TWI535449B - Use of plectranthus amboinicus extract for preparing anti-acne composition - Google Patents

Description

Use of hand-flavored extract to prepare a composition for improving acne

The invention relates to a use of a hand-picked extract for preparing a composition for improving acne, in particular to a polyphenol-rich to the third extract of the scented scent, which has the functions of inhibiting bacteria, anti-oxidation and anti-inflammatory, so that it can be further Anti-acne cosmetic material composition or pharmaceutical composition for the purpose of improving or treating skin acne.

Acne, commonly known as acne, is a common skin problem that often causes problems in adolescents and adults, mainly due to hormone imbalance, bacterial infection, excessive sebum secretion, hair follicle sebaceous duct blockage and inflammatory response. Many factors are closely related; in addition, improper consumption or use of food or cosmetics may also cause acne. Obstructed hair follicles are prone to bacteria, such as Propionibacterium acnes and Staphylococcus aureus . The anaerobic acne bacteria that mainly parasitize on the skin can decompose sebum, causing pore keratinization, resulting in sebum accumulation. acne. Staphylococcus aureus infections often have pus formation, a purulent infection. When the body's defense ability is reduced, or when the stratum corneum is deficient in lipids, the equilibrium state changes, and the opportunistic microbial flora multiplies; the acne This disease can also be found in the lesion, which can cause folliculitis.

Current treatments for mild acne use water or a proper facial cleanser to remove excess oil and bacteria from the skin surface; treatments for more severe acne include Topical or oral antibiotics to kill bacteria and reduce inflammation, topical vitamin A acid (Retinoic acid) and its derivatives such as isotretinoin (Isotretinoin) to regulate the differentiation of epidermal keratinocytes to dissolve and excrete acne, topical rhododendron (Azelaic acid) to reduce the bacterial surface of the skin surface, hair follicles and sebaceous glands, topical fruit acid products to promote cell regeneration and accelerate the healing of acne scars, or external chemical synthesis agents to improve acne conditions, such as the Republic of China Patent Notice No. I329650 A remedy for the treatment of acne (acne acne) and the Republic of China Patent Publication No. 476648 discloses a "non-irritating composition for treating acne and other skin conditions", and the Republic of China Patent Publication No. 200519126 discloses a " Antibacterial agent against P. acnes, and Patent No. 200932205 of the Republic of China discloses a "external agent for skin for acne skin", etc.; however, there are still many defects in the above treatment methods, such as improper use of antibiotics, which may cause resistance, especially Antibiotic therapy has limited efficacy in adult acne; treatment with isotretinoin Although it can reduce the amount of sebum secretion, after stopping the drug, the amount of sebum secretion is restored, so that the number of bacteria is relatively increased; the use of rhododendron often causes local side erythema and tingling and other adverse side effects; although the effect of using fruit acid is significant, but also May cause over-stimulation and skin-sensitive side effects. Therefore, how to develop a better way to fight or treat acne has become the direction that inventors in the relevant field think.

Plectranthus amboinicus , also known as patchouli, stalk, musk, musk , etc., belongs to the perennial herb of the Lamiaceae family. It is mainly distributed in the tropical to subtropical zone of Asia. It has a special aroma and is extremely easy. Identification, stems and leaves are thick and crisp, and can be propagated by cutting. Because of the special aroma of hand scent, it is often used as a perfume ingredient, insect repellent or alternative medicines. In the past, it has been pointed out that essential oils derived from hand-flavored oil have the functions of regulating gastrointestinal, antibacterial, anti-inflammatory and analgesic effects, and carvacrol and thymol are strongly extracted from the leaves of hand-scented leaves. Anti-inflammatory and analgesic effects. The antibacterial effect of the hand-flavored fragrance, for example, in the Republic of China Patent Publication No. 201332583, discloses a method for producing an oral cleaning and care product containing a left-handed extract, and a composition thereof, which comprises the preparation of an oral care product containing a left-handed extract. The method utilizes steps of extraction, filtration, concentration, mixing, stirring, etc. to produce toothpaste or mites which have good inhibitory effects on Streptococcus mutans and Streptococcus sobrinus which cause tooth decay and plaque. Oral cleansing agents and health care products such as saliva; in addition, the anti-inflammatory effect of the hand-scented fragrance, for example, the Republic of China Patent Publication No. I320714 discloses a "plant extract for treating rheumatoid arthritis", which provides a left-handed fragrance ( Plectranthus Amboinicus Benth; PA) a pharmaceutical composition of crude extract or extract for the treatment of rheumatoid arthritis, and also for the preparation of a medicament for the treatment of rheumatoid arthritis using a crude extract or extract of the left-handed scent use.

In addition, the hand scent also has the effect of treating cancer or treating skin disorders. For example, the Republic of China Patent Publication No. I357334 and I399210 disclose "the scented leaf juice for treating cancer and/or tumor", which is mainly related to one kind of use. A composition for treating a tumor comprising an effective amount of leaf juice of Plectranthus amboinicus and providing a method of preparing the composition, and the composition for the manufacture of a medicament for treating hepatocellular carcinoma and/or melanoma And the method of preparing a plant extract for treating a skin condition and promoting wound healing, and mainly providing a method for preparing a Plectranthus amboinicus extract by a stirring separation method, The invention also relates to a pharmaceutical composition comprising a crude extract and/or extract of Pleurotus ostreatus which is useful for treating skin conditions, including promoting wound healing, particularly in diabetic patients.

However, at present, there are few researches on the treatment of acne, so the inventors used different extracts of hand-flavored extracts to compare their anti-decubitus ability, in order to develop better anti-acne related products.

Now, the inventor is considering the above-mentioned existing anti-acne method in actual implementation. There are still many deficiencies, so it is a tireless spirit, and it is improved by its rich professional knowledge and years of practical experience, and the invention is developed accordingly.

The main object of the present invention is to provide a use of a scented extract to improve the composition of acne, which means that the third extract rich in polyphenols has the ability to inhibit bacteria, anti-oxidation and anti-inflammatory, so it can be further As a cosmetic material composition or pharmaceutical composition for improving or treating acne.

In order to achieve the above-mentioned object, the present invention provides a use of a scented extract to prepare a composition for improving acne, which is administered at an effective dose (preferably 0.3 to 10 mg/mL, more preferably 0.3 to 5 mg/mL). The extract of the hand scent is applied to the skin to scavenge free radicals, inhibit nitric oxide production, and fight against acne bacteria and Staphylococcus aureus.

The invention also provides a preparation method of the scented extract of scented scented acne, comprising the following steps: Step 1: adding the first organic solvent to the scented material to extract at room temperature to obtain a first extract. Step 2: Performing a partitioning extraction, dispersing the components by using an immiscible solvent, and dividing the first extract with a second organic solvent to obtain a first organic phase and a first aqueous phase. Step 3: The first organic phase is separated by a vacuum, leaving only the component mixture to be concentrated, and then a third organic solvent is added for partitioning to divide a second organic phase and a third organic phase, wherein the second organic phase has a second extract having a low polarity, the third organic phase having a third extract rich in polyphenols; and step 4: the first aqueous phase is further distributed with the fourth organic solvent to define a fourth organic phase And a second aqueous phase, wherein the fourth organic phase has a fourth extract having a high polarity, and the second aqueous phase contains a fifth extract of extremely high polarity; wherein the third extract has the best anti-inflammatory and acne-promoting utility.

In one embodiment of the present invention, the first organic solvent is a 95% by volume ethanol solution, the second organic solvent is a water/ethyl acetate solution having a volume ratio of 1:2, and the third organic solvent is a volume ratio of 1: A solution of 1 in n-hexane/95% methanol; the first organic phase is the ethyl acetate phase, the second organic phase is the n-hexane phase, and the third organic phase is the methanol phase.

Accordingly, the extract of the hand-scented extract can be further used as a cosmetic material composition or a pharmaceutical composition for anti-inflammatory and acne-promoting.

(S1)‧‧‧Step one

(S2)‧‧‧Step 2

(S3) ‧ ‧ Step 3

First Figure: Flowchart of the step of extracting the hand-paste of the preferred embodiment of the present invention

Second Figure: Flowchart of the step of extracting the hand fragrance according to a specific embodiment of the present invention

Figure 3: Test chart of the antibacterial ability of the extract to the acne

Figure 4: Test chart for the antibacterial ability of the extract of the hand-scented extract against Staphylococcus aureus

Figure 5: Effect of the first extract of Zhixiang on the production of nitric oxide

Figure 6: Effect of the third extract of Zhixiang on the production of nitric oxide

Figure 7: Effect of the first extract of Zhixiang on cell viability

Figure 8: Effect of the third extract of Zhixiang on cell viability

The purpose of the present invention and its structural and functional advantages will be as shown in the following figures. The structure is described in conjunction with the specific embodiments, so that the reviewing committee can have a deeper and more specific understanding of the present invention.

The invention relates to the use of the extract of the hand-scented extract to prepare a composition for improving acne, which is applied to the skin by an effective dose of 0.3~10 mg/mL (more preferably 0.3-5 mg/mL) to the skin extract. scavenging free radicals, inhibition of NO production, and anti acnes (P. acnes) and Staphylococcus aureus (S.aureus).

The invention also discloses a preparation method of the extract of the hand-paste extract for improving the use of acne. Please refer to the first figure, which is a flow chart of the step of extracting the hand-paste of the preferred embodiment of the present invention, comprising: step one (S1): utilizing the first An organic solvent (for example, 95% by volume ethanol solution) is extracted to the hand-paste material to obtain a first extract; and step 2 (S2): a second organic solvent is used (for example, water at a volume ratio of 1:2/ Extracting the first extract with an ethyl acetate solution to divide a first aqueous phase and a first a machine phase (for example, an ethyl acetate phase); and a third step (S3): removing the first organic phase solvent by a vacuum concentrator, and then adding a third organic solvent (for example, hexane/95 in a volume ratio of 1:1) The % methanol solution is partitioned to extract a second organic phase (for example, a n-hexane phase) and a third organic phase (for example, a methanol phase), wherein the third organic phase has a third extract, and the third extraction It has anti-inflammatory and acne-promoting effects.

In addition, the scope of the invention may be further exemplified by the following specific examples, which are not intended to limit the scope of the invention.

Example 1: Preparation of aroma extract

To the hand- picked material Plectranthus amboinicus (Lour.) Spreng was provided by Dr. Qiu Shuyuan from Hualien Agricultural Improvement Field and Cai Yuexia. The flow chart of the step of extracting the hand scent according to a specific embodiment of the present invention is as shown in the second figure. First, the leaves of the hand scent are washed, dried, and weighed, and the whole leaves are screened and dried at 35-40 ° C; The dried leaves of the hand scent were ground to a powder to obtain a total weight of 1.58 kg, and the mixture was continuously extracted 5 times with ethanol (first organic solvent) at room temperature, and the supernatant was collected and filtered, and concentrated to obtain an extract. The first extract was approximately 130.0 g. Then, with water (H ₂ O) and ethyl acetate (Ethyl acetate, EA) in a ratio of 1:2 (second organic solvent) as a partition extraction, respectively, an EA layer (first organic phase) and a H ₂ O layer were obtained. An aqueous phase) in which the EA layer (first organic phase) was subjected to removal of ethyl acetate using a reduced pressure concentrator, and the H ₂ O layer (first aqueous phase) had an H ₂ O layer extract (first residue). Further, the ethyl acetate layer from which the solvent was removed was subjected to partition extraction by using n-Hexane and 95% methanol (MeOH) in a ratio of 1:1 (third organic solvent) to obtain an n-Hexane layer (the first). The two organic phase) and the MeOH layer (third organic phase), wherein the MeOH layer had a second extract of 22.3 g and the n-Hexane layer had a second extract of 27.4 g. In addition, the first residue of the H ₂ O layer (first aqueous phase) is further subjected to partition extraction using n-butanol (n-BuOH) and water (H ₂ O) in a ratio of 1:1 (fourth organic solvent). Obtaining an n-BuOH layer (fourth organic phase) and a H ₂ O layer (second aqueous phase), wherein the n-BuOH layer (fourth organic phase) has a fourth extract of 31.5 g and a H ₂ O layer ( The second aqueous phase had a fifth extract of 46.7 g.

Example 2: Analysis of antioxidant components and antioxidant capacity of extracts from hand-flavored

(1) Determination of total polyphenol content

The analysis of antioxidant components uses Folin-ciocalteu reagent to test the phenolic substances in the sample, which can be reduced to a blue complex by itself, and has the largest absorption peak at OD620. The higher the absorbance value, the total polyphenol content in the sample. The higher. Pre-formulated 2% sodium carbonate (Na ₂ CO ₃ ) and 50% Folin-ciocalteau reagents are each dissolved in ddH ₂ O for later use, using gallic acid as standard, concentration 0, 20 40, 60, 80, 100 μg/ml. After adding ddH ₂ O, gallic acid, or adding different concentrations to the hand-flavored extract, add 10 μL of Folin-ciocalteau reagent for 30 minutes, add 200 μL of sodium carbonate and mix and react at room temperature. For ~5 minutes, the OD620 can be measured as a standard curve. The results show how many micrograms of gallic acid/mg of P.cablin dw are contained per milligram of the hand fragrance.

(2) Determination of DPPH free radical scavenging ability

DPPH (1,1-diphenyl-2-picrylhydrazy) is a simple anti-free radical method. The experiment utilizes the ability of antioxidants to provide hydrogen ions and the ability to scavenge free radicals to carry out the antioxidant capacity of the hand. Dilute to different concentrations of the extract to the scented extract by DPPH, add 100μM DPPH, mix and put in a 37 °C water bath to avoid the light reaction for 30 minutes, and finally use the spectrophotometer to measure OD517, and make a calibration curve to calculate the free radicals. The inhibition rate was 50% (IC ₅₀ ), and the inhibition rate was calculated as follows: DPPH scavenging (%) = [1 - (experimental absorbance / control absorbance) x 100%].

(3) Total antioxidant capacity

The total antioxidant capacity analysis experiment was carried out by using 2,2'-Azino-bis (3-Ethylbenzthiazoline-6-Sulfonic Acid), abbreviated as ABTS, by hydrogen peroxide (H ₂ O ₂ ) and peroxidase. ABTS ⁺ cationic free radicals, to be stable blue-green, added to the sample, if there is the ability to remove ABTS ⁺ cationic free radicals, the color will become shallower and the absorbance will decrease. When calculating the total antioxidant capacity, the water-soluble vitamin E (Trolox) was used as a control standard to prepare a standard curve and evaluate the total antioxidant capacity of each extract, which is called water-soluble vitamin E equivalent antioxidant capacity (Trolox Equivalent Antioxidant Capacity). ;TEAC). A mixed solution of 7 mM ABTS and 2.45 mM potassium persulfate (K ₂ O ₈ S ₂ ) in ABTS ^{+ was} pre-formed and protected from light for 18-24 hours until the reaction was completely blue-green and had a maximum absorption peak at 743 nm. 90 μL of ABTS ⁺ and 10 μL of the mixture were added to the hand-flavored extract or trolox, and reacted at room temperature for 45 seconds, and the OD734 absorbance was measured.

The results of total polyphenol content determination and antioxidant capacity analysis are shown in Table 1. The total polyphenol content in the third extract of the hand-flavored extract is the highest, about 6~12 times that of other extracts; the total amount of the fifth extract is more The phenol content is the lowest. The DPPH free radical semi-inhibitory concentration test showed that the semi-inhibitory concentration of DPPH free radical of the first extract was 176±20 μg/ml, the third extract was 27±0.00 μg/ml, and the fourth extract was 272±50 μg/ml. The second extract and the fifth extract were both greater than 500 μg/mL, and it was found that the third extract having the highest total polyphenol content had the most obvious effect of scavenging DPPH radicals, followed by the first extract. The semi-inhibitory concentration test of ABTS free radicals showed that the semi-inhibitory concentration of the first extract ABTS radical was 129±10 μg/ml, the third extract was 21±0.00 μg/ml, and the fourth extract was 325±20 μg/ml. The second extract and the fifth extract were both greater than 500 μg/mL, indicating that the third extract had the best effect of clearing the ABTS radical, while the second extract and the fifth extract had the weakest scavenging effect.

Example 3: Antibacterial activity test of the extract to the hand

The strains Staphylococcus aureus BCRC 12657 and Propionibacterium acnes BCRC10723 used in the experiments were purchased from the Bioresource Collection and Research Center (BCRC). Tube activation was performed using the method recommended by the BCRC to verify the purity and activation of the species. The activated cells are all transferred to the designated liquid medium and cultured at the specified temperature.

(1) The bacteriostasis activity test was carried out by a disk-diffusion method, and different concentrations of the extract of the scented scented scented extract were placed on a filter paper in an appropriate solvent for use to prepare a Mueller Hinton Agar (MHA) plate, and 0.2 mL of the bacterium was prepared. The solution is evenly applied to the surface of the medium, and after standing for 3 to 5 minutes, a paper ingot (8 mm) containing the concentration of 5 mg or 10 mg to the hand-flavored extract (the first extract or the third extract) is placed, and the medium is placed. On the surface, cultured at 37 ° C for 24 hours, observe and record the Zone of inhibition formed around the filter paper; if there is a significant inhibitory effect, a suppression loop will be produced, and the size of the inhibition loop measured by the antibiotic activity is Calculated in terms of the distance between its diameter and the outer ring of the zone of inhibition, expressed in mm (mm).

For the results, please refer to the third figure, the test chart of the antibacterial ability of the hand-flavored extract to the acne bacillus. The above picture shows the antibacterial ability of the first extract of the scented scented acne to the acne bacillus. Add 5 mg of the first extract to suppress the diameter of the ring by 10 mm. And add 10mg of the first extract The diameter of the object is 13mm; the figure below shows the antibacterial ability of the third extract of the scented scented bacillus against the acne bacillus, adding 5mg of the third extract, which has the effect of inhibiting the acne bacillus, the inhibition circle is 16mm, and the third extract of 10mg is added. The suppression ring is significantly increased to 26mm. The results show that when the third extract is used, the diameter of the inhibition ring increases as the concentration increases, so the third extract does have an excellent effect of inhibiting the acne bacillus.

Please refer to the fourth figure, the test chart of the antibacterial ability of the extract of the hand-flavored extract against Staphylococcus aureus. The above figure shows the antibacterial ability of the first extract of the hand-flavored extract against Staphylococcus aureus, and the first extract of 5 mg is added. Staphylococcus aureus has no obvious inhibitory effect; the following picture shows the antibacterial ability of the third extract of the hand-flavored grape against Staphylococcus aureus, adding 5mg of the third extract, which has the effect of inhibiting the acne bacillus, the inhibition circle is 28mm . The results show that the third extract does have an excellent inhibitory effect on S. aureus.

(2) Testing the minimum inhibitory concentration of the first extract and the third extract against Acne and Staphylococcus aureus

The experimental strain was activated and cultured for 18 to 24 hours, and the activated liquid was used to measure the absorbance of OD595. The diluted solution was used to dilute the bacterial solution to 10 ⁵ to 10 ⁶ CFUs/mL, and the total number of bacteria was counted on the plate. Then, take 180 μl of the diluted bacterial solution into a 96-well culture dish, place 20 μl of the control group and the test sample (the first extract and the third extract), measure the OD595 and record it, and then place the fungus tray at 37 ° C. In the incubator culture, Staphylococcus aureus is cultured for 18 to 24 hours, and acne bacteria are cultured for 72 hours.

The results are shown in Table 2. The minimum inhibitory concentration of the third extract is lower than that of the first extract. The minimum inhibitory concentration of the third extract for Staphylococcus aureus is 0.3 mg/mL, and the minimum for the acne bacillus. The inhibitory concentration is 0.5 mg/mL, so the third extract has a better bacteriostatic effect.

Example 4: Anti-inflammatory ability test of the hand extract

The mouse macrophage RAW264.7 (BCRC60001) used was purchased from the BCRC Bioresource Conservation and Research Center, which is a mouse macrophage elicited by BALB/c mice with Abelson murine leukemia virus. The cell growth condition DMEM contains 10% fetal bovine serum (FBS), and is placed in a constant temperature cell culture incubator at 37 ° C, 5% CO ₂ , and the cells are grown for about 2 to 3 days to 8 to 9 minutes. Subculture is then carried out.

(1) Determination of nitric oxide produced by cells

The nitric oxide (NO) produced in the cell is oxidized to nitrite (NO ^2- ) and nitric acid (nitate, NO ^3- ), so the method of measuring nitrite can be used. Indirect determination of the amount of nitric oxide produced. The Griess reagent used in this experiment reacts with nitrite to form a red solution with a maximum absorption peak at OD550. The concentration of nitrite in the cell culture medium to be tested will be different, and the absorbance will be different. . Firstly, 100 μL/well of macrophages (5× ^{10 4} cell/well) were placed in a 96-well culture dish and cultured at 37 ° C for 24 hours. After the cells were attached, the new culture medium was replaced and designed according to the experiment. Add 1 μL per well to different concentrations (25 or 50 μg/mL) of the palmetto extract (first extract or third extract), react for 30 minutes, then add LPS (10 ng/ml) for 20 hours, add, etc. The amount of Griess reagent (sigma) was 100 μL/well, shaking for 5 minutes. The absorbance was detected by ELISA reader at a wavelength of 550 nm, and sodium nitrite (NaNO ₂ ) was used as a standard calibration curve to determine the release amount of nitrite. .

The results are shown in the fifth figure. The cells are not induced by LPS. 1.643±0.604μM, and cells added LPS (10ng/mL) alone for 24 hours will induce a large amount of nitric oxide production, the value is 19.615±0.702μM; cells added LPS (10ng/mL) and different concentrations to the first extract of the hand-flavor It does not significantly inhibit nitric oxide as the dose increases. Please refer to the sixth figure. When adding the third extract of 25μg/mL, the amount of nitric oxide is reduced to 11.092±0.721μM. When adding the third extract of 50μg/mL, the amount of nitric oxide is reduced to 3.017±. 1.313 μM. From this, it can be seen that the amount of nitric oxide decreases as the dose of the added third extract increases, so the third extract has anti-inflammatory effects, while the first extract has no significant effect.

(2) Cell viability analysis

The mouse macrophage RAW264.7 (5x10 ⁴ cells/well), which has been quantified to 100 μL/well, was placed in a 96-well culture plate and cultured at 37 ° C for 24 hours. After the cells were attached, a new culture solution was replaced. Experimental design was carried out by adding different concentrations (25 or 50 μg/mL) to the extract of the hand extract (the first extract or the third extract) for 30 minutes, and then adding LPS (10 ng/mL) for 24 hours, removing the medium and adding the MTT solution. (0.5 mg/mL in PBS solution), and reacted at 37 ° C for 3 hours. After centrifugation for 5 minutes and removal of the supernatant, the crystals were dissolved by adding 100 μL/well of DMSO, shaken for 5 minutes, and finally OD550 was detected using an ELISA reader. The interpretation formula is as follows: cell viability (%) = (experiment group absorbance value / control absorbance value x 100%).

The results are shown in the seventh figure. Cells alone after adding LPS (10 ng/mL) for 24 hours will cause cell viability to decrease. The cell survival rate of cells added with LPS (10 ng/mL) and different concentrations to the first extract of the scent of the scent is 80. Above %, there is no significant difference. Please refer to the eighth figure. Cells added to LPS (10 ng/mL) alone will cause cell viability after 24 hours; cells added LPS (10 ng/mL) and added 25 μg/mL of the third extract with a cell viability of 72.27. ±4.811%; the addition of 50μg/mL of the third extract had a cell viability of 94.02%±0.291, indicating that the third extract had no toxic reaction to the cells, and the third extract at 50 μg/mL inhibited LPS-induced cell viability. Decline, increase fine Cell viability.

It has been confirmed by the above experiments that the extract of the hand-scented extract (especially a third extract rich in polyphenols) not only has the ability to inhibit bacteria, but also has the ability of anti-oxidation and anti-inflammatory; thereby, the extract to the hand-flavor can be Further, it is used as a cosmetic material composition, a skin care product or a pharmaceutical composition for anti-acne, and the above composition can be used in combination with a skin external preparation. The above-mentioned "skin external preparation" means a topical ingredient which is usually used in cosmetics or pharmaceuticals, including, but not limited to, other whitening agents, moisturizers, antioxidants, ultraviolet absorbers, surfactants, thickeners. The coloring material, the skin nutrient, and the like can be, for example, a component of the concentrated essence or added to the mask, and the user can be applied to the skin in an appropriate amount to prevent the occurrence of acne or slow down the development of acne.

It is worth noting that the "to the hand extract" (specifically "the third extract") of the present invention uses 0.3 to 10 mg/mL because of the minimum inhibitory concentration of the third extract against Staphylococcus aureus and acne bacteria. 0.3mg/mL and 0.5mg/mL respectively, so it is difficult to achieve obvious antibacterial effect when the dose is less than 0.3mg/mL, while the third extract with dose greater than 10mg/mL has excellent antibacterial effect, but it is used. The above may cause skin irritation, redness, allergies and the like of the user, or increase the cost; therefore, the preferred dosage of the extract to the hand-scented extract in the present invention is 0.3-10 mg/mL.

It can be seen from the above description that the present invention has the following advantages compared with the prior art:

1. The present invention proves by scientific research that the extract of the hand-flavored extract not only has the ability to inhibit Staphylococcus aureus and Acne bacillus, but also has anti-oxidation and anti-inflammatory ability, so the use of the hand-flavored extract as a cosmetic material composition for improving acne, The best results can be achieved with skin care products or pharmaceutical ingredients.

2. The hand-picked extract of the present invention is extracted from a natural plant and is not toxic to cells, and can avoid the occurrence of drug resistance or irritation to the skin compared to the conventional acne treatment method.

In summary, the use of the scented extract of the present invention in the preparation of a composition for improving acne can indeed achieve the intended efficacy by the above-disclosed examples, and the present invention has not been disclosed before the application. Cheng has fully complied with the requirements and requirements of the Patent Law.爰Issuing an application for a patent for invention in accordance with the law, and asking for a review, and granting a patent, is truly sensible.

The illustrations and descriptions of the present invention are merely preferred embodiments of the present invention, and are not intended to limit the scope of the present invention; those skilled in the art, which are characterized by the scope of the present invention, Equivalent variations or modifications are considered to be within the scope of the design of the invention.

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Claims (8)

A use of a hand-scented extract for the preparation of a composition for improving acne, which is applied to an effective dose of a hand-paste extract to the skin to scavenge free radicals, inhibit nitric oxide production, and to fight against Propionibacterium acnes and Staphylococcus aureus ; wherein the extract to the hand- paste is obtained by the following steps: Step 1: extracting a material of Plectranthus amboinicus with a first organic solvent to obtain a first extract, Wherein the first organic solvent is a 95% by volume ethanol solution; Step 2: extracting the first extract with a second organic solvent to define a first aqueous phase and a first organic phase, wherein the second organic The solvent is a water/ethyl acetate solution having a volume ratio of 1:2; and the third step: removing the solvent of the first organic phase, and extracting with a third organic solvent to divide a second organic phase and a third organic phase. Wherein the third organic solvent is a 1:1 ratio of n-hexane/95% methanol solution, and the third organic phase has a third extract, and the third extract Take anti-inflammatory and improve acne effect. The use according to claim 1, wherein the effective dose is 0.3 to 10 mg/mL. The use according to claim 2, wherein the effective dose is 0.3 to 5 mg/mL. The use of claim 1, wherein the first organic phase is an ethyl acetate phase, the second organic phase is a n-hexane phase, and the third organic phase is a methanol phase. The use according to the first aspect of the invention, wherein the hand extract is a cosmetic material composition or a pharmaceutical composition having anti-inflammatory and acne-promoting properties. A method for preparing a scented extract of acne for improving the use of acne comprises: step 1: extracting a scented material with a first organic solvent to obtain a first extract, wherein the first organic solvent is 95% by volume Ethanol solution; Step 2: extracting the first extract with a second organic solvent to divide a first aqueous phase and a first organic phase, wherein the second organic solvent is a water/acetic acid ratio of 1:2 by volume And a third organic solvent and a third organic phase, wherein the third organic solvent is a volume ratio of 1 : 1 n-hexane / 95% methanol solution, and the third organic phase has a third extract, and the third extract has anti-inflammatory and acne-promoting effects. The method of claim 6, wherein the first organic phase is an ethyl acetate phase, the second organic phase is a n-hexane phase, and the third organic phase is a methanol phase. The method of claim 6, wherein the third extract is a cosmetic material composition or a pharmaceutical composition having anti-inflammatory and acne-promoting properties.