not limit, and tries out and clinical observation, is divided at random 2 groups, every group of 30 examples.Before observing, the clinical main manifestations of skin is that skin is responsive, and pruritus is uncomfortable, the long-term outbreak that has.
1, method: group 1: after clean of every day, on skin and easily send out the position to smear the emulsion of embodiment 4 preparations appropriate, each is once sooner or later.Group 2 (matched groups): after clean of every day, on skin and easily send out a position to smear commercially available certain brand Pimple removing scream appropriate, each is once sooner or later.Observed and statistical result in 3 days, 7 days, 1 month.
2, judgment criteria: (1) cures: clinical acne symptom disappears, reacts without skin sensitivity in one month again; (2) effective: clinical acne symptom alleviates, the skin sensitivity reactive moieties disappears or disappear, and reduces or reduces; (3) invalid: clinical acne symptom, skin sensitivity have no improvement.
3, result on trial: (1) group 1 instant effect, effective percentage can reach more than 93% in 3 days, 7 days, and certain cure rate, the cure rate of 1 month are arranged is more than 87%.Organize 2,3 days and 7 days effective percentage are that 47%, 1 month cure rate is 33% (2) all remarkable to the general responsive therapeutic effect of skin.(3) do not have an example reaction phenomenons such as irritated, that skin is uncomfortable to occur.
The inventor has also done the effect experiment of some other preparations, and as facial cream, emulsion and cosmetic water, final result shows that extract of the present invention has good Shu Min antiallergic effect, especially for the acne skin patient.
Claims (7)
1. the application of Mung Bean extract in the external use skin care of preparation moisturizing, Shu Min antiallergic, acne-removing, is characterized in that, described extract is obtained by following method extraction:
(1) get the rudiment Semen Phaseoli radiati Germinatus of two to four days, add deionized water according to the weight ratio of 1:2～1:4, carry out extract at room temperature with flash extracter, extraction time is 3～5 minutes, and speed is 4000～6000r/min;
(2) filter, centrifugal, get supernatant, add the 1,3 butylene glycol of 10wt%～20wt%, obtain the rudiment Semen phaseoli radiati extract after 80～90 ℃ of high temperature sterilizes.
2. application claimed in claim 1, is characterized in that, is filtered into 200～400 orders in described step (2) and filters.
3. application claimed in claim 1, is characterized in that, in described step (2), centrifugal rotational speed is 4000～5000rpm, and the centrifugal time is 10～20 minutes.
4. application claimed in claim 3, is characterized in that, described centrifugal rotational speed is 5000rpm, and the centrifugal time is 20 minutes.
5. application claimed in claim 1, is characterized in that, the described rudiment Semen Phaseoli radiati Germinatus of two to four days is prepared by following method:
(1) Semen phaseoli radiati is cleaned up, soak 6～10h;
(2) Semen phaseoli radiati after soaking was sprouted 2～4 days under 20 ℃～25 ℃, obtained described Semen Phaseoli radiati Germinatus.
6. application claimed in claim 5, is characterized in that, the Semen phaseoli radiati after soaking in described step (2) first drains away the water and is placed in and is lined with the cotton container of moistening dehydration, and covers with gauze, is placed in to cultivate under sprouting under 20 ℃～25 ℃ and sprouts; Keep Semen phaseoli radiati moistening between the budding period, every 24h cleans once with clear water, prevents that Semen Phaseoli radiati Germinatus is mouldy rotten; Germinating time is 2～4 days, after the end of germinateing, Semen Phaseoli radiati Germinatus is cleaned at least one times with clear water.
7. application claimed in claim 6, is characterized in that, keeping the moistening method of Semen phaseoli radiati between the budding period is every 2 hours trickles once.
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| CN102988242A (en) * | 2012-12-07 | 2013-03-27 | 上海相宜本草化妆品股份有限公司 | Method for preparing mung bean sprout extracting solution and application of mung bean sprout extracting solution |
| CN104095927A (en) * | 2013-04-08 | 2014-10-15 | 韦建学 | Eye refreshing agent used for eliminating and alleviating eye fatigue and malaise |
| CN103919231A (en) * | 2014-05-06 | 2014-07-16 | 恩施州益寿果业股份有限公司 | Preparation method of vitamin C-rich composite kiwi fruit powder |
| CN106726990B (en) * | 2016-12-29 | 2020-04-17 | 广州市中通生化制品有限公司 | Anti-allergy composition and preparation method and application thereof |
| CN115089529B (en) * | 2022-08-03 | 2023-07-25 | 肽源（广州）生物科技有限公司 | Mung bean hull fermentation product with acne removing effect, and preparation method and application thereof |
| CN115607489A (en) * | 2022-10-09 | 2023-01-17 | 皓雨(广州)化妆品制造有限公司 | Cosmetic raw material containing germination secretion and preparation method of secretion |
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