KR20190130340A - Cosmetic composition for allevation of acne with the extract of Lycopus lucidus or its fraction - Google Patents

Abstract

The present invention relates to a cosmetic composition for alleviating acne, comprising Lycopus lucidus extract or a fraction thereof. The present invention exerts an excellent effect on the prevention of acne.

Description

Cosmetic composition for improving acne comprising talan extract or a fraction thereof {Cosmetic composition for allevation of acne with the extract of Lycopus lucidus or its fraction}

The present invention is a lycra ( Lycopus lucidus ) relates to a cosmetic composition for improving acne comprising an extract or a fraction thereof.

The cause of acne has not yet been clearly identified, but hormonal action, excessive sebum secretion and bacterial infection is considered to be the cause. Acne is a complex inflammatory skin disease that occurs in the fur branch, hair follicles and surrounding tissues due to the above-mentioned causes.

Cotton cloth is a screw-like hardened sebum mass that appears most often on the face, forehead, and nose. Inside the cotton cloth, Propionibacterium is an anaerobic bacterium known as acne-causing bacteria. Acnes ) grow on the surface of the cotton wool , and the aerobic bacteria Staphylococcus epidermidis and Pityrosporum ovale grow on the surface of the cotton cloth, and various chemicals that produce them cause inflammation of hair follicles and surrounding skin. Will be.

Conventionally, oral or external preparations are used to treat the acne. Specifically, the focus is on exfoliation of pores using salicylic acid or retinoic acid derivatives of vitamin A derivatives and enhancement of bactericidal power against acne bacteria using antibiotics such as triclosan. However, salicylic acid is classified as a preservative in Korea and cannot contain more than 0.5% in the formulation, and the side effects of triclosan cause skin irritation when it comes in contact with the skin has been found concern about abuse of triclosan.

In recent years, as interest in skin care has increased, the occurrence of acne can lead to various mental diseases such as psychological pressure, loss of confidence, and depression. Therefore, it is urgent to prepare an effective method for treating acne with less side effects.

Republic of Korea Patent No. 10-1332215 (2013.11.18.), It describes a cosmetic composition for preventing acne and immune enhancement containing cedar and lotus leaf fermentation extract as an active ingredient. Korean Patent Registration No. 10-1827771 (2018.02.05.) Describes a cosmetic composition for preventing acne containing a mulberry extract.

The present invention is a lycra ( Lycopus Using lucidus ) extract or fractions thereof, it is intended to develop and provide a cosmetic composition for improving acne exhibiting the effect of improving or preventing acne.

The present invention is a lycra ( Lycopus lucidus ) provides a cosmetic composition for improving acne comprising an extract or a fraction thereof.

In the cosmetic composition for improving acne of the present invention, the fraction may be any one selected from hexane fraction, methylene chloride fraction, ethyl acetate fraction, n-butanol fraction.

In the cosmetic composition for improving acne of the present invention, the cosmetic composition is preferably Propionibacterium Acnes ( Propionibacterium Acnes ) is good to have an antimicrobial activity.

In the cosmetic composition for improving acne of the present invention, the cosmetic composition is preferably to suppress the production of nitric oxide (NO).

Thalan ( Lycopus) of the present invention Cosmetic composition containing lucidus ) extract or fractions thereof has an excellent effect on improving or preventing acne symptoms.

Figure 1 shows the results of human-derived cells (HDFn, HaCaT) toxicity of n-hexane, methylene chloride, ethyl acetate, n-butanol fraction, including the taxane extract.
Figure 2 is a result of evaluation of the inhibition of macrophage NO production of the lanthanum extract (A), n-hexane (B), methylene chloride (C), ethyl acetate (D), n- butanol (E) fraction thereof.

The present invention is a lycra ( Lycopus lucidus ) provides a cosmetic composition for improving acne comprising an extract or a fraction thereof.

Lycopus lucidus is a perennial herb belonging to the Lamiaceae family, also known as renovated or easily filamentous. Tacklan, which is mainly distributed in wet water, is widely distributed in Korea, China, Japan, and East Asia. The leaves and stems of talan are used as medicinal herbs, and the young shoots of early spring were also eaten. Taylan is a non-toxic plant that has a slightly warmer nature, and is sometimes tasted and sweetened and watered.

The extraction solvent of the taxane extract of the present invention may be any one or more selected from the group consisting of water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate and butyl acetate.

In the cosmetic composition for improving acne of the present invention, the fraction may be any one selected from hexane fraction, methylene chloride fraction, ethyl acetate fraction, n-butanol fraction. In addition, the fraction of the present invention may use a column purification method, the column may be, for example, an aromatic type synthetic adsorbent column.

In the cosmetic composition for improving acne of the present invention, the cosmetic composition is preferably Propionibacterium Acnes ( Propionibacterium It is good to have antimicrobial activity against Acnes ) and to suppress the production of nitric oxide (NO).

Propionibacterium of the present invention the taxlan extract and fractions thereof are acne-causing bacteria Acnes ) excellent antimicrobial activity, and confirmed that effectively inhibit the production of nitric oxide (NO) in macrophages, it was possible to develop a cosmetic composition that can prevent or improve acne symptoms.

On the other hand, in the cosmetic composition for improving acne of the present invention, the lycopene lucidus extract or a fraction thereof is preferably included in an amount of 0.10 to 80% by weight based on the total cosmetic composition. When the lycopus lucidus extract or fractions thereof are added in an amount of less than 0.10% by weight, there is a concern that the prevention or improvement effect of acne due to lycopus lucidus extract or fractions thereof contained in the cosmetic composition may not be sufficiently exhibited. In addition, when the composition of the Lycopus lucidus extract or fractions thereof is added in an amount of more than 80% by weight, even if the content of the Lycopus lucidus extract or fractions thereof increases in the cosmetic composition can be expected at a certain concentration or more The prevention or improvement effect of acne may not be enhanced any more, and because the content of fat in the formulation is not sufficient to maintain a stable formulation, a large amount of the formulation may collapse or the production cost may increase, resulting in a decrease in productivity. .

On the other hand, according to an embodiment of the present invention, the cosmetic composition of the present invention may be prepared in the formulation of oil-in-water emulsion, and may be prepared in any formulation commonly prepared in the art. For example, it can be formulated into solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing oils, powder foundations, emulsion foundations, wax foundations, packs, massage creams and sprays, etc. However, the present invention is not limited thereto, and more specifically, may be prepared in the form of a flexible lotion, a nourishing lotion, a nourishing cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, and a cleansing water.

Hereinafter, the content of the present invention will be described in more detail through the following examples and experimental examples. However, the scope of the present invention is not limited to the following Examples and Experimental Examples, but includes modifications of equivalent technical ideas.

< Example  One: Talc  Extracts and Its Fraction  Manufacture

500 g of dry talanchoe starch was ground to prepare a pulverized product. After 5000 g of 70% ethanol was added to the pulverized product, the extract was attached to a cooling capacitor and repeatedly extracted twice by heating at 80 ° C. for 3 hours. The extract was filtered with a 400 mesh filter cloth, and then again filtered with Whatman No. 2 filter paper.

Thereafter, the mixture was concentrated under reduced pressure at 55 ° C. in a distillation apparatus equipped with a cooling capacitor to obtain 78.4 g of a concentrate. Purified water was added to the obtained concentrate, followed by melting. Then, the solvent having different polarity in the fractional filter, n-hexane. The methylene chloride, ethyl acetate, n-butanol was repeated three times in the order of fractionation. The fractions were then concentrated under reduced pressure at 55 ° C. in a distillation apparatus equipped with a cooling capacitor, followed by n-hexane soluble fraction (7.45 g), methylene chloride soluble fraction (9.15 g), ethyl acetate soluble fraction (6.84 g), and n-. Butanol soluble fraction (16.35 g) was obtained respectively.

< Experimental Example  One: Talc  Extracts and Its Fraction  Cell Viability Assessment>

To determine the effect of taklan extract and its fraction on the cell viability, human dermal fibroblast neonatal (HDFn) and human keratinocyte (HaCaT) at 1 * 10 ⁴ cells / well After inoculation into 96-well plates, they were stabilized in a 37 ° C. incubator for 24 hours. Thereafter, each sample diluted by concentration was treated with cells, and then treated with MTT solution after 24 hours. After reacting for 2 hours, the sample was removed, and then dissolved in formazan (formazan) formed by adding 150 μl of DMSO to measure and analyze absorbance at 540 nm (FIG. 1). Figure 1 shows the results of human-derived cells (HDFn, HaCaT) toxicity of n-hexane, methylene chloride, ethyl acetate, n-butanol fraction, including the taxane extract.

As a result of the experiment, it was confirmed that talan extract and fractions thereof did not affect the viability of human normal fibroblasts and human keratinocytes at 100 ppm or less. From this, it was confirmed that the talan extract and fractions thereof of the present invention are harmless to the human body and have excellent safety.

< Experimental Example  2: Talc  Extracts and Its Fraction  Antimicrobial Activity Evaluation>

The strain used for antimicrobial activity was propionibacterium acnes cultured in plate medium, which was inoculated in a 10mL BHI liquid medium and incubated at 37 ° C for 24 hours. 0.2 μl of the activated test bacteria solution was added and then applied with a sterilized glass rod so as to spread evenly on the solid medium, and then measured by a disc plate method using agar plate disc. The antimicrobial activity evaluation results of the talan extract and its fractions are shown in Table 1 below. As a result of the experiment, it was confirmed that the talan extract and fractions thereof of the present invention had antibacterial activity in a concentration-dependent manner.

Clear zone (cm) sample Control Taylan Extract Hex fraction MC fraction EA fraction BuOH fraction process
density
(%) 1.0 0.00㎠ 0.00 ± 0.00 0.56 ± 0.18 0.32 ± 0.09 0.68 ± 0.09 0.00 ± 0.00 2.0 0.37 ± 0.15 0.84 ± 0.26 0.51 ± 0.18 0.93 ± 0.14 0.21 ± 0.08 4.0 0.98 ± 0.28 1.14 ± 0.35 0.78 ± 0.14 1.39 ± 0.07 0.56 ± 0.14 8.0 1.32 ± 0.13 1.28 ± 0.13 1.58 ± 0.27 1.72 ± 0.17 0.84 ± 0.13 Positive control 1.0% 1.84 ± 0.13

< Experimental Example  3: Talc  Extracts and Its Fraction  Inflammation Prevention Assessment>

The anti-inflammatory effect was evaluated by the effect of inhibiting the production of nitric oxide (NO). In a 48-well plate, RAW 264.7 cells, a mouse-derived macrophage line, were dispensed at 5 × 10 ⁴ cells / well, followed by incubation for 12 hours at 37 ° C. in a 5% CO ₂ incubator. After dilution, it was treated. Thereafter, lipopolysaccharide (lipopolysaccharide, LPS; Sigma-Aldrich), which was a positive control, was incubated at a concentration of 1 µg / ml for 24 hours, and then the culture supernatant was separated. 100 μl of the culture supernatant was treated with the same amount of Griess reagent and reacted in the dark for 10 minutes, and then the absorbance was measured at 517 nm using a micro-plate reader. The concentration of nitric oxide was calculated by comparison with a standard straight line obtained using sodium nitrite (NaNO ₂ ) (FIG. 2). Figure 2 is a result of evaluation of the inhibition of macrophage NO production of the lanthanum extract (A), n-hexane (B), methylene chloride (C), ethyl acetate (D), n- butanol (E) fraction thereof.

As a result, it was confirmed that the lanthanum extract and ethyl acetate fraction effectively inhibited the production of nitric oxide at 100 µg / ml and the other fractions at 30 µg / ml. From this, it was confirmed that the talan extract and fractions thereof of the present invention are excellent in preventing inflammation.

< Example  2 to 4, Comparative example  One: emulsion  Manufacturing of Base>

Emulsion base was prepared for the clinical trials on the acne prevention effect and skin stability of the cosmetics containing the talan extract and fractions thereof of the present invention was used in the following experiment. The composition of the emulsion base is shown in Table 2.

Ingredients (content: weight% ) Example  2 Example  3 Example  4 Comparative example  One Talan extract 10.0 - - - MC fraction - 10.0 - - EA fraction - - 10.0 - 1,3-butylene glycol 10.0 10.0 10.0 10.0 glycerin 5.1 5.1 5.1 5.1 Propylene glycol 4.2 4.2 4.2 4.2 Tocopheryl acetate 3.0 3.0 3.0 3.0 Liquid paraffin 4.6 4.6 4.6 4.6 Triethanolamine 1.0 1.0 1.0 1.0 Squalane 3.1 3.1 3.1 3.1 Macadamia Nut Oil 2.5 2.5 2.5 2.5 Polysorbate 60 1.6 1.6 1.6 1.6 Sorbitan sesquirrolate 1.6 1.6 1.6 1.6 Propylparaben 0.6 0.6 0.6 0.6 Carboxyl Vinyl Polymer 1.5 1.5 1.5 1.5 incense a very small amount a very small amount a very small amount a very small amount antiseptic a very small amount a very small amount a very small amount a very small amount Purified water Remaining amount Remaining amount Remaining amount Remaining amount system 100.0 100.0 100.0 100.0

< Experimental Example  4: Assessing Acne Prevention Levels>

In order to evaluate the degree of acne prevention, among the women aged 19 to 30, 20 acne was used for the emulsion base of Examples 2 to 4 and Comparative Example 1 in the same use method as the general lotion for 4 weeks. According to the opinion of the user, the degree of prevention was evaluated according to the following evaluation criteria. The degree of acne prevention is shown in Table 3 below.

sample 1 week 2 weeks 3 weeks 4 weeks Example  2 + + + ++ Example  3 + + ++ +++ Example  4 + ++ ++ +++ Comparative example  One ± ± ± ±

<Evaluation Criteria>

+++: Very good preventive effect.

++: Significantly preventive.

 +: Some prevention effect.

 ±: No preventive effect but no deterioration.

 -: No preventive effect, rather worse.

As a result of the experiment, it was confirmed that the talan extract (Example 2) and its fractions (Examples 3 and 4) have a very good acne prevention effect.

Claims (4)

Taeklan ( Lycopus lucidus ) Cosmetic composition for improving acne comprising an extract or a fraction thereof.
The method of claim 1,
The fraction is,
Acne improvement cosmetic composition, characterized in that any one selected from hexane fraction, methylene chloride fraction, ethyl acetate fraction, n-butanol fraction.
The method of claim 1,
The cosmetic composition,
Propionibacterium Acnes ) cosmetic composition for improving acne, characterized in that it has an antimicrobial activity.
The method of claim 3,
The cosmetic composition,
Acne improvement cosmetic composition, characterized in that to inhibit the production of nitric oxide (NO).

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